HO067811

EST Overview


Name	HO067811
Unique Name	HO067811
Type	EST
Organism	Cicer arietinum (chickpea)
Sequence length	590

Libraries


Library Name	Type
LIBEST_026415 Chickpea drought stressed cDNA SSH library BULK2	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Cicer arietinum unigene v1	Cicer arietinum unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Cicer arietinum unigene v1	2010-12-27
Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide	2011-02-03
KEGG analysis for Cicer arietinum unigene v1	2011-01-05
Homology Analysis: Cicer arietinum unigene v1 vs Swissprot	2010-12-28
Homology Analysis: Cicer arietinum unigene v1 vs Trembl	2010-12-28
InterProScan analysis for Cicer arietinum unigene v1	2010-12-29

Homology

[Close]

BLAST of HO067811 vs. SwissProt
Match: AASS_ARATH (Alpha-aminoadipic semialdehyde synthase OS=Arabidopsis thaliana GN=LKR/SDH PE=1 SV=1)

HSP 1 Score: 202.601 bits (514), Expect = 1.217e-51
Identity = 99/161 (61.49%), Postives = 129/161 (80.12%), Query Frame = -2

Query:  104 LMGEEDITEKILTLGHCKDQGAAMMTAKTIIFLGLLDQTEIPASCRSAFDVACFCMGERLSYSSTEKDMVLLHHEVEIEYPDSQITEKHRATLLEFGKTVDGKTTTAMALTVGIPAAVGALLLLTNKIQTRGVLRPIQPEVYTPALDIIQAYGIKLIEKNE 586
            L GEE+I+++I+ LGH K+  A    AKTI+FLG  ++ E+P+ C+S FD  C+ M E+L+YS  E+DMVLLHHEVE+E+ +S+  EKH ATLLEFG   +G+TTTAMA TVGIPAA+GALLL+ +KI+TRGVLRP++ EVY PALDI+QAYGIKL+EK E
Sbjct:  906 LAGEEEISKRIIKLGHSKETAAK--AAKTIVFLGFNEEREVPSLCKSVFDATCYLMEEKLAYSGNEQDMVLLHHEVEVEFLESKRIEKHTATLLEFGDIKNGQTTTAMAKTVGIPAAIGALLLIEDKIKTRGVLRPLEAEVYLPALDILQAYGIKLMEKAE 1064

[Close]

BLAST of HO067811 vs. SwissProt
Match: SCPDH_DICDI (Probable saccharopine dehydrogenase [NADP+, L-glutamate-forming] OS=Dictyostelium discoideum GN=sdh PE=2 SV=1)

HSP 1 Score: 78.5666 bits (192), Expect = 2.651e-14
Identity = 43/130 (33.08%), Postives = 74/130 (56.92%), Query Frame = -2

Query:  104 FLGLLDQTEIPASCRSAFDVACFCMGERLSYSSTEKDMVLLHHEVEIEYPDSQITEKHRATLLEFGKTVDGKTTTAMALTVGIPAAVGALLLLTNKIQTRGVLRPIQPEVYTPALDIIQAYGIKLIEKNE 493
            +LGLL   E   +  +  D  C  + ++LSY + E+D+V+L H   ++Y D   TEK  ++L+ +G       ++A +LTVG+P  +   L+   K  TRGV+ P+ PE Y P L+ +++  I++IE  +
Sbjct:  352 WLGLLSADEKVVNKNTPIDSLCALLEKKLSYKAGERDVVVLEHNFVVQYADR--TEKEVSSLICYGIP---NGSSATSLTVGVPVGIATELIADGKTTTRGVVGPVTPEFYLPILEKLKSENIEMIETKQ 476

[Close]

BLAST of HO067811 vs. SwissProt
Match: LYS9_YEAST (Saccharopine dehydrogenase [NADP+, L-glutamate-forming] OS=Saccharomyces cerevisiae GN=LYS9 PE=1 SV=1)

HSP 1 Score: 72.7886 bits (177), Expect = 1.455e-12
Identity = 52/157 (33.12%), Postives = 78/157 (49.68%), Query Frame = -2

Query:  110 EEDITEKILTLGHCKDQGAAMMTAKTIIFLGLLDQTEIPASCRSAFDVACFCMGERLSYSSTEKDMVLLHHEVEIEYPDSQITEKHRATLLEFGKTVDGKTTTAMALTVGIPAAVGALLLLTNKIQTRGVLRPIQPEVYTPAL-DIIQAYGIKLIEK 577
            +ED+   I +    KD            +LGL    +I     +A D  C  + E + Y   E+DMV+L H+  IE+ D   TE   +TL+++GK V G ++  MA TVG P A+    +L   I+  G+L P  PE+  P + ++   YGI L EK
Sbjct:  292 KEDLIASIDSKATWKDDEDRERILSGFAWLGLFSDAKITPR-GNALDTLCARLEELMQYEDNERDMVVLQHKFGIEWADGT-TETRTSTLVDYGK-VGGYSS--MAATVGYPVAIATKFVLDGTIKGPGLLAPYSPEINDPIMKELKDKYGIYLKEK 443

