HO067986

EST Overview


Name	HO067986
Unique Name	HO067986
Type	EST
Organism	Cicer arietinum (chickpea)
Sequence length	640

Libraries


Library Name	Type
LIBEST_026415 Chickpea drought stressed cDNA SSH library BULK2	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Cicer arietinum unigene v1	Cicer arietinum unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Cicer arietinum unigene v1	2010-12-27
KEGG analysis for Cicer arietinum unigene v1	2011-01-05
Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide	2011-02-03
Homology Analysis: Cicer arietinum unigene v1 vs Swissprot	2010-12-28
Homology Analysis: Cicer arietinum unigene v1 vs Trembl	2010-12-28
InterProScan analysis for Cicer arietinum unigene v1	2010-12-29

Homology

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BLAST of HO067986 vs. SwissProt
Match: GYRA_NICBE (DNA gyrase subunit A, chloroplastic/mitochondrial OS=Nicotiana benthamiana GN=GYRA PE=2 SV=1)

HSP 1 Score: 290.812 bits (743), Expect = 1.422e-83
Identity = 146/195 (74.87%), Postives = 172/195 (88.21%), Query Frame = 1

Query:    1 ARFQLSKALQRRHVVEGILVGFNNLDRVIRIIREASSNTIAAAGLRNEFNLSEKQAEALLDMSLRRLTLRESDNFVAENKSLVEQISKLEELLSSRKNILELIEQEAIDLKNKFASPRRSILEDTDNGQLDDIDVIPNEEMLLALSEKGYLKRMKPSTFNLQNRGTIGKSVGKLKMNDSMSDFIVCRAHDYVLYF 585
            ARF+LS+A +R H+VEGI+VG +NLD VI  IR+ASSN +AAA LR EF LSEKQAEA+LD+SLRRLT  E + FV E KSL  QISKLEELLSS+K IL+LIE+EAI++KNKF +PRRS+LEDTD+G L+DIDVIPNEEMLLA+SEKGY+KRMKP TFNLQNRGTIGKSVGKL++ND+MSDF+VCRAHD VLYF
Sbjct:  450 ARFKLSQAQERNHIVEGIIVGLDNLDEVINTIRKASSNALAAASLRKEFELSEKQAEAILDISLRRLTALERNKFVEEGKSLRTQISKLEELLSSKKQILQLIEEEAIEIKNKFFNPRRSMLEDTDSGDLEDIDVIPNEEMLLAISEKGYVKRMKPDTFNLQNRGTIGKSVGKLRVNDAMSDFLVCRAHDKVLYF 644

HSP 2 Score: 40.4318 bits (93), Expect = 1.422e-83
Identity = 20/21 (95.24%), Postives = 20/21 (95.24%), Query Frame = 3

Query:  576 PLFQILSLSDGERITSIIPVS 638
            PL QILSLSDGERITSIIPVS
Sbjct:  669 PLVQILSLSDGERITSIIPVS 689

[Close]

BLAST of HO067986 vs. SwissProt
Match: GYRA_ARATH (Probable DNA gyrase subunit A, chloroplastic/mitochondrial OS=Arabidopsis thaliana GN=GYRA PE=2 SV=2)

HSP 1 Score: 265.388 bits (677), Expect = 4.905e-75
Identity = 129/195 (66.15%), Postives = 171/195 (87.69%), Query Frame = 1

Query:    1 ARFQLSKALQRRHVVEGILVGFNNLDRVIRIIREASSNTIAAAGLRNEFNLSEKQAEALLDMSLRRLTLRESDNFVAENKSLVEQISKLEELLSSRKNILELIEQEAIDLKNKFASPRRSILEDTDNGQLDDIDVIPNEEMLLALSEKGYLKRMKPSTFNLQNRGTIGKSVGKLKMNDSMSDFIVCRAHDYVLYF 585
            ARF+LS A QR+H++EGI+VG +N+D VI +I +ASS++ A A L++E+ LSEKQAEA+L+++LRRLT  E   F  E+ SL EQI+KLE+LLS+R NIL+LIEQEAI+LK++F+SPRRS+LED+D+G L+DIDVIPNEEML+A+SEKGY+KRMK  TFNLQ+RGTIGKSVGKL+++D+MSDF+VC AHD+VL+F
Sbjct:  461 ARFKLSHAQQRKHIIEGIVVGLDNVDEVIELITKASSHSSATAALQSEYGLSEKQAEAILEITLRRLTALERKKFTDESSSLTEQITKLEQLLSTRTNILKLIEQEAIELKDRFSSPRRSMLEDSDSGDLEDIDVIPNEEMLMAVSEKGYVKRMKADTFNLQHRGTIGKSVGKLRVDDAMSDFLVCHAHDHVLFF 655

HSP 2 Score: 37.3502 bits (85), Expect = 4.905e-75
Identity = 16/21 (76.19%), Postives = 20/21 (95.24%), Query Frame = 3

Query:  576 PLFQILSLSDGERITSIIPVS 638
            PL QILS+S+GER+TSI+PVS
Sbjct:  680 PLVQILSMSEGERVTSIVPVS 700

[Close]

BLAST of HO067986 vs. SwissProt
Match: GYRA_ORYSJ (Probable DNA gyrase subunit A, chloroplastic/mitochondrial OS=Oryza sativa subsp. japonica GN=GYRA PE=2 SV=2)

HSP 1 Score: 261.536 bits (667), Expect = 4.106e-74
Identity = 130/195 (66.67%), Postives = 165/195 (84.62%), Query Frame = 1

