HO064034

EST Overview


Name	HO064034
Unique Name	HO064034
Type	EST
Organism	Cicer arietinum (chickpea)
Sequence length	501

Libraries


Library Name	Type
LIBEST_026411 Chickpea drought stressed cDNA SSH library AR2	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Cicer arietinum unigene v1	Cicer arietinum unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Cicer arietinum unigene v1	2010-12-27
Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide	2011-02-03
Homology Analysis: Cicer arietinum unigene v1 vs Swissprot	2010-12-28
Homology Analysis: Cicer arietinum unigene v1 vs Trembl	2010-12-28
InterProScan analysis for Cicer arietinum unigene v1	2010-12-29

Homology

[Close]

BLAST of HO064034 vs. SwissProt
Match: GUN17_ARATH (Endoglucanase 17 OS=Arabidopsis thaliana GN=At4g02290 PE=2 SV=1)

HSP 1 Score: 93.2041 bits (230), Expect = 8.408e-22
Identity = 42/50 (84.00%), Postives = 45/50 (90.00%), Query Frame = 2

Query:  197 TDYLLKATAHPNTIYVQVGDAKKDHACWERPEDMDTPRSVFKVDVNAPGS 346
            TDYLLKAT+ P+TIYVQVGDA KDH+CWERPEDMDT RSVFKVD N PGS
Sbjct:  146 TDYLLKATSQPDTIYVQVGDANKDHSCWERPEDMDTVRSVFKVDKNIPGS 195

HSP 2 Score: 30.4166 bits (67), Expect = 8.408e-22
Identity = 11/22 (50.00%), Postives = 19/22 (86.36%), Query Frame = 1

Query:  385 SLVFRRSDPTYSNILIKGLLSL 450
            ++VFR+SDP+YS +L+K  +S+
Sbjct:  209 AIVFRKSDPSYSKVLLKRAISV 230

[Close]

BLAST of HO064034 vs. SwissProt
Match: GUN1_ARATH (Endoglucanase 1 OS=Arabidopsis thaliana GN=CEL2 PE=2 SV=1)

HSP 1 Score: 94.7449 bits (234), Expect = 3.133e-21
Identity = 42/50 (84.00%), Postives = 45/50 (90.00%), Query Frame = 2

Query:  197 TDYLLKATAHPNTIYVQVGDAKKDHACWERPEDMDTPRSVFKVDVNAPGS 346
            TD+LLKAT+HP+TIYVQVGD   DHACWERPEDMDTPRSVFKVD N PGS
Sbjct:  137 TDFLLKATSHPDTIYVQVGDPNMDHACWERPEDMDTPRSVFKVDKNNPGS 186

HSP 2 Score: 26.9498 bits (58), Expect = 3.133e-21
Identity = 10/22 (45.45%), Postives = 18/22 (81.82%), Query Frame = 1

Query:  385 SLVFRRSDPTYSNILIKGLLSL 450
            S+VFR+ DP+YSN L++  +++
Sbjct:  200 SIVFRKCDPSYSNHLLQRAITV 221

[Close]

BLAST of HO064034 vs. SwissProt
Match: GUN17_ORYSJ (Endoglucanase 17 OS=Oryza sativa subsp. japonica GN=GLU13 PE=2 SV=1)

HSP 1 Score: 90.8929 bits (224), Expect = 2.100e-19
Identity = 39/50 (78.00%), Postives = 46/50 (92.00%), Query Frame = 2

Query:  197 TDYLLKATAHPNTIYVQVGDAKKDHACWERPEDMDTPRSVFKVDVNAPGS 346
            TDYL+KATA PNT+YVQVGDA +DH+CWERPEDMDTPR+V+KVD + PGS
Sbjct:  118 TDYLMKATATPNTVYVQVGDAFRDHSCWERPEDMDTPRTVYKVDPSHPGS 167

HSP 2 Score: 24.6386 bits (52), Expect = 2.100e-19
Identity = 10/16 (62.50%), Postives = 13/16 (81.25%), Query Frame = 1

Query:  385 SLVFRRSDPTYSNILI 432
            S+VFR +DP YSN L+
Sbjct:  181 SIVFRDADPDYSNRLL 196

[Close]

BLAST of HO064034 vs. SwissProt
Match: GUN3_ORYSJ (Endoglucanase 3 OS=Oryza sativa subsp. japonica GN=GLU8 PE=2 SV=1)

HSP 1 Score: 94.7449 bits (234), Expect = 2.469e-19
Identity = 46/93 (49.46%), Postives = 59/93 (63.44%), Query Frame = 2

Query:  197 TDYLLKATAHPNTIYVQVGDAKKDHACWERPEDMDTPRSVFKVDVNAPGSK-XXXXXXXXXXXXXXXXXXXXXHTPTF*SKGY*VFQFADKHK 472
            +DYLLKATAHP+T+YVQVGDA +DHACWERPEDMDTPR+V+KVD + PG+                       +     ++   VF+FADKH+
Sbjct:  129 SDYLLKATAHPDTVYVQVGDANRDHACWERPEDMDTPRTVYKVDPSTPGTDVAAETAAALAAASLVFRKSDPAYASRLVARAKRVFEFADKHR 221

[Close]

BLAST of HO064034 vs. SwissProt
Match: GUN6_ORYSJ (Endoglucanase 6 OS=Oryza sativa subsp. japonica GN=Os02g0733300 PE=2 SV=1)