[Close]

BLAST of HO067811 vs. SwissProt
Match: LYS9_MAGGR (Saccharopine dehydrogenase [NADP+, L-glutamate-forming] OS=Magnaporthe grisea GN=LYS3 PE=1 SV=2)

HSP 1 Score: 71.633 bits (174), Expect = 3.240e-12
Identity = 45/157 (28.66%), Postives = 77/157 (49.04%), Query Frame = -2

Query:  110 EEDITEKILTLGHCKDQGAAMMTAKTIIFLGLLDQTEIPASCRSAFDVACFCMGERLSYSSTEKDMVLLHHEVEIEYPDSQITEKHRATLLEFGKTVDGKTTTAMALTVGIPAAVGALLLLTNKIQTRGVLRPIQPEVYTPAL-DIIQAYGIKLIEK 577
            E+DI   I++    +           + +LG+    +I     +A D  C  + E++ +   E+D+V+L H+ EIE  D    E   ++L E+G  +     +AMA  VG+P AV    +L   I  RGVL P+  ++  P + ++ + YGI+  EK
Sbjct:  293 EQDIVSTIVSNATFESTEEQKRIVAGLKWLGIFSDKKITPR-GNALDTLCATLEEKMQFEEGERDLVMLQHKFEIENKDGS-RETRTSSLCEYGAPIGSGGYSAMAKLVGVPCAVAVKFVLDGTISDRGVLAPMNSKINDPLMKELKEKYGIECKEK 447

[Close]

BLAST of HO067811 vs. SwissProt
Match: AASS_BOVIN (Alpha-aminoadipic semialdehyde synthase, mitochondrial OS=Bos taurus GN=AASS PE=2 SV=1)

HSP 1 Score: 71.2478 bits (173), Expect = 4.232e-12
Identity = 45/137 (32.85%), Postives = 71/137 (51.82%), Query Frame = -2

Query:  125 KDQGAAMMTAKTIIFLGLLDQTEIPASCRSAFDVACFCMGERLSYSSTEKDMVLLHHEVEIEYPDSQITEKHRATLLEFGKTVDGKTTTAMALTVGIPAAVGALLLLTNKIQTRGVLRPIQPEVYTPALDIIQAYGI 535
            K  G      + + +LGLL   ++P +  S  D     +  +LSY   EKDM+++     I +P   +  K    L+ +G   D    +AMA TVG+P A+ A +LL  +IQ +G++ P   E+Y P L+ I+A GI
Sbjct:  785 KKLGGDTTQLEALEWLGLLGDEQVPQA-ESLVDALSKHLAVKLSYGPGEKDMIVMRDSFGIRHPSGHLENK-TIDLVVYG---DVNGFSAMAKTVGLPTAMAAKMLLDGEIQAKGLMGPFSKEIYGPILERIKAEGI 916

[Close]

BLAST of HO067811 vs. SwissProt
Match: AASS_RAT (Alpha-aminoadipic semialdehyde synthase, mitochondrial OS=Rattus norvegicus GN=Aass PE=2 SV=1)

HSP 1 Score: 70.8626 bits (172), Expect = 5.527e-12
Identity = 43/123 (34.96%), Postives = 68/123 (55.28%), Query Frame = -2

Query:  125 FLGLLDQTEIPASCRSAFDVACFCMGERLSYSSTEKDMVLLHHEVEIEYPDSQITEKHRATLLEFGKTVDGKTTTAMALTVGIPAAVGALLLLTNKIQTRGVLRPIQPEVYTPALDIIQAYGI 493
            +LGLL   ++P +  S  D     +  +LSY   EKDM+++     I +P   +  K    L+ +G   D    +AMA TVG+P A+ A +LL  +I+T+G++ P   E+Y P L+ I+A GI
Sbjct:  799 WLGLLGDEQVPQA-ESIVDAFSKHLVSKLSYGPEEKDMIVMRDSFGIRHPSGHLENK-TIDLVVYG---DFNGFSAMAKTVGLPTAMAAKMLLDGEIETKGLMGPFSKEIYGPILERIKAEGI 916

[Close]

BLAST of HO067811 vs. SwissProt
Match: AASS_MOUSE (Alpha-aminoadipic semialdehyde synthase, mitochondrial OS=Mus musculus GN=Aass PE=2 SV=1)

HSP 1 Score: 68.5514 bits (166), Expect = 2.743e-11
Identity = 42/123 (34.15%), Postives = 67/123 (54.47%), Query Frame = -2