Query:    1 ARFQLSKALQRRHVVEGILVGFNNLDRVIRIIREASSNTIAAAGLRNEFNLSEKQAEALLDMSLRRLTLRESDNFVAENKSLVEQISKLEELLSSRKNILELIEQEAIDLKNKFASPRRSILEDTDNGQLDDIDVIPNEEMLLALSEKGYLKRMKPSTFNLQNRGTIGKSVGKLKMNDSMSDFIVCRAHDYVLYF 585
            ARF+LS+AL+R+H+VEGI++G +NLD VI+IIR  S++ +A   L  EF LS+KQAEALLD++LR+LT  E   FV E KSL E+ISKL ELLS++K I +LI QEA DLKNKFA+PRRS +ED+ + ++DD+D+IPNEEMLL LSEKGY+KRMKP+TFNLQNRGTIGKSVGK++MND+ SDFIVC+ HD+VLYF
Sbjct:  444 ARFKLSQALERKHIVEGIVIGLDNLDSVIQIIRGTSNHAMARESLIKEFGLSDKQAEALLDITLRKLTSLERKKFVDEAKSLSEEISKLNELLSNKKLIFQLILQEATDLKNKFATPRRSFIEDSASTEVDDLDIIPNEEMLLILSEKGYVKRMKPNTFNLQNRGTIGKSVGKMRMNDNTSDFIVCQTHDHVLYF 638

HSP 2 Score: 38.1206 bits (87), Expect = 4.106e-74
Identity = 17/21 (80.95%), Postives = 20/21 (95.24%), Query Frame = 3

Query:  576 PLFQILSLSDGERITSIIPVS 638
            PL Q+LSLSDGERITSI+PV+
Sbjct:  663 PLVQLLSLSDGERITSIVPVN 683

[Close]

BLAST of HO067986 vs. SwissProt
Match: GYRA_SYNY3 (DNA gyrase subunit A OS=Synechocystis sp. (strain PCC 6803) GN=gyrA PE=3 SV=1)

HSP 1 Score: 161.77 bits (408), Expect = 2.817e-39
Identity = 83/194 (42.78%), Postives = 128/194 (65.98%), Query Frame = 1

Query:    4 RFQLSKALQRRHVVEGILVGFNNLDRVIRIIREASSNTIAAAGLRNEFNLSEKQAEALLDMSLRRLTLRESDNFVAENKSLVEQISKLEELLSSRKNILELIEQEAIDLKNKFASPRRSILEDTDNGQLDDIDVIPNEEMLLALSEKGYLKRMKPSTFNLQNRGTIGKSVGKLKMNDSMSDFIVCRAHDYVLYF 585
            R++L KA +R H+++G+L+  +NLD VIR+IR A+    A   L   F+LSE QA+A+L M LRRLT  E+D   AE+  L  +I+  +++L+ R+ +  +IE+E   +K   A+PRR+++   D G+L D D+I N++ L+ L+E+GY+KRM  STF  QNR T GK+  K+K +D +  F+ C  HD VL+F
Sbjct:  371 RYELRKAEERDHLLQGLLIALDNLDAVIRLIRGAADTASAKTELVEGFSLSEVQADAILQMQLRRLTALEADKITAEHDELQTKIADFQDILARRERVNAIIEEELEQIKAIHATPRRTVIVQED-GELIDTDLIANDQALILLTEQGYIKRMPASTFGTQNRATRGKAAAKIKDDDGVEHFLSCCDHDKVLFF 563

[Close]

BLAST of HO067986 vs. SwissProt
Match: GYRA_STAES (DNA gyrase subunit A OS=Staphylococcus epidermidis (strain ATCC 12228) GN=gyrA PE=3 SV=1)

HSP 1 Score: 143.665 bits (361), Expect = 7.940e-34
Identity = 72/193 (37.31%), Postives = 117/193 (60.62%), Query Frame = 1

Query:    7 FQLSKALQRRHVVEGILVGFNNLDRVIRIIREASSNTIAAAGLRNEFNLSEKQAEALLDMSLRRLTLRESDNFVAENKSLVEQISKLEELLSSRKNILELIEQEAIDLKNKFASPRRSILEDTDNGQLDDIDVIPNEEMLLALSEKGYLKRMKPSTFNLQNRGTIGKSVGKLKMNDSMSDFIVCRAHDYVLYF 585
            + L KA  R H++EG+ +  +++D +I  IRE+ ++ IA A L+  F L+E+QA+A+LDM LRRLT  E D   +E   L+E I +LEE+L+  + +L+L+  E  ++K +F   RR+ ++      L+D D+IP E++++ LS   Y+KR+  ST+  QNRG  G         D +S  +    HD+VL+F
Sbjct:  369 YNLKKARDRAHILEGLRIALDHIDEIITTIRESDTDKIAMASLQERFKLTERQAQAILDMRLRRLTGLERDKIESEYNELLEYIKELEEILADEEVLLQLVRDELTEIKERFGDERRTEIQLGGLEDLEDEDLIPEEQIVITLSHNNYIKRLPVSTYRSQNRGGRGIQGMNTLDEDFVSQLVTMSTHDHVLFF 561

[Close]

BLAST of HO067986 vs. SwissProt
Match: GYRA_STAEQ (DNA gyrase subunit A OS=Staphylococcus epidermidis (strain ATCC 35984 / RP62A) GN=gyrA PE=3 SV=1)

HSP 1 Score: 143.665 bits (361), Expect = 7.940e-34
Identity = 72/193 (37.31%), Postives = 117/193 (60.62%), Query Frame = 1

Query:    7 FQLSKALQRRHVVEGILVGFNNLDRVIRIIREASSNTIAAAGLRNEFNLSEKQAEALLDMSLRRLTLRESDNFVAENKSLVEQISKLEELLSSRKNILELIEQEAIDLKNKFASPRRSILEDTDNGQLDDIDVIPNEEMLLALSEKGYLKRMKPSTFNLQNRGTIGKSVGKLKMNDSMSDFIVCRAHDYVLYF 585
            + L KA  R H++EG+ +  +++D +I  IRE+ ++ IA A L+  F L+E+QA+A+LDM LRRLT  E D   +E   L+E I +LEE+L+  + +L+L+  E  ++K +F   RR+ ++      L+D D+IP E++++ LS   Y+KR+  ST+  QNRG  G         D +S  +    HD+VL+F
Sbjct:  369 YNLKKARDRAHILEGLRIALDHIDEIITTIRESDTDKIAMASLQERFKLTERQAQAILDMRLRRLTGLERDKIESEYNELLEYIKELEEILADEEVLLQLVRDELTEIKERFGDERRTEIQLGGLEDLEDEDLIPEEQIVITLSHNNYIKRLPVSTYRSQNRGGRGIQGMNTLDEDFVSQLVTMSTHDHVLFF 561

