HO067796

EST Overview


Name	HO067796
Unique Name	HO067796
Type	EST
Organism	Cicer arietinum (chickpea)
Sequence length	436

Libraries


Library Name	Type
LIBEST_026415 Chickpea drought stressed cDNA SSH library BULK2	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Cicer arietinum unigene v1	Cicer arietinum unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Cicer arietinum unigene v1	2010-12-27
Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide	2011-02-03
Homology Analysis: Cicer arietinum unigene v1 vs Swissprot	2010-12-28
Homology Analysis: Cicer arietinum unigene v1 vs Trembl	2010-12-28
InterProScan analysis for Cicer arietinum unigene v1	2010-12-29

Homology

[Close]

BLAST of HO067796 vs. SwissProt
Match: RL14_PEA (Probable 60S ribosomal protein L14 OS=Pisum sativum PE=2 SV=1)

HSP 1 Score: 109.768 bits (273), Expect = 5.028e-24
Identity = 55/61 (90.16%), Postives = 57/61 (93.44%), Query Frame = 2

Query:    2 VPKKKDLIKAMEAADVKGKWEKSSWGRKLIVRKRRAALNDFDRFKIMLAKIKRAAVVRQEL 184
            VPKKKDLIKA+EAADVK KW KSSWGRKLIV+K RAALNDFDRFKIMLAKIKRAA VRQEL
Sbjct:   65 VPKKKDLIKALEAADVKNKWAKSSWGRKLIVKKTRAALNDFDRFKIMLAKIKRAAGVRQEL 125

[Close]

BLAST of HO067796 vs. SwissProt
Match: RL141_ARATH (60S ribosomal protein L14-1 OS=Arabidopsis thaliana GN=RPL14A PE=2 SV=1)

HSP 1 Score: 108.997 bits (271), Expect = 8.577e-24
Identity = 54/61 (88.52%), Postives = 57/61 (93.44%), Query Frame = 2

Query:    2 VPKKKDLIKAMEAADVKGKWEKSSWGRKLIVRKRRAALNDFDRFKIMLAKIKRAAVVRQEL 184
            VPKKK LI+AME ADVK KWEKSSWGRKLIV+KRRAALNDFDRFKIMLAKIKRA +VRQEL
Sbjct:   65 VPKKKVLIEAMEKADVKNKWEKSSWGRKLIVQKRRAALNDFDRFKIMLAKIKRAGIVRQEL 125

[Close]

BLAST of HO067796 vs. SwissProt
Match: RL142_ARATH (60S ribosomal protein L14-2 OS=Arabidopsis thaliana GN=RPL14B PE=2 SV=1)

HSP 1 Score: 106.301 bits (264), Expect = 5.559e-23
Identity = 53/61 (86.89%), Postives = 56/61 (91.80%), Query Frame = 2

Query:    2 VPKKKDLIKAMEAADVKGKWEKSSWGRKLIVRKRRAALNDFDRFKIMLAKIKRAAVVRQEL 184
            VPKKK LI+AME ADVK KWEKSSWGRKLIV+KRRA LNDFDRFKIMLAKIK+A VVRQEL
Sbjct:   65 VPKKKALIEAMEKADVKNKWEKSSWGRKLIVQKRRANLNDFDRFKIMLAKIKKAGVVRQEL 125

[Close]

BLAST of HO067796 vs. SwissProt
Match: RL14_RAT (60S ribosomal protein L14 OS=Rattus norvegicus GN=Rpl14 PE=1 SV=3)

HSP 1 Score: 56.6102 bits (135), Expect = 5.052e-8
Identity = 24/59 (40.68%), Postives = 39/59 (66.10%), Query Frame = 2

Query:    8 KKKDLIKAMEAADVKGKWEKSSWGRKLIVRKRRAALNDFDRFKIMLAKIKRAAVVRQEL 184
            ++K + KA E AD+  KW  + W +K+  R+R+A + DFDRFK+M AK  R  +++ E+
Sbjct:   69 RQKYVRKAWEKADINTKWAATRWAKKIDARERKAKMTDFDRFKVMKAKKMRNRIIKTEV 127

