Vfaba.Hedin2.R1.4g055160.1

BLAST of Vfaba.Hedin2.R1.4g055160.1 vs. UniProtKB/TrEMBL
Match: tr|A0A1S2YVJ2|A0A1S2YVJ2_CICAR (Pectinesterase OS=Cicer arietinum OX=3827 GN=LOC101489363 PE=3 SV=1)

HSP 1 Score: 955.281 bits (2468), Expect = 0.000e+0
Identity = 466/584 (79.79%), Postives = 520/584 (89.04%), Query Frame = 0

Query:    1 MDTIKSFKGYGKVDELEQQAFKKKTRKRITIIIISSLILIAVIVAAVAGILIHKRNTSSNPSPNS-LPNTDLNPATSLKSVCDATQYPHSCIAAISSLPESNTTDPEKLFKLSLKVAMDELSNLTRL--KLKASGDTRLEKAIAVCSSVLEDSLDRLNDSMSMIIADDKILSPAKVRDVETWITAALTDHDTCLDAVGEVNSPAARGVLTQIETAMRNSTEFASNSLAIVSKVIRLLSNFEIPSHHRRLLE-FPEWLGTAERRLLADSVNMTV-DAVVAKDGSGQYTTIGEALQNVKKKSDVRFVVYVKKGIYVENIDLDKNTWNVMIYGDGMTETIVSGSRNFIDGTPTFETATFAVKGKGFIAKDIQFVNTAGASKHQAVAFRSGSDQSVFYKCSFVGYQDTLYAHSNRQFYRECDITGTIDFIFGNAAVVFQNCKIMPRQPLSNQFNTITAQGKKDPNQNSGIVIQKSTIT--PYDNLVAPTYLGRPWKDFSTTLIMKSEIGSFLKPVGWISWVANVEPPSSILYAEYQNTGPGADVTQRVKWVGYKPAIADEEASKFTVESFIQGSEWLPNAAVQFESTL 577
            MDTIKSFKGYGKVDE+EQQAF+KKTRKR+  IIISS+IL+AVI+AAVAG+LIHKR T S+PS +  LPNT+LNPA SLK+VC ATQYP+SC +AISSLP+SNTTDPE+LFKLSLKVA+DEL+ LTR   KL A  DTRL+KAI VC  VL+D+LDRLNDSMS I+   KILSP K+RDVETW++AALTDH TCL+AV EVNS AARGVL +IETAM+NSTEFASNSLAIV+K+I LLSNFE  +HHRRLL  FPEWLG+AER+LLA  VN T  DAVVAKDG+GQ+TTI EAL+ V KKS  RFVVYVKKG Y+ENID+DK TWNVMIYGDGM+ET+V GSRNF+DGTPTFETATFAVKG GFIAKDI+FVNTAGASKHQAVA RSGSD+SVFY+CSFVGYQDTLYAHSNRQFYRECDITGTIDFIFGNAAVVFQNC+IMPRQP+SNQFNTITAQGKKDPNQN+GIVIQ STIT  P DNL APTYLGRPWKDFSTT+IMK+EIGSFL  VGWISWV NVEPPS+I YAEYQNTGPGA V+QRVKW GYKP I+DEEA KFTV+SFIQG EWLP+A+VQFE TL
Sbjct:    1 MDTIKSFKGYGKVDEIEQQAFQKKTRKRMITIIISSVILLAVIIAAVAGVLIHKRTTQSSPSSSDSLPNTELNPAASLKAVCHATQYPNSCFSAISSLPDSNTTDPEQLFKLSLKVAIDELAKLTRFPSKLSARADTRLKKAIGVCGEVLDDALDRLNDSMSSIVGAGKILSPEKIRDVETWLSAALTDHATCLEAVDEVNSTAARGVLREIETAMKNSTEFASNSLAIVTKIIGLLSNFETSNHHRRLLGGFPEWLGSAERKLLATEVNETKPDAVVAKDGTGQFTTITEALKMVNKKSVKRFVVYVKKGEYIENIDIDKKTWNVMIYGDGMSETVVLGSRNFMDGTPTFETATFAVKGMGFIAKDIKFVNTAGASKHQAVALRSGSDRSVFYRCSFVGYQDTLYAHSNRQFYRECDITGTIDFIFGNAAVVFQNCRIMPRQPMSNQFNTITAQGKKDPNQNTGIVIQGSTITTLPNDNLTAPTYLGRPWKDFSTTVIMKTEIGSFLNSVGWISWVFNVEPPSTIFYAEYQNTGPGAGVSQRVKWAGYKPTISDEEALKFTVDSFIQGPEWLPSASVQFEPTL 584          

BLAST of Vfaba.Hedin2.R1.4g055160.1 vs. UniProtKB/TrEMBL
Match: tr|A0A2K3PHK1|A0A2K3PHK1_TRIPR (Pectinesterase OS=Trifolium pratense OX=57577 GN=L195_g011456 PE=3 SV=1)

HSP 1 Score: 940.643 bits (2430), Expect = 0.000e+0
Identity = 486/593 (81.96%), Postives = 537/593 (90.56%), Query Frame = 0

Query:    1 MDTIKSFKGYGKVDELEQQAFKKKTRKRITIIIISSLILIAVIVAAVAGILIHKRNTSSNPSPNSLPNTDLNPATSLKSVCDATQYPHSCIAAISSLPESNTTDPEKLFKLSLKVAMDELSNLTRL---KLKASGDTRLEKAIAVCSSVLEDSLDRLNDSMSMIIADDKILSPAKVRDVETWITAALTDHDTCLDAVGEVNSPAARGVLTQIETAMRNSTEFASNSLAIVSKVIRLLSNFEIPSHHRRLL-EFPEWLGTAERRLLADS-------VNMTVDAVVAKDGSGQYTTIGEALQNVKKKSDVRFVVYVKKGIYVENIDLDKNTWNVMIYGDGMTETIVSGSRNFIDGTPTFETATFAVKGKGFIAKDIQFVNTAGASKHQAVAFRSGSDQSVFYKCSFVGYQDTLYAHSNRQFYRECDITGTIDFIFGNAAVVFQNCKIMPRQPLSNQFNTITAQGKKDPNQNSGIVIQKSTIT--PYDNLVAPTYLGRPWKDFSTTLIMKSEIGSFLKPVGWISWVANVEPPSSILYAEYQNTGPGAD---VTQRVKWVGYKPAIADEEASKFTVESFIQGSEWLPNAAVQFESTL 577
            MDT+KSFKGYGKVDE+EQQAFKKKTRKRITIIIIS++IL+AVI+AAVAGILIHKRNTSS+ S +SLP T L PATSLK+VC+ATQYP+SC +AISSLP+SNTTDPE+LFKLSLKVA+DELSNLTR    K+KASGDTRL+KAI VC SVL DSLDRLNDSMS I++ +KILSP K+RDVETWI+AA+TDHDTCLDAVGE+NS AA+ + ++IETAMRNSTEFASNSLAIV+KVIRLLSNFE+ +HHRRLL +FPEWLG AERRLLA +       +  T DAVVAKDGSGQY TIGEAL+ V KKS  RFVVYVKKG YVENIDLDKNTWNVMIYGDGMTET+VSGSRNF+DGTPTFETATFAVKGKGFIAKDIQF NTAGASKHQAVA RSGSD+SVFY+CSFVG+QDTLYAHSNRQFYR+CDITGTIDFIFGNAAVVFQNCKIMPRQP++NQFNTITAQGKKDPNQN+GIVIQKSTIT  P DNL+APTYLGRPWKDFSTT+IMKS+IGSFLKPVGWISWV NV+PPSSILYAEYQNTGPGA    V QRVKW GYK A+ D EA KFTV+SFIQG EWLPNAAVQF+STL
Sbjct:    1 MDTMKSFKGYGKVDEIEQQAFKKKTRKRITIIIISTVILLAVIIAAVAGILIHKRNTSSSSSSDSLPKTQLTPATSLKAVCEATQYPNSCFSAISSLPDSNTTDPEQLFKLSLKVAIDELSNLTRGFSDKIKASGDTRLKKAIEVCGSVLNDSLDRLNDSMSTIVSGEKILSPTKIRDVETWISAAITDHDTCLDAVGELNSTAAKNIRSEIETAMRNSTEFASNSLAIVTKVIRLLSNFEVSNHHRRLLGDFPEWLGAAERRLLAAAEGNGTTVIPDTPDAVVAKDGSGQYKTIGEALKIVPKKSLKRFVVYVKKGEYVENIDLDKNTWNVMIYGDGMTETVVSGSRNFMDGTPTFETATFAVKGKGFIAKDIQFKNTAGASKHQAVALRSGSDESVFYRCSFVGFQDTLYAHSNRQFYRDCDITGTIDFIFGNAAVVFQNCKIMPRQPMANQFNTITAQGKKDPNQNTGIVIQKSTITTLPNDNLIAPTYLGRPWKDFSTTIIMKSQIGSFLKPVGWISWVFNVDPPSSILYAEYQNTGPGAXGAAVDQRVKWAGYKSALEDGEALKFTVDSFIQGPEWLPNAAVQFDSTL 593          

BLAST of Vfaba.Hedin2.R1.4g055160.1 vs. UniProtKB/TrEMBL
Match: tr|G7L971|G7L971_MEDTR (Pectinesterase OS=Medicago truncatula OX=3880 GN=11445925 PE=3 SV=1)

HSP 1 Score: 935.25 bits (2416), Expect = 0.000e+0
Identity = 489/583 (83.88%), Postives = 538/583 (92.28%), Query Frame = 0

Query:    1 MDTIKSFKGYGKVDELEQQAFKKKTRKRITIIIISSLILIAVIVAAVAGILIHKRNTSSNPSPNSLPNTDLNPATSLKSVCDATQYPHSCIAAISSLPESNTTDPEKLFKLSLKVAMDELS--NLTRLKLKASGDTRLEKAIAVCSSVLEDSLDRLNDSMSMIIADDKILSPAKVRDVETWITAALTDHDTCLDAVGEVNSPAARGVLTQIETAMRNSTEFASNSLAIVSKVIRLLSNFEIPSHHRRLL-EFPEWLGTAERRLLADSVNMTV-DAVVAKDGSGQYTTIGEALQNVKKKSDVRFVVYVKKGIYVENIDLDKNTWNVMIYGDGMTETIVSGSRNFIDGTPTFETATFAVKGKGFIAKDIQFVNTAGASKHQAVAFRSGSDQSVFYKCSFVGYQDTLYAHSNRQFYRECDITGTIDFIFGNAAVVFQNCKIMPRQPLSNQFNTITAQGKKDPNQNSGIVIQKSTIT--PYDNLVAPTYLGRPWKDFSTTLIMKSEIGSFLKPVGWISWVANVEPPSSILYAEYQNTGPGADVTQRVKWVGYKPAIADEEASKFTVESFIQGSEWLPNAAVQFESTL 577
            MDT+KSFKGYGKVDE+EQQAF+KKTRKRITIIIISS+IL+AVI+AAVAGILIHK NT S+ SPNSLPNT+L PATSLK+VC++TQYP+SC ++ISSLP+SNTTDPE+LFKLSLKVA+DELS  +LTR   KA+ + R++KAI VC +VL DSLDRLNDSMS I+   K+LSPAK+RDVETW++AALTDHDTCLDAVGEVNS AARGV+ +IE  MRNSTEFASNSLAIVSKVIRLLSNFE+ +HHRRLL EFPEWLGTAERRLLA  VN TV DAVVAKDGSGQY TIGEAL+ VKKKS  RFVVYVKKG+YVENIDLDKNTWNVMIYGDGMTET+VSGSRN+IDGTPTFETATFAVKGKGFIAKDIQF+NTAGASKHQAVA RSGSDQSVFY+CSFVGYQDTLYAHSNRQFYR+CDITGTIDFIFGNAA VFQNCKIMPRQP+SNQFNTITAQGKKDPNQNSGIVIQKST T  P DNL+APTYLGRPWKDFSTT+IMKSEIGSFLKPVGWISWVANVEPPSSILYAEYQNTGPGADV  RVKW GYKPA+ DE+A KFTV+SFIQG EWLP+A+VQF+ST+
Sbjct:    1 MDTMKSFKGYGKVDEIEQQAFQKKTRKRITIIIISSIILVAVIIAAVAGILIHKHNTESSSSPNSLPNTELTPATSLKAVCESTQYPNSCFSSISSLPDSNTTDPEQLFKLSLKVAIDELSKLSLTRFSEKAT-EPRVKKAIGVCDNVLADSLDRLNDSMSTIVDGGKMLSPAKIRDVETWLSAALTDHDTCLDAVGEVNSTAARGVIPEIERIMRNSTEFASNSLAIVSKVIRLLSNFEVSNHHRRLLGEFPEWLGTAERRLLATVVNETVPDAVVAKDGSGQYKTIGEALKLVKKKSLQRFVVYVKKGVYVENIDLDKNTWNVMIYGDGMTETVVSGSRNYIDGTPTFETATFAVKGKGFIAKDIQFLNTAGASKHQAVAMRSGSDQSVFYRCSFVGYQDTLYAHSNRQFYRDCDITGTIDFIFGNAAAVFQNCKIMPRQPMSNQFNTITAQGKKDPNQNSGIVIQKSTFTTLPGDNLIAPTYLGRPWKDFSTTIIMKSEIGSFLKPVGWISWVANVEPPSSILYAEYQNTGPGADVAGRVKWAGYKPALGDEDAIKFTVDSFIQGPEWLPSASVQFDSTI 582          

BLAST of Vfaba.Hedin2.R1.4g055160.1 vs. UniProtKB/TrEMBL
Match: tr|A0A371FB12|A0A371FB12_MUCPR (Pectinesterase (Fragment) OS=Mucuna pruriens OX=157652 GN=MPE3 PE=3 SV=1)

HSP 1 Score: 912.909 bits (2358), Expect = 0.000e+0
Identity = 437/578 (75.61%), Postives = 500/578 (86.51%), Query Frame = 0

