HO064047

EST Overview


Name	HO064047
Unique Name	HO064047
Type	EST
Organism	Cicer arietinum (chickpea)
Sequence length	670

Libraries


Library Name	Type
LIBEST_026411 Chickpea drought stressed cDNA SSH library AR2	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Cicer arietinum unigene v1	Cicer arietinum unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Cicer arietinum unigene v1	2010-12-27
Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide	2011-02-03
Homology Analysis: Cicer arietinum unigene v1 vs Trembl	2010-12-28
InterProScan analysis for Cicer arietinum unigene v1	2010-12-29

Homology

[Close]

BLAST of HO064047 vs. TrEMBL
Match: B9MY26_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_594508 PE=4 SV=1)

HSP 1 Score: 139.043 bits (349), Expect = 2.014e-33
Identity = 63/110 (57.27%), Postives = 85/110 (77.27%), Query Frame = -2

Query:  301 FSWKSMWRLLSDVFDVEFVELDEKDE-FDLVELMRDKGHVWDMIVEKYGLHKTKLDEIACYEAMVPVLRFEFQHVSSMNKSKDYGFFGYADAFKSIRFWVNKMKEMKLIP 627
            F+WKS+W +L +VFDVEFV  +E DE FD + +M+ KG VWD IVEKYGL +TK+++I C+EA+  VL F FQHV SMNKS++ GF G+AD  KSI  WV ++++MK+IP
Sbjct:  268 FTWKSLWGVLCEVFDVEFVAYEENDEKFDCLAMMKGKGKVWDEIVEKYGLFETKMEDITCFEALNVVLHFGFQHVCSMNKSRESGFQGFADTLKSIPMWVGRLRDMKIIP 377

HSP 2 Score: 28.4906 bits (62), Expect = 2.014e-33
Identity = 11/20 (55.00%), Postives = 14/20 (70.00%), Query Frame = -3

Query:  603 QNEAFNCTNGDVLVGRVCGG 662
            +N+AFNCTNGDV   +   G
Sbjct:  256 KNQAFNCTNGDVFTWKSLWG 275

[Close]

BLAST of HO064047 vs. TrEMBL
Match: A9PJM4_9ROSI (Putative uncharacterized protein OS=Populus trichocarpa x Populus deltoides PE=2 SV=1)

HSP 1 Score: 134.035 bits (336), Expect = 6.249e-32
Identity = 60/110 (54.55%), Postives = 84/110 (76.36%), Query Frame = -2

Query:  301 FSWKSMWRLLSDVFDVEFVELDEKDE-FDLVELMRDKGHVWDMIVEKYGLHKTKLDEIACYEAMVPVLRFEFQHVSSMNKSKDYGFFGYADAFKSIRFWVNKMKEMKLIP 627
            F+WKS+W +L +VFDVEFV  +E DE FD + +++ KG VWD IVEK+GL +TK+++I C+EA+   L F FQHV SMNKS++ GF G+AD  KSI  WV ++++MK+IP
Sbjct:  268 FTWKSLWGVLCEVFDVEFVAFEENDEKFDWLGMIKGKGKVWDEIVEKFGLFETKMEDITCFEALNVGLHFGFQHVCSMNKSRESGFLGFADTLKSIPMWVGRLRDMKIIP 377

HSP 2 Score: 28.4906 bits (62), Expect = 6.249e-32
Identity = 11/20 (55.00%), Postives = 14/20 (70.00%), Query Frame = -3

Query:  603 QNEAFNCTNGDVLVGRVCGG 662
            +N+AFNCTNGDV   +   G
Sbjct:  256 KNQAFNCTNGDVFTWKSLWG 275

[Close]

BLAST of HO064047 vs. TrEMBL
Match: B9MY27_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_782229 PE=4 SV=1)

HSP 1 Score: 132.494 bits (332), Expect = 1.798e-31
Identity = 59/110 (53.64%), Postives = 84/110 (76.36%), Query Frame = -2

