HO067833

EST Overview


Name	HO067833
Unique Name	HO067833
Type	EST
Organism	Cicer arietinum (chickpea)
Sequence length	604

Libraries


Library Name	Type
LIBEST_026415 Chickpea drought stressed cDNA SSH library BULK2	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Cicer arietinum unigene v1	Cicer arietinum unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Cicer arietinum unigene v1	2010-12-27
Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide	2011-02-03
Homology Analysis: Cicer arietinum unigene v1 vs Swissprot	2010-12-28
Homology Analysis: Cicer arietinum unigene v1 vs Trembl	2010-12-28
InterProScan analysis for Cicer arietinum unigene v1	2010-12-29

Homology

[Close]

BLAST of HO067833 vs. SwissProt
Match: AGD11_ARATH (Probable ADP-ribosylation factor GTPase-activating protein AGD11 OS=Arabidopsis thaliana GN=AGD11 PE=2 SV=1)

HSP 1 Score: 95.9005 bits (237), Expect = 1.680e-19
Identity = 47/121 (38.84%), Postives = 76/121 (62.81%), Query Frame = 3

Query:  141 KEKLKTRVVKNNCNPEWNEELTLSIRDVKTPIHLTVYDKATFSVDDKMGNADVDLKPYVECVQ-MKLSSLPNGCAIKRIIPNRTNCLAEESSCVWKNGSIVQEMILRLKNVESGELVVEIE 500
            ++ +KTRV+KNN NP WNE L LSI +   P+ + VYDK TFS DD MG A++D++P V   +  + SS+     +   + ++ N L  +   + ++G + Q++ LRL+NVE G L +++E
Sbjct:  259 QQSVKTRVIKNNLNPVWNETLMLSIPEPMPPLKVLVYDKDTFSTDDFMGEAEIDIQPLVSAAKAYETSSIKEPMQLGSWVASKENTLVSDGIILLEDGKVKQDISLRLQNVERGVLEIQLE 379

[Close]

BLAST of HO067833 vs. SwissProt
Match: AGD13_ARATH (Probable ADP-ribosylation factor GTPase-activating protein AGD13 OS=Arabidopsis thaliana GN=AGD13 PE=2 SV=1)

HSP 1 Score: 92.4337 bits (228), Expect = 1.857e-18
Identity = 48/123 (39.02%), Postives = 76/123 (61.79%), Query Frame = 3

Query:  141 KEKLKTRVVKNNCNPEWNEELTLSIRDVKTPIHLTVYDKATFSVDDKMGNADVDLKPYV-ECVQMKLSSLPNGCAIKRIIPNRTNCLAEESSCVWKNGSIVQEMILRLKNVESGELVVEIEWV 506
            K+KL+T V+ +N NP WN+EL LS+ +   P+ L VYD  TFS DD MG AD+D++P +   +      +     I + + +  N L ++S     +G + QE+ ++L+NVESGEL +E+EW+
Sbjct:  210 KQKLQTTVMNSNLNPVWNQELMLSVPESYGPVKLQVYDYDTFSADDIMGEADIDIQPLITSAMAFGDPEMFGDMQIGKWLKSHDNPLIDDSIINIVDGKVKQEVQIKLQNVESGELELEMEWL 332

[Close]

BLAST of HO067833 vs. SwissProt
Match: AGD12_ARATH (ADP-ribosylation factor GTPase-activating protein AGD12 OS=Arabidopsis thaliana GN=AGD12 PE=1 SV=1)

HSP 1 Score: 86.6557 bits (213), Expect = 1.019e-16
Identity = 46/123 (37.40%), Postives = 74/123 (60.16%), Query Frame = 3

Query:  141 KEKLKTRVVKNNCNPEWNEELTLSIRDVKTPIHLTVYDKATFSVDDKMGNADVDLKPYV-ECVQMKLSSLPNGCAIKRIIPNRTNCLAEESSCVWKNGSIVQEMILRLKNVESGELVVEIEWV 506
            ++K ++ VVK+N NP WNEEL LS+      + L V+D  TFS DD MG A++D++P +   +      +     I + + +  N L E+S     +G + QE+ ++L+NVESGEL +E+EW+
Sbjct:  211 QQKAQSTVVKSNLNPVWNEELMLSVPHNYGSVKLQVFDYDTFSADDIMGEAEIDIQPLITSAMAFGDPEMFGDMQIGKWLKSHDNALIEDSIINIADGKVKQEVQIKLQNVESGELELEMEWL 333

[Close]

BLAST of HO067833 vs. TrEMBL
Match: C6TCZ1_SOYBN (Putative uncharacterized protein OS=Glycine max PE=2 SV=1)

HSP 1 Score: 224.172 bits (570), Expect = 1.595e-59
Identity = 101/126 (80.16%), Postives = 116/126 (92.06%), Query Frame = 3

