HO063997

EST Overview


Name	HO063997
Unique Name	HO063997
Type	EST
Organism	Cicer arietinum (chickpea)
Sequence length	469

Libraries


Library Name	Type
LIBEST_026410 Chickpea drought stressed cDNA SSH library AR1	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Cicer arietinum unigene v1	Cicer arietinum unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Cicer arietinum unigene v1	2010-12-27
Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide	2011-02-03
KEGG analysis for Cicer arietinum unigene v1	2011-01-05
Homology Analysis: Cicer arietinum unigene v1 vs Trembl	2010-12-28
InterProScan analysis for Cicer arietinum unigene v1	2010-12-29

Homology

[Close]

BLAST of HO063997 vs. TrEMBL
Match: B7FLW9_MEDTR (Putative uncharacterized protein OS=Medicago truncatula PE=2 SV=1)

HSP 1 Score: 185.652 bits (470), Expect = 1.341e-45
Identity = 91/100 (91.00%), Postives = 91/100 (91.00%), Query Frame = 1

Query:    1 FKIFKGEVLEGVVHKVLKHGVFMRIGPIENAYLSSSKMPGYVYVLGENPYFMNQKMPKIAXXXXXXXXXIGTKWMEAEREFQALVGLEGDYLGPISSPDM 300
            FKIFKGEVLEGVVHKVLKHGVFMRIGPIENAYLSSSKMPGYVYVLGENPYFMNQKMPKIA         IGTKWMEAEREFQALVGLEGDYLGPISSPDM
Sbjct:   81 FKIFKGEVLEGVVHKVLKHGVFMRIGPIENAYLSSSKMPGYVYVLGENPYFMNQKMPKIAKDVKARVVVIGTKWMEAEREFQALVGLEGDYLGPISSPDM 180

[Close]

BLAST of HO063997 vs. TrEMBL
Match: C6T5N3_SOYBN (Putative uncharacterized protein OS=Glycine max PE=2 SV=1)

HSP 1 Score: 150.599 bits (379), Expect = 4.782e-35
Identity = 74/100 (74.00%), Postives = 78/100 (78.00%), Query Frame = 1

Query:    1 FKIFKGEVLEGVVHKVLKHGVFMRIGPIENAYLSSSKMPGYVYVLGENPYFMNQKMPKIAXXXXXXXXXIGTKWMEAEREFQALVGLEGDYLGPISSPDM 300
            FK FKGE+LEGVVHKVLKHGVFMR GPIEN YLS+ KMP Y YV GEN  FMN KM KI          IGTKWMEAEREFQALV LEGDYLGPIS+PD+
Sbjct:   81 FKFFKGEILEGVVHKVLKHGVFMRCGPIENVYLSNLKMPDYRYVPGENACFMNDKMSKIGKDVTVRFSVIGTKWMEAEREFQALVSLEGDYLGPISTPDI 180

[Close]

BLAST of HO063997 vs. TrEMBL
Match: B9S9D8_RICCO (DNA-directed RNA polymerase II 19 kD polypeptide rpb7, putative OS=Ricinus communis GN=RCOM_0884250 PE=4 SV=1)

HSP 1 Score: 140.969 bits (354), Expect = 3.789e-32
Identity = 68/96 (70.83%), Postives = 74/96 (77.08%), Query Frame = 1

Query:    1 FKIFKGEVLEGVVHKVLKHGVFMRIGPIENAYLSSSKMPGYVYVLGENPYFMNQKMPKIAXXXXXXXXXIGTKWMEAEREFQALVGLEGDYLGPIS 288
            FKIF+GE+LEGVVHKVLKHGVF+R GPIEN YLS  KMP Y YV GENP F+N K  KI          IGTKW+EAEREFQALV LEGDYLGP+S
Sbjct:   81 FKIFRGEILEGVVHKVLKHGVFIRCGPIENIYLSCMKMPDYHYVPGENPVFLNDKTSKIEKDVVVRFIVIGTKWLEAEREFQALVSLEGDYLGPVS 176

[Close]

BLAST of HO063997 vs. TrEMBL
Match: D7MGZ2_ARALY (RNA polymerase Rpb7 N-terminal domain-containing protein OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_915395 PE=4 SV=1)

HSP 1 Score: 139.813 bits (351), Expect = 8.441e-32
Identity = 67/96 (69.79%), Postives = 73/96 (76.04%), Query Frame = 1

