HO067999

EST Overview


Name	HO067999
Unique Name	HO067999
Type	EST
Organism	Cicer arietinum (chickpea)
Sequence length	626

Libraries


Library Name	Type
LIBEST_026415 Chickpea drought stressed cDNA SSH library BULK2	cdna_library

Unigenes

This EST is part of the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
Cicer arietinum unigene v1	Cicer arietinum unigene v1	EST

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
Cicer arietinum unigene v1	2010-12-27
Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide	2011-02-03
KEGG analysis for Cicer arietinum unigene v1	2011-01-05
Homology Analysis: Cicer arietinum unigene v1 vs Swissprot	2010-12-28
Homology Analysis: Cicer arietinum unigene v1 vs Trembl	2010-12-28
InterProScan analysis for Cicer arietinum unigene v1	2010-12-29

Homology

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BLAST of HO067999 vs. SwissProt
Match: GALK1_ARATH (Galactokinase OS=Arabidopsis thaliana GN=GAL1 PE=2 SV=2)

HSP 1 Score: 253.447 bits (646), Expect = 6.763e-67
Identity = 123/172 (71.51%), Postives = 144/172 (83.72%), Query Frame = 2

Query:    2 PYTAEEIGDVTGEKLTSFLNINASYLEVIKAAKQYKLHQRAAHVYSEAKRVYAFKDVVSSNLSDEEMLKKLGDLMNESHYSCSNLYECSCPELEELVKICRDNGAFGARLTGAGWGGCAVALVKESIVPQFILNLKESYFQSRIDKGVIKKNDLGLYVFASKPSSGAAIFKL 517
            PYTAEEI  +  EKL S +N + + L V+ AA  +KLHQRAAHVYSEA+RV+ FKD V+SNLSDEE LKKLGDLMNESHYSCS LYECSCPELEELV++C++NGA GARLTGAGWGGCAVALVKE  V QFI  +KE Y++ R++KGV+KK D+ LY+FASKPSSGAAIF L
Sbjct:  325 PYTAEEIEKILEEKLPSIVNNDPTSLAVLNAATHFKLHQRAAHVYSEARRVHGFKDTVNSNLSDEEKLKKLGDLMNESHYSCSVLYECSCPELEELVQVCKENGALGARLTGAGWGGCAVALVKEFDVTQFIPAVKEKYYKKRVEKGVVKKEDMELYLFASKPSSGAAIFNL 496

[Close]

BLAST of HO067999 vs. SwissProt
Match: GALK2_RAT (N-acetylgalactosamine kinase OS=Rattus norvegicus GN=Galk2 PE=2 SV=1)

HSP 1 Score: 140.198 bits (352), Expect = 8.344e-33
Identity = 77/174 (44.25%), Postives = 107/174 (61.49%), Query Frame = 2

Query:    2 PYTAEEIGDVTGEKL----TSFLNINASYLEVIKAAKQYKLHQRAAHVYSEAKRVYAFKDVVSSNLSDEEMLKKLGDLMNESHYSCSNLYECSCPELEELVKICRDNGAFGARLTGAGWGGCAVALVKESIVPQFILNLKESYFQSRIDKGVIKKNDLGLYVFASKPSSGAAIF 511
            PY+ EEI    G  L    T  L+ N       +    +KL+QRA HVYSEA RV  FK V  +  + +  ++ LG+LMN+SH SC ++YECSCPEL++LV ICR  GA G+RLTGAGWGGC V+LV    +  F+ ++ E+Y+Q  + +   +K+ L    FA+KP  GA +F
Sbjct:  294 PYSREEICKCLGISLEELRTQILSPNT------QGELTFKLYQRAKHVYSEAARVLQFKQVCEA--APDNAVQLLGELMNQSHRSCRDMYECSCPELDQLVDICRKFGAKGSRLTGAGWGGCTVSLVPADTLSSFLASVHEAYYQGNMSRLAREKHSL----FATKPGGGALVF 455

[Close]

BLAST of HO067999 vs. SwissProt
Match: GALK2_MOUSE (N-acetylgalactosamine kinase OS=Mus musculus GN=Galk2 PE=1 SV=1)

HSP 1 Score: 139.043 bits (349), Expect = 1.859e-32
Identity = 77/175 (44.00%), Postives = 106/175 (60.57%), Query Frame = 2

Query:    2 PYTAEEIGDVTGEKL----TSFLNINASYLEVIKAAKQYKLHQRAAHVYSEAKRVYAFKDVVSSNLSDEEMLKKLGDLMNESHYSCSNLYECSCPELEELVKICRDNGAFGARLTGAGWGGCAVALVKESIVPQFILNLKESYFQSRIDKGVIKKNDLGLYVFASKPSSGAAIFK 514
            PY+ EEI    G  L    T  L  N       +    +KL+QRA HVYSEA RV  FK V     + +  ++ LG+LMN+SH SC ++YECSCPEL++LV ICR  GA G+RLTGAGWGGC V+LV   ++  F+ ++ E+Y+Q    +   +K+ L    FA+KP  GA +F+
Sbjct:  294 PYSREEICRCLGISLERLRTQILTPNT------QDELTFKLYQRAKHVYSEAARVLQFKQVCED--APDNAVQLLGELMNQSHRSCRDMYECSCPELDQLVDICRKFGAKGSRLTGAGWGGCTVSLVPADMLSSFLASVHEAYYQGNTSRLAQEKHSL----FATKPGGGALVFR 456