Query:  144 EKLKTRVVKNNCNPEWNEELTLSIRDVKTPIHLTVYDKATFSVDDKMGNADVDLKPYVECVQMKLSSLPNGCAIKRIIPNRTNCLAEESSCVWKNGSIVQEMILRLKNVESGELVVEIEWVDVPGC 521
            +KLKTRVVKNNCNP+WNEELTLS++DVKTPIHLTVYDK TFSVDDKMG A++DLKPYV+C QM L  LPNGC++KRI P+RTNCLAEESSC+W+NG IVQEM LRL+NVESGE++VEIEWVDV GC
Sbjct:   47 QKLKTRVVKNNCNPDWNEELTLSVKDVKTPIHLTVYDKDTFSVDDKMGEAEIDLKPYVQCKQMGLGKLPNGCSLKRIQPDRTNCLAEESSCIWQNGKIVQEMFLRLRNVESGEILVEIEWVDVVGC 172

HSP 2 Score: 30.4166 bits (67), Expect = 1.595e-59
Identity = 17/19 (89.47%), Postives = 17/19 (89.47%), Query Frame = 2

Query:   98 SMDNN-ILGLLKLRIKREV 151
            SMDNN ILGLLKLRIKR V
Sbjct:    8 SMDNNNILGLLKLRIKRGV 26

[Close]

BLAST of HO067833 vs. TrEMBL
Match: C6SXX9_SOYBN (Putative uncharacterized protein OS=Glycine max PE=2 SV=1)

HSP 1 Score: 219.935 bits (559), Expect = 2.952e-58
Identity = 100/126 (79.37%), Postives = 115/126 (91.27%), Query Frame = 3

Query:  144 EKLKTRVVKNNCNPEWNEELTLSIRDVKTPIHLTVYDKATFSVDDKMGNADVDLKPYVECVQMKLSSLPNGCAIKRIIPNRTNCLAEESSCVWKNGSIVQEMILRLKNVESGELVVEIEWVDVPGC 521
            +KLKTRVVKNNCNP+WNEELTLS++DVKTPIHLTVYDK TFSVDDKMG A++DLKPYV+C QM L  LPNGC++KRI P+RTN LAEESSC+W+NG IVQEM LRL+NVESGE++VEIEWVDV GC
Sbjct:   47 QKLKTRVVKNNCNPDWNEELTLSVKDVKTPIHLTVYDKDTFSVDDKMGGAEIDLKPYVQCKQMGLGKLPNGCSLKRIQPDRTNYLAEESSCIWQNGKIVQEMFLRLRNVESGEILVEIEWVDVVGC 172

HSP 2 Score: 30.4166 bits (67), Expect = 2.952e-58
Identity = 17/19 (89.47%), Postives = 17/19 (89.47%), Query Frame = 2

Query:   98 SMDNN-ILGLLKLRIKREV 151
            SMDNN ILGLLKLRIKR V
Sbjct:    8 SMDNNNILGLLKLRIKRGV 26

[Close]

BLAST of HO067833 vs. TrEMBL
Match: A5BXZ0_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_043576 PE=4 SV=1)

HSP 1 Score: 190.66 bits (483), Expect = 2.573e-47
Identity = 83/127 (65.35%), Postives = 107/127 (84.25%), Query Frame = 3

Query:  141 KEKLKTRVVKNNCNPEWNEELTLSIRDVKTPIHLTVYDKATFSVDDKMGNADVDLKPYVECVQMKLSSLPNGCAIKRIIPNRTNCLAEESSCVWKNGSIVQEMILRLKNVESGELVVEIEWVDVPGC 521
            ++KLKTRVVK+NCNPEWNEELTLSI D   PI+L VYD  TF++DDKMG+A++D+KPYVEC++M L +LP G  I R+ P+RTNCLA+ES CVW NG I Q+M+LRL+NVE GE+ V+IEW+++PGC
Sbjct:   36 EQKLKTRVVKDNCNPEWNEELTLSIADTDVPINLVVYDSDTFTLDDKMGDAEIDIKPYVECLKMGLENLPTGTVISRVQPSRTNCLADESCCVWDNGKIRQDMLLRLRNVECGEVEVQIEWINIPGC 162

HSP 2 Score: 23.0978 bits (48), Expect = 2.573e-47
Identity = 8/14 (57.14%), Postives = 13/14 (92.86%), Query Frame = 2

Query:  110 NILGLLKLRIKREV 151
            N+LGLL+LR++R +
Sbjct:    3 NLLGLLRLRVRRGI 16

[Close]

BLAST of HO067833 vs. TrEMBL
Match: D7T8Q5_VITVI (Whole genome shotgun sequence of line PN40024, scaffold_11.assembly12x (Fragment) OS=Vitis vinifera GN=VIT_00011712001 PE=4 SV=1)

HSP 1 Score: 190.66 bits (483), Expect = 7.010e-47
Identity = 83/127 (65.35%), Postives = 107/127 (84.25%), Query Frame = 3

