HO064247

EST Overview


Name	HO064247
Unique Name	HO064247
Type	EST
Organism	Cicer arietinum (chickpea)
Sequence length	639

Libraries


Library Name	Type
LIBEST_026411 Chickpea drought stressed cDNA SSH library AR2	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Cicer arietinum unigene v1	Cicer arietinum unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Cicer arietinum unigene v1	2010-12-27
Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide	2011-02-03
Homology Analysis: Cicer arietinum unigene v1 vs Swissprot	2010-12-28
Homology Analysis: Cicer arietinum unigene v1 vs Trembl	2010-12-28
InterProScan analysis for Cicer arietinum unigene v1	2010-12-29

Homology

[Close]

BLAST of HO064247 vs. SwissProt
Match: KINB2_ARATH (SNF1-related protein kinase regulatory subunit beta-2 OS=Arabidopsis thaliana GN=KINB2 PE=1 SV=1)

HSP 1 Score: 68.1662 bits (165), Expect = 1.053e-13
Identity = 29/42 (69.05%), Postives = 37/42 (88.10%), Query Frame = -1

Query:  487 NLEIQPPMPRPQHGVLNHLYVQKGKSSPSVVALGTTHRYMAQ 612
            N +I  P+PRPQH +LNHLY+QKGKS PSVVALG+THR++A+
Sbjct:  236 NPDIPSPLPRPQHVILNHLYMQKGKSGPSVVALGSTHRFLAK 277

HSP 2 Score: 28.8758 bits (63), Expect = 1.053e-13
Identity = 13/13 (100.00%), Postives = 13/13 (100.00%), Query Frame = -2

Query:  450 KYVTVVLYKSLQR 488
            KYVTVVLYKSLQR
Sbjct:  277 KYVTVVLYKSLQR 289

[Close]

BLAST of HO064247 vs. TrEMBL
Match: D7SSK4_VITVI (Whole genome shotgun sequence of line PN40024, scaffold_259.assembly12x (Fragment) OS=Vitis vinifera GN=VIT_00005136001 PE=4 SV=1)

HSP 1 Score: 69.3218 bits (168), Expect = 7.001e-13
Identity = 31/41 (75.61%), Postives = 37/41 (90.24%), Query Frame = -1

Query:  487 LEIQPPMPRPQHGVLNHLYVQKGKSSPSVVALGTTHRYMAQ 609
            +EI PPMPRPQH VLNHLY+QKGKS PSVVALG+T+R+ A+
Sbjct:  211 VEIPPPMPRPQHVVLNHLYMQKGKSGPSVVALGSTNRFRAK 251

HSP 2 Score: 28.8758 bits (63), Expect = 7.001e-13
Identity = 13/13 (100.00%), Postives = 13/13 (100.00%), Query Frame = -2

Query:  450 KYVTVVLYKSLQR 488
            KYVTVVLYKSLQR
Sbjct:  251 KYVTVVLYKSLQR 263

[Close]

BLAST of HO064247 vs. TrEMBL
Match: A5AP67_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_040057 PE=4 SV=1)

HSP 1 Score: 69.3218 bits (168), Expect = 7.004e-13
Identity = 31/41 (75.61%), Postives = 37/41 (90.24%), Query Frame = -1

Query:  487 LEIQPPMPRPQHGVLNHLYVQKGKSSPSVVALGTTHRYMAQ 609
            +EI PPMPRPQH VLNHLY+QKGKS PSVVALG+T+R+ A+
Sbjct:  208 VEIPPPMPRPQHVVLNHLYMQKGKSGPSVVALGSTNRFRAK 248

HSP 2 Score: 28.8758 bits (63), Expect = 7.004e-13
Identity = 13/13 (100.00%), Postives = 13/13 (100.00%), Query Frame = -2

Query:  450 KYVTVVLYKSLQR 488
            KYVTVVLYKSLQR
Sbjct:  248 KYVTVVLYKSLQR 260

[Close]

