HO064263

EST Overview


Name	HO064263
Unique Name	HO064263
Type	EST
Organism	Cicer arietinum (chickpea)
Sequence length	689

Libraries


Library Name	Type
LIBEST_026411 Chickpea drought stressed cDNA SSH library AR2	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Cicer arietinum unigene v1	Cicer arietinum unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Cicer arietinum unigene v1	2010-12-27
Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide	2011-02-03
KEGG analysis for Cicer arietinum unigene v1	2011-01-05
Homology Analysis: Cicer arietinum unigene v1 vs Swissprot	2010-12-28
Homology Analysis: Cicer arietinum unigene v1 vs Trembl	2010-12-28
InterProScan analysis for Cicer arietinum unigene v1	2010-12-29

Homology

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BLAST of HO064263 vs. SwissProt
Match: AKH1_ARATH (Bifunctional aspartokinase/homoserine dehydrogenase 1, chloroplastic OS=Arabidopsis thaliana GN=AKHSDH1 PE=1 SV=1)

HSP 1 Score: 306.22 bits (783), Expect = 1.058e-82
Identity = 155/190 (81.58%), Postives = 172/190 (90.53%), Query Frame = -2

Query:    8 FGAVKRCWS*CIMISQASSEHSVCFAVPEKEVKAVAEALQSRFRQALDNGRLSQVAVIPNCSILAAVGQKMASTPGVSATLFNALAKANINVRAIAQGCSEYNITVVVKREDCIKALRAVHSRFYLSRTTIAMGVIGPGLIGSTPLDQLRDQASVLKEEFNIDLRVMGILGSKSMLLSDVGIDLARWKEL 577
            F AVK   +  IMISQASSEHSVCFAVPEKEVKAV+EAL SRFRQAL  GRLSQ+ +IPNCSILAAVGQKMASTPGVSAT FNALAKANIN+RAIAQGCSE+NITVVVKREDCI+ALRAVHSRFYLSRTT+A+G+IGPGLIG T LDQ+RDQA+VLKEEF IDLRV+GI GS  ML+S+ GIDL+RW+EL
Sbjct:  423 FSAVKEVGANVIMISQASSEHSVCFAVPEKEVKAVSEALNSRFRQALAGGRLSQIEIIPNCSILAAVGQKMASTPGVSATFFNALAKANINIRAIAQGCSEFNITVVVKREDCIRALRAVHSRFYLSRTTLAVGIIGPGLIGGTLLDQIRDQAAVLKEEFKIDLRVIGITGSSKMLMSESGIDLSRWREL 612

[Close]

BLAST of HO064263 vs. SwissProt
Match: AKH2_ARATH (Bifunctional aspartokinase/homoserine dehydrogenase 2, chloroplastic OS=Arabidopsis thaliana GN=AKHSDH2 PE=1 SV=1)

HSP 1 Score: 305.064 bits (780), Expect = 2.357e-82
Identity = 153/190 (80.53%), Postives = 174/190 (91.58%), Query Frame = -2

Query:    8 FGAVKRCWS*CIMISQASSEHSVCFAVPEKEVKAVAEALQSRFRQALDNGRLSQVAVIPNCSILAAVGQKMASTPGVSATLFNALAKANINVRAIAQGCSEYNITVVVKREDCIKALRAVHSRFYLSRTTIAMGVIGPGLIGSTPLDQLRDQASVLKEEFNIDLRVMGILGSKSMLLSDVGIDLARWKEL 577
            FG VK   +  IMISQASSEHSVCFAVPEKEV AV+EAL+SRF +AL  GRLSQ+ VIPNCSILAAVGQKMASTPGVS TLF+ALAKANINVRAI+QGCSEYN+TVV+KRED +KALRAVHSRF+LSRTT+AMG++GPGLIG+T LDQLRDQA+VLK+EFNIDLRV+GI GSK MLLSD+GIDL+RW+EL
Sbjct:  428 FGCVKDVGANVIMISQASSEHSVCFAVPEKEVNAVSEALRSRFSEALQAGRLSQIEVIPNCSILAAVGQKMASTPGVSCTLFSALAKANINVRAISQGCSEYNVTVVIKREDSVKALRAVHSRFFLSRTTLAMGIVGPGLIGATLLDQLRDQAAVLKQEFNIDLRVLGITGSKKMLLSDIGIDLSRWREL 617

[Close]

BLAST of HO064263 vs. SwissProt
Match: AKH_DAUCA (Bifunctional aspartokinase/homoserine dehydrogenase, chloroplastic (Fragment) OS=Daucus carota PE=1 SV=1)

HSP 1 Score: 300.442 bits (768), Expect = 5.805e-81
Identity = 151/192 (78.65%), Postives = 175/192 (91.15%), Query Frame = -2

Query:    2 FGAVKRCWS*CIMISQASSEHSVCFAVPEKEVKAVAEALQSRFRQALDNGRLSQVAVIPNCSILAAVGQKMASTPGVSATLFNALAKANINVRAIAQGCSEYNITVVVKREDCIKALRAVHSRFYLSRTTIAMGVIGPGLIGSTPLDQLRDQASVLKEEFNIDLRVMGILGSKSMLLSDVGIDLARWKELRE 577
            FGAVK   +  IMISQASSEHS+CFAVPE EVKAVA+AL++RFRQALD GRLSQVA  PNCSILA VGQKMASTPGVSATLFNALAKANINVRAIAQGC+EYNITVV+ REDC++AL+AVHSRFYLSRTTIA+G++GPGLIG+T LDQLRDQA++LKE   IDLRVMGI GS++MLLS+ GIDL+RW+E+++
Sbjct:  433 FGAVKDVGANVIMISQASSEHSICFAVPESEVKAVAKALEARFRQALDAGRLSQVANNPNCSILATVGQKMASTPGVSATLFNALAKANINVRAIAQGCTEYNITVVLSREDCVRALKAVHSRFYLSRTTIAVGIVGPGLIGATLLDQLRDQAAILKENSKIDLRVMGITGSRTMLLSETGIDLSRWREVQK 624