[Close]

BLAST of HO067986 vs. SwissProt
Match: GYRA_STAAW (DNA gyrase subunit A OS=Staphylococcus aureus (strain MW2) GN=gyrA PE=3 SV=1)

HSP 1 Score: 142.51 bits (358), Expect = 1.769e-33
Identity = 71/194 (36.60%), Postives = 117/194 (60.31%), Query Frame = 1

Query:    4 RFQLSKALQRRHVVEGILVGFNNLDRVIRIIREASSNTIAAAGLRNEFNLSEKQAEALLDMSLRRLTLRESDNFVAENKSLVEQISKLEELLSSRKNILELIEQEAIDLKNKFASPRRSILEDTDNGQLDDIDVIPNEEMLLALSEKGYLKRMKPSTFNLQNRGTIGKSVGKLKMNDSMSDFIVCRAHDYVLYF 585
            ++ L KA  R H++EG+ +  +++D +I  IRE+ ++ +A   L+  F LSEKQA+A+LDM LRRLT  E D   AE   L+  IS+LE +L+  + +L+L+  E  +++++F   RR+ ++      L+D D+IP E++++ LS   Y+KR+  ST+  QNRG  G         D +S  +    HD+VL+F
Sbjct:  368 QYNLRKAKDRAHILEGLRIALDHIDEIISTIRESDTDKVAMESLQQRFKLSEKQAQAILDMRLRRLTGLERDKIEAEYNELLNYISELEAILADEEVLLQLVRDELTEIRDRFGDDRRTEIQLGGFEDLEDEDLIPEEQIVITLSHNNYIKRLPVSTYRAQNRGGRGVQGMNTLEEDFVSQLVTLSTHDHVLFF 561

[Close]

BLAST of HO067986 vs. SwissProt
Match: GYRA_STAAU (DNA gyrase subunit A OS=Staphylococcus aureus GN=gyrA PE=1 SV=3)

HSP 1 Score: 142.51 bits (358), Expect = 1.769e-33
Identity = 71/194 (36.60%), Postives = 117/194 (60.31%), Query Frame = 1

Query:    4 RFQLSKALQRRHVVEGILVGFNNLDRVIRIIREASSNTIAAAGLRNEFNLSEKQAEALLDMSLRRLTLRESDNFVAENKSLVEQISKLEELLSSRKNILELIEQEAIDLKNKFASPRRSILEDTDNGQLDDIDVIPNEEMLLALSEKGYLKRMKPSTFNLQNRGTIGKSVGKLKMNDSMSDFIVCRAHDYVLYF 585
            ++ L KA  R H++EG+ +  +++D +I  IRE+ ++ +A   L+  F LSEKQA+A+LDM LRRLT  E D   AE   L+  IS+LE +L+  + +L+L+  E  +++++F   RR+ ++      L+D D+IP E++++ LS   Y+KR+  ST+  QNRG  G         D +S  +    HD+VL+F
Sbjct:  368 QYNLRKAKDRAHILEGLRIALDHIDEIISTIRESDTDKVAMESLQQRFKLSEKQAQAILDMRLRRLTGLERDKIEAEYNELLNYISELEAILADEEVLLQLVRDELTEIRDRFGDDRRTEIQLGGFEDLEDEDLIPEEQIVITLSHNNYIKRLPVSTYRAQNRGGRGVQGMNTLEEDFVSQLVTLSTHDHVLFF 561

[Close]

BLAST of HO067986 vs. SwissProt
Match: GYRA_STAAS (DNA gyrase subunit A OS=Staphylococcus aureus (strain MSSA476) GN=gyrA PE=3 SV=1)

HSP 1 Score: 142.51 bits (358), Expect = 1.769e-33
Identity = 71/194 (36.60%), Postives = 117/194 (60.31%), Query Frame = 1

Query:    4 RFQLSKALQRRHVVEGILVGFNNLDRVIRIIREASSNTIAAAGLRNEFNLSEKQAEALLDMSLRRLTLRESDNFVAENKSLVEQISKLEELLSSRKNILELIEQEAIDLKNKFASPRRSILEDTDNGQLDDIDVIPNEEMLLALSEKGYLKRMKPSTFNLQNRGTIGKSVGKLKMNDSMSDFIVCRAHDYVLYF 585
            ++ L KA  R H++EG+ +  +++D +I  IRE+ ++ +A   L+  F LSEKQA+A+LDM LRRLT  E D   AE   L+  IS+LE +L+  + +L+L+  E  +++++F   RR+ ++      L+D D+IP E++++ LS   Y+KR+  ST+  QNRG  G         D +S  +    HD+VL+F
Sbjct:  368 QYNLRKAKDRAHILEGLRIALDHIDEIISTIRESDTDKVAMESLQQRFKLSEKQAQAILDMRLRRLTGLERDKIEAEYNELLNYISELEAILADEEVLLQLVRDELTEIRDRFGDDRRTEIQLGGFEDLEDEDLIPEEQIVITLSHNNYIKRLPVSTYRAQNRGGRGVQGMNTLEEDFVSQLVTLSTHDHVLFF 561

[Close]

BLAST of HO067986 vs. SwissProt
Match: GYRA_STAAR (DNA gyrase subunit A OS=Staphylococcus aureus (strain MRSA252) GN=gyrA PE=3 SV=1)

HSP 1 Score: 142.51 bits (358), Expect = 1.769e-33
Identity = 71/194 (36.60%), Postives = 117/194 (60.31%), Query Frame = 1

