HO064114

EST Overview


Name	HO064114
Unique Name	HO064114
Type	EST
Organism	Cicer arietinum (chickpea)
Sequence length	478

Libraries


Library Name	Type
LIBEST_026411 Chickpea drought stressed cDNA SSH library AR2	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Cicer arietinum unigene v1	Cicer arietinum unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Cicer arietinum unigene v1	2010-12-27
Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide	2011-02-03
Homology Analysis: Cicer arietinum unigene v1 vs Trembl	2010-12-28
InterProScan analysis for Cicer arietinum unigene v1	2010-12-29

Homology

[Close]

BLAST of HO064114 vs. TrEMBL
Match: B9RD96_RICCO (Oxidoreductase, putative OS=Ricinus communis GN=RCOM_1611210 PE=4 SV=1)

HSP 1 Score: 156.762 bits (395), Expect = 3.043e-48
Identity = 74/90 (82.22%), Postives = 82/90 (91.11%), Query Frame = 3

Query:  102 NSLPSPKPISKTTISQFLYDSLSLSAWKSTSFSTWSLRVNPSSGNLHPTEAYIVAPSIESISDSPFVAHYAPKEHSLELRAQIPSGFFPK 371
            NSLPSPK ISK++ISQ  Y+SL+LSAWK+T FSTWSLRVNPSSGNLHPTEAY++AP IESISDS FV+HYAPKEHSLELRA IPS FFPK
Sbjct:  134 NSLPSPKSISKSSISQLFYNSLALSAWKTTGFSTWSLRVNPSSGNLHPTEAYLIAPPIESISDSAFVSHYAPKEHSLELRATIPSNFFPK 223

HSP 2 Score: 59.3066 bits (142), Expect = 3.043e-48
Identity = 24/32 (75.00%), Postives = 27/32 (84.38%), Query Frame = 2

Query:  362 FSQIFPPNSFLVGFSSIFWRESWKYGERGFRY 457
            F + FP NSFL+G SSIFWRE+WKYGER FRY
Sbjct:  221 FPKYFPRNSFLIGISSIFWREAWKYGERAFRY 252

[Close]

BLAST of HO064114 vs. TrEMBL
Match: B9GPE5_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_755156 PE=4 SV=1)

HSP 1 Score: 153.68 bits (387), Expect = 2.111e-46
Identity = 70/89 (78.65%), Postives = 81/89 (91.01%), Query Frame = 3

Query:  102 NSLPSPKPISKTTISQFLYDSLSLSAWKSTSFSTWSLRVNPSSGNLHPTEAYIVAPSIESISDSPFVAHYAPKEHSLELRAQIPSGFFP 368
            NSLPSPKPISK++ISQ  YDSL+LSAWK+T FSTWSLRVNPSSGNLHPTEAYI++P+++S+ DS FVAHYAPKEHSLELRA+IP  F P
Sbjct:   93 NSLPSPKPISKSSISQLFYDSLALSAWKTTGFSTWSLRVNPSSGNLHPTEAYIISPAVDSVCDSAFVAHYAPKEHSLELRAKIPDTFLP 181

HSP 2 Score: 56.225 bits (134), Expect = 2.111e-46
Identity = 22/28 (78.57%), Postives = 25/28 (89.29%), Query Frame = 2

Query:  374 FPPNSFLVGFSSIFWRESWKYGERGFRY 457
            FP N+FL+G SSIFWRE+WKYGER FRY
Sbjct:  184 FPSNAFLIGVSSIFWREAWKYGERAFRY 211

[Close]

BLAST of HO064114 vs. TrEMBL
Match: D7KP50_ARALY (Putative uncharacterized protein OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_333455 PE=4 SV=1)

HSP 1 Score: 140.198 bits (352), Expect = 2.681e-43
Identity = 66/90 (73.33%), Postives = 74/90 (82.22%), Query Frame = 3

Query:  102 NSLPSPKPISKTTISQFLYDSLSLSAWKSTSFSTWSLRVNPSSGNLHPTEAYIVAPSIESISDSPFVAHYAPKEHSLELRAQIPSGFFPK 371
            +SLP PKPIS  TIS   Y SL+LSAWK+T  STW LRVNPSSGNLHPTEAY++AP I S+S S FVAHYAPKEHSLE+RA IPS FFP+
Sbjct:  368 DSLPPPKPISLATISHLFYHSLALSAWKTTGSSTWPLRVNPSSGNLHPTEAYLIAPPIPSLSQSAFVAHYAPKEHSLEVRAHIPSSFFPE 457

