HO066208

EST Overview
NameHO066208
Unique NameHO066208
TypeEST
OrganismCicer arietinum (chickpea)
Sequence length321
Libraries
Library NameType
LIBEST_026412 Chickpea drought stressed cDNA SSH library AS1cdna_library
Unigenes
This EST is part of the following unigenes:
Unigene NameAnalysis NameSequence type in Unigene
Cicer arietinum unigene v1Cicer arietinum unigene v1EST
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
Cicer arietinum unigene v12010-12-27
Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide2011-02-03
Homology Analysis: Cicer arietinum unigene v1 vs Trembl2010-12-28
InterProScan analysis for Cicer arietinum unigene v12010-12-29
Homology
[Close]
BLAST of HO066208 vs. TrEMBL
Match: C6TER0_SOYBN (Putative uncharacterized protein OS=Glycine max PE=2 SV=1)

HSP 1 Score: 130.183 bits (326), Expect = 6.639e-29
Identity = 60/88 (68.18%), Postives = 67/88 (76.14%), Query Frame = 2 
Query:   26 MDFMCHCSNFLLPPDNPNAAXXXXXXXXXXXXLTYALVVLNQHLPKFAPLLWDHAHIRVCADGGANRVYDEVPLFFPQLNPSHVRTRF 289
            M+ MCHCS+FLLPP+NPN              L YALVVLNQ LP+FAPLLWDHA +RVCADGGANRVYDE+PLFFP   PSHVRTR+
Sbjct:    1 MELMCHCSSFLLPPENPNTTCSLS--------LKYALVVLNQSLPRFAPLLWDHAQVRVCADGGANRVYDEMPLFFPHQQPSHVRTRY 80
[Close]
BLAST of HO066208 vs. TrEMBL
Match: Q3EBG0_ARATH (Putative uncharacterized protein At2g44750.2 OS=Arabidopsis thaliana GN=At2g44750 PE=4 SV=1)

HSP 1 Score: 97.0561 bits (240), Expect = 6.228e-19
Identity = 47/88 (53.41%), Postives = 58/88 (65.91%), Query Frame = 2 
Query:   26 MDFMCHCSNFLLPPDNPNAAXXXXXXXXXXXXLTYALVVLNQHLPKFAPLLWDHAHIRVCADGGANRVYDEVPLFFPQLNPSHVRTRF 289
            MD M H S+FLLP D                   YALVVLNQ+LP+F PLLW+HA +R+CADGGANR+YDE+PLFFP  +P  +R R+
Sbjct:    5 MDVMIHSSSFLLPCDETCGTR-------------YALVVLNQNLPRFTPLLWEHAKLRLCADGGANRIYDELPLFFPHEDPFVIRNRY 79
[Close]
BLAST of HO066208 vs. TrEMBL
Match: O80514_ARATH (Putative thiamin pyrophosphokinase OS=Arabidopsis thaliana GN=At2g44750/F16B22.24 PE=2 SV=2)

HSP 1 Score: 97.0561 bits (240), Expect = 6.228e-19
Identity = 47/88 (53.41%), Postives = 58/88 (65.91%), Query Frame = 2 
Query:   26 MDFMCHCSNFLLPPDNPNAAXXXXXXXXXXXXLTYALVVLNQHLPKFAPLLWDHAHIRVCADGGANRVYDEVPLFFPQLNPSHVRTRF 289
            MD M H S+FLLP D                   YALVVLNQ+LP+F PLLW+HA +R+CADGGANR+YDE+PLFFP  +P  +R R+
Sbjct:    5 MDVMIHSSSFLLPCDETCGTR-------------YALVVLNQNLPRFTPLLWEHAKLRLCADGGANRIYDELPLFFPHEDPFVIRNRY 79
[Close]
BLAST of HO066208 vs. TrEMBL
Match: B9GS93_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_816675 PE=4 SV=1)

HSP 1 Score: 96.6709 bits (239), Expect = 8.134e-19
Identity = 47/88 (53.41%), Postives = 61/88 (69.32%), Query Frame = 2 
Query:   26 MDFMCHCSNFLLPPDNPNAAXXXXXXXXXXXXLTYALVVLNQHLPKFAPLLWDHAHIRVCADGGANRVYDEVPLFFPQLNPSHVRTRF 289
            MD M H S FLLP ++ +++            +TYALVVLNQ LP+F P+LWDHA +RVCADGGANRV+DE+PL FP+ +   VR R+
Sbjct:    1 MDVMTHSSTFLLPGNDHSSS------------VTYALVVLNQRLPRFTPILWDHAQVRVCADGGANRVFDEMPLLFPRDDALDVRHRY 76
[Close]
BLAST of HO066208 vs. TrEMBL
Match: B9SAN6_RICCO (Thiamin pyrophosphokinase, putative OS=Ricinus communis GN=RCOM_1177290 PE=4 SV=1)