Query:    4 RFQLSKALQRRHVVEGILVGFNNLDRVIRIIREASSNTIAAAGLRNEFNLSEKQAEALLDMSLRRLTLRESDNFVAENKSLVEQISKLEELLSSRKNILELIEQEAIDLKNKFASPRRSILEDTDNGQLDDIDVIPNEEMLLALSEKGYLKRMKPSTFNLQNRGTIGKSVGKLKMNDSMSDFIVCRAHDYVLYF 585
            ++ L KA  R H++EG+ +  +++D +I  IRE+ ++ +A   L+  F LSEKQA+A+LDM LRRLT  E D   AE   L+  IS+LE +L+  + +L+L+  E  +++++F   RR+ ++      L+D D+IP E++++ LS   Y+KR+  ST+  QNRG  G         D +S  +    HD+VL+F
Sbjct:  368 QYNLRKAKDRAHILEGLRIALDHIDEIISTIRESETDKVAMESLQQRFKLSEKQAQAILDMRLRRLTGLERDKIEAEYNELLNYISELEAILADEEVLLQLVRDELTEIRDRFGDDRRTEIQLGGFEDLEDEDLIPEEQIVITLSHNNYIKRLPVSTYRAQNRGGRGVQGMNTLEEDFVSQLVTLSTHDHVLFF 561

[Close]

BLAST of HO067986 vs. TrEMBL
Match: D7TF98_VITVI (Whole genome shotgun sequence of line PN40024, scaffold_47.assembly12x (Fragment) OS=Vitis vinifera GN=VIT_00027314001 PE=4 SV=1)

HSP 1 Score: 303.908 bits (777), Expect = 9.240e-86
Identity = 148/195 (75.90%), Postives = 181/195 (92.82%), Query Frame = 1

Query:    1 ARFQLSKALQRRHVVEGILVGFNNLDRVIRIIREASSNTIAAAGLRNEFNLSEKQAEALLDMSLRRLTLRESDNFVAENKSLVEQISKLEELLSSRKNILELIEQEAIDLKNKFASPRRSILEDTDNGQLDDIDVIPNEEMLLALSEKGYLKRMKPSTFNLQNRGTIGKSVGKLKMNDSMSDFIVCRAHDYVLYF 585
            ARF+LS+A +RRH+VEGI+VG +NLD VIR+I+EA SN +A+ GLRNEF LSE+QAEA+LD+SLRR+T  E + FV E+KSL+EQISKL+ELLSSRK IL+LIEQEAI+LKN+F++PRRS+LEDTD+GQL+D+DVIPNEEMLLA+SEKGY+KRMKP+TFNLQNRGTIGKSVGKL++ND+MSDFIVC AHDYVLYF
Sbjct:  338 ARFKLSQAQERRHIVEGIVVGLDNLDAVIRVIKEAPSNAMASTGLRNEFGLSERQAEAILDISLRRITRLEREKFVTESKSLMEQISKLQELLSSRKQILQLIEQEAIELKNRFSTPRRSMLEDTDSGQLEDVDVIPNEEMLLAVSEKGYVKRMKPNTFNLQNRGTIGKSVGKLRVNDAMSDFIVCHAHDYVLYF 532

HSP 2 Score: 38.5058 bits (88), Expect = 9.240e-86
Identity = 19/21 (90.48%), Postives = 19/21 (90.48%), Query Frame = 3

Query:  576 PLFQILSLSDGERITSIIPVS 638
            PL QIL LSDGERITSIIPVS
Sbjct:  557 PLVQILCLSDGERITSIIPVS 577

[Close]

BLAST of HO067986 vs. TrEMBL
Match: B9HVG1_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_805680 PE=4 SV=1)

HSP 1 Score: 296.975 bits (759), Expect = 6.487e-84
Identity = 148/195 (75.90%), Postives = 179/195 (91.79%), Query Frame = 1

Query:    1 ARFQLSKALQRRHVVEGILVGFNNLDRVIRIIREASSNTIAAAGLRNEFNLSEKQAEALLDMSLRRLTLRESDNFVAENKSLVEQISKLEELLSSRKNILELIEQEAIDLKNKFASPRRSILEDTDNGQLDDIDVIPNEEMLLALSEKGYLKRMKPSTFNLQNRGTIGKSVGKLKMNDSMSDFIVCRAHDYVLYF 585
            A F+LS+A +RRH+VEG++ G +NLDRV+ IIR+ASSN IA+A LRNEF+LSEKQAEA+LD+SLRRLTL E   FV E+KSL+EQI+KLEELLSSR NIL+LIEQEA++LKNKF++PRRS+LED+D+GQL+DIDVIPNEEMLLA+SEKGY+KRMKP+TFNLQNRGTIGKSVGKL+ +D+MSDFIVC AHD VLYF
Sbjct:  443 AMFKLSEAQKRRHIVEGVMAGLDNLDRVVDIIRKASSNAIASADLRNEFSLSEKQAEAILDISLRRLTLLEGKKFVEESKSLMEQITKLEELLSSRGNILQLIEQEAVELKNKFSNPRRSMLEDSDSGQLEDIDVIPNEEMLLAISEKGYVKRMKPNTFNLQNRGTIGKSVGKLRDSDAMSDFIVCHAHDRVLYF 637

HSP 2 Score: 39.2762 bits (90), Expect = 6.487e-84
Identity = 19/21 (90.48%), Postives = 19/21 (90.48%), Query Frame = 3

Query:  576 PLFQILSLSDGERITSIIPVS 638
            PL Q LSLSDGERITSIIPVS
Sbjct:  662 PLIQFLSLSDGERITSIIPVS 682

[Close]

BLAST of HO067986 vs. TrEMBL
Match: D7L923_ARALY (DNA gyrase subunit A family protein OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_478403 PE=4 SV=1)

HSP 1 Score: 261.536 bits (667), Expect = 1.008e-72
Identity = 127/195 (65.13%), Postives = 171/195 (87.69%), Query Frame = 1