[Close]

BLAST of HO067999 vs. SwissProt
Match: GALK2_HUMAN (N-acetylgalactosamine kinase OS=Homo sapiens GN=GALK2 PE=1 SV=1)

HSP 1 Score: 138.272 bits (347), Expect = 3.171e-32
Identity = 77/173 (44.51%), Postives = 104/173 (60.12%), Query Frame = 2

Query:    2 PYTAEEIGDVTGEKL----TSFLNINASYLEVIKAAKQYKLHQRAAHVYSEAKRVYAFKDVVSSNLSDEEMLKKLGDLMNESHYSCSNLYECSCPELEELVKICRDNGAFGARLTGAGWGGCAVALVKESIVPQFILNLKESYFQSRIDKGVIKKNDLGLYVFASKPSSGAAI 508
            PY  EEI    G  L    T  L+ N   + +      +KL+QRA HVYSEA RV  FK +     + E M++ LG+LMN+SH SC ++YECSCPEL++LV ICR  GA G+RLTGAGWGGC V++V    +P F+ N+ ++Y+Q        +K  L    FA+KP  GA +
Sbjct:  294 PYNPEEICRCLGISLEELRTQILSPNTQDVLI------FKLYQRAKHVYSEAARVLQFKKICEE--APENMVQLLGELMNQSHMSCRDMYECSCPELDQLVDICRKFGAQGSRLTGAGWGGCTVSMVPADKLPSFLANVHKAYYQRSDGSLAPEKQSL----FATKPGGGALV 454

[Close]

BLAST of HO067999 vs. SwissProt
Match: GALK2_PONAB (N-acetylgalactosamine kinase OS=Pongo abelii GN=GALK2 PE=2 SV=1)

HSP 1 Score: 136.346 bits (342), Expect = 1.205e-31
Identity = 76/173 (43.93%), Postives = 103/173 (59.54%), Query Frame = 2

Query:    2 PYTAEEIGDVTGEKL----TSFLNINASYLEVIKAAKQYKLHQRAAHVYSEAKRVYAFKDVVSSNLSDEEMLKKLGDLMNESHYSCSNLYECSCPELEELVKICRDNGAFGARLTGAGWGGCAVALVKESIVPQFILNLKESYFQSRIDKGVIKKNDLGLYVFASKPSSGAAI 508
            PY  EEI    G  L    T  L+ N   + +      +KL+QRA HVYSEA RV  FK +     + E M++ LG+LMN+SH SC ++YECSCPEL++LV ICR  GA G+RLTGAGWGGC V++V    +P F+ N+ ++Y+         +K  L    FA+KP  GA +
Sbjct:  294 PYNPEEICRCLGISLEELRTQILSPNTQDVLI------FKLYQRAKHVYSEAARVLQFKKICEE--APENMVQLLGELMNQSHMSCRDMYECSCPELDQLVDICRKFGAQGSRLTGAGWGGCTVSIVPADKLPSFLANVHKAYYHRSDGSLAPEKQSL----FATKPGGGALV 454

[Close]

BLAST of HO067999 vs. SwissProt
Match: GALK_DICDI (Galactokinase OS=Dictyostelium discoideum GN=galK PE=3 SV=1)

HSP 1 Score: 127.872 bits (320), Expect = 4.285e-29
Identity = 65/180 (36.11%), Postives = 105/180 (58.33%), Query Frame = 2

Query:   32 TGEKLTSFLNINASYLE-------VIKAAKQYKLHQRAAHVYSEAKRVYAFKDVV--------------SSNLSDEEMLKKLGDLMNESHYSCSNLYECSCPELEELVKICRDNGAFGARLTGAGWGGCAVALVKESIVPQFILNLKESYFQSRIDKGVIKKNDLGLYVFASKPSSGAAI 508
            T E++ + L+I+   L        +   ++ ++L++RA HV++E +RVY F ++               +++ ++  ++++LG LMNESH SCS L+ECSC EL+ L KICR+NGA G+RLTGAGWGGC ++LV  S V  F+  +   Y+   ++   +K  +   Y F + P  GA I
Sbjct:  317 TREEVATILDISVEQLVKTYFPSGITVQSEHFELYKRARHVFTETQRVYKFSEICKQQSNFNNNNNNNNNNSSNNTNIIQELGKLMNESHESCSKLFECSCSELDSLTKICRENGALGSRLTGAGWGGCVISLVPNSKVDSFLDAIDTHYYSKFVNPEKLKNIEKSSYSFFTTPCKGACI 496

[Close]

BLAST of HO067999 vs. SwissProt
Match: GAL1_KLULA (Galactokinase OS=Kluyveromyces lactis GN=GAL1 PE=2 SV=2)