Query:  141 KEKLKTRVVKNNCNPEWNEELTLSIRDVKTPIHLTVYDKATFSVDDKMGNADVDLKPYVECVQMKLSSLPNGCAIKRIIPNRTNCLAEESSCVWKNGSIVQEMILRLKNVESGELVVEIEWVDVPGC 521
            ++KLKTRVVK+NCNPEWNEELTLSI D   PI+L VYD  TF++DDKMG+A++D+KPYVEC++M L +LP G  I R+ P+RTNCLA+ES CVW NG I Q+M+LRL+NVE GE+ V+IEW+++PGC
Sbjct:    3 EQKLKTRVVKDNCNPEWNEELTLSIADTDVPINLVVYDSDTFTLDDKMGDAEIDIKPYVECLKMGLENLPTGTVISRVQPSRTNCLADESCCVWDNGKIRQDMLLRLRNVECGEVEVQIEWINIPGC 129

[Close]

BLAST of HO067833 vs. TrEMBL
Match: B9HW51_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_658758 PE=4 SV=1)

HSP 1 Score: 188.734 bits (478), Expect = 7.419e-47
Identity = 83/127 (65.35%), Postives = 107/127 (84.25%), Query Frame = 3

Query:  141 KEKLKTRVVKNNCNPEWNEELTLSIRDVKTPIHLTVYDKATFSVDDKMGNADVDLKPYVECVQMKLSSLPNGCAIKRIIPNRTNCLAEESSCVWKNGSIVQEMILRLKNVESGELVVEIEWVDVPGC 521
            K+KLKTRVVK NCNPEWNEELTLSI D+  PI+LTV+DK  F+VDDKMG A++D+K Y+  ++M L +LPNGC + RI P+R NCLA+ES  VW NG I+Q+MILRL+NVESGE++++IEW++VPGC
Sbjct:   36 KQKLKTRVVKKNCNPEWNEELTLSITDLNVPINLTVFDKDRFTVDDKMGEAEIDIKAYIASLKMGLQNLPNGCVVSRIKPSRNNCLADESCVVWDNGKILQDMILRLRNVESGEVMIQIEWMNVPGC 162

HSP 2 Score: 23.483 bits (49), Expect = 7.419e-47
Identity = 8/13 (61.54%), Postives = 13/13 (100.00%), Query Frame = 2

Query:  107 NNILGLLKLRIKR 145
            +N+LGLL++R+KR
Sbjct:    2 DNLLGLLRIRVKR 14

[Close]

BLAST of HO067833 vs. TrEMBL
Match: B9SP79_RICCO (ARF GTPase activator, putative OS=Ricinus communis GN=RCOM_0630050 PE=4 SV=1)

HSP 1 Score: 180.259 bits (456), Expect = 1.138e-44
Identity = 79/126 (62.70%), Postives = 101/126 (80.16%), Query Frame = 3

Query:  144 EKLKTRVVKNNCNPEWNEELTLSIRDVKTPIHLTVYDKATFSVDDKMGNADVDLKPYVECVQMKLSSLPNGCAIKRIIPNRTNCLAEESSCVWKNGSIVQEMILRLKNVESGELVVEIEWVDVPGC 521
            + LKTRVVK NCNPEWNEELTLSI D   PI L V+DK TF++DDKMG+A +D+KPY+  ++M L +LPNGC + R+ P++ NCLA+ES  VW NG I Q+M LRL+NVESGE+ V++EW+DVPGC
Sbjct:   37 QTLKTRVVKKNCNPEWNEELTLSIEDPIVPIKLAVFDKDTFTLDDKMGDAHIDIKPYIASLKMGLQTLPNGCVVSRVQPSKDNCLADESCIVWNNGKITQDMHLRLRNVESGEVEVQVEWIDVPGC 162

HSP 2 Score: 24.6386 bits (52), Expect = 1.138e-44
Identity = 10/14 (71.43%), Postives = 13/14 (92.86%), Query Frame = 2

Query:  110 NILGLLKLRIKREV 151
            NILGLL++R+KR V
Sbjct:    3 NILGLLRIRVKRGV 16

[Close]

BLAST of HO067833 vs. TrEMBL
Match: Q9S7J9_ARATH (At1g70790 OS=Arabidopsis thaliana GN=F15H11.4 PE=2 SV=1)

HSP 1 Score: 182.956 bits (463), Expect = 1.462e-44
Identity = 86/128 (67.19%), Postives = 102/128 (79.69%), Query Frame = 3

Query:  144 EKLKTRVVKNNCNPEWNEELTLSIRDVKTPIHLTVYDKATFSVDDKMGNADVDLKPYVECVQMKLS--SLPNGCAIKRIIPNRTNCLAEESSCVWKNGSIVQEMILRLKNVESGELVVEIEWVDVPGC 521
            +KLKTRV+ NNCNP WNE+LTLSI+DV  PI LTV+DK  FS DDKMG+A++D +P++E  QM+L    LPNGCAIKRI P RTNCLAEESS  W NG I+QEMILRLKNVE GE+ + +EW D PGC
Sbjct:   38 QKLKTRVINNNCNPVWNEQLTLSIKDVNDPIRLTVFDKDRFSGDDKMGDAEIDFRPFLEAHQMELDFQKLPNGCAIKRIRPGRTNCLAEESSITWSNGKIMQEMILRLKNVECGEVELMLEWTDGPGC 165

[Close]