Query:  125 FLGLLDQTEIPASCRSAFDVACFCMGERLSYSSTEKDMVLLHHEVEIEYPDSQITEKHRATLLEFGKTVDGKTTTAMALTVGIPAAVGALLLLTNKIQTRGVLRPIQPEVYTPALDIIQAYGI 493
            +LGLL   ++P +  S  D     +  +LSY   EKDM+++     I +P   +  K    L+ +G   D    +AMA TVG+P A+ A +LL  +I+ +G++ P   E+Y P L+ I+A GI
Sbjct:  799 WLGLLGDEQVPQA-ESIVDAFSKHLVSKLSYGPEEKDMIVMRDSFGIRHPSGHLENK-TIDLVVYG---DFNGFSAMAKTVGLPTAMAAKMLLDGEIEAKGLMGPFTKEIYGPILERIKAEGI 916

[Close]

BLAST of HO067811 vs. SwissProt
Match: AASS_HUMAN (Alpha-aminoadipic semialdehyde synthase, mitochondrial OS=Homo sapiens GN=AASS PE=1 SV=1)

HSP 1 Score: 67.3958 bits (163), Expect = 6.111e-11
Identity = 42/123 (34.15%), Postives = 66/123 (53.66%), Query Frame = -2

Query:  125 FLGLLDQTEIPASCRSAFDVACFCMGERLSYSSTEKDMVLLHHEVEIEYPDSQITEKHRATLLEFGKTVDGKTTTAMALTVGIPAAVGALLLLTNKIQTRGVLRPIQPEVYTPALDIIQAYGI 493
            +LGLL   ++P +  S  D     +  +LSY   EKDM+++     I +P   +  K    L+ +G   D    +AMA TVG+P A+ A +LL  +I  +G++ P   E+Y P L+ I+A GI
Sbjct:  799 WLGLLGDEQVPQA-ESILDALSKHLVMKLSYGPEEKDMIVMRDSFGIRHPSGHLEHK-TIDLVAYG---DINGFSAMAKTVGLPTAMAAKMLLDGEIGAKGLMGPFSKEIYGPILERIKAEGI 916

[Close]

BLAST of HO067811 vs. SwissProt
Match: LYS9_SCHPO (Saccharopine dehydrogenase [NADP+, L-glutamate-forming] OS=Schizosaccharomyces pombe GN=lys9 PE=2 SV=1)

HSP 1 Score: 65.4698 bits (158), Expect = 2.322e-10
Identity = 43/156 (27.56%), Postives = 74/156 (47.44%), Query Frame = -2

Query:  110 EEDITEKILTLGHCKDQGAAMMTAKTIIFLGLLDQTEIPASCRSAFDVACFCMGERLSYSSTEKDMVLLHHEVEIEYPDSQITEKHRATLLEFGKTVDGKTTTAMALTVGIPAAVGALLLLTNKIQTRGVLRPIQPEVYTPALDIIQAYGIKLIEK 577
            E D+ +KI ++   KD+         + +LG+     +     +  D  C  + E + Y   E+DM++L H+ E+E  + +  +    TLL++G        T+MA  VG+P  V    +L   I T GVL P   ++  P +D +   GI+L E+
Sbjct:  295 EADLIKKISSIHKFKDEDDKKRILNGLKWLGMFSSKPVTPR-GNPLDTLCATLEELMQYEEGERDMLILQHKFEVETKEGK-RQTRTCTLLDYGVP---NGYTSMAKLVGVPCGVATQQILDGVINTPGVLAPNDMKLCGPLIDTLAKEGIRLEEE 445

[Close]

BLAST of HO067811 vs. TrEMBL
Match: B9MT60_POPTR (Predicted protein (Fragment) OS=Populus trichocarpa GN=POPTRDRAFT_739274 PE=4 SV=1)

HSP 1 Score: 245.358 bits (625), Expect = 2.261e-63
Identity = 117/160 (73.12%), Postives = 141/160 (88.12%), Query Frame = -2

Query:  104 MGEEDITEKILTLGHCKDQGAAMMTAKTIIFLGLLDQTEIPASCRSAFDVACFCMGERLSYSSTEKDMVLLHHEVEIEYPDSQITEKHRATLLEFGKTVDGKTTTAMALTVGIPAAVGALLLLTNKIQTRGVLRPIQPEVYTPALDIIQAYGIKLIEKNE 583
            +GE+ I+E+I+ LGHCK+QG A+ TAKTII+LGL +QTEIP SC+SAFDV C+ M ERL+YSSTE+DMVLLHHE+E+E+PDSQ TE H+ TLLEFG+T +GKTTTAMALTVGIP A+GALLLL NKI TRGVLRP +PEVY PALDI+QAYGIK++EK E
Sbjct:  840 LGEKHISERIVALGHCKEQGTAVRTAKTIIYLGLHEQTEIPVSCQSAFDVTCYRMEERLAYSSTEQDMVLLHHEMEVEFPDSQATENHKGTLLEFGRTGNGKTTTAMALTVGIPVAIGALLLLENKINTRGVLRPFEPEVYVPALDILQAYGIKVMEKVE 999

[Close]

BLAST of HO067811 vs. TrEMBL
Match: B9RR16_RICCO (Aminoadipic semialdehyde synthase, putative OS=Ricinus communis GN=RCOM_0708300 PE=4 SV=1)

