HO065824

EST Overview


Name	HO065824
Unique Name	HO065824
Type	EST
Organism	Cicer arietinum (chickpea)
Sequence length	270

Libraries


Library Name	Type
LIBEST_026412 Chickpea drought stressed cDNA SSH library AS1	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Cicer arietinum unigene v1	Cicer arietinum unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Cicer arietinum unigene v1	2010-12-27
Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide	2011-02-03
Homology Analysis: Cicer arietinum unigene v1 vs Swissprot	2010-12-28
Homology Analysis: Cicer arietinum unigene v1 vs Trembl	2010-12-28
InterProScan analysis for Cicer arietinum unigene v1	2010-12-29

Homology

[Close]

BLAST of HO065824 vs. SwissProt
Match: RL322_ARATH (60S ribosomal protein L32-2 OS=Arabidopsis thaliana GN=RPL32B PE=2 SV=1)

HSP 1 Score: 65.4698 bits (158), Expect = 9.924e-11
Identity = 33/51 (64.71%), Postives = 36/51 (70.59%), Query Frame = -1

Query:   19 MAVPLLTXXXXXXXXXXXXRPQSDRKVSVKPSWRRPKGIDPRVRRKFKGCT 171
            MAVPLLT            RPQSDR+++VK SWRRPKGID RVRRKFKG T
Sbjct:    1 MAVPLLTKKVVKKRSAKFIRPQSDRRITVKESWRRPKGIDSRVRRKFKGVT 51

[Close]

BLAST of HO065824 vs. SwissProt
Match: RL321_ARATH (60S ribosomal protein L32-1 OS=Arabidopsis thaliana GN=RPL32A PE=2 SV=2)

HSP 1 Score: 65.4698 bits (158), Expect = 9.924e-11
Identity = 33/51 (64.71%), Postives = 36/51 (70.59%), Query Frame = -1

Query:   19 MAVPLLTXXXXXXXXXXXXRPQSDRKVSVKPSWRRPKGIDPRVRRKFKGCT 171
            MAVPLLT            RPQSDR+++VK SWRRPKGID RVRRKFKG T
Sbjct:    1 MAVPLLTKKVVKKRSAKFIRPQSDRRITVKESWRRPKGIDSRVRRKFKGVT 51

[Close]

BLAST of HO065824 vs. TrEMBL
Match: Q0J4B8_ORYSJ (Os08g0524600 protein (Fragment) OS=Oryza sativa subsp. japonica GN=Os08g0524600 PE=4 SV=1)

HSP 1 Score: 73.1738 bits (178), Expect = 9.539e-12
Identity = 39/69 (56.52%), Postives = 43/69 (62.32%), Query Frame = -1

Query:   19 PRCLFTSHEHRFNSSREKMAVPLLTXXXXXXXXXXXXRPQSDRKVSVKPSWRRPKGIDPRVRRKFKGCT 225
            PR L  +   RF    E+MAVPLLT            RP SDR + +KPSWRRPKGID RVRRKFKGCT
Sbjct:    1 PRLLPPNSSLRFPLRSEEMAVPLLTKKIVKKRVKQFKRPHSDRYLCLKPSWRRPKGIDSRVRRKFKGCT 69

[Close]

BLAST of HO065824 vs. TrEMBL
Match: C6SZ81_SOYBN (Putative uncharacterized protein OS=Glycine max PE=2 SV=1)

HSP 1 Score: 73.1738 bits (178), Expect = 9.539e-12
Identity = 36/51 (70.59%), Postives = 38/51 (74.51%), Query Frame = -1

Query:   19 MAVPLLTXXXXXXXXXXXXRPQSDRKVSVKPSWRRPKGIDPRVRRKFKGCT 171
            MAVPLL+            RPQSDRK+SVKPSWRRPKGID RVRRKFKGCT
Sbjct:    1 MAVPLLSKKIVKKRVKKFIRPQSDRKISVKPSWRRPKGIDSRVRRKFKGCT 51

[Close]

BLAST of HO065824 vs. TrEMBL
Match: C6SXB4_SOYBN (Putative uncharacterized protein OS=Glycine max PE=2 SV=1)

HSP 1 Score: 73.1738 bits (178), Expect = 9.539e-12
Identity = 36/51 (70.59%), Postives = 38/51 (74.51%), Query Frame = -1