BLAST of HO067833 vs. TrEMBL
Match: D7KYE5_ARALY (C2 domain-containing protein OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_476210 PE=4 SV=1)

HSP 1 Score: 182.57 bits (462), Expect = 1.909e-44
Identity = 86/128 (67.19%), Postives = 101/128 (78.91%), Query Frame = 3

Query:  144 EKLKTRVVKNNCNPEWNEELTLSIRDVKTPIHLTVYDKATFSVDDKMGNADVDLKPYVECVQMKLS--SLPNGCAIKRIIPNRTNCLAEESSCVWKNGSIVQEMILRLKNVESGELVVEIEWVDVPGC 521
            +KLKTRV+ NNCNP WNE+LTLSI+DV  PI LTV+DK  FS DDKMG+A++D +P++E  QM+L    LPNGCAIKRI P RTNCLAEESS  W NG I QEMILRLKNVE GE+ + +EW D PGC
Sbjct:   38 QKLKTRVINNNCNPVWNEQLTLSIKDVNDPIRLTVFDKDRFSGDDKMGDAEIDFRPFLEAHQMELDFQKLPNGCAIKRIRPGRTNCLAEESSITWSNGKIKQEMILRLKNVECGEVEIMLEWTDGPGC 165

[Close]

BLAST of HO067833 vs. TrEMBL
Match: B9HJD0_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_820673 PE=4 SV=1)

HSP 1 Score: 181.415 bits (459), Expect = 4.253e-44
Identity = 79/126 (62.70%), Postives = 103/126 (81.75%), Query Frame = 3

Query:  144 EKLKTRVVKNNCNPEWNEELTLSIRDVKTPIHLTVYDKATFSVDDKMGNADVDLKPYVECVQMKLSSLPNGCAIKRIIPNRTNCLAEESSCVWKNGSIVQEMILRLKNVESGELVVEIEWVDVPGC 521
            +KLKTRVVK NCNP WNEELTLSI D+  PI+LTV+DK TF+VDDKMG A +DL+PY+  ++M L +LP GC + R+ P++ NCLA+ES  VW +G + Q+MILRL+NVESGE+ ++IEW+DVPGC
Sbjct:   37 QKLKTRVVKKNCNPVWNEELTLSITDLNVPINLTVFDKDTFTVDDKMGEAGIDLQPYIASLKMGLQNLPKGCVVSRVQPSQNNCLADESCIVWDDGKLHQDMILRLRNVESGEVTIQIEWIDVPGC 162

[Close]

BLAST of HO067833 vs. TrEMBL
Match: B2Z3Q0_9SOLA (Pollen-specific C2 domain containing protein OS=Nicotiana sp. variant 'Rastroensis' PE=2 SV=1)

HSP 1 Score: 172.555 bits (436), Expect = 2.266e-42
Identity = 75/126 (59.52%), Postives = 103/126 (81.75%), Query Frame = 3

Query:  141 KEKLKTRVVKNNCNPEWNEELTLSIRDVKTPIHLTVYDKATFSVDDKMGNADVDLKPYVECVQMKLSSLPNGCAIKRIIPNRTNCLAEESSCVWKNGSIVQEMILRLKNVESGELVVEIEWVDVPG 518
            K+KLKTRV+K + NPEWNEELTLS+ D   P+ LTVYD  TFS+DDKMG+A+ D+KP+VE ++M L  LP+G  I R++P RTNCLAEES  VW++G +VQ+MILRL+NVE GE+ ++++W+++PG
Sbjct:   59 KQKLKTRVIKKDINPEWNEELTLSVSDPSLPVKLTVYDHDTFSMDDKMGDAEFDIKPFVEALKMNLDGLPSGTVITRVLPCRTNCLAEESRVVWQDGKVVQDMILRLRNVECGEVELQLQWIELPG 184

HSP 2 Score: 24.6386 bits (52), Expect = 2.266e-42
Identity = 10/18 (55.56%), Postives = 16/18 (88.89%), Query Frame = 2

Query:   98 SMDNNILGLLKLRIKREV 151
            S+ +N+LGLL+++IKR V
Sbjct:   21 SLMDNLLGLLRIKIKRGV 38

[Close]

BLAST of HO067833 vs. TAIR peptide
Match: AT1G70790.1 (| Symbols: | Calcium-dependent lipid-binding (CaLB domain) family protein | chr1:26700724-26702127 FORWARD LENGTH=185)

HSP 1 Score: 182.956 bits (463), Expect = 1.091e-46
Identity = 86/128 (67.19%), Postives = 102/128 (79.69%), Query Frame = 3

Query:  144 EKLKTRVVKNNCNPEWNEELTLSIRDVKTPIHLTVYDKATFSVDDKMGNADVDLKPYVECVQMKLS--SLPNGCAIKRIIPNRTNCLAEESSCVWKNGSIVQEMILRLKNVESGELVVEIEWVDVPGC 521
            +KLKTRV+ NNCNP WNE+LTLSI+DV  PI LTV+DK  FS DDKMG+A++D +P++E  QM+L    LPNGCAIKRI P RTNCLAEESS  W NG I+QEMILRLKNVE GE+ + +EW D PGC
Sbjct:   38 QKLKTRVINNNCNPVWNEQLTLSIKDVNDPIRLTVFDKDRFSGDDKMGDAEIDFRPFLEAHQMELDFQKLPNGCAIKRIRPGRTNCLAEESSITWSNGKIMQEMILRLKNVECGEVELMLEWTDGPGC 165