BLAST of HO064247 vs. TrEMBL
Match: D7SSK2_VITVI (Whole genome shotgun sequence of line PN40024, scaffold_259.assembly12x (Fragment) OS=Vitis vinifera GN=VIT_00005134001 PE=4 SV=1)

HSP 1 Score: 69.3218 bits (168), Expect = 7.681e-13
Identity = 31/41 (75.61%), Postives = 37/41 (90.24%), Query Frame = -1

Query:  487 LEIQPPMPRPQHGVLNHLYVQKGKSSPSVVALGTTHRYMAQ 609
            +EI PPMPRPQH VLNHLY+QKGKS PSVVALG+T+R+ A+
Sbjct:   67 VEIPPPMPRPQHVVLNHLYMQKGKSGPSVVALGSTNRFRAK 107

HSP 2 Score: 28.8758 bits (63), Expect = 7.681e-13
Identity = 13/13 (100.00%), Postives = 13/13 (100.00%), Query Frame = -2

Query:  450 KYVTVVLYKSLQR 488
            KYVTVVLYKSLQR
Sbjct:  107 KYVTVVLYKSLQR 119

[Close]

BLAST of HO064247 vs. TrEMBL
Match: Q7XYX7_MEDTR (AKIN beta2 OS=Medicago truncatula PE=2 SV=1)

HSP 1 Score: 69.707 bits (169), Expect = 9.064e-13
Identity = 31/44 (70.45%), Postives = 39/44 (88.64%), Query Frame = -1

Query:  487 TTNLEIQPPMPRPQHGVLNHLYVQKGKSSPSVVALGTTHRYMAQ 618
            + N+E QP + RPQH VLNHLY+QKGKSSPSVVALG+THR++A+
Sbjct:  232 SANMEFQPLVSRPQHVVLNHLYMQKGKSSPSVVALGSTHRFVAK 275

HSP 2 Score: 28.1054 bits (61), Expect = 9.064e-13
Identity = 12/13 (92.31%), Postives = 13/13 (100.00%), Query Frame = -2

Query:  450 KYVTVVLYKSLQR 488
            KYVTVV+YKSLQR
Sbjct:  275 KYVTVVMYKSLQR 287

[Close]

BLAST of HO064247 vs. TrEMBL
Match: B9IH18_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_906596 PE=4 SV=1)

HSP 1 Score: 68.1662 bits (165), Expect = 1.535e-12
Identity = 29/41 (70.73%), Postives = 37/41 (90.24%), Query Frame = -1

Query:  487 LEIQPPMPRPQHGVLNHLYVQKGKSSPSVVALGTTHRYMAQ 609
            +EI PP+ RPQH VLNHLY+QKGKS P+VVALG+THR++A+
Sbjct:  209 MEIPPPLSRPQHVVLNHLYMQKGKSGPAVVALGSTHRFLAK 249

HSP 2 Score: 28.8758 bits (63), Expect = 1.535e-12
Identity = 13/13 (100.00%), Postives = 13/13 (100.00%), Query Frame = -2

Query:  450 KYVTVVLYKSLQR 488
            KYVTVVLYKSLQR
Sbjct:  249 KYVTVVLYKSLQR 261

[Close]

BLAST of HO064247 vs. TrEMBL
Match: Q2V3H9_ARATH (Uncharacterized protein At4g16360.2 OS=Arabidopsis thaliana GN=At4g16360 PE=4 SV=2)

HSP 1 Score: 68.1662 bits (165), Expect = 1.536e-12
Identity = 29/42 (69.05%), Postives = 37/42 (88.10%), Query Frame = -1

Query:  487 NLEIQPPMPRPQHGVLNHLYVQKGKSSPSVVALGTTHRYMAQ 612
            N +I  P+PRPQH +LNHLY+QKGKS PSVVALG+THR++A+
Sbjct:  205 NPDIPSPLPRPQHVILNHLYMQKGKSGPSVVALGSTHRFLAK 246

HSP 2 Score: 28.8758 bits (63), Expect = 1.536e-12
Identity = 13/13 (100.00%), Postives = 13/13 (100.00%), Query Frame = -2