HSP 1 Score: 243.047 bits (619), Expect = 1.122e-62
Identity = 119/161 (73.91%), Postives = 140/161 (86.96%), Query Frame = -2

Query:  104 LMGEEDITEKILTLGHCKDQGAAMMTAKTIIFLGLLDQTEIPASCRSAFDVACFCMGERLSYSSTEKDMVLLHHEVEIEYPDSQITEKHRATLLEFGKTVDGKTTTAMALTVGIPAAVGALLLLTNKIQTRGVLRPIQPEVYTPALDIIQAYGIKLIEKNE 586
            L+GEEDITEK++TLGHCK++  A+  AKTII+LGL +QTEIPASC+S FDV CF M ERL+YSS E+DMVLLHHEVE+E+PD + TE HR TLLEFG T  GKT TAMALTVGIPAA+GALLLL NKI+T+GV+RPI+PEVY PALDI+QA+GIKLIEK E
Sbjct:  890 LLGEEDITEKLVTLGHCKEKETAVKAAKTIIYLGLHEQTEIPASCKSPFDVTCFRMEERLTYSSAEQDMVLLHHEVEVEFPDGKRTEYHRGTLLEFGTTKKGKTITAMALTVGIPAAIGALLLLENKIKTKGVVRPIEPEVYVPALDILQAHGIKLIEKVE 1050

[Close]

BLAST of HO067811 vs. TrEMBL
Match: D7TC82_VITVI (Whole genome shotgun sequence of line PN40024, scaffold_16.assembly12x (Fragment) OS=Vitis vinifera GN=VIT_00014998001 PE=4 SV=1)

HSP 1 Score: 234.958 bits (598), Expect = 3.056e-60
Identity = 116/160 (72.50%), Postives = 135/160 (84.38%), Query Frame = -2

Query:  104 MGEEDITEKILTLGHCKDQGAAMMTAKTIIFLGLLDQTEIPASCRSAFDVACFCMGERLSYSSTEKDMVLLHHEVEIEYPDSQITEKHRATLLEFGKTVDGKTTTAMALTVGIPAAVGALLLLTNKIQTRGVLRPIQPEVYTPALDIIQAYGIKLIEKNE 583
            M  EDI E+IL LG CK Q  A+ TAKTI++LG  +QTEIP SCRSAFDVAC  M ERL+YSS E+DMVLLHHEVE+E+PD +  EKHRATLLEFGKT +GKTTTAMA TVGIPAA+GALL+L  KI+TRGVLRPI+P+VY PALDI+QAYG+KL+EK E
Sbjct:  893 MTAEDIKERILALGLCKVQVTALKTAKTILYLGFHEQTEIPVSCRSAFDVACLRMEERLAYSSEEQDMVLLHHEVEVEFPDGRPVEKHRATLLEFGKTKNGKTTTAMAFTVGIPAAIGALLILEKKIKTRGVLRPIEPQVYVPALDILQAYGLKLLEKTE 1052

[Close]

BLAST of HO067811 vs. TrEMBL
Match: Q8H770_GOSHI (Bifunctional lysine-ketoglutarate reductase/saccharopine dehydrogenase OS=Gossypium hirsutum PE=2 SV=1)

HSP 1 Score: 222.246 bits (565), Expect = 2.050e-56
Identity = 108/161 (67.08%), Postives = 133/161 (82.61%), Query Frame = -2

Query:  104 LMGEEDITEKILTLGHCKDQGAAMMTAKTIIFLGLLDQTEIPASCRSAFDVACFCMGERLSYSSTEKDMVLLHHEVEIEYPDSQITEKHRATLLEFGKTVDGKTTTAMALTVGIPAAVGALLLLTNKIQTRGVLRPIQPEVYTPALDIIQAYGIKLIEKNE 586
            ++GE+ I E+IL LGHCK+ G A+  AKTI+FLGL +QT IP SC+SAF V C  M ERL+YS+TE+DMVLLHHEVE+++PDS+ TE+H ATLLEFGK  +GK  +AMALTVG+P A+GALLL+ NKI+TRGVL PI PEVY PAL+I QAYGIKL+EK E
Sbjct:  892 VVGEKKIAERILELGHCKEPGVAVKAAKTIVFLGLNEQTGIPVSCQSAFAVTCHRMEERLTYSNTEQDMVLLHHEVEVDFPDSKQTERHTATLLEFGKAKNGKMISAMALTVGVPVAIGALLLIVNKIKTRGVLGPIVPEVYLPALEIAQAYGIKLMEKTE 1052

[Close]

BLAST of HO067811 vs. TrEMBL
Match: D7M991_ARALY (Lysine-ketoglutarate reductase/saccharopine dehydrogenase bifunctional enzyme OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_491371 PE=4 SV=1)

HSP 1 Score: 208.379 bits (529), Expect = 3.063e-52
Identity = 101/161 (62.73%), Postives = 131/161 (81.37%), Query Frame = -2