HSP 2 Score: 59.3066 bits (142), Expect = 2.681e-43
Identity = 24/31 (77.42%), Postives = 26/31 (83.87%), Query Frame = 2

Query:  365 SQIFPPNSFLVGFSSIFWRESWKYGERGFRY 457
            S  FP NSFL+G SSIFWRE+WKYGER FRY
Sbjct:  452 SSFFPENSFLIGISSIFWREAWKYGERAFRY 482

[Close]

BLAST of HO064114 vs. TrEMBL
Match: O23673_ARATH (Putative uncharacterized protein At1g02020 OS=Arabidopsis thaliana GN=At1g02020 PE=2 SV=1)

HSP 1 Score: 138.272 bits (347), Expect = 5.952e-43
Identity = 65/89 (73.03%), Postives = 73/89 (82.02%), Query Frame = 3

Query:  102 NSLPSPKPISKTTISQFLYDSLSLSAWKSTSFSTWSLRVNPSSGNLHPTEAYIVAPSIESISDSPFVAHYAPKEHSLELRAQIPSGFFP 368
            +SLP PKPIS  TIS   Y SL+LSAWK+T  STW LRVNPSSGNLHPTEAY++AP I S+S S FV+HYAPKEHSLE+RA IPS FFP
Sbjct:  134 DSLPPPKPISLPTISHLFYHSLALSAWKTTGSSTWPLRVNPSSGNLHPTEAYLIAPPIPSLSQSAFVSHYAPKEHSLEVRAHIPSSFFP 222

HSP 2 Score: 60.077 bits (144), Expect = 5.952e-43
Identity = 25/36 (69.44%), Postives = 27/36 (75.00%), Query Frame = 2

Query:  350 PIWVFSQIFPPNSFLVGFSSIFWRESWKYGERGFRY 457
            P   F   FP NSFL+G SSIFWRE+WKYGER FRY
Sbjct:  217 PSSFFPNFFPENSFLIGISSIFWREAWKYGERAFRY 252

[Close]

BLAST of HO064114 vs. TrEMBL
Match: Q3E6Y8_ARATH (Uncharacterized protein At1g02020.2 OS=Arabidopsis thaliana GN=At1g02020 PE=4 SV=1)

HSP 1 Score: 138.272 bits (347), Expect = 5.962e-43
Identity = 65/89 (73.03%), Postives = 73/89 (82.02%), Query Frame = 3

Query:  102 NSLPSPKPISKTTISQFLYDSLSLSAWKSTSFSTWSLRVNPSSGNLHPTEAYIVAPSIESISDSPFVAHYAPKEHSLELRAQIPSGFFP 368
            +SLP PKPIS  TIS   Y SL+LSAWK+T  STW LRVNPSSGNLHPTEAY++AP I S+S S FV+HYAPKEHSLE+RA IPS FFP
Sbjct:  134 DSLPPPKPISLPTISHLFYHSLALSAWKTTGSSTWPLRVNPSSGNLHPTEAYLIAPPIPSLSQSAFVSHYAPKEHSLEVRAHIPSSFFP 222

HSP 2 Score: 60.077 bits (144), Expect = 5.962e-43
Identity = 25/36 (69.44%), Postives = 27/36 (75.00%), Query Frame = 2

Query:  350 PIWVFSQIFPPNSFLVGFSSIFWRESWKYGERGFRY 457
            P   F   FP NSFL+G SSIFWRE+WKYGER FRY
Sbjct:  217 PSSFFPNFFPENSFLIGISSIFWREAWKYGERAFRY 252

[Close]

BLAST of HO064114 vs. TrEMBL
Match: A9RNQ9_PHYPA (Predicted protein (Fragment) OS=Physcomitrella patens subsp. patens GN=PHYPADRAFT_21437 PE=4 SV=1)

HSP 1 Score: 119.783 bits (299), Expect = 3.590e-36
Identity = 53/79 (67.09%), Postives = 67/79 (84.81%), Query Frame = 3

Query:  114 SPKPISKTTISQFLYDSLSLSAWKSTSFSTWSLRVNPSSGNLHPTEAYIVAPSIESISDSPFVAHYAPKEHSLELRAQI 350
            +PKP+ K+++SQ LYDSL+LSAWK+   STWSLRVNPSSGNLHPTE Y++  +IE +S +PFVAHYAP EH LE+RA+I
Sbjct:   70 APKPLDKSSLSQLLYDSLALSAWKAARRSTWSLRVNPSSGNLHPTEGYVICDAIEGVSMTPFVAHYAPYEHVLEIRAEI 148