Query:  450 KYVTVVLYKSLQR 488
            KYVTVVLYKSLQR
Sbjct:  246 KYVTVVLYKSLQR 258

[Close]

BLAST of HO064247 vs. TrEMBL
Match: D7M9W3_ARALY (AMP-activated protein kinase OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_915121 PE=4 SV=1)

HSP 1 Score: 68.1662 bits (165), Expect = 1.536e-12
Identity = 29/42 (69.05%), Postives = 37/42 (88.10%), Query Frame = -1

Query:  487 NLEIQPPMPRPQHGVLNHLYVQKGKSSPSVVALGTTHRYMAQ 612
            N +I  P+PRPQH +LNHLY+QKGKS PSVVALG+THR++A+
Sbjct:  205 NPDIPSPLPRPQHVILNHLYMQKGKSGPSVVALGSTHRFLAK 246

HSP 2 Score: 28.8758 bits (63), Expect = 1.536e-12
Identity = 13/13 (100.00%), Postives = 13/13 (100.00%), Query Frame = -2

Query:  450 KYVTVVLYKSLQR 488
            KYVTVVLYKSLQR
Sbjct:  246 KYVTVVLYKSLQR 258

[Close]

BLAST of HO064247 vs. TrEMBL
Match: C0Z307_ARATH (AT4G16360 protein OS=Arabidopsis thaliana GN=AT4G16360 PE=4 SV=1)

HSP 1 Score: 68.1662 bits (165), Expect = 1.686e-12
Identity = 29/42 (69.05%), Postives = 37/42 (88.10%), Query Frame = -1

Query:  487 NLEIQPPMPRPQHGVLNHLYVQKGKSSPSVVALGTTHRYMAQ 612
            N +I  P+PRPQH +LNHLY+QKGKS PSVVALG+THR++A+
Sbjct:   57 NPDIPSPLPRPQHVILNHLYMQKGKSGPSVVALGSTHRFLAK 98

HSP 2 Score: 28.8758 bits (63), Expect = 1.686e-12
Identity = 13/13 (100.00%), Postives = 13/13 (100.00%), Query Frame = -2

Query:  450 KYVTVVLYKSLQR 488
            KYVTVVLYKSLQR
Sbjct:   98 KYVTVVLYKSLQR 110

[Close]

BLAST of HO064247 vs. TrEMBL
Match: B9HCD2_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_652366 PE=4 SV=1)

HSP 1 Score: 64.6994 bits (156), Expect = 1.607e-11
Identity = 27/41 (65.85%), Postives = 36/41 (87.80%), Query Frame = -1

Query:  487 LEIQPPMPRPQHGVLNHLYVQKGKSSPSVVALGTTHRYMAQ 609
            +EI PP  +PQH VLNHLY+QKG+S P+VVALG+THR++A+
Sbjct:  241 MEIPPPSSKPQHVVLNHLYMQKGRSGPAVVALGSTHRFLAK 281

HSP 2 Score: 28.8758 bits (63), Expect = 1.607e-11
Identity = 13/13 (100.00%), Postives = 13/13 (100.00%), Query Frame = -2

Query:  450 KYVTVVLYKSLQR 488
            KYVTVVLYKSLQR
Sbjct:  281 KYVTVVLYKSLQR 293

[Close]

BLAST of HO064247 vs. TrEMBL
Match: B9SA62_RICCO (Snf1-kinase beta subunit, plants, putative OS=Ricinus communis GN=RCOM_0861290 PE=4 SV=1)

HSP 1 Score: 62.003 bits (149), Expect = 9.985e-11
Identity = 25/41 (60.98%), Postives = 35/41 (85.37%), Query Frame = -1

Query:  487 LEIQPPMPRPQHGVLNHLYVQKGKSSPSVVALGTTHRYMAQ 609
            +E+  P+ RPQH VLNHLY+QKGK  P+VVALG+THR++++
Sbjct:  255 MEMPTPLSRPQHVVLNHLYIQKGKGGPAVVALGSTHRFLSK 295

HSP 2 Score: 28.8758 bits (63), Expect = 9.985e-11
Identity = 13/13 (100.00%), Postives = 13/13 (100.00%), Query Frame = -2