Query:  104 LMGEEDITEKILTLGHCKDQGAAMMTAKTIIFLGLLDQTEIPASCRSAFDVACFCMGERLSYSSTEKDMVLLHHEVEIEYPDSQITEKHRATLLEFGKTVDGKTTTAMALTVGIPAAVGALLLLTNKIQTRGVLRPIQPEVYTPALDIIQAYGIKLIEKNE 586
            L GEE+I+++I  LG+ K+  A    AKTI+FLG  ++ EIP+ C+S FD  C+ M E+L+YS  E+DMVLLHHEVE+E+P+S+  EKH ATLLEFG+  +G+TTTAMA TVGIPAA+GALLL+ +KI+TRGVLRP++PEVY PALDI+QAYGIKL+EK E
Sbjct:  897 LAGEEEISKRIFKLGYSKETAAK--AAKTIVFLGFNEEREIPSLCKSVFDATCYLMEEKLAYSGNEQDMVLLHHEVEVEFPESKRIEKHTATLLEFGEIKNGQTTTAMAKTVGIPAAIGALLLIEDKIKTRGVLRPLEPEVYLPALDILQAYGIKLMEKTE 1055

[Close]

BLAST of HO067811 vs. TrEMBL
Match: Q1KL68_9ORYZ (Lysine ketoglutarate reductase/saccharopine dehydrogenase biofunctional enzyme OS=Zizania latifolia GN=LKR/SDH PE=2 SV=1)

HSP 1 Score: 207.994 bits (528), Expect = 4.000e-52
Identity = 98/158 (62.03%), Postives = 129/158 (81.65%), Query Frame = -2

Query:  104 EEDITEKILTLGHCKDQGAAMMTAKTIIFLGLLDQTEIPASCRSAFDVACFCMGERLSYSSTEKDMVLLHHEVEIEYPDSQITEKHRATLLEFGKTVDGKTTTAMALTVGIPAAVGALLLLTNKIQTRGVLRPIQPEVYTPALDIIQAYGIKLIEKNE 577
            E ++  ++L+ GHCK++  A+ T KTI FLGL ++TEIP +C SAFDV C  M +RL+Y   E+DMVLLHHEVE+EYPD + TEKH+ATLLEFGK  +G++TTAMALTVGIPAA+GALLLL NKIQ +GV+RP++PE+Y PAL+I+++ GIKL E+ E
Sbjct:  816 EGELISRLLSFGHCKEKEIAVKTVKTIKFLGLYEETEIPKNCSSAFDVICQRMEQRLAYGHNEQDMVLLHHEVEVEYPDGRPTEKHQATLLEFGKVENGRSTTAMALTVGIPAAIGALLLLQNKIQRKGVIRPLEPEIYIPALEILESSGIKLAERVE 973

[Close]

BLAST of HO067811 vs. TrEMBL
Match: Q8GTD2_HORVD (Saccharopin dehydrogenase-like protein OS=Hordeum vulgare var. distichum GN=sdh PE=2 SV=1)

HSP 1 Score: 206.838 bits (525), Expect = 8.912e-52
Identity = 96/158 (60.76%), Postives = 129/158 (81.65%), Query Frame = -2

Query:  104 EEDITEKILTLGHCKDQGAAMMTAKTIIFLGLLDQTEIPASCRSAFDVACFCMGERLSYSSTEKDMVLLHHEVEIEYPDSQITEKHRATLLEFGKTVDGKTTTAMALTVGIPAAVGALLLLTNKIQTRGVLRPIQPEVYTPALDIIQAYGIKLIEKNE 577
            ++++  +++ LGHCK++  A+   KTI FLGL ++TEIP  C SAF V C  M +R++Y   E+DMVLLHHEVE+EYPD + TEKH+ATLLEFGKT +G++TTAMALTVG+PAA+GALLLL NK+Q +GV+RP+QPE+Y PAL+I++A GIKLIE+ E
Sbjct:  362 DDELISRLMMLGHCKEKELAVKILKTIKFLGLHEETEIPKDCSSAFSVICQRMEQRMAYGHNEQDMVLLHHEVEVEYPDGRPTEKHQATLLEFGKTENGRSTTAMALTVGVPAAIGALLLLQNKVQRKGVIRPLQPEIYIPALEILEASGIKLIERVE 519

[Close]

BLAST of HO067811 vs. TrEMBL
Match: O04709_MAIZE (Lysine ketoglutarate reductase/saccharopine dehydrogenase OS=Zea mays GN=LKRSDH PE=2 SV=3)

HSP 1 Score: 206.453 bits (524), Expect = 1.164e-51
Identity = 97/158 (61.39%), Postives = 128/158 (81.01%), Query Frame = -2