Query:   19 MAVPLLTXXXXXXXXXXXXRPQSDRKVSVKPSWRRPKGIDPRVRRKFKGCT 171
            MAVPLL+            RPQSDRK+SVKPSWRRPKGID RVRRKFKGCT
Sbjct:    1 MAVPLLSKRIVKKRVKKFIRPQSDRKISVKPSWRRPKGIDSRVRRKFKGCT 51

[Close]

BLAST of HO065824 vs. TrEMBL
Match: B9I117_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_660564 PE=4 SV=1)

HSP 1 Score: 71.633 bits (174), Expect = 2.775e-11
Identity = 36/51 (70.59%), Postives = 37/51 (72.55%), Query Frame = -1

Query:   19 MAVPLLTXXXXXXXXXXXXRPQSDRKVSVKPSWRRPKGIDPRVRRKFKGCT 171
            MAVPLLT            RPQSDRK+SVK SWRRPKGID RVRRKFKGCT
Sbjct:    1 MAVPLLTKKIVKKRVKKFKRPQSDRKISVKTSWRRPKGIDSRVRRKFKGCT 51

[Close]

BLAST of HO065824 vs. TrEMBL
Match: A9PBE9_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_665064 PE=2 SV=1)

HSP 1 Score: 70.4774 bits (171), Expect = 6.183e-11
Identity = 35/51 (68.63%), Postives = 37/51 (72.55%), Query Frame = -1

Query:   19 MAVPLLTXXXXXXXXXXXXRPQSDRKVSVKPSWRRPKGIDPRVRRKFKGCT 171
            MAVPLLT            RPQSDRK+SVK +WRRPKGID RVRRKFKGCT
Sbjct:    1 MAVPLLTKKIVKKRVKKFKRPQSDRKISVKTNWRRPKGIDSRVRRKFKGCT 51

[Close]

BLAST of HO065824 vs. TrEMBL
Match: B9GQP4_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_711534 PE=4 SV=1)

HSP 1 Score: 70.0922 bits (170), Expect = 8.075e-11
Identity = 34/51 (66.67%), Postives = 37/51 (72.55%), Query Frame = -1

Query:   19 MAVPLLTXXXXXXXXXXXXRPQSDRKVSVKPSWRRPKGIDPRVRRKFKGCT 171
            MA+PLLT            RPQSDRK+SVK +WRRPKGID RVRRKFKGCT
Sbjct:    1 MAIPLLTKKIVKKRVKKFKRPQSDRKISVKTNWRRPKGIDSRVRRKFKGCT 51

[Close]

BLAST of HO065824 vs. TrEMBL
Match: A9PDT0_POPTR (Putative uncharacterized protein OS=Populus trichocarpa PE=2 SV=1)

HSP 1 Score: 70.0922 bits (170), Expect = 8.075e-11
Identity = 34/51 (66.67%), Postives = 37/51 (72.55%), Query Frame = -1

Query:   19 MAVPLLTXXXXXXXXXXXXRPQSDRKVSVKPSWRRPKGIDPRVRRKFKGCT 171
            MA+PLLT            RPQSDRK+SVK +WRRPKGID RVRRKFKGCT
Sbjct:    1 MAIPLLTKKIVKKRVKKFKRPQSDRKISVKTNWRRPKGIDSRVRRKFKGCT 51

[Close]

BLAST of HO065824 vs. TrEMBL
Match: Q9XFE3_ORYSA (60S ribosomal protein (Fragment) OS=Oryza sativa PE=2 SV=1)

HSP 1 Score: 69.707 bits (169), Expect = 1.055e-10
Identity = 35/56 (62.50%), Postives = 37/56 (66.07%), Query Frame = -1

Query:   19 SSREKMAVPLLTXXXXXXXXXXXXRPQSDRKVSVKPSWRRPKGIDPRVRRKFKGCT 186
            S R KMAVPLLT            RP SDR + +K SWRRPKGID RVRRKFKGCT
Sbjct:    6 SDRSKMAVPLLTKKIVKKRVKQFKRPHSDRYIGLKTSWRRPKGIDSRVRRKFKGCT 61

[Close]