HSP 1 Score: 91.2781 bits (225), Expect = 6.002e-19
Identity = 39/50 (78.00%), Postives = 46/50 (92.00%), Query Frame = 2

Query:  197 TDYLLKATAHPNTIYVQVGDAKKDHACWERPEDMDTPRSVFKVDVNAPGS 346
            TDYL+KATA PNT+YVQVGDA +DH+CWERPEDMDTPR+V+KVD + PGS
Sbjct:  130 TDYLMKATAKPNTVYVQVGDAFRDHSCWERPEDMDTPRTVYKVDPSHPGS 179

HSP 2 Score: 22.7126 bits (47), Expect = 6.002e-19
Identity = 9/16 (56.25%), Postives = 12/16 (75.00%), Query Frame = 1

Query:  385 SLVFRRSDPTYSNILI 432
            S+VFR +DP YS  L+
Sbjct:  193 SIVFRDADPAYSKRLL 208

[Close]

BLAST of HO064034 vs. SwissProt
Match: GUN8_ARATH (Endoglucanase 8 OS=Arabidopsis thaliana GN=CEL1 PE=2 SV=1)

HSP 1 Score: 78.1814 bits (191), Expect = 7.149e-16
Identity = 35/50 (70.00%), Postives = 40/50 (80.00%), Query Frame = 2

Query:  197 TDYLLKATAHPNTIYVQVGDAKKDHACWERPEDMDTPRSVFKVDVNAPGS 346
            TDYLLKATA P  ++VQVGDA  DH CWERPEDMDT R+V+K+D   PGS
Sbjct:  122 TDYLLKATAIPGVVFVQVGDAYSDHNCWERPEDMDTLRTVYKIDRAHPGS 171

HSP 2 Score: 25.409 bits (54), Expect = 7.149e-16
Identity = 9/16 (56.25%), Postives = 13/16 (81.25%), Query Frame = 1

Query:  385 SLVFRRSDPTYSNILI 432
            S+VFR+ DP YS +L+
Sbjct:  185 SIVFRKRDPAYSRLLL 200

[Close]

BLAST of HO064034 vs. SwissProt
Match: GUN4_ORYSJ (Endoglucanase 4 OS=Oryza sativa subsp. japonica GN=GLU14 PE=2 SV=1)

HSP 1 Score: 77.0258 bits (188), Expect = 7.544e-15
Identity = 36/50 (72.00%), Postives = 41/50 (82.00%), Query Frame = 2

Query:  200 DYLLKA-TAHPNTIYVQVGDAKKDHACWERPEDMDTPRSVFKVDVNAPGS 346
            DYLLKA TA P+T+YVQV D  +DH CWERPEDMDTPRSV+KV   +PGS
Sbjct:  135 DYLLKAATATPDTLYVQVADPYQDHRCWERPEDMDTPRSVYKVTPQSPGS 184

HSP 2 Score: 23.0978 bits (48), Expect = 7.544e-15
Identity = 10/16 (62.50%), Postives = 13/16 (81.25%), Query Frame = 1

Query:  385 SLVFRRSDPTYSNILI 432
            S+VFR SDP+YS  L+
Sbjct:  198 SIVFRVSDPSYSAKLL 213

[Close]

BLAST of HO064034 vs. SwissProt
Match: GUN19_ORYSJ (Endoglucanase 19 OS=Oryza sativa subsp. japonica GN=Os08g0114200 PE=2 SV=1)

HSP 1 Score: 74.7146 bits (182), Expect = 1.272e-14
Identity = 34/50 (68.00%), Postives = 39/50 (78.00%), Query Frame = 2

Query:  200 DYLLKA-TAHPNTIYVQVGDAKKDHACWERPEDMDTPRSVFKVDVNAPGS 346
            DYLLKA TA P  +YVQV D  +DH CWERPEDMDTPRSV++V  + PGS
Sbjct:  149 DYLLKAATATPGALYVQVADPNQDHRCWERPEDMDTPRSVYRVTADKPGS 198

HSP 2 Score: 24.6386 bits (52), Expect = 1.272e-14
Identity = 10/16 (62.50%), Postives = 13/16 (81.25%), Query Frame = 1

Query:  385 SLVFRRSDPTYSNILI 432
            S+VFRR+DP YS  L+
Sbjct:  212 SMVFRRADPAYSARLL 227

[Close]

BLAST of HO064034 vs. SwissProt
Match: GUN23_ORYSJ (Endoglucanase 23 OS=Oryza sativa subsp. japonica GN=GLU12 PE=2 SV=1)

HSP 1 Score: 76.6406 bits (187), Expect = 1.273e-14
Identity = 31/50 (62.00%), Postives = 37/50 (74.00%), Query Frame = 2

Query:  197 TDYLLKATAHPNTIYVQVGDAKKDHACWERPEDMDTPRSVFKVDVNAPGS 346
            TDYLLK  +HP  I++QVGD  KDH CWERPEDMDT R+V+ +    PGS
Sbjct:  137 TDYLLKTISHPGVIFIQVGDPTKDHGCWERPEDMDTARTVYNISAARPGS 186

HSP 2 Score: 22.7126 bits (47), Expect = 1.273e-14
Identity = 10/21 (47.62%), Postives = 13/21 (61.90%), Query Frame = 1

