FE672413

EST Overview


Name	FE672413
Unique Name	FE672413
Type	EST
Organism	Cicer arietinum (chickpea)
Sequence length	610

Libraries


Library Name	Type
LIBEST_022820 Drought-stressed chickpea leave library	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Cicer arietinum unigene v1	Cicer arietinum unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Cicer arietinum unigene v1	2010-12-27
Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide	2011-02-03
Homology Analysis: Cicer arietinum unigene v1 vs Swissprot	2010-12-28
Homology Analysis: Cicer arietinum unigene v1 vs Trembl	2010-12-28
InterProScan analysis for Cicer arietinum unigene v1	2010-12-29

Homology

[Close]

BLAST of FE672413 vs. SwissProt
Match: RPAB1_CAEEL (DNA-directed RNA polymerases I, II, and III subunit RPABC1 OS=Caenorhabditis elegans GN=rpb-5 PE=2 SV=1)

HSP 1 Score: 91.2781 bits (225), Expect = 4.213e-18
Identity = 50/141 (35.46%), Postives = 82/141 (58.16%), Query Frame = 1

Query:  202 MVFSEEELTKLFRVYKTVVQMINDRNYVVSDSDLTMSKQDFKSKFGEHVK-----REDLVIKAPNRDKSSDQIYVFFCDDAKXXXXXXXXXXNRMTTDNVGRGIIVCQSQLTGPARNALGEISSSKHRIEIFLEDELLVNV 609
            M   E E  +L+R+ KTV+QM++DR Y+V+  +L    + FK ++G+        R DL I   + D  +DQ++VFF +DAK           +M   N+ R IIV Q+ +T  A+ ++G++ + K+ +E FLE EL+VN+
Sbjct:    1 MADDELETYRLWRIRKTVLQMVHDRGYLVAQDELDQPLETFKVQYGDRPSEKKPARSDLTILVAHNDDPADQMFVFFPEDAKIGIKTIKAICQQMQEQNISRAIIVVQTGMTPSAKQSIGDM-APKYMLEHFLEAELMVNI 140

[Close]

BLAST of FE672413 vs. SwissProt
Match: RPAB1_CAEBR (DNA-directed RNA polymerases I, II, and III subunit RPABC1 OS=Caenorhabditis briggsae GN=rpb-5 PE=3 SV=1)

HSP 1 Score: 90.8929 bits (224), Expect = 5.503e-18
Identity = 50/141 (35.46%), Postives = 80/141 (56.74%), Query Frame = 1

Query:  202 MVFSEEELTKLFRVYKTVVQMINDRNYVVSDSDLTMSKQDFKSKFGEHVK-----REDLVIKAPNRDKSSDQIYVFFCDDAKXXXXXXXXXXNRMTTDNVGRGIIVCQSQLTGPARNALGEISSSKHRIEIFLEDELLVNV 609
            M   E E  +L+R+ KTV+QM++DR Y+V+  +L    + FK  FG+        R DL I   + D  +DQ++VFF +D K           +M   N+ R IIV Q+ +T  A+ ++G++ + K+ +E FLE EL+VN+
Sbjct:    1 MADDELETYRLWRIRKTVLQMVHDRGYLVAQEELDQPLETFKETFGDRPSEKKPARSDLTILVAHNDDPADQMFVFFPEDTKIGIKTIKAICQQMQEQNISRAIIVVQTGMTPSAKQSIGDM-APKYMLEHFLEAELMVNI 140

[Close]

BLAST of FE672413 vs. SwissProt
Match: RPAB1_RAT (DNA-directed RNA polymerases I, II, and III subunit RPABC1 OS=Rattus norvegicus GN=Polr2e PE=2 SV=1)

HSP 1 Score: 87.0409 bits (214), Expect = 7.946e-17
Identity = 44/137 (32.12%), Postives = 83/137 (60.58%), Query Frame = 1

Query:  214 EEELTKLFRVYKTVVQMINDRNYVVSDSDLTMSKQDFKSKFGE-----HVKREDLVIKAPNRDKSSDQIYVFFCDDAKXXXXXXXXXXNRMTTDNVGRGIIVCQSQLTGPARNALGEISSSKHRIEIFLEDELLVNV 609
            EEE  +L+++ KT++Q+ +DR Y+V+  +L  + ++FK++FG+       +R DL +   + D  +DQ++VFF ++ K           RM  +N+ R +IV Q  +T  A+ +L ++ + K+ +E FL+ ELL+N+
Sbjct:    4 EEETYRLWKIRKTIMQLCHDRGYLVTQDELDQTLEEFKAQFGDKPSEGRPRRTDLTVLVAHNDDPTDQMFVFFPEEPKVGIKTIKVYCQRMQEENITRALIVVQQGMTPSAKQSLVDM-APKYVLEQFLQQELLINI 139

[Close]

BLAST of FE672413 vs. SwissProt
Match: RPAB1_MOUSE (DNA-directed RNA polymerases I, II, and III subunit RPABC1 OS=Mus musculus GN=Polr2e PE=2 SV=1)