Query:    1 ARFQLSKALQRRHVVEGILVGFNNLDRVIRIIREASSNTIAAAGLRNEFNLSEKQAEALLDMSLRRLTLRESDNFVAENKSLVEQISKLEELLSSRKNILELIEQEAIDLKNKFASPRRSILEDTDNGQLDDIDVIPNEEMLLALSEKGYLKRMKPSTFNLQNRGTIGKSVGKLKMNDSMSDFIVCRAHDYVLYF 585
            ARF+LS A QR+H +EGI+VG +N+D VI++I+ ASS++ A+A L++E+ LSEKQAEA+L+++LRRLT  E      E+ SL EQI+KLE+LLS+R NIL+LIEQEAI+LK++F+SPRRS+LED+D+G L+DIDVIPNEEML+A+SEKGY+K+MK  TFNLQ+RGTIGKSVGKL+++D+MSDF+VC AHD+VL+F
Sbjct:  461 ARFKLSHAQQRKHNIEGIVVGLDNVDEVIKLIKNASSHSSASAALQSEYGLSEKQAEAILEITLRRLTTLERKKVTDESSSLTEQITKLEQLLSTRANILKLIEQEAIELKDRFSSPRRSMLEDSDSGDLEDIDVIPNEEMLMAISEKGYVKKMKADTFNLQHRGTIGKSVGKLRVDDAMSDFLVCHAHDHVLFF 655

HSP 2 Score: 37.3502 bits (85), Expect = 1.008e-72
Identity = 16/21 (76.19%), Postives = 20/21 (95.24%), Query Frame = 3

Query:  576 PLFQILSLSDGERITSIIPVS 638
            PL QILS+S+GER+TSI+PVS
Sbjct:  680 PLVQILSMSEGERVTSIVPVS 700

[Close]

BLAST of HO067986 vs. TrEMBL
Match: C5WVZ2_SORBI (Putative uncharacterized protein Sb01g003750 OS=Sorghum bicolor GN=Sb01g003750 PE=4 SV=1)

HSP 1 Score: 251.906 bits (642), Expect = 5.913e-70
Identity = 126/196 (64.29%), Postives = 166/196 (84.69%), Query Frame = 1

Query:    1 ARFQLSKALQRRHVVEGILVGFNNLDRVIRIIREASSNTIAAAGLRNEFNLSEKQAEALLDMSLRRLTLRESDNFVAENKSLVEQISKLEELLSSRKNILELIEQEAIDLKNKFASPRRSILEDTDNGQLDDIDVIPNEEMLL-ALSEKGYLKRMKPSTFNLQNRGTIGKSVGKLKMNDSMSDFIVCRAHDYVLYF 585
            AR++LS+AL+R+H+VEGI++G +NLD VI++IRE S++T A   L  EF+LSEKQAEALLD++L+RLT  E   FV E K L E+ISKL ELLSS++ I +LI+QEA DLKNKF++PRRS+++D+ + ++DDID+IPNEEMLL  LSEKGY KRM P+TF+LQ+RGTIGKSVGK+++NDS SDFIVC+ HD+VLYF
Sbjct:  456 ARYKLSQALERKHIVEGIVIGLDNLDAVIQMIRETSNHTAATGALVKEFDLSEKQAEALLDITLKRLTSLERKKFVDEAKILSEEISKLNELLSSKELIFQLIQQEAADLKNKFSTPRRSLIDDSVSCEVDDIDIIPNEEMLLKILSEKGYAKRMNPNTFSLQHRGTIGKSVGKMRLNDSTSDFIVCQTHDHVLYF 651

HSP 2 Score: 37.7354 bits (86), Expect = 5.913e-70
Identity = 18/21 (85.71%), Postives = 19/21 (90.48%), Query Frame = 3

Query:  576 PLFQILSLSDGERITSIIPVS 638
            PL Q+L LSDGERITSIIPVS
Sbjct:  676 PLVQLLPLSDGERITSIIPVS 696

[Close]

BLAST of HO067986 vs. TrEMBL
Match: B9F6X5_ORYSJ (Putative uncharacterized protein OS=Oryza sativa subsp. japonica GN=OsJ_13077 PE=4 SV=1)

HSP 1 Score: 246.899 bits (629), Expect = 1.430e-68
Identity = 129/223 (57.85%), Postives = 166/223 (74.44%), Query Frame = 1

Query:    1 ARFQLSKALQRRHVVEGILVGFNNLDRVIRIIREASSNTIAAAGLRNEFNLSEKQAEALLDMSLRRLTLRESDNFVAENKSLVEQISKLEELLSSRK----------------------------NILELIEQEAIDLKNKFASPRRSILEDTDNGQLDDIDVIPNEEMLLALSEKGYLKRMKPSTFNLQNRGTIGKSVGKLKMNDSMSDFIVCRAHDYVLYF 585
            ARF+LS+AL+R+H+VEGI++G +NLD VI+IIR  S++ +A   L  EF LS+KQAEALLD++LR+LT  E   FV E KSL E+ISKL ELLS++K                            ++ +LI QEA DLKNKFA+PRRS +ED+ + ++DD+D+IPNEEMLL LSEKGY+KRMKP+TFNLQNRGTIGKSVGK++MND+ SDFIVC+ HD+VLYF
Sbjct:  349 ARFKLSQALERKHIVEGIVIGLDNLDSVIQIIRGTSNHAMARESLIKEFGLSDKQAEALLDITLRKLTSLERKKFVDEAKSLSEEISKLNELLSNKKLIFQGLVDGLDYLRAEINVVIRELKLMWSLFKLILQEATDLKNKFATPRRSFIEDSASTEVDDLDIIPNEEMLLILSEKGYVKRMKPNTFNLQNRGTIGKSVGKMRMNDNTSDFIVCQTHDHVLYF 571

HSP 2 Score: 38.1206 bits (87), Expect = 1.430e-68
Identity = 17/21 (80.95%), Postives = 20/21 (95.24%), Query Frame = 3

Query:  576 PLFQILSLSDGERITSIIPVS 638
            PL Q+LSLSDGERITSI+PV+
Sbjct:  596 PLVQLLSLSDGERITSIVPVN 616

[Close]

BLAST of HO067986 vs. TrEMBL
Match: B8AM88_ORYSI (Putative uncharacterized protein OS=Oryza sativa subsp. indica GN=OsI_14033 PE=4 SV=1)