HSP 1 Score: 108.227 bits (269), Expect = 3.514e-23
Identity = 64/171 (37.43%), Postives = 91/171 (53.22%), Query Frame = 2

Query:    5 YTAEEIGDVTG-EKLTSFLNINASYLEVIKAAKQYKLHQRAAHVYSEAKRVYAFKDVVSSNLSDEEMLKKLGDLMNESHYSCSNLYECSCPELEELVKICRDNGAFGARLTGAGWGGCAVALVKESIVPQFILNLKESYFQSRIDKGVIKKNDLGLYVFASKPSSGAAIFK 514
            YT +   ++ G E ++ F  +  +   V    ++ KL QRA HVYSEA RV   K +      +    ++ G LMNES  SC  LYECSCPE + + +I   NG+FG+RLTGAGWGGC V L     V      L E Y+  R  +  +   +L   +  SKPS G+ +++
Sbjct:  339 YTLDHAVELCGCESISQFTELYLTNFPV--RFQRLKLFQRAKHVYSEALRV--LKALQLFQKGESNFFEEFGALMNESQESCDKLYECSCPETDSICEIALKNGSFGSRLTGAGWGGCTVHLCSTDTVDSVKSALTEQYYNLRFPE--LTAEELEDAIIISKPSLGSVLYE 503

[Close]

BLAST of HO067999 vs. SwissProt
Match: GAL1_YEAST (Galactokinase OS=Saccharomyces cerevisiae GN=GAL1 PE=1 SV=4)

HSP 1 Score: 106.686 bits (265), Expect = 1.022e-22
Identity = 58/142 (40.85%), Postives = 86/142 (60.56%), Query Frame = 2

Query:  107 KLHQRAAHVYSEAKRVYAFKDVVS--SNLSDEEMLKKLGDLMNESHYSCSNLYECSCPELEELVKICRDNGAFGARLTGAGWGGCAVALV---KESIVPQFILNLKESYFQSRIDKGVIKKNDLGLYVFASKPSSGAAIFKL 517
            KL+QRA HVYSE+ RV     +++  S  +DE+  K+ G LMNES  SC  LYECSCPE++++  I   NG++G+RLTGAGWGGC V LV       + +    L   +++ +  K  I   +L   +  SKP+ G+ +++L
Sbjct:  389 KLYQRAKHVYSESLRVLKAVKLMTTASFTADEDFFKQFGALMNESQASCDKLYECSCPEIDKICSIALSNGSYGSRLTGAGWGGCTVHLVPGGPNGNIEKVKEALANEFYKVKYPK--ITDAELENAIIVSKPALGSCLYEL 528

[Close]

BLAST of HO067999 vs. SwissProt
Match: GAL3_YEAST (Protein GAL3 OS=Saccharomyces cerevisiae GN=GAL3 PE=1 SV=2)

HSP 1 Score: 100.138 bits (248), Expect = 9.569e-21
Identity = 59/141 (41.84%), Postives = 79/141 (56.03%), Query Frame = 2

Query:  107 KLHQRAAHVYSEAKRVY-AFKDVVSSNL-SDEEMLKKLGDLMNESHYSCSNLYECSCPELEELVKICRDNGAFGARLTGAGWGGCAVALVKESI---VPQFILNLKESYFQSRIDKGVIKKNDLGLYVFASKPSSGAAIFK 514
            KL+QRA HVYSE+ RV  A K + S+   +DE+     G LMNES  SC  LYECSC E  ++  I   NG+FG+RLTGAGWGGC + LV       V Q    L E ++  R     +   +L   +  SKP+ G  +++
Sbjct:  381 KLYQRAKHVYSESLRVLKALKMMTSATFHTDEDFFTDFGRLMNESQASCDKLYECSCIETNQICSIALANGSFGSRLTGAGWGGCTIHLVPSGANGNVEQVRKALIEKFYNVRYPD--LTDEELKDAIIVSKPALGTCLYE 519

[Close]

BLAST of HO067999 vs. SwissProt
Match: GAL1_CANPA (Galactokinase OS=Candida parapsilosis GN=GAL1 PE=3 SV=1)

HSP 1 Score: 88.9669 bits (219), Expect = 2.206e-17
Identity = 59/172 (34.30%), Postives = 91/172 (52.91%), Query Frame = 2

Query:    5 YTAEEIGDVTGEKLTSFLNINASYLEVIKAAKQY-KLHQRAAHVYSEAKRVYAFKDVVSSNLSDEEMLKKLGDLMNESHYSCSNLYECSCPELEELVKICRDNGAFGARLTGAGWGGCAVALVKESIVPQFILNLKESYFQSRIDKGVIKKNDLGLYVFASKPSSGAAIFKL 517
            YT E+  +  G     F   ++ YL+ I       KL++RA HVY E+ RV     ++S+ +   + L+  G LMNES +    L E S P+L E+  I   NGA+G+R+TGAGWGG  V L     +P+   +L E+Y++       I   ++   V  SKP++G+ + KL
Sbjct:  331 YTVEQCCEEMGLTPEEF---HSRYLKKIPVKFDVLKLYERALHVYRESLRVLKTLQLLSTVVDASQFLQTFGSLMNESQHDLDILNESSNPKLNEICSIALANGAYGSRVTGAGWGGSIVHLTTTENLPKLTKSL-EAYYKREFPG--ITDEEIREAVIDSKPATGSCLVKL 496