[Close]

BLAST of HO064263 vs. SwissProt
Match: AKH1_MAIZE (Bifunctional aspartokinase/homoserine dehydrogenase 1, chloroplastic OS=Zea mays GN=AKHSDH1 PE=2 SV=1)

HSP 1 Score: 281.952 bits (720), Expect = 2.136e-75
Identity = 142/189 (75.13%), Postives = 167/189 (88.36%), Query Frame = -2

Query:   11 FGAVKRCWS*CIMISQASSEHSVCFAVPEKEVKAVAEALQSRFRQALDNGRLSQVAVIPNCSILAAVGQKMASTPGVSATLFNALAKANINVRAIAQGCSEYNITVVVKREDCIKALRAVHSRFYLSRTTIAMGVIGPGLIGSTPLDQLRDQASVLKEEFNIDLRVMGILGSKSMLLSDVGIDLARWKE 577
            FGAVK   +  IMISQASSEHSVCFAVPEKEV  V+ AL +RFR+AL  GRLS+V VI NCSILA VG +MASTPGVSATLF+ALAKANINVRAIAQGCSEYNIT+V+K+EDC++ALRA HSRF+LS+TT+A+G+IGPGLIG T L+QL+DQA+VLKE  NIDLRVMGI GS++MLLSD+G+DL +WKE
Sbjct:  432 FGAVKDVGANVIMISQASSEHSVCFAVPEKEVALVSAALHARFREALAAGRLSKVEVIHNCSILATVGLRMASTPGVSATLFDALAKANINVRAIAQGCSEYNITIVLKQEDCVRALRAAHSRFFLSKTTLAVGIIGPGLIGRTLLNQLKDQAAVLKENMNIDLRVMGIAGSRTMLLSDIGVDLTQWKE 620

[Close]

BLAST of HO064263 vs. SwissProt
Match: AKH2_MAIZE (Bifunctional aspartokinase/homoserine dehydrogenase 2, chloroplastic OS=Zea mays GN=AKHSDH2 PE=2 SV=1)

HSP 1 Score: 266.159 bits (679), Expect = 1.213e-70
Identity = 136/190 (71.58%), Postives = 160/190 (84.21%), Query Frame = -2

Query:    8 FGAVKRCWS*CIMISQASSEHSVCFAVPEKEVKAVAEALQSRFRQALDNGRLSQVAVIPNCSILAAVGQKMASTPGVSATLFNALAKANINVRAIAQGCSEYNITVVVKREDCIKALRAVHSRFYLSRTTIAMGVIGPGLIGSTPLDQLRDQASVLKEEFNIDLRVMGILGSKSMLLSDVGIDLARWKEL 577
            F AVK   +  IMISQASSEHSVCFAVPEKEV  V+  L  RFR+AL  GRLS+V VI  CSILAAVG +MASTPGVSA LF+ALAKANINVRAIAQGCSEYNITVV+K++DC++ALRA HSRF+LS+TT+A+G+IGPGLIG   L+QL++Q +VLKE  NIDLRV+GI GS +MLLSD GIDL +WK+L
Sbjct:  429 FSAVKDVGANVIMISQASSEHSVCFAVPEKEVAVVSAELHDRFREALAAGRLSKVEVINGCSILAAVGLRMASTPGVSAILFDALAKANINVRAIAQGCSEYNITVVLKQQDCVRALRAAHSRFFLSKTTLAVGIIGPGLIGGALLNQLKNQTAVLKENMNIDLRVIGITGSSTMLLSDTGIDLTQWKQL 618

[Close]

BLAST of HO064263 vs. SwissProt
Match: AK1H_ECOLI (Bifunctional aspartokinase/homoserine dehydrogenase 1 OS=Escherichia coli (strain K12) GN=thrA PE=1 SV=2)

HSP 1 Score: 132.494 bits (332), Expect = 2.093e-30
Identity = 75/189 (39.68%), Postives = 112/189 (59.26%), Query Frame = -2

Query:   11 FGAVKRCWS*CIMISQASSEHSVCFAVPEKEVKAVAEALQSRFRQALDNGRLSQVAVIPNCSILAAVGQKMASTPGVSATLFNALAKANINVRAIAQGCSEYNITVVVKREDCIKALRAVHSRFYLSRTTIAMGVIGPGLIGSTPLDQLRDQASVLKEEFNIDLRVMGILGSKSMLLSDVGIDLARWKE 577
            F A+ R     ++I+Q+SSE+S+ F VP+ +      A+Q  F   L  G L  +AV    +I++ VG  M +  G+SA  F ALA+ANIN+ AIAQG SE +I+VVV  +D    +R  H   + +   I + VIG G +G   L+QL+ Q S LK + +IDLRV G+  SK++L +  G++L  W+E
Sbjct:  336 FAAMSRARISVVLITQSSSEYSISFCVPQSDCVRAERAMQEEFYLELKEGLLEPLAVTERLAIISVVGDGMRTLRGISAKFFAALARANINIVAIAQGSSERSISVVVNNDDATTGVRVTHQMLFNTDQVIEVFVIGVGGVGGALLEQLKRQQSWLKNK-HIDLRVCGVANSKALLTNVHGLNLENWQE 523

[Close]

BLAST of HO064263 vs. SwissProt
Match: AKH_HAEIN (Bifunctional aspartokinase/homoserine dehydrogenase OS=Haemophilus influenzae GN=thrA PE=3 SV=1)

HSP 1 Score: 124.02 bits (310), Expect = 7.446e-28
Identity = 64/177 (36.16%), Postives = 109/177 (61.58%), Query Frame = -2