HSP 2 Score: 55.8398 bits (133), Expect = 3.590e-36
Identity = 23/27 (85.19%), Postives = 23/27 (85.19%), Query Frame = 2

Query:  377 PPNSFLVGFSSIFWRESWKYGERGFRY 457
            PP S L GFSSIFWRESWKYGER FRY
Sbjct:  161 PPGSLLFGFSSIFWRESWKYGERAFRY 187

[Close]

BLAST of HO064114 vs. TrEMBL
Match: D7U7W6_VITVI (Whole genome shotgun sequence of line PN40024, scaffold_48.assembly12x (Fragment) OS=Vitis vinifera GN=VIT_00027661001 PE=4 SV=1)

HSP 1 Score: 137.117 bits (344), Expect = 2.663e-33
Identity = 62/77 (80.52%), Postives = 72/77 (93.51%), Query Frame = 3

Query:  105 SLPSPKPISKTTISQFLYDSLSLSAWKSTSFSTWSLRVNPSSGNLHPTEAYIVAPSIESISDSPFVAHYAPKEHSLE 335
            +LP PKPISK+TISQ  +DSL++SAWK+T FSTWSLRVNPSSGNLHPTE+YI+AP+IES+SDS FVAHYAPKEHSLE
Sbjct:   82 NLPPPKPISKSTISQLFFDSLAISAWKTTGFSTWSLRVNPSSGNLHPTESYIIAPAIESVSDSAFVAHYAPKEHSLE 158

HSP 2 Score: 28.8758 bits (63), Expect = 2.663e-33
Identity = 10/12 (83.33%), Postives = 11/12 (91.67%), Query Frame = 2

Query:  422 ESWKYGERGFRY 457
            E+WKYGER FRY
Sbjct:  158 EAWKYGERAFRY 169

[Close]

BLAST of HO064114 vs. TrEMBL
Match: A4XYQ2_PSEMY (Putative uncharacterized protein OS=Pseudomonas mendocina (strain ymp) GN=Pmen_3720 PE=4 SV=1)

HSP 1 Score: 98.2117 bits (243), Expect = 6.531e-28
Identity = 41/80 (51.25%), Postives = 63/80 (78.75%), Query Frame = 3

Query:  117 PKPISKTTISQFLYDSLSLSAWKSTSFSTWSLRVNPSSGNLHPTEAYIVAPSIESISDSPFVAHYAPKEHSLELRAQIPS 356
            P P+++ ++SQ LYDSL++SAWK    + W+LRVNPSSGNLHPTEAY++ P  +++ +   +AHYAP  H+LE+R+++P+
Sbjct:   67 PAPLNRASVSQLLYDSLAISAWKEAGGNRWALRVNPSSGNLHPTEAYLLLPP-DAVDELAVLAHYAPDVHALEVRSELPA 145

HSP 2 Score: 49.6766 bits (117), Expect = 6.531e-28
Identity = 19/27 (70.37%), Postives = 22/27 (81.48%), Query Frame = 2

Query:  377 PPNSFLVGFSSIFWRESWKYGERGFRY 457
            PP  FL+G SSI WRE+WKYGER +RY
Sbjct:  156 PPGGFLLGLSSIAWREAWKYGERAYRY 182

[Close]

BLAST of HO064114 vs. TrEMBL
Match: B8BG17_ORYSI (Putative uncharacterized protein OS=Oryza sativa subsp. indica GN=OsI_32957 PE=4 SV=1)

HSP 1 Score: 94.7449 bits (234), Expect = 7.514e-26
Identity = 48/88 (54.55%), Postives = 62/88 (70.45%), Query Frame = 3

Query:  111 PSPKPISKTTISQFLYDSLSLSAWKSTSFSTWSLRVNPSSGNLHPTEAYIVAPSIESISDSP--FVAHYAPKEHSLELRAQIPSGFFP 368
            PSP+P+S  ++S  L+ SL+LSAWKS   STWSLRVNPSSGNLHPTEA+++    E   D     V+HYAP++H LE+R+  P   FP
Sbjct:  106 PSPQPLSVDSLSALLFHSLALSAWKSAGVSTWSLRVNPSSGNLHPTEAHLL---FEHPRDPGRLVVSHYAPRDHLLEVRSAAPLADFP 190