[Close]

BLAST of HO067796 vs. SwissProt
Match: RL14_MOUSE (60S ribosomal protein L14 OS=Mus musculus GN=Rpl14 PE=2 SV=3)

HSP 1 Score: 56.6102 bits (135), Expect = 5.052e-8
Identity = 24/59 (40.68%), Postives = 39/59 (66.10%), Query Frame = 2

Query:    8 KKKDLIKAMEAADVKGKWEKSSWGRKLIVRKRRAALNDFDRFKIMLAKIKRAAVVRQEL 184
            ++K + KA E AD+  KW  + W +K+  R+R+A + DFDRFK+M AK  R  +++ E+
Sbjct:   69 RQKYVRKAWEKADINTKWAATRWAKKIDARERKAKMTDFDRFKVMKAKKMRNRIIKTEV 127

[Close]

BLAST of HO067796 vs. SwissProt
Match: RL14_HUMAN (60S ribosomal protein L14 OS=Homo sapiens GN=RPL14 PE=1 SV=4)

HSP 1 Score: 53.5286 bits (127), Expect = 4.276e-7
Identity = 22/58 (37.93%), Postives = 38/58 (65.52%), Query Frame = 2

Query:   11 KKDLIKAMEAADVKGKWEKSSWGRKLIVRKRRAALNDFDRFKIMLAKIKRAAVVRQEL 184
            +K + +A + AD+  KW  + W +K+  R+R+A + DFDRFK+M AK  R  +++ E+
Sbjct:   70 QKYVRQAWQKADINTKWAATRWAKKIEARERKAKMTDFDRFKVMKAKKMRNRIIKNEV 127

[Close]

BLAST of HO067796 vs. SwissProt
Match: RL14_PIG (60S ribosomal protein L14 OS=Sus scrofa GN=RPL14 PE=2 SV=1)

HSP 1 Score: 52.7582 bits (125), Expect = 7.294e-7
Identity = 21/59 (35.59%), Postives = 39/59 (66.10%), Query Frame = 2

Query:    8 KKKDLIKAMEAADVKGKWEKSSWGRKLIVRKRRAALNDFDRFKIMLAKIKRAAVVRQEL 184
            ++K + KA E AD+  KW  + W +K+  R+++A + DFDR+K+M A+  R  +++ E+
Sbjct:   69 RQKYVRKAWEKADINAKWAATRWAKKIEAREKKAKMTDFDRYKVMKARKMRNRLIKLEV 127

[Close]

BLAST of HO067796 vs. SwissProt
Match: RL14_GRIJA (60S ribosomal protein L14 OS=Griffithsia japonica GN=RPL14 PE=2 SV=1)

HSP 1 Score: 52.7582 bits (125), Expect = 7.294e-7
Identity = 26/68 (38.24%), Postives = 41/68 (60.29%), Query Frame = 2

Query:    8 KKKDLIKAMEAADVKGKWEK---------SSWGRKLIVRKRRAALNDFDRFKIMLAKIKRAAVVRQEL 184
            K  D+ +    A+VK K+           S WG+KL  R++RAAL+DF RFK+M+A++K++  +  EL
Sbjct:   61 KVDDIARGAPVAEVKSKYTAAKVDETFAASGWGKKLAKREKRAALDDFGRFKVMVARMKKSKAINAEL 128

[Close]

BLAST of HO067796 vs. SwissProt
Match: RL14_BOVIN (60S ribosomal protein L14 OS=Bos taurus GN=RPL14 PE=2 SV=3)

HSP 1 Score: 52.373 bits (124), Expect = 9.527e-7
Identity = 21/59 (35.59%), Postives = 39/59 (66.10%), Query Frame = 2

Query:    8 KKKDLIKAMEAADVKGKWEKSSWGRKLIVRKRRAALNDFDRFKIMLAKIKRAAVVRQEL 184
            ++K + KA E AD+  KW  + W +K+  R+++A + DFDR+K+M A+  R  +++ E+
Sbjct:   69 RQKYVRKAWEKADINAKWAATRWAKKIEAREKKAKMTDFDRYKVMKARKMRNRLIKIEV 127

