HO064706

EST Overview


Name	HO064706
Unique Name	HO064706
Type	EST
Organism	Cicer arietinum (chickpea)
Sequence length	551

Libraries


Library Name	Type
LIBEST_026411 Chickpea drought stressed cDNA SSH library AR2	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Cicer arietinum unigene v1	Cicer arietinum unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Cicer arietinum unigene v1	2010-12-27
Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide	2011-02-03
Homology Analysis: Cicer arietinum unigene v1 vs Swissprot	2010-12-28
Homology Analysis: Cicer arietinum unigene v1 vs Trembl	2010-12-28
InterProScan analysis for Cicer arietinum unigene v1	2010-12-29

Homology

[Close]

BLAST of HO064706 vs. SwissProt
Match: TCMO_CICAR (Trans-cinnamate 4-monooxygenase OS=Cicer arietinum GN=CYP73A19 PE=2 SV=2)

HSP 1 Score: 113.62 bits (283), Expect = 4.729e-26
Identity = 59/98 (60.20%), Postives = 70/98 (71.43%), Query Frame = -2

Query:  158 MDHLVLEQTLLNFFMTSVIAIAVPKPGSKRFELTSGTIPVPVFGNWRQKIDDLNLRNIIELARRVGSMILLIMGQRNLVVAS*PAMPKEVFHTQGGEF 451
            MD L+LE+TLL  F+ + IAI + K   KRF+L  G IPVPVFGNW Q  DDLN RN+ +LA+R G + LL MGQRNLVV S P + KEV HTQG EF
Sbjct:    1 MDLLLLEKTLLALFIAATIAITISKLRGKRFKLPPGPIPVPVFGNWLQVGDDLNHRNLTDLAKRFGDIFLLRMGQRNLVVVSSPELAKEVLHTQGVEF 98

HSP 2 Score: 24.6386 bits (52), Expect = 4.729e-26
Identity = 15/34 (44.12%), Postives = 17/34 (50.00%), Query Frame = -3

Query:   22 TGKGRDKVNTVGGEQNGNHKNHAGAVFTNKEVPQ 123
            TGKG+D V TV G      +      FTNK V Q
Sbjct:  111 TGKGQDMVFTVYGTLAEMRRIMTVPFFTNKVVQQ 144

[Close]

BLAST of HO064706 vs. SwissProt
Match: TCMO_GLYEC (Trans-cinnamate 4-monooxygenase OS=Glycyrrhiza echinata GN=CYP73A14 PE=2 SV=1)

HSP 1 Score: 113.62 bits (283), Expect = 8.010e-26
Identity = 57/98 (58.16%), Postives = 71/98 (72.45%), Query Frame = -2

Query:  158 MDHLVLEQTLLNFFMTSVIAIAVPKPGSKRFELTSGTIPVPVFGNWRQKIDDLNLRNIIELARRVGSMILLIMGQRNLVVAS*PAMPKEVFHTQGGEF 451
            MD L+LE+TLL  F+ ++ AIA+ K   +RF+L  G IPVP+FGNW Q  DDLN RN+ +LA+R G + LL MGQRNLVV S P + KEV HTQG EF
Sbjct:    1 MDLLLLEKTLLGLFIAAITAIAISKLRGRRFKLPPGPIPVPIFGNWLQVGDDLNHRNLTDLAKRFGDIFLLRMGQRNLVVVSSPELAKEVLHTQGVEF 98

HSP 2 Score: 23.8682 bits (50), Expect = 8.010e-26
Identity = 16/35 (45.71%), Postives = 18/35 (51.43%), Query Frame = -3

Query:   22 TGKGRDKVNTVGGEQ-NGNHKNHAGAVFTNKEVPQ 123
            TGKG+D V TV GE      +      FTNK V Q
Sbjct:  111 TGKGQDMVFTVYGEHWRKMRRIMTVPFFTNKVVQQ 145

[Close]

BLAST of HO064706 vs. SwissProt
Match: TCMO_PHAAU (Trans-cinnamate 4-monooxygenase OS=Phaseolus aureus GN=CYP73A2 PE=1 SV=1)

HSP 1 Score: 114.775 bits (286), Expect = 1.042e-25
Identity = 58/98 (59.18%), Postives = 71/98 (72.45%), Query Frame = -2

Query:  158 MDHLVLEQTLLNFFMTSVIAIAVPKPGSKRFELTSGTIPVPVFGNWRQKIDDLNLRNIIELARRVGSMILLIMGQRNLVVAS*PAMPKEVFHTQGGEF 451
            MD L+LE+TLL  F+ +V+AI V K   KRF+L  G +PVP+FGNW Q  DDLN RN+ +LA+R G + LL MGQRNLVV S P + KEV HTQG EF
Sbjct:    1 MDLLLLEKTLLGLFLAAVVAIVVSKLRGKRFKLPPGPLPVPIFGNWLQVGDDLNHRNLTQLAKRFGDIFLLRMGQRNLVVVSSPDLAKEVLHTQGVEF 98