Query:  104 EEDITEKILTLGHCKDQGAAMMTAKTIIFLGLLDQTEIPASCRSAFDVACFCMGERLSYSSTEKDMVLLHHEVEIEYPDSQITEKHRATLLEFGKTVDGKTTTAMALTVGIPAAVGALLLLTNKIQTRGVLRPIQPEVYTPALDIIQAYGIKLIEKNE 577
            ++D+  ++L LG CK++  A+ T KTI FLGL ++T+IP  C S FDV C  M +R++Y   E+DMVLLHHEVE+EYPD Q  EKH+ATLLEFGK  +G++TTAMALTVGIPAA+GALLLL NK+QT+GV+RP+QPE+Y PAL+I+++ GIKL+EK E
Sbjct:  902 DDDLIARLLKLGCCKNKEIAVKTVKTIKFLGLHEETQIPKGCSSPFDVICQRMEQRMAYGHNEQDMVLLHHEVEVEYPDGQPAEKHQATLLEFGKVENGRSTTAMALTVGIPAAIGALLLLKNKVQTKGVIRPLQPEIYVPALEILESSGIKLVEKVE 1059

[Close]

BLAST of HO067811 vs. TrEMBL
Match: Q6K7D6_ORYSJ (Putative lysine-ketoglutarate reductase/saccharopine dehydrogenase bifunctional enzyme OS=Oryza sativa subsp. japonica GN=OJ1311_D08.27 PE=4 SV=1)

HSP 1 Score: 204.142 bits (518), Expect = 5.776e-51
Identity = 96/156 (61.54%), Postives = 126/156 (80.77%), Query Frame = -2

Query:  104 DITEKILTLGHCKDQGAAMMTAKTIIFLGLLDQTEIPASCRSAFDVACFCMGERLSYSSTEKDMVLLHHEVEIEYPDSQITEKHRATLLEFGKTVDGKTTTAMALTVGIPAAVGALLLLTNKIQTRGVLRPIQPEVYTPALDIIQAYGIKLIEKNE 571
            ++  ++L+ GHCKD+  A  T KTI FLGL ++T+IP +C SAFDV C  M +R++Y   E+DMVLLHHEVE+EYPD + TEKH+ATLLEFGK  +G+ TTAMALTVGIPAA+GALLLL NKIQ +GV+RP++PE+Y PAL+I+++ GIKL E+ E
Sbjct:  377 ELISRLLSFGHCKDKEIAAKTVKTIKFLGLYEETQIPENCSSAFDVICQRMEQRMAYIHNEQDMVLLHHEVEVEYPDGRPTEKHQATLLEFGKVENGRPTTAMALTVGIPAAIGALLLLQNKIQKKGVIRPLEPEIYIPALEILESSGIKLAERVE 532

[Close]

BLAST of HO067811 vs. TrEMBL
Match: B9F3L6_ORYSJ (Putative uncharacterized protein OS=Oryza sativa subsp. japonica GN=OsJ_08631 PE=4 SV=1)

HSP 1 Score: 204.142 bits (518), Expect = 5.776e-51
Identity = 96/156 (61.54%), Postives = 126/156 (80.77%), Query Frame = -2

Query:  104 DITEKILTLGHCKDQGAAMMTAKTIIFLGLLDQTEIPASCRSAFDVACFCMGERLSYSSTEKDMVLLHHEVEIEYPDSQITEKHRATLLEFGKTVDGKTTTAMALTVGIPAAVGALLLLTNKIQTRGVLRPIQPEVYTPALDIIQAYGIKLIEKNE 571
            ++  ++L+ GHCKD+  A  T KTI FLGL ++T+IP +C SAFDV C  M +R++Y   E+DMVLLHHEVE+EYPD + TEKH+ATLLEFGK  +G+ TTAMALTVGIPAA+GALLLL NKIQ +GV+RP++PE+Y PAL+I+++ GIKL E+ E
Sbjct:  804 ELISRLLSFGHCKDKEIAAKTVKTIKFLGLYEETQIPENCSSAFDVICQRMEQRMAYIHNEQDMVLLHHEVEVEYPDGRPTEKHQATLLEFGKVENGRPTTAMALTVGIPAAIGALLLLQNKIQKKGVIRPLEPEIYIPALEILESSGIKLAERVE 959

[Close]

BLAST of HO067811 vs. TAIR peptide
Match: AT4G33150.3 (| Symbols: | lysine-ketoglutarate reductase/saccharopine dehydrogenase bifunctional enzyme | chr4:15985479-15988055 REVERSE LENGTH=482)

HSP 1 Score: 202.601 bits (514), Expect = 1.280e-52
Identity = 99/161 (61.49%), Postives = 129/161 (80.12%), Query Frame = -2