Query:  385 SLVFRRSDPTYSNILIKGLLS 447
            S+VFR  DP Y+  L+ G  S
Sbjct:  200 SMVFRDDDPAYAARLLAGARS 220

[Close]

BLAST of HO064034 vs. SwissProt
Match: GUN_PHAVU (Endoglucanase OS=Phaseolus vulgaris PE=2 SV=2)

HSP 1 Score: 78.1814 bits (191), Expect = 2.150e-14
Identity = 30/50 (60.00%), Postives = 40/50 (80.00%), Query Frame = 2

Query:  200 DYLLKATAHPNTIYVQVGDAKKDHACWERPEDMDTPRSVFKVDVNAPGSK 349
            D++L+A   P T+Y QVGD   DH CWERPEDMDTPR+V+K+D N+PG++
Sbjct:  137 DFMLRAHTSPTTLYTQVGDGNADHNCWERPEDMDTPRTVYKIDANSPGTE 186

HSP 2 Score: 20.4014 bits (41), Expect = 2.150e-14
Identity = 9/22 (40.91%), Postives = 14/22 (63.64%), Query Frame = 1

Query:  385 SLVFRRSDPTYSNILIKGLLSL 450
            S+VF++ D  YS+ L+    SL
Sbjct:  199 SIVFKKIDAKYSSTLLSHSKSL 220

[Close]

BLAST of HO064034 vs. TrEMBL
Match: Q2LAH6_SOYBN (Endo-1,4-beta-glucanase (Fragment) OS=Glycine max GN=Cel7 PE=2 SV=1)

HSP 1 Score: 104.375 bits (259), Expect = 6.354e-25
Identity = 46/51 (90.20%), Postives = 48/51 (94.12%), Query Frame = 2

Query:  197 TDYLLKATAHPNTIYVQVGDAKKDHACWERPEDMDTPRSVFKVDVNAPGSK 349
            TDYLLKATAHPNTIYVQVGDAKKDHACWERP DMDTPRSVFK+D N PGS+
Sbjct:   44 TDYLLKATAHPNTIYVQVGDAKKDHACWERPGDMDTPRSVFKIDANTPGSE 94

HSP 2 Score: 33.4982 bits (75), Expect = 6.354e-25
Identity = 20/40 (50.00%), Postives = 28/40 (70.00%), Query Frame = 1

Query:  385 SLVFRRSDPTYSNILIKGLLSL-SIC**TQGS-THALKPF 498
            SLVFRRSDPTYS IL++  + +       +GS ++ALKP+
Sbjct:  107 SLVFRRSDPTYSKILVRRAIRVFQFADKYRGSYSNALKPY 146

[Close]

BLAST of HO064034 vs. TrEMBL
Match: B9I9P6_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_824161 PE=4 SV=1)

HSP 1 Score: 102.064 bits (253), Expect = 5.578e-23
Identity = 45/51 (88.24%), Postives = 49/51 (96.08%), Query Frame = 2

Query:  197 TDYLLKATAHPNTIYVQVGDAKKDHACWERPEDMDTPRSVFKVDVNAPGSK 349
            TDYLLKATAHP+TIYVQVGDA KDHACWERPEDMDTPRSV+KVD N+PGS+
Sbjct:  140 TDYLLKATAHPDTIYVQVGDANKDHACWERPEDMDTPRSVYKVDKNSPGSE 190

HSP 2 Score: 29.261 bits (64), Expect = 5.578e-23
Identity = 11/17 (64.71%), Postives = 15/17 (88.24%), Query Frame = 1

Query:  385 SLVFRRSDPTYSNILIK 435
            SLVFRR DPTY+ +L++
Sbjct:  203 SLVFRRCDPTYAKLLVR 219

[Close]

BLAST of HO064034 vs. TrEMBL
Match: Q9SSU7_PEA (Endo-1,4-beta-glucanase OS=Pisum sativum GN=EGL2 PE=2 SV=1)

HSP 1 Score: 108.612 bits (270), Expect = 2.105e-22
Identity = 56/93 (60.22%), Postives = 60/93 (64.52%), Query Frame = 2

Query:  197 TDYLLKATAHPNTIYVQVGDAKKDHACWERPEDMDTPRSVFKVDVNAPGSK-XXXXXXXXXXXXXXXXXXXXXHTPTF*SKGY*VFQFADKHK 472
            TDYLLKATAHPN IYVQVGDAKKDHACWERPEDMDTPRSVFKVD NAPGS+                      +      +   VFQFADKH+
Sbjct:  136 TDYLLKATAHPNIIYVQVGDAKKDHACWERPEDMDTPRSVFKVDANAPGSEVAAETAAALAAASLVFRKSDPTYAKILVRRAIRVFQFADKHR 228

[Close]

BLAST of HO064034 vs. TrEMBL
Match: D7U2B8_VITVI (Whole genome shotgun sequence of line PN40024, scaffold_5.assembly12x (Fragment) OS=Vitis vinifera GN=VIT_00028042001 PE=4 SV=1)

HSP 1 Score: 97.4413 bits (241), Expect = 2.715e-22
Identity = 44/50 (88.00%), Postives = 45/50 (90.00%), Query Frame = 2

Query:  197 TDYLLKATAHPNTIYVQVGDAKKDHACWERPEDMDTPRSVFKVDVNAPGS 346
            TDYLLKAT HP+TIYVQVGDA KDHACWERPEDMDT RSVFKVD N PGS
Sbjct:   69 TDYLLKATVHPDTIYVQVGDANKDHACWERPEDMDTSRSVFKVDRNTPGS 118