Query:    1 FKIFKGEVLEGVVHKVLKHGVFMRIGPIENAYLSSSKMPGYVYVLGENPYFMNQKMPKIAXXXXXXXXXIGTKWMEAEREFQALVGLEGDYLGPIS 288
            FKIFKGE++ GVVHKVLKHGVFMR GPIEN YLS +KMP Y YV GENP FMN+K  +I          IG KWMEAEREFQAL  LEGDYLGP+S
Sbjct:   81 FKIFKGEIIHGVVHKVLKHGVFMRCGPIENVYLSYTKMPDYKYVPGENPIFMNEKTSRIQVETTVRVVVIGIKWMEAEREFQALASLEGDYLGPLS 176

[Close]

BLAST of HO063997 vs. TrEMBL
Match: O23322_ARATH (RNA polymerase II fifth largest subunit like protein OS=Arabidopsis thaliana GN=dl3370c PE=2 SV=1)

HSP 1 Score: 137.887 bits (346), Expect = 3.208e-31
Identity = 65/96 (67.71%), Postives = 72/96 (75.00%), Query Frame = 1

Query:    1 FKIFKGEVLEGVVHKVLKHGVFMRIGPIENAYLSSSKMPGYVYVLGENPYFMNQKMPKIAXXXXXXXXXIGTKWMEAEREFQALVGLEGDYLGPIS 288
            FKIFKGE++ GVVHKVLKHGVFMR GPIEN YLS +KMP Y Y+ GENP FMN+K  +I          IG KWME EREFQAL  LEGDYLGP+S
Sbjct:   64 FKIFKGEIIHGVVHKVLKHGVFMRCGPIENVYLSYTKMPDYKYIPGENPIFMNEKTSRIQVETTVRVVVIGIKWMEVEREFQALASLEGDYLGPLS 159

[Close]

BLAST of HO063997 vs. TrEMBL
Match: A6QRA1_ARATH (At4g14660 OS=Arabidopsis thaliana GN=At4g14660 PE=2 SV=1)

HSP 1 Score: 137.887 bits (346), Expect = 3.208e-31
Identity = 65/96 (67.71%), Postives = 72/96 (75.00%), Query Frame = 1

Query:    1 FKIFKGEVLEGVVHKVLKHGVFMRIGPIENAYLSSSKMPGYVYVLGENPYFMNQKMPKIAXXXXXXXXXIGTKWMEAEREFQALVGLEGDYLGPIS 288
            FKIFKGE++ GVVHKVLKHGVFMR GPIEN YLS +KMP Y Y+ GENP FMN+K  +I          IG KWME EREFQAL  LEGDYLGP+S
Sbjct:   81 FKIFKGEIIHGVVHKVLKHGVFMRCGPIENVYLSYTKMPDYKYIPGENPIFMNEKTSRIQVETTVRVVVIGIKWMEVEREFQALASLEGDYLGPLS 176

[Close]

BLAST of HO063997 vs. TrEMBL
Match: D7T792_VITVI (Whole genome shotgun sequence of line PN40024, scaffold_20.assembly12x (Fragment) OS=Vitis vinifera GN=VIT_00018108001 PE=4 SV=1)

HSP 1 Score: 137.502 bits (345), Expect = 4.189e-31
Identity = 63/96 (65.62%), Postives = 75/96 (78.12%), Query Frame = 1

Query:    1 FKIFKGEVLEGVVHKVLKHGVFMRIGPIENAYLSSSKMPGYVYVLGENPYFMNQKMPKIAXXXXXXXXXIGTKWMEAEREFQALVGLEGDYLGPIS 288
            FK+F+GE+L+GVVHKVLKHGV +R GP+EN YLS  KMP Y YV GENP F+N+K+ KI          +GTKW+EAEREFQALV LEGDYLGP+S
Sbjct:  146 FKLFRGEILDGVVHKVLKHGVILRCGPVENIYLSCQKMPDYRYVPGENPVFLNEKLSKIEKDVVVRFIVMGTKWLEAEREFQALVSLEGDYLGPLS 241

[Close]

BLAST of HO063997 vs. TrEMBL
Match: B9HUM9_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_566143 PE=4 SV=1)

HSP 1 Score: 137.502 bits (345), Expect = 4.189e-31
Identity = 67/96 (69.79%), Postives = 73/96 (76.04%), Query Frame = 1