Query:  301 FSWKSMWRLLSDVFDVEFVELDEKDE-FDLVELMRDKGHVWDMIVEKYGLHKTKLDEIACYEAMVPVLRFEFQHVSSMNKSKDYGFFGYADAFKSIRFWVNKMKEMKLIP 627
            F+WKS+W +L +VFDVEFV  +E +E FD + +++ KG VWD IVEK+GL +TK+++I C+EA+   L F FQHV SMNKS++ GF G+AD  KSI  WV ++++MK+IP
Sbjct:  268 FTWKSLWGVLCEVFDVEFVAFEENEEKFDWLGMIKGKGKVWDEIVEKFGLFETKMEDITCFEALNVGLHFGFQHVCSMNKSRESGFLGFADTLKSIPMWVGRLRDMKIIP 377

HSP 2 Score: 28.4906 bits (62), Expect = 1.798e-31
Identity = 11/20 (55.00%), Postives = 14/20 (70.00%), Query Frame = -3

Query:  603 QNEAFNCTNGDVLVGRVCGG 662
            +N+AFNCTNGDV   +   G
Sbjct:  256 KNQAFNCTNGDVFTWKSLWG 275

[Close]

BLAST of HO064047 vs. TrEMBL
Match: B9MY28_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_782231 PE=4 SV=1)

HSP 1 Score: 98.5969 bits (244), Expect = 3.020e-21
Identity = 50/110 (45.45%), Postives = 67/110 (60.91%), Query Frame = -2

Query:  301 FSWKSMWRLLSDVFDVEFVELDEKDE-FDLVELMRDKGHVWDMIVEKYGLHKTKLDEIACYEAMVPVLRFEFQHVSSMNKSKDYGFFGYADAFKSIRFWVNKMKEMKLIP 627
            F+WKS+W++L +VFDV FV  +E DE FD + +MR KG +WD IVEK                        FQHV SM KS+++GF GYAD  KSI+ WV +++ MK+IP
Sbjct:   33 FTWKSLWKVLFEVFDVGFVAYEESDEKFDWLGMMRGKGKMWDEIVEK------------------------FQHVCSMIKSREFGFLGYADTLKSIQMWVGRLRAMKMIP 118

HSP 2 Score: 28.1054 bits (61), Expect = 3.020e-21
Identity = 10/12 (83.33%), Postives = 12/12 (100.00%), Query Frame = -3

Query:  627 QNEAFNCTNGDV 662
            +N+AFNCTNGDV
Sbjct:   21 KNQAFNCTNGDV 32

[Close]

BLAST of HO064047 vs. TrEMBL
Match: A9NUS7_PICSI (Putative uncharacterized protein OS=Picea sitchensis PE=2 SV=1)

HSP 1 Score: 92.4337 bits (228), Expect = 6.993e-19
Identity = 43/109 (39.45%), Postives = 70/109 (64.22%), Query Frame = -2

Query:  301 FSWKSMWRLLSDVFDVEFVELDEKDEFDLVELMRDKGHVWDMIVEKYGLHKTKLDEIACYEAMVPVLRFEFQHVSSMNKSKDYGFFGYADAFKSIRFWVNKMKEMKLIP 627
            F WK +W+++++ FD+E +   E + F L E+M+DKG VWD IV +  LH TK++E+  +     VL   +  V SMNKSK+YGFFG+ +   S+  W++K++   ++P
Sbjct:  292 FKWKRLWKIIAEKFDLELLSY-EGEGFSLAEVMKDKGPVWDAIVGENKLHPTKIEEVGNWWFADLVLNPPWGTVLSMNKSKEYGFFGFRNTETSMGQWIDKVRSSNIVP 399

HSP 2 Score: 26.1794 bits (56), Expect = 6.993e-19
Identity = 9/12 (75.00%), Postives = 11/12 (91.67%), Query Frame = -3

