Molecular mapping of aluminium resistance loci based on root re-growth and Al-induced fluorescent signals (callose accumulation) in lentil (Lens culinaris Medikus)

Publication Overview
TitleMolecular mapping of aluminium resistance loci based on root re-growth and Al-induced fluorescent signals (callose accumulation) in lentil (Lens culinaris Medikus)
AuthorsSingh CK, Singh D, Tomar RSS, Karwa S, Upadhyaya KC, Pal M
TypeJournal Article
Journal NameMolecular biology reports
Year2018
CitationSingh CK, Singh D, Tomar RSS, Karwa S, Upadhyaya KC, Pal M. Molecular mapping of aluminium resistance loci based on root re-growth and Al-induced fluorescent signals (callose accumulation) in lentil (Lens culinaris Medikus). Molecular biology reports. 2018 Sep 14.

Abstract

Development of aluminium (Al) resistant genotypes through molecular breeding is a major approach for increasing seed yield under acidic conditions. There are no available reports on mapping of Al resistance loci and molecular breeding for Al resistant varieties in lentil. The present study reports a major quantitative trait loci (QTL) for Al resistance using simple sequence repeat (SSR) markers in F2 and F3 mapping populations derived from contrasting parents. Phenotypic response to Al was measured on the bases of root re-growth (RRG), fluorescent signals (callose accumulation) and Al contents in hydroponic assay. After screening 495 SSR markers to search polymorphism between two contrasting parents, 73 polymorphic markers were used for bulk segregation analysis. Two major QTLs were identified using seven trait linked markers, one each for fluorescent signals and RRG mapped on linkage group (LG) 1 under Al stress conditions in F2 mapping population of cross BM-4 × L-4602. One major QTL (qAlt_fs) was localised between PLC_88 and PBA_LC_373, covering 25.9 cM with adjacent marker PLC_88 at a distance of 0.4 cM. Another major QTL (qAlt_rrg) for RRG was in the marker interval of PBA_LC_1247 and PLC_51, covering a distance of 45.7 cM with nearest marker PBA_LC_1247 at a distance of 21.2 cM. Similarly, in F3 families of BM-4 × L-4602 and BM-4 × L-7903, LG-1 was extended to 285.9 and 216.4 cM respectively, having four newly developed genic-SSR markers. These QTLs had a logarithm of odd (LOD) value of 140.5 and 28.8 along with phenotypic variation of 52% and 11% for fluorescent signals and RRG respectively, whereas, qAlt_rrg had LOD of 36 and phenotypic variance of 25% in F3 population of BM-4 × L-4602. Two major QTLs identified in the present study can be further dissected for candidate gene discovery and development of molecular markers for breeding improved varieties with high Al resistance.
Stocks
This publication contains information about 4 stocks:
Stock NameUniquenameType
BM-4BM-4cultivar
BM4/L4602-F2BM4/L4602-F2population
BM4/L4602-F3BM4/L4602-F3population
BM4/L7903-F3BM4/L7903-F3population
Features
This publication contains information about 8 features:
Feature NameUniquenameType
LcgSSR14LcgSSR14genetic_marker
LcgSSR36LcgSSR36genetic_marker
LcgSSR42LcgSSR42genetic_marker
LcgSSR44LcgSSR44genetic_marker
PB01698PB01698genetic_marker
Aluminum toleranceqALT.BM4/L4602.LG01QTL
Aluminum toleranceqALT.BM4/L4602.LG01.F2QTL
Aluminum toleranceqALT.BM4/L4602.LG01.F3QTL
Projects
This publication contains information about 1 projects:
Project NameDescription
Lentil-Aluminum_tolerance-Singh-2018
Featuremaps
This publication contains information about 3 maps:
Map Name
Lentil-BM4/L4602-F2
Lentil-BM4/L4602-F3
Lentil-BM4/L7903-F3
Properties
Additional details for this publication include:
Property NameValue
DOI10.1007/s11033-018-4368-4
eISSN1573-4978
Elocation10.1007/s11033-018-4368-4
ISSN1573-4978
Journal AbbreviationMol. Biol. Rep.
Journal CountryNetherlands
LanguageEnglish
Language Abbreng
Publication Date2018 Sep 14
Publication ModelPrint-Electronic
Publication TypeJournal Article
Published Location
URLhttps://www.ncbi.nlm.nih.gov/pubmed/?term=Molecular+mapping+of+aluminium+resistance+loci+based+on+root+re-growth+and+Al-induced+fluorescent+signals+(callose+accumulation)+in+lentil+(Lens+culinaris+Medikus)
Cross References
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DatabaseAccession
PMID: PubMedPMID:30218353