Query:    1 FKIFKGEVLEGVVHKVLKHGVFMRIGPIENAYLSSSKMPGYVYVLGENPYFMNQKMPKIAXXXXXXXXXIGTKWMEAEREFQALVGLEGDYLGPIS 288
            FKIFKGE+LEGVVHKVLKHGV +R GPIEN YLSS KM  Y YV GENP F+N K  KI          +GTKW+EAEREFQALV LEGDYLGP+S
Sbjct:   98 FKIFKGEILEGVVHKVLKHGVLLRCGPIENIYLSSMKMLDYRYVPGENPVFLNDKTSKIEKDVVVRFVVLGTKWLEAEREFQALVSLEGDYLGPVS 193

[Close]

BLAST of HO063997 vs. TrEMBL
Match: A9PGH2_POPTR (Putative uncharacterized protein OS=Populus trichocarpa PE=2 SV=1)

HSP 1 Score: 137.502 bits (345), Expect = 4.189e-31
Identity = 67/96 (69.79%), Postives = 73/96 (76.04%), Query Frame = 1

Query:    1 FKIFKGEVLEGVVHKVLKHGVFMRIGPIENAYLSSSKMPGYVYVLGENPYFMNQKMPKIAXXXXXXXXXIGTKWMEAEREFQALVGLEGDYLGPIS 288
            FKIFKGE+LEGVVHKVLKHGV +R GPIEN YLSS KM  Y YV GENP F+N K  KI          +GTKW+EAEREFQALV LEGDYLGP+S
Sbjct:   81 FKIFKGEILEGVVHKVLKHGVLLRCGPIENIYLSSMKMLDYRYVPGENPVFLNDKTSKIEKDVVVRFVVLGTKWLEAEREFQALVSLEGDYLGPVS 176

[Close]

BLAST of HO063997 vs. TrEMBL
Match: A5BM26_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_043525 PE=4 SV=1)

HSP 1 Score: 137.502 bits (345), Expect = 4.189e-31
Identity = 63/96 (65.62%), Postives = 75/96 (78.12%), Query Frame = 1

Query:    1 FKIFKGEVLEGVVHKVLKHGVFMRIGPIENAYLSSSKMPGYVYVLGENPYFMNQKMPKIAXXXXXXXXXIGTKWMEAEREFQALVGLEGDYLGPIS 288
            FK+F+GE+L+GVVHKVLKHGV +R GP+EN YLS  KMP Y YV GENP F+N+K+ KI          +GTKW+EAEREFQALV LEGDYLGP+S
Sbjct:   81 FKLFRGEILDGVVHKVLKHGVILRCGPVENIYLSCQKMPDYRYVPGENPVFLNEKLSKIEKDVVVRFIVMGTKWLEAEREFQALVSLEGDYLGPLS 176

[Close]

BLAST of HO063997 vs. TAIR peptide
Match: AT4G14660.1 (| Symbols: NRPE7 | RNA polymerase Rpb7-like, N-terminal domain | chr4:8406500-8407036 REVERSE LENGTH=178)

HSP 1 Score: 137.887 bits (346), Expect = 2.477e-33
Identity = 65/96 (67.71%), Postives = 72/96 (75.00%), Query Frame = 1

Query:    1 FKIFKGEVLEGVVHKVLKHGVFMRIGPIENAYLSSSKMPGYVYVLGENPYFMNQKMPKIAXXXXXXXXXIGTKWMEAEREFQALVGLEGDYLGPIS 288
            FKIFKGE++ GVVHKVLKHGVFMR GPIEN YLS +KMP Y Y+ GENP FMN+K  +I          IG KWME EREFQAL  LEGDYLGP+S
Sbjct:   81 FKIFKGEIIHGVVHKVLKHGVFMRCGPIENVYLSYTKMPDYKYIPGENPIFMNEKTSRIQVETTVRVVVIGIKWMEVEREFQALASLEGDYLGPLS 176

[Close]

BLAST of HO063997 vs. TAIR peptide
Match: AT3G22900.1 (| Symbols: NRPD7 | RNA polymerase Rpb7-like, N-terminal domain | chr3:8115645-8116169 FORWARD LENGTH=174)

HSP 1 Score: 108.997 bits (271), Expect = 1.232e-24
Identity = 50/94 (53.19%), Postives = 65/94 (69.15%), Query Frame = 1