HSP 1 Score: 87.0409 bits (214), Expect = 7.946e-17
Identity = 44/137 (32.12%), Postives = 83/137 (60.58%), Query Frame = 1

Query:  214 EEELTKLFRVYKTVVQMINDRNYVVSDSDLTMSKQDFKSKFGE-----HVKREDLVIKAPNRDKSSDQIYVFFCDDAKXXXXXXXXXXNRMTTDNVGRGIIVCQSQLTGPARNALGEISSSKHRIEIFLEDELLVNV 609
            EEE  +L+++ KT++Q+ +DR Y+V+  +L  + ++FK++FG+       +R DL +   + D  +DQ++VFF ++ K           RM  +N+ R +IV Q  +T  A+ +L ++ + K+ +E FL+ ELL+N+
Sbjct:    4 EEETYRLWKIRKTIMQLCHDRGYLVTQDELDQTLEEFKAQFGDKPSEGRPRRTDLTVLVAHNDDPTDQMFVFFPEEPKVGIKTIKVYCQRMQEENITRALIVVQQGMTPSAKQSLVDM-APKYVLEQFLQQELLINI 139

[Close]

BLAST of FE672413 vs. SwissProt
Match: RPAB1_PONAB (DNA-directed RNA polymerases I, II, and III subunit RPABC1 OS=Pongo abelii GN=POLR2E PE=2 SV=1)

HSP 1 Score: 85.8853 bits (211), Expect = 1.770e-16
Identity = 43/137 (31.39%), Postives = 83/137 (60.58%), Query Frame = 1

Query:  214 EEELTKLFRVYKTVVQMINDRNYVVSDSDLTMSKQDFKSKFGE-----HVKREDLVIKAPNRDKSSDQIYVFFCDDAKXXXXXXXXXXNRMTTDNVGRGIIVCQSQLTGPARNALGEISSSKHRIEIFLEDELLVNV 609
            EEE  +L+++ KT++Q+ +DR Y+V+  +L  + ++F+++FG+       +R DL +   + D  +DQ++VFF ++ K           RM  +N+ R +IV Q  +T  A+ +L ++ + K+ +E FL+ ELL+N+
Sbjct:    4 EEETYRLWKIRKTIMQLCHDRGYLVTQDELDQTLEEFRAQFGDKPSEGRPRRTDLTVLVAHNDDPTDQMFVFFPEEPKVGIKTIKVYCQRMQEENITRALIVVQQGMTPSAKQSLVDM-APKYILEQFLQQELLINI 139

[Close]

BLAST of FE672413 vs. SwissProt
Match: RPAB1_BOVIN (DNA-directed RNA polymerases I, II, and III subunit RPABC1 OS=Bos taurus GN=POLR2E PE=2 SV=1)

HSP 1 Score: 84.3445 bits (207), Expect = 5.150e-16
Identity = 44/137 (32.12%), Postives = 81/137 (59.12%), Query Frame = 1

Query:  214 EEELTKLFRVYKTVVQMINDRNYVVSDSDLTMSKQDFKSKFGE-----HVKREDLVIKAPNRDKSSDQIYVFFCDDAKXXXXXXXXXXNRMTTDNVGRGIIVCQSQLTGPARNALGEISSSKHRIEIFLEDELLVNV 609
            EEE  +L+++ KT++Q+ +DR Y+V+   L  + ++FK++FG        +R DL +   + D  +DQ++VFF ++ K           RM  +N+ R +IV Q  +T  A+ +L ++ + K+ +E FL+ ELL+N+
Sbjct:    4 EEETYRLWKIRKTIMQLCHDRGYLVTQDGLDQTLEEFKAQFGGKPSEGRPRRTDLTVLVAHNDDPTDQMFVFFPEEPKVGIKTIKVYCQRMQEENITRALIVVQQGMTPSAKQSLVDM-APKYILEQFLQQELLINI 139

[Close]

BLAST of FE672413 vs. SwissProt
Match: RPAB1_HUMAN (DNA-directed RNA polymerases I, II, and III subunit RPABC1 OS=Homo sapiens GN=POLR2E PE=1 SV=4)

HSP 1 Score: 83.9593 bits (206), Expect = 6.727e-16
Identity = 43/137 (31.39%), Postives = 82/137 (59.85%), Query Frame = 1

Query:  214 EEELTKLFRVYKTVVQMINDRNYVVSDSDLTMSKQDFKSKFGE-----HVKREDLVIKAPNRDKSSDQIYVFFCDDAKXXXXXXXXXXNRMTTDNVGRGIIVCQSQLTGPARNALGEISSSKHRIEIFLEDELLVNV 609
            EEE  +L+++ KT++Q+ +DR Y+V+  +L  + ++FK++ G+       +R DL +   + D  +DQ++VFF ++ K           RM  +N+ R +IV Q  +T  A+ +L ++ + K+ +E FL+ ELL+N+
Sbjct:    4 EEETYRLWKIRKTIMQLCHDRGYLVTQDELDQTLEEFKAQSGDKPSEGRPRRTDLTVLVAHNDDPTDQMFVFFPEEPKVGIKTIKVYCQRMQEENITRALIVVQQGMTPSAKQSLVDM-APKYILEQFLQQELLINI 139