[Close]

BLAST of HO067833 vs. TAIR peptide
Match: AT1G70790.2 (| Symbols: | Calcium-dependent lipid-binding (CaLB domain) family protein | chr1:26700724-26702127 FORWARD LENGTH=185)

HSP 1 Score: 182.956 bits (463), Expect = 1.091e-46
Identity = 86/128 (67.19%), Postives = 102/128 (79.69%), Query Frame = 3

Query:  144 EKLKTRVVKNNCNPEWNEELTLSIRDVKTPIHLTVYDKATFSVDDKMGNADVDLKPYVECVQMKLS--SLPNGCAIKRIIPNRTNCLAEESSCVWKNGSIVQEMILRLKNVESGELVVEIEWVDVPGC 521
            +KLKTRV+ NNCNP WNE+LTLSI+DV  PI LTV+DK  FS DDKMG+A++D +P++E  QM+L    LPNGCAIKRI P RTNCLAEESS  W NG I+QEMILRLKNVE GE+ + +EW D PGC
Sbjct:   38 QKLKTRVINNNCNPVWNEQLTLSIKDVNDPIRLTVFDKDRFSGDDKMGDAEIDFRPFLEAHQMELDFQKLPNGCAIKRIRPGRTNCLAEESSITWSNGKIMQEMILRLKNVECGEVELMLEWTDGPGC 165

[Close]

BLAST of HO067833 vs. TAIR peptide
Match: AT1G70810.1 (| Symbols: | Calcium-dependent lipid-binding (CaLB domain) family protein | chr1:26704207-26705091 FORWARD LENGTH=165)

HSP 1 Score: 167.933 bits (424), Expect = 3.633e-42
Identity = 76/125 (60.80%), Postives = 99/125 (79.20%), Query Frame = 3

Query:  144 EKLKTRVVKNNCNPEWNEELTLSIRDVKTPIHLTVYDKATFSVDDKMGNADVDLKPYVECVQMKLSSLPNGCAIKRIIPNRTNCLAEESSCVWKNGSIVQEMILRLKNVESGELVVEIEWVDVPG 518
            +KLKTRVV+NNCNPEWNEELTL++R    P++L VYDK TF+  DKMG+A +D+KP++E  +M L  LP+G  IKR++PNR NCLAE SS V  NG IVQ MIL L+NVE GE+ +++EW+D+PG
Sbjct:   37 QKLKTRVVENNCNPEWNEELTLALRHPDEPVNLIVYDKDTFTSHDKMGDAKIDIKPFLEVHKMGLQELPDGTEIKRVVPNRENCLAEASSIVSNNGKIVQNMILLLRNVECGEVEIQLEWIDIPG 161

[Close]

BLAST of HO067833 vs. TAIR peptide
Match: AT2G01540.1 (| Symbols: | Calcium-dependent lipid-binding (CaLB domain) family protein | chr2:242297-243233 REVERSE LENGTH=180)

HSP 1 Score: 160.999 bits (406), Expect = 4.441e-40
Identity = 74/131 (56.49%), Postives = 100/131 (76.34%), Query Frame = 3

Query:  135 VLKEKLKTRVVKNNCNPEWNEELTLSIRDVKTPIHLTVYDKATFSVDDKMGNADVDLKPYVECVQ--MKLSSLPNGCAIKRIIPNRTNCLAEESSCVWKNGSIVQEMILRLKNVESGELVVEIEWVDVPGC 521
            V  + LKTRVVK NCNP WNEE+T++I+D   PI LTV+D   F+ DDKMG+A++D++PY+E ++  M+L  LPNGCAIKR+ P+R NCL++ESS VW NG I Q++ILRL NVE GE+ + +EW +  GC
Sbjct:   35 VADQTLKTRVVKKNCNPVWNEEMTVAIKDPNVPIRLTVFDWDKFTGDDKMGDANIDIQPYLEALKMGMELLRLPNGCAIKRVQPSRHNCLSDESSIVWNNGKITQDLILRLNNVECGEIEIMLEWHEGAGC 165

[Close]

BLAST of HO067833 vs. TAIR peptide
Match: AT1G70800.1 (| Symbols: | Calcium-dependent lipid-binding (CaLB domain) family protein | chr1:26702757-26703650 FORWARD LENGTH=174)

HSP 1 Score: 154.451 bits (389), Expect = 4.157e-38
Identity = 70/125 (56.00%), Postives = 95/125 (76.00%), Query Frame = 3