[Close]

BLAST of HO067999 vs. TrEMBL
Match: Q2PEV9_TRIPR (Putative galactose kinase OS=Trifolium pratense PE=2 SV=1)

HSP 1 Score: 320.087 bits (819), Expect = 8.383e-86
Identity = 159/171 (92.98%), Postives = 164/171 (95.91%), Query Frame = 2

Query:    2 PYTAEEIGDVTGEKLTSFLNINASYLEVIKAAKQYKLHQRAAHVYSEAKRVYAFKDVVSSNLSDEEMLKKLGDLMNESHYSCSNLYECSCPELEELVKICRDNGAFGARLTGAGWGGCAVALVKESIVPQFILNLKESYFQSRIDKGVIKKNDLGLYVFASKPSSGAAIFK 514
            PYTAEEI  VTGEKLTSFLNINASYL+VIKAAKQYKLHQRAAHVYSEAKRVYAFKDVVSSNLSDEE LKKLGDLMNESHYSCSNLYECSCPELEEL K+ RDNGAFGARLTGAGWGGCAVALVKESIVPQFILNLKE Y+Q RIDKGVIKK+DLGLYVFASKPSSG+AIFK
Sbjct:  325 PYTAEEIEAVTGEKLTSFLNINASYLDVIKAAKQYKLHQRAAHVYSEAKRVYAFKDVVSSNLSDEEKLKKLGDLMNESHYSCSNLYECSCPELEELTKVSRDNGAFGARLTGAGWGGCAVALVKESIVPQFILNLKEHYYQPRIDKGVIKKDDLGLYVFASKPSSGSAIFK 495

[Close]

BLAST of HO067999 vs. TrEMBL
Match: Q5ZP80_PEA (Galactokinase OS=Pisum sativum GN=galK PE=2 SV=1)

HSP 1 Score: 319.316 bits (817), Expect = 1.430e-85
Identity = 158/171 (92.40%), Postives = 164/171 (95.91%), Query Frame = 2

Query:    2 PYTAEEIGDVTGEKLTSFLNINASYLEVIKAAKQYKLHQRAAHVYSEAKRVYAFKDVVSSNLSDEEMLKKLGDLMNESHYSCSNLYECSCPELEELVKICRDNGAFGARLTGAGWGGCAVALVKESIVPQFILNLKESYFQSRIDKGVIKKNDLGLYVFASKPSSGAAIFK 514
            PYTAEEI ++TGE LTSFLNINASYLEVIKAAKQYKLHQRAAHVYSEAKRVYAFKDVVSSNLSDEE L KLG+LMNESHYSCSNLYECSCPELEEL KI RDNGAFGARLTGAGWGGCAVALVKE+IVPQFILNLKE Y+QSRIDKGVIKKNDLGLYVFASKPSSG+AIFK
Sbjct:  326 PYTAEEIENITGENLTSFLNINASYLEVIKAAKQYKLHQRAAHVYSEAKRVYAFKDVVSSNLSDEEKLNKLGELMNESHYSCSNLYECSCPELEELTKISRDNGAFGARLTGAGWGGCAVALVKENIVPQFILNLKEYYYQSRIDKGVIKKNDLGLYVFASKPSSGSAIFK 496

[Close]

BLAST of HO067999 vs. TrEMBL
Match: B9HX46_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_723945 PE=3 SV=1)

HSP 1 Score: 282.722 bits (722), Expect = 1.483e-74
Identity = 139/171 (81.29%), Postives = 153/171 (89.47%), Query Frame = 2

Query:    2 PYTAEEIGDVTGEKLTSFLNINASYLEVIKAAKQYKLHQRAAHVYSEAKRVYAFKDVVSSNLSDEEMLKKLGDLMNESHYSCSNLYECSCPELEELVKICRDNGAFGARLTGAGWGGCAVALVKESIVPQFILNLKESYFQSRIDKGVIKKNDLGLYVFASKPSSGAAIFK 514
            PYTAEEI ++TGE L S    + S L+V+KAA+ YKLHQRAAHVYSEAKRV+AFKD VSS+LSDE+ LKKLG+LMNESHYSCS LYECSCPELEELVKICRD+ A GARLTGAGWGGCAVALVKE IVPQFILNLKE ++QSRIDKGVI KNDLGLYVFASKPSSGAAIF+
Sbjct:  325 PYTAEEIEEITGESLRSIFKNSPSSLDVLKAAEHYKLHQRAAHVYSEAKRVHAFKDTVSSDLSDEDKLKKLGELMNESHYSCSVLYECSCPELEELVKICRDSDALGARLTGAGWGGCAVALVKEPIVPQFILNLKEKFYQSRIDKGVISKNDLGLYVFASKPSSGAAIFR 495