[Close]

BLAST of FE672413 vs. SwissProt
Match: RPAB1_DICDI (DNA-directed RNA polymerases I, II, and III subunit rpabc1 OS=Dictyostelium discoideum GN=polr2e PE=3 SV=2)

HSP 1 Score: 73.1738 bits (178), Expect = 1.187e-12
Identity = 45/132 (34.09%), Postives = 68/132 (51.52%), Query Frame = 1

Query:  217 EELTKLFRVYKTVVQMINDRNYVVSDSDLTMSKQDFKSKFGEHVKREDLVIKAPNRDKSSDQIYVFFCDDAKXXXXXXXXXXNRMTTDNVGRGIIVCQSQLTGPARNALGEISSSKH-RIEIFLEDELLVNV 609
            E++ +L ++ KTV+QM++DR Y+++  +    ++ F       V RE+L +    +D  +D IYVFF D  K          + M      R IIV Q  +T  A+ AL E S  K   +E F E ELLVN+
Sbjct:    2 EDINRLLQIRKTVLQMLSDREYIITAYEQEYPRESF-------VSRENLTMYCVKKDDPTDSIYVFFPDTPKVGVTQIKKYVDIMKDRQANRAIIVVQQNITPFAKQALAEFSQEKKITLEQFNEAELLVNI 126

[Close]

BLAST of FE672413 vs. SwissProt
Match: COX2_DAUCA (Cytochrome c oxidase subunit 2 OS=Daucus carota GN=COX2 PE=3 SV=1)

HSP 1 Score: 41.2022 bits (95), Expect = 4.214e-9
Identity = 21/25 (84.00%), Postives = 21/25 (84.00%), Query Frame = -2

Query:   61 SGQ*SDAVPGGLNQISISVQREGVY 135
            SG   DAVPG LNQISISVQREGVY
Sbjct:  195 SGVKCDAVPGRLNQISISVQREGVY 219

HSP 2 Score: 40.0466 bits (92), Expect = 4.214e-9
Identity = 18/20 (90.00%), Postives = 18/20 (90.00%), Query Frame = -3

Query:    9 GFTSEICGTNHAFTPIVVEA 68
            G  SEICGTNHAFTPIVVEA
Sbjct:  221 GQCSEICGTNHAFTPIVVEA 240

[Close]

BLAST of FE672413 vs. SwissProt
Match: RPAB1_KLULA (DNA-directed RNA polymerases I, II, and III subunit RPABC1 OS=Kluyveromyces lactis GN=RPB5 PE=3 SV=1)

HSP 1 Score: 60.8474 bits (146), Expect = 6.098e-9
Identity = 38/141 (26.95%), Postives = 75/141 (53.19%), Query Frame = 1

Query:  214 EEELTKLFRVYKTVVQMINDRNYVVSDSDLTMSKQDFKSKFGEHV---KREDLVIKAPNRDKSSDQ------IYVFFCDDAKXXXXXXXXXXNRMTTDNVGRGIIVCQSQLTGPARNALGEISSSKHRIEIFLEDELLVNV 609
            E  +++L+R +KTV +M+ DR Y ++  ++ +S +DFK+K+ + +   +R+ +  ++   ++S ++      ++V FCD+A             +T  N   GI + QS +T  A   L   +++   IE F E  L+VN+
Sbjct:    6 ERNISRLWRAFKTVKEMVRDRGYFITQEEIDLSLEDFKAKYCDSMGKPQRKMMSFQSNPTEESIEKFPEMGSLWVEFCDEASVGVKTMKNFVVHITEKNFQTGIFIYQSGITPSANKILP--TAAPAVIETFPEASLVVNI 144

[Close]

BLAST of FE672413 vs. TrEMBL
Match: C6SXZ4_SOYBN (Putative uncharacterized protein OS=Glycine max PE=2 SV=1)

HSP 1 Score: 141.354 bits (355), Expect = 5.086e-32
Identity = 72/136 (52.94%), Postives = 95/136 (69.85%), Query Frame = 1

Query:  202 MVFSEEELTKLFRVYKTVVQMINDRNYVVSDSDLTMSKQDFKSKFGEHVKREDLVIKAPNRDKSSDQIYVFFCDDAKXXXXXXXXXXNRMTTDNVGRGIIVCQSQLTGPARNALGEISSSKHRIEIFLEDELLVNV 609
            MV SEEE+ +L+R+ KTV+QM+ DRN++V D ++ MSK +FKSK+GEH+KREDLVI    +D S DQIYVFF D+ K          NRM ++NV R I+V Q+ LT  AR  + EISS  H +E+F E ELLVN+
Sbjct:    1 MVLSEEEIGRLYRIRKTVMQMLRDRNFLVGDFEINMSKHEFKSKYGEHMKREDLVINKSKKDNSGDQIYVFFPDEPKVGVKTMKTYTNRMNSENVYRAILVTQTNLTPFARTCISEISSKFH-LEVFQEAELLVNI 135