Query:   14 IMISQASSEHSVCFAVPEKEVKAVAEALQSRFRQALDNGRLSQVAVIPNCSILAAVGQKMASTPGVSATLFNALAKANINVRAIAQGCSEYNITVVVKREDCIKALRAVHSRFYLSRTTIAMGVIGPGLIGSTPLDQLRDQASVLKEEFNIDLRVMGILGSKSMLLSDVGIDLARWK 544
            I+I+Q+SSE+S+ F VP K  +     L++ F   L+  +L  + VI + SI++ VG  M    G++A  F+ALA+ANI++ AIAQG SE +I+ VV +   I+A++A H   + ++  + M ++G G +G   ++Q++ Q   L ++ N+++RV  I  S  MLL + G++L  WK
Sbjct:  347 ILITQSSSEYSISFCVPVKSAEVAKTVLETEFANELNEHQLEPIEVIKDLSIISVVGDGMKQAKGIAARFFSALAQANISIVAIAQGSSERSISAVVPQNKAIEAVKATHQALFNNKKVVDMFLVGVGGVGGELIEQVKRQKEYLAKK-NVEIRVCAIANSNRMLLDENGLNLEDWK 522

[Close]

BLAST of HO064263 vs. SwissProt
Match: AK1H_SERMA (Bifunctional aspartokinase/homoserine dehydrogenase 1 OS=Serratia marcescens GN=thrA PE=3 SV=1)

HSP 1 Score: 122.865 bits (307), Expect = 1.659e-27
Identity = 71/189 (37.57%), Postives = 111/189 (58.73%), Query Frame = -2

Query:   11 FGAVKRCWS*CIMISQASSEHSVCFAVPEKEVKAVAEALQSRFRQALDNGRLSQVAVIPNCSILAAVGQKMASTPGVSATLFNALAKANINVRAIAQGCSEYNITVVVKREDCIKALRAVHSRFYLSRTTIAMGVIGPGLIGSTPLDQLRDQASVLKEEFNIDLRVMGILGSKSMLLSDVGIDLARWKE 577
            F  + R     ++I+Q+SSE+S+ F VP+ E++    AL+  F   L +G L  + V+   +I++ VG  M +  G+SA  F+ALA+ANIN+ AIAQG SE +I+VVV  +     +R  H   + +   I + VIG G +G   ++Q+  Q   LK++ +IDLRV GI  S+ ML +  GI L  W++
Sbjct:  336 FAVMSRAGISVVLITQSSSEYSISFCVPQGELQRARRALEEEFYLELKDGVLDPLDVMERLAIISVVGDGMRTLRGISARFFSALARANINIVAIAQGSSERSISVVVSNDSATTGVRVSHQMLFNTDQVIEVFVIGVGGVGGALIEQIYRQQPWLKQK-HIDLRVCGIANSRVMLTNVHGIALDSWRD 523

[Close]

BLAST of HO064263 vs. SwissProt
Match: AKH_BUCAI (Bifunctional aspartokinase/homoserine dehydrogenase OS=Buchnera aphidicola subsp. Acyrthosiphon pisum GN=thrA PE=3 SV=1)

HSP 1 Score: 114.39 bits (285), Expect = 5.900e-25
Identity = 62/177 (35.03%), Postives = 109/177 (61.58%), Query Frame = -2

Query:   14 IMISQASSEHSVCFAVPEKEVKAVAEALQSRFRQALDNGRLSQVAVIPNCSILAAVGQKMASTPGVSATLFNALAKANINVRAIAQGCSEYNITVVVKREDCIKALRAVHSRFYLSRTTIAMGVIGPGLIGSTPLDQLRDQASVLKEEFNIDLRVMGILGSKSMLLSDVGIDLARWK 544
            ++I+Q+SSE+ + F V E ++  +       F+  L +G L+   +  N SIL+ VG  +     +++ +F+ L    INV AI+QG S+++I++V+K+E+ +KA++ VH+  + ++ TI M +IG G IGST L+Q+  Q   L ++ NI++++  I  SK +LL D   DL+ WK
Sbjct:  348 LLITQSSSENKINFCVYEHDIYKILYLFNKEFQLELKDGLLNPFKIKKNLSILSIVGSNIYKKHNIASKIFSVLGALKINVIAISQGSSKHSISLVIKKENILKAVQHVHNTLFFNKKTIHMFLIGIGGIGSTLLNQILKQKQFLDKK-NIEIKICAIANSKKILLLDNTNDLSNWK 523

[Close]

BLAST of HO064263 vs. SwissProt
Match: AKH_BUCAP (Bifunctional aspartokinase/homoserine dehydrogenase OS=Buchnera aphidicola subsp. Schizaphis graminum GN=thrA PE=3 SV=1)

HSP 1 Score: 101.679 bits (252), Expect = 3.957e-21
Identity = 52/178 (29.21%), Postives = 103/178 (57.87%), Query Frame = -2

Query:   11 IMISQASSEHSVCFAVPEKEVKAVAEALQSRFRQALDNGRLSQVAVIPNCSILAAVGQKMASTPGVSATLFNALAKANINVRAIAQGCSEYNITVVVKREDCIKALRAVHSRFYLSRTTIAMGVIGPGLIGSTPLDQLRDQASVLKEEFNIDLRVMGILGSKSMLLSDVGIDLARWKE 544
            I+I Q+SSE+ + F   EK++  +   L+  F   +  G L+   ++ N +IL+ +G  ++    +++ +F++L  + INV AIA G S+++I++V+K+E+ ++ ++ +H+  +  +T I + +IG G +G   L Q+  Q   L ++ NI ++   I  SK +L     I+L  W+E
Sbjct:  346 ILIIQSSSENQINFCTFEKDIDYILLILKKEFTLEIKEGLLNDFNIVKNLTILSVIGSNISEKNNIASKIFSSLGSSKINVLAIAHGSSKHSISIVIKKENLLQGIQNIHNTLFFKKTIINVFLIGIGGVGKALLKQILKQEKFLDQK-NIKIQFRMIANSKKLLFLKNSINLNNWEE 522