Query:    8 KGYGKVDELEQQAFKKKTRKRITIIIISSLILIAVIVAAVAGILIHKRNTSSNPSPNSLPNTDLNPATSLKSVCDATQYPHSCIAAISSLPESNTTDPEKLFKLSLKVAMDELSNLT----RLKLKASGDTRLEKAIAVCSSVLEDSLDRLNDSMSMIIADD--KILSPAKVRDVETWITAALTDHDTCLDAVGEVNSPAARGVLTQIETAMRNSTEFASNSLAIVSKVIRLLSNFEIPSHHRRLLEFPEWLGTAERRLL-ADSVNMTVDAVVAKDGSGQYTTIGEALQNVKKKSDVRFVVYVKKGIYVENIDLDKNTWNVMIYGDGMTETIVSGSRNFIDGTPTFETATFAVKGKGFIAKDIQFVNTAGASKHQAVAFRSGSDQSVFYKCSFVGYQDTLYAHSNRQFYRECDITGTIDFIFGNAAVVFQNCKIMPRQPLSNQFNTITAQGKKDPNQNSGIVIQKSTITPYD-NLVAPTYLGRPWKDFSTTLIMKSEIGSFLKPVGWISWVANVEPPSSILYAEYQNTGPGADVTQRVKWVGYKPAIADEEASKFTVESFIQGSEWLPNAAVQFESTL 577
            KGYGKVDE+EQQA++KKTRKR+ II +SS++LIAVI+AAVAGI+IHKRN SS PS NS P T+L PA SLK+VCD TQYP+SC AAIS+LPESNTTDPE LFKLSL+VA+DELS L+    +L+  A  D R +KAI VC SV+ D+LDRLNDS+S +      K++SPA V DVETW++A LTD DTCLDA+GE+NS AARG L +I TAMRNSTEFASNSLAIV++++ LLS F    H RRLL FP WLG AERRL+ A S + T DAVVAKDGSGQ+TTIGEAL+ VKKKS+ RF+VYVK+G+YVENIDLDKNTWNVMIYGDG T+T+V GSRNF+DGTPTFETATFAVKGKGFIAKDI FVN AGASKHQAVA RSGSD+SVFY+CSF G+QDTLYAHSNRQFYRECDI+GTIDFIFGNAAVVFQNCKIMPRQPL NQFNTITAQGKKDPNQN+GI+IQKSTITP+D NL APTYLGRPWKDFSTT+IM+S+IGSFLKPVGWISWV NV+P S+I YAEY N+GPGADV+QRVKW GYKP ++D +A KFTV+SFIQG EWLPN AV FESTL
Sbjct:  603 KGYGKVDEVEQQAYQKKTRKRLIIITVSSIVLIAVIIAAVAGIVIHKRNRSSTPSSNSAPQTELTPAASLKAVCDVTQYPNSCFAAISALPESNTTDPELLFKLSLRVAIDELSKLSSFPSKLRANAEHDERFQKAIDVCGSVIGDALDRLNDSVSSLAGASAGKVISPASVTDVETWLSAVLTDQDTCLDALGELNSTAARGALREIGTAMRNSTEFASNSLAIVTRILGLLSKFGSLVHRRRLLGFPTWLGAAERRLMEAKSNDTTPDAVVAKDGSGQFTTIGEALKLVKKKSEERFIVYVKEGLYVENIDLDKNTWNVMIYGDGKTKTVVIGSRNFMDGTPTFETATFAVKGKGFIAKDIGFVNNAGASKHQAVALRSGSDRSVFYRCSFDGFQDTLYAHSNRQFYRECDISGTIDFIFGNAAVVFQNCKIMPRQPLPNQFNTITAQGKKDPNQNTGIIIQKSTITPHDNNLTAPTYLGRPWKDFSTTVIMQSDIGSFLKPVGWISWVPNVDPASTIFYAEYHNSGPGADVSQRVKWAGYKPTLSDSDAVKFTVQSFIQGPEWLPNVAVDFESTL 1180          

HSP 2 Score: 389.037 bits (998), Expect = 9.982e-119
Identity = 221/527 (41.94%), Postives = 308/527 (58.44%), Query Frame = 0

Query:   76 SLKSVCDATQYPHSCIAAISSLPE--SNTTDPEKLFKLSLKVAMD--ELSNLTRLKLKASGD-TRLEK-AIAVCSSVLEDSLDRLNDSMSMIIADDKILSPAK------VRDVETWITAALTDHDTCLDAVGEVNSPAARGVLTQIETAMRNSTEFASNSLAIVSKVI-RLLSNFE--IPSHHRRLL---------EFPEWLGTAERRLLADSVNMTVDAVVAKDGSGQYTTIGEALQNVKKKSDVRFVVYVKKGIYVENIDLDKNTWNVMIYGDGMTETIVSGSRNFIDGTPTFETATFAVKGKGFIAKDIQFVNTAGASKHQAVAFRSGSDQSVFYKCSFVGYQDTLYAHSNRQFYRECDITGTIDFIFGNAAVVFQNCKIMPRQPLSNQFNTITAQGKKDPNQNSGIVIQKSTITPYDNLVA-----PTYLGRPWKDFSTTLIMKSEIGSFLKPVGWISWVANVEPPSSILYAEYQNTGPGADVTQRVKWVGYKPAIADEEASKFTVESFIQGSEWLPNAAVQF 573
            ++KS C +T YP  C +AI++ P      +    + ++SL +     E +  T  KL    D T+ EK A+  C   ++++LD L ++       D  L P K        D++T I+AA+T+  TCLD  G  +  A + V   +E    +     SN+LA+   +    ++N+E  I ++  R L         E+PEW+  A+R+LL  +  +  D VVA DGSG + T+  A+ +   KS+ RF++ +K G+Y EN+++ K   N+M  GDG T TI++ SRN +DG+ TF +AT AV G  F+A+DI F NTAG SKHQAVA R G D S FY C  + YQDTLY H+NRQF+  C I GT+DFIFGN+AVVFQ+C I  R+P S Q N +TAQG+ DPNQN+GIVIQK  I    +L       PTYLGRPWK++S T+IM+S I   + PVGW  W  N    ++++Y EYQNTGPGA  ++RV W G+K      EA  FT  SFI GS WL +    F
Sbjct:   74 TIKSACSSTFYPELCFSAIATEPNVTHKVSSHRDVIEVSLNITARAVEHNYFTVKKLIGRHDLTKREKTALHDCLETIDETLDELKEAQK-----DLQLYPNKKTLYQHADDLKTLISAAITNQVTCLD--GFSHEDADKRVRKALEEGQVHVEHMCSNALAMTKNMTDNDIANYESKIKNNSNRKLVEKINNNNNEWPEWISAADRKLL-QAATVKPDVVVAADGSGDFKTVSAAVDSAPLKSNKRFIIKIKAGVYRENVEVPKKKTNIMFLGDGRTNTIITASRNVVDGSTTFHSATVAVVGGNFLARDITFQNTAGPSKHQAVALRVGGDLSAFYNCDILAYQDTLYVHNNRQFFVNCLIAGTVDFIFGNSAVVFQDCDIHARRPDSGQKNMVTAQGRVDPNQNTGIVIQKCRIGATQDLEPVKGSFPTYLGRPWKEYSRTVIMQSSISDVIDPVGWHEWNGNF-ALTTLVYREYQNTGPGAGTSKRVTWKGFKVITDAAEARAFTPGSFIGGSSWLGSTGFPF 591          

BLAST of Vfaba.Hedin2.R1.4g055160.1 vs. UniProtKB/TrEMBL
Match: tr|A0A4D6MR83|A0A4D6MR83_VIGUN (Pectinesterase OS=Vigna unguiculata OX=3917 GN=DEO72_LG8g459 PE=3 SV=1)

HSP 1 Score: 908.286 bits (2346), Expect = 0.000e+0
Identity = 439/584 (75.17%), Postives = 507/584 (86.82%), Query Frame = 0

Query:    1 MDTIKSFKGYGKVDELEQQAFKKKTRKRITIIIISSLILIAVIVAAVAGILIHKRNTSSNPSPNSLPNTDLNPATSLKSVCDATQYPHSCIAAISSLPESNTTDPEKLFKLSLKVAMDELSNLT----RLKLKASGDTRLEKAIAVCSSVLEDSLDRLNDSMSMI-IADDKILSPAKVRDVETWITAALTDHDTCLDAVGEVNSPAARGVLTQIETAMRNSTEFASNSLAIVSKVIRLLSNFEIPSHHRRLLEFPEWLGTAERRLLADSVN-MTVDAVVAKDGSGQYTTIGEALQNVKKKSDVRFVVYVKKGIYVENIDLDKNTWNVMIYGDGMTETIVSGSRNFIDGTPTFETATFAVKGKGFIAKDIQFVNTAGASKHQAVAFRSGSDQSVFYKCSFVGYQDTLYAHSNRQFYRECDITGTIDFIFGNAAVVFQNCKIMPRQPLSNQFNTITAQGKKDPNQNSGIVIQKSTITPY-DNLVAPTYLGRPWKDFSTTLIMKSEIGSFLKPVGWISWVANVEPPSSILYAEYQNTGPGADVTQRVKWVGYKPAIADEEASKFTVESFIQGSEWLPNAAVQFESTL 577
            MDTIKSFKGYGKVDELEQQA++KKTRKRI II +SS++LIAVI+AAVAG++IH RN  S+PS +S P T L+PA SLK+VCDAT+YP+SC  +ISSLPESNTTDPE LFKLSL+VA+DELS L+    +++  A  D RL+KAI VCSSV+ D+LDRLNDS+S +     +I+S A V DVETW++AALTD DTCLDA+GE+NS AARG L +I+TAMRNSTEFASNSLAIV++++ LLS FE P HHRRLL FPEWLG AERRLL +  N  T D VVAKD SGQ+ TIGEAL++VKKKS+ RF VYVK+G YVENIDLDKNTWNVMIYGDG  +TI+ GSRNF+DGTPTFETATFAVKGKGFIAKDI FVN AGASKHQAVA RSGSD+SVF++CSF G+QDTLYAHSNRQFYR+CDITGTIDFIFGNAAVVFQNCKIMPRQPL NQFNTITAQGKKDPNQN+GI++QKSTITP  +NL APTYLGRPWKDFSTT+IM+SEIGSFL PVGW+SWVANVEP S+I YAEYQNTGPGADV+QRVKW GYKP + D +A KF+V+SFIQG +WLP+AAVQF+S L
Sbjct:  607 MDTIKSFKGYGKVDELEQQAYQKKTRKRIIIITVSSIVLIAVIIAAVAGVVIHNRNNKSSPSSDSAPQTQLSPAASLKAVCDATRYPNSCFTSISSLPESNTTDPELLFKLSLRVAIDELSKLSSFPSKIRANAEHDARLQKAIDVCSSVIGDALDRLNDSISALGTVTGRIVSSASVSDVETWLSAALTDQDTCLDALGELNSTAARGALQEIDTAMRNSTEFASNSLAIVTRILGLLSRFEAPIHHRRLLGFPEWLGAAERRLLEEKNNDSTPDVVVAKDDSGQFKTIGEALKSVKKKSEKRFSVYVKEGSYVENIDLDKNTWNVMIYGDGKDKTIIVGSRNFMDGTPTFETATFAVKGKGFIAKDIGFVNNAGASKHQAVALRSGSDRSVFFRCSFDGFQDTLYAHSNRQFYRDCDITGTIDFIFGNAAVVFQNCKIMPRQPLPNQFNTITAQGKKDPNQNTGIIVQKSTITPLGNNLTAPTYLGRPWKDFSTTVIMQSEIGSFLNPVGWMSWVANVEPASTIFYAEYQNTGPGADVSQRVKWAGYKPTLTDSDADKFSVQSFIQGPDWLPSAAVQFDSNL 1190          

HSP 2 Score: 374.785 bits (961), Expect = 2.672e-113
Identity = 215/530 (40.57%), Postives = 302/530 (56.98%), Query Frame = 0

Query:   77 LKSVCDATQYPHSCIAAISSLPE--SNTTDPEKLFKLSLKVAMDELSN----LTRLKLKASGDTRLEKAIAVCSSVLEDSLDRLNDSM-SMIIADDKILSPAKVRDVETWITAALTDHDTCLDAVGEVNSPAARGVLTQIETAMRNSTEFASNSLAIVSKVIRL-LSNFEIP--------SHHRRLLE------------FPEWLGTAERRLLADSVNMTVDAVVAKDGSGQYTTIGEALQNVKKKSDVRFVVYVKKGIYVENIDLDKNTWNVMIYGDGMTETIVSGSRNFIDGTPTFETATFAVKGKGFIAKDIQFVNTAGASKHQAVAFRSGSDQSVFYKCSFVGYQDTLYAHSNRQFYRECDITGTIDFIFGNAAVVFQNCKIMPRQPLSNQFNTITAQGKKDPNQNSGIVIQKSTITPYDNLVA-----PTYLGRPWKDFSTTLIMKSEIGSFLKPVGWISWVANVEPPSSILYAEYQNTGPGADVTQRVKWVGYKPAIADEEASKFTVESFIQGSEWLPNAAVQF 573
            LKS C +T Y   C +A++S P      T    + +LSL +    + +    + +L  K     R + A+  C   ++++LD L ++   + +  +K        D++T I+AA+T+  TCLD  G  +  A + +   +E    +     SN+LA+   +    ++NFE            +R+LL             +PEW+  A+RRLL  +  +  D VVA DGSG + T+ EA+     KS  RFV+ +K G+Y EN+++ K   N+M  GDG T TI++ SRN +DG+ TF +AT AV G+ F+A+DI F NTAG SKHQAVA R G D S FY C  + YQDTLY H+NRQF+ +C + GT+DFIFGN+AVVFQ+C I  R+P S Q N +TAQG+ DPNQN+GIVIQK  I    +L +      TYLGRPWK++S T+ M+S I   + PVGW  W  N    S+++Y EYQNTGPGA  + RV W G+K      EA  FT  SFI GS WL +    F
Sbjct:   77 LKSACSSTFYSELCFSAVASEPNVTHKITSHRDVIQLSLNITTRAVEHNYFTVKKLTTKHGLTEREKTALHDCLETIDETLDELREAQRDLELYPNKKTLYQHADDLKTLISAAITNQVTCLD--GFSHEDADKRIRKALEKGQVHVEHMCSNALAMTKNMTDTDIANFEQNMKLRDNKVGQNRKLLAEESNAGADGGVVWPEWISAADRRLL-QATAVKADVVVAADGSGDFKTVSEAVDAAPLKSSKRFVIRIKAGVYKENVEVPKKKTNIMFLGDGRTTTIITASRNVVDGSTTFHSATVAVVGENFLARDITFQNTAGPSKHQAVALRVGGDLSAFYNCDILAYQDTLYVHNNRQFFVKCLVAGTVDFIFGNSAVVFQDCDIHARRPDSGQKNMVTAQGRVDPNQNTGIVIQKCRIGATSDLESVKKSFKTYLGRPWKEYSRTVFMQSSISDVIDPVGWHEWSGNF-ALSTLVYREYQNTGPGAGTSNRVSWKGFKVITDAAEARAFTPGSFIGGSSWLDSTGFPF 602          

BLAST of Vfaba.Hedin2.R1.4g055160.1 vs. UniProtKB/TrEMBL
Match: tr|A0A0S3SZY5|A0A0S3SZY5_PHAAN (Pectinesterase OS=Vigna angularis var. angularis OX=157739 GN=Vigan.09G186700 PE=3 SV=1)

HSP 1 Score: 903.279 bits (2333), Expect = 0.000e+0
Identity = 439/584 (75.17%), Postives = 504/584 (86.30%), Query Frame = 0