[Close]

BLAST of HO067999 vs. TrEMBL
Match: B9RZT4_RICCO (Galactokinase, putative OS=Ricinus communis GN=RCOM_1001220 PE=3 SV=1)

HSP 1 Score: 281.567 bits (719), Expect = 3.304e-74
Identity = 138/171 (80.70%), Postives = 155/171 (90.64%), Query Frame = 2

Query:    2 PYTAEEIGDVTGEKLTSFLNINASYLEVIKAAKQYKLHQRAAHVYSEAKRVYAFKDVVSSNLSDEEMLKKLGDLMNESHYSCSNLYECSCPELEELVKICRDNGAFGARLTGAGWGGCAVALVKESIVPQFILNLKESYFQSRIDKGVIKKNDLGLYVFASKPSSGAAIFK 514
            PY+AEEI  +T E L+S  + + + L+V+KAAK +KLHQRAAHVYSEAKRV+AFKD VSS LSDE+ LKKLGDLMNESHYSCS LYECSCPELEELVK+CR++GA GARLTGAGWGGCAVALVKE+IVPQFILNLKE +FQSRIDKGVIKK+DLGLYVFASKPSSGAAIFK
Sbjct:  328 PYSAEEIEKITEEDLSSIFSNSPTSLDVLKAAKHFKLHQRAAHVYSEAKRVHAFKDTVSSKLSDEDKLKKLGDLMNESHYSCSILYECSCPELEELVKVCREHGALGARLTGAGWGGCAVALVKEAIVPQFILNLKEKFFQSRIDKGVIKKSDLGLYVFASKPSSGAAIFK 498

[Close]

BLAST of HO067999 vs. TrEMBL
Match: B9HIL5_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_820551 PE=3 SV=1)

HSP 1 Score: 279.641 bits (714), Expect = 1.256e-73
Identity = 138/171 (80.70%), Postives = 150/171 (87.72%), Query Frame = 2

Query:    2 PYTAEEIGDVTGEKLTSFLNINASYLEVIKAAKQYKLHQRAAHVYSEAKRVYAFKDVVSSNLSDEEMLKKLGDLMNESHYSCSNLYECSCPELEELVKICRDNGAFGARLTGAGWGGCAVALVKESIVPQFILNLKESYFQSRIDKGVIKKNDLGLYVFASKPSSGAAIFK 514
            PYT EEI  +TGE L S    + S L+V+KAAK +KLH RAAHVYSEAKRV+AFKD VSS+LSDE+ LKKLG+LMNESHYSCS LYECSCPELEELVKICRDN A GARLTGAGWGGCAVALVKE+ VPQFILNLKE ++QSRIDKGVI KNDLGLYVFASKPSSGAAIFK
Sbjct:  325 PYTTEEIEAITGESLQSIFKNSPSSLDVLKAAKHFKLHHRAAHVYSEAKRVHAFKDAVSSDLSDEDKLKKLGELMNESHYSCSVLYECSCPELEELVKICRDNDALGARLTGAGWGGCAVALVKEATVPQFILNLKEKFYQSRIDKGVISKNDLGLYVFASKPSSGAAIFK 495

[Close]

BLAST of HO067999 vs. TrEMBL
Match: C5WU13_SORBI (Putative uncharacterized protein Sb01g002480 OS=Sorghum bicolor GN=Sb01g002480 PE=3 SV=1)

HSP 1 Score: 275.018 bits (702), Expect = 3.093e-72
Identity = 135/172 (78.49%), Postives = 150/172 (87.21%), Query Frame = 2

Query:    2 PYTAEEIGDVTGEKLTSFLNINASYLEVIKAAKQYKLHQRAAHVYSEAKRVYAFKDVVSSNLSDEEMLKKLGDLMNESHYSCSNLYECSCPELEELVKICRDNGAFGARLTGAGWGGCAVALVKESIVPQFILNLKESYFQSRIDKGVIKKNDLGLYVFASKPSSGAAIFKL 517
            PYTAEEI  +TGE LTS    + + L+VIKAAK YKL QRA HVYSEA+RVYAF+D VSS LS+E+ LKKLGDLMNESHYSCS LYECSCPELEELVK+CRDNGA GARLTGAGWGGCAVALVKE IVPQFILNLKE Y++SRI++GVI++ DLGLYVFASKPSSGAAI  L
Sbjct:  331 PYTAEEIEKITGESLTSVFQSSQTSLDVIKAAKHYKLFQRATHVYSEARRVYAFRDTVSSKLSEEDKLKKLGDLMNESHYSCSVLYECSCPELEELVKVCRDNGALGARLTGAGWGGCAVALVKEPIVPQFILNLKEMYYKSRIERGVIQQGDLGLYVFASKPSSGAAILTL 502

[Close]

BLAST of HO067999 vs. TrEMBL
Match: C4JAI6_MAIZE (Putative uncharacterized protein OS=Zea mays PE=2 SV=1)