HSP 1 Score: 96.2857 bits (238), Expect = 1.062e-18
Identity = 49/88 (55.68%), Postives = 56/88 (63.64%), Query Frame = 2 
Query:   26 MDFMCHCSNFLLPPDNPNAAXXXXXXXXXXXXLTYALVVLNQHLPKFAPLLWDHAHIRVCADGGANRVYDEVPLFFPQLNPSHVRTRF 289
            M+ M H S FLLP  + +              L YAL+VLNQHLPKF PL+W HA IRVCADGGANRVYDE+PL FP  N   VR R+
Sbjct:    1 MEVMTHSSAFLLPTISDD----------HHPSLNYALIVLNQHLPKFTPLIWKHAKIRVCADGGANRVYDEMPLLFPHENALDVRQRY 78
[Close]
BLAST of HO066208 vs. TrEMBL
Match: Q9SRY1_ARATH (F22D16.12 protein OS=Arabidopsis thaliana GN=At1g02880 PE=2 SV=1)

HSP 1 Score: 95.1301 bits (235), Expect = 2.367e-18
Identity = 46/88 (52.27%), Postives = 57/88 (64.77%), Query Frame = 2 
Query:   26 MDFMCHCSNFLLPPDNPNAAXXXXXXXXXXXXLTYALVVLNQHLPKFAPLLWDHAHIRVCADGGANRVYDEVPLFFPQLNPSHVRTRF 289
            MD M H S+FLLP D  +                YALVVLNQ LP+F PLLW+HA +R+CADGGANR+YDE+PLFFP  +   +R R+
Sbjct:    1 MDVMIHSSSFLLPCDETSTGTR------------YALVVLNQSLPRFTPLLWEHAKLRLCADGGANRIYDELPLFFPNEDALAIRNRY 76
[Close]
BLAST of HO066208 vs. TrEMBL
Match: Q3EDJ9_ARATH (AT1G02880 protein OS=Arabidopsis thaliana GN=AT1G02880 PE=2 SV=1)

HSP 1 Score: 95.1301 bits (235), Expect = 2.367e-18
Identity = 46/88 (52.27%), Postives = 57/88 (64.77%), Query Frame = 2 
Query:   26 MDFMCHCSNFLLPPDNPNAAXXXXXXXXXXXXLTYALVVLNQHLPKFAPLLWDHAHIRVCADGGANRVYDEVPLFFPQLNPSHVRTRF 289
            MD M H S+FLLP D  +                YALVVLNQ LP+F PLLW+HA +R+CADGGANR+YDE+PLFFP  +   +R R+
Sbjct:    4 MDVMIHSSSFLLPCDETSTGTR------------YALVVLNQSLPRFTPLLWEHAKLRLCADGGANRIYDELPLFFPNEDALAIRNRY 79
[Close]
BLAST of HO066208 vs. TrEMBL
Match: D7KBD4_ARALY (Putative uncharacterized protein OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_470256 PE=4 SV=1)

HSP 1 Score: 94.3597 bits (233), Expect = 4.037e-18
Identity = 47/88 (53.41%), Postives = 58/88 (65.91%), Query Frame = 2 
Query:   26 MDFMCHCSNFLLPPDNPNAAXXXXXXXXXXXXLTYALVVLNQHLPKFAPLLWDHAHIRVCADGGANRVYDEVPLFFPQLNPSHVRTRF 289
            MD M H S+FLLP  NP                 YAL+VLNQ LP+FAPLLW+HA +R+CADGGANR+YDE+PLFFP  +   +R R+
Sbjct:    1 MDVMLHSSSFLLP-SNP---------VDDTTGTRYALIVLNQSLPRFAPLLWEHAKLRLCADGGANRIYDELPLFFPHEDALAIRNRY 78
[Close]
BLAST of HO066208 vs. TrEMBL
Match: B9I9S9_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_572707 PE=4 SV=1)

HSP 1 Score: 90.5077 bits (223), Expect = 5.829e-17
Identity = 44/88 (50.00%), Postives = 59/88 (67.05%), Query Frame = 2 
Query:   26 MDFMCHCSNFLLPPDNPNAAXXXXXXXXXXXXLTYALVVLNQHLPKFAPLLWDHAHIRVCADGGANRVYDEVPLFFPQLNPSHVRTRF 289
            M+ M H S FLLP ++ +++            +TYAL VLN+ LP+F PLLW+HA +RVCADGGANRV+DE+PL FP  +   VR R+
Sbjct:    1 MEVMTHSSTFLLPSNHHSSS------------VTYALAVLNRPLPRFTPLLWNHAQVRVCADGGANRVFDEMPLLFPSDDALDVRQRY 76
[Close]
BLAST of HO066208 vs. TrEMBL
Match: C5XH23_SORBI (Putative uncharacterized protein Sb03g044860 OS=Sorghum bicolor GN=Sb03g044860 PE=4 SV=1)