Query:    1 MDTIKSFKGYGKVDELEQQAFKKKTRKRITIIIISSLILIAVIVAAVAGILIHKRNTSSNPSPNSLPNTDLNPATSLKSVCDATQYPHSCIAAISSLPESNTTDPEKLFKLSLKVAMDELSNLT----RLKLKASGDTRLEKAIAVCSSVLEDSLDRLNDSMSMI-IADDKILSPAKVRDVETWITAALTDHDTCLDAVGEVNSPAARGVLTQIETAMRNSTEFASNSLAIVSKVIRLLSNFEIPSHHRRLLEFPEWLGTAERRLLADSVN-MTVDAVVAKDGSGQYTTIGEALQNVKKKSDVRFVVYVKKGIYVENIDLDKNTWNVMIYGDGMTETIVSGSRNFIDGTPTFETATFAVKGKGFIAKDIQFVNTAGASKHQAVAFRSGSDQSVFYKCSFVGYQDTLYAHSNRQFYRECDITGTIDFIFGNAAVVFQNCKIMPRQPLSNQFNTITAQGKKDPNQNSGIVIQKSTITPY-DNLVAPTYLGRPWKDFSTTLIMKSEIGSFLKPVGWISWVANVEPPSSILYAEYQNTGPGADVTQRVKWVGYKPAIADEEASKFTVESFIQGSEWLPNAAVQFESTL 577
            MDTIKSFKGYGKVDELEQQA++KKTRKRI II +SS++LIAVI+AAVAG++IH RN  S+PS +S   T L+PA SLK+VC AT+YP SC ++ISSLPESNTTDPE LFKLSL+VA+DEL  L+    +L+  A  D RL+KAI VCSSV+ D+LDRLNDS+S +     +I+S A V DVETW++A LTD DTCLDA+GE+NS AARG L +IETAMRNSTEFASNSLAIV+K++RLLS FE P HHRRLLEFP+WLG AERRLL +  N  T DAVVAKDGS Q+ TIGEAL+ VKKKS+ RF VYVK+G YVENIDLDKNTWNVMIYGDG  +T++ GSRNFIDGTPTFETATFAVKGKGFIAKDI FVN AGASKHQAVA RSGSD+SVF++CSF G+QDTLYAHSNRQFYR+CDITGTIDFIFGNAAVVFQNCKIMPRQPL NQFNTITAQGKKDPNQN+GI++QKSTITP  +NL APTYLGRPWKDFSTT+IM+S+IGSFL PVGW+SWV NVEP S+I YAEYQN GPGADV++RVKW GYKP + D +A +FTVESFIQG +WLPNAAVQF+STL
Sbjct:    1 MDTIKSFKGYGKVDELEQQAYQKKTRKRIIIITVSSIVLIAVIIAAVAGVVIHNRNNKSSPSSDSAQQTQLSPAASLKAVCKATRYPDSCFSSISSLPESNTTDPELLFKLSLRVAIDELYKLSSFPSKLRANAEHDARLQKAIDVCSSVIGDALDRLNDSISALGTVTGRIVSSASVNDVETWLSATLTDQDTCLDALGELNSTAARGSLQEIETAMRNSTEFASNSLAIVTKILRLLSQFEKPDHHRRLLEFPQWLGAAERRLLEEKNNESTPDAVVAKDGSEQFKTIGEALKLVKKKSEKRFSVYVKEGSYVENIDLDKNTWNVMIYGDGKDKTVIVGSRNFIDGTPTFETATFAVKGKGFIAKDIGFVNNAGASKHQAVALRSGSDRSVFFRCSFDGFQDTLYAHSNRQFYRDCDITGTIDFIFGNAAVVFQNCKIMPRQPLPNQFNTITAQGKKDPNQNTGIIVQKSTITPLGNNLTAPTYLGRPWKDFSTTVIMQSDIGSFLNPVGWMSWVPNVEPASTIFYAEYQNNGPGADVSKRVKWAGYKPTLTDTDADRFTVESFIQGPDWLPNAAVQFDSTL 584          

BLAST of Vfaba.Hedin2.R1.4g055160.1 vs. UniProtKB/TrEMBL
Match: tr|A0A0L9VPM7|A0A0L9VPM7_PHAAN (Pectinesterase OS=Phaseolus angularis OX=3914 GN=LR48_Vigan11g002700 PE=3 SV=1)

HSP 1 Score: 903.279 bits (2333), Expect = 0.000e+0
Identity = 439/584 (75.17%), Postives = 504/584 (86.30%), Query Frame = 0

Query:    1 MDTIKSFKGYGKVDELEQQAFKKKTRKRITIIIISSLILIAVIVAAVAGILIHKRNTSSNPSPNSLPNTDLNPATSLKSVCDATQYPHSCIAAISSLPESNTTDPEKLFKLSLKVAMDELSNLT----RLKLKASGDTRLEKAIAVCSSVLEDSLDRLNDSMSMI-IADDKILSPAKVRDVETWITAALTDHDTCLDAVGEVNSPAARGVLTQIETAMRNSTEFASNSLAIVSKVIRLLSNFEIPSHHRRLLEFPEWLGTAERRLLADSVN-MTVDAVVAKDGSGQYTTIGEALQNVKKKSDVRFVVYVKKGIYVENIDLDKNTWNVMIYGDGMTETIVSGSRNFIDGTPTFETATFAVKGKGFIAKDIQFVNTAGASKHQAVAFRSGSDQSVFYKCSFVGYQDTLYAHSNRQFYRECDITGTIDFIFGNAAVVFQNCKIMPRQPLSNQFNTITAQGKKDPNQNSGIVIQKSTITPY-DNLVAPTYLGRPWKDFSTTLIMKSEIGSFLKPVGWISWVANVEPPSSILYAEYQNTGPGADVTQRVKWVGYKPAIADEEASKFTVESFIQGSEWLPNAAVQFESTL 577
            MDTIKSFKGYGKVDELEQQA++KKTRKRI II +SS++LIAVI+AAVAG++IH RN  S+PS +S   T L+PA SLK+VC AT+YP SC ++ISSLPESNTTDPE LFKLSL+VA+DEL  L+    +L+  A  D RL+KAI VCSSV+ D+LDRLNDS+S +     +I+S A V DVETW++A LTD DTCLDA+GE+NS AARG L +IETAMRNSTEFASNSLAIV+K++RLLS FE P HHRRLLEFP+WLG AERRLL +  N  T DAVVAKDGS Q+ TIGEAL+ VKKKS+ RF VYVK+G YVENIDLDKNTWNVMIYGDG  +T++ GSRNFIDGTPTFETATFAVKGKGFIAKDI FVN AGASKHQAVA RSGSD+SVF++CSF G+QDTLYAHSNRQFYR+CDITGTIDFIFGNAAVVFQNCKIMPRQPL NQFNTITAQGKKDPNQN+GI++QKSTITP  +NL APTYLGRPWKDFSTT+IM+S+IGSFL PVGW+SWV NVEP S+I YAEYQN GPGADV++RVKW GYKP + D +A +FTVESFIQG +WLPNAAVQF+STL
Sbjct:    1 MDTIKSFKGYGKVDELEQQAYQKKTRKRIIIITVSSIVLIAVIIAAVAGVVIHNRNNKSSPSSDSAQQTQLSPAASLKAVCKATRYPDSCFSSISSLPESNTTDPELLFKLSLRVAIDELYKLSSFPSKLRANAEHDARLQKAIDVCSSVIGDALDRLNDSISALGTVTGRIVSSASVNDVETWLSATLTDQDTCLDALGELNSTAARGSLQEIETAMRNSTEFASNSLAIVTKILRLLSQFEKPDHHRRLLEFPQWLGAAERRLLEEKNNESTPDAVVAKDGSEQFKTIGEALKLVKKKSEKRFSVYVKEGSYVENIDLDKNTWNVMIYGDGKDKTVIVGSRNFIDGTPTFETATFAVKGKGFIAKDIGFVNNAGASKHQAVALRSGSDRSVFFRCSFDGFQDTLYAHSNRQFYRDCDITGTIDFIFGNAAVVFQNCKIMPRQPLPNQFNTITAQGKKDPNQNTGIIVQKSTITPLGNNLTAPTYLGRPWKDFSTTVIMQSDIGSFLNPVGWMSWVPNVEPASTIFYAEYQNNGPGADVSKRVKWAGYKPTLTDTDADRFTVESFIQGPDWLPNAAVQFDSTL 584          

BLAST of Vfaba.Hedin2.R1.4g055160.1 vs. UniProtKB/TrEMBL
Match: tr|K7KZN7|K7KZN7_SOYBN (Pectinesterase OS=Glycine max OX=3847 GN=GLYMA_07G046000 PE=3 SV=1)

HSP 1 Score: 898.656 bits (2321), Expect = 0.000e+0
Identity = 439/587 (74.79%), Postives = 505/587 (86.03%), Query Frame = 0

Query:    1 MDTIKSFKGYGKVDELEQQAFKKKTRKRITIIIISSLILIAVIVAAVAGILIHKRNTSSNPSPNSLPNTDLNPATSLKSVCDATQYPHSCIAAISSLPESNTTDPEKLFKLSLKVAMDELSNLT----RLKLKASGDTRLEKAIAVCSSVLEDSLDRLNDSMSMIIAD----DKILSPAKVRDVETWITAALTDHDTCLDAVGEVNSPAARGVLTQIETAMRNSTEFASNSLAIVSKVIRLLSNFEIPSHHRRLLEFPEWLGTAERRLL-ADSVNMTVDAVVAKDGSGQYTTIGEALQNVKKKSDVRFVVYVKKGIYVENIDLDKNTWNVMIYGDGMTETIVSGSRNFIDGTPTFETATFAVKGKGFIAKDIQFVNTAGASKHQAVAFRSGSDQSVFYKCSFVGYQDTLYAHSNRQFYRECDITGTIDFIFGNAAVVFQNCKIMPRQPLSNQFNTITAQGKKDPNQNSGIVIQKSTITPY-DNLVAPTYLGRPWKDFSTTLIMKSEIGSFLKPVGWISWVANVEPPSSILYAEYQNTGPGADVTQRVKWVGYKPAIADEEASKFTVESFIQGSEWLPNAAVQFESTL 577
            MDTIKSFKGYGKVDELEQQA++KKTRKR+ II +SS++L AVI+AAVAG++IHKRNTSS+PS +S P T+L PA SLK+VC  TQYP+SC +AISSLPESNTTDPE LFKLSL+VA+DELS L+    +L+  A  D RL+KAI VC +V  D+L++LNDS+S + +      KI+SPA V DVETWI+AALTD DTCLDA+ E+NS A+RG L +IETAMRNSTEFASNSLAIV+K++ LLS F+ P HHRRLL FPEWLG AERRLL  +S   T DAVVA DGSGQ+ TIGEAL+ VKKKS+ RFVV+VK+G YVENIDLDKNTWNV I+GDG  +T+V GSRNF+DGTPTFETATFAVKGKGFIAKDI FVN AGASKHQAVA RSGSD+SVF++CSF G+QDTLYAHSNRQFYR+CDITGTIDFIFGNAA VFQNCKIMPRQPL NQFNTITAQGKKDPNQN+GI+IQKS   P  +NL APTYLGRPWKDFSTT+IM+S+IGSFLKPVGWISWV+NVEP S+I YAEYQNTGPGADV+QRVKW GYKP + D EA KFTV+SFIQG EWLPNAAV+F+STL
Sbjct:  599 MDTIKSFKGYGKVDELEQQAYQKKTRKRLIIIAVSSIVLFAVIIAAVAGVVIHKRNTSSSPSSDSPPQTELTPAASLKAVCHVTQYPNSCFSAISSLPESNTTDPELLFKLSLRVAIDELSKLSSFPSKLRANAEHDARLQKAIDVCGNVFGDALEQLNDSISALGSGAAEAGKIISPASVGDVETWISAALTDQDTCLDALAELNSTASRGALREIETAMRNSTEFASNSLAIVTKILGLLSKFDSPIHHRRLLGFPEWLGAAERRLLQVNSSETTPDAVVASDGSGQFRTIGEALRLVKKKSEKRFVVHVKEGRYVENIDLDKNTWNVFIFGDGKEKTVVVGSRNFMDGTPTFETATFAVKGKGFIAKDIGFVNNAGASKHQAVALRSGSDRSVFFRCSFDGFQDTLYAHSNRQFYRDCDITGTIDFIFGNAAAVFQNCKIMPRQPLPNQFNTITAQGKKDPNQNTGIIIQKSKFIPLGNNLTAPTYLGRPWKDFSTTVIMQSDIGSFLKPVGWISWVSNVEPVSTIFYAEYQNTGPGADVSQRVKWAGYKPTLTDVEADKFTVQSFIQGPEWLPNAAVEFDSTL 1185          

HSP 2 Score: 398.667 bits (1023), Expect = 2.376e-122
Identity = 235/580 (40.52%), Postives = 340/580 (58.62%), Query Frame = 0

Query:   23 KKTRKRITIIIISSLILIAVIVAAVAGILIHKRNTSSNPSPNSLPNTDLNPATSLKSVCDATQYPHSCIAAISSLPE--SNTTDPEKLFKLSLKVAMD--ELSNLTRLKLKASGD--TRLEK-AIAVCSSVLEDSLDRLNDSM-SMIIADDKILSPAKVRDVETWITAALTDHDTCLDAVGEVNSPAARGVLTQIETAMRNSTEFASNSLAIVSKV-----------IRLLSNFEIPSHHRRLL-----EFPEWLGTAERRLLADSVNMTVDAVVAKDGSGQYTTIGEALQNVKKKSDVRFVVYVKKGIYVENIDLDKNTWNVMIYGDGMTETIVSGSRNFIDGTPTFETATFAVKGKGFIAKDIQFVNTAGASKHQAVAFRSGSDQSVFYKCSFVGYQDTLYAHSNRQFYRECDITGTIDFIFGNAAVVFQNCKIMPRQPLSNQFNTITAQGKKDPNQNSGIVIQKSTITPYDNLVA-----PTYLGRPWKDFSTTLIMKSEIGSFLKPVGWISWVANVEPPSSILYAEYQNTGPGADVTQRVKWVGYKPAIADEEASKFTVESFIQGSEWLPNAAVQF 573
            KK  K++ + ++++L++ A +VA V G+     + S+  +P SL +   +  T +KS C +T YP  C +AI+S P      T    + +LSLK+     E +  T  KL    D  T+ EK A+  C   ++++LD L ++  ++ +  +K        D++T I+AA+T+  TCLD  G  +  A + V   +E    +     SN+LA+   +           +R+ +N +  + +R+LL     E+PEW+  A+RRLL  S  +  D  VA DGSG + T+ EA+     KS  RFV+ +K G+Y EN+++ K   N+M  GDG T TI++ SRN +DG+ TF +AT AV G  F+A+D+ F NTAG SKHQAVA R G D S F+ C  + +QDTLY H+NRQF+ +C I GT+DFIFGN+AVVFQ+C I  R P S Q N +TAQG+ DPNQN+GIVIQK  I   ++L +      TYLGRPWK++S T+IM+S I   + P+GW  W  N    S+++Y EYQNTGPGA  + RV W GYK      EA ++T  SFI GS WL +    F
Sbjct:   22 KKNHKKLLLSLLATLLVAASLVAIVVGVKNKNSDNSATSTPLSLSH---HSHTIVKSACSSTFYPELCYSAIASEPNVTHKITTNRDVIQLSLKITFRAVEQNYFTVKKLFTEHDDLTKREKTALHDCLETIDETLDELREAQHNLELYPNKKTLYQHADDLKTLISAAITNQVTCLD--GFSHDDADKHVRKALEKGQVHVEHMCSNALAMTKNMTDSDIANYEYNMRVENNGQNGNSNRKLLVENDVEWPEWISAADRRLLQAST-VKADVTVAADGSGDFKTVTEAVDAAPLKSSKRFVIRIKAGVYRENVEVPKKKNNIMFLGDGRTNTIITASRNVVDGSTTFHSATVAVVGSNFLARDLTFQNTAGPSKHQAVALRVGGDLSAFFNCDILAFQDTLYVHNNRQFFVKCLIAGTVDFIFGNSAVVFQDCDIHARLPSSGQKNMVTAQGRVDPNQNTGIVIQKCRIGATNDLESVKKNFKTYLGRPWKEYSRTVIMQSSISDVIDPIGWHEWSGNF-GLSTLVYREYQNTGPGAGTSNRVTWKGYKVITDTAEAREYTPGSFIGGSSWLGSTGFPF 594          