Query:  144 EKLKTRVVKNNCNPEWNEELTLSIRDVKTPIHLTVYDKATFSVDDKMGNADVDLKPYVECVQMKLSSLPNGCAIKRIIPNRTNCLAEESSCVWKNGSIVQEMILRLKNVESGELVVEIEWVDVPG 518
            +KLK+  VKNNCNPEWNEELTL+I D   P+ L VYDK TF+ DDKMG+A +D+KP+++  ++ L  LP+G  +KRI+P R NCL+E+S  V  NG IVQ+MIL LKNVE G++ +++EW+  PG
Sbjct:   46 QKLKSFTVKNNCNPEWNEELTLAIEDPNEPVKLMVYDKDTFTADDKMGDAQIDMKPFLDVHKLGLKELPHGKELKRIVPTRDNCLSEDSIIVSDNGKIVQDMILLLKNVECGKVEIQLEWLKNPG 170

[Close]

BLAST of HO067833 vs. TAIR peptide
Match: AT3G17980.1 (| Symbols: | Calcium-dependent lipid-binding (CaLB domain) family protein | chr3:6152417-6153115 FORWARD LENGTH=177)

HSP 1 Score: 150.984 bits (380), Expect = 7.775e-38
Identity = 64/126 (50.79%), Postives = 95/126 (75.40%), Query Frame = 3

Query:  141 KEKLKTRVVKNNCNPEWNEELTLSIRDVKTPIHLTVYDKATFSVDDKMGNADVDLKPYVECVQMKLSSLPNGCAIKRIIPNRTNCLAEESSCVWKNGSIVQEMILRLKNVESGELVVEIEWVDVPG 518
            K+KLKTRV+  + NPEWNE+LTLS+ D    + LTVYD   FS DDKMG+A+ ++KPY+E ++M+L  LP+G  +  + P+R NCLAEES   W +G +VQ+++LRL++VE GE+  +++W+D+PG
Sbjct:   48 KQKLKTRVINKDVNPEWNEDLTLSVTDSNLTVLLTVYDHDMFSKDDKMGDAEFEIKPYIEALRMQLDGLPSGTIVTTVKPSRRNCLAEESRVTWVDGKLVQDLVLRLRHVECGEVEAQLQWIDLPG 173

HSP 2 Score: 23.8682 bits (50), Expect = 7.775e-38
Identity = 10/18 (55.56%), Postives = 16/18 (88.89%), Query Frame = 2

Query:   98 SMDNNILGLLKLRIKREV 151
            S+ +++LGLL++RIKR V
Sbjct:   11 SLMDDLLGLLRIRIKRGV 28

[Close]

BLAST of HO067833 vs. TAIR peptide
Match: AT1G73580.1 (| Symbols: | Calcium-dependent lipid-binding (CaLB domain) family protein | chr1:27654841-27655518 FORWARD LENGTH=168)

HSP 1 Score: 150.599 bits (379), Expect = 2.241e-37
Identity = 68/126 (53.97%), Postives = 94/126 (74.60%), Query Frame = 3

Query:  141 KEKLKTRVVKNNCNPEWNEELTLSIRDVKTPIHLTVYDKATFSVDDKMGNADVDLKPYVECVQMKLSSLPNGCAIKRIIPNRTNCLAEESSCVWKNGSIVQEMILRLKNVESGELVVEIEWVDVPG 518
            ++KLKT+VVK N NP+W E+L+ ++ D   P+ L VYD   FS DDKMG+A++DLKPY+E ++M+LS LP+G  I  I P+R NCLAEES   W N  IVQ + LRL+NVE GE+ +E++W+D+PG
Sbjct:   39 RQKLKTKVVKQNVNPQWQEDLSFTVTDPNLPLTLIVYDHDFFSKDDKMGDAEIDLKPYIEALRMELSGLPDGTIISTIGPSRGNCLAEESYIRWINDRIVQHICLRLRNVERGEVEIELQWIDLPG 164

HSP 2 Score: 22.7126 bits (47), Expect = 2.241e-37
Identity = 8/18 (44.44%), Postives = 16/18 (88.89%), Query Frame = 2

Query:   98 SMDNNILGLLKLRIKREV 151
            S+ +N+LG+L++R++R V
Sbjct:    2 SLMDNLLGILRVRVQRGV 19

[Close]

BLAST of HO067833 vs. TAIR peptide
Match: AT1G48590.2 (| Symbols: | Calcium-dependent lipid-binding (CaLB domain) family protein | chr1:17962886-17964274 FORWARD LENGTH=200)

HSP 1 Score: 149.058 bits (375), Expect = 2.909e-37
Identity = 64/126 (50.79%), Postives = 94/126 (74.60%), Query Frame = 3

Query:  141 KEKLKTRVVKNNCNPEWNEELTLSIRDVKTPIHLTVYDKATFSVDDKMGNADVDLKPYVECVQMKLSSLPNGCAIKRIIPNRTNCLAEESSCVWKNGSIVQEMILRLKNVESGELVVEIEWVDVPG 518
            K+KLKTRV+  N NPEWNE+LTLS+ D    + LTVYD  TF+ DDKMG+A+  +KP+V  ++M L  LP+G  +  + P+R NCLAEES  +W +G +VQ+++LRL++VE GE+  +++W+D+PG
Sbjct:   72 KQKLKTRVIYKNVNPEWNEDLTLSVSDPNLTVLLTVYDYDTFTKDDKMGDAEFGIKPFVNALKMHLHDLPSGTIVTTVQPSRDNCLAEESRVIWSDGKLVQDIVLRLRHVECGEVEAQLQWIDLPG 197