HSP 2 Score: 22.3274 bits (46), Expect = 1.042e-25
Identity = 15/35 (42.86%), Postives = 18/35 (51.43%), Query Frame = -3

Query:   22 TGKGRDKVNTVGGEQ-NGNHKNHAGAVFTNKEVPQ 123
            TG+G+D V TV GE      +      FTNK V Q
Sbjct:  111 TGEGQDMVFTVYGEHWRKMRRIMTVPFFTNKVVQQ 145

[Close]

BLAST of HO064706 vs. SwissProt
Match: TCMO_POPTM (Trans-cinnamate 4-monooxygenase OS=Populus tremuloides GN=CYP73A13 PE=2 SV=1)

HSP 1 Score: 111.309 bits (277), Expect = 3.892e-25
Identity = 57/98 (58.16%), Postives = 71/98 (72.45%), Query Frame = -2

Query:  158 MDHLVLEQTLLNFFMTSVIAIAVPKPGSKRFELTSGTIPVPVFGNWRQKIDDLNLRNIIELARRVGSMILLIMGQRNLVVAS*PAMPKEVFHTQGGEF 451
            MD L+LE+TLL  F+  ++AI V K   KRF+L  G +PVPVFGNW Q  DDLN RN+ +LA++ G ++LL MGQRNLVV S P + KEV HTQG EF
Sbjct:    1 MDLLLLEKTLLGSFVAILVAILVSKLRGKRFKLPPGPLPVPVFGNWLQVGDDLNHRNLTDLAKKFGDILLLRMGQRNLVVVSSPELSKEVLHTQGVEF 98

HSP 2 Score: 23.8682 bits (50), Expect = 3.892e-25
Identity = 16/35 (45.71%), Postives = 18/35 (51.43%), Query Frame = -3

Query:   22 TGKGRDKVNTVGGEQ-NGNHKNHAGAVFTNKEVPQ 123
            TGKG+D V TV GE      +      FTNK V Q
Sbjct:  111 TGKGQDMVFTVYGEHWRKMRRIMTVPFFTNKVVQQ 145

[Close]

BLAST of HO064706 vs. SwissProt
Match: TCMO_SOYBN (Trans-cinnamate 4-monooxygenase OS=Glycine max GN=CYP73A11 PE=2 SV=1)

HSP 1 Score: 110.153 bits (274), Expect = 8.578e-25
Identity = 54/98 (55.10%), Postives = 71/98 (72.45%), Query Frame = -2

Query:  158 MDHLVLEQTLLNFFMTSVIAIAVPKPGSKRFELTSGTIPVPVFGNWRQKIDDLNLRNIIELARRVGSMILLIMGQRNLVVAS*PAMPKEVFHTQGGEF 451
            MD L+LE+TL+  F+ +V+AIAV     ++F+L  G +PVP+FGNW Q  DDLN RN+ +LA++ G + LL MGQRNLVV S P + KEV HTQG EF
Sbjct:    1 MDLLLLEKTLIGLFLAAVVAIAVSTLRGRKFKLPPGPLPVPIFGNWLQVGDDLNHRNLTDLAKKFGDIFLLRMGQRNLVVVSSPELAKEVLHTQGVEF 98

HSP 2 Score: 23.8682 bits (50), Expect = 8.578e-25
Identity = 16/35 (45.71%), Postives = 18/35 (51.43%), Query Frame = -3

Query:   22 TGKGRDKVNTVGGEQ-NGNHKNHAGAVFTNKEVPQ 123
            TGKG+D V TV GE      +      FTNK V Q
Sbjct:  111 TGKGQDMVFTVYGEHWRKMRRIMTVPFFTNKVVQQ 145

[Close]

BLAST of HO064706 vs. SwissProt
Match: TCMO_POPKI (Trans-cinnamate 4-monooxygenase OS=Populus kitakamiensis GN=CYP73A16 PE=2 SV=1)

HSP 1 Score: 110.153 bits (274), Expect = 8.578e-25
Identity = 57/98 (58.16%), Postives = 70/98 (71.43%), Query Frame = -2

Query:  158 MDHLVLEQTLLNFFMTSVIAIAVPKPGSKRFELTSGTIPVPVFGNWRQKIDDLNLRNIIELARRVGSMILLIMGQRNLVVAS*PAMPKEVFHTQGGEF 451
            MD L+LE+TLL  F+  ++AI V K   KRF+L  G +PVPVFGNW Q  DDLN RN+ +LA++ G + LL MGQRNLVV S P + KEV HTQG EF
Sbjct:    1 MDLLLLEKTLLGSFVAVLVAILVSKLRGKRFKLPPGPLPVPVFGNWLQVGDDLNHRNLTDLAKKFGDIFLLRMGQRNLVVVSSPDLSKEVLHTQGVEF 98

HSP 2 Score: 23.8682 bits (50), Expect = 8.578e-25
Identity = 16/35 (45.71%), Postives = 18/35 (51.43%), Query Frame = -3