BLAST of Vfaba.Hedin2.R1.4g055160.1 vs. UniProtKB/TrEMBL
Match: tr|V7ANW5|V7ANW5_PHAVU (Pectinesterase OS=Phaseolus vulgaris OX=3885 GN=PHAVU_010G123100g PE=3 SV=1)

HSP 1 Score: 897.886 bits (2319), Expect = 0.000e+0
Identity = 439/581 (75.56%), Postives = 507/581 (87.26%), Query Frame = 0

Query:    1 MDTIKSFKGYGKVDELEQQAFKKKTRKRITIIIISSLILIAVIVAAVAGILIHKRNTSSNPSPNSLPNTDLNPATSLKSVCDATQYPHSCIAAISSLPESNTTDPEKLFKLSLKVAMDELSNL-TRLKLKASGDTRLEKAIAVCSSVLEDSLDRLNDSMSMI-IADDKILSPAKVRDVETWITAALTDHDTCLDAVGEVNSPAARGVLTQIETAMRNSTEFASNSLAIVSKVIRLLSNFEIPSHHRRLLEFPEWLGTAERRLLADSVN-MTVDAVVAKDGSGQYTTIGEALQNVKKKSDVRFVVYVKKGIYVENIDLDKNTWNVMIYGDGMTETIVSGSRNFIDGTPTFETATFAVKGKGFIAKDIQFVNTAGASKHQAVAFRSGSDQSVFYKCSFVGYQDTLYAHSNRQFYRECDITGTIDFIFGNAAVVFQNCKIMPRQPLSNQFNTITAQGKKDPNQNSGIVIQKSTITPY-DNLVAPTYLGRPWKDFSTTLIMKSEIGSFLKPVGWISWVANVEPPSSILYAEYQNTGPGADVTQRVKWVGYKPAIADEEASKFTVESFIQGSEWLPNAAVQFESTL 577
            MDTIKSFKGYGKV+ELEQQA++KKTRKR+ II +SS++LIAVI+AAVAG++IH RN+ S+PS +S+P T+L+PA SLK+VCD T+YP SC ++ISSLPESNTTDPE LFKLSL+VA+DELS+  ++L+  A  D RL+KAI VCSSV  D+LDRLNDS+S +     +I S A V +VETW++AALTD DTCLDAVGE+NS AARG L +IETAMRNSTEFASNSLAIV+K++ LLS FE P HHRRLL FPEWLG AERRLL +  N  T DAVVAKDGSGQ+ TIGEAL+ VKKKS+ RF VYVK+G YVENIDLDKNTWNVMIYGDG  +T V GSRNF+DGTPTFETATFAVKGKGFIAKDI FVN AGASKHQAVA RSGSD+SVF++CSF G+QDTLYAHSNRQFYR+CDITGTIDFIFGNAAVVFQ+CKIMPRQPL NQFNTITAQGKKDPNQN+GI+IQKSTITP+ +NL APTYLGRPWKDFSTT+IM+S+IG+ L PVGW+SWV NVEPP++I YAEYQN+GPGADV+QRVKW GYKP I D  A +FTV+SFIQG EWLPNAAVQF+STL
Sbjct:  612 MDTIKSFKGYGKVNELEQQAYEKKTRKRLIIIAVSSIVLIAVIIAAVAGVVIHNRNSESSPSSDSVPQTELSPAASLKAVCDTTRYPSSCFSSISSLPESNTTDPELLFKLSLRVAIDELSSFPSKLRANAEQDARLQKAIDVCSSVFGDALDRLNDSISALGTVAGRIASSASVSNVETWLSAALTDQDTCLDAVGELNSTAARGALQEIETAMRNSTEFASNSLAIVTKILGLLSRFETPIHHRRLLGFPEWLGAAERRLLEEKNNDSTPDAVVAKDGSGQFKTIGEALKLVKKKSEERFSVYVKEGRYVENIDLDKNTWNVMIYGDGKDKTFVVGSRNFMDGTPTFETATFAVKGKGFIAKDIGFVNNAGASKHQAVALRSGSDRSVFFRCSFDGFQDTLYAHSNRQFYRDCDITGTIDFIFGNAAVVFQSCKIMPRQPLPNQFNTITAQGKKDPNQNTGIIIQKSTITPFGNNLTAPTYLGRPWKDFSTTVIMQSDIGALLNPVGWMSWVPNVEPPTTIFYAEYQNSGPGADVSQRVKWAGYKPTITDRNAEEFTVQSFIQGPEWLPNAAVQFDSTL 1192          

HSP 2 Score: 379.793 bits (974), Expect = 3.409e-115
Identity = 226/534 (42.32%), Postives = 307/534 (57.49%), Query Frame = 0

Query:   77 LKSVCDATQYPHSCIAAISSLPE--SNTTDPEKLFKLSLKVAMD--ELSNLTRLKLKASGD-TRLEK-AIAVCSSVLEDSLDRLNDSMSMIIADDKILSPAK------VRDVETWITAALTDHDTCLDAVGEVNSPAARGVLTQIETAMRNSTEFASNSLAIVSKVIRL-LSNFEIPS--------HHRRLLE-----------FPEWLGTAERRLLADSVNMTVDAVVAKDGSGQYTTIGEALQNVKKKSDVRFVVYVKKGIYVENIDLDKNTWNVMIYGDGMTETIVSGSRNFIDGTPTFETATFAVKGKGFIAKDIQFVNTAGASKHQAVAFRSGSDQSVFYKCSFVGYQDTLYAHSNRQFYRECDITGTIDFIFGNAAVVFQNCKIMPRQPLSNQFNTITAQGKKDPNQNSGIVIQKSTITPYDNLVA-----PTYLGRPWKDFSTTLIMKSEIGSFLKPVGWISWVANVEPPSSILYAEYQNTGPGADVTQRVKWVGYKPAIADEEASKFTVESFIQGSEWLPNAAVQF 573
            +KS C +T Y   C +A++S P      T    + +LSLK+     E +  T  KL   G  T+ EK A+  C   ++++LD L ++       D  L P K        D++T I+AA+T+  TCLD  G  +  A + V   +E    +     SN+LA+   +    ++NFE  +         +R+LL            +PEW+  A+RRLL  +  +  D VVA DGSG + T+ EA++    KS  RFV+ +K G+Y EN+++ K   N+M  GDG T TI++ SRN +DG+ TF +AT AV G  F+A+DI F NTAGASKHQAVA R G D S FY C  + YQDTLY H+NRQF+  C I GT+DFIFGN+AVVFQ+C I  R+P S Q N +TAQG+ DPNQN+GIVIQK  I    +L +      TYLGRPWK++S T+IM+S I   + PVGW  W  N    S+++Y EYQNTGPGA  + RV W G++      EA  FT  SFI GS WL +    F
Sbjct:   83 IKSACSSTFYSELCFSAVASEPNVTHKVTSHRDVIQLSLKITTRAVEHNYFTVKKLSTKGGLTKREKTALHDCLETIDETLDELREAQH-----DLELYPNKKTLYQHADDLKTLISAAITNQVTCLD--GFSHEDADKRVRKALEEGQVHVERMCSNALAMTKNMTDTDIANFEQTTKLRDNNAGQNRKLLAEESNANGDGVVWPEWISAADRRLL-QAAAVNADVVVAADGSGDFKTVSEAVEAAPLKSSKRFVIRIKAGVYRENVEVPKKKTNIMFLGDGRTSTIITASRNVVDGSTTFHSATVAVVGGNFLARDITFQNTAGASKHQAVALRVGGDLSAFYNCDILAYQDTLYVHNNRQFFVNCLIAGTVDFIFGNSAVVFQDCDIHARRPDSGQKNMVTAQGRVDPNQNTGIVIQKCRIGATSDLESVKKSFKTYLGRPWKEYSRTVIMQSSISDVIDPVGWHEWSGNF-ALSTLVYREYQNTGPGAGTSSRVSWKGFQVITDAGEARAFTPGSFIGGSSWLGSTGFPF 607          

BLAST of Vfaba.Hedin2.R1.4g055160.1 vs. UniProtKB/TrEMBL
Match: tr|A0A445JSC4|A0A445JSC4_GLYSO (Pectinesterase OS=Glycine soja OX=3848 GN=D0Y65_016893 PE=3 SV=1)

HSP 1 Score: 897.501 bits (2318), Expect = 0.000e+0
Identity = 440/587 (74.96%), Postives = 504/587 (85.86%), Query Frame = 0

Query:    1 MDTIKSFKGYGKVDELEQQAFKKKTRKRITIIIISSLILIAVIVAAVAGILIHKRNTSSNPSPNSLPNTDLNPATSLKSVCDATQYPHSCIAAISSLPESNTTDPEKLFKLSLKVAMDELSNLT----RLKLKASGDTRLEKAIAVCSSVLEDSLDRLNDSMSMIIAD----DKILSPAKVRDVETWITAALTDHDTCLDAVGEVNSPAARGVLTQIETAMRNSTEFASNSLAIVSKVIRLLSNFEIPSHHRRLLEFPEWLGTAERRLL-ADSVNMTVDAVVAKDGSGQYTTIGEALQNVKKKSDVRFVVYVKKGIYVENIDLDKNTWNVMIYGDGMTETIVSGSRNFIDGTPTFETATFAVKGKGFIAKDIQFVNTAGASKHQAVAFRSGSDQSVFYKCSFVGYQDTLYAHSNRQFYRECDITGTIDFIFGNAAVVFQNCKIMPRQPLSNQFNTITAQGKKDPNQNSGIVIQKSTITPY-DNLVAPTYLGRPWKDFSTTLIMKSEIGSFLKPVGWISWVANVEPPSSILYAEYQNTGPGADVTQRVKWVGYKPAIADEEASKFTVESFIQGSEWLPNAAVQFESTL 577
            MDTIKSFKGYGKVDELEQQA++KKTRKR+ II  SS++L AVI+AAVAG++IHKRNTSS+PS +S P T+L PA SLK+VC  TQYP+SC +AISSLPESNTTDPE LFKLSL+VA+DELS L+    +L+  A  D RL+KAI VC +V  D+L+RLNDS+S + +      KI+SPA V DVETWI+AALTD DTCLDA+ E+NS A+RG L +IETAMRNSTEFASNSLAIV+K++ LLS F+ P HHRRLL FPEWLG AERRLL  +S   T DAVVA DGSGQ+ TIGEAL+ VKKKS+ RFVV+VK+G YVENIDLDKNTWNV I+GDG  +T+V GSRNF+DGTPTFETATFAVKGKGFIAKDI FVN AGASKHQAVA RSGSD+SVF++CSF G+QDTLYAHSNRQFYR+CDITGTIDFIFGNAA VFQNCKIMPRQPL NQFNTITAQGKKDPNQN+GI+IQKS   P  +NL APTYLGRPWKDFSTT+IM+S+IGSFLKPVGWISWV+NVEP S+I YAEYQNTGPGADV+QRVKW GYKP + D EA KFTV+SFIQG EWLPNAAV+F+STL
Sbjct:    1 MDTIKSFKGYGKVDELEQQAYQKKTRKRLIIIAGSSIVLFAVIIAAVAGVVIHKRNTSSSPSSDSPPQTELTPAASLKAVCHVTQYPNSCFSAISSLPESNTTDPELLFKLSLRVAIDELSKLSSFPSKLRANAEHDARLQKAIDVCGNVFGDALERLNDSISALGSGAAEAGKIISPASVGDVETWISAALTDQDTCLDALAELNSTASRGALREIETAMRNSTEFASNSLAIVTKILGLLSKFDSPIHHRRLLGFPEWLGAAERRLLQVNSSETTPDAVVASDGSGQFRTIGEALRLVKKKSEKRFVVHVKEGRYVENIDLDKNTWNVFIFGDGKEKTVVVGSRNFMDGTPTFETATFAVKGKGFIAKDIGFVNNAGASKHQAVALRSGSDRSVFFRCSFDGFQDTLYAHSNRQFYRDCDITGTIDFIFGNAAAVFQNCKIMPRQPLPNQFNTITAQGKKDPNQNTGIIIQKSKFIPLGNNLTAPTYLGRPWKDFSTTVIMQSDIGSFLKPVGWISWVSNVEPVSTIFYAEYQNTGPGADVSQRVKWAGYKPTLTDVEADKFTVQSFIQGPEWLPNAAVEFDSTL 587          

BLAST of Vfaba.Hedin2.R1.4g055160.1 vs. UniProtKB/Swiss-Prot
Match: sp|Q43111|PME3_PHAVU (Pectinesterase 3 OS=Phaseolus vulgaris OX=3885 GN=MPE3 PE=2 SV=1)

HSP 1 Score: 895.575 bits (2313), Expect = 0.000e+0
Identity = 439/581 (75.56%), Postives = 507/581 (87.26%), Query Frame = 0