[Close]

BLAST of HO064263 vs. TrEMBL
Match: O65027_SOYBN (Aspartokinase-homoserine dehydrogenase (Fragment) OS=Glycine max GN=AK-HSDH PE=4 SV=1)

HSP 1 Score: 345.125 bits (884), Expect = 3.049e-93
Identity = 181/192 (94.27%), Postives = 185/192 (96.35%), Query Frame = -2

Query:    2 FGAVKRCWS*CIMISQASSEHSVCFAVPEKEVKAVAEALQSRFRQALDNGRLSQVAVIPNCSILAAVGQKMASTPGVSATLFNALAKANINVRAIAQGCSEYNITVVVKREDCIKALRAVHSRFYLSRTTIAMGVIGPGLIGSTPLDQLRDQASVLKEEFNIDLRVMGILGSKSMLLSDVGIDLARWKELRE 577
            FGAVK   +  IMISQASSEHSVCFAVPEKEVKAVAEALQSRFRQALDNGRLSQVAVIPNCSILAAVGQKMASTPGVSA+LFNALAKANINVRAIAQGCSEYNITVVVKREDCIKALRAVHSRFYLSRTTIAMG+IGPGLIGST LDQLRDQAS LKEEFNIDLRVMGILGSKSMLLSDVGIDLARW+ELRE
Sbjct:  428 FGAVKDVGANVIMISQASSEHSVCFAVPEKEVKAVAEALQSRFRQALDNGRLSQVAVIPNCSILAAVGQKMASTPGVSASLFNALAKANINVRAIAQGCSEYNITVVVKREDCIKALRAVHSRFYLSRTTIAMGIIGPGLIGSTLLDQLRDQASTLKEEFNIDLRVMGILGSKSMLLSDVGIDLARWRELRE 619

[Close]

BLAST of HO064263 vs. TrEMBL
Match: O63067_SOYBN (Aspartokinase-homoserine dehydrogenase OS=Glycine max GN=AK-HSDH PE=2 SV=1)

HSP 1 Score: 343.584 bits (880), Expect = 8.872e-93
Identity = 180/192 (93.75%), Postives = 185/192 (96.35%), Query Frame = -2

Query:    2 FGAVKRCWS*CIMISQASSEHSVCFAVPEKEVKAVAEALQSRFRQALDNGRLSQVAVIPNCSILAAVGQKMASTPGVSATLFNALAKANINVRAIAQGCSEYNITVVVKREDCIKALRAVHSRFYLSRTTIAMGVIGPGLIGSTPLDQLRDQASVLKEEFNIDLRVMGILGSKSMLLSDVGIDLARWKELRE 577
            FGAVK   +  IMISQASSEHSVCFAVPEKEVKAVAEALQSRFRQALDNGRLSQVAVIPNCSILAAVGQKMASTPGVSA+LFNALAKANINVRAIAQGCSEYNITVVVKREDCIKALRAVHSRFYLSRTTIAMG+IGPGLIGST L+QLRDQAS LKEEFNIDLRVMGILGSKSMLLSDVGIDLARW+ELRE
Sbjct:  428 FGAVKDVGANVIMISQASSEHSVCFAVPEKEVKAVAEALQSRFRQALDNGRLSQVAVIPNCSILAAVGQKMASTPGVSASLFNALAKANINVRAIAQGCSEYNITVVVKREDCIKALRAVHSRFYLSRTTIAMGIIGPGLIGSTLLEQLRDQASTLKEEFNIDLRVMGILGSKSMLLSDVGIDLARWRELRE 619

[Close]

BLAST of HO064263 vs. TrEMBL
Match: B9SHP2_RICCO (Aspartate kinase, putative OS=Ricinus communis GN=RCOM_0742100 PE=4 SV=1)

HSP 1 Score: 322.783 bits (826), Expect = 1.621e-86
Identity = 168/190 (88.42%), Postives = 178/190 (93.68%), Query Frame = -2

Query:    8 FGAVKRCWS*CIMISQASSEHSVCFAVPEKEVKAVAEALQSRFRQALDNGRLSQVAVIPNCSILAAVGQKMASTPGVSATLFNALAKANINVRAIAQGCSEYNITVVVKREDCIKALRAVHSRFYLSRTTIAMGVIGPGLIGSTPLDQLRDQASVLKEEFNIDLRVMGILGSKSMLLSDVGIDLARWKEL 577
            FGAVK   +  IMISQASSEHSVCFAVPEKEV AVAEALQSRFRQAL  GRLSQVA+IPNCSILAAVGQKMASTPGVSATLFNALAKA+INVRAIAQGCSEYNITVVVKREDCIKALRAVHSRFYLS+TTIAMG+IGPGLIG T LDQLRDQA+VLKEEFNIDLRVMGI GS+ MLLS+VGIDL+RW+EL
Sbjct:  432 FGAVKDVGANVIMISQASSEHSVCFAVPEKEVNAVAEALQSRFRQALHAGRLSQVAIIPNCSILAAVGQKMASTPGVSATLFNALAKASINVRAIAQGCSEYNITVVVKREDCIKALRAVHSRFYLSKTTIAMGIIGPGLIGGTLLDQLRDQAAVLKEEFNIDLRVMGITGSRRMLLSEVGIDLSRWREL 621

[Close]

BLAST of HO064263 vs. TrEMBL
Match: D7TL78_VITVI (Whole genome shotgun sequence of line PN40024, scaffold_82.assembly12x (Fragment) OS=Vitis vinifera GN=VIT_00036290001 PE=4 SV=1)

HSP 1 Score: 316.62 bits (810), Expect = 1.161e-84
Identity = 162/190 (85.26%), Postives = 178/190 (93.68%), Query Frame = -2