[Close]

BLAST of FE672413 vs. TrEMBL
Match: C6SVA4_SOYBN (Putative uncharacterized protein OS=Glycine max PE=2 SV=1)

HSP 1 Score: 139.043 bits (349), Expect = 2.524e-31
Identity = 71/136 (52.21%), Postives = 94/136 (69.12%), Query Frame = 1

Query:  202 MVFSEEELTKLFRVYKTVVQMINDRNYVVSDSDLTMSKQDFKSKFGEHVKREDLVIKAPNRDKSSDQIYVFFCDDAKXXXXXXXXXXNRMTTDNVGRGIIVCQSQLTGPARNALGEISSSKHRIEIFLEDELLVNV 609
            MV SEEE+ +L+R+ KTV+QM+ DR ++V D ++ MSK +FKSK+GEH+KREDLVI    +D S DQIYVFF D+ K          NRM ++NV R I+V Q+ LT  AR  + EISS  H +E+F E ELLVN+
Sbjct:    1 MVLSEEEMGRLYRIRKTVMQMLRDRGFLVGDFEINMSKHEFKSKYGEHMKREDLVINKSKKDNSGDQIYVFFPDEPKVGVKTMKTYTNRMNSENVYRAILVTQTNLTPFARTCISEISSKFH-LEVFQEAELLVNI 135

[Close]

BLAST of FE672413 vs. TrEMBL
Match: O81098_ARATH (AT3g22320/MCB17_5 OS=Arabidopsis thaliana GN=AtRPABC24.3 PE=1 SV=1)

HSP 1 Score: 126.331 bits (316), Expect = 1.693e-27
Identity = 64/135 (47.41%), Postives = 92/135 (68.15%), Query Frame = 1

Query:  205 VFSEEELTKLFRVYKTVVQMINDRNYVVSDSDLTMSKQDFKSKFGEHVKREDLVIKAPNRDKSSDQIYVFFCDDAKXXXXXXXXXXNRMTTDNVGRGIIVCQSQLTGPARNALGEISSSKHRIEIFLEDELLVNV 609
            + +EEEL +L+R+ KT++QM+ DR Y ++DS+LTM+KQ F  K G+++KREDLV     R+ +SDQ+Y+FF D+AK          NRM ++NV R I+V Q  LT  AR  + EISS  H +E+F E E+LVN+
Sbjct:    1 MLTEEELKRLYRIQKTLMQMLRDRGYFIADSELTMTKQQFIRKHGDNMKREDLVTLKAKRNDNSDQLYIFFPDEAKVGVKTMKMYTNRMKSENVFRAILVVQQNLTPFARTCISEISSKFH-LEVFQEAEMLVNI 134

[Close]

BLAST of FE672413 vs. TrEMBL
Match: B9N7D1_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_828412 PE=4 SV=1)

HSP 1 Score: 126.331 bits (316), Expect = 1.693e-27
Identity = 65/136 (47.79%), Postives = 91/136 (66.91%), Query Frame = 1

Query:  202 MVFSEEELTKLFRVYKTVVQMINDRNYVVSDSDLTMSKQDFKSKFGEHVKREDLVIKAPNRDKSSDQIYVFFCDDAKXXXXXXXXXXNRMTTDNVGRGIIVCQSQLTGPARNALGEISSSKHRIEIFLEDELLVNV 609
            M  +EEE+ +L R+ KTV+QM+ DR Y V D ++ M+++ F+SK+G ++KREDLVI    R+ SSDQIYVFF ++AK          NRM ++NV R I+V Q  LT  AR  + EIS+  H +E+F E ELLVN+
Sbjct:    1 MTLTEEEIKRLLRIRKTVMQMLKDRGYFVGDFEIKMTREQFESKYGNNMKREDLVINKTKRNDSSDQIYVFFPEEAKVGVKTMKTYTNRMKSENVFRAILVVQQNLTPFARTCINEISTKFH-LEVFQEAELLVNI 135

[Close]

BLAST of FE672413 vs. TrEMBL
Match: D7L1N3_ARALY (ATRPABC24.3 OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_479745 PE=4 SV=1)

HSP 1 Score: 125.176 bits (313), Expect = 3.772e-27
Identity = 63/135 (46.67%), Postives = 92/135 (68.15%), Query Frame = 1

Query:  205 VFSEEELTKLFRVYKTVVQMINDRNYVVSDSDLTMSKQDFKSKFGEHVKREDLVIKAPNRDKSSDQIYVFFCDDAKXXXXXXXXXXNRMTTDNVGRGIIVCQSQLTGPARNALGEISSSKHRIEIFLEDELLVNV 609
            + +EEE+ +L+R+ KT++QM+ DR Y ++DS+LTM+KQ F  K G+++KREDLV     R+ +SDQ+Y+FF D+AK          NRM ++NV R I+V Q  LT  AR  + EISS  H +E+F E E+LVN+
Sbjct:    1 MLTEEEIKRLYRIQKTLMQMLKDRGYFIADSELTMTKQQFIRKHGDNMKREDLVTLKVKRNDNSDQLYIFFPDEAKVGVKTMKMYTNRMKSENVYRAILVVQQNLTPFARTCISEISSKFH-LEVFQEAEMLVNI 134