Query:   22 TGKGRDKVNTVGGEQ-NGNHKNHAGAVFTNKEVPQ 123
            TGKG+D V TV GE      +      FTNK V Q
Sbjct:  111 TGKGQDMVFTVYGEHWRKMRRIMTVPFFTNKVVQQ 145

[Close]

BLAST of HO064706 vs. SwissProt
Match: TCMO_HELTU (Trans-cinnamate 4-monooxygenase OS=Helianthus tuberosus GN=CYP73A1 PE=1 SV=1)

HSP 1 Score: 106.686 bits (265), Expect = 9.177e-24
Identity = 54/98 (55.10%), Postives = 69/98 (70.41%), Query Frame = -2

Query:  158 MDHLVLEQTLLNFFMTSVIAIAVPKPGSKRFELTSGTIPVPVFGNWRQKIDDLNLRNIIELARRVGSMILLIMGQRNLVVAS*PAMPKEVFHTQGGEF 451
            MD L++E+TL+  F   + AI + K   K+F+L  G IPVP+FGNW Q  DDLN RN+ +LA+R G ++LL MGQRNLVV S P + KEV HTQG EF
Sbjct:    1 MDLLLIEKTLVALFAAIIGAILISKLRGKKFKLPPGPIPVPIFGNWLQVGDDLNHRNLTDLAKRFGEILLLRMGQRNLVVVSSPELAKEVLHTQGVEF 98

HSP 2 Score: 23.8682 bits (50), Expect = 9.177e-24
Identity = 16/35 (45.71%), Postives = 18/35 (51.43%), Query Frame = -3

Query:   22 TGKGRDKVNTVGGEQ-NGNHKNHAGAVFTNKEVPQ 123
            TGKG+D V TV GE      +      FTNK V Q
Sbjct:  111 TGKGQDMVFTVYGEHWRKMRRIMTVPFFTNKVVQQ 145

[Close]

BLAST of HO064706 vs. SwissProt
Match: TCMO_CATRO (Trans-cinnamate 4-monooxygenase OS=Catharanthus roseus GN=CYP73A4 PE=2 SV=1)

HSP 1 Score: 106.301 bits (264), Expect = 1.194e-23
Identity = 55/98 (56.12%), Postives = 67/98 (68.37%), Query Frame = -2

Query:  158 MDHLVLEQTLLNFFMTSVIAIAVPKPGSKRFELTSGTIPVPVFGNWRQKIDDLNLRNIIELARRVGSMILLIMGQRNLVVAS*PAMPKEVFHTQGGEF 451
            MD L+LE+TLL  F   ++A  V K   K+F+L  G IPVPVFGNW Q  DDLN RN+ + A++ G + LL MGQRNLVV S P + KEV HTQG EF
Sbjct:    1 MDLLLLEKTLLGLFAAIIVASIVSKLRGKKFKLPPGPIPVPVFGNWLQVGDDLNHRNLSDYAKKFGEIFLLRMGQRNLVVVSSPELAKEVLHTQGVEF 98

HSP 2 Score: 23.8682 bits (50), Expect = 1.194e-23
Identity = 16/35 (45.71%), Postives = 18/35 (51.43%), Query Frame = -3

Query:   22 TGKGRDKVNTVGGEQ-NGNHKNHAGAVFTNKEVPQ 123
            TGKG+D V TV GE      +      FTNK V Q
Sbjct:  111 TGKGQDMVFTVYGEHWRKMRRIMTVPFFTNKVVQQ 145

[Close]

BLAST of HO064706 vs. SwissProt
Match: TCMO_ZINEL (Trans-cinnamate 4-monooxygenase OS=Zinnia elegans GN=CYP73A12 PE=2 SV=1)

HSP 1 Score: 105.531 bits (262), Expect = 2.021e-23
Identity = 53/98 (54.08%), Postives = 68/98 (69.39%), Query Frame = -2

Query:  158 MDHLVLEQTLLNFFMTSVIAIAVPKPGSKRFELTSGTIPVPVFGNWRQKIDDLNLRNIIELARRVGSMILLIMGQRNLVVAS*PAMPKEVFHTQGGEF 451
            MD L++E+TLL  F   + +I + K   KRF+L  G +PVP+FGNW Q  DDLN RN+ +LA++ G + LL MGQRNLVV S P + KEV HTQG EF
Sbjct:    1 MDLLLVEKTLLALFAAIIASIFISKLRGKRFKLPPGPVPVPIFGNWLQVGDDLNHRNLTDLAKKFGEIFLLRMGQRNLVVVSSPNLAKEVLHTQGVEF 98

HSP 2 Score: 23.8682 bits (50), Expect = 2.021e-23
Identity = 16/35 (45.71%), Postives = 18/35 (51.43%), Query Frame = -3

Query:   22 TGKGRDKVNTVGGEQ-NGNHKNHAGAVFTNKEVPQ 123
            TGKG+D V TV GE      +      FTNK V Q
Sbjct:  111 TGKGQDMVFTVYGEHWRKMRRIMTVPFFTNKVVQQ 145