Query:  104 LMGEEDITEKILTLGHCKDQGAAMMTAKTIIFLGLLDQTEIPASCRSAFDVACFCMGERLSYSSTEKDMVLLHHEVEIEYPDSQITEKHRATLLEFGKTVDGKTTTAMALTVGIPAAVGALLLLTNKIQTRGVLRPIQPEVYTPALDIIQAYGIKLIEKNE 586
            L GEE+I+++I+ LGH K+  A    AKTI+FLG  ++ E+P+ C+S FD  C+ M E+L+YS  E+DMVLLHHEVE+E+ +S+  EKH ATLLEFG   +G+TTTAMA TVGIPAA+GALLL+ +KI+TRGVLRP++ EVY PALDI+QAYGIKL+EK E
Sbjct:  324 LAGEEEISKRIIKLGHSKETAAK--AAKTIVFLGFNEEREVPSLCKSVFDATCYLMEEKLAYSGNEQDMVLLHHEVEVEFLESKRIEKHTATLLEFGDIKNGQTTTAMAKTVGIPAAIGALLLIEDKIKTRGVLRPLEAEVYLPALDILQAYGIKLMEKAE 482

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BLAST of HO067811 vs. TAIR peptide
Match: AT4G33150.2 (| Symbols: | lysine-ketoglutarate reductase/saccharopine dehydrogenase bifunctional enzyme | chr4:15985479-15991069 REVERSE LENGTH=1064)

HSP 1 Score: 202.601 bits (514), Expect = 1.280e-52
Identity = 99/161 (61.49%), Postives = 129/161 (80.12%), Query Frame = -2

Query:  104 LMGEEDITEKILTLGHCKDQGAAMMTAKTIIFLGLLDQTEIPASCRSAFDVACFCMGERLSYSSTEKDMVLLHHEVEIEYPDSQITEKHRATLLEFGKTVDGKTTTAMALTVGIPAAVGALLLLTNKIQTRGVLRPIQPEVYTPALDIIQAYGIKLIEKNE 586
            L GEE+I+++I+ LGH K+  A    AKTI+FLG  ++ E+P+ C+S FD  C+ M E+L+YS  E+DMVLLHHEVE+E+ +S+  EKH ATLLEFG   +G+TTTAMA TVGIPAA+GALLL+ +KI+TRGVLRP++ EVY PALDI+QAYGIKL+EK E
Sbjct:  906 LAGEEEISKRIIKLGHSKETAAK--AAKTIVFLGFNEEREVPSLCKSVFDATCYLMEEKLAYSGNEQDMVLLHHEVEVEFLESKRIEKHTATLLEFGDIKNGQTTTAMAKTVGIPAAIGALLLIEDKIKTRGVLRPLEAEVYLPALDILQAYGIKLMEKAE 1064

[Close]

BLAST of HO067811 vs. TAIR peptide
Match: AT4G33150.1 (| Symbols: | lysine-ketoglutarate reductase/saccharopine dehydrogenase bifunctional enzyme | chr4:15985479-15991069 REVERSE LENGTH=1064)

HSP 1 Score: 202.601 bits (514), Expect = 1.280e-52
Identity = 99/161 (61.49%), Postives = 129/161 (80.12%), Query Frame = -2

Query:  104 LMGEEDITEKILTLGHCKDQGAAMMTAKTIIFLGLLDQTEIPASCRSAFDVACFCMGERLSYSSTEKDMVLLHHEVEIEYPDSQITEKHRATLLEFGKTVDGKTTTAMALTVGIPAAVGALLLLTNKIQTRGVLRPIQPEVYTPALDIIQAYGIKLIEKNE 586
            L GEE+I+++I+ LGH K+  A    AKTI+FLG  ++ E+P+ C+S FD  C+ M E+L+YS  E+DMVLLHHEVE+E+ +S+  EKH ATLLEFG   +G+TTTAMA TVGIPAA+GALLL+ +KI+TRGVLRP++ EVY PALDI+QAYGIKL+EK E
Sbjct:  906 LAGEEEISKRIIKLGHSKETAAK--AAKTIVFLGFNEEREVPSLCKSVFDATCYLMEEKLAYSGNEQDMVLLHHEVEVEFLESKRIEKHTATLLEFGDIKNGQTTTAMAKTVGIPAAIGALLLIEDKIKTRGVLRPLEAEVYLPALDILQAYGIKLMEKAE 1064

The following BLAST results are available for this feature:

BLAST of HO067811 vs. SwissProt
BLAST of HO067811 vs. TrEMBL
BLAST of HO067811 vs. TAIR peptide

BLAST of HO067811 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Swissprot)
Total hits: 9


Match Name	E-value	Identity	Description
AASS_ARATH	1.217e-51	61.49	Alpha-aminoadipic semialdehyde synthase OS=Arabido...	[more]
SCPDH_DICDI	2.651e-14	33.08	Probable saccharopine dehydrogenase [NADP+, L-glut...	[more]
LYS9_YEAST	1.455e-12	33.12	Saccharopine dehydrogenase [NADP+, L-glutamate-for...	[more]
LYS9_MAGGR	3.240e-12	28.66	Saccharopine dehydrogenase [NADP+, L-glutamate-for...	[more]
AASS_BOVIN	4.232e-12	32.85	Alpha-aminoadipic semialdehyde synthase, mitochond...	[more]
AASS_RAT	5.527e-12	34.96	Alpha-aminoadipic semialdehyde synthase, mitochond...	[more]
AASS_MOUSE	2.743e-11	34.15	Alpha-aminoadipic semialdehyde synthase, mitochond...	[more]
AASS_HUMAN	6.111e-11	34.15	Alpha-aminoadipic semialdehyde synthase, mitochond...	[more]
LYS9_SCHPO	2.322e-10	27.56	Saccharopine dehydrogenase [NADP+, L-glutamate-for...	[more]