HSP 2 Score: 23.8682 bits (50), Expect = 2.909e-37
Identity = 10/18 (55.56%), Postives = 16/18 (88.89%), Query Frame = 2

Query:   98 SMDNNILGLLKLRIKREV 151
            S+ +++LGLL++RIKR V
Sbjct:   35 SLMDSLLGLLRIRIKRGV 52

[Close]

BLAST of HO067833 vs. TAIR peptide
Match: AT1G48590.1 (| Symbols: | Calcium-dependent lipid-binding (CaLB domain) family protein | chr1:17962979-17964274 FORWARD LENGTH=169)

HSP 1 Score: 149.058 bits (375), Expect = 2.919e-37
Identity = 64/126 (50.79%), Postives = 94/126 (74.60%), Query Frame = 3

Query:  141 KEKLKTRVVKNNCNPEWNEELTLSIRDVKTPIHLTVYDKATFSVDDKMGNADVDLKPYVECVQMKLSSLPNGCAIKRIIPNRTNCLAEESSCVWKNGSIVQEMILRLKNVESGELVVEIEWVDVPG 518
            K+KLKTRV+  N NPEWNE+LTLS+ D    + LTVYD  TF+ DDKMG+A+  +KP+V  ++M L  LP+G  +  + P+R NCLAEES  +W +G +VQ+++LRL++VE GE+  +++W+D+PG
Sbjct:   41 KQKLKTRVIYKNVNPEWNEDLTLSVSDPNLTVLLTVYDYDTFTKDDKMGDAEFGIKPFVNALKMHLHDLPSGTIVTTVQPSRDNCLAEESRVIWSDGKLVQDIVLRLRHVECGEVEAQLQWIDLPG 166

HSP 2 Score: 23.8682 bits (50), Expect = 2.919e-37
Identity = 10/18 (55.56%), Postives = 16/18 (88.89%), Query Frame = 2

Query:   98 SMDNNILGLLKLRIKREV 151
            S+ +++LGLL++RIKR V
Sbjct:    4 SLMDSLLGLLRIRIKRGV 21

[Close]

BLAST of HO067833 vs. TAIR peptide
Match: AT1G23140.1 (| Symbols: | Calcium-dependent lipid-binding (CaLB domain) family protein | chr1:8202362-8203172 REVERSE LENGTH=165)

HSP 1 Score: 142.895 bits (359), Expect = 5.773e-35
Identity = 65/125 (52.00%), Postives = 92/125 (73.60%), Query Frame = 3

Query:  144 EKLKTRVVKNNCNPEWNEELTLSIRDVKTPIHLTVYDKATFSVDDKMGNADVDLKPYVECVQMKLSSLPNGCAIKRIIPNRTNCLAEESSCVWKNGSIVQEMILRLKNVESGELVVEIEWVDVPG 518
            +KLKTR V+N+CNPEW++ELTL I D    + L VYDK TF+  D MG+A++D+KP+ E     +  L NG  I+R+ P+  NCLAEES  ++ NG I+Q+MIL+L+NVESGE+ ++IEW++V G
Sbjct:   37 QKLKTRGVENSCNPEWDDELTLGINDPNQHVTLEVYDKDTFTSHDPMGDAEIDIKPFFEVQGTDIQELTNGTEIRRVKPSGDNCLAEESRIIFSNGKILQDMILQLRNVESGEVEIQIEWINVTG 161

HSP 2 Score: 22.3274 bits (46), Expect = 5.773e-35
Identity = 7/14 (50.00%), Postives = 13/14 (92.86%), Query Frame = 2

Query:  110 NILGLLKLRIKREV 151
            N++GLL++R+KR +
Sbjct:    3 NLVGLLRIRVKRGI 16

The following BLAST results are available for this feature:

BLAST of HO067833 vs. SwissProt
BLAST of HO067833 vs. TrEMBL
BLAST of HO067833 vs. TAIR peptide

BLAST of HO067833 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Swissprot)
Total hits: 3


Match Name	E-value	Identity	Description
AGD11_ARATH	1.680e-19	38.84	Probable ADP-ribosylation factor GTPase-activating...	[more]
AGD13_ARATH	1.857e-18	39.02	Probable ADP-ribosylation factor GTPase-activating...	[more]
AGD12_ARATH	1.019e-16	37.40	ADP-ribosylation factor GTPase-activating protein ...	[more]