Query:    1 MDTIKSFKGYGKVDELEQQAFKKKTRKRITIIIISSLILIAVIVAAVAGILIHKRNTSSNPSPNSLPNTDLNPATSLKSVCDATQYPHSCIAAISSLPESNTTDPEKLFKLSLKVAMDELSNL-TRLKLKASGDTRLEKAIAVCSSVLEDSLDRLNDSMSMI-IADDKILSPAKVRDVETWITAALTDHDTCLDAVGEVNSPAARGVLTQIETAMRNSTEFASNSLAIVSKVIRLLSNFEIPSHHRRLLEFPEWLGTAERRLLADSVN-MTVDAVVAKDGSGQYTTIGEALQNVKKKSDVRFVVYVKKGIYVENIDLDKNTWNVMIYGDGMTETIVSGSRNFIDGTPTFETATFAVKGKGFIAKDIQFVNTAGASKHQAVAFRSGSDQSVFYKCSFVGYQDTLYAHSNRQFYRECDITGTIDFIFGNAAVVFQNCKIMPRQPLSNQFNTITAQGKKDPNQNSGIVIQKSTITPY-DNLVAPTYLGRPWKDFSTTLIMKSEIGSFLKPVGWISWVANVEPPSSILYAEYQNTGPGADVTQRVKWVGYKPAIADEEASKFTVESFIQGSEWLPNAAVQFESTL 577
            MDTIKSFKGYGKV+ELEQQA++KKTRKR+ II +SS++LIAVI+AAVAG++IH RN+ S+PS +S+P T+L+PA SLK+VCD T+YP SC ++ISSLPESNTTDPE LFKLSL+VA+DELS+  ++L+  A  D RL+KAI VCSSV  D+LDRLNDS+S +     +I S A V +VETW++AALTD DTCLDAVGE+NS AARG L +IETAMRNSTEFASNSLAIV+K++ LLS FE P HHRRLL FPEWLG AERRLL +  N  T DAVVAKDGSGQ+ TIGEAL+ VKKKS+ RF VYVK+G YVENIDLDKNTWNVMIYGDG  +T V GSRNF+DGTPTFETATFAVKGKGFIAKDI FVN AGASKHQAVA RSGSD+SVF++CSF G+QDTLYAHSNRQFYR+CDITGTIDFIFGNAAVVFQ+CKIMPRQPL NQFNTITAQGKKDPNQN+GI+IQKSTITP+ +NL APTYLGRPWKDFSTT+IM+S+IG+ L PVGW+SWV NVEPP++I YAEYQN+GPGADV+QRVKW GYKP I D  A +FTV+SFIQG EWLPNAAVQF+STL
Sbjct:    1 MDTIKSFKGYGKVNELEQQAYEKKTRKRLIIIAVSSIVLIAVIIAAVAGVVIHNRNSESSPSSDSVPQTELSPAASLKAVCDTTRYPSSCFSSISSLPESNTTDPELLFKLSLRVAIDELSSFPSKLRANAEQDARLQKAIDVCSSVFGDALDRLNDSISALGTVAGRIASSASVSNVETWLSAALTDQDTCLDAVGELNSTAARGALQEIETAMRNSTEFASNSLAIVTKILGLLSRFETPIHHRRLLGFPEWLGAAERRLLEEKNNDSTPDAVVAKDGSGQFKTIGEALKLVKKKSEERFSVYVKEGRYVENIDLDKNTWNVMIYGDGKDKTFVVGSRNFMDGTPTFETATFAVKGKGFIAKDIGFVNNAGASKHQAVALRSGSDRSVFFRCSFDGFQDTLYAHSNRQFYRDCDITGTIDFIFGNAAVVFQSCKIMPRQPLPNQFNTITAQGKKDPNQNTGIIIQKSTITPFGNNLTAPTYLGRPWKDFSTTVIMQSDIGALLNPVGWMSWVPNVEPPTTIFYAEYQNSGPGADVSQRVKWAGYKPTITDRNAEEFTVQSFIQGPEWLPNAAVQFDSTL 581          

BLAST of Vfaba.Hedin2.R1.4g055160.1 vs. UniProtKB/Swiss-Prot
Match: sp|Q43867|PME1_ARATH (Pectinesterase 1 OS=Arabidopsis thaliana OX=3702 GN=PME1 PE=1 SV=1)

HSP 1 Score: 691.419 bits (1783), Expect = 0.000e+0
Identity = 343/589 (58.23%), Postives = 450/589 (76.40%), Query Frame = 0

Query:    1 MDTIKSFKGYGKVDELEQQAFKKKTRKRITIIIISSLILIAVIVAAVAGILIHKRNTSSNPSPNSLPNTDLNPATSLKSVCDATQYPHSCIAAISSLPESNTTDPEKLFKLSLKVAMDELSNLTRLKLKASGDT---RLEKAIAVCSSVLEDSLDRLNDSMSMIIADDK--ILSPAKVRDVETWITAALTDHDTCLDAVGEVN---SPAARGVLTQ-IETAMRNSTEFASNSLAIVSKVIRLLSNFEIPSHHRRLL---------EFPEWLGTAERRLLADSVNMTVDAVVAKDGSGQYTTIGEALQNVKKKSDVRFVVYVKKGIYVENIDLDKNTWNVMIYGDGMTETIVSGSRNFIDGTPTFETATFAVKGKGFIAKDIQFVNTAGASKHQAVAFRSGSDQSVFYKCSFVGYQDTLYAHSNRQFYRECDITGTIDFIFGNAAVVFQNCKIMPRQPLSNQFNTITAQGKKDPNQNSGIVIQKSTITPYDNLVAPTYLGRPWKDFSTTLIMKSEIGSFLKPVGWISWVANVEPPSSILYAEYQNTGPGADVTQRVKWVGYKPAIADEEASKFTVESFIQGSEWLPNAAV 571
            MD++ SFKGYGKVDE +  A KKKTRKR+ ++ IS ++LIAVI+AAV   ++HK    S PSP      +L P+TSLK++C  T++P SCI++IS LP SNTTDPE LFKLSLKV +DEL +++ L  K S +T   R++ A+ VC  ++ED+LDRLND++S I  ++K   LS +K+ D++TW++A +TDH+TC D++ E+    +  A   +TQ +++AM  STEF SNSLAIVSK++  LS+  IP H RR L         +F +W   A RRLL  +  +  D  VA DG+G   T+ EA+  V KKS   FV+YVK G YVEN+ +DK+ WNVMIYGDG  +TI+SGS+NF+DGTPT+ETATFA++GKGFI KDI  +NTAGA+KHQAVAFRSGSD SV+Y+CSF G+QDTLY HSNRQFYR+CD+TGTIDFIFG+AAVVFQ CKIMPRQPLSNQFNTITAQGKKDPNQ+SG+ IQ+ TI+   N++APTYLGRPWK+FSTT+IM++ IG+ ++P GW+SWV+ V+PP+SI+Y EY+NTGPG+DVTQRVKW GYKP ++D EA+KFTV + + G++W+P   V
Sbjct:    1 MDSVNSFKGYGKVDEAQDLALKKKTRKRLLLLSISVVVLIAVIIAAVVATVVHKNKNESTPSPPP----ELTPSTSLKAICSVTRFPESCISSISKLPSSNTTDPETLFKLSLKVIIDELDSISDLPEKLSKETEDERIKSALRVCGDLIEDALDRLNDTVSAIDDEEKKKTLSSSKIEDLKTWLSATVTDHETCFDSLDELKQNKTEYANSTITQNLKSAMSRSTEFTSNSLAIVSKILSALSDLGIPIHRRRRLMSHHHQQSVDFEKW---ARRRLL-QTAGLKPDVTVAGDGTGDVLTVNEAVAKVPKKSLKMFVIYVKSGTYVENVVMDKSKWNVMIYGDGKGKTIISGSKNFVDGTPTYETATFAIQGKGFIMKDIGIINTAGAAKHQAVAFRSGSDFSVYYQCSFDGFQDTLYPHSNRQFYRDCDVTGTIDFIFGSAAVVFQGCKIMPRQPLSNQFNTITAQGKKDPNQSSGMSIQRCTISANGNVIAPTYLGRPWKEFSTTVIMETVIGAVVRPSGWMSWVSGVDPPASIVYGEYKNTGPGSDVTQRVKWAGYKPVMSDAEAAKFTVATLLHGADWIPATGV 581          

BLAST of Vfaba.Hedin2.R1.4g055160.1 vs. UniProtKB/Swiss-Prot
Match: sp|Q9LUL8|PME26_ARATH (Putative pectinesterase/pectinesterase inhibitor 26 OS=Arabidopsis thaliana OX=3702 GN=PME26 PE=2 SV=1)

HSP 1 Score: 548.125 bits (1411), Expect = 0.000e+0
Identity = 274/543 (50.46%), Postives = 377/543 (69.43%), Query Frame = 0

Query:   44 VAAVAGILIHKRNTSSNPSPNSLPNTDLNPATSLKSVCDATQYPHSCIAAISSLPESNTT-DPEKLFKLSLKVAMDELSNLTRLKLKASGDTR---LEKAIAVCSSVLEDSLDRLNDSMS----MIIADDKILSPAKVRDVETWITAALTDHDTCLDAVGE--VNSPAARGVLTQIETAMRNSTEFASNSLAIVSKVIRLLSNFEIPSHHRRLLE---FPEWLGTAERRLLADSVNMTVDAVVAKDGSGQYTTIGEALQNVKKKSDVRFVVYVKKGIYVENIDLDKNTWNVMIYGDGMTETIVSGSRNFIDGTPTFETATFAVKGKGFIAKDIQFVNTAGASKHQAVAFRSGSDQSVFYKCSFVGYQDTLYAHSNRQFYRECDITGTIDFIFGNAAVVFQNCKIMPRQPLSNQFNTITAQGKKDPNQNSGIVIQKSTITPYDNLVAPTYLGRPWKDFSTTLIMKSEIGSFLKPVGWISWVANVE-PPSSILYAEYQNTGPGADVTQRVKWVGYKPAIADEEASKFTVESFIQGSE-WLPNAAV 571
            +A +A   IH+R+   +P     P+    P++ L++VC+ T YP SCI++IS LP S TT DP+ LF+LSL+V  DEL+++  L  K + +T    L+ A++VC+ V + ++D +ND++S    +I    K L+ + + D+ TW+++A+TD  TC D + E   NSP  +    ++++AM NSTEF SNSLAIV++V++  S   IP   RRLL    FP W+    RRLL  + N+T    VA DGSG   T+ EA+  V KK    FV+YVK G YVEN+ + K+ WNV IYGDG  +TI+SGS N +DG  TF T+TFA +GKGF+ KD+  +NTAG  KHQAVAFRS SD+SV+Y+CSF GYQDTLY HSNRQ+YR CD+TGT+DFIFG   VVFQ C I PRQPL NQFNTITA+G ++ NQN+GI I + TI+P  N+ A TYLGRPWK FS T+IM+S IGSF+ P GWI+W +  + PP +I Y EY+N+GPG+D+++RVKW GYKP  +D+EA++FTV+ F++G + W+P A +
Sbjct:  428 LAIIAKFPIHERHGVQSPRLRKSPHP--TPSSVLRTVCNVTNYPASCISSISKLPLSKTTTDPKVLFRLSLQVTFDELNSIVGLPKKLAEETNDEGLKSALSVCADVFDLAVDSVNDTISSLDEVISGGKKNLNSSTIGDLITWLSSAVTDIGTCGDTLDEDNYNSPIPQ----KLKSAMVNSTEFTSNSLAIVAQVLKKPSKSRIPVQGRRLLNSNSFPNWVRPGVRRLL-QAKNLTPHVTVAADGSGDVRTVNEAVWRVPKKGKTMFVIYVKAGTYVENVLMKKDKWNVFIYGDGRDKTIISGSTNMVDGVRTFNTSTFATEGKGFMMKDMGIINTAGPEKHQAVAFRSDSDRSVYYRCSFDGYQDTLYTHSNRQYYRNCDVTGTVDFIFGAGTVVFQGCSIRPRQPLPNQFNTITAEGTQEANQNTGISIHQCTISPNGNVTATTYLGRPWKLFSKTVIMQSVIGSFVNPAGWIAWNSTYDPPPRTIFYREYKNSGPGSDLSKRVKWAGYKPISSDDEAARFTVKYFLRGDDNWIPKAVM 963          

HSP 2 Score: 221.861 bits (564), Expect = 3.209e-61
Identity = 120/254 (47.24%), Postives = 178/254 (70.08%), Query Frame = 0

Query:    1 MDTIKSF-KGYGKVDELEQQAFKKKTRKRITIIIISSLILIAVIVAAVAGILIHKR-NTSSNPSPNSLPNTDLNPATSLKSVCDATQYPHSCIAAISSLPESNTTDPEKLFKLSLKVAMDELSNLTRLKLKASGDTR---LEKAIAVCSSVLEDSLDRLNDSMSM--IIADDKILSPAKVRDVETWITAALTDHDTCLDAVGEVNSPAARGVLTQIETAMRNSTEFASNSLAIVSKVIRLLSNFEIPSHHRRLL 247
            MDT+KS  KGYGKVDE +  A K+KTRKR+  I IS  +L+A+I+++   I IH R   S +P+P+S+P  +L PA SLK+VC  T YP SC ++IS LP SNTTDPE +F+LSL+V +DEL+++  L  K + +T    L+ A++VC  +L+ ++DR+N+++S   ++   KIL+ A + D+ TW++AA+T H TCLDA+ E+ S     +  ++++ M NSTEF SNSLAIV+K++  +S+F IP H RRLL
Sbjct:    1 MDTVKSINKGYGKVDETQDLALKRKTRKRLYQIGISVAVLVAIIISSTVTIAIHSRKGNSPHPTPSSVP--ELTPAASLKTVCSVTNYPVSCFSSISKLPLSNTTDPEVIFRLSLQVVIDELNSIVELPKKLAEETDDEGLKSALSVCEHLLDLAIDRVNETVSAMEVVDGKKILNAATIDDLLTWLSAAVTYHGTCLDALDEI-SHTNSAIPLKLKSGMVNSTEFTSNSLAIVAKILSTISDFGIPIHGRRLL 251          

HSP 3 Score: 160.999 bits (406), Expect = 2.105e-40
Identity = 95/236 (40.25%), Postives = 151/236 (63.98%), Query Frame = 0

Query:   35 SSLILIAVIVAAVA--GILIHKR---NTSSNPSPNSLPNTDLNPATSLKSVCDATQYPHSCIAAISSLPESNTTDPEKLFKLSLKVAMDELSNLTRLKLK---ASGDTRLEKAIAVCSSVLEDSLDRLNDSMSMI--IAD-DKILSPAKVRDVETWITAALTDHDTCLDAVGEV--------NSPAARGVLTQIETAMRNSTEFASNSLAIVSKVIRLLSNFEIPSHHRRLLEFPE 251
            +SL ++A I++ ++  GI IH R   N+S + +P S+P   L PA SL++VC  T+YP SC+++IS LP SNTTDPE LF+LSL+V ++EL+++  L  K    + D RL+ +++VC  V  D++D +ND++S +  + D  KIL  + + +++TW++AA+TDHDTCLDA+ E+        NSP    +  ++++AM NS +F SNSLAI++K          P H R  ++ P 
Sbjct:  226 NSLAIVAKILSTISDFGIPIHGRRLLNSSPHATPISVPK--LTPAASLRNVCSVTRYPASCVSSISKLPSSNTTDPEALFRLSLQVVINELNSIAGLPKKLAEETDDERLKSSLSVCGDVFNDAIDIVNDTISTMEEVGDGKKILKSSTIDEIQTWLSAAVTDHDTCLDALDELSQNKTEYANSP----ISLKLKSAMVNSRKFTSNSLAIIAK---------FPIHERHGVQSPR 446          

BLAST of Vfaba.Hedin2.R1.4g055160.1 vs. UniProtKB/Swiss-Prot
Match: sp|Q9FF78|PME46_ARATH (Probable pectinesterase/pectinesterase inhibitor 46 OS=Arabidopsis thaliana OX=3702 GN=PME46 PE=2 SV=1)

HSP 1 Score: 500.36 bits (1287), Expect = 3.921e-171
Identity = 268/578 (46.37%), Postives = 372/578 (64.36%), Query Frame = 0