Query:  627 QNEAFNCTNGDV 662
            +N+AFNC NGDV
Sbjct:  280 KNQAFNCANGDV 291

[Close]

BLAST of HO064047 vs. TrEMBL
Match: B8LM29_PICSI (Putative uncharacterized protein OS=Picea sitchensis PE=2 SV=1)

HSP 1 Score: 90.8929 bits (224), Expect = 1.538e-18
Identity = 43/109 (39.45%), Postives = 70/109 (64.22%), Query Frame = -2

Query:  301 FSWKSMWRLLSDVFDVEFVELDEKDEFDLVELMRDKGHVWDMIVEKYGLHKTKLDEIACYEAMVPVLRFEFQHVSSMNKSKDYGFFGYADAFKSIRFWVNKMKEMKLIP 627
            F WK +WR++++ FD+E +   E + F L E M+DKG VWD IV +  L+ TK++E+  +     VL   ++ V SMNKSK+YGFFG+ +   S+  W++K++   ++P
Sbjct:  292 FKWKRLWRIIAEKFDLEPLPR-EGEGFSLAEAMKDKGPVWDAIVGENKLYPTKIEEVGNWWFADFVLNVPWEPVFSMNKSKEYGFFGFRNTETSVGQWIDKIRASNIVP 399

HSP 2 Score: 26.5646 bits (57), Expect = 1.538e-18
Identity = 9/12 (75.00%), Postives = 12/12 (100.00%), Query Frame = -3

Query:  627 QNEAFNCTNGDV 662
            +N+AFNC+NGDV
Sbjct:  280 KNQAFNCSNGDV 291

[Close]

BLAST of HO064047 vs. TrEMBL
Match: C0SUC5_TOBAC (Progesterone 5beta reductase-A OS=Nicotiana tabacum GN=Nt-5beta-POR-A PE=2 SV=1)

HSP 1 Score: 93.5893 bits (231), Expect = 1.539e-18
Identity = 43/109 (39.45%), Postives = 74/109 (67.89%), Query Frame = -2

Query:  301 FSWKSMWRLLSDVFDVEFVELDEKDEFDLVELMRDKGHVWDMIVEKYGLHKTKLDEIACYEAMVPVLRFEFQHVSSMNKSKDYGFFGYADAFKSIRFWVNKMKEMKLIP 627
            F WK  W++L++ F VE  E DE++   LVE+M+DKG VWD IV++ GL  TKL+++  +  +  +L  + + + +MNKSK++GF G+ ++ K+   W++K+K  K++P
Sbjct:  282 FKWKHFWKVLAEQFGVEAAEFDEENRCTLVEMMKDKGPVWDEIVKENGLTPTKLEDVGVWWFVDLMLAGDCR-LDTMNKSKEHGFLGFRNSQKAFISWIDKVKAYKVVP 389

HSP 2 Score: 23.8682 bits (50), Expect = 1.539e-18
Identity = 9/12 (75.00%), Postives = 11/12 (91.67%), Query Frame = -3

Query:  627 QNEAFNCTNGDV 662
            +NEAFN +NGDV
Sbjct:  270 KNEAFNVSNGDV 281

[Close]

BLAST of HO064047 vs. TrEMBL
Match: B8LLZ6_PICSI (Putative uncharacterized protein OS=Picea sitchensis PE=2 SV=1)

HSP 1 Score: 87.4261 bits (215), Expect = 5.721e-18
Identity = 43/108 (39.81%), Postives = 66/108 (61.11%), Query Frame = -2