[Close]

BLAST of HO067796 vs. TrEMBL
Match: B7FL54_MEDTR (Putative uncharacterized protein OS=Medicago truncatula PE=2 SV=1)

HSP 1 Score: 116.701 bits (291), Expect = 7.579e-25
Identity = 58/61 (95.08%), Postives = 59/61 (96.72%), Query Frame = 2

Query:    2 VPKKKDLIKAMEAADVKGKWEKSSWGRKLIVRKRRAALNDFDRFKIMLAKIKRAAVVRQEL 184
            VPKKKDLIKAMEAADVK KWEKSSWGRKLIV+KRRAALNDFDRFKIMLAKIKRA VVRQEL
Sbjct:   65 VPKKKDLIKAMEAADVKNKWEKSSWGRKLIVKKRRAALNDFDRFKIMLAKIKRAGVVRQEL 125

[Close]

BLAST of HO067796 vs. TrEMBL
Match: C6SXV0_SOYBN (Putative uncharacterized protein OS=Glycine max PE=2 SV=1)

HSP 1 Score: 115.546 bits (288), Expect = 1.688e-24
Identity = 57/61 (93.44%), Postives = 59/61 (96.72%), Query Frame = 2

Query:    2 VPKKKDLIKAMEAADVKGKWEKSSWGRKLIVRKRRAALNDFDRFKIMLAKIKRAAVVRQEL 184
            VPKKKDL+KAME ADVK KWEKSSWGRKLIVRKRRA+LNDFDRFKIMLAKIKRAAVVRQEL
Sbjct:   65 VPKKKDLVKAMEDADVKNKWEKSSWGRKLIVRKRRASLNDFDRFKIMLAKIKRAAVVRQEL 125

[Close]

BLAST of HO067796 vs. TrEMBL
Match: C6SY27_SOYBN (Putative uncharacterized protein OS=Glycine max PE=2 SV=1)

HSP 1 Score: 113.62 bits (283), Expect = 6.416e-24
Identity = 56/61 (91.80%), Postives = 59/61 (96.72%), Query Frame = 2

Query:    2 VPKKKDLIKAMEAADVKGKWEKSSWGRKLIVRKRRAALNDFDRFKIMLAKIKRAAVVRQEL 184
            VPKKKDL+KAME ADVK KWEKSSWGRKLIVRKRRA+LNDFDRFKIMLAKIK+AAVVRQEL
Sbjct:   65 VPKKKDLVKAMEDADVKTKWEKSSWGRKLIVRKRRASLNDFDRFKIMLAKIKKAAVVRQEL 125

[Close]

BLAST of HO067796 vs. TrEMBL
Match: D7L3N5_ARALY (Predicted protein OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_674786 PE=4 SV=1)

HSP 1 Score: 109.768 bits (273), Expect = 9.264e-23
Identity = 54/61 (88.52%), Postives = 57/61 (93.44%), Query Frame = 2

Query:    2 VPKKKDLIKAMEAADVKGKWEKSSWGRKLIVRKRRAALNDFDRFKIMLAKIKRAAVVRQEL 184
            VPKKK L++AME ADVK KWEKSSWGRKLIV+KRRAALNDFDRFKIMLAKIKRA VVRQEL
Sbjct:   65 VPKKKHLVEAMEKADVKNKWEKSSWGRKLIVQKRRAALNDFDRFKIMLAKIKRAGVVRQEL 125

[Close]

BLAST of HO067796 vs. TrEMBL
Match: Q3HVK7_SOLTU (Glycoprotein-like protein OS=Solanum tuberosum PE=2 SV=1)

HSP 1 Score: 106.301 bits (264), Expect = 1.024e-21
Identity = 52/61 (85.25%), Postives = 56/61 (91.80%), Query Frame = 2