HSP 2 Score: 31.5722 bits (70), Expect = 2.715e-22
Identity = 13/17 (76.47%), Postives = 16/17 (94.12%), Query Frame = 1

Query:  385 SLVFRRSDPTYSNILIK 435
            S+VFRRSDPTYS +LI+
Sbjct:  132 SVVFRRSDPTYSKLLIR 148

[Close]

BLAST of HO064034 vs. TrEMBL
Match: B9GSC6_POPTR (Glycosyl hydrolase family 9 OS=Populus trichocarpa GN=POPTRDRAFT_850247 PE=4 SV=1)

HSP 1 Score: 95.9005 bits (237), Expect = 3.761e-21
Identity = 43/50 (86.00%), Postives = 47/50 (94.00%), Query Frame = 2

Query:  197 TDYLLKATAHPNTIYVQVGDAKKDHACWERPEDMDTPRSVFKVDVNAPGS 346
            TDYLLKATAH +TIYVQVG+A KDHACWERPEDMDTPRSVFKVD ++PGS
Sbjct:  141 TDYLLKATAHTDTIYVQVGNANKDHACWERPEDMDTPRSVFKVDKHSPGS 190

HSP 2 Score: 29.261 bits (64), Expect = 3.761e-21
Identity = 11/17 (64.71%), Postives = 15/17 (88.24%), Query Frame = 1

Query:  385 SLVFRRSDPTYSNILIK 435
            SLVFRR DPTY+ +L++
Sbjct:  204 SLVFRRCDPTYAKLLVR 220

[Close]

BLAST of HO064034 vs. TrEMBL
Match: B9SAU5_RICCO (Endo-1,4-beta-glucanase, putative OS=Ricinus communis GN=RCOM_1179080 PE=4 SV=1)

HSP 1 Score: 102.834 bits (255), Expect = 1.155e-20
Identity = 54/96 (56.25%), Postives = 63/96 (65.62%), Query Frame = 2

Query:  197 TDYLLKATAHPNTIYVQVGDAKKDHACWERPEDMDTPRSVFKVDVNAPGSKXXXXXXXXXXXXXXXXXXXXXHTPTF*S----KGY*VFQFADKHK 472
            TDYLLKATAHP+TIYVQVGDA KDHACWERPEDMDTPRSVFK+D N+PGS+                     + PT+      +   VFQFADK++
Sbjct:  140 TDYLLKATAHPDTIYVQVGDANKDHACWERPEDMDTPRSVFKIDRNSPGSE---VAAETAAALAAASLVFKKNDPTYSKLLVRRAIRVFQFADKYR 232

[Close]

BLAST of HO064034 vs. TrEMBL
Match: Q42875_SOLLC (Endo-1,4-beta-glucanase OS=Solanum lycopersicum PE=2 SV=1)

HSP 1 Score: 94.7449 bits (234), Expect = 1.824e-20
Identity = 40/51 (78.43%), Postives = 47/51 (92.16%), Query Frame = 2

Query:  197 TDYLLKATAHPNTIYVQVGDAKKDHACWERPEDMDTPRSVFKVDVNAPGSK 349
            T+YLLKATAHP+TIYVQVGDA  DH+CWERPEDMDTPRSV+K+D N PG++
Sbjct:  140 TEYLLKATAHPDTIYVQVGDAGSDHSCWERPEDMDTPRSVYKIDKNTPGTE 190

HSP 2 Score: 28.1054 bits (61), Expect = 1.824e-20
Identity = 12/17 (70.59%), Postives = 15/17 (88.24%), Query Frame = 1

Query:  385 SLVFRRSDPTYSNILIK 435
            SLVFR+ +P+YS ILIK
Sbjct:  203 SLVFRKCNPSYSKILIK 219

[Close]

BLAST of HO064034 vs. TrEMBL
Match: Q9S948_SOLLC (CEL4=CELLULASE 4 (Fragment) OS=Solanum lycopersicum PE=4 SV=1)

HSP 1 Score: 94.7449 bits (234), Expect = 1.884e-20
Identity = 40/51 (78.43%), Postives = 47/51 (92.16%), Query Frame = 2

Query:  197 TDYLLKATAHPNTIYVQVGDAKKDHACWERPEDMDTPRSVFKVDVNAPGSK 349
            T+YLLKATAHP+TIYVQVGDA  DH+CWERPEDMDTPRSV+K+D N PG++
Sbjct:   48 TEYLLKATAHPDTIYVQVGDAGSDHSCWERPEDMDTPRSVYKIDKNTPGTE 98

HSP 2 Score: 28.1054 bits (61), Expect = 1.884e-20
Identity = 12/17 (70.59%), Postives = 15/17 (88.24%), Query Frame = 1

Query:  385 SLVFRRSDPTYSNILIK 435
            SLVFR+ +P+YS ILIK
Sbjct:  111 SLVFRKCNPSYSKILIK 127

[Close]

BLAST of HO064034 vs. TrEMBL
Match: D7M426_ARALY (Glycosyl hydrolase family 9 protein OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_490341 PE=4 SV=1)

HSP 1 Score: 93.2041 bits (230), Expect = 3.086e-20
Identity = 42/50 (84.00%), Postives = 45/50 (90.00%), Query Frame = 2