HSP 1 Score: 88.5817 bits (218), Expect = 2.215e-16
Identity = 47/89 (52.81%), Postives = 55/89 (61.80%), Query Frame = 2 
Query:   35 MCHCSNFLLP----PDNPNAAXXXXXXXXXXXXLTYALVVLNQHLPKFAPLLWDHAHIRVCADGGANRVYDEVPLFFPQLNPSHVRTRF 289
            M H S FLLP    P NP+               TYALVVLNQ LP+FAPLLW  A +RVCADGGANRV+D +P   P  +P+ VRTR+
Sbjct:    6 MTHSSAFLLPSSTAPSNPSGGADTT---------TYALVVLNQRLPRFAPLLWSRARLRVCADGGANRVFDGMPELLPGEDPAEVRTRY 85
[Close]
BLAST of HO066208 vs. TAIR peptide
Match: AT2G44750.2 (| Symbols: TPK2 | thiamin pyrophosphokinase 2 | chr2:18451510-18452754 FORWARD LENGTH=267)

HSP 1 Score: 97.0561 bits (240), Expect = 2.386e-21
Identity = 47/88 (53.41%), Postives = 58/88 (65.91%), Query Frame = 2 
Query:   26 MDFMCHCSNFLLPPDNPNAAXXXXXXXXXXXXLTYALVVLNQHLPKFAPLLWDHAHIRVCADGGANRVYDEVPLFFPQLNPSHVRTRF 289
            MD M H S+FLLP D                   YALVVLNQ+LP+F PLLW+HA +R+CADGGANR+YDE+PLFFP  +P  +R R+
Sbjct:    5 MDVMIHSSSFLLPCDETCGTR-------------YALVVLNQNLPRFTPLLWEHAKLRLCADGGANRIYDELPLFFPHEDPFVIRNRY 79
[Close]
BLAST of HO066208 vs. TAIR peptide
Match: AT2G44750.1 (| Symbols: TPK2 | thiamin pyrophosphokinase 2 | chr2:18451510-18452754 FORWARD LENGTH=265)

HSP 1 Score: 97.0561 bits (240), Expect = 2.386e-21
Identity = 47/88 (53.41%), Postives = 58/88 (65.91%), Query Frame = 2 
Query:   26 MDFMCHCSNFLLPPDNPNAAXXXXXXXXXXXXLTYALVVLNQHLPKFAPLLWDHAHIRVCADGGANRVYDEVPLFFPQLNPSHVRTRF 289
            MD M H S+FLLP D                   YALVVLNQ+LP+F PLLW+HA +R+CADGGANR+YDE+PLFFP  +P  +R R+
Sbjct:    5 MDVMIHSSSFLLPCDETCGTR-------------YALVVLNQNLPRFTPLLWEHAKLRLCADGGANRIYDELPLFFPHEDPFVIRNRY 79
[Close]
BLAST of HO066208 vs. TAIR peptide
Match: AT1G02880.3 (| Symbols: TPK1 | thiamin pyrophosphokinase1 | chr1:643063-644485 REVERSE LENGTH=267)

HSP 1 Score: 95.1301 bits (235), Expect = 9.068e-21
Identity = 46/88 (52.27%), Postives = 57/88 (64.77%), Query Frame = 2 
Query:   26 MDFMCHCSNFLLPPDNPNAAXXXXXXXXXXXXLTYALVVLNQHLPKFAPLLWDHAHIRVCADGGANRVYDEVPLFFPQLNPSHVRTRF 289
            MD M H S+FLLP D  +                YALVVLNQ LP+F PLLW+HA +R+CADGGANR+YDE+PLFFP  +   +R R+
Sbjct:    4 MDVMIHSSSFLLPCDETSTGTR------------YALVVLNQSLPRFTPLLWEHAKLRLCADGGANRIYDELPLFFPNEDALAIRNRY 79
[Close]
BLAST of HO066208 vs. TAIR peptide
Match: AT1G02880.2 (| Symbols: TPK1 | thiamin pyrophosphokinase1 | chr1:643063-644476 REVERSE LENGTH=264)