Query:   10 YGKVDELEQQAFK--KKTRKRITIIIISSLILIAVIVAAVAGILIHKRNTSSNPSPNSLPNTDLNPATSLKSVCDATQYPHSCIAAISSLPESNTTDPEKLFKLSLKVAMDELSN-LTRLKLKASGDTRLEKAIAVCSSVLEDSLDRLNDSMSMIIADDKILSPAKVRDVETWITAALTDHDTCLDAVGEVNSPAARGVLTQIETAMRNSTEFASNSLAIVSKVIRLLSNFEIPSHHRRLLEFPEW------LGTAERRLLADSVNMTVDA--VVAKDGSGQYTTIGEALQNVKKKSDVRFVVYVKKGIYVENIDLDKNTWNVMIYGDGMTETIVSGSRNFIDGTPTFETATFAVKGKGFIAKDIQFVNTAGASKHQAVAFRSGSDQSVFYKCSFVGYQDTLYAHSNRQFYRECDITGTIDFIFGNAAVVFQNCKIMPRQPLSNQFNTITAQGKKDPNQNSGIVIQKSTITPYDNLV-APTYLGRPWKDFSTTLIMKSEIGSFLKPVGWISWVANVEPPSSILYAEYQNTGPGADVTQRVKWVGYKPAIADEEASKFTVESFIQGSEWLPNAAVQFES 575
            YG++DE EQ   +  +KT+KRI II ISS++L+ ++V AV G     R+ S  P      N     + S+K++CD T +   C   + S P ++ + PE+LFK ++KV + ELS  L         D     A+  C  ++  ++D+LN++M+  + +          D+ TW+++  T  +TC+DA+ E N P+   + T  E  ++NSTE  SN+LAI++ + ++    +     RRLLE          L   E R L +S ++   A  VVAKDGSG+Y TIGEAL  V++K++   ++YVKKG+Y+EN+ ++K  WNV++ GDG ++TIVS   NFIDGTPTFETATFAV GKGF+A+D+ F+NTAG +KHQAVA    +D SVFYKC+   +QDT+YAH+ RQFYR+C I GT+DFIFGNAAVVFQ C+I+PR+P+  Q NTITAQG+KDPNQN+GI I   TI P DNL    T+LGRPWKDFSTT+IMKS +  F+ P GW+ W  +   P +I YAEY N+GPGA    RVKW G K ++  +EA+KFTV+ FI G+ WLP   V F S
Sbjct:    4 YGRLDEHEQAKLEASRKTKKRIAIIAISSIVLVCIVVGAVVGTT--ARDNSKKPPTE---NNGEPISVSVKALCDVTLHKEKCFETLGSAPNASRSSPEELFKYAVKVTITELSKVLDGFSNGEHMDNATSAAMGACVELIGLAVDQLNETMTSSLKN--------FDDLRTWLSSVGTYQETCMDALVEANKPS---LTTFGENHLKNSTEMTSNALAIITWLGKIADTVKF--RRRRLLETGNAKVVVADLPMMEGRRLLESGDLKKKATIVVAKDGSGKYRTIGEALAEVEEKNEKPTIIYVKKGVYLENVRVEKTKWNVVMVGDGQSKTIVSAGLNFIDGTPTFETATFAVFGKGFMARDMGFINTAGPAKHQAVALMVSADLSVFYKCTMDAFQDTMYAHAQRQFYRDCVILGTVDFIFGNAAVVFQKCEILPRRPMKGQQNTITAQGRKDPNQNTGISIHNCTIKPLDNLTDIQTFLGRPWKDFSTTVIMKSFMDKFINPKGWLPWTGDT-APDTIFYAEYLNSGPGASTKNRVKWQGLKTSLTKKEANKFTVKPFIDGNNWLPATKVPFNS 562          

BLAST of Vfaba.Hedin2.R1.4g055160.1 vs. UniProtKB/Swiss-Prot
Match: sp|Q9SG77|PME24_ARATH (Putative pectinesterase/pectinesterase inhibitor 24 OS=Arabidopsis thaliana OX=3702 GN=PME24 PE=3 SV=1)

HSP 1 Score: 489.96 bits (1260), Expect = 4.135e-167
Identity = 251/574 (43.73%), Postives = 365/574 (63.59%), Query Frame = 0

Query:   10 YGKVDELEQQAF--KKKTRKRITIIIISSLILIAVIVAAVAGILIHKRNTSSNPSPNSLPNTDLNPATSLKSVCDATQYPHSCIAAISSLPESNTTDPEKLFKLSLKVAMDELSNLTRLKLKASGDTRLEKAIAVCSSVLEDSLDRLNDSMSMIIADDKILSPAKVRDVETWITAALTDHDTCLDAVGEVNSPAARGVLTQIETAMRNSTEFASNSLAIVSKVIRLLSNFEIPSHHRRLLEFP--EWLGTAERRLLADS-VNMTVDAVVAKDGSGQYTTIGEALQNVKKKSDVRFVVYVKKGIYVENIDLDKNTWNVMIYGDGMTETIVSGSRNFIDGTPTFETATFAVKGKGFIAKDIQFVNTAGASKHQAVAFRSGSDQSVFYKCSFVGYQDTLYAHSNRQFYRECDITGTIDFIFGNAAVVFQNCKIMPRQPLSNQFNTITAQGKKDPNQNSGIVIQKSTITPYDNLV-APTYLGRPWKDFSTTLIMKSEIGSFLKPVGWISWVANVEPPSSILYAEYQNTGPGADVTQRVKWVGYKPAIADEEASKFTVESFIQGSEWLPNAAVQFESTL 577
            YGKVDE E      ++KTRK I II +S +IL  +++ AV G + HK++    P      N   + + S+K+VCD T +   C   + S P +++ +PE+LF+ ++K+ + E+S        + GD +    +  C+ +L+ ++D LN++++     D +  P  V D+ TW+++A T   TC++ +     P         E+ ++NSTE  SN+LAI++ + ++  +F++    RRLL     E    A RRLL  + +    D VVAKDGSG+Y TI  ALQ+V +KS+ R ++YVKKG+Y EN+ ++K  WNV++ GDG +++IVSG  N IDGTPTF+TATFAV GKGF+A+D+ F+NTAG SKHQAVA    +D + FY+C+   YQDTLY H+ RQFYREC I GT+DFIFGN+A V Q+C+I+PR+P+  Q NTITAQG+ DPN N+GI I +  I+P  +L    T+LGRPWK+FSTT+IM S +  F+   GW+ W  +   P +I Y EY+NTGPGA    RVKW G +  ++ +EA++FTV+ FI G  WLP   V F S L
Sbjct:    5 YGKVDEREHVRLEARRKTRKNIAIIAVSLVILAGIVIGAVFGTMAHKKS----PETVETNNNGDSISVSVKAVCDVTLHKEKCFETLGSAPNASSLNPEELFRYAVKITIAEVSKAINAFSSSLGDEKNNITMNACAELLDLTIDNLNNTLTSSSNGD-VTVPELVDDLRTWLSSAGTYQRTCVETLAPDMRPFG-------ESHLKNSTELTSNALAIITWLGKIADSFKL---RRRLLTTADVEVDFHAGRRLLQSTDLRKVADIVVAKDGSGKYRTIKRALQDVPEKSEKRTIIYVKKGVYFENVKVEKKMWNVIVVGDGESKSIVSGRLNVIDGTPTFKTATFAVFGKGFMARDMGFINTAGPSKHQAVALMVSADLTAFYRCTMNAYQDTLYVHAQRQFYRECTIIGTVDFIFGNSASVLQSCRILPRRPMKGQQNTITAQGRTDPNMNTGISIHRCNISPLGDLTDVMTFLGRPWKNFSTTVIMDSYLHGFIDRKGWLPWTGD-SAPDTIFYGEYKNTGPGASTKNRVKWKGLR-FLSTKEANRFTVKPFIDGGRWLPATKVPFRSGL 561          

BLAST of Vfaba.Hedin2.R1.4g055160.1 vs. UniProtKB/Swiss-Prot
Match: sp|Q9FK05|PME61_ARATH (Probable pectinesterase/pectinesterase inhibitor 61 OS=Arabidopsis thaliana OX=3702 GN=PME61 PE=2 SV=1)

HSP 1 Score: 410.223 bits (1053), Expect = 1.404e-135
Identity = 224/579 (38.69%), Postives = 325/579 (56.13%), Query Frame = 0

Query:   21 FKKKTRKRITIIIISSLILIAVIVAAVAGILIHKRNTSSNPSPNSLPNTDLNPATSLKSVCDATQYPHSCIAAISSLPESNTTDPEKLFKLSLKVAMDELSN-------LTRLKLKASGDTRLEKAIAVCSSVLEDSLDRLNDSMS--MIIADDKILSPAKVRDVETWITAALTDHDTCLDAVGEVNSPAARGVLTQIETAMRNSTEFASNSLAIVSKVIRLLSNFEIPSHHRRLL------EFPEWLGTAERRLLADSVN-MTVDAVVAKDGSGQYTTIGEALQNVKKKSDVRFVVYVKKGIYVE-NIDLDKNTWNVMIYGDGMTETIVSGSRNFIDGTPTFETATFAVKGKGFIAKDIQFVNTAGASKHQAVAFRSGSDQSVFYKCSFVGYQDTLYAHSNRQFYRECDITGTIDFIFGNAAVVFQNCKIMPRQPLSNQFNTITAQGKKDPNQNSGIVIQKSTITPYDNLVA-----PTYLGRPWKDFSTTLIMKSEIGSFLKPVGWISWVANVEPPSSILYAEYQNTGPGADVTQRVKWVGYKPAIADEEASKFTVESFIQGSEWLPNAAVQFESTL 577
             +KKT+ ++ +  ++ L++  V     AGI        +  S  + P     P  ++   C  + YP+ CI  +   P S T D  +L  +S    + + S        +T  ++      R+  A   C  +L+DS+D L  ++S  ++++ D+  S     DV TW+++A+T+HDTC D   E+       V  Q+  A+++ +E  SN LAI +  ++ LS   +  ++R+LL      E P WL   +R LL    + +  D  V+KDGSG + TI EA++   + S  RFV+YVK G Y E N+ + +   N+M  GDG  +T+++G ++  D   TF TATFA  G GFI +D+ F N AG +KHQAVA R G D +V Y+C+ +GYQD LY HSNRQF+REC+I GT+DFIFGNAAV+ Q+C I  R+P++ Q  TITAQ +KDPNQN+GI I    +    +L A     PTYLGRPWK +S  + M S++G  + P GW+ W        S+ Y EY N G G+ + QRVKW GY    +  EASKFTV  FI GS WLP+  V F S L
Sbjct:   26 LQKKTKTKLILFTLAVLVVGVVCFGIFAGI-------RAVDSGKTEPKLTRKPTQAISRTCSKSLYPNLCIDTLLDFPGSLTADENELIHISFNATLQKFSKALYTSSTITYTQMPP----RVRSAYDSCLELLDDSVDALTRALSSVVVVSGDESHS-----DVMTWLSSAMTNHDTCTDGFDEIEGQGGE-VKDQVIGAVKDLSEMVSNCLAIFAGKVKDLSGVPV-VNNRKLLGTEETEELPNWLKREDRELLGTPTSAIQADITVSKDGSGTFKTIAEAIKKAPEHSSRRFVIYVKAGRYEEENLKVGRKKTNLMFIGDGKGKTVITGGKSIADDLTTFHTATFAATGAGFIVRDMTFENYAGPAKHQAVALRVGGDHAVVYRCNIIGYQDALYVHSNRQFFRECEIYGTVDFIFGNAAVILQSCNIYARKPMAQQKITITAQNRKDPNQNTGISIHACKLLATPDLEASKGSYPTYLGRPWKLYSRVVYMMSDMGDHIDPRGWLEWNGPFA-LDSLYYGEYMNKGLGSGIGQRVKWPGYHVITSTVEASKFTVAQFISGSSWLPSTGVSFFSGL 585          

BLAST of Vfaba.Hedin2.R1.4g055160.1 vs. UniProtKB/Swiss-Prot
Match: sp|Q8GX86|PME21_ARATH (Probable pectinesterase/pectinesterase inhibitor 21 OS=Arabidopsis thaliana OX=3702 GN=PME21 PE=2 SV=2)

HSP 1 Score: 410.994 bits (1055), Expect = 7.500e-135
Identity = 224/583 (38.42%), Postives = 346/583 (59.35%), Query Frame = 0

Query:   10 YGKVDELEQQAFKKKTRKRITIIIISSLILIAVIVAAVAGILIHKRNTSSNPSPNSLPNTDLNPAT-SLKSVCDATQYPHSCIAAISSLPESNTTDPEKLFKLSLKVAMDELSNLTRLK---LKASGDTRLEKAIAVCSSVLEDSLDRLNDSMSMIIADDKILSPAKVRDVETWITAALTDHDTCLDAVGEVNSPAARGVLTQIETAMRNSTEFASNSLAIVSKVIRLLSNFEIPS-HHRRLLE--FPEWLGTAERRLL---ADSVNMTVDAVVAKDGSGQYTTIGEALQNVKKKSDVRFVVYVKKGIYVENIDLDKNTWNVMIYGDGMTETIVSGSRNFIDGTPTFETATFAVKGKGFIAKDIQFVNTAGASKHQAVAFRSGSDQSVFYKCSFVGYQDTLYAHSNRQFYRECDITGTIDFIFGNAAVVFQNCKIMPRQPLSNQFNTITAQGKKDPNQNSGIVIQKSTITPYDNLVA-----PTYLGRPWKDFSTTLIMKSEIGSFLKPVGWISWVANVEPPSSILYAEYQNTGPGADVTQRVKWVGYKPAIADEEASKFTVESFIQGSEWLPNAAVQFESTL 577
            YG  DE        K ++R  +I ISS++LI+++VA   G+ ++K +  S          ++N +  ++K VC  T Y  +C   +    + NTTDP +L K +  V M ++++  +     ++   D+R   A+  C  +++ +LD L++S   +   +  L    + ++  W++AA++  +TCL+        A       ++ A++ + E   N LAI+S++   +   +IP  + RRLL   FP W+    R+LL   A   ++  D VVA+DGSGQY TI EALQ V KK +  FVV++K G+Y E + ++K   +++  GDG  +TI+SG++N+ DG  T+ TAT A+ G  FIAK+I F NTAGA KHQAVA R  SD+S+F+ C F GYQDTLY HS+RQF+R+C I+GTIDF+FG+AA VFQNC ++ R+PL NQ   ITA G+KDP +++G V Q  TI    + +A       YLGRPWK++S T+IM + I  F++P GW  W+ +     ++ Y+E QNTGPG+ +  RV W G K  +++E+  KFT   +IQG +W+P   V + + L
Sbjct:    3 YGYDDE-------SKRKRRYIVITISSVLLISMVVAVTVGVSLNKHDGDSKG------KAEVNASVKAVKDVCAPTDYRKTCEDTLIKNGK-NTTDPMELVKTAFNVTMKQITDAAKKSQTIMELQKDSRTRMALDQCKELMDYALDELSNSFEELGKFEFHLLDEALINLRIWLSAAISHEETCLEGFQGTQGNAGE----TMKKALKTAIELTHNGLAIISEMSNFVGQMQIPGLNSRRLLAEGFPSWVDQRGRKLLQAAAAYSDVKPDIVVAQDGSGQYKTINEALQFVPKKRNTTFVVHIKAGLYKEYVQVNKTMSHLVFIGDGPDKTIISGNKNYKDGITTYRTATVAIVGNYFIAKNIGFENTAGAIKHQAVAVRVQSDESIFFNCRFDGYQDTLYTHSHRQFFRDCTISGTIDFLFGDAAAVFQNCTLLVRKPLPNQACPITAHGRKDPRESTGFVFQGCTIAGEPDYLAVKETSKAYLGRPWKEYSRTIIMNTFIPDFVQPQGWQPWLGDFG-LKTLFYSEVQNTGPGSALANRVTWAGIK-TLSEEDILKFTPAQYIQGDDWIPGKGVPYTTGL 565          