Query:  450 KYVTVVLYKSLQR 488
            KYVTVVLYKSLQR
Sbjct:  295 KYVTVVLYKSLQR 307

[Close]

BLAST of HO064247 vs. TAIR peptide
Match: AT4G16360.3 (| Symbols: | 5'-AMP-activated protein kinase beta-2 subunit protein | chr4:9245571-9246833 FORWARD LENGTH=261)

HSP 1 Score: 68.1662 bits (165), Expect = 1.080e-14
Identity = 29/42 (69.05%), Postives = 37/42 (88.10%), Query Frame = -1

Query:  487 NLEIQPPMPRPQHGVLNHLYVQKGKSSPSVVALGTTHRYMAQ 612
            N +I  P+PRPQH +LNHLY+QKGKS PSVVALG+THR++A+
Sbjct:  208 NPDIPSPLPRPQHVILNHLYMQKGKSGPSVVALGSTHRFLAK 249

HSP 2 Score: 28.8758 bits (63), Expect = 1.080e-14
Identity = 13/13 (100.00%), Postives = 13/13 (100.00%), Query Frame = -2

Query:  450 KYVTVVLYKSLQR 488
            KYVTVVLYKSLQR
Sbjct:  249 KYVTVVLYKSLQR 261

[Close]

BLAST of HO064247 vs. TAIR peptide
Match: AT4G16360.1 (| Symbols: | 5'-AMP-activated protein kinase beta-2 subunit protein | chr4:9245662-9246833 FORWARD LENGTH=259)

HSP 1 Score: 68.1662 bits (165), Expect = 1.080e-14
Identity = 29/42 (69.05%), Postives = 37/42 (88.10%), Query Frame = -1

Query:  487 NLEIQPPMPRPQHGVLNHLYVQKGKSSPSVVALGTTHRYMAQ 612
            N +I  P+PRPQH +LNHLY+QKGKS PSVVALG+THR++A+
Sbjct:  206 NPDIPSPLPRPQHVILNHLYMQKGKSGPSVVALGSTHRFLAK 247

HSP 2 Score: 28.8758 bits (63), Expect = 1.080e-14
Identity = 13/13 (100.00%), Postives = 13/13 (100.00%), Query Frame = -2

Query:  450 KYVTVVLYKSLQR 488
            KYVTVVLYKSLQR
Sbjct:  247 KYVTVVLYKSLQR 259

[Close]

BLAST of HO064247 vs. TAIR peptide
Match: AT4G16360.2 (| Symbols: | 5'-AMP-activated protein kinase beta-2 subunit protein | chr4:9245662-9246833 FORWARD LENGTH=258)

HSP 1 Score: 68.1662 bits (165), Expect = 1.080e-14
Identity = 29/42 (69.05%), Postives = 37/42 (88.10%), Query Frame = -1

Query:  487 NLEIQPPMPRPQHGVLNHLYVQKGKSSPSVVALGTTHRYMAQ 612
            N +I  P+PRPQH +LNHLY+QKGKS PSVVALG+THR++A+
Sbjct:  205 NPDIPSPLPRPQHVILNHLYMQKGKSGPSVVALGSTHRFLAK 246

HSP 2 Score: 28.8758 bits (63), Expect = 1.080e-14
Identity = 13/13 (100.00%), Postives = 13/13 (100.00%), Query Frame = -2

Query:  450 KYVTVVLYKSLQR 488
            KYVTVVLYKSLQR
Sbjct:  246 KYVTVVLYKSLQR 258

The following BLAST results are available for this feature:

BLAST of HO064247 vs. SwissProt
BLAST of HO064247 vs. TrEMBL
BLAST of HO064247 vs. TAIR peptide

BLAST of HO064247 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Swissprot)
Total hits: 1


Match Name	E-value	Identity	Description
KINB2_ARATH	1.053e-13	69.05	SNF1-related protein kinase regulatory subunit bet...	[more]