Query:  197 TDYLLKATAHPNTIYVQVGDAKKDHACWERPEDMDTPRSVFKVDVNAPGS 346
            TDYLLKAT+ P+TIYVQVGDA KDH+CWERPEDMDT RSVFKVD N PGS
Sbjct:  141 TDYLLKATSSPDTIYVQVGDANKDHSCWERPEDMDTVRSVFKVDKNTPGS 190

HSP 2 Score: 28.8758 bits (63), Expect = 3.086e-20
Identity = 10/17 (58.82%), Postives = 16/17 (94.12%), Query Frame = 1

Query:  385 SLVFRRSDPTYSNILIK 435
            ++VFR+SDP+YS +L+K
Sbjct:  204 AIVFRKSDPSYSKVLLK 220

[Close]

BLAST of HO064034 vs. TrEMBL
Match: D7KBC1_ARALY (ATCEL2 OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_887421 PE=4 SV=1)

HSP 1 Score: 94.7449 bits (234), Expect = 3.087e-20
Identity = 42/50 (84.00%), Postives = 45/50 (90.00%), Query Frame = 2

Query:  197 TDYLLKATAHPNTIYVQVGDAKKDHACWERPEDMDTPRSVFKVDVNAPGS 346
            TD+LLKAT+HP+TIYVQVGD   DHACWERPEDMDTPRSVFKVD N PGS
Sbjct:  137 TDFLLKATSHPDTIYVQVGDPNMDHACWERPEDMDTPRSVFKVDKNNPGS 186

HSP 2 Score: 27.335 bits (59), Expect = 3.087e-20
Identity = 10/22 (45.45%), Postives = 18/22 (81.82%), Query Frame = 1

Query:  385 SLVFRRSDPTYSNILIKGLLSL 450
            S+VF R DP+YSN+L++  +++
Sbjct:  200 SIVFGRCDPSYSNLLLQRAITV 221

[Close]

BLAST of HO064034 vs. TAIR peptide
Match: AT4G02290.1 (| Symbols: AtGH9B13, GH9B13 | glycosyl hydrolase 9B13 | chr4:1002654-1005125 REVERSE LENGTH=516)

HSP 1 Score: 93.2041 bits (230), Expect = 9.276e-23
Identity = 42/50 (84.00%), Postives = 45/50 (90.00%), Query Frame = 2

Query:  197 TDYLLKATAHPNTIYVQVGDAKKDHACWERPEDMDTPRSVFKVDVNAPGS 346
            TDYLLKAT+ P+TIYVQVGDA KDH+CWERPEDMDT RSVFKVD N PGS
Sbjct:  146 TDYLLKATSQPDTIYVQVGDANKDHSCWERPEDMDTVRSVFKVDKNIPGS 195

HSP 2 Score: 30.4166 bits (67), Expect = 9.276e-23
Identity = 11/22 (50.00%), Postives = 19/22 (86.36%), Query Frame = 1

Query:  385 SLVFRRSDPTYSNILIKGLLSL 450
            ++VFR+SDP+YS +L+K  +S+
Sbjct:  209 AIVFRKSDPSYSKVLLKRAISV 230

[Close]

BLAST of HO064034 vs. TAIR peptide
Match: AT1G02800.1 (| Symbols: ATCEL2, CEL2 | cellulase 2 | chr1:613386-616103 REVERSE LENGTH=501)

HSP 1 Score: 94.7449 bits (234), Expect = 3.456e-22
Identity = 42/50 (84.00%), Postives = 45/50 (90.00%), Query Frame = 2

Query:  197 TDYLLKATAHPNTIYVQVGDAKKDHACWERPEDMDTPRSVFKVDVNAPGS 346
            TD+LLKAT+HP+TIYVQVGD   DHACWERPEDMDTPRSVFKVD N PGS
Sbjct:  137 TDFLLKATSHPDTIYVQVGDPNMDHACWERPEDMDTPRSVFKVDKNNPGS 186

HSP 2 Score: 26.9498 bits (58), Expect = 3.456e-22
Identity = 10/22 (45.45%), Postives = 18/22 (81.82%), Query Frame = 1

Query:  385 SLVFRRSDPTYSNILIKGLLSL 450
            S+VFR+ DP+YSN L++  +++
Sbjct:  200 SIVFRKCDPSYSNHLLQRAITV 221

[Close]

BLAST of HO064034 vs. TAIR peptide
Match: AT1G70710.1 (| Symbols: ATGH9B1, CEL1, GH9B1 | glycosyl hydrolase 9B1 | chr1:26659356-26662962 REVERSE LENGTH=492)

HSP 1 Score: 78.1814 bits (191), Expect = 7.879e-17
Identity = 35/50 (70.00%), Postives = 40/50 (80.00%), Query Frame = 2

Query:  197 TDYLLKATAHPNTIYVQVGDAKKDHACWERPEDMDTPRSVFKVDVNAPGS 346
            TDYLLKATA P  ++VQVGDA  DH CWERPEDMDT R+V+K+D   PGS
Sbjct:  122 TDYLLKATAIPGVVFVQVGDAYSDHNCWERPEDMDTLRTVYKIDRAHPGS 171

HSP 2 Score: 25.409 bits (54), Expect = 7.879e-17
Identity = 9/16 (56.25%), Postives = 13/16 (81.25%), Query Frame = 1