Query:  301 WKSMWRLLSDVFDVEFVELDEKDE-FDLVELMRDKGHVWDMIVEKYGLHKTKLDEIACYEAMVPVLRFEFQHVSSMNKSKDYGFFGYADAFKSIRFWVNKMKEMKLIP 621
            WK +W ++++ FD+E   L  K E F L E M+DKG VWD +V +  LH TK++E+  +      L    + ++SMNKSK+YGFFG+ +   S+  W++KMK    +P
Sbjct:  294 WKRLWGIIAEKFDLE--PLPYKGEGFSLAEAMKDKGPVWDALVRENKLHPTKIEEVGNWWFAEFTLNLPQEMINSMNKSKEYGFFGFRNTETSLGQWIDKMKASNAVP 399

HSP 2 Score: 28.1054 bits (61), Expect = 5.721e-18
Identity = 10/13 (76.92%), Postives = 13/13 (100.00%), Query Frame = -3

Query:  624 QNEAFNCTNGDVL 662
            +N+AFNC+NGDVL
Sbjct:  280 KNQAFNCSNGDVL 292

[Close]

BLAST of HO064047 vs. TrEMBL
Match: A9NUD2_PICSI (Putative uncharacterized protein OS=Picea sitchensis PE=2 SV=1)

HSP 1 Score: 87.4261 bits (215), Expect = 5.721e-18
Identity = 44/108 (40.74%), Postives = 66/108 (61.11%), Query Frame = -2

Query:  301 WKSMWRLLSDVFDVEFVELDEKDE-FDLVELMRDKGHVWDMIVEKYGLHKTKLDEIACYEAMVPVLRFEFQHVSSMNKSKDYGFFGYADAFKSIRFWVNKMKEMKLIP 621
            WK +W ++++ FD+E   L  K E F L E M+DKG VWD +V +  LH TK++E+  +      L    + V SMNKSK+YGFFG+ +   S+  W++KMK   ++P
Sbjct:  294 WKRLWGIIAEKFDLE--PLPYKGEGFSLAEAMKDKGPVWDALVRENKLHPTKIEEVGNWWFADFTLNLPQETVHSMNKSKEYGFFGFRNTETSLGQWIDKMKASNVVP 399

HSP 2 Score: 28.1054 bits (61), Expect = 5.721e-18
Identity = 10/13 (76.92%), Postives = 13/13 (100.00%), Query Frame = -3

Query:  624 QNEAFNCTNGDVL 662
            +N+AFNC+NGDVL
Sbjct:  280 KNQAFNCSNGDVL 292

[Close]

BLAST of HO064047 vs. TrEMBL
Match: D2JSQ9_DIGPU (Progesterone 5beta-reductase 2 OS=Digitalis purpurea GN=P5BR2 PE=2 SV=1)

HSP 1 Score: 92.8189 bits (229), Expect = 7.442e-18
Identity = 47/109 (43.12%), Postives = 68/109 (62.39%), Query Frame = -2

Query:  301 FSWKSMWRLLSDVFDVEFVELDEKDEFDLVELMRDKGHVWDMIVEKYGLHKTKLDEIACYEAMVPVLRFEFQHVSSMNKSKDYGFFGYADAFKSIRFWVNKMKEMKLIP 627
            F WK +W++L+D F++EFV  + K+   L  LM+DK  VWD IVE+Y L  TKL +IA +         E   VSSMNK+K++GF G+ D+ KS    VNK++  + +P
Sbjct:  287 FKWKHIWKVLADQFEIEFVGYEGKEPVSLEGLMKDKDSVWDEIVERYDLVPTKLKDIAAFWFADVAFSIE-GAVSSMNKNKEFGFMGFRDSTKSFISSVNKVRTYRFVP 394

HSP 2 Score: 22.3274 bits (46), Expect = 7.442e-18
Identity = 8/12 (66.67%), Postives = 10/12 (83.33%), Query Frame = -3

Query:  627 QNEAFNCTNGDV 662
            +N+AFN  NGDV
Sbjct:  275 KNQAFNINNGDV 286

[Close]

