ES560411

EST Overview
NameES560411
Unique NameES560411
TypeEST
OrganismCicer arietinum (chickpea)
Sequence length191
Libraries
Library NameType
LIBEST_021248 Ascochyta rabiei induced librarycdna_library
Unigenes
This EST is part of the following unigenes:
Unigene NameAnalysis NameSequence type in Unigene
Cicer arietinum unigene v1Cicer arietinum unigene v1EST
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
Cicer arietinum unigene v12010-12-27
Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide2011-02-03
Homology Analysis: Cicer arietinum unigene v1 vs Trembl2010-12-28
InterProScan analysis for Cicer arietinum unigene v12010-12-29
Homology
BLAST of ES560411 vs. TrEMBL
Match: A2Q3E0_MEDTR (DNA-directed RNA polymerase, 14 to 18 kDa subunit OS=Medicago truncatula GN=MtrDRAFT_AC155881g13v1 PE=3 SV=1)

HSP 1 Score: 76.2554 bits (186), Expect = 1.152e-12
Identity = 35/43 (81.40%), Postives = 38/43 (88.37%), Query Frame = 3
Query:   60 NKNDDMTGEGEPIETEDKEDERPGERPRKTSKYMTKYERARIL 188
            NKNDD+  EGEPIETE+KED  P ERPR+TSKYMTKYERARIL
Sbjct:   33 NKNDDLDAEGEPIETEEKEDAAPVERPRRTSKYMTKYERARIL 75          
BLAST of ES560411 vs. TrEMBL
Match: B7FHE6_MEDTR (Putative uncharacterized protein OS=Medicago truncatula PE=2 SV=1)

HSP 1 Score: 74.3294 bits (181), Expect = 4.378e-12
Identity = 34/42 (80.95%), Postives = 37/42 (88.10%), Query Frame = 3
Query:   60 NKNDDMTGEGEPIETEDKEDERPGERPRKTSKYMTKYERARI 185
            NKNDD+  EGEPIETE+KED  P ERPR+TSKYMTKYERARI
Sbjct:   33 NKNDDLDAEGEPIETEEKEDVAPVERPRRTSKYMTKYERARI 74          
BLAST of ES560411 vs. TrEMBL
Match: C6SWG7_SOYBN (Putative uncharacterized protein OS=Glycine max PE=2 SV=1)

HSP 1 Score: 68.5514 bits (166), Expect = 2.402e-10
Identity = 33/43 (76.74%), Postives = 39/43 (90.70%), Query Frame = 3
Query:   60 NKNDDMTGEGEPIETEDKEDERPGERPRKTSKYMTKYERARIL 188
            NKND++TGE   I+TEDKE+E+P +RPRKTSKYMTKYERARIL
Sbjct:   32 NKNDEITGES--IDTEDKEEEQPVKRPRKTSKYMTKYERARIL 72          
BLAST of ES560411 vs. TrEMBL
Match: B9ID35_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_824910 PE=3 SV=1)

HSP 1 Score: 67.0106 bits (162), Expect = 6.989e-10
Identity = 33/43 (76.74%), Postives = 37/43 (86.05%), Query Frame = 3
Query:   60 NKNDDMTGEGEPIETEDKEDERPGERPRKTSKYMTKYERARIL 188
            N N+D TGE  PIETEDKE++ P ERPRKTSK+MTKYERARIL
Sbjct:   33 NNNEDDTGE--PIETEDKEEQAPVERPRKTSKFMTKYERARIL 73          
BLAST of ES560411 vs. TrEMBL
Match: B9I4C9_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_814294 PE=3 SV=1)