Query:  385 SLVFRRSDPTYSNILI 432
            S+VFR+ DP YS +L+
Sbjct:  185 SIVFRKRDPAYSRLLL 200

[Close]

BLAST of HO064034 vs. TAIR peptide
Match: AT1G23210.1 (| Symbols: AtGH9B6, GH9B6 | glycosyl hydrolase 9B6 | chr1:8240174-8242129 FORWARD LENGTH=489)

HSP 1 Score: 74.7146 bits (182), Expect = 3.997e-15
Identity = 32/51 (62.75%), Postives = 41/51 (80.39%), Query Frame = 2

Query:  197 TDYLLKATAHPNTIYVQVGDAKKDHACWERPEDMDTPRSVFKVDVNAPGSK 349
            TDYL+KAT  P+ ++VQVGDA  DH CWERPEDMDT R+V+K+D +  GS+
Sbjct:  122 TDYLMKATQIPDVVFVQVGDAYSDHNCWERPEDMDTLRTVYKIDKDHSGSE 172

HSP 2 Score: 23.0978 bits (48), Expect = 3.997e-15
Identity = 8/16 (50.00%), Postives = 12/16 (75.00%), Query Frame = 1

Query:  385 SLVFRRSDPTYSNILI 432
            S+VF + DP YS +L+
Sbjct:  185 SIVFEKRDPVYSKMLL 200

[Close]

BLAST of HO064034 vs. TAIR peptide
Match: AT1G75680.1 (| Symbols: AtGH9B7, GH9B7 | glycosyl hydrolase 9B7 | chr1:28417215-28419231 REVERSE LENGTH=525)

HSP 1 Score: 69.707 bits (169), Expect = 6.732e-15
Identity = 29/51 (56.86%), Postives = 37/51 (72.55%), Query Frame = 2

Query:  197 TDYLLKATAHPNTIYVQVGDAKKDHACWERPEDMDTPRSVFKVDVNAPGSK 349
            TDYL+KA    N +Y+QVGD K DH CWERPEDM   R + K+DV+ PG++
Sbjct:  152 TDYLIKAHPSDNVLYIQVGDPKVDHPCWERPEDMKEKRPLTKIDVDTPGTE 202

HSP 2 Score: 27.335 bits (59), Expect = 6.732e-15
Identity = 12/17 (70.59%), Postives = 14/17 (82.35%), Query Frame = 1

Query:  385 SLVFRRSDPTYSNILIK 435
            SLVF+ SDPTYS  L+K
Sbjct:  215 SLVFKDSDPTYSATLLK 231

[Close]

BLAST of HO064034 vs. TAIR peptide
Match: AT4G11050.1 (| Symbols: AtGH9C3, GH9C3 | glycosyl hydrolase 9C3 | chr4:6747498-6751311 REVERSE LENGTH=626)

HSP 1 Score: 69.3218 bits (168), Expect = 1.131e-14
Identity = 28/50 (56.00%), Postives = 35/50 (70.00%), Query Frame = 2

Query:  197 TDYLLKATAHPNTIYVQVGDAKKDHACWERPEDMDTPRSVFKVDVNAPGS 346
            TDY +KA   PN +Y +VGD K DH CW+RPE+M T R  +K+D N PGS
Sbjct:  122 TDYFIKAHPEPNVLYGEVGDGKSDHYCWQRPEEMTTDRRAYKIDRNNPGS 171

HSP 2 Score: 26.9498 bits (58), Expect = 1.131e-14
Identity = 11/17 (64.71%), Postives = 15/17 (88.24%), Query Frame = 1

Query:  385 SLVFRRSDPTYSNILIK 435
            S+VFRRSDP+YS  L++
Sbjct:  185 SIVFRRSDPSYSAELLR 201

[Close]

BLAST of HO064034 vs. TAIR peptide
Match: AT1G22880.1 (| Symbols: ATGH9B4, ATCEL5, CEL5 | cellulase 5 | chr1:8095770-8097539 FORWARD LENGTH=484)

HSP 1 Score: 69.707 bits (169), Expect = 4.204e-14
Identity = 33/51 (64.71%), Postives = 37/51 (72.55%), Query Frame = 2

Query:  197 TDYLLK-ATAHPNTIYVQVGDAKKDHACWERPEDMDTPRSVFKVDVNAPGS 346
            TDYLLK A A P  +YV VGD   DH CWERPEDMDTPR+V+ V  + PGS
Sbjct:  118 TDYLLKCARATPGKLYVGVGDPNGDHKCWERPEDMDTPRTVYSVSPSNPGS 168

HSP 2 Score: 24.6386 bits (52), Expect = 4.204e-14
Identity = 9/16 (56.25%), Postives = 13/16 (81.25%), Query Frame = 1

Query:  385 SLVFRRSDPTYSNILI 432
            S+VFR+ DP YS +L+
Sbjct:  182 SMVFRKVDPKYSRLLL 197

[Close]

BLAST of HO064034 vs. TAIR peptide
Match: AT1G22880.2 (| Symbols: ATGH9B4, ATCEL5, CEL5 | cellulase 5 | chr1:8096103-8097539 FORWARD LENGTH=400)

HSP 1 Score: 69.707 bits (169), Expect = 4.226e-14
Identity = 33/51 (64.71%), Postives = 37/51 (72.55%), Query Frame = 2