[Close]

BLAST of HO064706 vs. SwissProt
Match: TCMO_MEDSA (Trans-cinnamate 4-monooxygenase OS=Medicago sativa GN=CYP73A3 PE=2 SV=1)

HSP 1 Score: 104.375 bits (259), Expect = 4.452e-23
Identity = 54/98 (55.10%), Postives = 67/98 (68.37%), Query Frame = -2

Query:  158 MDHLVLEQTLLNFFMTSVIAIAVPKPGSKRFELTSGTIPVPVFGNWRQKIDDLNLRNIIELARRVGSMILLIMGQRNLVVAS*PAMPKEVFHTQGGEF 451
            MD L+LE+TLL  F+ + IA+ + K   KRF+L  G IPVP+FG W Q  DDLN RN+ + A+R G + LL MGQRNLVV S P + KEV HTQ  EF
Sbjct:    1 MDLLLLEKTLLALFIAATIAVTISKLRGKRFKLPPGPIPVPIFGYWLQVGDDLNHRNLTDYAKRFGEIFLLRMGQRNLVVVSSPELAKEVLHTQCVEF 98

HSP 2 Score: 23.8682 bits (50), Expect = 4.452e-23
Identity = 16/35 (45.71%), Postives = 18/35 (51.43%), Query Frame = -3

Query:   22 TGKGRDKVNTVGGEQ-NGNHKNHAGAVFTNKEVPQ 123
            TGKG+D V TV GE      +      FTNK V Q
Sbjct:  111 TGKGQDMVFTVYGEHWRKMRRIMTVPFFTNKVVQQ 145

[Close]

BLAST of HO064706 vs. TrEMBL
Match: B2LSE1_TRIPR (Cinnamic acid 4-hydroxylase OS=Trifolium pratense GN=CYP73 PE=2 SV=1)

HSP 1 Score: 116.316 bits (290), Expect = 1.714e-25
Identity = 58/98 (59.18%), Postives = 73/98 (74.49%), Query Frame = -2

Query:  158 MDHLVLEQTLLNFFMTSVIAIAVPKPGSKRFELTSGTIPVPVFGNWRQKIDDLNLRNIIELARRVGSMILLIMGQRNLVVAS*PAMPKEVFHTQGGEF 451
            MD L+LE+TLL+ F+ ++IAI + K   KRF+L  G IPVP+FGNW Q  DDLN RN+ +LA+R G ++LL MGQRNLVV S P + KEV HTQG EF
Sbjct:    1 MDLLLLEKTLLSLFIAAIIAITISKLRGKRFKLPPGPIPVPIFGNWLQVGDDLNHRNLTDLAKRFGEILLLRMGQRNLVVVSSPELAKEVLHTQGVEF 98

HSP 2 Score: 23.8682 bits (50), Expect = 1.714e-25
Identity = 16/35 (45.71%), Postives = 18/35 (51.43%), Query Frame = -3

Query:   22 TGKGRDKVNTVGGEQ-NGNHKNHAGAVFTNKEVPQ 123
            TGKG+D V TV GE      +      FTNK V Q
Sbjct:  111 TGKGQDMVFTVYGEHWRKMRRIMTVPFFTNKVVQQ 145

[Close]

BLAST of HO064706 vs. TrEMBL
Match: B2LSD9_TRIPR (Cinnamic acid 4-hydroxylase OS=Trifolium pratense GN=CYP73 PE=2 SV=1)

HSP 1 Score: 116.316 bits (290), Expect = 1.714e-25
Identity = 58/98 (59.18%), Postives = 73/98 (74.49%), Query Frame = -2

Query:  158 MDHLVLEQTLLNFFMTSVIAIAVPKPGSKRFELTSGTIPVPVFGNWRQKIDDLNLRNIIELARRVGSMILLIMGQRNLVVAS*PAMPKEVFHTQGGEF 451
            MD L+LE+TLL+ F+ ++IAI + K   KRF+L  G IPVP+FGNW Q  DDLN RN+ +LA+R G ++LL MGQRNLVV S P + KEV HTQG EF
Sbjct:    1 MDLLLLEKTLLSLFIAAIIAITISKLRGKRFKLPPGPIPVPIFGNWLQVGDDLNHRNLTDLAKRFGEILLLRMGQRNLVVVSSPELAKEVLHTQGVEF 98

HSP 2 Score: 23.8682 bits (50), Expect = 1.714e-25
Identity = 16/35 (45.71%), Postives = 18/35 (51.43%), Query Frame = -3

Query:   22 TGKGRDKVNTVGGEQ-NGNHKNHAGAVFTNKEVPQ 123
            TGKG+D V TV GE      +      FTNK V Q
Sbjct:  111 TGKGQDMVFTVYGEHWRKMRRIMTVPFFTNKVVQQ 145

[Close]

BLAST of HO064706 vs. TrEMBL
Match: D2K757_LEUGL (Cinnamate 4-hydroxylase OS=Leucaena glauca GN=C4H PE=2 SV=1)