BLAST of HO067833 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
C6TCZ1_SOYBN	1.595e-59	80.16	Putative uncharacterized protein OS=Glycine max PE...	[more]
C6SXX9_SOYBN	2.952e-58	79.37	Putative uncharacterized protein OS=Glycine max PE...	[more]
A5BXZ0_VITVI	2.573e-47	65.35	Putative uncharacterized protein OS=Vitis vinifera...	[more]
D7T8Q5_VITVI	7.010e-47	65.35	Whole genome shotgun sequence of line PN40024, sca...	[more]
B9HW51_POPTR	7.419e-47	65.35	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
B9SP79_RICCO	1.138e-44	62.70	ARF GTPase activator, putative OS=Ricinus communis...	[more]
Q9S7J9_ARATH	1.462e-44	67.19	At1g70790 OS=Arabidopsis thaliana GN=F15H11.4 PE=2...	[more]
D7KYE5_ARALY	1.909e-44	67.19	C2 domain-containing protein OS=Arabidopsis lyrata...	[more]
B9HJD0_POPTR	4.253e-44	62.70	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
B2Z3Q0_9SOLA	2.266e-42	59.52	Pollen-specific C2 domain containing protein OS=Ni...	[more]

BLAST of HO067833 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide)
Total hits: 10


Match Name	E-value	Identity	Description
AT1G70790.1	1.091e-46	67.19	\| Symbols: \| Calcium-dependent lipid-binding (CaL...	[more]
AT1G70790.2	1.091e-46	67.19	\| Symbols: \| Calcium-dependent lipid-binding (CaL...	[more]
AT1G70810.1	3.633e-42	60.80	\| Symbols: \| Calcium-dependent lipid-binding (CaL...	[more]
AT2G01540.1	4.441e-40	56.49	\| Symbols: \| Calcium-dependent lipid-binding (CaL...	[more]
AT1G70800.1	4.157e-38	56.00	\| Symbols: \| Calcium-dependent lipid-binding (CaL...	[more]
AT3G17980.1	7.775e-38	50.79	\| Symbols: \| Calcium-dependent lipid-binding (CaL...	[more]
AT1G73580.1	2.241e-37	53.97	\| Symbols: \| Calcium-dependent lipid-binding (CaL...	[more]
AT1G48590.2	2.909e-37	50.79	\| Symbols: \| Calcium-dependent lipid-binding (CaL...	[more]
AT1G48590.1	2.919e-37	50.79	\| Symbols: \| Calcium-dependent lipid-binding (CaL...	[more]
AT1G23140.1	5.773e-35	52.00	\| Symbols: \| Calcium-dependent lipid-binding (CaL...	[more]

InterPro

Analysis Name: InterProScan analysis for Cicer arietinum unigene v1
Date Performed: 2010-12-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000008	C2 calcium-dependent membrane targeting	PFAM	PF00168	C2	coord: 24..73 score: 2.6
IPR000008	C2 calcium-dependent membrane targeting	SMART	SM00239	no description	coord: 3..89 score: 8.2
IPR008973	C2 calcium/lipid-binding domain, CaLB	SUPERFAMILY	49562	C2 domain (Calcium/lipid-binding domain, CaLB)	coord: 20..147 score: 8.9
IPR018029	C2 membrane targeting protein	PROFILE	PS50004	C2	coord: 23..74 score: 9
None	No IPR available	GENE3D	2.60.40.150	no description	coord: 117..145 score: 2
None	No IPR available	PANTHER	PTHR23180	CENTAURIN/ARF	coord: 19..149 score: 8.5
None	No IPR available	PANTHER	PTHR23180:SF5	CENTAURIN/ARF-RELATED	coord: 19..149 score: 8.5
None	No IPR available	SEG	seg	seg	coord: 12..26 score

Properties


Property Name	Value
Singlet in analysis	Cicer arietinum unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>HO067833 ID=HO067833; Name=HO067833; organism=Cicer arietinum; type=EST; length=604bp
TGTTGCGAATTGTGTTTTTCTATTTTAACTCCGAAAGAAGTTCCATAAAC
TTCATATTCGTATCCGCGTGATAATTTATTCAATTTGTGTGTTGGATTCG
ATGGATAATAATATTCTTGGTCTTCTCAAACTTCGTATTAAAAGAGAAGT
TGAAGACTCGTGTAGTGAAAAACAACTGCAACCCTGAGTGGAATGAAGAA
TTAACCCTTTCTATAAGGGATGTTAAAACTCCAATACATTTGACAGTTTA
TGACAAAGCCACTTTCTCTGTGGATGACAAAATGGGTAATGCAGATGTAG
ACTTGAAACCATATGTTGAATGTGTACAAATGAAGTTGTCCTCTCTCCCA
AATGGTTGTGCAATTAAGAGGATTATACCAAATAGGACTAATTGCCTTGC
TGAAGAGAGTAGTTGTGTTTGGAAAAATGGAAGCATTGTCCAAGAAATGA
TTCTAAGATTGAAAAATGTTGAGAGTGGAGAACTTGTTGTGGAAATTGAG
TGGGTGGATGTTCCTGGTTGTAGAGGTCTATTAGGTATACAACTTTAAGG
TGGACGTTTCATTCAACAACAATCAACAAAGTCTTATCTCGTTTGGTGGA
ATTT

Annotated Terms

The following terms have been associated with this EST:

Vocabulary: **INTERPRO**
Term	Definition
IPR000008	C2_dom
IPR008973	C2 calcium/lipid-binding domain, CaLB
IPR018029	C2 membrane targeting protein

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