Query:    8 FGAVKRCWS*CIMISQASSEHSVCFAVPEKEVKAVAEALQSRFRQALDNGRLSQVAVIPNCSILAAVGQKMASTPGVSATLFNALAKANINVRAIAQGCSEYNITVVVKREDCIKALRAVHSRFYLSRTTIAMGVIGPGLIGSTPLDQLRDQASVLKEEFNIDLRVMGILGSKSMLLSDVGIDLARWKEL 577
            F AVK   +  IMISQASSEHSVCFAVPEKEV+AVAEALQSRFRQALD GRLSQVAV+PNCSILA VGQ+MASTPGVSA+LF+ALAKANIN+RAIAQGCSEYNITVVVKREDCI+AL+AVHSRFYLSRTTIAMG+IGPGLIG T LDQLRDQA+VLKE+FNIDLRVMGI GS++MLLSD GIDL+RW+EL
Sbjct:  383 FSAVKDVGANVIMISQASSEHSVCFAVPEKEVEAVAEALQSRFRQALDAGRLSQVAVVPNCSILATVGQRMASTPGVSASLFSALAKANINIRAIAQGCSEYNITVVVKREDCIRALKAVHSRFYLSRTTIAMGIIGPGLIGGTLLDQLRDQAAVLKEDFNIDLRVMGITGSRTMLLSDSGIDLSRWREL 572

[Close]

BLAST of HO064263 vs. TrEMBL
Match: A5APD8_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_034964 PE=4 SV=1)

HSP 1 Score: 316.62 bits (810), Expect = 1.161e-84
Identity = 162/190 (85.26%), Postives = 178/190 (93.68%), Query Frame = -2

Query:    8 FGAVKRCWS*CIMISQASSEHSVCFAVPEKEVKAVAEALQSRFRQALDNGRLSQVAVIPNCSILAAVGQKMASTPGVSATLFNALAKANINVRAIAQGCSEYNITVVVKREDCIKALRAVHSRFYLSRTTIAMGVIGPGLIGSTPLDQLRDQASVLKEEFNIDLRVMGILGSKSMLLSDVGIDLARWKEL 577
            F AVK   +  IMISQASSEHSVCFAVPEKEV+AVAEALQSRFRQALD GRLSQVAV+PNCSILA VGQ+MASTPGVSA+LF+ALAKANIN+RAIAQGCSEYNITVVVKREDCI+AL+AVHSRFYLSRTTIAMG+IGPGLIG T LDQLRDQA+VLKE+FNIDLRVMGI GS++MLLSD GIDL+RW+EL
Sbjct:  353 FSAVKDVGANVIMISQASSEHSVCFAVPEKEVEAVAEALQSRFRQALDAGRLSQVAVVPNCSILATVGQRMASTPGVSASLFSALAKANINIRAIAQGCSEYNITVVVKREDCIRALKAVHSRFYLSRTTIAMGIIGPGLIGGTLLDQLRDQAAVLKEDFNIDLRVMGITGSRTMLLSDSGIDLSRWREL 542

[Close]

BLAST of HO064263 vs. TrEMBL
Match: B9MWJ3_POPTR (Predicted protein (Fragment) OS=Populus trichocarpa GN=POPTRDRAFT_288174 PE=4 SV=1)

HSP 1 Score: 311.997 bits (798), Expect = 2.861e-83
Identity = 161/190 (84.74%), Postives = 178/190 (93.68%), Query Frame = -2

Query:    8 FGAVKRCWS*CIMISQASSEHSVCFAVPEKEVKAVAEALQSRFRQALDNGRLSQVAVIPNCSILAAVGQKMASTPGVSATLFNALAKANINVRAIAQGCSEYNITVVVKREDCIKALRAVHSRFYLSRTTIAMGVIGPGLIGSTPLDQLRDQASVLKEEFNIDLRVMGILGSKSMLLSDVGIDLARWKEL 577
            FGAVK   +  I+ISQASSEHSVCFAVPEKEV AVAEAL+SRF +AL+ GRLSQVAVIPNCSILAAVGQKMAST GVSATLFNALAKANINVRAIAQGCSEYNITVV+KR DCI+ALRAVHSRFYLS+TTIAMG+IGPGLIG+T LDQLRDQA+VLKE+FNIDLRVMGI GS++MLL+DVGIDL+RW+EL
Sbjct:  355 FGAVKDVGANVIVISQASSEHSVCFAVPEKEVAAVAEALKSRFHEALNAGRLSQVAVIPNCSILAAVGQKMASTHGVSATLFNALAKANINVRAIAQGCSEYNITVVIKRGDCIRALRAVHSRFYLSKTTIAMGIIGPGLIGATLLDQLRDQAAVLKEDFNIDLRVMGITGSRTMLLNDVGIDLSRWREL 544

[Close]

BLAST of HO064263 vs. TrEMBL
Match: B9IQ92_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_780515 PE=4 SV=1)

HSP 1 Score: 311.227 bits (796), Expect = 4.880e-83
Identity = 161/190 (84.74%), Postives = 177/190 (93.16%), Query Frame = -2

Query:    8 FGAVKRCWS*CIMISQASSEHSVCFAVPEKEVKAVAEALQSRFRQALDNGRLSQVAVIPNCSILAAVGQKMASTPGVSATLFNALAKANINVRAIAQGCSEYNITVVVKREDCIKALRAVHSRFYLSRTTIAMGVIGPGLIGSTPLDQLRDQASVLKEEFNIDLRVMGILGSKSMLLSDVGIDLARWKEL 577
            FGAVK   +  IMISQASSEHSVCFAVPEKEV AVAEAL+SRF +AL+ GRLSQVAVI NCSILAAVGQKMASTPGVSATLFNALAKANINVRAIAQGCSEYNITVV+KREDCI+ALRAVHSRFYLS+TTIAMG+IGPGLIG+T LDQLRDQA+ LKE+FNIDLRVMGI GS++MLLSDV IDL++W+EL
Sbjct:  341 FGAVKDVGANVIMISQASSEHSVCFAVPEKEVTAVAEALKSRFHEALNAGRLSQVAVIRNCSILAAVGQKMASTPGVSATLFNALAKANINVRAIAQGCSEYNITVVIKREDCIRALRAVHSRFYLSKTTIAMGIIGPGLIGATLLDQLRDQAAFLKEDFNIDLRVMGITGSRTMLLSDVEIDLSKWREL 530