HSP 1 Score: 114.005 bits (284), Expect = 8.334e-25
Identity = 57/98 (58.16%), Postives = 72/98 (73.47%), Query Frame = -2

Query:  158 MDHLVLEQTLLNFFMTSVIAIAVPKPGSKRFELTSGTIPVPVFGNWRQKIDDLNLRNIIELARRVGSMILLIMGQRNLVVAS*PAMPKEVFHTQGGEF 451
            MD L+LE+TLL  F+ +V+AIAV K   KRF+L  G +PVP+FGNW Q  DDLN RN+ +LA++ G + LL MGQRNL+V S P + KEV HTQG EF
Sbjct:    1 MDLLLLEKTLLGLFVAAVVAIAVSKLRGKRFKLPPGPLPVPIFGNWLQVGDDLNHRNLTDLAKKFGDIFLLRMGQRNLLVVSSPELAKEVLHTQGVEF 98

HSP 2 Score: 23.8682 bits (50), Expect = 8.334e-25
Identity = 16/35 (45.71%), Postives = 18/35 (51.43%), Query Frame = -3

Query:   22 TGKGRDKVNTVGGEQ-NGNHKNHAGAVFTNKEVPQ 123
            TGKG+D V TV GE      +      FTNK V Q
Sbjct:  111 TGKGQDMVFTVYGEHWRKMRRIMTVPFFTNKVVQQ 145

[Close]

BLAST of HO064706 vs. TrEMBL
Match: Q2MJ09_MEDTR (Cytochrome P450 monooxygenase CYP73A3 OS=Medicago truncatula GN=CYP73A3 PE=2 SV=1)

HSP 1 Score: 112.849 bits (281), Expect = 1.837e-24
Identity = 58/98 (59.18%), Postives = 69/98 (70.41%), Query Frame = -2

Query:  158 MDHLVLEQTLLNFFMTSVIAIAVPKPGSKRFELTSGTIPVPVFGNWRQKIDDLNLRNIIELARRVGSMILLIMGQRNLVVAS*PAMPKEVFHTQGGEF 451
            MD L+LE+TLL  F+ + IAI + K   KRF+L  G IPVP+FGNW Q  DDLN RN+ + A+R G M LL MGQRNLVV S P + KEV HTQG EF
Sbjct:    1 MDLLLLEKTLLALFIAATIAITISKLRGKRFKLPPGPIPVPIFGNWLQVGDDLNHRNLTDYAKRFGEMFLLRMGQRNLVVVSSPELAKEVLHTQGVEF 98

HSP 2 Score: 23.8682 bits (50), Expect = 1.837e-24
Identity = 16/35 (45.71%), Postives = 18/35 (51.43%), Query Frame = -3

Query:   22 TGKGRDKVNTVGGEQ-NGNHKNHAGAVFTNKEVPQ 123
            TGKG+D V TV GE      +      FTNK V Q
Sbjct:  111 TGKGQDMVFTVYGEHWRKMRRIMTVPFFTNKVVQQ 145

[Close]

BLAST of HO064706 vs. TrEMBL
Match: B9SD83_RICCO (Cinnamate 4-hydroxylase, putative OS=Ricinus communis GN=RCOM_1164350 PE=3 SV=1)

HSP 1 Score: 112.464 bits (280), Expect = 2.391e-24
Identity = 56/98 (57.14%), Postives = 70/98 (71.43%), Query Frame = -2

Query:  158 MDHLVLEQTLLNFFMTSVIAIAVPKPGSKRFELTSGTIPVPVFGNWRQKIDDLNLRNIIELARRVGSMILLIMGQRNLVVAS*PAMPKEVFHTQGGEF 451
            MD L+LE+TLL  F   ++AI + K   KRF+L  G +PVPVFGNW Q  DDLN RN+ +LA++ G ++LL MGQRNLVV S P + KEV HTQG EF
Sbjct:    1 MDPLLLEKTLLGLFAAVIVAIVISKLRGKRFKLPPGPLPVPVFGNWLQVGDDLNQRNLADLAKKFGDILLLRMGQRNLVVVSSPDLAKEVLHTQGVEF 98

HSP 2 Score: 23.8682 bits (50), Expect = 2.391e-24
Identity = 16/35 (45.71%), Postives = 18/35 (51.43%), Query Frame = -3

Query:   22 TGKGRDKVNTVGGEQ-NGNHKNHAGAVFTNKEVPQ 123
            TGKG+D V TV GE      +      FTNK V Q
Sbjct:  111 TGKGQDMVFTVYGEHWRKMRRIMTVPFFTNKVVQQ 145

[Close]

BLAST of HO064706 vs. TrEMBL
Match: Q2LAD8_SOLTU (Cinnamic acid 4-hydroxylase OS=Solanum tuberosum PE=3 SV=1)

HSP 1 Score: 112.079 bits (279), Expect = 2.391e-24
Identity = 59/98 (60.20%), Postives = 69/98 (70.41%), Query Frame = -2