BLAST of HO064247 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
D7SSK4_VITVI	7.001e-13	75.61	Whole genome shotgun sequence of line PN40024, sca...	[more]
A5AP67_VITVI	7.004e-13	75.61	Putative uncharacterized protein OS=Vitis vinifera...	[more]
D7SSK2_VITVI	7.681e-13	75.61	Whole genome shotgun sequence of line PN40024, sca...	[more]
Q7XYX7_MEDTR	9.064e-13	70.45	AKIN beta2 OS=Medicago truncatula PE=2 SV=1	[more]
B9IH18_POPTR	1.535e-12	70.73	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
Q2V3H9_ARATH	1.536e-12	69.05	Uncharacterized protein At4g16360.2 OS=Arabidopsis...	[more]
D7M9W3_ARALY	1.536e-12	69.05	AMP-activated protein kinase OS=Arabidopsis lyrata...	[more]
C0Z307_ARATH	1.686e-12	69.05	AT4G16360 protein OS=Arabidopsis thaliana GN=AT4G1...	[more]
B9HCD2_POPTR	1.607e-11	65.85	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
B9SA62_RICCO	9.985e-11	60.98	Snf1-kinase beta subunit, plants, putative OS=Rici...	[more]

BLAST of HO064247 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide)
Total hits: 3


Match Name	E-value	Identity	Description
AT4G16360.3	1.080e-14	69.05	\| Symbols: \| 5'-AMP-activated protein kinase beta...	[more]
AT4G16360.1	1.080e-14	69.05	\| Symbols: \| 5'-AMP-activated protein kinase beta...	[more]
AT4G16360.2	1.080e-14	69.05	\| Symbols: \| 5'-AMP-activated protein kinase beta...	[more]

InterPro

Analysis Name: InterProScan analysis for Cicer arietinum unigene v1
Date Performed: 2010-12-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006828	5-AMP-activated protein kinase, beta subunit, interaction domain	PFAM	PF04739	AMPKBI	coord: 17..49 score: 9.3
None	No IPR available	PANTHER	PTHR10343	5'-AMP-ACTIVATED PROTEIN KINASE , BETA SUBUNIT	coord: 10..50 score: 1.4
None	No IPR available	PANTHER	PTHR10343:SF4	SNF1-KINASE BETA SUBUNIT, PLANTS	coord: 10..50 score: 1.4
None	No IPR available	SEG	seg	seg	coord: 44..58 score

Properties


Property Name	Value
Singlet in analysis	Cicer arietinum unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>HO064247 ID=HO064247; Name=HO064247; organism=Cicer arietinum; type=EST; length=639bp
CAGCAAAATAAAAAGACTTCTTTATACCAGCCAAAGTCAATTTACAGGAG
AATGAATCACAAACATCTCTAATACGTGATTAAACCCGCAACAAAAACCC
GAACCCAACCACCAGGACGCACAAGAAAACTGAAAAAATAAGATAGGGAA
GTAGTAAGAGAGATGCTAACAATGAAAATGCAAATATGGTGGATATGAAC
TGCAAAATAGCTTAACCACCAAATTGCTGTTCCAGCTATAAGTGATAAGA
AGAACATGTAAATTATTTTATCCTTTTGATCACTCAATCCAAATTCCTAG
ATAGAAGTATCACCAAATATGTAAGAACTGGAACTAAATCATCTGATAAT
TGGCCATTAAGTAATTGCATTATATGTAATACAATCTTGACTTCCAGGCA
GTGATCTCAATCTTAATGACTGGTCCATCTTATTTGTGTTGACGATCTAC
CTCTGCAAAGACTTGTAAAGCACCACAGTGACATATTTGGGCCATGTACC
GATGAGTTGTACCAAGGGCAACCACTGAAGGACTGCTCTTTCCTTTCTGC
ACGTAAAGATGATTGAGCACTCCATGTTGAGGTCTTGGCATAGGAGGTTG
GATTTCCAGATTTGTTGTACCTGCCGGCGGCCGCTCGAA

Annotated Terms

The following terms have been associated with this EST:

Vocabulary: **INTERPRO**
Term	Definition
IPR006828	ASC_dom

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