[Close]

BLAST of HO064263 vs. TrEMBL
Match: D7MG88_ARALY (AK-HSDH/AK-HSDH II OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_492891 PE=4 SV=1)

HSP 1 Score: 305.449 bits (781), Expect = 2.678e-81
Identity = 154/190 (81.05%), Postives = 175/190 (92.11%), Query Frame = -2

Query:    8 FGAVKRCWS*CIMISQASSEHSVCFAVPEKEVKAVAEALQSRFRQALDNGRLSQVAVIPNCSILAAVGQKMASTPGVSATLFNALAKANINVRAIAQGCSEYNITVVVKREDCIKALRAVHSRFYLSRTTIAMGVIGPGLIGSTPLDQLRDQASVLKEEFNIDLRVMGILGSKSMLLSDVGIDLARWKEL 577
            FG VK   +  IMISQASSEHSVCFAVPEKEV AV+EAL+SRF +AL  GRLSQ+ VIPNCSILAAVGQKMASTPGVS TLF+ALAKANINVRAI+QGCSEYN+TVV+KRED +KALRAVHSRF+LSRTT+AMG+IGPGLIG+T LDQLRDQA+VLK++FNIDLRVMGI GSK+MLLSD+GIDL+RW+EL
Sbjct:  433 FGCVKDVGANVIMISQASSEHSVCFAVPEKEVNAVSEALRSRFSEALQAGRLSQIEVIPNCSILAAVGQKMASTPGVSCTLFSALAKANINVRAISQGCSEYNVTVVIKREDSVKALRAVHSRFFLSRTTLAMGIIGPGLIGATLLDQLRDQAAVLKQKFNIDLRVMGITGSKTMLLSDIGIDLSRWREL 622

[Close]

BLAST of HO064263 vs. TrEMBL
Match: Q0WRP9_ARATH (Aspartate kinase-homoserine dehydrogenase-like protein OS=Arabidopsis thaliana GN=At4g19710 PE=2 SV=1)

HSP 1 Score: 305.064 bits (780), Expect = 3.497e-81
Identity = 153/190 (80.53%), Postives = 174/190 (91.58%), Query Frame = -2

Query:    8 FGAVKRCWS*CIMISQASSEHSVCFAVPEKEVKAVAEALQSRFRQALDNGRLSQVAVIPNCSILAAVGQKMASTPGVSATLFNALAKANINVRAIAQGCSEYNITVVVKREDCIKALRAVHSRFYLSRTTIAMGVIGPGLIGSTPLDQLRDQASVLKEEFNIDLRVMGILGSKSMLLSDVGIDLARWKEL 577
            FG VK   +  IMISQASSEHSVCFAVPEKEV AV+EAL+SRF +AL  GRLSQ+ VIPNCSILAAVGQKMASTPGVS TLF+ALAKANINVRAI+QGCSEYN+TVV+KRED +KALRAVHSRF+LSRTT+AMG++GPGLIG+T LDQLRDQA+VLK+EFNIDLRV+GI GSK MLLSD+GIDL+RW+EL
Sbjct:  428 FGCVKDVGANVIMISQASSEHSVCFAVPEKEVNAVSEALRSRFSEALQAGRLSQIEVIPNCSILAAVGQKMASTPGVSCTLFSALAKANINVRAISQGCSEYNVTVVIKREDSVKALRAVHSRFFLSRTTLAMGIVGPGLIGATLLDQLRDQAAVLKQEFNIDLRVLGITGSKKMLLSDIGIDLSRWREL 617

[Close]

BLAST of HO064263 vs. TrEMBL
Match: D7KK95_ARALY (AK-HSDH I OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_473611 PE=4 SV=1)

HSP 1 Score: 301.597 bits (771), Expect = 3.866e-80
Identity = 152/190 (80.00%), Postives = 172/190 (90.53%), Query Frame = -2

Query:    8 FGAVKRCWS*CIMISQASSEHSVCFAVPEKEVKAVAEALQSRFRQALDNGRLSQVAVIPNCSILAAVGQKMASTPGVSATLFNALAKANINVRAIAQGCSEYNITVVVKREDCIKALRAVHSRFYLSRTTIAMGVIGPGLIGSTPLDQLRDQASVLKEEFNIDLRVMGILGSKSMLLSDVGIDLARWKEL 577
            F AVK   +  IMISQASSEHSVCFAVPEKEVKAV+EAL SRFRQAL  GRLSQ+ +IPNCSILAAVGQKMASTPGVSAT F+ALAKANIN+RAIAQGCSE+NITVVVKREDCI+ALRAVHSRFYLSRTT+A+G+IGPGLIG T LDQ+RDQA+VLKEE+ IDLRV+GI GS  ML+S+ GIDL+RW++L
Sbjct:  423 FSAVKEVGANVIMISQASSEHSVCFAVPEKEVKAVSEALNSRFRQALAGGRLSQIEIIPNCSILAAVGQKMASTPGVSATFFSALAKANINIRAIAQGCSEFNITVVVKREDCIRALRAVHSRFYLSRTTLAVGIIGPGLIGGTLLDQIRDQAAVLKEEYKIDLRVIGITGSSKMLMSESGIDLSRWRKL 612

[Close]

BLAST of HO064263 vs. TAIR peptide
Match: AT1G31230.1 (| Symbols: AK-HSDH I, AK-HSDH | aspartate kinase-homoserine dehydrogenase i | chr1:11158744-11163055 REVERSE LENGTH=911)