Query:  158 MDHLVLEQTLLNFFMTSVIAIAVPKPGSKRFELTSGTIPVPVFGNWRQKIDDLNLRNIIELARRVGSMILLIMGQRNLVVAS*PAMPKEVFHTQGGEF 451
            MD L+LE+TL+  F   +IAI V K  SKRF+L  G IPVPVFGNW Q  DDLN RN+ E A++ G + LL MGQRNLVV S P + KEV HTQG EF
Sbjct:    1 MDLLLLEKTLIGLFFAILIAIIVSKLRSKRFKLPPGPIPVPVFGNWLQVGDDLNHRNLTEYAKKFGDVFLLRMGQRNLVVVSSPELAKEVLHTQGVEF 98

HSP 2 Score: 24.2534 bits (51), Expect = 2.391e-24
Identity = 17/38 (44.74%), Postives = 19/38 (50.00%), Query Frame = -3

Query:   13 TGKGRDKVNTVGGEQ-NGNHKNHAGAVFTNKEVPQPPG 123
            TGKG+D V TV GE      +      FTNK V Q  G
Sbjct:  111 TGKGQDMVFTVYGEHWRKMRRIMTVPFFTNKVVQQYRG 148

[Close]

BLAST of HO064706 vs. TrEMBL
Match: Q0QDS3_9BORA (Cinnamic acid 4-hydroxylase OS=Arnebia euchroma PE=2 SV=2)

HSP 1 Score: 111.694 bits (278), Expect = 4.050e-24
Identity = 56/98 (57.14%), Postives = 69/98 (70.41%), Query Frame = -2

Query:  158 MDHLVLEQTLLNFFMTSVIAIAVPKPGSKRFELTSGTIPVPVFGNWRQKIDDLNLRNIIELARRVGSMILLIMGQRNLVVAS*PAMPKEVFHTQGGEF 451
            MD L+LE+ LL  F + +IAI + K G K+F+L  G IPVP+FGNW Q  DDLN RN+ E A++ G + LL MGQRNLVV S P + KEV HTQG EF
Sbjct:    1 MDLLLLEKALLGLFFSVIIAIVISKLGGKKFKLPPGPIPVPIFGNWLQVGDDLNHRNLTEYAKKFGEIFLLRMGQRNLVVVSSPDLAKEVLHTQGVEF 98

HSP 2 Score: 23.8682 bits (50), Expect = 4.050e-24
Identity = 16/35 (45.71%), Postives = 18/35 (51.43%), Query Frame = -3

Query:   22 TGKGRDKVNTVGGEQ-NGNHKNHAGAVFTNKEVPQ 123
            TGKG+D V TV GE      +      FTNK V Q
Sbjct:  111 TGKGQDMVFTVYGEHWRKMRRIMTVPFFTNKVVQQ 145

[Close]

BLAST of HO064706 vs. TrEMBL
Match: Q17UC0_CUCSA (Cinnamate-4-hydroxylase OS=Cucumis sativus GN=c4H PE=2 SV=1)

HSP 1 Score: 111.309 bits (277), Expect = 5.271e-24
Identity = 56/98 (57.14%), Postives = 71/98 (72.45%), Query Frame = -2

Query:  158 MDHLVLEQTLLNFFMTSVIAIAVPKPGSKRFELTSGTIPVPVFGNWRQKIDDLNLRNIIELARRVGSMILLIMGQRNLVVAS*PAMPKEVFHTQGGEF 451
            MD L+LE+TLL  F++ V+AIA+ K   KRF+   G +PVP+FGNW Q  DDLN RN+ +LA++ G + LL MGQRNLVV S P + KEV HTQG EF
Sbjct:    1 MDLLLLEKTLLGLFLSVVLAIAISKLRGKRFKPPPGPLPVPIFGNWLQVGDDLNHRNLTDLAKKFGDIFLLRMGQRNLVVVSSPELAKEVLHTQGVEF 98

HSP 2 Score: 23.8682 bits (50), Expect = 5.271e-24
Identity = 16/35 (45.71%), Postives = 18/35 (51.43%), Query Frame = -3

Query:   22 TGKGRDKVNTVGGEQ-NGNHKNHAGAVFTNKEVPQ 123
            TGKG+D V TV GE      +      FTNK V Q
Sbjct:  111 TGKGQDMVFTVYGEHWRKMRRIMTVPFFTNKVVQQ 145

[Close]

BLAST of HO064706 vs. TrEMBL
Match: Q9AXP9_9ROSI (Cinnamate 4-hydroxylase OS=Populus trichocarpa x Populus deltoides GN=C4H PE=2 SV=1)

HSP 1 Score: 110.923 bits (276), Expect = 6.859e-24
Identity = 58/98 (59.18%), Postives = 70/98 (71.43%), Query Frame = -2