BLAST of HO064047 vs. TAIR peptide
Match: AT4G24220.1 (| Symbols: VEP1, AWI31 | NAD(P)-binding Rossmann-fold superfamily protein | chr4:12565219-12566474 FORWARD LENGTH=388)

HSP 1 Score: 82.0333 bits (201), Expect = 1.643e-17
Identity = 38/109 (34.86%), Postives = 65/109 (59.63%), Query Frame = -2

Query:  301 FSWKSMWRLLSDVFDVEFVELDEKDEFDLVELMRDKGHVWDMIVEKYGLHKTKLDEIACYEAMVPVLRFEFQHVSSMNKSKDYGFFGYADAFKSIRFWVNKMKEMKLIP 627
            F WK +W++L++ F +E    +E     LVE+M+ K  VW+ +V++  L + KL+E+  +     +L  E   + SMNKSK+YGF G+ ++  S   W++K K  K++P
Sbjct:  281 FKWKHLWKILAEQFGIEEYGFEEGKNLGLVEMMKGKERVWEEMVKENQLQEKKLEEVGVWWFADVILGVEGM-IDSMNKSKEYGFLGFRNSNNSFISWIDKYKAFKIVP 388

HSP 2 Score: 24.6386 bits (52), Expect = 1.643e-17
Identity = 8/12 (66.67%), Postives = 10/12 (83.33%), Query Frame = -3

Query:  627 QNEAFNCTNGDV 662
            +NEAFNC N D+
Sbjct:  269 KNEAFNCNNADI 280

[Close]

BLAST of HO064047 vs. TAIR peptide
Match: AT4G24220.2 (| Symbols: VEP1, AWI31 | NAD(P)-binding Rossmann-fold superfamily protein | chr4:12565219-12566474 FORWARD LENGTH=387)

HSP 1 Score: 82.0333 bits (201), Expect = 1.643e-17
Identity = 38/109 (34.86%), Postives = 65/109 (59.63%), Query Frame = -2

Query:  301 FSWKSMWRLLSDVFDVEFVELDEKDEFDLVELMRDKGHVWDMIVEKYGLHKTKLDEIACYEAMVPVLRFEFQHVSSMNKSKDYGFFGYADAFKSIRFWVNKMKEMKLIP 627
            F WK +W++L++ F +E    +E     LVE+M+ K  VW+ +V++  L + KL+E+  +     +L  E   + SMNKSK+YGF G+ ++  S   W++K K  K++P
Sbjct:  280 FKWKHLWKILAEQFGIEEYGFEEGKNLGLVEMMKGKERVWEEMVKENQLQEKKLEEVGVWWFADVILGVEGM-IDSMNKSKEYGFLGFRNSNNSFISWIDKYKAFKIVP 387

HSP 2 Score: 24.6386 bits (52), Expect = 1.643e-17
Identity = 8/12 (66.67%), Postives = 10/12 (83.33%), Query Frame = -3

Query:  627 QNEAFNCTNGDV 662
            +NEAFNC N D+
Sbjct:  268 KNEAFNCNNADI 279

[Close]

BLAST of HO064047 vs. TAIR peptide
Match: AT5G58750.1 (| Symbols: | NAD(P)-binding Rossmann-fold superfamily protein | chr5:23728886-23730046 FORWARD LENGTH=386)

HSP 1 Score: 55.8398 bits (133), Expect = 2.399e-8
Identity = 32/110 (29.09%), Postives = 56/110 (50.91%), Query Frame = -2

Query:  301 FSWKSMWRLLSDVFDVEFVELDEKDE-FDLVELMRDKGHVWDMIVEKYGLHKTKLDEIACYEAMVPVLRFEFQHVSSMNKSKDYGFFGYADAFKSIRFWVNKMKEMKLIP 627
            F+WK +W  +     V+  E    DE F     M ++ HVWD IV K  L +T+++++A +  +  + R  F+ +    K   +GF        S+ +W++ M++ KLIP
Sbjct:  276 FTWKEIWPEIGKKLGVQVNETTMFDEGFWFGREMVERKHVWDEIVVKEKLVRTEIEDLANWYFLDALFRCPFKLLGKREKVDRFGFKRKYRTLDSVLYWIDVMRDEKLIP 385