BLAST of Vfaba.Hedin2.R1.4g055160.1 vs. UniProtKB/Swiss-Prot
Match: sp|Q9M3B0|PME34_ARATH (Probable pectinesterase/pectinesterase inhibitor 34 OS=Arabidopsis thaliana OX=3702 GN=PME34 PE=2 SV=1)

HSP 1 Score: 408.297 bits (1048), Expect = 1.001e-134
Identity = 232/576 (40.28%), Postives = 330/576 (57.29%), Query Frame = 0

Query:   23 KKTRKRI---TIIIISSLILIAVIVAAVAGILIHKRNTSSNPSPNSLPNTDLNPATSLKSVCDATQYPHSCIAAISSLPESNTTDPEK-LFKLSLKVAMDELSNLTRLKLKAS---GDTRLEKAIAVCSSVLEDSLDRLNDSMSMIIADDKILSPAKVRDVETWITAALTDHDTCLDAVGEVNSPAARGVLTQIETAMRNSTEFASNSLAIVSKVIRLLSNFEIPSHHRRLL-------EFPEWLGTAERRLLADSVN-MTVDAVVAKDGSGQYTTIGEALQNVKKKSDVRFVVYVKKGIYVEN-IDLDKNTWNVMIYGDGMTETIVSGSRNFIDGTPTFETATFAVKGKGFIAKDIQFVNTAGASKHQAVAFRSGSDQSVFYKCSFVGYQDTLYAHSNRQFYRECDITGTIDFIFGNAAVVFQNCKIMPRQPLSNQFNTITAQGKKDPNQNSGIVIQKSTITPYDNLVA-----PTYLGRPWKDFSTTLIMKSEIGSFLKPVGWISWVANVEPPSSILYAEYQNTGPGADVTQRVKWVGYKPAIADEEASKFTVESFIQGSEWLPNAAVQFESTL 577
            ++++K++   +I++  SLIL A I A V   L  K N        S+P     P+ ++   C+ T++P  C+ ++   P S      K L  +++ + +   S+        S      R   A   C  +L+DS+D L+ ++S +++     S AK +DV TW++AALT+HDTC +    V+     GV   +  A++N +E  SN LAI S          +P  +RRLL       +FP W+   ER +L   V+ +  D +V+KDG+G   TI EA++   + S  R ++YVK G Y EN + + +   N+M  GDG  +T++SG ++  D   TF TA+FA  G GFIA+DI F N AG +KHQAVA R G+D +V Y+C+ +GYQDTLY HSNRQF+RECDI GT+DFIFGNAAVV QNC I  R+P+  Q NTITAQ +KDPNQN+GI I  S +    +L A      TYLGRPWK FS T+ M S IG  +   GW+ W        ++ Y EY N+GPG+ + QRV W GY+   +  EA++FTV  FI GS WLP+  V F + L
Sbjct:   39 RRSKKKLVVSSIVLAISLILAAAIFAGVRSRL--KLN-------QSVPGLARKPSQAISKACELTRFPELCVDSLMDFPGSLAASSSKDLIHVTVNMTLHHFSHALYSSASLSFVDMPPRARSAYDSCVELLDDSVDALSRALSSVVS-----SSAKPQDVTTWLSAALTNHDTCTEGFDGVDDG---GVKDHMTAALQNLSELVSNCLAIFSASHDGDDFAGVPIQNRRLLGVEEREEKFPRWMRPKEREILEMPVSQIQADIIVSKDGNGTCKTISEAIKKAPQNSTRRIIIYVKAGRYEENNLKVGRKKINLMFVGDGKGKTVISGGKSIFDNITTFHTASFAATGAGFIARDITFENWAGPAKHQAVALRIGADHAVIYRCNIIGYQDTLYVHSNRQFFRECDIYGTVDFIFGNAAVVLQNCSIYARKPMDFQKNTITAQNRKDPNQNTGISIHASRVLAASDLQATNGSTQTYLGRPWKLFSRTVYMMSYIGGHVHTRGWLEWNTTFA-LDTLYYGEYLNSGPGSGLGQRVSWPGYRVINSTAEANRFTVAEFIYGSSWLPSTGVSFLAGL 596          

BLAST of Vfaba.Hedin2.R1.4g055160.1 vs. UniProtKB/Swiss-Prot
Match: sp|Q3E8Z8|PME28_ARATH (Putative pectinesterase/pectinesterase inhibitor 28 OS=Arabidopsis thaliana OX=3702 GN=PME28 PE=2 SV=1)

HSP 1 Score: 412.149 bits (1058), Expect = 1.738e-134
Identity = 225/576 (39.06%), Postives = 341/576 (59.20%), Query Frame = 0

Query:   10 YGKVDELEQQAFKKKTRKRITIIIISSLILIAVIVAAVAGILIHKRNTSSNPSPNSLPNTDLNPATSLKSVCDATQYPHSCIAAISSLPESNTTDPEKLFKLSLKVAMDELSNL---TRLKLKASGDTRLEKAIAVCSSVLEDSLDRLNDSMSMIIADDKILSPAKVRDVETWITAALTDHDTCLDAVGEVNSPAARGVLTQIETAMRNSTEFASNSLAIVSKVIRLLSNFEIPS-HHRRLL--EFPEWLGTAERRLL-ADSVNMTVDAVVAKDGSGQYTTIGEALQNVKKKSDVRFVVYVKKGIYVENIDLDKNTWNVMIYGDGMTETIVSGSRNFIDGTPTFETATFAVKGKGFIAKDIQFVNTAGASKHQAVAFRSGSDQSVFYKCSFVGYQDTLYAHSNRQFYRECDITGTIDFIFGNAAVVFQNCKIMPRQPLSNQFNTITAQGKKDPNQNSGIVIQKSTITPYDNLVA-----PTYLGRPWKDFSTTLIMKSEIGSFLKPVGWISWVANVEPPSSILYAEYQNTGPGADVTQRVKWVGYKPAIADEEASKFTVESFIQGSEWLPNAAVQF 573
            YG  DE        K +KR  II ISS++LI+++VA   G+ ++K + + +    +          ++K VC  T Y  +C   +      +T+DP +L K +    M ++S++   ++  ++   D R + A+  C  +++ ++  L+ S   +   +       +  +  W++A ++   TCLD        A       I+ A++ + +   N LA+V+++   L   +IP  + RRLL  EFP W+    RRLL A    +  D VVA+DGSGQY TI EAL  V KK +  FVV++K+GIY E + ++++  +++  GDG  +T++SGS+++ DG  T++TAT A+ G  FIAK+I F NTAGA KHQAVA R  +D+S+FY C F GYQDTLYAHS+RQFYR+C I+GTIDF+FG+AA VFQNC ++ R+PL NQ   ITA G+KDP +++G V+Q  TI    + +A      TYLGRPWK++S T+IM + I  F+ P GW  W+      +++ Y+E QNTGPGA +T+RV W G K  ++DEE  KFT   +IQG  W+P   V +
Sbjct:    3 YGYDDE------DAKRKKRYVIISISSVLLISMVVAVTIGVSVNKSDNAGDEEITT-------SVKAIKDVCAPTDYKETCEDTLRK-DAKDTSDPLELVKTAFNATMKQISDVAKKSQTMIELQKDPRAKMALDQCKELMDYAIGELSKSFEELGKFEFHKVDEALVKLRIWLSATISHEQTCLDGFQGTQGNAGE----TIKKALKTAVQLTHNGLAMVTEMSNYLGQMQIPEMNSRRLLSQEFPSWMDARARRLLNAPMSEVKPDIVVAQDGSGQYKTINEALNFVPKKKNTTFVVHIKEGIYKEYVQVNRSMTHLVFIGDGPDKTVISGSKSYKDGITTYKTATVAIVGDHFIAKNIAFENTAGAIKHQAVAIRVLADESIFYNCKFDGYQDTLYAHSHRQFYRDCTISGTIDFLFGDAAAVFQNCTLLVRKPLLNQACPITAHGRKDPRESTGFVLQGCTIVGEPDYLAVKEQSKTYLGRPWKEYSRTIIMNTFIPDFVPPEGWQPWLGEFG-LNTLFYSEVQNTGPGAAITKRVTWPGIK-KLSDEEILKFTPAQYIQGDAWIPGKGVPY 558          

BLAST of Vfaba.Hedin2.R1.4g055160.1 vs. UniProtKB/Swiss-Prot
Match: sp|Q43143|PMEU1_SOLLC (Pectinesterase/pectinesterase inhibitor U1 OS=Solanum lycopersicum OX=4081 GN=PMEU1 PE=2 SV=1)

HSP 1 Score: 402.519 bits (1033), Expect = 1.150e-132
Identity = 224/576 (38.89%), Postives = 332/576 (57.64%), Query Frame = 0

Query:   24 KTRKRITIIIISSLILIAVIVAAVAGILIHKRNTSSNPSPNSLPNTDLNPATSLKSVCDATQYPHSCIAAISSLPE--SNTTDPEKLFKLSLKVAMDELSN----LTRLKLKASGDTRLEK-AIAVCSSVLEDSLDRLNDSMSMIIADDKILSPAK------VRDVETWITAALTDHDTCLDAVGEVNSPAARGVLTQIETAMRNSTEFASNSLAIVSKV----IRLLSNFEIPSHHRRLLE----FPEWLGTAERRLLADSVNMTVDAVVAKDGSGQYTTIGEALQNVKKKSDVRFVVYVKKGIYVENIDLDKNTWNVMIYGDGMTETIVSGSRNFIDGTPTFETATFAVKGKGFIAKDIQFVNTAGASKHQAVAFRSGSDQSVFYKCSFVGYQDTLYAHSNRQFYRECDITGTIDFIFGNAAVVFQNCKIMPRQPLSNQFNTITAQGKKDPNQNSGIVIQKSTITPYDNLVA-----PTYLGRPWKDFSTTLIMKSEIGSFLKPVGWISWVANVEPPSSILYAEYQNTGPGADVTQRVKWVGYKPAIADEEASKFTVESFIQGSEWLPNAAVQF 573
            K +K+I + I++S++L+A ++  VAG+  H +N+  +    ++ ++       +KS C  T +P  C +AI ++ +     T  + + +LSL + +  +      +  L     G T  EK A+  C   ++++LD L+ ++     +D  L P K      V D++T I++A+T+ +TCLD  G  +  A + V   +    ++  +  SN+LA++  +    I        P+++R+L+E    +PEWL   +RRLL  S  +T D VVA DGSG Y T+ EA++   +KS  R+V+ +K G+Y EN+D+ K   N+M  GDG + TI++ SRN  DG+ TF +AT        +A+DI F NTAGASKHQAVA   GSD S FY+C  + YQDTLY HSNRQF+ +C + GT+DFIFGN A VFQ+C I  R+P S Q N +TAQG+ DPNQN+GIVIQK  I    +L       PTYLGRPWK++S T+IM+S I   ++P GW  W  N     ++ Y EY NTG GA  + RVKW G+K   +  EA  +T   FI G  WL +    F
Sbjct:   16 KRKKKIYLAIVASVLLVAAVIGVVAGVKSHSKNSDDHADIMAISSS---AHAIVKSACSNTLHPELCYSAIVNVSDFSKKVTSQKDVIELSLNITVKAVRRNYYAVKELIKTRKGLTPREKVALHDCLETMDETLDELHTAV-----EDLELYPNKKSLKEHVEDLKTLISSAITNQETCLD--GFSHDEADKKVRKVLLKGQKHVEKMCSNALAMICNMTDTDIANEMKLSAPANNRKLVEDNGEWPEWLSAGDRRLLQSST-VTPDVVVAADGSGDYKTVSEAVRKAPEKSSKRYVIRIKAGVYRENVDVPKKKTNIMFMGDGKSNTIITASRNVQDGSTTFHSATVVRVAGKVLARDITFQNTAGASKHQAVALCVGSDLSAFYRCDMLAYQDTLYVHSNRQFFVQCLVAGTVDFIFGNGAAVFQDCDIHARRPGSGQKNMVTAQGRTDPNQNTGIVIQKCRIGATSDLRPVQKSFPTYLGRPWKEYSRTVIMQSSITDVIQPAGWHEWNGNF-ALDTLFYGEYANTGAGAPTSGRVKWKGHKVITSSTEAQAYTPGRFIAGGSWLSSTGFPF 579          

BLAST of Vfaba.Hedin2.R1.4g055160.1 vs. Araport11
Match: AT1G53840.1 (| pectin methylesterase 1 | Chr1:20101533-20103458 FORWARD LENGTH=586 | 201606)

HSP 1 Score: 691.419 bits (1783), Expect = 0.000e+0
Identity = 343/589 (58.23%), Postives = 450/589 (76.40%), Query Frame = 0

Query:    1 MDTIKSFKGYGKVDELEQQAFKKKTRKRITIIIISSLILIAVIVAAVAGILIHKRNTSSNPSPNSLPNTDLNPATSLKSVCDATQYPHSCIAAISSLPESNTTDPEKLFKLSLKVAMDELSNLTRLKLKASGDT---RLEKAIAVCSSVLEDSLDRLNDSMSMIIADDK--ILSPAKVRDVETWITAALTDHDTCLDAVGEVN---SPAARGVLTQ-IETAMRNSTEFASNSLAIVSKVIRLLSNFEIPSHHRRLL---------EFPEWLGTAERRLLADSVNMTVDAVVAKDGSGQYTTIGEALQNVKKKSDVRFVVYVKKGIYVENIDLDKNTWNVMIYGDGMTETIVSGSRNFIDGTPTFETATFAVKGKGFIAKDIQFVNTAGASKHQAVAFRSGSDQSVFYKCSFVGYQDTLYAHSNRQFYRECDITGTIDFIFGNAAVVFQNCKIMPRQPLSNQFNTITAQGKKDPNQNSGIVIQKSTITPYDNLVAPTYLGRPWKDFSTTLIMKSEIGSFLKPVGWISWVANVEPPSSILYAEYQNTGPGADVTQRVKWVGYKPAIADEEASKFTVESFIQGSEWLPNAAV 571
            MD++ SFKGYGKVDE +  A KKKTRKR+ ++ IS ++LIAVI+AAV   ++HK    S PSP      +L P+TSLK++C  T++P SCI++IS LP SNTTDPE LFKLSLKV +DEL +++ L  K S +T   R++ A+ VC  ++ED+LDRLND++S I  ++K   LS +K+ D++TW++A +TDH+TC D++ E+    +  A   +TQ +++AM  STEF SNSLAIVSK++  LS+  IP H RR L         +F +W   A RRLL  +  +  D  VA DG+G   T+ EA+  V KKS   FV+YVK G YVEN+ +DK+ WNVMIYGDG  +TI+SGS+NF+DGTPT+ETATFA++GKGFI KDI  +NTAGA+KHQAVAFRSGSD SV+Y+CSF G+QDTLY HSNRQFYR+CD+TGTIDFIFG+AAVVFQ CKIMPRQPLSNQFNTITAQGKKDPNQ+SG+ IQ+ TI+   N++APTYLGRPWK+FSTT+IM++ IG+ ++P GW+SWV+ V+PP+SI+Y EY+NTGPG+DVTQRVKW GYKP ++D EA+KFTV + + G++W+P   V
Sbjct:    1 MDSVNSFKGYGKVDEAQDLALKKKTRKRLLLLSISVVVLIAVIIAAVVATVVHKNKNESTPSPPP----ELTPSTSLKAICSVTRFPESCISSISKLPSSNTTDPETLFKLSLKVIIDELDSISDLPEKLSKETEDERIKSALRVCGDLIEDALDRLNDTVSAIDDEEKKKTLSSSKIEDLKTWLSATVTDHETCFDSLDELKQNKTEYANSTITQNLKSAMSRSTEFTSNSLAIVSKILSALSDLGIPIHRRRRLMSHHHQQSVDFEKW---ARRRLL-QTAGLKPDVTVAGDGTGDVLTVNEAVAKVPKKSLKMFVIYVKSGTYVENVVMDKSKWNVMIYGDGKGKTIISGSKNFVDGTPTYETATFAIQGKGFIMKDIGIINTAGAAKHQAVAFRSGSDFSVYYQCSFDGFQDTLYPHSNRQFYRDCDVTGTIDFIFGSAAVVFQGCKIMPRQPLSNQFNTITAQGKKDPNQSSGMSIQRCTISANGNVIAPTYLGRPWKEFSTTVIMETVIGAVVRPSGWMSWVSGVDPPASIVYGEYKNTGPGSDVTQRVKWAGYKPVMSDAEAAKFTVATLLHGADWIPATGV 581          