[Close]

BLAST of FE672413 vs. TrEMBL
Match: B3TLL2_ELAGV (DNA-directed RNA polymerases II 24 kDa polypeptide OS=Elaeis guineensis var. tenera PE=2 SV=1)

HSP 1 Score: 122.094 bits (305), Expect = 3.193e-26
Identity = 63/133 (47.37%), Postives = 89/133 (66.92%), Query Frame = 1

Query:  211 SEEELTKLFRVYKTVVQMINDRNYVVSDSDLTMSKQDFKSKFGEHVKREDLVIKAPNRDKSSDQIYVFFCDDAKXXXXXXXXXXNRMTTDNVGRGIIVCQSQLTGPARNALGEISSSKHRIEIFLEDELLVNV 609
            SE+++++LFR+ +TV+QM+ DR Y+V D ++ M+K  F  KFGE VKREDLVI    ++  +DQIYVFF +DAK           RM  +NV RGI+V Q  LT  AR +L E+ S K+ +E+F + ELLVN+
Sbjct:    2 SEDDISRLFRIRRTVMQMLRDRGYLVLDLEINMNKLQFLEKFGEGVKREDLVINKAKKNDPADQIYVFFPNDAKVGVKQIKQYVERMKAENVTRGILVVQQALTPFARQSLAEV-SQKYMLEVFQDTELLVNI 133

[Close]

BLAST of FE672413 vs. TrEMBL
Match: A9RWG5_PHYPA (Predicted protein OS=Physcomitrella patens subsp. patens GN=PHYPADRAFT_206246 PE=4 SV=1)

HSP 1 Score: 120.939 bits (302), Expect = 7.113e-26
Identity = 59/132 (44.70%), Postives = 92/132 (69.70%), Query Frame = 1

Query:  214 EEELTKLFRVYKTVVQMINDRNYVVSDSDLTMSKQDFKSKFGEHVKREDLVIKAPNRDKSSDQIYVFFCDDAKXXXXXXXXXXNRMTTDNVGRGIIVCQSQLTGPARNALGEISSSKHRIEIFLEDELLVNV 609
            +E+  +L+R+ KTV++M+ DR+YVV+D +LT+SK+ F+ K+G+  KREDLVI+ P R  +++ I+VFF ++AK          +RM T+NV R I+V Q  LT  AR  + E+ +SK+ +E+F E ELLVN+
Sbjct:    7 DEQSARLYRIRKTVMEMLRDRDYVVADYELTLSKEQFREKYGDEPKREDLVIQKPRRSNNAEHIFVFFPEEAKVGVKTIKTYVDRMKTENVHRAILVVQQNLTPFARQCVSEM-ASKYHLEVFQEAELLVNI 137

[Close]

BLAST of FE672413 vs. TrEMBL
Match: A9RKG6_PHYPA (Predicted protein OS=Physcomitrella patens subsp. patens GN=PHYPADRAFT_55574 PE=4 SV=1)

HSP 1 Score: 117.857 bits (294), Expect = 6.022e-25
Identity = 57/132 (43.18%), Postives = 91/132 (68.94%), Query Frame = 1

Query:  214 EEELTKLFRVYKTVVQMINDRNYVVSDSDLTMSKQDFKSKFGEHVKREDLVIKAPNRDKSSDQIYVFFCDDAKXXXXXXXXXXNRMTTDNVGRGIIVCQSQLTGPARNALGEISSSKHRIEIFLEDELLVNV 609
            +E+  +L+R+ KTV++M+ DR+YVV++ +L  +K++F+ K+G+  KREDLVI+ P R  +++ I+VFF ++AK          +RM T+NV R I+V Q  LT  AR  + E+ SSK+ +E+F E ELLVN+
Sbjct:    7 DEQCARLYRIRKTVMEMLRDRDYVVAEFELNSTKEEFREKYGDEPKREDLVIQKPKRSNNAEHIFVFFPEEAKVGVKTIKTYVDRMKTENVHRAILVVQQNLTPFARQCVSEM-SSKYHVEVFQEAELLVNI 137

[Close]

BLAST of FE672413 vs. TrEMBL
Match: B9RKM1_RICCO (DNA-directed RNA polymerase II, putative OS=Ricinus communis GN=RCOM_1051580 PE=4 SV=1)

HSP 1 Score: 114.775 bits (286), Expect = 5.098e-24
Identity = 64/155 (41.29%), Postives = 91/155 (58.71%), Query Frame = 1