BLAST of HO065824 vs. TrEMBL
Match: Q0J0R0_ORYSJ (Os09g0501700 protein (Fragment) OS=Oryza sativa subsp. japonica GN=Os09g0501700 PE=4 SV=2)

HSP 1 Score: 69.707 bits (169), Expect = 1.055e-10
Identity = 35/56 (62.50%), Postives = 37/56 (66.07%), Query Frame = -1

Query:   19 SSREKMAVPLLTXXXXXXXXXXXXRPQSDRKVSVKPSWRRPKGIDPRVRRKFKGCT 186
            S R KMAVPLLT            RP SDR + +K SWRRPKGID RVRRKFKGCT
Sbjct:   22 SDRSKMAVPLLTKKIVKKRVKQFKRPHSDRYIGLKTSWRRPKGIDSRVRRKFKGCT 77

[Close]

BLAST of HO065824 vs. TrEMBL
Match: B9G4E8_ORYSJ (Putative uncharacterized protein OS=Oryza sativa subsp. japonica GN=OsJ_29906 PE=4 SV=1)

HSP 1 Score: 69.707 bits (169), Expect = 1.055e-10
Identity = 35/56 (62.50%), Postives = 37/56 (66.07%), Query Frame = -1

Query:   19 SSREKMAVPLLTXXXXXXXXXXXXRPQSDRKVSVKPSWRRPKGIDPRVRRKFKGCT 186
            S R KMAVPLLT            RP SDR + +K SWRRPKGID RVRRKFKGCT
Sbjct:   33 SDRSKMAVPLLTKKIVKKRVKQFKRPHSDRYIGLKTSWRRPKGIDSRVRRKFKGCT 88

[Close]

BLAST of HO065824 vs. TAIR peptide
Match: AT5G46430.2 (| Symbols: | Ribosomal protein L32e | chr5:18833429-18834409 FORWARD LENGTH=133)

HSP 1 Score: 65.4698 bits (158), Expect = 7.772e-12
Identity = 33/51 (64.71%), Postives = 36/51 (70.59%), Query Frame = -1

Query:   19 MAVPLLTXXXXXXXXXXXXRPQSDRKVSVKPSWRRPKGIDPRVRRKFKGCT 171
            MAVPLLT            RPQSDR+++VK SWRRPKGID RVRRKFKG T
Sbjct:    1 MAVPLLTKKVVKKRSAKFIRPQSDRRITVKESWRRPKGIDSRVRRKFKGVT 51

[Close]

BLAST of HO065824 vs. TAIR peptide
Match: AT5G46430.1 (| Symbols: | Ribosomal protein L32e | chr5:18833429-18834409 FORWARD LENGTH=133)

HSP 1 Score: 65.4698 bits (158), Expect = 7.772e-12
Identity = 33/51 (64.71%), Postives = 36/51 (70.59%), Query Frame = -1

Query:   19 MAVPLLTXXXXXXXXXXXXRPQSDRKVSVKPSWRRPKGIDPRVRRKFKGCT 171
            MAVPLLT            RPQSDR+++VK SWRRPKGID RVRRKFKG T
Sbjct:    1 MAVPLLTKKVVKKRSAKFIRPQSDRRITVKESWRRPKGIDSRVRRKFKGVT 51

[Close]

BLAST of HO065824 vs. TAIR peptide
Match: AT4G18100.1 (| Symbols: | Ribosomal protein L32e | chr4:10035715-10036475 REVERSE LENGTH=133)

HSP 1 Score: 65.4698 bits (158), Expect = 7.772e-12
Identity = 33/51 (64.71%), Postives = 36/51 (70.59%), Query Frame = -1

Query:   19 MAVPLLTXXXXXXXXXXXXRPQSDRKVSVKPSWRRPKGIDPRVRRKFKGCT 171
            MAVPLLT            RPQSDR+++VK SWRRPKGID RVRRKFKG T
Sbjct:    1 MAVPLLTKKVVKKRSAKFIRPQSDRRITVKESWRRPKGIDSRVRRKFKGVT 51

The following BLAST results are available for this feature:

BLAST of HO065824 vs. SwissProt
BLAST of HO065824 vs. TrEMBL
BLAST of HO065824 vs. TAIR peptide

BLAST of HO065824 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Swissprot)
Total hits: 2