HSP 1 Score: 270.011 bits (689), Expect = 9.948e-71
Identity = 134/172 (77.91%), Postives = 148/172 (86.05%), Query Frame = 2

Query:    2 PYTAEEIGDVTGEKLTSFLNINASYLEVIKAAKQYKLHQRAAHVYSEAKRVYAFKDVVSSNLSDEEMLKKLGDLMNESHYSCSNLYECSCPELEELVKICRDNGAFGARLTGAGWGGCAVALVKESIVPQFILNLKESYFQSRIDKGVIKKNDLGLYVFASKPSSGAAIFKL 517
            PYTA EI  +TGE LTS    + + L+VIKAAK YKL QRA HVYSEA+RVYAF+D VSS LS+E  LKKLGDLMNESHYSCS LYECSCPELEELVK+CRDNGA GARLTGAGWGGCAVALVKE IVPQFIL LKE Y++SRID+GVIK+ D+GLYVFASKPSSGAAI +L
Sbjct:   44 PYTAGEIEKITGEGLTSVFQGSQTSLDVIKAAKHYKLFQRATHVYSEARRVYAFRDTVSSKLSEEGKLKKLGDLMNESHYSCSVLYECSCPELEELVKVCRDNGALGARLTGAGWGGCAVALVKEPIVPQFILKLKEMYYKSRIDRGVIKQGDVGLYVFASKPSSGAAILRL 215

[Close]

BLAST of HO067999 vs. TrEMBL
Match: C0HGF4_MAIZE (Putative uncharacterized protein OS=Zea mays PE=2 SV=1)

HSP 1 Score: 270.011 bits (689), Expect = 9.948e-71
Identity = 134/172 (77.91%), Postives = 148/172 (86.05%), Query Frame = 2

Query:    2 PYTAEEIGDVTGEKLTSFLNINASYLEVIKAAKQYKLHQRAAHVYSEAKRVYAFKDVVSSNLSDEEMLKKLGDLMNESHYSCSNLYECSCPELEELVKICRDNGAFGARLTGAGWGGCAVALVKESIVPQFILNLKESYFQSRIDKGVIKKNDLGLYVFASKPSSGAAIFKL 517
            PYTA EI  +TGE LTS    + + L+VIKAAK YKL QRA HVYSEA+RVYAF+D VSS LS+E  LKKLGDLMNESHYSCS LYECSCPELEELVK+CRDNGA GARLTGAGWGGCAVALVKE IVPQFIL LKE Y++SRID+GVIK+ D+GLYVFASKPSSGAAI +L
Sbjct:  333 PYTAGEIEKITGEGLTSVFQGSQTSLDVIKAAKHYKLFQRATHVYSEARRVYAFRDTVSSKLSEEGKLKKLGDLMNESHYSCSVLYECSCPELEELVKVCRDNGALGARLTGAGWGGCAVALVKEPIVPQFILKLKEMYYKSRIDRGVIKQGDVGLYVFASKPSSGAAILRL 504

[Close]

BLAST of HO067999 vs. TrEMBL
Match: Q7Y152_ORYSJ (Galactokinase, putative, expressed OS=Oryza sativa subsp. japonica GN=OSJNBa0078D06.32 PE=3 SV=1)

HSP 1 Score: 269.626 bits (688), Expect = 1.299e-70
Identity = 131/171 (76.61%), Postives = 149/171 (87.13%), Query Frame = 2

Query:    5 YTAEEIGDVTGEKLTSFLNINASYLEVIKAAKQYKLHQRAAHVYSEAKRVYAFKDVVSSNLSDEEMLKKLGDLMNESHYSCSNLYECSCPELEELVKICRDNGAFGARLTGAGWGGCAVALVKESIVPQFILNLKESYFQSRIDKGVIKKNDLGLYVFASKPSSGAAIFKL 517
            YT EEI  +TG+ LTS    + + L+V++AAK +KL QRA HVYSEA+RVYAF+D V S LS E+ML+KLGDLMNESHYSCS LYECSCPELEELVK+CRDNGA GARLTGAGWGGCAVALVKE IVPQFILNLKE+Y++SRID+GVI + DLGLYVFASKPSSGAAIFKL
Sbjct:  336 YTTEEIEKITGQSLTSIFQSSQTSLDVLRAAKHFKLFQRAFHVYSEARRVYAFRDTVLSKLSAEDMLQKLGDLMNESHYSCSVLYECSCPELEELVKVCRDNGALGARLTGAGWGGCAVALVKEGIVPQFILNLKETYYKSRIDRGVINQKDLGLYVFASKPSSGAAIFKL 506

[Close]

BLAST of HO067999 vs. TrEMBL
Match: B6V3B9_CUCME (Galactokinase OS=Cucumis melo PE=2 SV=1)

HSP 1 Score: 269.626 bits (688), Expect = 1.299e-70
Identity = 131/171 (76.61%), Postives = 154/171 (90.06%), Query Frame = 2