Query:    2 VPKKKDLIKAMEAADVKGKWEKSSWGRKLIVRKRRAALNDFDRFKIMLAKIKRAAVVRQEL 184
            +PKKK LI AMEAADVK KWE SSWGRKLIV+KRRA+LNDFDRFK+MLAKIKRA VVRQEL
Sbjct:   65 IPKKKTLIAAMEAADVKTKWESSSWGRKLIVQKRRASLNDFDRFKLMLAKIKRAGVVRQEL 125

[Close]

BLAST of HO067796 vs. TrEMBL
Match: B9DHP0_ARATH (AT4G27090 protein OS=Arabidopsis thaliana GN=AT4G27090 PE=2 SV=1)

HSP 1 Score: 106.301 bits (264), Expect = 1.024e-21
Identity = 53/61 (86.89%), Postives = 56/61 (91.80%), Query Frame = 2

Query:    2 VPKKKDLIKAMEAADVKGKWEKSSWGRKLIVRKRRAALNDFDRFKIMLAKIKRAAVVRQEL 184
            VPKKK LI+AME ADVK KWEKSSWGRKLIV+KRRA LNDFDRFKIMLAKIK+A VVRQEL
Sbjct:   65 VPKKKALIEAMEKADVKNKWEKSSWGRKLIVQKRRANLNDFDRFKIMLAKIKKAGVVRQEL 125

[Close]

BLAST of HO067796 vs. TrEMBL
Match: D7T502_VITVI (Whole genome shotgun sequence of line PN40024, scaffold_118.assembly12x (Fragment) OS=Vitis vinifera GN=VIT_00012876001 PE=4 SV=1)

HSP 1 Score: 105.145 bits (261), Expect = 2.282e-21
Identity = 51/61 (83.61%), Postives = 55/61 (90.16%), Query Frame = 2

Query:    2 VPKKKDLIKAMEAADVKGKWEKSSWGRKLIVRKRRAALNDFDRFKIMLAKIKRAAVVRQEL 184
            VPKKK LI+AM AADVK KWE SSWGRKLIV+KRRA+LNDFDRFKIMLAKIKR  V+RQEL
Sbjct:  110 VPKKKTLIEAMNAADVKNKWENSSWGRKLIVQKRRASLNDFDRFKIMLAKIKRGGVIRQEL 170

[Close]

BLAST of HO067796 vs. TrEMBL
Match: D7MEJ2_ARALY (60S ribosomal protein L14 OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_492088 PE=4 SV=1)

HSP 1 Score: 105.145 bits (261), Expect = 2.282e-21
Identity = 52/61 (85.25%), Postives = 56/61 (91.80%), Query Frame = 2

Query:    2 VPKKKDLIKAMEAADVKGKWEKSSWGRKLIVRKRRAALNDFDRFKIMLAKIKRAAVVRQEL 184
            VPKKK LI+AM+ ADVK KWEKSSWGRKLIV+KRRA LNDFDRFKIMLAKIK+A VVRQEL
Sbjct:   65 VPKKKALIEAMDKADVKNKWEKSSWGRKLIVQKRRANLNDFDRFKIMLAKIKKAGVVRQEL 125

[Close]

BLAST of HO067796 vs. TrEMBL
Match: B9SS61_RICCO (60S ribosomal protein L14, putative OS=Ricinus communis GN=RCOM_1169110 PE=4 SV=1)

HSP 1 Score: 105.145 bits (261), Expect = 2.282e-21
Identity = 50/61 (81.97%), Postives = 56/61 (91.80%), Query Frame = 2

Query:    2 VPKKKDLIKAMEAADVKGKWEKSSWGRKLIVRKRRAALNDFDRFKIMLAKIKRAAVVRQEL 184
            VPKKK+LI+AME ADVK KWE SSWGRKLIV+KRRAALNDFDRFK+MLAKIKR  ++RQEL
Sbjct:   65 VPKKKNLIEAMEKADVKNKWESSSWGRKLIVQKRRAALNDFDRFKLMLAKIKRGGLIRQEL 125

[Close]