HSP 1 Score: 95.1301 bits (235), Expect = 9.068e-21
Identity = 46/88 (52.27%), Postives = 57/88 (64.77%), Query Frame = 2 
Query:   26 MDFMCHCSNFLLPPDNPNAAXXXXXXXXXXXXLTYALVVLNQHLPKFAPLLWDHAHIRVCADGGANRVYDEVPLFFPQLNPSHVRTRF 289
            MD M H S+FLLP D  +                YALVVLNQ LP+F PLLW+HA +R+CADGGANR+YDE+PLFFP  +   +R R+
Sbjct:    1 MDVMIHSSSFLLPCDETSTGTR------------YALVVLNQSLPRFTPLLWEHAKLRLCADGGANRIYDELPLFFPNEDALAIRNRY 76
The following BLAST results are available for this feature:
BLAST of HO066208 vs. TrEMBL
Analysis Date: 2010-12-28 (Homology Analysis: Cicer arietinum unigene v1 vs Trembl)
Total hits: 10
Match NameE-valueIdentityDescription
C6TER0_SOYBN6.639e-2968.18Putative uncharacterized protein OS=Glycine max PE... [more]
Q3EBG0_ARATH6.228e-1953.41Putative uncharacterized protein At2g44750.2 OS=Ar... [more]
O80514_ARATH6.228e-1953.41Putative thiamin pyrophosphokinase OS=Arabidopsis ... [more]
B9GS93_POPTR8.134e-1953.41Predicted protein OS=Populus trichocarpa GN=POPTRD... [more]
B9SAN6_RICCO1.062e-1855.68Thiamin pyrophosphokinase, putative OS=Ricinus com... [more]
Q9SRY1_ARATH2.367e-1852.27F22D16.12 protein OS=Arabidopsis thaliana GN=At1g0... [more]
Q3EDJ9_ARATH2.367e-1852.27AT1G02880 protein OS=Arabidopsis thaliana GN=AT1G0... [more]
D7KBD4_ARALY4.037e-1853.41Putative uncharacterized protein OS=Arabidopsis ly... [more]
B9I9S9_POPTR5.829e-1750.00Predicted protein OS=Populus trichocarpa GN=POPTRD... [more]
C5XH23_SORBI2.215e-1652.81Putative uncharacterized protein Sb03g044860 OS=So... [more]
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BLAST of HO066208 vs. TAIR peptide
Analysis Date: 2011-02-03 (Homology Analysis: Cicer arietinum unigene v1 vs TAIR 10 peptide)
Total hits: 4
Match NameE-valueIdentityDescription
AT2G44750.22.386e-2153.41| Symbols: TPK2 | thiamin pyrophosphokinase 2 | ch... [more]
AT2G44750.12.386e-2153.41| Symbols: TPK2 | thiamin pyrophosphokinase 2 | ch... [more]
AT1G02880.39.068e-2152.27| Symbols: TPK1 | thiamin pyrophosphokinase1 | chr... [more]
AT1G02880.29.068e-2152.27| Symbols: TPK1 | thiamin pyrophosphokinase1 | chr... [more]
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InterPro
Analysis Name: InterProScan analysis for Cicer arietinum unigene v1
Date Performed: 2010-12-29
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR007371Thiamin pyrophosphokinase, catalytic domainGENE3D3.40.50.10240no descriptioncoord: 43..79
score: 1.8
IPR007371Thiamin pyrophosphokinase, catalytic domainSUPERFAMILY63999Thiamin pyrophosphokinase, catalytic domaincoord: 42..78
score: 1.1
NoneNo IPR availablePANTHERPTHR13622THIAMIN PYROPHOSPHOKINASEcoord: 52..79
score: 1.5
NoneNo IPR availablePANTHERPTHR13622:SF1THIAMIN PYROPHOSPHOKINASEcoord: 52..79
score: 1.5
NoneNo IPR availableSEGsegsegcoord: 29..40
score

Properties
Property NameValue
Singlet in analysisCicer arietinum unigene v1
Sequences
The following sequences are available for this feature:

EST sequence

>HO066208 ID=HO066208; Name=HO066208; organism=Cicer arietinum; type=EST; length=321bp
TAGCGTGGTCGCGGCCGAGGTACGGATGGATTTCATGTGTCATTGTTCAA
ATTTCCTTCTTCCTCCGGATAATCCAAACGCGGCTTCTTCTTCTTCTTCT
TCTTCTTCTTCTTCTGTCTCACTAACATACGCTCTCGTTGTGCTCAATCA
ACACCTCCCCAAATTCGCTCCTCTGCTTTGGGACCACGCACATATACGAG
TGTGCGCTGATGGAGGTGCCAATCGTGTGTATGATGAAGTGCCTCTTTTC
TTCCCTCAACTTAATCCTTCCCATGTTCGCACCAGGTTTCTGTCAGGTAC
CTGCCCGGGCGGCCGCTCGAA
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Annotated Terms
The following terms have been associated with this EST:
Vocabulary: Molecular Function
TermDefinition
GO:0004788thiamine diphosphokinase activity
GO:0005524ATP binding
Vocabulary: Biological Process
TermDefinition
GO:0009229thiamine diphosphate biosynthetic process
Vocabulary: INTERPRO
TermDefinition
IPR007371TPK_catalytic