Query:    2 PYTAEEIGDVTGEKLTSFLNINASYLEVIKAAKQYKLHQRAAHVYSEAKRVYAFKDVVSSNLSDEEMLKKLGDLMNESHYSCSNLYECSCPELEELVKICRDNGAFGARLTGAGWGGCAVALVKESIVPQFILNLKESYFQSRIDKGVIKKNDLGLYVFASKPSSGAAIFK 514
            PYTAEEI  +T + L S L  + + L+V+KAAK +KL+QRA+HVYSEA+RVYAFKD VSS+LS+E+ LKKLGDLMN+SHYSCS LYECSCPELEELVKICRDN A GARLTGAGWGGCAVALVKE+IVPQFI NLKES+++SRI++GVIKK+D+GLYVFASKPSSGAAIF+
Sbjct:  328 PYTAEEIEQITVDNLPSVLGNSPTSLDVLKAAKHFKLYQRASHVYSEARRVYAFKDAVSSSLSEEDKLKKLGDLMNDSHYSCSVLYECSCPELEELVKICRDNDALGARLTGAGWGGCAVALVKEAIVPQFIHNLKESFYKSRIERGVIKKDDIGLYVFASKPSSGAAIFQ 498

[Close]

BLAST of HO067999 vs. TAIR peptide
Match: AT3G06580.1 (| Symbols: GAL1, GALK | Mevalonate/galactokinase family protein | chr3:2049141-2051867 REVERSE LENGTH=496)

HSP 1 Score: 253.447 bits (646), Expect = 6.991e-68
Identity = 123/172 (71.51%), Postives = 144/172 (83.72%), Query Frame = 2

Query:    2 PYTAEEIGDVTGEKLTSFLNINASYLEVIKAAKQYKLHQRAAHVYSEAKRVYAFKDVVSSNLSDEEMLKKLGDLMNESHYSCSNLYECSCPELEELVKICRDNGAFGARLTGAGWGGCAVALVKESIVPQFILNLKESYFQSRIDKGVIKKNDLGLYVFASKPSSGAAIFKL 517
            PYTAEEI  +  EKL S +N + + L V+ AA  +KLHQRAAHVYSEA+RV+ FKD V+SNLSDEE LKKLGDLMNESHYSCS LYECSCPELEELV++C++NGA GARLTGAGWGGCAVALVKE  V QFI  +KE Y++ R++KGV+KK D+ LY+FASKPSSGAAIF L
Sbjct:  325 PYTAEEIEKILEEKLPSIVNNDPTSLAVLNAATHFKLHQRAAHVYSEARRVHGFKDTVNSNLSDEEKLKKLGDLMNESHYSCSVLYECSCPELEELVQVCKENGALGARLTGAGWGGCAVALVKEFDVTQFIPAVKEKYYKKRVEKGVVKKEDMELYLFASKPSSGAAIFNL 496

[Close]

BLAST of HO067999 vs. TAIR peptide
Match: AT3G10700.1 (| Symbols: GalAK | galacturonic acid kinase | chr3:3346789-3350863 REVERSE LENGTH=424)

HSP 1 Score: 51.6026 bits (122), Expect = 4.035e-7
Identity = 37/121 (30.58%), Postives = 57/121 (47.11%), Query Frame = 2

Query:   59 NINASYLEVIKAAKQYKLHQRAAHVYSEAKRVYAFKDVVSSNLSDEEMLKKLGDLMNESHYSCSNLYECSCPELEELVKIC-RDNGAFGARLTGAGWGGCAVALVKESIVPQFILNLKESY 418
            N+  +  E  K   +  L +RA H +SE  RV   ++  +S       L++ G L++ S  S    YEC    L +L KI  +  G +GAR +GAG+ GC +A V           +K+ Y
Sbjct:  279 NVEHAVYEAHKHELKPVLAKRAEHYFSENMRVIKGREAWASG-----NLEEFGKLISASGLSSIENYECGAEPLIQLYKILLKAPGVYGARFSGAGFRGCCLAFVDAEKAEAAASYVKDEY 394

The following BLAST results are available for this feature:

BLAST of HO067999 vs. SwissProt
BLAST of HO067999 vs. TrEMBL
BLAST of HO067999 vs. TAIR peptide

BLAST of HO067999 vs. SwissProt
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Swissprot)
Total hits: 10


Match Name	E-value	Identity	Description
GALK1_ARATH	6.763e-67	71.51	Galactokinase OS=Arabidopsis thaliana GN=GAL1 PE=2...	[more]
GALK2_RAT	8.344e-33	44.25	N-acetylgalactosamine kinase OS=Rattus norvegicus ...	[more]
GALK2_MOUSE	1.859e-32	44.00	N-acetylgalactosamine kinase OS=Mus musculus GN=Ga...	[more]
GALK2_HUMAN	3.171e-32	44.51	N-acetylgalactosamine kinase OS=Homo sapiens GN=GA...	[more]
GALK2_PONAB	1.205e-31	43.93	N-acetylgalactosamine kinase OS=Pongo abelii GN=GA...	[more]
GALK_DICDI	4.285e-29	36.11	Galactokinase OS=Dictyostelium discoideum GN=galK ...	[more]
GAL1_KLULA	3.514e-23	37.43	Galactokinase OS=Kluyveromyces lactis GN=GAL1 PE=2...	[more]
GAL1_YEAST	1.022e-22	40.85	Galactokinase OS=Saccharomyces cerevisiae GN=GAL1 ...	[more]
GAL3_YEAST	9.569e-21	41.84	Protein GAL3 OS=Saccharomyces cerevisiae GN=GAL3 P...	[more]
GAL1_CANPA	2.206e-17	34.30	Galactokinase OS=Candida parapsilosis GN=GAL1 PE=3...	[more]