Query:  202 MVFSEEELTKLFRVYKTVVQMINDRNYVVSDSDLTMSKQDFKSKFGEHVKREDLVIKAPNRDKSSD-------------------QIYVFFCDDAKXXXXXXXXXXNRMTTDNVGRGIIVCQSQLTGPARNALGEISSSKHRIEIFLEDELLVNV 609
            M  SE+E+ +LFR+ +TV+QM+ DR Y+V+DS++ ++K  F SK+G+++KREDLVI    RD S                     QIYVFF ++ K          NRM ++NV R I+V Q  LT  AR  + EI+S  H +E+F E ELLVN+
Sbjct:    1 MTLSEDEIKRLFRIRRTVMQMLKDRTYLVTDSEINITKHQFTSKYGDNMKREDLVIHKSKRDGSDSFYPTKLLFLNQLEAWLPLYQIYVFFPEEQKVGVKTMKTYTNRMKSENVFRAILVVQQSLTPFARTCIAEIASKFH-LEVFQESELLVNI 154

[Close]

BLAST of FE672413 vs. TrEMBL
Match: A9NTD1_PICSI (Putative uncharacterized protein OS=Picea sitchensis PE=2 SV=1)

HSP 1 Score: 110.923 bits (276), Expect = 7.361e-23
Identity = 58/132 (43.94%), Postives = 84/132 (63.64%), Query Frame = 1

Query:  214 EEELTKLFRVYKTVVQMINDRNYVVSDSDLTMSKQDFKSKFGEHVKREDLVIKAPNRDKSSDQIYVFFCDDAKXXXXXXXXXXNRMTTDNVGRGIIVCQSQLTGPARNALGEISSSKHRIEIFLEDELLVNV 609
            +E+  KLFR+ +TV+QM+NDR+Y+V D ++  +K+ F  KFGE  K+EDLVI    R+  S+QI+VFF ++AK           RM ++ V R I+V Q  LT  AR ++ E S   H +E+F E ELLVN+
Sbjct:    4 DEQTNKLFRIRRTVLQMLNDRDYLVGDFEINTTKEQFLDKFGEDPKKEDLVINKAKRNSPSEQIFVFFPEEAKVGVKSIKTYVERMKSEGVFRAILVVQQALTPFARQSVQEFSGKFH-LEVFQEAELLVNI 134

[Close]

BLAST of FE672413 vs. TAIR peptide
Match: AT3G22320.1 (| Symbols: ATRPABC24.3, RPB5A, NRPB5, NRPD5 | Eukaryotic rpb5 RNA polymerase subunit family protein | chr3:7891045-7892094 REVERSE LENGTH=205)

HSP 1 Score: 126.331 bits (316), Expect = 1.247e-29
Identity = 64/135 (47.41%), Postives = 92/135 (68.15%), Query Frame = 1

Query:  205 VFSEEELTKLFRVYKTVVQMINDRNYVVSDSDLTMSKQDFKSKFGEHVKREDLVIKAPNRDKSSDQIYVFFCDDAKXXXXXXXXXXNRMTTDNVGRGIIVCQSQLTGPARNALGEISSSKHRIEIFLEDELLVNV 609
            + +EEEL +L+R+ KT++QM+ DR Y ++DS+LTM+KQ F  K G+++KREDLV     R+ +SDQ+Y+FF D+AK          NRM ++NV R I+V Q  LT  AR  + EISS  H +E+F E E+LVN+
Sbjct:    1 MLTEEELKRLYRIQKTLMQMLRDRGYFIADSELTMTKQQFIRKHGDNMKREDLVTLKAKRNDNSDQLYIFFPDEAKVGVKTMKMYTNRMKSENVFRAILVVQQNLTPFARTCISEISSKFH-LEVFQEAEMLVNI 134

[Close]

BLAST of FE672413 vs. TAIR peptide
Match: AT5G57980.1 (| Symbols: RPB5C | RNA polymerase II fifth largest subunit, C | chr5:23469050-23469768 REVERSE LENGTH=210)

HSP 1 Score: 82.8037 bits (203), Expect = 1.581e-16
Identity = 46/136 (33.82%), Postives = 78/136 (57.35%), Query Frame = 1

Query:  214 EEELTKLFRVYKTVVQMINDRNYVVSDSDLTMSKQDFKSKFGE---HVKREDLVIKAPNRDKSSDQIYVFFCDDAKXXXXXXXXXXN-RMTTDNVGRGIIVCQSQLTGPARNALGEISSSKHRIEIFLEDELLVNV 609
            ++E+T++F+V +TV+QM+ DR Y + +SDL + +++F  +F +    V +E L + A      +D+IYVF+ +  K            +M  D V RGI+V    +T PAR A+ E++     IE+F E EL+ N+
Sbjct:    5 DDEITRIFKVRRTVLQMLRDRGYTIEESDLNLKREEFVQRFCKTMNKVNKEALFVSANKGPNPADKIYVFYPEGPKVGVPVIKKEVAIKMRDDKVHRGIVVVPMAITAPARMAVSELNKML-TIEVFEEAELVTNI 139

[Close]

BLAST of FE672413 vs. TAIR peptide
Match: AT2G41340.1 (| Symbols: RPB5D | RNA polymerase II fifth largest subunit, D | chr2:17229026-17230442 FORWARD LENGTH=218)