HSP 1 Score: 306.22 bits (783), Expect = 1.068e-83
Identity = 155/190 (81.58%), Postives = 172/190 (90.53%), Query Frame = -2

Query:    8 FGAVKRCWS*CIMISQASSEHSVCFAVPEKEVKAVAEALQSRFRQALDNGRLSQVAVIPNCSILAAVGQKMASTPGVSATLFNALAKANINVRAIAQGCSEYNITVVVKREDCIKALRAVHSRFYLSRTTIAMGVIGPGLIGSTPLDQLRDQASVLKEEFNIDLRVMGILGSKSMLLSDVGIDLARWKEL 577
            F AVK   +  IMISQASSEHSVCFAVPEKEVKAV+EAL SRFRQAL  GRLSQ+ +IPNCSILAAVGQKMASTPGVSAT FNALAKANIN+RAIAQGCSE+NITVVVKREDCI+ALRAVHSRFYLSRTT+A+G+IGPGLIG T LDQ+RDQA+VLKEEF IDLRV+GI GS  ML+S+ GIDL+RW+EL
Sbjct:  423 FSAVKEVGANVIMISQASSEHSVCFAVPEKEVKAVSEALNSRFRQALAGGRLSQIEIIPNCSILAAVGQKMASTPGVSATFFNALAKANINIRAIAQGCSEFNITVVVKREDCIRALRAVHSRFYLSRTTLAVGIIGPGLIGGTLLDQIRDQAAVLKEEFKIDLRVIGITGSSKMLMSESGIDLSRWREL 612

[Close]

BLAST of HO064263 vs. TAIR peptide
Match: AT4G19710.2 (| Symbols: AK-HSDH II, AK-HSDH | aspartate kinase-homoserine dehydrogenase ii | chr4:10725229-10729536 FORWARD LENGTH=916)

HSP 1 Score: 305.064 bits (780), Expect = 2.379e-83
Identity = 153/190 (80.53%), Postives = 174/190 (91.58%), Query Frame = -2

Query:    8 FGAVKRCWS*CIMISQASSEHSVCFAVPEKEVKAVAEALQSRFRQALDNGRLSQVAVIPNCSILAAVGQKMASTPGVSATLFNALAKANINVRAIAQGCSEYNITVVVKREDCIKALRAVHSRFYLSRTTIAMGVIGPGLIGSTPLDQLRDQASVLKEEFNIDLRVMGILGSKSMLLSDVGIDLARWKEL 577
            FG VK   +  IMISQASSEHSVCFAVPEKEV AV+EAL+SRF +AL  GRLSQ+ VIPNCSILAAVGQKMASTPGVS TLF+ALAKANINVRAI+QGCSEYN+TVV+KRED +KALRAVHSRF+LSRTT+AMG++GPGLIG+T LDQLRDQA+VLK+EFNIDLRV+GI GSK MLLSD+GIDL+RW+EL
Sbjct:  428 FGCVKDVGANVIMISQASSEHSVCFAVPEKEVNAVSEALRSRFSEALQAGRLSQIEVIPNCSILAAVGQKMASTPGVSCTLFSALAKANINVRAISQGCSEYNVTVVIKREDSVKALRAVHSRFFLSRTTLAMGIVGPGLIGATLLDQLRDQAAVLKQEFNIDLRVLGITGSKKMLLSDIGIDLSRWREL 617

[Close]

BLAST of HO064263 vs. TAIR peptide
Match: AT4G19710.1 (| Symbols: AK-HSDH II, AK-HSDH | aspartate kinase-homoserine dehydrogenase ii | chr4:10725229-10729278 FORWARD LENGTH=859)

HSP 1 Score: 305.064 bits (780), Expect = 2.379e-83
Identity = 153/190 (80.53%), Postives = 174/190 (91.58%), Query Frame = -2

Query:    8 FGAVKRCWS*CIMISQASSEHSVCFAVPEKEVKAVAEALQSRFRQALDNGRLSQVAVIPNCSILAAVGQKMASTPGVSATLFNALAKANINVRAIAQGCSEYNITVVVKREDCIKALRAVHSRFYLSRTTIAMGVIGPGLIGSTPLDQLRDQASVLKEEFNIDLRVMGILGSKSMLLSDVGIDLARWKEL 577
            FG VK   +  IMISQASSEHSVCFAVPEKEV AV+EAL+SRF +AL  GRLSQ+ VIPNCSILAAVGQKMASTPGVS TLF+ALAKANINVRAI+QGCSEYN+TVV+KRED +KALRAVHSRF+LSRTT+AMG++GPGLIG+T LDQLRDQA+VLK+EFNIDLRV+GI GSK MLLSD+GIDL+RW+EL
Sbjct:  428 FGCVKDVGANVIMISQASSEHSVCFAVPEKEVNAVSEALRSRFSEALQAGRLSQIEVIPNCSILAAVGQKMASTPGVSCTLFSALAKANINVRAISQGCSEYNVTVVIKREDSVKALRAVHSRFFLSRTTLAMGIVGPGLIGATLLDQLRDQAAVLKQEFNIDLRVLGITGSKKMLLSDIGIDLSRWREL 617

The following BLAST results are available for this feature:

BLAST of HO064263 vs. SwissProt
BLAST of HO064263 vs. TrEMBL
BLAST of HO064263 vs. TAIR peptide

BLAST of HO064263 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Swissprot)
Total hits: 10