HSP 1 Score: 246.899 bits (629), Expect = 1.430e-68
Identity = 129/223 (57.85%), Postives = 166/223 (74.44%), Query Frame = 1

Query:    1 ARFQLSKALQRRHVVEGILVGFNNLDRVIRIIREASSNTIAAAGLRNEFNLSEKQAEALLDMSLRRLTLRESDNFVAENKSLVEQISKLEELLSSRK----------------------------NILELIEQEAIDLKNKFASPRRSILEDTDNGQLDDIDVIPNEEMLLALSEKGYLKRMKPSTFNLQNRGTIGKSVGKLKMNDSMSDFIVCRAHDYVLYF 585
            ARF+LS+AL+R+H+VEGI++G +NLD VI+IIR  S++ +A   L  EF LS+KQAEALLD++LR+LT  E   FV E KSL E+ISKL ELLS++K                            ++ +LI QEA DLKNKFA+PRRS +ED+ + ++DD+D+IPNEEMLL LSEKGY+KRMKP+TFNLQNRGTIGKSVGK++MND+ SDFIVC+ HD+VLYF
Sbjct:  349 ARFKLSQALERKHIVEGIVIGLDNLDSVIQIIRGTSNHAMARESLIKEFGLSDKQAEALLDITLRKLTSLERKKFVDEAKSLSEEISKLNELLSNKKLIFQGLVDGLDYLRAEINVVIRELKLMWSLFKLILQEATDLKNKFATPRRSFIEDSASTEVDDLDIIPNEEMLLILSEKGYVKRMKPNTFNLQNRGTIGKSVGKMRMNDNTSDFIVCQTHDHVLYF 571

HSP 2 Score: 38.1206 bits (87), Expect = 1.430e-68
Identity = 17/21 (80.95%), Postives = 20/21 (95.24%), Query Frame = 3

Query:  576 PLFQILSLSDGERITSIIPVS 638
            PL Q+LSLSDGERITSI+PV+
Sbjct:  596 PLVQLLSLSDGERITSIVPVN 616

[Close]

BLAST of HO067986 vs. TrEMBL
Match: B9SK05_RICCO (DNA gyrase subunit A, chloroplast/mitochondrial, putative OS=Ricinus communis GN=RCOM_0720220 PE=4 SV=1)

HSP 1 Score: 201.83 bits (512), Expect = 8.916e-56
Identity = 100/127 (78.74%), Postives = 114/127 (89.76%), Query Frame = 1

Query:  205 LRESDNFVAENKSLVEQISKLEELLSSRKNILELIEQEAIDLKNKFASPRRSILEDTDNGQLDDIDVIPNEEMLLALSEKGYLKRMKPSTFNLQNRGTIGKSVGKLKMNDSMSDFIVCRAHDYVLYF 585
            L +   FV E+K L+EQIS+LEELLSSR NIL+LIEQEA++LKNKFASPRRS LED+D  QLDDIDV+PNEEMLLA SEKGY+KRMKP TFNLQNRGTIGKSVGKL++ND+MSD IVCRAHD+VLYF
Sbjct:    2 LLQRKKFVNESKLLMEQISRLEELLSSRHNILQLIEQEAVELKNKFASPRRSSLEDSDGSQLDDIDVVPNEEMLLAFSEKGYVKRMKPDTFNLQNRGTIGKSVGKLRVNDAMSDSIVCRAHDHVLYF 128

HSP 2 Score: 40.4318 bits (93), Expect = 8.916e-56
Identity = 20/21 (95.24%), Postives = 20/21 (95.24%), Query Frame = 3

Query:  576 PLFQILSLSDGERITSIIPVS 638
            PL QILSLSDGERITSIIPVS
Sbjct:  153 PLVQILSLSDGERITSIIPVS 173

[Close]

BLAST of HO067986 vs. TrEMBL
Match: A9SV22_PHYPA (Predicted protein (Fragment) OS=Physcomitrella patens subsp. patens GN=PHYPADRAFT_135715 PE=4 SV=1)

HSP 1 Score: 209.92 bits (533), Expect = 1.367e-53
Identity = 102/195 (52.31%), Postives = 143/195 (73.33%), Query Frame = 1

Query:    1 ARFQLSKALQRRHVVEGILVGFNNLDRVIRIIREASSNTIAAAGLRNEFNLSEKQAEALLDMSLRRLTLRESDNFVAENKSLVEQISKLEELLSSRKNILELIEQEAIDLKNKFASPRRSILEDTDNGQLDDIDVIPNEEMLLALSEKGYLKRMKPSTFNLQNRGTIGKSVGKLKMNDSMSDFIVCRAHDYVLYF 585
            A+FQL +A  R H+VEGIL+G +NLD V++IIR A     A+ GL+  FN++  QA+ALL M LRRLT  ES     E+ +L  +I+ L++LL S+  +L++IE+EA+ LK ++ +PRR+++E   +G+++DIDVI NEE ++ LSEKGY+KRM   TF  QNRGT GKS GK++ ND+MSDF VCR HDYVL+F
Sbjct:  390 AKFQLKRAEDRDHIVEGILIGLSNLDGVVKIIRSAKDTAQASEGLQKGFNVTPAQADALLAMPLRRLTSLESGKLKEEHDALTAEITDLKQLLESKHRVLQIIEKEALVLKKEYGTPRRTLVEQGRDGEINDIDVIANEETIVTLSEKGYIKRMAAGTFTAQNRGTTGKSGGKMRSNDAMSDFFVCRTHDYVLFF 584

HSP 2 Score: 25.0238 bits (53), Expect = 1.367e-53
Identity = 11/21 (52.38%), Postives = 15/21 (71.43%), Query Frame = 3

Query:  576 PLFQILSLSDGERITSIIPVS 638
            PL Q++ +  GERITS+  VS
Sbjct:  609 PLVQVVPVPPGERITSVQSVS 629

[Close]