HSP 2 Score: 46.2098 bits (108), Expect = 7.514e-26
Identity = 22/40 (55.00%), Postives = 26/40 (65.00%), Query Frame = 2

Query:  341 SSNPIWVFSQIFPPNSFLV-GFSSIFWRESWKYGERGFRY 457
            S+ P+  F  + PP +  V   SSIFWRESWKYGER  RY
Sbjct:  182 SAAPLADFPALLPPPATAVLALSSIFWRESWKYGERALRY 221

[Close]

BLAST of HO064114 vs. TrEMBL
Match: Q8LMV1_ORYSJ (Nitroreductase family protein, putative OS=Oryza sativa subsp. japonica GN=OSJNBa0040E17.29 PE=4 SV=1)

HSP 1 Score: 94.7449 bits (234), Expect = 7.519e-26
Identity = 48/88 (54.55%), Postives = 62/88 (70.45%), Query Frame = 3

Query:  111 PSPKPISKTTISQFLYDSLSLSAWKSTSFSTWSLRVNPSSGNLHPTEAYIVAPSIESISDSP--FVAHYAPKEHSLELRAQIPSGFFP 368
            PSP+P+S  ++S  L+ SL+LSAWKS   STWSLRVNPSSGNLHPTEA+++    E   D     V+HYAP++H LE+R+  P   FP
Sbjct:   81 PSPQPLSVDSLSALLFHSLALSAWKSAGVSTWSLRVNPSSGNLHPTEAHLL---FEHPRDPGRLVVSHYAPRDHLLEVRSAAPLADFP 165

HSP 2 Score: 46.2098 bits (108), Expect = 7.519e-26
Identity = 22/40 (55.00%), Postives = 26/40 (65.00%), Query Frame = 2

Query:  341 SSNPIWVFSQIFPPNSFLV-GFSSIFWRESWKYGERGFRY 457
            S+ P+  F  + PP +  V   SSIFWRESWKYGER  RY
Sbjct:  157 SAAPLADFPALLPPPATAVLALSSIFWRESWKYGERALRY 196

[Close]

BLAST of HO064114 vs. TAIR peptide
Match: AT1G02020.1 (| Symbols: | nitroreductase family protein | chr1:352842-354845 REVERSE LENGTH=642)

HSP 1 Score: 138.272 bits (347), Expect = 4.764e-45
Identity = 65/89 (73.03%), Postives = 73/89 (82.02%), Query Frame = 3

Query:  102 NSLPSPKPISKTTISQFLYDSLSLSAWKSTSFSTWSLRVNPSSGNLHPTEAYIVAPSIESISDSPFVAHYAPKEHSLELRAQIPSGFFP 368
            +SLP PKPIS  TIS   Y SL+LSAWK+T  STW LRVNPSSGNLHPTEAY++AP I S+S S FV+HYAPKEHSLE+RA IPS FFP
Sbjct:  134 DSLPPPKPISLPTISHLFYHSLALSAWKTTGSSTWPLRVNPSSGNLHPTEAYLIAPPIPSLSQSAFVSHYAPKEHSLEVRAHIPSSFFP 222

HSP 2 Score: 60.077 bits (144), Expect = 4.764e-45
Identity = 25/36 (69.44%), Postives = 27/36 (75.00%), Query Frame = 2

Query:  350 PIWVFSQIFPPNSFLVGFSSIFWRESWKYGERGFRY 457
            P   F   FP NSFL+G SSIFWRE+WKYGER FRY
Sbjct:  217 PSSFFPNFFPENSFLIGISSIFWREAWKYGERAFRY 252

[Close]

BLAST of HO064114 vs. TAIR peptide
Match: AT1G02020.2 (| Symbols: | nitroreductase family protein | chr1:353214-354845 REVERSE LENGTH=543)

HSP 1 Score: 138.272 bits (347), Expect = 4.772e-45
Identity = 65/89 (73.03%), Postives = 73/89 (82.02%), Query Frame = 3

Query:  102 NSLPSPKPISKTTISQFLYDSLSLSAWKSTSFSTWSLRVNPSSGNLHPTEAYIVAPSIESISDSPFVAHYAPKEHSLELRAQIPSGFFP 368
            +SLP PKPIS  TIS   Y SL+LSAWK+T  STW LRVNPSSGNLHPTEAY++AP I S+S S FV+HYAPKEHSLE+RA IPS FFP
Sbjct:  134 DSLPPPKPISLPTISHLFYHSLALSAWKTTGSSTWPLRVNPSSGNLHPTEAYLIAPPIPSLSQSAFVSHYAPKEHSLEVRAHIPSSFFP 222