HSP 1 Score: 65.855 bits (159), Expect = 1.557e-9
Identity = 32/43 (74.42%), Postives = 36/43 (83.72%), Query Frame = 3
Query:   60 NKNDDMTGEGEPIETEDKEDERPGERPRKTSKYMTKYERARIL 188
            N  DD   +GEPIETEDKE++ P ERPRKTSK+MTKYERARIL
Sbjct:   34 NNEDD---KGEPIETEDKEEQEPVERPRKTSKFMTKYERARIL 73          
BLAST of ES560411 vs. TrEMBL
Match: B9R9F9_RICCO (DNA-directed RNA polymerase subunit rpb6, putative OS=Ricinus communis GN=RCOM_1496830 PE=3 SV=1)

HSP 1 Score: 65.0846 bits (157), Expect = 2.656e-9
Identity = 31/43 (72.09%), Postives = 36/43 (83.72%), Query Frame = 3
Query:   60 NKNDDMTGEGEPIETEDKEDERPGERPRKTSKYMTKYERARIL 188
            N N+D+ G+  PIETEDKE++   ERPRKTSKYMTKYERARIL
Sbjct:   14 NNNEDVPGD--PIETEDKEEQEAAERPRKTSKYMTKYERARIL 54          
BLAST of ES560411 vs. TrEMBL
Match: D7MRK7_ARALY (Putative uncharacterized protein OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_495261 PE=4 SV=1)

HSP 1 Score: 60.8474 bits (146), Expect = 5.009e-8
Identity = 29/42 (69.05%), Postives = 35/42 (83.33%), Query Frame = 3
Query:   63 KNDDMTGEGEPIETEDKEDERPGERPRKTSKYMTKYERARIL 188
            +NDD+ GE  P+ETEDK +  P +RPRKTSK+MTKYERARIL
Sbjct:   35 ENDDVNGE--PLETEDKVETEPVQRPRKTSKFMTKYERARIL 74          
BLAST of ES560411 vs. TrEMBL
Match: D7L835_ARALY (Putative uncharacterized protein OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_899271 PE=4 SV=1)

HSP 1 Score: 60.4622 bits (145), Expect = 6.541e-8
Identity = 29/42 (69.05%), Postives = 35/42 (83.33%), Query Frame = 3
Query:   63 KNDDMTGEGEPIETEDKEDERPGERPRKTSKYMTKYERARIL 188
            +NDD+ GE  P+ETEDK +  P +RPRKTSK+MTKYERARIL
Sbjct:   35 ENDDVNGE--PMETEDKVETEPVQRPRKTSKFMTKYERARIL 74          
BLAST of ES560411 vs. TrEMBL
Match: Q9FJ98_ARATH (DNA-directed RNA polymerase II subunit-like protein OS=Arabidopsis thaliana GN=At5g51940 PE=2 SV=1)

HSP 1 Score: 59.6918 bits (143), Expect = 1.116e-7
Identity = 29/42 (69.05%), Postives = 34/42 (80.95%), Query Frame = 3
Query:   63 KNDDMTGEGEPIETEDKEDERPGERPRKTSKYMTKYERARIL 188
            +NDD+ GE  PIE EDK +  P +RPRKTSK+MTKYERARIL
Sbjct:   35 ENDDVNGE--PIEAEDKVETEPVQRPRKTSKFMTKYERARIL 74          
BLAST of ES560411 vs. TAIR peptide
Match: AT5G51940.1 (| Symbols: NRPB6A, NRPD6A, NRPE6A | RNA polymerase Rpb6 | chr5:21104679-21105796 FORWARD LENGTH=144)

HSP 1 Score: 59.6918 bits (143), Expect = 4.256e-10
Identity = 29/42 (69.05%), Postives = 34/42 (80.95%), Query Frame = 3
Query:   63 KNDDMTGEGEPIETEDKEDERPGERPRKTSKYMTKYERARIL 188
            +NDD+ GE  PIE EDK +  P +RPRKTSK+MTKYERARIL
Sbjct:   35 ENDDVNGE--PIEAEDKVETEPVQRPRKTSKFMTKYERARIL 74          
BLAST of ES560411 vs. TAIR peptide
Match: AT2G04630.1 (| Symbols: NRPB6B, NRPE6B | RNA polymerase Rpb6 | chr2:1619168-1620246 REVERSE LENGTH=144)