BLAST of HO067986 vs. TrEMBL
Match: Q0DME7_ORYSJ (Os03g0812000 protein (Fragment) OS=Oryza sativa subsp. japonica GN=Os03g0812000 PE=4 SV=1)

HSP 1 Score: 195.282 bits (495), Expect = 3.971e-53
Identity = 96/137 (70.07%), Postives = 118/137 (86.13%), Query Frame = 1

Query:  175 LLDMSLRRLTLRESDNFVAENKSLVEQISKLEELLSSRKNILELIEQEAIDLKNKFASPRRSILEDTDNGQLDDIDVIPNEEMLLALSEKGYLKRMKPSTFNLQNRGTIGKSVGKLKMNDSMSDFIVCRAHDYVLYF 585
            LLD++LR+LT  E   FV E KSL E+ISKL ELLS++K I +LI QEA DLKNKFA+PRRS +ED+ + ++DD+D+IPNEEMLL LSEKGY+KRMKP+TFNLQNRGTIGKSVGK++MND+ SDFIVC+ HD+VLYF
Sbjct:    1 LLDITLRKLTSLERKKFVDEAKSLSEEISKLNELLSNKKLIFQLILQEATDLKNKFATPRRSFIEDSASTEVDDLDIIPNEEMLLILSEKGYVKRMKPNTFNLQNRGTIGKSVGKMRMNDNTSDFIVCQTHDHVLYF 137

HSP 2 Score: 38.1206 bits (87), Expect = 3.971e-53
Identity = 17/21 (80.95%), Postives = 20/21 (95.24%), Query Frame = 3

Query:  576 PLFQILSLSDGERITSIIPVS 638
            PL Q+LSLSDGERITSI+PV+
Sbjct:  162 PLVQLLSLSDGERITSIVPVN 182

[Close]

BLAST of HO067986 vs. TrEMBL
Match: Q2HTL3_MEDTR (WD40-like OS=Medicago truncatula GN=MtrDRAFT_AC150269g2v2 PE=4 SV=1)

HSP 1 Score: 182.185 bits (461), Expect = 6.650e-50
Identity = 90/104 (86.54%), Postives = 98/104 (94.23%), Query Frame = 1

Query:  274 LLSSRKNILELIEQEAIDLKNKFASPRRSILEDTDNGQLDDIDVIPNEEMLLALSEKGYLKRMKPSTFNLQNRGTIGKSVGKLKMNDSMSDFIVCRAHDYVLYF 585
            L S   + +++IEQEAIDLKNKFASPRRSILEDTD+GQL+DIDVIPNEEMLL LSEKGYLKRMKPSTFNLQ+RGTIGKSVGKLKMNDSMSDFIVCRAHD+VLYF
Sbjct:    5 LKSQLDSSIQVIEQEAIDLKNKFASPRRSILEDTDDGQLEDIDVIPNEEMLLTLSEKGYLKRMKPSTFNLQHRGTIGKSVGKLKMNDSMSDFIVCRAHDFVLYF 108

HSP 2 Score: 40.4318 bits (93), Expect = 6.650e-50
Identity = 20/21 (95.24%), Postives = 20/21 (95.24%), Query Frame = 3

Query:  576 PLFQILSLSDGERITSIIPVS 638
            PL QILSLSDGERITSIIPVS
Sbjct:  133 PLVQILSLSDGERITSIIPVS 153

[Close]

BLAST of HO067986 vs. TAIR peptide
Match: AT3G10690.1 (| Symbols: GYRA | DNA GYRASE A | chr3:3339612-3346243 REVERSE LENGTH=950)

HSP 1 Score: 265.388 bits (677), Expect = 5.030e-76
Identity = 129/195 (66.15%), Postives = 171/195 (87.69%), Query Frame = 1

Query:    1 ARFQLSKALQRRHVVEGILVGFNNLDRVIRIIREASSNTIAAAGLRNEFNLSEKQAEALLDMSLRRLTLRESDNFVAENKSLVEQISKLEELLSSRKNILELIEQEAIDLKNKFASPRRSILEDTDNGQLDDIDVIPNEEMLLALSEKGYLKRMKPSTFNLQNRGTIGKSVGKLKMNDSMSDFIVCRAHDYVLYF 585
            ARF+LS A QR+H++EGI+VG +N+D VI +I +ASS++ A A L++E+ LSEKQAEA+L+++LRRLT  E   F  E+ SL EQI+KLE+LLS+R NIL+LIEQEAI+LK++F+SPRRS+LED+D+G L+DIDVIPNEEML+A+SEKGY+KRMK  TFNLQ+RGTIGKSVGKL+++D+MSDF+VC AHD+VL+F
Sbjct:  461 ARFKLSHAQQRKHIIEGIVVGLDNVDEVIELITKASSHSSATAALQSEYGLSEKQAEAILEITLRRLTALERKKFTDESSSLTEQITKLEQLLSTRTNILKLIEQEAIELKDRFSSPRRSMLEDSDSGDLEDIDVIPNEEMLMAVSEKGYVKRMKADTFNLQHRGTIGKSVGKLRVDDAMSDFLVCHAHDHVLFF 655

HSP 2 Score: 37.3502 bits (85), Expect = 5.030e-76
Identity = 16/21 (76.19%), Postives = 20/21 (95.24%), Query Frame = 3

Query:  576 PLFQILSLSDGERITSIIPVS 638
            PL QILS+S+GER+TSI+PVS
Sbjct:  680 PLVQILSMSEGERVTSIVPVS 700

The following BLAST results are available for this feature:

BLAST of HO067986 vs. SwissProt
BLAST of HO067986 vs. TrEMBL
BLAST of HO067986 vs. TAIR peptide

BLAST of HO067986 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Swissprot)
Total hits: 10