BLAST of HO067811 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
B9MT60_POPTR	2.261e-63	73.13	Predicted protein (Fragment) OS=Populus trichocarp...	[more]
B9RR16_RICCO	1.122e-62	73.91	Aminoadipic semialdehyde synthase, putative OS=Ric...	[more]
D7TC82_VITVI	3.056e-60	72.50	Whole genome shotgun sequence of line PN40024, sca...	[more]
Q8H770_GOSHI	2.050e-56	67.08	Bifunctional lysine-ketoglutarate reductase/saccha...	[more]
D7M991_ARALY	3.063e-52	62.73	Lysine-ketoglutarate reductase/saccharopine dehydr...	[more]
Q1KL68_9ORYZ	4.000e-52	62.03	Lysine ketoglutarate reductase/saccharopine dehydr...	[more]
Q8GTD2_HORVD	8.912e-52	60.76	Saccharopin dehydrogenase-like protein OS=Hordeum ...	[more]
O04709_MAIZE	1.164e-51	61.39	Lysine ketoglutarate reductase/saccharopine dehydr...	[more]
Q6K7D6_ORYSJ	5.776e-51	61.54	Putative lysine-ketoglutarate reductase/saccharopi...	[more]
B9F3L6_ORYSJ	5.776e-51	61.54	Putative uncharacterized protein OS=Oryza sativa s...	[more]

BLAST of HO067811 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide)
Total hits: 3


Match Name	E-value	Identity	Description
AT4G33150.3	1.280e-52	61.49	\| Symbols: \| lysine-ketoglutarate reductase/sacch...	[more]
AT4G33150.2	1.280e-52	61.49	\| Symbols: \| lysine-ketoglutarate reductase/sacch...	[more]
AT4G33150.1	1.280e-52	61.49	\| Symbols: \| lysine-ketoglutarate reductase/sacch...	[more]

InterPro

Analysis Name: InterProScan analysis for Cicer arietinum unigene v1
Date Performed: 2010-12-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	SEG	seg	seg	coord: 3..20 score: NAcoord: 26..39 score
None	No IPR available	GENE3D	3.30.360.10	no description	coord: 60..119 score: 3.7
None	No IPR available	PANTHER	PTHR11133	SACCHAROPINE DEHYDROGENASE	coord: 1..162 score: 2.4
None	No IPR available	PANTHER	PTHR11133:SF3	AMINOADIPIC SEMIALDEHYDE DEHYDROGENASE	coord: 1..162 score: 2.4
None	No IPR available	SUPERFAMILY	55347	Glyceraldehyde-3-phosphate dehydrogenase-like, C-terminal domain	coord: 5..105 score: 2.9
IPR005097	Saccharopine dehydrogenase / Homospermidine synthase	PFAM	PF03435	Saccharop_dh	coord: 34..155 score: 1.2

Properties


Property Name	Value
Singlet in analysis	Cicer arietinum unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>HO067811 ID=HO067811; Name=HO067811; organism=Cicer arietinum; type=EST; length=590bp
ATGGTAGTTCCGATTATTATTATTATTGTCGAAATTATTTACATTATATA
GTACATCATCCTCTCTACATCAAACAGAACACCAACTTCAAATATACAAA
TTACTCATTCTTCTCTATCAACTTGATGCCATAAGCTTGTATAATATCAA
GGGCTGGAGTGTATACTTCAGGTTGAATGGGCCTTAAAACACCTCTTGTC
TGAATTTTGTTGGTCAGTAAGAGCAGAGCTCCAACAGCAGCTGGAATACC
AACAGTAAGGGCCATGGCAGTAGTGGTTTTTCCATCAACAGTCTTCCCAA
ATTCAAGCAAAGTAGCTCTATGCTTCTCTGTAATTTGGCTGTCGGGGTAT
TCTATCTCCACTTCATGGTGCAAAAGCACCATATCCTTTTCTGTGCTGGA
GTAGGATAATCTCTCTCCCATACAGAAACAAGCAACATCAAAAGCACTTC
GGCAGGAAGCTGGGATTTCTGTTTGATCAAGAAGTCCCAAAAATATGATT
GTTTTTGCTGTCATCATTGCAGCTCCTTGATCTTTGCAATGTCCCAGTGT
CAATATCTTTTCTGTGATGTCCTCCTCTCCCATCAATGCT

Annotated Terms

The following terms have been associated with this EST:

Vocabulary: **INTERPRO**
Term	Definition
IPR005097	Sacchrp_dh_NADP

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