The following BLAST results are available for this feature:

BLAST of HO064047 vs. TrEMBL
BLAST of HO064047 vs. TAIR peptide

BLAST of HO064047 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
B9MY26_POPTR	2.014e-33	57.27	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
A9PJM4_9ROSI	6.249e-32	54.55	Putative uncharacterized protein OS=Populus tricho...	[more]
B9MY27_POPTR	1.798e-31	53.64	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
B9MY28_POPTR	3.020e-21	45.45	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
A9NUS7_PICSI	6.993e-19	39.45	Putative uncharacterized protein OS=Picea sitchens...	[more]
B8LM29_PICSI	1.538e-18	39.45	Putative uncharacterized protein OS=Picea sitchens...	[more]
C0SUC5_TOBAC	1.539e-18	39.45	Progesterone 5beta reductase-A OS=Nicotiana tabacu...	[more]
B8LLZ6_PICSI	5.721e-18	39.81	Putative uncharacterized protein OS=Picea sitchens...	[more]
A9NUD2_PICSI	5.721e-18	40.74	Putative uncharacterized protein OS=Picea sitchens...	[more]
D2JSQ9_DIGPU	7.442e-18	43.12	Progesterone 5beta-reductase 2 OS=Digitalis purpur...	[more]

BLAST of HO064047 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide)
Total hits: 3


Match Name	E-value	Identity	Description
AT4G24220.1	1.643e-17	34.86	\| Symbols: VEP1, AWI31 \| NAD(P)-binding Rossmann-f...	[more]
AT4G24220.2	1.643e-17	34.86	\| Symbols: VEP1, AWI31 \| NAD(P)-binding Rossmann-f...	[more]
AT5G58750.1	2.399e-8	29.09	\| Symbols: \| NAD(P)-binding Rossmann-fold superfa...	[more]

InterPro

Analysis Name: InterProScan analysis for Cicer arietinum unigene v1
Date Performed: 2010-12-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	SEG	seg	seg	coord: 34..53 score

Properties


Property Name	Value
Singlet in analysis	Cicer arietinum unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>HO064047 ID=HO064047; Name=HO064047; organism=Cicer arietinum; type=EST; length=670bp
ACTCACATAAATCGCTAAGCCTGTATATTCATAAGCCAATACAAAGAGAA
GAAGTTAGTTACAACTAGTTTAACATAAAGAAACTAACTTCCAGCTTGGT
TGAGTAATAAGCAAACTCCACCAAGAGAACATTAATCCCATTTTATTCGT
ACCATAGCTCCGCAAACAATATTATTAATTAATCCAACATCACTCTTTTA
CATTACAAATACCAACAGAACATAAAAAAGCAAACAATATTATTAATTAA
TCCAACACAATAAAATCACATTATGCATTTGTAGGTCAATGTTGGTAGGT
GGGTATGAGTTTCATTTCCTTCATCTTGTTCACCCAGAATCTGATACTCT
TAAACGCATCAGCGTATCCAAAAAAACCATAATCCTTACTCTTATTCATA
CTAGATACATGTTGAAACTCAAACCGTAGAACAGGCACCATAGCTTCATA
GCAAGCAATTTCATCCAATTTAGTTTTGTGAAGACCATATTTCTCCACAA
TCATATCCCAAACGTGACCTTTATCACGCATCAACTCAACCAAATCAAAC
TCATCCTTCTCATCCAACTCCACAAACTCAACATCAAATACATCGCTCAA
CAACCTCCACATACTCTTCCAACTAAAACGTCGCCGTTTGTGCAATTAAA
CGCTTCGTTTTGGCATGCTC

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