BLAST of HO067999 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Trembl)
Total hits: 10


Match Name	E-value	Identity	Description
Q2PEV9_TRIPR	8.383e-86	92.98	Putative galactose kinase OS=Trifolium pratense PE...	[more]
Q5ZP80_PEA	1.430e-85	92.40	Galactokinase OS=Pisum sativum GN=galK PE=2 SV=1	[more]
B9HX46_POPTR	1.483e-74	81.29	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
B9RZT4_RICCO	3.304e-74	80.70	Galactokinase, putative OS=Ricinus communis GN=RCO...	[more]
B9HIL5_POPTR	1.256e-73	80.70	Predicted protein OS=Populus trichocarpa GN=POPTRD...	[more]
C5WU13_SORBI	3.093e-72	78.49	Putative uncharacterized protein Sb01g002480 OS=So...	[more]
C4JAI6_MAIZE	9.948e-71	77.91	Putative uncharacterized protein OS=Zea mays PE=2 ...	[more]
C0HGF4_MAIZE	9.948e-71	77.91	Putative uncharacterized protein OS=Zea mays PE=2 ...	[more]
Q7Y152_ORYSJ	1.299e-70	76.61	Galactokinase, putative, expressed OS=Oryza sativa...	[more]
B6V3B9_CUCME	1.299e-70	76.61	Galactokinase OS=Cucumis melo PE=2 SV=1	[more]

BLAST of HO067999 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide)
Total hits: 2


Match Name	E-value	Identity	Description
AT3G06580.1	6.991e-68	71.51	\| Symbols: GAL1, GALK \| Mevalonate/galactokinase f...	[more]
AT3G10700.1	4.035e-7	30.58	\| Symbols: GalAK \| galacturonic acid kinase \| chr3...	[more]

InterPro

Analysis Name: InterProScan analysis for Cicer arietinum unigene v1
Date Performed: 2010-12-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR013750	GHMP kinase, C-terminal	PFAM	PF08544	GHMP_kinases_C	coord: 68..139 score: 1.8
None	No IPR available	GENE3D	3.30.70.890	no description	coord: 21..167 score: 6.8
None	No IPR available	PANTHER	PTHR10457	MEVALONATE KINASE/GALACTOKINASE	coord: 1..172 score: 5.3
None	No IPR available	PANTHER	PTHR10457:SF7	GALACTOKINASE 2	coord: 1..172 score: 5.3
None	No IPR available	SUPERFAMILY	55060	GHMP Kinase, C-terminal domain	coord: 25..170 score: 2
None	No IPR available	SEG	seg	seg	coord: 1..18 score
None	No IPR available	SEG	seg	seg	coord: 46..60 score

Properties


Property Name	Value
Singlet in analysis	Cicer arietinum unigene v1

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>HO067999 ID=HO067999; Name=HO067999; organism=Cicer arietinum; type=EST; length=626bp
ACCATATACGGCTGAAGAAATTGGAGATGTTACTGGGGAAAAGCTGACTT
CGTTTTTGAATATTAATGCATCTTACTTGGAAGTCATAAAAGCTGCAAAG
CAATACAAGTTACACCAGAGAGCTGCACATGTGTATTCAGAAGCCAAGAG
GGTATATGCTTTCAAGGATGTTGTATCATCAAATCTAAGCGATGAGGAGA
TGCTAAAGAAACTTGGTGACCTTATGAACGAGAGTCATTATAGCTGCAGC
AATTTATATGAATGCAGCTGTCCGGAGTTGGAAGAACTAGTAAAGATTTG
TCGCGACAATGGTGCTTTTGGGGCAAGGCTTACTGGAGCTGGATGGGGTG
GTTGTGCCGTTGCTTTGGTGAAAGAGAGCATAGTCCCACAATTCATCCTT
AATTTGAAGGAATCTTACTTCCAATCTAGGATAGACAAAGGCGTCATTAA
GAAGAATGATCTTGGTCTTTATGTATTTGCTTCGAAGCCATCAAGTGGTG
CTGCCATCTTCAAGCTTTAGAATATTTCCTTCAAGGTTGTTAAACTCGAG
TGGTAACTCATAAACTCGTCTGATACCACGAGTTAAGGTCATGATTTCGA
GTCAACTCGGTTGTGGAAACGAAATA

Annotated Terms

The following terms have been associated with this EST:

Vocabulary: **INTERPRO**
Term	Definition
IPR013750	GHMP_kinase_C_dom

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