HSP 2 Score: 60.077 bits (144), Expect = 4.772e-45
Identity = 25/36 (69.44%), Postives = 27/36 (75.00%), Query Frame = 2

Query:  350 PIWVFSQIFPPNSFLVGFSSIFWRESWKYGERGFRY 457
            P   F   FP NSFL+G SSIFWRE+WKYGER FRY
Sbjct:  217 PSSFFPNFFPENSFLIGISSIFWREAWKYGERAFRY 252

The following BLAST results are available for this feature:

BLAST of HO064114 vs. TrEMBL
BLAST of HO064114 vs. TAIR peptide

BLAST of HO064114 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
B9RD96_RICCO	3.043e-48	82.22	Oxidoreductase, putative OS=Ricinus communis GN=RC...	[more]
B9GPE5_POPTR	2.111e-46	78.65	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
D7KP50_ARALY	2.681e-43	73.33	Putative uncharacterized protein OS=Arabidopsis ly...	[more]
O23673_ARATH	5.952e-43	73.03	Putative uncharacterized protein At1g02020 OS=Arab...	[more]
Q3E6Y8_ARATH	5.962e-43	73.03	Uncharacterized protein At1g02020.2 OS=Arabidopsis...	[more]
A9RNQ9_PHYPA	3.590e-36	67.09	Predicted protein (Fragment) OS=Physcomitrella pat...	[more]
D7U7W6_VITVI	2.663e-33	80.52	Whole genome shotgun sequence of line PN40024, sca...	[more]
A4XYQ2_PSEMY	6.531e-28	51.25	Putative uncharacterized protein OS=Pseudomonas me...	[more]
B8BG17_ORYSI	7.514e-26	54.55	Putative uncharacterized protein OS=Oryza sativa s...	[more]
Q8LMV1_ORYSJ	7.519e-26	54.55	Nitroreductase family protein, putative OS=Oryza s...	[more]

BLAST of HO064114 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide)
Total hits: 2


Match Name	E-value	Identity	Description
AT1G02020.1	4.764e-45	73.03	\| Symbols: \| nitroreductase family protein \| chr1...	[more]
AT1G02020.2	4.772e-45	73.03	\| Symbols: \| nitroreductase family protein \| chr1...	[more]

InterPro

Analysis Name: InterProScan analysis for Cicer arietinum unigene v1
Date Performed: 2010-12-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	SEG	seg	seg	coord: 7..33 score
None	No IPR available	SEG	seg	seg	coord: 14..39 score
None	No IPR available	SEG	seg	seg	coord: 10..18 score: NAcoord: 21..28 score
None	No IPR available	SEG	seg	seg	coord: 8..33 score: NAcoord: 124..137 score
None	No IPR available	SEG	seg	seg	coord: 30..35 score: NAcoord: 40..55 score

Properties


Property Name	Value
Singlet in analysis	Cicer arietinum unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>HO064114 ID=HO064114; Name=HO064114; organism=Cicer arietinum; type=EST; length=478bp
TAGCGTGGTCGCGGCCGAGGTACCTCTCTTCTCCTCTTCTCCCTCTTCTC
CATTTCACTACACAACAACAACAGCAGCAACCACTTTACTCTTCTCTCTT
CAATTCTCTTCCATCCCCAAAACCCATTTCCAAAACCACTATTTCTCAGT
TTCTCTATGATTCTCTCTCACTCTCAGCTTGGAAATCCACAAGCTTTTCA
ACTTGGTCTTTAAGGGTTAACCCTAGTAGTGGTAATTTGCACCCAACTGA
AGCTTATATCGTTGCACCTTCAATTGAATCAATCTCTGATTCACCTTTTG
TTGCTCATTATGCTCCAAAGGAACATTCTTTGGAACTTAGAGCTCAAATC
CCATCTGGGTTTTTTCCCAAATTTTTCCACCTAATTCTTTTCTTGTTGGG
TTTTCTTCCATTTTCTGGCGTGAATCTTGGAAGTATGGTGAACGTGGTTT
TAGGTACCTGCCCGGGCGGCCGCTCGAA

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