Match Name	E-value	Identity	Description
AKH1_ARATH	1.058e-82	81.58	Bifunctional aspartokinase/homoserine dehydrogenas...	[more]
AKH2_ARATH	2.357e-82	80.53	Bifunctional aspartokinase/homoserine dehydrogenas...	[more]
AKH_DAUCA	5.805e-81	78.65	Bifunctional aspartokinase/homoserine dehydrogenas...	[more]
AKH1_MAIZE	2.136e-75	75.13	Bifunctional aspartokinase/homoserine dehydrogenas...	[more]
AKH2_MAIZE	1.213e-70	71.58	Bifunctional aspartokinase/homoserine dehydrogenas...	[more]
AK1H_ECOLI	2.093e-30	39.68	Bifunctional aspartokinase/homoserine dehydrogenas...	[more]
AKH_HAEIN	7.446e-28	36.16	Bifunctional aspartokinase/homoserine dehydrogenas...	[more]
AK1H_SERMA	1.659e-27	37.57	Bifunctional aspartokinase/homoserine dehydrogenas...	[more]
AKH_BUCAI	5.900e-25	35.03	Bifunctional aspartokinase/homoserine dehydrogenas...	[more]
AKH_BUCAP	3.957e-21	29.21	Bifunctional aspartokinase/homoserine dehydrogenas...	[more]

BLAST of HO064263 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
O65027_SOYBN	3.049e-93	94.27	Aspartokinase-homoserine dehydrogenase (Fragment) ...	[more]
O63067_SOYBN	8.872e-93	93.75	Aspartokinase-homoserine dehydrogenase OS=Glycine ...	[more]
B9SHP2_RICCO	1.621e-86	88.42	Aspartate kinase, putative OS=Ricinus communis GN=...	[more]
D7TL78_VITVI	1.161e-84	85.26	Whole genome shotgun sequence of line PN40024, sca...	[more]
A5APD8_VITVI	1.161e-84	85.26	Putative uncharacterized protein OS=Vitis vinifera...	[more]
B9MWJ3_POPTR	2.861e-83	84.74	Predicted protein (Fragment) OS=Populus trichocarp...	[more]
B9IQ92_POPTR	4.880e-83	84.74	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
D7MG88_ARALY	2.678e-81	81.05	AK-HSDH/AK-HSDH II OS=Arabidopsis lyrata subsp. ly...	[more]
Q0WRP9_ARATH	3.497e-81	80.53	Aspartate kinase-homoserine dehydrogenase-like pro...	[more]
D7KK95_ARALY	3.866e-80	80.00	AK-HSDH I OS=Arabidopsis lyrata subsp. lyrata GN=A...	[more]

BLAST of HO064263 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide)
Total hits: 3


Match Name	E-value	Identity	Description
AT1G31230.1	1.068e-83	81.58	\| Symbols: AK-HSDH I, AK-HSDH \| aspartate kinase-h...	[more]
AT4G19710.2	2.379e-83	80.53	\| Symbols: AK-HSDH II, AK-HSDH \| aspartate kinase-...	[more]
AT4G19710.1	2.379e-83	80.53	\| Symbols: AK-HSDH II, AK-HSDH \| aspartate kinase-...	[more]

InterPro

Analysis Name: InterProScan analysis for Cicer arietinum unigene v1
Date Performed: 2010-12-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	SEG	seg	seg	coord: 5..16 score
None	No IPR available	PANTHER	PTHR21499	ASPARTATE KINASE	coord: 2..182 score: 1.1
None	No IPR available	PANTHER	PTHR21499:SF1	ASPARTATE KINASE	coord: 2..182 score: 1.1
None	No IPR available	SUPERFAMILY	51735	NAD(P)-binding Rossmann-fold domains	coord: 117..180 score: 1.8
None	No IPR available	SUPERFAMILY	55021	ACT-like	coord: 37..137 score: 2.4e-26coord: 2..45 score: 3.6
IPR002912	Amino acid-binding ACT	PFAM	PF01842	ACT	coord: 60..114 score: 2.9
IPR016040	NAD(P)-binding domain	GENE3D	3.40.50.720	no description	coord: 119..180 score: 1.3

Properties


Property Name	Value
Singlet in analysis	Cicer arietinum unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>HO064263 ID=HO064263; Name=HO064263; organism=Cicer arietinum; type=EST; length=689bp
CCTCTCGAAGTTCTTTCCACCTTGCTAAGTCAATACCCACATCACTGAGG
AGCATTGACTTTGAGCCAAGTATGCCCATTACACGCAAATCAATGTTAAA
TTCTTCTTTTAGAACTGAAGCCTGATCCCTTAGCTGATCAAGTGGTGTGC
TCCCAATTAATCCTGGTCCGATAACGCCCATTGCTATGGTGGTTCTTGAA
AGATAAAATCTGGAATGGACAGCCCGTAAAGCTTTTATACAATCCTCTCT
CTTAACAACAACAGTAATATTATACTCAGAACAACCTTGTGCTATAGCTC
GGACATTTATATTTGCCTTAGCCAATGCATTGAAAAGGGTGGCACTAACT
CCAGGAGTGCTTGCCATTTTCTGGCCAACAGCAGCCAAAATGCTACAATT
TGGGATAACTGCAACCTGAGAAAGACGCCCATTATCCAAAGCTTGGCGAA
ATCTTGATTGCAATGCCTCGGCAACAGCTTTTACTTCCTTCTCGGGCACA
GCAAAGCATACAGAATGCTCACTACTAGCCTGAGATATCATGATACATTA
GCTCCAACATCTTTTTACTGCGCCAAAATAGCACTGGCTGTACCTGGACA
CCGGCCATTCCAGTTCCTCAAATTTACAAGTGCCAAGTTGTCTATTGTTG
CAATCCTTGACAAATTTGCATGTTTGTCCTATCTCATTA

Annotated Terms

The following terms have been associated with this EST:

Vocabulary: **Biological Process**
Term	Definition
GO:0008152	metabolic process

Vocabulary: **Molecular Function**
Term	Definition
GO:0016597	amino acid binding
GO:0003824	catalytic activity
GO:0005488	binding

Vocabulary: **INTERPRO**
Term	Definition
IPR002912	ACT_dom
IPR016040	NAD(P)-bd_dom

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