Match Name	E-value	Identity	Description
RL322_ARATH	9.924e-11	64.71	60S ribosomal protein L32-2 OS=Arabidopsis thalian...	[more]
RL321_ARATH	9.924e-11	64.71	60S ribosomal protein L32-1 OS=Arabidopsis thalian...	[more]

BLAST of HO065824 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
Q0J4B8_ORYSJ	9.539e-12	56.52	Os08g0524600 protein (Fragment) OS=Oryza sativa su...	[more]
C6SZ81_SOYBN	9.539e-12	70.59	Putative uncharacterized protein OS=Glycine max PE...	[more]
C6SXB4_SOYBN	9.539e-12	70.59	Putative uncharacterized protein OS=Glycine max PE...	[more]
B9I117_POPTR	2.775e-11	70.59	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
A9PBE9_POPTR	6.183e-11	68.63	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
B9GQP4_POPTR	8.075e-11	66.67	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
A9PDT0_POPTR	8.075e-11	66.67	Putative uncharacterized protein OS=Populus tricho...	[more]
Q9XFE3_ORYSA	1.055e-10	62.50	60S ribosomal protein (Fragment) OS=Oryza sativa P...	[more]
Q0J0R0_ORYSJ	1.055e-10	62.50	Os09g0501700 protein (Fragment) OS=Oryza sativa su...	[more]
B9G4E8_ORYSJ	1.055e-10	62.50	Putative uncharacterized protein OS=Oryza sativa s...	[more]

BLAST of HO065824 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide)
Total hits: 3


Match Name	E-value	Identity	Description
AT5G46430.2	7.772e-12	64.71	\| Symbols: \| Ribosomal protein L32e \| chr5:188334...	[more]
AT5G46430.1	7.772e-12	64.71	\| Symbols: \| Ribosomal protein L32e \| chr5:188334...	[more]
AT4G18100.1	7.772e-12	64.71	\| Symbols: \| Ribosomal protein L32e \| chr4:100357...	[more]

InterPro

Analysis Name: InterProScan analysis for Cicer arietinum unigene v1
Date Performed: 2010-12-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001515	Ribosomal protein L32e	PANTHER	PTHR23413:SF1	60S RIBOSOMAL PROTEIN L32	coord: 51..85 score: 4.7
IPR001515	Ribosomal protein L32e	PFAM	PF01655	Ribosomal_L32e	coord: 48..86 score: 5.3
IPR001515	Ribosomal protein L32e	SUPERFAMILY	52042	Ribosomal protein L32e	coord: 21..82 score: 1.4
None	No IPR available	PANTHER	PTHR23413	60S RIBOSOMAL PROTEIN L32 AND DNA-DIRECTED RNA POLYMERASE II, SUBUNIT N	coord: 51..85 score: 4.7
None	No IPR available	SEG	seg	seg	coord: 41..53 score
None	No IPR available	SEG	seg	seg	coord: 1..18 score: NAcoord: 35..47 score

Properties


Property Name	Value
Singlet in analysis	Cicer arietinum unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>HO065824 ID=HO065824; Name=HO065824; organism=Cicer arietinum; type=EST; length=270bp
TAGCGTGGTCGCGGCCGAGGTACATCCTTTGAACTTTCTCCTTACACGGG
GATCAATACCCTTTGGCCTGCGCCAGCTTGGCTTAACAGAAACTTTCCTG
TCACTTTGAGGTCTCTTGAAGTGCTTGACACGCTTCTTCACAATTTTCTT
CGTTAGCAGAGGAACAGCCATCTTCTCTCTGCTTGAATTGAAACGGTGTT
CGTGAGATGTGAATAAGCAACGAGGCACTGAGGAGAAGTCCCCGCGTACC
TGCCCGGGCGGCCGCTCGAA

Annotated Terms

The following terms have been associated with this EST:

Vocabulary: **Molecular Function**
Term	Definition
GO:0003735	structural constituent of ribosome

Vocabulary: **Cellular Component**
Term	Definition
GO:0005622	intracellular anatomical structure
GO:0005840	ribosome

Vocabulary: **Biological Process**
Term	Definition
GO:0006412	translation

Vocabulary: **INTERPRO**
Term	Definition
IPR001515	Ribosomal_eL32

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