HO065314

EST Overview


Name	HO065314
Unique Name	HO065314
Type	EST
Organism	Cicer arietinum (chickpea)
Sequence length	261

Libraries


Library Name	Type
LIBEST_026412 Chickpea drought stressed cDNA SSH library AS1	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Cicer arietinum unigene v1	Cicer arietinum unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Cicer arietinum unigene v1	2010-12-27
Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide	2011-02-03
Homology Analysis: Cicer arietinum unigene v1 vs Swissprot	2010-12-28
Homology Analysis: Cicer arietinum unigene v1 vs Trembl	2010-12-28
InterProScan analysis for Cicer arietinum unigene v1	2010-12-29

Homology

[Close]

BLAST of HO065314 vs. SwissProt
Match: IF5A_SENVE (Eukaryotic translation initiation factor 5A OS=Senecio vernalis GN=EIFSV1 PE=2 SV=1)

HSP 1 Score: 56.225 bits (134), Expect = 6.103e-8
Identity = 28/48 (58.33%), Postives = 31/48 (64.58%), Query Frame = -2

Query:  114 TKGDLKLPSXETLLSQIKDGLGEGKXXXXSXXXTXGEEQICGLXDRVP 257
            TK DLKLP+ + LL+QIKDG GEGK    S     GEEQIC L D  P
Sbjct:  111 TKDDLKLPTDDALLTQIKDGFGEGKDLVVSVMSAMGEEQICALKDIGP 158

[Close]

BLAST of HO065314 vs. SwissProt
Match: IF5A5_SOLTU (Eukaryotic translation initiation factor 5A-5 OS=Solanum tuberosum GN=EIF5A5 PE=2 SV=1)

HSP 1 Score: 55.8398 bits (133), Expect = 7.971e-8
Identity = 26/48 (54.17%), Postives = 32/48 (66.67%), Query Frame = -2

Query:  114 TKGDLKLPSXETLLSQIKDGLGEGKXXXXSXXXTXGEEQICGLXDRVP 257
            TK DL+LP+ +TLL+Q+KDG  EGK    S     GEEQICG+ D  P
Sbjct:  111 TKDDLRLPTDDTLLAQVKDGFAEGKDLVLSVMSAMGEEQICGIKDIGP 158

[Close]

BLAST of HO065314 vs. SwissProt
Match: IF5A1_SOLLC (Eukaryotic translation initiation factor 5A-1 OS=Solanum lycopersicum PE=2 SV=1)

HSP 1 Score: 55.8398 bits (133), Expect = 7.971e-8
Identity = 26/48 (54.17%), Postives = 32/48 (66.67%), Query Frame = -2

Query:  114 TKGDLKLPSXETLLSQIKDGLGEGKXXXXSXXXTXGEEQICGLXDRVP 257
            TK DL+LP+ +TLL+Q+KDG  EGK    S     GEEQICG+ D  P
Sbjct:  111 TKDDLRLPTDDTLLAQVKDGFAEGKDLVLSVMSAMGEEQICGIKDIGP 158

[Close]

BLAST of HO065314 vs. SwissProt
Match: IF5A1_MEDSA (Eukaryotic translation initiation factor 5A-1 OS=Medicago sativa PE=2 SV=1)

HSP 1 Score: 53.9138 bits (128), Expect = 3.029e-7
Identity = 26/45 (57.78%), Postives = 30/45 (66.67%), Query Frame = -2

Query:  123 TKGDLKLPSXETLLSQIKDGLGEGKXXXXSXXXTXGEEQICGLXD 257
            TK DLKLP+ ++LL+QIKDG  EGK    S     GEEQIC L D
Sbjct:  112 TKDDLKLPTDDSLLTQIKDGFAEGKDLVVSVMSAMGEEQICALKD 156

[Close]

BLAST of HO065314 vs. SwissProt
Match: IF5A_MANES (Eukaryotic translation initiation factor 5A OS=Manihot esculenta PE=2 SV=2)

HSP 1 Score: 53.5286 bits (127), Expect = 3.956e-7
Identity = 27/48 (56.25%), Postives = 30/48 (62.50%), Query Frame = -2