Query:  197 TDYLLK-ATAHPNTIYVQVGDAKKDHACWERPEDMDTPRSVFKVDVNAPGS 346
            TDYLLK A A P  +YV VGD   DH CWERPEDMDTPR+V+ V  + PGS
Sbjct:   34 TDYLLKCARATPGKLYVGVGDPNGDHKCWERPEDMDTPRTVYSVSPSNPGS 84

HSP 2 Score: 24.6386 bits (52), Expect = 4.226e-14
Identity = 9/16 (56.25%), Postives = 13/16 (81.25%), Query Frame = 1

Query:  385 SLVFRRSDPTYSNILI 432
            S+VFR+ DP YS +L+
Sbjct:   98 SMVFRKVDPKYSRLLL 113

[Close]

BLAST of HO064034 vs. TAIR peptide
Match: AT4G23560.1 (| Symbols: AtGH9B15, GH9B15 | glycosyl hydrolase 9B15 | chr4:12293633-12295788 REVERSE LENGTH=479)

HSP 1 Score: 71.633 bits (174), Expect = 9.207e-14
Identity = 27/51 (52.94%), Postives = 39/51 (76.47%), Query Frame = 2

Query:  197 TDYLLKATAHPNTIYVQVGDAKKDHACWERPEDMDTPRSVFKVDVNAPGSK 349
            TD++L+A   PN +Y QVGD   DH+CWERPEDMDT R+++ +  ++PGS+
Sbjct:  119 TDFILRAHTSPNMLYTQVGDGNSDHSCWERPEDMDTSRTLYSISSSSPGSE 169

HSP 2 Score: 21.557 bits (44), Expect = 9.207e-14
Identity = 9/16 (56.25%), Postives = 12/16 (75.00%), Query Frame = 1

Query:  385 SLVFRRSDPTYSNILI 432
            SLVF+  D TYS+ L+
Sbjct:  182 SLVFKSVDSTYSSTLL 197

[Close]

BLAST of HO064034 vs. TAIR peptide
Match: AT4G09740.1 (| Symbols: AtGH9B14, GH9B14 | glycosyl hydrolase 9B14 | chr4:6142706-6145003 REVERSE LENGTH=478)

HSP 1 Score: 69.707 bits (169), Expect = 2.015e-13
Identity = 26/51 (50.98%), Postives = 39/51 (76.47%), Query Frame = 2

Query:  197 TDYLLKATAHPNTIYVQVGDAKKDHACWERPEDMDTPRSVFKVDVNAPGSK 349
            T+++L+A    N +Y QVGD   DH+CWERPEDMDTPR+++ +  ++PGS+
Sbjct:  119 TNFILRAHTSTNMLYTQVGDGNSDHSCWERPEDMDTPRTLYSISSSSPGSE 169

HSP 2 Score: 22.3274 bits (46), Expect = 2.015e-13
Identity = 11/22 (50.00%), Postives = 14/22 (63.64%), Query Frame = 1

Query:  385 SLVFRRSDPTYSNILIKGLLSL 450
            SLVF+  D TYS+ L+    SL
Sbjct:  182 SLVFKLVDSTYSSKLLNNAKSL 203

The following BLAST results are available for this feature:

BLAST of HO064034 vs. SwissProt
BLAST of HO064034 vs. TrEMBL
BLAST of HO064034 vs. TAIR peptide

BLAST of HO064034 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Swissprot)
Total hits: 10


Match Name	E-value	Identity	Description
GUN17_ARATH	8.408e-22	84.00	Endoglucanase 17 OS=Arabidopsis thaliana GN=At4g02...	[more]
GUN1_ARATH	3.133e-21	84.00	Endoglucanase 1 OS=Arabidopsis thaliana GN=CEL2 PE...	[more]
GUN17_ORYSJ	2.100e-19	78.00	Endoglucanase 17 OS=Oryza sativa subsp. japonica G...	[more]
GUN3_ORYSJ	2.469e-19	49.46	Endoglucanase 3 OS=Oryza sativa subsp. japonica GN...	[more]
GUN6_ORYSJ	6.002e-19	78.00	Endoglucanase 6 OS=Oryza sativa subsp. japonica GN...	[more]
GUN8_ARATH	7.149e-16	70.00	Endoglucanase 8 OS=Arabidopsis thaliana GN=CEL1 PE...	[more]
GUN4_ORYSJ	7.544e-15	72.00	Endoglucanase 4 OS=Oryza sativa subsp. japonica GN...	[more]
GUN19_ORYSJ	1.272e-14	68.00	Endoglucanase 19 OS=Oryza sativa subsp. japonica G...	[more]
GUN23_ORYSJ	1.273e-14	62.00	Endoglucanase 23 OS=Oryza sativa subsp. japonica G...	[more]
GUN_PHAVU	2.150e-14	60.00	Endoglucanase OS=Phaseolus vulgaris PE=2 SV=2	[more]