BLAST of HO067796 vs. TrEMBL
Match: D7SZI9_VITVI (Whole genome shotgun sequence of line PN40024, scaffold_49.assembly12x (Fragment) OS=Vitis vinifera GN=VIT_00027844001 PE=4 SV=1)

HSP 1 Score: 104.76 bits (260), Expect = 2.980e-21
Identity = 51/61 (83.61%), Postives = 55/61 (90.16%), Query Frame = 2

Query:    2 VPKKKDLIKAMEAADVKGKWEKSSWGRKLIVRKRRAALNDFDRFKIMLAKIKRAAVVRQEL 184
            VPKKK LI+AM AADVK KWE SSWGRKLIV+KRRA+LNDFDRFKIMLAKIKR  V+RQEL
Sbjct:  151 VPKKKTLIEAMTAADVKNKWENSSWGRKLIVQKRRASLNDFDRFKIMLAKIKRGGVIRQEL 211

[Close]

BLAST of HO067796 vs. TAIR peptide
Match: AT2G20450.1 (| Symbols: | Ribosomal protein L14 | chr2:8813923-8815071 FORWARD LENGTH=134)

HSP 1 Score: 108.997 bits (271), Expect = 1.043e-24
Identity = 54/61 (88.52%), Postives = 57/61 (93.44%), Query Frame = 2

Query:    2 VPKKKDLIKAMEAADVKGKWEKSSWGRKLIVRKRRAALNDFDRFKIMLAKIKRAAVVRQEL 184
            VPKKK LI+AME ADVK KWEKSSWGRKLIV+KRRAALNDFDRFKIMLAKIKRA +VRQEL
Sbjct:   65 VPKKKVLIEAMEKADVKNKWEKSSWGRKLIVQKRRAALNDFDRFKIMLAKIKRAGIVRQEL 125

[Close]

BLAST of HO067796 vs. TAIR peptide
Match: AT4G27090.1 (| Symbols: | Ribosomal protein L14 | chr4:13594104-13595187 REVERSE LENGTH=134)

HSP 1 Score: 106.301 bits (264), Expect = 6.761e-24
Identity = 53/61 (86.89%), Postives = 56/61 (91.80%), Query Frame = 2

Query:    2 VPKKKDLIKAMEAADVKGKWEKSSWGRKLIVRKRRAALNDFDRFKIMLAKIKRAAVVRQEL 184
            VPKKK LI+AME ADVK KWEKSSWGRKLIV+KRRA LNDFDRFKIMLAKIK+A VVRQEL
Sbjct:   65 VPKKKALIEAMEKADVKNKWEKSSWGRKLIVQKRRANLNDFDRFKIMLAKIKKAGVVRQEL 125

The following BLAST results are available for this feature:

BLAST of HO067796 vs. SwissProt
BLAST of HO067796 vs. TrEMBL
BLAST of HO067796 vs. TAIR peptide

BLAST of HO067796 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Swissprot)
Total hits: 9


Match Name	E-value	Identity	Description
RL14_PEA	5.028e-24	90.16	Probable 60S ribosomal protein L14 OS=Pisum sativu...	[more]
RL141_ARATH	8.577e-24	88.52	60S ribosomal protein L14-1 OS=Arabidopsis thalian...	[more]
RL142_ARATH	5.559e-23	86.89	60S ribosomal protein L14-2 OS=Arabidopsis thalian...	[more]
RL14_RAT	5.052e-8	40.68	60S ribosomal protein L14 OS=Rattus norvegicus GN=...	[more]
RL14_MOUSE	5.052e-8	40.68	60S ribosomal protein L14 OS=Mus musculus GN=Rpl14...	[more]
RL14_HUMAN	4.276e-7	37.93	60S ribosomal protein L14 OS=Homo sapiens GN=RPL14...	[more]
RL14_PIG	7.294e-7	35.59	60S ribosomal protein L14 OS=Sus scrofa GN=RPL14 P...	[more]
RL14_GRIJA	7.294e-7	38.24	60S ribosomal protein L14 OS=Griffithsia japonica ...	[more]
RL14_BOVIN	9.527e-7	35.59	60S ribosomal protein L14 OS=Bos taurus GN=RPL14 P...	[more]