Query:  114 TKGDLKLPSXETLLSQIKDGLGEGKXXXXSXXXTXGEEQICGLXDRVP 257
            TK DL+LP+ E LLSQIKDG  EGK    S     GEE+IC L D  P
Sbjct:  111 TKDDLRLPTDENLLSQIKDGFAEGKDLVVSVMSAMGEERICSLKDIGP 158

[Close]

BLAST of HO065314 vs. SwissProt
Match: IF5A2_MEDSA (Eukaryotic translation initiation factor 5A-2 OS=Medicago sativa PE=2 SV=1)

HSP 1 Score: 53.1434 bits (126), Expect = 5.166e-7
Identity = 26/48 (54.17%), Postives = 31/48 (64.58%), Query Frame = -2

Query:  114 TKGDLKLPSXETLLSQIKDGLGEGKXXXXSXXXTXGEEQICGLXDRVP 257
            TK DLKLP+ ++LL+QIKDG  +GK    S     GEEQIC L D  P
Sbjct:  111 TKDDLKLPTDDSLLTQIKDGFADGKDLVVSVMSAMGEEQICALKDIGP 158

[Close]

BLAST of HO065314 vs. SwissProt
Match: IF5A2_NICPL (Eukaryotic translation initiation factor 5A-2 OS=Nicotiana plumbaginifolia GN=EIF-5A2 PE=2 SV=1)

HSP 1 Score: 52.7582 bits (125), Expect = 6.747e-7
Identity = 26/48 (54.17%), Postives = 30/48 (62.50%), Query Frame = -2

Query:  114 TKGDLKLPSXETLLSQIKDGLGEGKXXXXSXXXTXGEEQICGLXDRVP 257
            TK DL+LP+ + LL+QIKDG  EGK    S     GEEQIC L D  P
Sbjct:  111 TKDDLRLPTDDNLLTQIKDGFAEGKDLVVSVMSAMGEEQICALKDIGP 158

[Close]

BLAST of HO065314 vs. TrEMBL
Match: B7FM94_MEDTR (Putative uncharacterized protein OS=Medicago truncatula PE=2 SV=1)

HSP 1 Score: 65.0846 bits (157), Expect = 2.637e-9
Identity = 33/49 (67.35%), Postives = 34/49 (69.39%), Query Frame = -2

Query:  114 GTKGDLKLPSXETLLSQIKDGLGEGKXXXXSXXXTXGEEQICGLXDRVP 260
            GTK DLKLP+ ETLLSQIKDG GEGK    S     GEEQICGL D  P
Sbjct:  110 GTKDDLKLPTDETLLSQIKDGFGEGKDLIVSVMSAMGEEQICGLKDIGP 158

[Close]

BLAST of HO065314 vs. TrEMBL
Match: Q6PQ38_9CARY (Translation initiation factor 5A OS=Tamarix androssowii PE=2 SV=1)

HSP 1 Score: 59.3066 bits (142), Expect = 1.447e-7
Identity = 30/49 (61.22%), Postives = 32/49 (65.31%), Query Frame = -2

Query:  114 GTKGDLKLPSXETLLSQIKDGLGEGKXXXXSXXXTXGEEQICGLXDRVP 260
            GTK DL+LPS ETLL+QIKDG  EGK    S     GEEQIC L D  P
Sbjct:  110 GTKDDLRLPSDETLLTQIKDGFAEGKDLIVSVMCAMGEEQICALKDIGP 158

[Close]

BLAST of HO065314 vs. TrEMBL
Match: C6ZHS3_SOYBN (Eukaryotic translation initiation factor 5A1 OS=Glycine max PE=2 SV=1)

HSP 1 Score: 57.7658 bits (138), Expect = 4.211e-7
Identity = 29/49 (59.18%), Postives = 32/49 (65.31%), Query Frame = -2

Query:  114 GTKGDLKLPSXETLLSQIKDGLGEGKXXXXSXXXTXGEEQICGLXDRVP 260
            GTK DL+LP+ E LLSQIKDG  EGK    S   + GEEQIC L D  P
Sbjct:  110 GTKDDLRLPTDENLLSQIKDGFAEGKDLVVSVMSSMGEEQICALKDIGP 158

[Close]