BLAST of HO064034 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
Q2LAH6_SOYBN	6.354e-25	90.20	Endo-1,4-beta-glucanase (Fragment) OS=Glycine max ...	[more]
B9I9P6_POPTR	5.578e-23	88.24	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
Q9SSU7_PEA	2.105e-22	60.22	Endo-1,4-beta-glucanase OS=Pisum sativum GN=EGL2 P...	[more]
D7U2B8_VITVI	2.715e-22	88.00	Whole genome shotgun sequence of line PN40024, sca...	[more]
B9GSC6_POPTR	3.761e-21	86.00	Glycosyl hydrolase family 9 OS=Populus trichocarpa...	[more]
B9SAU5_RICCO	1.155e-20	56.25	Endo-1,4-beta-glucanase, putative OS=Ricinus commu...	[more]
Q42875_SOLLC	1.824e-20	78.43	Endo-1,4-beta-glucanase OS=Solanum lycopersicum PE...	[more]
Q9S948_SOLLC	1.884e-20	78.43	CEL4=CELLULASE 4 (Fragment) OS=Solanum lycopersicu...	[more]
D7M426_ARALY	3.086e-20	84.00	Glycosyl hydrolase family 9 protein OS=Arabidopsis...	[more]
D7KBC1_ARALY	3.087e-20	84.00	ATCEL2 OS=Arabidopsis lyrata subsp. lyrata GN=ARAL...	[more]

BLAST of HO064034 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide)
Total hits: 10


Match Name	E-value	Identity	Description
AT4G02290.1	9.276e-23	84.00	\| Symbols: AtGH9B13, GH9B13 \| glycosyl hydrolase 9...	[more]
AT1G02800.1	3.456e-22	84.00	\| Symbols: ATCEL2, CEL2 \| cellulase 2 \| chr1:61338...	[more]
AT1G70710.1	7.879e-17	70.00	\| Symbols: ATGH9B1, CEL1, GH9B1 \| glycosyl hydrola...	[more]
AT1G23210.1	3.997e-15	62.75	\| Symbols: AtGH9B6, GH9B6 \| glycosyl hydrolase 9B6...	[more]
AT1G75680.1	6.732e-15	56.86	\| Symbols: AtGH9B7, GH9B7 \| glycosyl hydrolase 9B7...	[more]
AT4G11050.1	1.131e-14	56.00	\| Symbols: AtGH9C3, GH9C3 \| glycosyl hydrolase 9C3...	[more]
AT1G22880.1	4.204e-14	64.71	\| Symbols: ATGH9B4, ATCEL5, CEL5 \| cellulase 5 \| c...	[more]
AT1G22880.2	4.226e-14	64.71	\| Symbols: ATGH9B4, ATCEL5, CEL5 \| cellulase 5 \| c...	[more]
AT4G23560.1	9.207e-14	52.94	\| Symbols: AtGH9B15, GH9B15 \| glycosyl hydrolase 9...	[more]
AT4G09740.1	2.015e-13	50.98	\| Symbols: AtGH9B14, GH9B14 \| glycosyl hydrolase 9...	[more]

InterPro

Analysis Name: InterProScan analysis for Cicer arietinum unigene v1
Date Performed: 2010-12-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001701	Glycoside hydrolase, family 9	PANTHER	PTHR22298:SF3	ENDO-1,4-BETA-GLUCANASE	coord: 15..64 score: 4.5
IPR001701	Glycoside hydrolase, family 9	PFAM	PF00759	Glyco_hydro_9	coord: 15..66 score: 9
IPR008928	Six-hairpin glycosidase-like	SUPERFAMILY	48208	Six-hairpin glycosidases	coord: 15..83 score: 2.6
IPR012341	Six-hairpin glycosidase	GENE3D	1.50.10.10	no description	coord: 15..67 score: 3
None	No IPR available	PANTHER	PTHR22298	ENDO-1,4-BETA-GLUCANASE	coord: 15..64 score: 4.5
None	No IPR available	SEG	seg	seg	coord: 66..86 score
None	No IPR available	SEG	seg	seg	coord: 15..22 score
None	No IPR available	SEG	seg	seg	coord: 40..50 score

Properties


Property Name	Value
Singlet in analysis	Cicer arietinum unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>HO064034 ID=HO064034; Name=HO064034; organism=Cicer arietinum; type=EST; length=501bp
CGGGCAGGTACTTTGAATTATAAAAAACAAATCCAGCATGCCAGCATAAA
AGGCTAGCGGTGAGGCAAAAATTTATCTATCGAACATACACCACTTTCAA
TTCTTGGAACAATTAAAAAAACCTAACAATGTAAGCAAATCTAGGCCTCT
GTGACAGGATACTCAAACACAACATCTTTTCCAGACAACTTCCTGTACTG
ATTATCTTCTTAAAGCCACTGCCCATCCAAACACCATCTATGTTCAGGTA
GGAGATGCTAAGAAGGACCATGCTTGTTGGGAGAGACCAGAGGACATGGA
CACACCAAGAAGTGTTTTTAAGGTAGATGTAAATGCACCTGGTTCAAAGT
TGCTGCGGAAACTGCTGCAGCACTTGCAGCTGCTTCTTTGGTTTTTAGAA
GAAGTGACCCTACATACTCCAACATTTTAATCAAAGGGCTATTAAGTCTT
TCAATTTGCTGATAAACACAAGGATCTACGCATGCATTGAAGCCCTTTGG
G

Annotated Terms

The following terms have been associated with this EST:

Vocabulary: **Biological Process**
Term	Definition
GO:0005975	carbohydrate metabolic process

Vocabulary: **Molecular Function**
Term	Definition
GO:0003824	catalytic activity

Vocabulary: **INTERPRO**
Term	Definition
IPR001701	Glyco_hydro_9
IPR008928	6-hairpin_glycosidase_sf
IPR012341	6hp_glycosidase-like_sf

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