BLAST of HO067796 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
B7FL54_MEDTR	7.579e-25	95.08	Putative uncharacterized protein OS=Medicago trunc...	[more]
C6SXV0_SOYBN	1.688e-24	93.44	Putative uncharacterized protein OS=Glycine max PE...	[more]
C6SY27_SOYBN	6.416e-24	91.80	Putative uncharacterized protein OS=Glycine max PE...	[more]
D7L3N5_ARALY	9.264e-23	88.52	Predicted protein OS=Arabidopsis lyrata subsp. lyr...	[more]
Q3HVK7_SOLTU	1.024e-21	85.25	Glycoprotein-like protein OS=Solanum tuberosum PE=...	[more]
B9DHP0_ARATH	1.024e-21	86.89	AT4G27090 protein OS=Arabidopsis thaliana GN=AT4G2...	[more]
D7T502_VITVI	2.282e-21	83.61	Whole genome shotgun sequence of line PN40024, sca...	[more]
D7MEJ2_ARALY	2.282e-21	85.25	60S ribosomal protein L14 OS=Arabidopsis lyrata su...	[more]
B9SS61_RICCO	2.282e-21	81.97	60S ribosomal protein L14, putative OS=Ricinus com...	[more]
D7SZI9_VITVI	2.980e-21	83.61	Whole genome shotgun sequence of line PN40024, sca...	[more]

BLAST of HO067796 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide)
Total hits: 2


Match Name	E-value	Identity	Description
AT2G20450.1	1.043e-24	88.52	\| Symbols: \| Ribosomal protein L14 \| chr2:8813923...	[more]
AT4G27090.1	6.761e-24	86.89	\| Symbols: \| Ribosomal protein L14 \| chr4:1359410...	[more]

InterPro

Analysis Name: InterProScan analysis for Cicer arietinum unigene v1
Date Performed: 2010-12-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002784	Ribosomal protein L14	PFAM	PF01929	Ribosomal_L14e	coord: 3..55 score: 2.2
None	No IPR available	PANTHER	PTHR11127	60S RIBOSOMAL PROTEIN L14	coord: 2..69 score: 1.1
None	No IPR available	SEG	seg	seg	coord: 6..27 score
None	No IPR available	SEG	seg	seg	coord: 7..20 score

Properties


Property Name	Value
Singlet in analysis	Cicer arietinum unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>HO067796 ID=HO067796; Name=HO067796; organism=Cicer arietinum; type=EST; length=436bp
AGTTCCTAAGAAGAAGGATCTCATCAAGGCCATGGAAGCTGCTGATGTGA
AGGGCAAGTGGGAGAAGAGCTCTTGGGGTAGAAAACTGATTGTCAGAAAG
AGAAGGGCAGCCCTCAATGATTTTGATAGGTTCAAGATTATGTTGGCAAA
GATTAAGAGGGCTGCTGTCGTTAGGCAAGAACTTGCTAAGCTGAAGAAGA
CTGCAGCTTAAGCATTTATTTTCAAATTTTGTTATCTATTTCCTTCCATT
TAGTACAACTTGGATTTTGGTTTTTTGTATCACTAAGTTTGGTAAATGTT
TCGAGAAATTTATATTTATGTTAAAGAGATTATATCAGTACTCAATTCTC
CATTGTTTTTGAATGCTTATTTTTGAATGTTGGTTTATTAGATAATCGAT
AAAGCAAGCTAGAGCTTGAAATTTTATTTGTGTTAC

Annotated Terms

The following terms have been associated with this EST:

Vocabulary: **Molecular Function**
Term	Definition
GO:0003735	structural constituent of ribosome

Vocabulary: **Cellular Component**
Term	Definition
GO:0005622	intracellular anatomical structure
GO:0005840	ribosome

Vocabulary: **Biological Process**
Term	Definition
GO:0006412	translation

Vocabulary: **INTERPRO**
Term	Definition
IPR002784	Ribosomal_eL14_dom

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