Query:    1 FKIFKGEVLEGVVHKVLKHGVFMRIGPIENAYLSSSKMPGYVYVLGENPYFMNQKMPKIAXXXXXXXXXIGTKWMEAEREFQALVGLEGDYLGP 282
            FK+FKGE++ GVVHKV K GVF++ GP E  YLS  KMPGY ++ GENP+FMNQ M +I          + T+W EAE++F AL  ++GD LGP
Sbjct:   80 FKMFKGEIVHGVVHKVHKTGVFLKSGPYEIIYLSHMKMPGYEFIPGENPFFMNQYMSRIQIGARVRFVVLDTEWREAEKDFMALASIDGDNLGP 173

[Close]

BLAST of HO063997 vs. TAIR peptide
Match: AT4G14520.3 (| Symbols: | DNA-directed RNA polymerase II-related | chr4:8342471-8343073 FORWARD LENGTH=200)

HSP 1 Score: 58.5362 bits (140), Expect = 1.910e-9
Identity = 37/102 (36.27%), Postives = 52/102 (50.98%), Query Frame = 1

Query:   13 KGEVLEGVVHKVLKHGVFMRIGPIENAYLSSSKMPGYVYVLG-----ENPYFMNQKMPKIAXXXXXXXXXIGTKWME----AEREFQALVGLEGD-YLGPIS 288
            +G++L+G V KVL +G F+R GP+  AYLS  KMP Y YV       E P+F    + KIA         +  ++ E       ++  L  LEG+   GPIS
Sbjct:   90 RGDILQGTVKKVLWNGAFIRSGPLRYAYLSLLKMPHYHYVHSPLSEDEKPHFQKDDLSKIAVGVVVRFQVLAVRFKERPHKRRNDYYVLATLEGNGSFGPIS 191

[Close]

BLAST of HO063997 vs. TAIR peptide
Match: AT4G14520.2 (| Symbols: | DNA-directed RNA polymerase II-related | chr4:8342471-8343073 FORWARD LENGTH=200)

HSP 1 Score: 58.5362 bits (140), Expect = 1.910e-9
Identity = 37/102 (36.27%), Postives = 52/102 (50.98%), Query Frame = 1

Query:   13 KGEVLEGVVHKVLKHGVFMRIGPIENAYLSSSKMPGYVYVLG-----ENPYFMNQKMPKIAXXXXXXXXXIGTKWME----AEREFQALVGLEGD-YLGPIS 288
            +G++L+G V KVL +G F+R GP+  AYLS  KMP Y YV       E P+F    + KIA         +  ++ E       ++  L  LEG+   GPIS
Sbjct:   90 RGDILQGTVKKVLWNGAFIRSGPLRYAYLSLLKMPHYHYVHSPLSEDEKPHFQKDDLSKIAVGVVVRFQVLAVRFKERPHKRRNDYYVLATLEGNGSFGPIS 191

[Close]

BLAST of HO063997 vs. TAIR peptide
Match: AT4G14520.1 (| Symbols: | DNA-directed RNA polymerase II-related | chr4:8342471-8343073 FORWARD LENGTH=200)

HSP 1 Score: 58.5362 bits (140), Expect = 1.910e-9
Identity = 37/102 (36.27%), Postives = 52/102 (50.98%), Query Frame = 1

Query:   13 KGEVLEGVVHKVLKHGVFMRIGPIENAYLSSSKMPGYVYVLG-----ENPYFMNQKMPKIAXXXXXXXXXIGTKWME----AEREFQALVGLEGD-YLGPIS 288
            +G++L+G V KVL +G F+R GP+  AYLS  KMP Y YV       E P+F    + KIA         +  ++ E       ++  L  LEG+   GPIS
Sbjct:   90 RGDILQGTVKKVLWNGAFIRSGPLRYAYLSLLKMPHYHYVHSPLSEDEKPHFQKDDLSKIAVGVVVRFQVLAVRFKERPHKRRNDYYVLATLEGNGSFGPIS 191

The following BLAST results are available for this feature:

BLAST of HO063997 vs. TrEMBL
BLAST of HO063997 vs. TAIR peptide

BLAST of HO063997 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
B7FLW9_MEDTR	1.341e-45	91.00	Putative uncharacterized protein OS=Medicago trunc...	[more]
C6T5N3_SOYBN	4.782e-35	74.00	Putative uncharacterized protein OS=Glycine max PE...	[more]
B9S9D8_RICCO	3.789e-32	70.83	DNA-directed RNA polymerase II 19 kD polypeptide r...	[more]
D7MGZ2_ARALY	8.441e-32	69.79	RNA polymerase Rpb7 N-terminal domain-containing p...	[more]
O23322_ARATH	3.208e-31	67.71	RNA polymerase II fifth largest subunit like prote...	[more]
A6QRA1_ARATH	3.208e-31	67.71	At4g14660 OS=Arabidopsis thaliana GN=At4g14660 PE=...	[more]
D7T792_VITVI	4.189e-31	65.63	Whole genome shotgun sequence of line PN40024, sca...	[more]
B9HUM9_POPTR	4.189e-31	69.79	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
A9PGH2_POPTR	4.189e-31	69.79	Putative uncharacterized protein OS=Populus tricho...	[more]
A5BM26_VITVI	4.189e-31	65.63	Putative uncharacterized protein OS=Vitis vinifera...	[more]

BLAST of HO063997 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide)
Total hits: 5


Match Name	E-value	Identity	Description
AT4G14660.1	2.477e-33	67.71	\| Symbols: NRPE7 \| RNA polymerase Rpb7-like, N-ter...	[more]
AT3G22900.1	1.232e-24	53.19	\| Symbols: NRPD7 \| RNA polymerase Rpb7-like, N-ter...	[more]
AT4G14520.3	1.910e-9	36.27	\| Symbols: \| DNA-directed RNA polymerase II-relat...	[more]
AT4G14520.2	1.910e-9	36.27	\| Symbols: \| DNA-directed RNA polymerase II-relat...	[more]
AT4G14520.1	1.910e-9	36.27	\| Symbols: \| DNA-directed RNA polymerase II-relat...	[more]

InterPro

Analysis Name: InterProScan analysis for Cicer arietinum unigene v1
Date Performed: 2010-12-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR003029	Ribosomal protein S1, RNA-binding domain	PFAM	PF00575	S1	coord: 4..39 score: 8.1
IPR003029	Ribosomal protein S1, RNA-binding domain	PROFILE	PS50126	S1	coord: 6..88 score: 9
IPR012340	Nucleic acid-binding, OB-fold	GENE3D	2.40.50.140	no description	coord: 7..95 score: 8
IPR016027	Nucleic acid-binding, OB-fold-like	SUPERFAMILY	50249	Nucleic acid-binding proteins	coord: 3..95 score: 1.1
None	No IPR available	PANTHER	PTHR12709	DNA-DIRECTED RNA POLYMERASE II, III	coord: 1..100 score: 2.3
None	No IPR available	PANTHER	PTHR12709:SF1	DNA-DIRECTED RNA POLYMERASE II 19 KDA POLYPEPTIDE RPB7	coord: 1..100 score: 2.3
None	No IPR available	SEG	seg	seg	coord: 61..69 score
None	No IPR available	SEG	seg	seg	coord: 13..24 score

Properties


Property Name	Value
Singlet in analysis	Cicer arietinum unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>HO063997 ID=HO063997; Name=HO063997; organism=Cicer arietinum; type=EST; length=469bp
TTTAAGATTTTCAAGGGTGAGGTATTGGAAGGTGTGGTCCACAAAGTTCT
GAAGCATGGTGTTTTCATGAGAATTGGTCCAATTGAGAATGCCTATCTCT
CGAGTAGCAAGATGCCTGGTTACGTGTATGTTCTCGGTGAAAACCCATAC
TTCATGAATCAGAAAATGCCTAAGATTGCAAAANACGTCAAAGTTCGAGT
TGTTGTGATTGGTACAAAGTGGATGGAGGCAGAGAGGGAGTTTCAGGCAC
TGGTCGGTTTGGAAGGAGATTATCTGGGACCAATTTCATCCCCTGATATG
TAGCTGCATTTTCTTGTAACTAAACATTGCAGTGTAAGTTTGTTAGTGAA
AGCAACCACATTTTGCATCAGGGCTTCATATTTTGCTTTCCGGGTGGGCA
AATCATGGAACCATCAATCAAACTAATGCAGTGGGGTTTAGTCTGTAAAT
AAATACCAGATGTGTACCT

Annotated Terms

The following terms have been associated with this EST:

Vocabulary: **Molecular Function**
Term	Definition
GO:0003723	RNA binding

Vocabulary: **INTERPRO**
Term	Definition
IPR003029	S1_domain
IPR012340	NA-bd_OB-fold
IPR016027	Nucleic acid-binding, OB-fold-like

Search form