Query:  158 MDHLVLEQTLLNFFMTSVIAIAVPKPGSKRFELTSGTIPVPVFGNWRQKIDDLNLRNIIELARRVGSMILLIMGQRNLVVAS*PAMPKEVFHTQGGEF 451
            MD L+LE+TLL  F+  ++AI V K   KRF+L  G IPVPVFGNW Q  DDLN RN+ +LA++ G + LL MGQRNLVV S P + KEV HTQG EF
Sbjct:    1 MDLLLLEKTLLGSFVAILVAILVSKLRGKRFKLPPGPIPVPVFGNWLQVGDDLNHRNLTDLAKKFGDIFLLRMGQRNLVVVSSPDLSKEVLHTQGVEF 98

HSP 2 Score: 23.8682 bits (50), Expect = 6.859e-24
Identity = 16/35 (45.71%), Postives = 18/35 (51.43%), Query Frame = -3

Query:   22 TGKGRDKVNTVGGEQ-NGNHKNHAGAVFTNKEVPQ 123
            TGKG+D V TV GE      +      FTNK V Q
Sbjct:  111 TGKGQDMVFTVYGEHWRKMRRIMTVPFFTNKVVQQ 145

[Close]

BLAST of HO064706 vs. TrEMBL
Match: Q0Q5Z7_POPTM (Trans-cinnamate 4-hydroxylase OS=Populus tremuloides GN=C4H1-1 PE=2 SV=1)

HSP 1 Score: 110.923 bits (276), Expect = 6.859e-24
Identity = 57/98 (58.16%), Postives = 71/98 (72.45%), Query Frame = -2

Query:  158 MDHLVLEQTLLNFFMTSVIAIAVPKPGSKRFELTSGTIPVPVFGNWRQKIDDLNLRNIIELARRVGSMILLIMGQRNLVVAS*PAMPKEVFHTQGGEF 451
            MD L+LE+TLL  F+  ++AI V K   KRF+L  G +PVPVFGNW Q  DDLN RN+ +LA++ G ++LL MGQRNLVV S P + KEV HTQG EF
Sbjct:    1 MDLLLLEKTLLGSFVAILVAILVSKLRGKRFKLPPGPLPVPVFGNWLQVGDDLNHRNLTDLAKKFGDILLLRMGQRNLVVVSSPDLAKEVLHTQGVEF 98

HSP 2 Score: 23.8682 bits (50), Expect = 6.859e-24
Identity = 16/35 (45.71%), Postives = 18/35 (51.43%), Query Frame = -3

Query:   22 TGKGRDKVNTVGGEQ-NGNHKNHAGAVFTNKEVPQ 123
            TGKG+D V TV GE      +      FTNK V Q
Sbjct:  111 TGKGQDMVFTVYGEHWRKMRRIMTVPFFTNKVVQQ 145

[Close]

BLAST of HO064706 vs. TAIR peptide
Match: AT2G30490.1 (| Symbols: ATC4H, C4H, CYP73A5, REF3 | cinnamate-4-hydroxylase | chr2:12993861-12995683 REVERSE LENGTH=505)

HSP 1 Score: 98.2117 bits (243), Expect = 3.219e-22
Identity = 48/98 (48.98%), Postives = 67/98 (68.37%), Query Frame = -2

Query:  158 MDHLVLEQTLLNFFMTSVIAIAVPKPGSKRFELTSGTIPVPVFGNWRQKIDDLNLRNIIELARRVGSMILLIMGQRNLVVAS*PAMPKEVFHTQGGEF 451
            MD L+LE++L+  F+  ++A  + K   K+ +L  G IP+P+FGNW Q  DDLN RN+++ A++ G + LL MGQRNLVV S P + KEV  TQG EF
Sbjct:    1 MDLLLLEKSLIAVFVAVILATVISKLRGKKLKLPPGPIPIPIFGNWLQVGDDLNHRNLVDYAKKFGDLFLLRMGQRNLVVVSSPDLTKEVLLTQGVEF 98

HSP 2 Score: 23.8682 bits (50), Expect = 3.219e-22
Identity = 16/35 (45.71%), Postives = 18/35 (51.43%), Query Frame = -3

Query:   22 TGKGRDKVNTVGGEQ-NGNHKNHAGAVFTNKEVPQ 123
            TGKG+D V TV GE      +      FTNK V Q
Sbjct:  111 TGKGQDMVFTVYGEHWRKMRRIMTVPFFTNKVVQQ 145

The following BLAST results are available for this feature:

BLAST of HO064706 vs. SwissProt
BLAST of HO064706 vs. TrEMBL
BLAST of HO064706 vs. TAIR peptide

BLAST of HO064706 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Swissprot)
Total hits: 10