HSP 1 Score: 65.0846 bits (157), Expect = 3.411e-11
Identity = 46/134 (34.33%), Postives = 71/134 (52.99%), Query Frame = 1

Query:  211 SEEELTKLFRVYKTVVQMINDRNYVVSDSDLTMSKQDFKSKFGEHVKREDLVIKAPNRDKSSDQIYVFFCDDA-KXXXXXXXXXXNRMTTDNVGRGIIVCQSQLTGPARNALGEISSSKHRIEIFLEDELLVNV 609
            S  E  K +   +T ++M+ DR Y VSD D+ +S Q F++ +GEH   + L I A +R  SS +I V FC               + ++ +N+   I+V QS +T  A  A+ E+ S K  +E+F   +LLVNV
Sbjct:   17 SSVECHKYYLARRTTMEMLRDRGYDVSDEDINLSLQQFRALYGEHPDVDLLRISAKHRFDSSKKISVVFCGTGIVKVNAMRVIAADVLSRENITGLILVLQSHITNQALKAV-ELFSFK--VELFEITDLLVNV 147

[Close]

BLAST of FE672413 vs. TAIR peptide
Match: AT3G57080.1 (| Symbols: RPB5B, NRPE5 | Eukaryotic rpb5 RNA polymerase subunit family protein | chr3:21125878-21127470 FORWARD LENGTH=222)

HSP 1 Score: 57.3806 bits (137), Expect = 7.113e-9
Identity = 41/134 (30.60%), Postives = 69/134 (51.49%), Query Frame = 1

Query:  211 SEEELTKLFRVYKTVVQMINDRNYVVSDSDLTMSKQDFKSKFGEHVKREDLVIKAPNRDKSSDQI-YVFFCDDAKXXXXXXXXXXNRMTTDNVGRGIIVCQSQLTGPARNALGEISSSKHRIEIFLEDELLVNV 609
            S EE  + +   +  +QM+ DR Y VSD D+ +S  DF++ +GE    + L I A +R  S+ ++  VFF               + ++ + +   I+V Q+ +T  A  A+ E+ S K  +EIF   +LLVN+
Sbjct:   21 SSEESHRYYLARRNGLQMLRDRGYEVSDEDINLSLHDFRTVYGERPDVDRLRISALHRSDSTKKVKIVFFGTSMVKVNAIRSVVADILSQETITGLILVLQNHVTNQALKAI-ELFSFK--VEIFQITDLLVNI 151

[Close]

BLAST of FE672413 vs. TAIR peptide
Match: ATMG00160.1 (| Symbols: COX2 | cytochrome oxidase 2 | chrM:40502-42628 REVERSE LENGTH=260)

HSP 1 Score: 38.891 bits (89), Expect = 1.672e-8
Identity = 20/25 (80.00%), Postives = 20/25 (80.00%), Query Frame = -2

Query:   61 SGQ*SDAVPGGLNQISISVQREGVY 135
            SG   DAVPG LNQISI VQREGVY
Sbjct:  194 SGVKCDAVPGRLNQISILVQREGVY 218

HSP 2 Score: 36.965 bits (84), Expect = 1.672e-8
Identity = 17/20 (85.00%), Postives = 17/20 (85.00%), Query Frame = -3

Query:    9 GFTSEICGTNHAFTPIVVEA 68
            G  SEICGTNHAFT IVVEA
Sbjct:  220 GQCSEICGTNHAFTSIVVEA 239

The following BLAST results are available for this feature:

BLAST of FE672413 vs. SwissProt
BLAST of FE672413 vs. TrEMBL
BLAST of FE672413 vs. TAIR peptide

BLAST of FE672413 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Swissprot)
Total hits: 10


Match Name	E-value	Identity	Description
RPAB1_CAEEL	4.213e-18	35.46	DNA-directed RNA polymerases I, II, and III subuni...	[more]
RPAB1_CAEBR	5.503e-18	35.46	DNA-directed RNA polymerases I, II, and III subuni...	[more]
RPAB1_RAT	7.946e-17	32.12	DNA-directed RNA polymerases I, II, and III subuni...	[more]
RPAB1_MOUSE	7.946e-17	32.12	DNA-directed RNA polymerases I, II, and III subuni...	[more]
RPAB1_PONAB	1.770e-16	31.39	DNA-directed RNA polymerases I, II, and III subuni...	[more]
RPAB1_BOVIN	5.150e-16	32.12	DNA-directed RNA polymerases I, II, and III subuni...	[more]
RPAB1_HUMAN	6.727e-16	31.39	DNA-directed RNA polymerases I, II, and III subuni...	[more]
RPAB1_DICDI	1.187e-12	34.09	DNA-directed RNA polymerases I, II, and III subuni...	[more]
COX2_DAUCA	4.214e-9	84.00	Cytochrome c oxidase subunit 2 OS=Daucus carota GN...	[more]
RPAB1_KLULA	6.098e-9	26.95	DNA-directed RNA polymerases I, II, and III subuni...	[more]