BLAST of HO065314 vs. TrEMBL
Match: C6SY82_SOYBN (Putative uncharacterized protein OS=Glycine max PE=2 SV=1)

HSP 1 Score: 57.7658 bits (138), Expect = 4.211e-7
Identity = 29/49 (59.18%), Postives = 32/49 (65.31%), Query Frame = -2

Query:  114 GTKGDLKLPSXETLLSQIKDGLGEGKXXXXSXXXTXGEEQICGLXDRVP 260
            GTK DL+LP+ E LLSQIKDG  EGK    S   + GEEQIC L D  P
Sbjct:  110 GTKDDLRLPTDENLLSQIKDGFAEGKDLVVSVMSSMGEEQICALKDIGP 158

[Close]

BLAST of HO065314 vs. TrEMBL
Match: C7SCX8_POPDE (eIF5A3 OS=Populus deltoides PE=2 SV=1)

HSP 1 Score: 56.9954 bits (136), Expect = 7.182e-7
Identity = 28/48 (58.33%), Postives = 33/48 (68.75%), Query Frame = -2

Query:  114 TKGDLKLPSXETLLSQIKDGLGEGKXXXXSXXXTXGEEQICGLXDRVP 257
            TK DL+LP+ E+LLSQIKDG GEGK    +   + GEEQIC L D  P
Sbjct:  111 TKDDLRLPTDESLLSQIKDGFGEGKDLVVTVMSSMGEEQICALKDVGP 158

[Close]

BLAST of HO065314 vs. TrEMBL
Match: C6T3J1_SOYBN (Putative uncharacterized protein OS=Glycine max PE=4 SV=1)

HSP 1 Score: 56.6102 bits (135), Expect = 9.380e-7
Identity = 28/49 (57.14%), Postives = 32/49 (65.31%), Query Frame = -2

Query:  114 GTKGDLKLPSXETLLSQIKDGLGEGKXXXXSXXXTXGEEQICGLXDRVP 260
            GTK DL+LP+ E LL+QIKDG  EGK    S   + GEEQIC L D  P
Sbjct:   69 GTKDDLRLPTDENLLTQIKDGFAEGKDLVVSVMSSMGEEQICALKDIGP 117

[Close]

BLAST of HO065314 vs. TrEMBL
Match: C6SVG2_SOYBN (Putative uncharacterized protein OS=Glycine max PE=2 SV=1)

HSP 1 Score: 56.6102 bits (135), Expect = 9.380e-7
Identity = 28/49 (57.14%), Postives = 32/49 (65.31%), Query Frame = -2

Query:  114 GTKGDLKLPSXETLLSQIKDGLGEGKXXXXSXXXTXGEEQICGLXDRVP 260
            GTK DL+LP+ E LL+QIKDG  EGK    S   + GEEQIC L D  P
Sbjct:  110 GTKDDLRLPTDENLLTQIKDGFAEGKDLVVSVMSSMGEEQICALKDIGP 158

[Close]

BLAST of HO065314 vs. TAIR peptide
Match: AT1G69410.1 (| Symbols: ATELF5A-3, ELF5A-3 | eukaryotic elongation factor 5A-3 | chr1:26089301-26090194 FORWARD LENGTH=158)

HSP 1 Score: 48.9062 bits (115), Expect = 7.615e-7
Identity = 24/48 (50.00%), Postives = 30/48 (62.50%), Query Frame = -2

Query:  114 TKGDLKLPSXETLLSQIKDGLGEGKXXXXSXXXTXGEEQICGLXDRVP 257
            TK DLKLP+ E LL+Q+K+G  EGK    S     GEEQ+C L +  P
Sbjct:  110 TKDDLKLPTDEALLTQLKNGFEEGKDIVVSVMSAMGEEQMCALKEVGP 157

The following BLAST results are available for this feature:

BLAST of HO065314 vs. SwissProt
BLAST of HO065314 vs. TrEMBL
BLAST of HO065314 vs. TAIR peptide

BLAST of HO065314 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Swissprot)
Total hits: 7