Match Name	E-value	Identity	Description
TCMO_CICAR	4.729e-26	60.20	Trans-cinnamate 4-monooxygenase OS=Cicer arietinum...	[more]
TCMO_GLYEC	8.010e-26	58.16	Trans-cinnamate 4-monooxygenase OS=Glycyrrhiza ech...	[more]
TCMO_PHAAU	1.042e-25	59.18	Trans-cinnamate 4-monooxygenase OS=Phaseolus aureu...	[more]
TCMO_POPTM	3.892e-25	58.16	Trans-cinnamate 4-monooxygenase OS=Populus tremulo...	[more]
TCMO_SOYBN	8.578e-25	55.10	Trans-cinnamate 4-monooxygenase OS=Glycine max GN=...	[more]
TCMO_POPKI	8.578e-25	58.16	Trans-cinnamate 4-monooxygenase OS=Populus kitakam...	[more]
TCMO_HELTU	9.177e-24	55.10	Trans-cinnamate 4-monooxygenase OS=Helianthus tube...	[more]
TCMO_CATRO	1.194e-23	56.12	Trans-cinnamate 4-monooxygenase OS=Catharanthus ro...	[more]
TCMO_ZINEL	2.021e-23	54.08	Trans-cinnamate 4-monooxygenase OS=Zinnia elegans ...	[more]
TCMO_MEDSA	4.452e-23	55.10	Trans-cinnamate 4-monooxygenase OS=Medicago sativa...	[more]

BLAST of HO064706 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
B2LSE1_TRIPR	1.714e-25	59.18	Cinnamic acid 4-hydroxylase OS=Trifolium pratense ...	[more]
B2LSD9_TRIPR	1.714e-25	59.18	Cinnamic acid 4-hydroxylase OS=Trifolium pratense ...	[more]
D2K757_LEUGL	8.334e-25	58.16	Cinnamate 4-hydroxylase OS=Leucaena glauca GN=C4H ...	[more]
Q2MJ09_MEDTR	1.837e-24	59.18	Cytochrome P450 monooxygenase CYP73A3 OS=Medicago ...	[more]
B9SD83_RICCO	2.391e-24	57.14	Cinnamate 4-hydroxylase, putative OS=Ricinus commu...	[more]
Q2LAD8_SOLTU	2.391e-24	60.20	Cinnamic acid 4-hydroxylase OS=Solanum tuberosum P...	[more]
Q0QDS3_9BORA	4.050e-24	57.14	Cinnamic acid 4-hydroxylase OS=Arnebia euchroma PE...	[more]
Q17UC0_CUCSA	5.271e-24	57.14	Cinnamate-4-hydroxylase OS=Cucumis sativus GN=c4H ...	[more]
Q9AXP9_9ROSI	6.859e-24	59.18	Cinnamate 4-hydroxylase OS=Populus trichocarpa x P...	[more]
Q0Q5Z7_POPTM	6.859e-24	58.16	Trans-cinnamate 4-hydroxylase OS=Populus tremuloid...	[more]

BLAST of HO064706 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide)
Total hits: 1


Match Name	E-value	Identity	Description
AT2G30490.1	3.219e-22	48.98	\| Symbols: ATC4H, C4H, CYP73A5, REF3 \| cinnamate-4...	[more]

InterPro

Analysis Name: InterProScan analysis for Cicer arietinum unigene v1
Date Performed: 2010-12-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	SEG	seg	seg	coord: 2..7 score
None	No IPR available	PANTHER	PTHR19383:SF33	CINNAMATE 4-HYDROXYLASE	coord: 39..115 score: 1.4
None	No IPR available	SEG	seg	seg	coord: 37..53 score
IPR001128	Cytochrome P450	PANTHER	PTHR19383	CYTOCHROME P450	coord: 39..115 score: 1.4

Properties


Property Name	Value
Singlet in analysis	Cicer arietinum unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>HO064706 ID=HO064706; Name=HO064706; organism=Cicer arietinum; type=EST; length=551bp
TACGGGGGCCGGGCCGGGGGGCTGTGGAACCTCTTTGTTTGTGAAAACGG
CACCGGCATGATTCTTGTGGTTTCCGTTTTGTTCACCGCCTACGGTGTTA
ACCTTATCCCGTCCTTTTCCGGTGAGATATCAACAACAACTCTCCTGGCT
CGGGATCGAATTCTCCACCTTGAGTGTGGAAAACCTCTTTTGGCATTGCA
GGTTATGATGCTACGACGAGGTTTCTTTGACCCATTATAAGGAGGATCAT
ACTCCCAACCCGCCTTGCTAATTCGATGATATTACGGAGGTTGAGATCAT
CGATCTTTTGTCGCCAGTTACCGAAGACGGGGACTGGGATTGTACCTGAT
GTAAGTTCGAATCTTTTGCTACCTGGTTTTGGAACTGCGATGGCGATCAC
GCTAGTCATGAAAAAGTTTAAGAGGGTCTGTTCCAATACCAGATGATCCA
TTGTTGGAAATGAATGGTGTTTTTTAGGAGATTGTCCCCCGCGAACCTGC
CCGGTCGGCCGCTCCAAGTCACTAGCGAATTCCTGGGCGACTGTGTGATC
C

Annotated Terms

The following terms have been associated with this EST:

Vocabulary: **Molecular Function**
Term	Definition
GO:0004497	monooxygenase activity
GO:0005506	iron ion binding
GO:0009055	electron transfer activity
GO:0020037	heme binding

Vocabulary: **INTERPRO**
Term	Definition
IPR001128	Cyt_P450

Search form