Match Name	E-value	Identity	Description
GYRA_NICBE	1.422e-83	74.87	DNA gyrase subunit A, chloroplastic/mitochondrial ...	[more]
GYRA_ARATH	4.905e-75	66.15	Probable DNA gyrase subunit A, chloroplastic/mitoc...	[more]
GYRA_ORYSJ	4.106e-74	66.67	Probable DNA gyrase subunit A, chloroplastic/mitoc...	[more]
GYRA_SYNY3	2.817e-39	42.78	DNA gyrase subunit A OS=Synechocystis sp. (strain ...	[more]
GYRA_STAES	7.940e-34	37.31	DNA gyrase subunit A OS=Staphylococcus epidermidis...	[more]
GYRA_STAEQ	7.940e-34	37.31	DNA gyrase subunit A OS=Staphylococcus epidermidis...	[more]
GYRA_STAAW	1.769e-33	36.60	DNA gyrase subunit A OS=Staphylococcus aureus (str...	[more]
GYRA_STAAU	1.769e-33	36.60	DNA gyrase subunit A OS=Staphylococcus aureus GN=g...	[more]
GYRA_STAAS	1.769e-33	36.60	DNA gyrase subunit A OS=Staphylococcus aureus (str...	[more]
GYRA_STAAR	1.769e-33	36.60	DNA gyrase subunit A OS=Staphylococcus aureus (str...	[more]

BLAST of HO067986 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
D7TF98_VITVI	9.240e-86	75.90	Whole genome shotgun sequence of line PN40024, sca...	[more]
B9HVG1_POPTR	6.487e-84	75.90	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
D7L923_ARALY	1.008e-72	65.13	DNA gyrase subunit A family protein OS=Arabidopsis...	[more]
C5WVZ2_SORBI	5.913e-70	64.29	Putative uncharacterized protein Sb01g003750 OS=So...	[more]
B9F6X5_ORYSJ	1.430e-68	57.85	Putative uncharacterized protein OS=Oryza sativa s...	[more]
B8AM88_ORYSI	1.430e-68	57.85	Putative uncharacterized protein OS=Oryza sativa s...	[more]
B9SK05_RICCO	8.916e-56	78.74	DNA gyrase subunit A, chloroplast/mitochondrial, p...	[more]
A9SV22_PHYPA	1.367e-53	52.31	Predicted protein (Fragment) OS=Physcomitrella pat...	[more]
Q0DME7_ORYSJ	3.971e-53	70.07	Os03g0812000 protein (Fragment) OS=Oryza sativa su...	[more]
Q2HTL3_MEDTR	6.650e-50	86.54	WD40-like OS=Medicago truncatula GN=MtrDRAFT_AC150...	[more]

BLAST of HO067986 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide)
Total hits: 1


Match Name	E-value	Identity	Description
AT3G10690.1	5.030e-76	66.15	\| Symbols: GYRA \| DNA GYRASE A \| chr3:3339612-3346...	[more]

InterPro

Analysis Name: InterProScan analysis for Cicer arietinum unigene v1
Date Performed: 2010-12-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002205	DNA topoisomerase, type IIA, subunit A/C-terminal	PFAM	PF00521	DNA_topoisoIV	coord: 2..99 score: 3.7
IPR006691	DNA gyrase/topoisomerase IV, subunit A, C-terminal beta-pinwheel	PFAM	PF03989	DNA_gyraseA_C	coord: 140..188 score: 4.6
IPR013757	DNA topoisomerase, type IIA, subunit A, alpha-helical	GENE3D	1.10.268.10	no description	coord: 2..95 score: 6.2
IPR013760	DNA topoisomerase, type IIA, central	SUPERFAMILY	56719	Type II DNA topoisomerase	coord: 2..126 score: 1.5
None	No IPR available	SUPERFAMILY	101904	GyrA/ParC C-terminal domain-like	coord: 135..195 score: 9.4
None	No IPR available	SEG	seg	seg	coord: 17..41 score: NAcoord: 55..70 score

Properties


Property Name	Value
Singlet in analysis	Cicer arietinum unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>HO067986 ID=HO067986; Name=HO067986; organism=Cicer arietinum; type=EST; length=640bp
GCAAGATTTCAGTTATCAAAAGCACTACAGAGAAGGCATGTTGTTGAGGG
TATTCTTGTTGGGTTCAATAATCTGGATAGGGTGATTCGCATAATACGAG
AAGCTTCAAGTAACACAATTGCTGCAGCTGGGCTACGGAATGAGTTCAAT
CTCTCTGAGAAACAAGCTGAAGCTCTTTTGGACATGAGCCTAAGAAGACT
GACCCTTCGTGAGAGTGACAACTTTGTGGCTGAAAATAAATCTTTGGTCG
AACAAATTTCCAAGTTAGAGGAACTGTTGTCCAGCAGGAAAAATATACTG
GAGCTTATAGAACAAGAAGCAATTGATCTGAAAAATAAATTTGCTAGTCC
AAGGCGATCAATTTTGGAGGACACAGATAATGGTCAACTTGACGACATTG
ATGTTATTCCAAATGAAGAAATGCTATTGGCACTTAGTGAAAAAGGTTAT
TTGAAGCGTATGAAGCCAAGCACTTTTAACCTTCAAAATCGTGGAACAAT
TGGCAAATCAGTAGGCAAACTGAAAATGAATGATTCTATGTCTGATTTCA
TCGTTTGTCGTGCACATGATTATGTCCTTTATTTCAGATCTTATCTTTAT
CTGATGGTGAAAGAATAACATCCATTATTCCTGTGAGTGA

Annotated Terms

The following terms have been associated with this EST:

Vocabulary: **Molecular Function**
Term	Definition
GO:0003677	DNA binding
GO:0003918	DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) activity
GO:0005524	ATP binding
GO:0003916	DNA topoisomerase activity

Vocabulary: **Cellular Component**
Term	Definition
GO:0005694	chromosome

Vocabulary: **Biological Process**
Term	Definition
GO:0006265	DNA topological change
GO:0006259	DNA metabolic process

Vocabulary: **INTERPRO**
Term	Definition
IPR002205	Topo_IIA_dom_A
IPR006691	GyrA/parC_rep
IPR013757	Topo_IIA_A_a_sf
IPR013760	Topo_IIA-like_dom_sf

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