Match Name	E-value	Identity	Description
IF5A_SENVE	6.103e-8	58.33	Eukaryotic translation initiation factor 5A OS=Sen...	[more]
IF5A5_SOLTU	7.971e-8	54.17	Eukaryotic translation initiation factor 5A-5 OS=S...	[more]
IF5A1_SOLLC	7.971e-8	54.17	Eukaryotic translation initiation factor 5A-1 OS=S...	[more]
IF5A1_MEDSA	3.029e-7	57.78	Eukaryotic translation initiation factor 5A-1 OS=M...	[more]
IF5A_MANES	3.956e-7	56.25	Eukaryotic translation initiation factor 5A OS=Man...	[more]
IF5A2_MEDSA	5.166e-7	54.17	Eukaryotic translation initiation factor 5A-2 OS=M...	[more]
IF5A2_NICPL	6.747e-7	54.17	Eukaryotic translation initiation factor 5A-2 OS=N...	[more]

BLAST of HO065314 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Trembl)
Total hits: 7


Match Name	E-value	Identity	Description
B7FM94_MEDTR	2.637e-9	67.35	Putative uncharacterized protein OS=Medicago trunc...	[more]
Q6PQ38_9CARY	1.447e-7	61.22	Translation initiation factor 5A OS=Tamarix andros...	[more]
C6ZHS3_SOYBN	4.211e-7	59.18	Eukaryotic translation initiation factor 5A1 OS=Gl...	[more]
C6SY82_SOYBN	4.211e-7	59.18	Putative uncharacterized protein OS=Glycine max PE...	[more]
C7SCX8_POPDE	7.182e-7	58.33	eIF5A3 OS=Populus deltoides PE=2 SV=1	[more]
C6T3J1_SOYBN	9.380e-7	57.14	Putative uncharacterized protein OS=Glycine max PE...	[more]
C6SVG2_SOYBN	9.380e-7	57.14	Putative uncharacterized protein OS=Glycine max PE...	[more]

BLAST of HO065314 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide)
Total hits: 1


Match Name	E-value	Identity	Description
AT1G69410.1	7.615e-7	50.00	\| Symbols: ATELF5A-3, ELF5A-3 \| eukaryotic elongat...	[more]

InterPro

Analysis Name: InterProScan analysis for Cicer arietinum unigene v1
Date Performed: 2010-12-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	SEG	seg	seg	coord: 33..53 score
None	No IPR available	SEG	seg	seg	coord: 47..60 score
None	No IPR available	SEG	seg	seg	coord: 14..29 score: NAcoord: 32..36 score
None	No IPR available	SEG	seg	seg	coord: 25..34 score
None	No IPR available	SEG	seg	seg	coord: 27..34 score
IPR001884	Translation elongation factor IF5A	PANTHER	PTHR11673	TRANSLATION INITIATION FACTOR 5A-RELATED	coord: 2..50 score: 8.7
IPR012340	Nucleic acid-binding, OB-fold	GENE3D	2.40.50.140	no description	coord: 2..44 score: 8.3

Properties


Property Name	Value
Singlet in analysis	Cicer arietinum unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>HO065314 ID=HO065314; Name=HO065314; organism=Cicer arietinum; type=EST; length=261bp
ATCTGGTCAGAATTTCTCANGGCTTAGGTTTATATCTTTACATGACANAC
TCCCNAATATGTAANGATNCCNTCCCCCTTCCAANGGNCATTTANGGGGC
NCTANATGTNACCTGGGACCCTGTCCNTAAGGCCACAAATCTGCTCCTCN
CCCNTGGTNNANNTANCACTCNCNATNANATNTTTACCCTCCCCCAATCC
ATCCTTGATCTGGGAAAGCAGAGTTTCANCGGANGGAAGCTTGAGGTCAC
CCTTAGTACCT

Annotated Terms

The following terms have been associated with this EST:

Vocabulary: **Molecular Function**
Term	Definition
GO:0003723	RNA binding
GO:0003746	translation elongation factor activity
GO:0043022	ribosome binding

Vocabulary: **Biological Process**
Term	Definition
GO:0006452	translational frameshifting
GO:0008612	peptidyl-lysine modification to peptidyl-hypusine
GO:0045901	positive regulation of translational elongation
GO:0045905	positive regulation of translational termination

Vocabulary: **INTERPRO**
Term	Definition
IPR001884	IF5A-like
IPR012340	NA-bd_OB-fold

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