HSP 1 Score: 56.225 bits (134), Expect = 4.705e-9
Identity = 27/42 (64.29%), Postives = 34/42 (80.95%), Query Frame = 3
Query:   63 KNDDMTGEGEPIETEDKEDERPGERPRKTSKYMTKYERARIL 188
            +NDD+  +  P+ETEDK +  P +RPRKTSK+MTKYERARIL
Sbjct:   35 ENDDVNVD--PLETEDKVETEPVQRPRKTSKFMTKYERARIL 74          
The following BLAST results are available for this feature:
BLAST of ES560411 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Trembl)
Total hits: 9
Match NameE-valueIdentityDescription
A2Q3E0_MEDTR1.152e-1281.40DNA-directed RNA polymerase, 14 to 18 kDa subunit ... [more]
B7FHE6_MEDTR4.378e-1280.95Putative uncharacterized protein OS=Medicago trunc... [more]
C6SWG7_SOYBN2.402e-1076.74Putative uncharacterized protein OS=Glycine max PE... [more]
B9ID35_POPTR6.989e-1076.74Predicted protein OS=Populus trichocarpa GN=POPTRD... [more]
B9I4C9_POPTR1.557e-974.42Predicted protein OS=Populus trichocarpa GN=POPTRD... [more]
B9R9F9_RICCO2.656e-972.09DNA-directed RNA polymerase subunit rpb6, putative... [more]
D7MRK7_ARALY5.009e-869.05Putative uncharacterized protein OS=Arabidopsis ly... [more]
D7L835_ARALY6.541e-869.05Putative uncharacterized protein OS=Arabidopsis ly... [more]
Q9FJ98_ARATH1.116e-769.05DNA-directed RNA polymerase II subunit-like protei... [more]
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BLAST of ES560411 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide)
Total hits: 2
Match NameE-valueIdentityDescription
AT5G51940.14.256e-1069.05| Symbols: NRPB6A, NRPD6A, NRPE6A | RNA polymerase... [more]
AT2G04630.14.705e-964.29| Symbols: NRPB6B, NRPE6B | RNA polymerase Rpb6 | ... [more]
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InterPro
Analysis Name: InterProScan analysis for Cicer arietinum unigene v1
Date Performed: 2010-12-29
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR012293RNA polymerase subunit, RPB6/omegaGENE3D3.90.940.10no descriptioncoord: 8..62
score: 2.6
NoneNo IPR availableSEGsegsegcoord: 1..19
score
NoneNo IPR availableSEGsegsegcoord: 15..63
score
NoneNo IPR availableSEGsegsegcoord: 20..31
score: NAcoord: 48..63
score

Properties
Property NameValue
Singlet in analysisCicer arietinum unigene v1
Sequences
The following sequences are available for this feature:

EST sequence

>ES560411 ID=ES560411; Name=ES560411; organism=Cicer arietinum; type=EST; length=191bp
ACGAGGAGGAGCCACCAGAGCCCGAGATTGAGGAAGGTGCAGAGGAGGAG
GATCTCGAGAACAAAAATGATGACATGACTGGAGAAGGAGAACCAATTGA
AACTGAGGATAAGGAAGATGAGCGACCAGGAGAGCGTCCTCGAAAGACAT
CGAAATATATGACCAAGTATGAGCGTGCTAGGATTCTCGAT
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Annotated Terms
The following terms have been associated with this EST:
Vocabulary: Molecular Function
TermDefinition
GO:0003677DNA binding
GO:0003899DNA-directed 5'-3' RNA polymerase activity
Vocabulary: Cellular Component
TermDefinition
GO:0030880RNA polymerase complex
Vocabulary: INTERPRO
TermDefinition
IPR012293RNAP_RPB6_omega