BLAST of FE672413 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
C6SXZ4_SOYBN	5.086e-32	52.94	Putative uncharacterized protein OS=Glycine max PE...	[more]
C6SVA4_SOYBN	2.524e-31	52.21	Putative uncharacterized protein OS=Glycine max PE...	[more]
O81098_ARATH	1.693e-27	47.41	AT3g22320/MCB17_5 OS=Arabidopsis thaliana GN=AtRPA...	[more]
B9N7D1_POPTR	1.693e-27	47.79	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
D7L1N3_ARALY	3.772e-27	46.67	ATRPABC24.3 OS=Arabidopsis lyrata subsp. lyrata GN...	[more]
B3TLL2_ELAGV	3.193e-26	47.37	DNA-directed RNA polymerases II 24 kDa polypeptide...	[more]
A9RWG5_PHYPA	7.113e-26	44.70	Predicted protein OS=Physcomitrella patens subsp. ...	[more]
A9RKG6_PHYPA	6.022e-25	43.18	Predicted protein OS=Physcomitrella patens subsp. ...	[more]
B9RKM1_RICCO	5.098e-24	41.29	DNA-directed RNA polymerase II, putative OS=Ricinu...	[more]
A9NTD1_PICSI	7.361e-23	43.94	Putative uncharacterized protein OS=Picea sitchens...	[more]

BLAST of FE672413 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide)
Total hits: 5


Match Name	E-value	Identity	Description
AT3G22320.1	1.247e-29	47.41	\| Symbols: ATRPABC24.3, RPB5A, NRPB5, NRPD5 \| Euka...	[more]
AT5G57980.1	1.581e-16	33.82	\| Symbols: RPB5C \| RNA polymerase II fifth largest...	[more]
AT2G41340.1	3.411e-11	34.33	\| Symbols: RPB5D \| RNA polymerase II fifth largest...	[more]
AT3G57080.1	7.113e-9	30.60	\| Symbols: RPB5B, NRPE5 \| Eukaryotic rpb5 RNA poly...	[more]
ATMG00160.1	1.672e-8	80.00	\| Symbols: COX2 \| cytochrome oxidase 2 \| chrM:4050...	[more]

InterPro

Analysis Name: InterProScan analysis for Cicer arietinum unigene v1
Date Performed: 2010-12-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR005571	RNA polymerase, Rpb5, N-terminal	GENE3D	3.40.1340.10	no description	coord: 12..142 score: 5.3
IPR005571	RNA polymerase, Rpb5, N-terminal	PFAM	PF03871	RNA_pol_Rpb5_N	coord: 12..98 score: 7.4
IPR005571	RNA polymerase, Rpb5, N-terminal	SUPERFAMILY	53036	Eukaryotic RPB5 N-terminal domain	coord: 12..143 score: 6.2
None	No IPR available	PANTHER	PTHR10535	DNA-DIRECTED RNA POLYMERASE II	coord: 12..144 score: 6
None	No IPR available	SEG	seg	seg	coord: 86..95 score

Properties


Property Name	Value
Singlet in analysis	Cicer arietinum unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>FE672413 ID=FE672413; Name=FE672413; organism=Cicer arietinum; type=EST; length=610bp
ATCGCATAAGCTTCTACGACGATAGGCGTAAAGGCATGATTAGTTCCACA
AATCTCACTAGTAAACCCCTTCTCGTTGTACCGAAATAGATATCTGATTT
AAACCACCAGGTACAGCATCACTCTATTGTCCACTTCTAATTCTCAACAA
ACAAAAAAACACAAATAGGGTCTGTAGGGTTTAACGCTTTACGACAGAGA
CATGGTGTTTTCAGAAGAAGAACTAACCAAACTATTCAGAGTCTACAAAA
CAGTAGTACAAATGATAAACGACAGAAACTACGTCGTTTCAGATTCCGAC
CTAACCATGTCGAAACAAGATTTCAAATCCAAATTCGGAGAACACGTTAA
GAGAGAAGACCTAGTTATTAAAGCACCCAATAGAGATAAATCTTCTGATC
AAATCTATGTGTTCTTTTGTGATGACGCTAAAGTTAGTGTTAGGGTTGTT
AGAAGTTTTTTTAACCGTATGACGACTGATAATGTTGGTAGAGGGATTAT
TGTTTGTCAGAGTCAGCTTACTGGACCTGCCAGGAATGCTCTCGGTGAAA
TTTCTTCGTCCAAACATCGGATTGAAATCTTTCTGGAGGATGAACTGTTG
GTGAACGTAA

Annotated Terms

The following terms have been associated with this EST:

Vocabulary: **Molecular Function**
Term	Definition
GO:0003677	DNA binding
GO:0003899	DNA-directed 5'-3' RNA polymerase activity

Vocabulary: **Cellular Component**
Term	Definition
GO:0005634	nucleus

Vocabulary: **Biological Process**
Term	Definition
GO:0006350	transcription

Vocabulary: **INTERPRO**
Term	Definition
IPR005571	RNA_pol_Rpb5_N

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