BLAST of Vfaba.Hedin2.R1.4g055160.1 vs. Araport11
Match: AT3G14300.1 (| pectinesterase family protein | Chr3:4766905-4769898 REVERSE LENGTH=968 | 201606)

HSP 1 Score: 548.125 bits (1411), Expect = 0.000e+0
Identity = 274/543 (50.46%), Postives = 377/543 (69.43%), Query Frame = 0

Query:   44 VAAVAGILIHKRNTSSNPSPNSLPNTDLNPATSLKSVCDATQYPHSCIAAISSLPESNTT-DPEKLFKLSLKVAMDELSNLTRLKLKASGDTR---LEKAIAVCSSVLEDSLDRLNDSMS----MIIADDKILSPAKVRDVETWITAALTDHDTCLDAVGE--VNSPAARGVLTQIETAMRNSTEFASNSLAIVSKVIRLLSNFEIPSHHRRLLE---FPEWLGTAERRLLADSVNMTVDAVVAKDGSGQYTTIGEALQNVKKKSDVRFVVYVKKGIYVENIDLDKNTWNVMIYGDGMTETIVSGSRNFIDGTPTFETATFAVKGKGFIAKDIQFVNTAGASKHQAVAFRSGSDQSVFYKCSFVGYQDTLYAHSNRQFYRECDITGTIDFIFGNAAVVFQNCKIMPRQPLSNQFNTITAQGKKDPNQNSGIVIQKSTITPYDNLVAPTYLGRPWKDFSTTLIMKSEIGSFLKPVGWISWVANVE-PPSSILYAEYQNTGPGADVTQRVKWVGYKPAIADEEASKFTVESFIQGSE-WLPNAAV 571
            +A +A   IH+R+   +P     P+    P++ L++VC+ T YP SCI++IS LP S TT DP+ LF+LSL+V  DEL+++  L  K + +T    L+ A++VC+ V + ++D +ND++S    +I    K L+ + + D+ TW+++A+TD  TC D + E   NSP  +    ++++AM NSTEF SNSLAIV++V++  S   IP   RRLL    FP W+    RRLL  + N+T    VA DGSG   T+ EA+  V KK    FV+YVK G YVEN+ + K+ WNV IYGDG  +TI+SGS N +DG  TF T+TFA +GKGF+ KD+  +NTAG  KHQAVAFRS SD+SV+Y+CSF GYQDTLY HSNRQ+YR CD+TGT+DFIFG   VVFQ C I PRQPL NQFNTITA+G ++ NQN+GI I + TI+P  N+ A TYLGRPWK FS T+IM+S IGSF+ P GWI+W +  + PP +I Y EY+N+GPG+D+++RVKW GYKP  +D+EA++FTV+ F++G + W+P A +
Sbjct:  428 LAIIAKFPIHERHGVQSPRLRKSPHP--TPSSVLRTVCNVTNYPASCISSISKLPLSKTTTDPKVLFRLSLQVTFDELNSIVGLPKKLAEETNDEGLKSALSVCADVFDLAVDSVNDTISSLDEVISGGKKNLNSSTIGDLITWLSSAVTDIGTCGDTLDEDNYNSPIPQ----KLKSAMVNSTEFTSNSLAIVAQVLKKPSKSRIPVQGRRLLNSNSFPNWVRPGVRRLL-QAKNLTPHVTVAADGSGDVRTVNEAVWRVPKKGKTMFVIYVKAGTYVENVLMKKDKWNVFIYGDGRDKTIISGSTNMVDGVRTFNTSTFATEGKGFMMKDMGIINTAGPEKHQAVAFRSDSDRSVYYRCSFDGYQDTLYTHSNRQYYRNCDVTGTVDFIFGAGTVVFQGCSIRPRQPLPNQFNTITAEGTQEANQNTGISIHQCTISPNGNVTATTYLGRPWKLFSKTVIMQSVIGSFVNPAGWIAWNSTYDPPPRTIFYREYKNSGPGSDLSKRVKWAGYKPISSDDEAARFTVKYFLRGDDNWIPKAVM 963          

HSP 2 Score: 221.861 bits (564), Expect = 3.283e-62
Identity = 120/254 (47.24%), Postives = 178/254 (70.08%), Query Frame = 0

Query:    1 MDTIKSF-KGYGKVDELEQQAFKKKTRKRITIIIISSLILIAVIVAAVAGILIHKR-NTSSNPSPNSLPNTDLNPATSLKSVCDATQYPHSCIAAISSLPESNTTDPEKLFKLSLKVAMDELSNLTRLKLKASGDTR---LEKAIAVCSSVLEDSLDRLNDSMSM--IIADDKILSPAKVRDVETWITAALTDHDTCLDAVGEVNSPAARGVLTQIETAMRNSTEFASNSLAIVSKVIRLLSNFEIPSHHRRLL 247
            MDT+KS  KGYGKVDE +  A K+KTRKR+  I IS  +L+A+I+++   I IH R   S +P+P+S+P  +L PA SLK+VC  T YP SC ++IS LP SNTTDPE +F+LSL+V +DEL+++  L  K + +T    L+ A++VC  +L+ ++DR+N+++S   ++   KIL+ A + D+ TW++AA+T H TCLDA+ E+ S     +  ++++ M NSTEF SNSLAIV+K++  +S+F IP H RRLL
Sbjct:    1 MDTVKSINKGYGKVDETQDLALKRKTRKRLYQIGISVAVLVAIIISSTVTIAIHSRKGNSPHPTPSSVP--ELTPAASLKTVCSVTNYPVSCFSSISKLPLSNTTDPEVIFRLSLQVVIDELNSIVELPKKLAEETDDEGLKSALSVCEHLLDLAIDRVNETVSAMEVVDGKKILNAATIDDLLTWLSAAVTYHGTCLDALDEI-SHTNSAIPLKLKSGMVNSTEFTSNSLAIVAKILSTISDFGIPIHGRRLL 251          

HSP 3 Score: 160.999 bits (406), Expect = 2.153e-41
Identity = 95/236 (40.25%), Postives = 151/236 (63.98%), Query Frame = 0

Query:   35 SSLILIAVIVAAVA--GILIHKR---NTSSNPSPNSLPNTDLNPATSLKSVCDATQYPHSCIAAISSLPESNTTDPEKLFKLSLKVAMDELSNLTRLKLK---ASGDTRLEKAIAVCSSVLEDSLDRLNDSMSMI--IAD-DKILSPAKVRDVETWITAALTDHDTCLDAVGEV--------NSPAARGVLTQIETAMRNSTEFASNSLAIVSKVIRLLSNFEIPSHHRRLLEFPE 251
            +SL ++A I++ ++  GI IH R   N+S + +P S+P   L PA SL++VC  T+YP SC+++IS LP SNTTDPE LF+LSL+V ++EL+++  L  K    + D RL+ +++VC  V  D++D +ND++S +  + D  KIL  + + +++TW++AA+TDHDTCLDA+ E+        NSP    +  ++++AM NS +F SNSLAI++K          P H R  ++ P 
Sbjct:  226 NSLAIVAKILSTISDFGIPIHGRRLLNSSPHATPISVPK--LTPAASLRNVCSVTRYPASCVSSISKLPSSNTTDPEALFRLSLQVVINELNSIAGLPKKLAEETDDERLKSSLSVCGDVFNDAIDIVNDTISTMEEVGDGKKILKSSTIDEIQTWLSAAVTDHDTCLDALDELSQNKTEYANSP----ISLKLKSAMVNSRKFTSNSLAIIAK---------FPIHERHGVQSPR 446          

BLAST of Vfaba.Hedin2.R1.4g055160.1 vs. Araport11
Match: AT5G04960.1 (| Plant invertase/pectin methylesterase inhibitor superfamily | Chr5:1461985-1463809 FORWARD LENGTH=564 | 201606)

HSP 1 Score: 500.36 bits (1287), Expect = 4.012e-172
Identity = 268/578 (46.37%), Postives = 372/578 (64.36%), Query Frame = 0

Query:   10 YGKVDELEQQAFK--KKTRKRITIIIISSLILIAVIVAAVAGILIHKRNTSSNPSPNSLPNTDLNPATSLKSVCDATQYPHSCIAAISSLPESNTTDPEKLFKLSLKVAMDELSN-LTRLKLKASGDTRLEKAIAVCSSVLEDSLDRLNDSMSMIIADDKILSPAKVRDVETWITAALTDHDTCLDAVGEVNSPAARGVLTQIETAMRNSTEFASNSLAIVSKVIRLLSNFEIPSHHRRLLEFPEW------LGTAERRLLADSVNMTVDA--VVAKDGSGQYTTIGEALQNVKKKSDVRFVVYVKKGIYVENIDLDKNTWNVMIYGDGMTETIVSGSRNFIDGTPTFETATFAVKGKGFIAKDIQFVNTAGASKHQAVAFRSGSDQSVFYKCSFVGYQDTLYAHSNRQFYRECDITGTIDFIFGNAAVVFQNCKIMPRQPLSNQFNTITAQGKKDPNQNSGIVIQKSTITPYDNLV-APTYLGRPWKDFSTTLIMKSEIGSFLKPVGWISWVANVEPPSSILYAEYQNTGPGADVTQRVKWVGYKPAIADEEASKFTVESFIQGSEWLPNAAVQFES 575
            YG++DE EQ   +  +KT+KRI II ISS++L+ ++V AV G     R+ S  P      N     + S+K++CD T +   C   + S P ++ + PE+LFK ++KV + ELS  L         D     A+  C  ++  ++D+LN++M+  + +          D+ TW+++  T  +TC+DA+ E N P+   + T  E  ++NSTE  SN+LAI++ + ++    +     RRLLE          L   E R L +S ++   A  VVAKDGSG+Y TIGEAL  V++K++   ++YVKKG+Y+EN+ ++K  WNV++ GDG ++TIVS   NFIDGTPTFETATFAV GKGF+A+D+ F+NTAG +KHQAVA    +D SVFYKC+   +QDT+YAH+ RQFYR+C I GT+DFIFGNAAVVFQ C+I+PR+P+  Q NTITAQG+KDPNQN+GI I   TI P DNL    T+LGRPWKDFSTT+IMKS +  F+ P GW+ W  +   P +I YAEY N+GPGA    RVKW G K ++  +EA+KFTV+ FI G+ WLP   V F S
Sbjct:    4 YGRLDEHEQAKLEASRKTKKRIAIIAISSIVLVCIVVGAVVGTT--ARDNSKKPPTE---NNGEPISVSVKALCDVTLHKEKCFETLGSAPNASRSSPEELFKYAVKVTITELSKVLDGFSNGEHMDNATSAAMGACVELIGLAVDQLNETMTSSLKN--------FDDLRTWLSSVGTYQETCMDALVEANKPS---LTTFGENHLKNSTEMTSNALAIITWLGKIADTVKF--RRRRLLETGNAKVVVADLPMMEGRRLLESGDLKKKATIVVAKDGSGKYRTIGEALAEVEEKNEKPTIIYVKKGVYLENVRVEKTKWNVVMVGDGQSKTIVSAGLNFIDGTPTFETATFAVFGKGFMARDMGFINTAGPAKHQAVALMVSADLSVFYKCTMDAFQDTMYAHAQRQFYRDCVILGTVDFIFGNAAVVFQKCEILPRRPMKGQQNTITAQGRKDPNQNTGISIHNCTIKPLDNLTDIQTFLGRPWKDFSTTVIMKSFMDKFINPKGWLPWTGDT-APDTIFYAEYLNSGPGASTKNRVKWQGLKTSLTKKEANKFTVKPFIDGNNWLPATKVPFNS 562          

BLAST of Vfaba.Hedin2.R1.4g055160.1 vs. Araport11
Match: AT3G10710.1 (| root hair specific 12 | Chr3:3352289-3354237 FORWARD LENGTH=561 | 201606)

HSP 1 Score: 489.96 bits (1260), Expect = 4.231e-168
Identity = 251/574 (43.73%), Postives = 365/574 (63.59%), Query Frame = 0

Query:   10 YGKVDELEQQAF--KKKTRKRITIIIISSLILIAVIVAAVAGILIHKRNTSSNPSPNSLPNTDLNPATSLKSVCDATQYPHSCIAAISSLPESNTTDPEKLFKLSLKVAMDELSNLTRLKLKASGDTRLEKAIAVCSSVLEDSLDRLNDSMSMIIADDKILSPAKVRDVETWITAALTDHDTCLDAVGEVNSPAARGVLTQIETAMRNSTEFASNSLAIVSKVIRLLSNFEIPSHHRRLLEFP--EWLGTAERRLLADS-VNMTVDAVVAKDGSGQYTTIGEALQNVKKKSDVRFVVYVKKGIYVENIDLDKNTWNVMIYGDGMTETIVSGSRNFIDGTPTFETATFAVKGKGFIAKDIQFVNTAGASKHQAVAFRSGSDQSVFYKCSFVGYQDTLYAHSNRQFYRECDITGTIDFIFGNAAVVFQNCKIMPRQPLSNQFNTITAQGKKDPNQNSGIVIQKSTITPYDNLV-APTYLGRPWKDFSTTLIMKSEIGSFLKPVGWISWVANVEPPSSILYAEYQNTGPGADVTQRVKWVGYKPAIADEEASKFTVESFIQGSEWLPNAAVQFESTL 577
            YGKVDE E      ++KTRK I II +S +IL  +++ AV G + HK++    P      N   + + S+K+VCD T +   C   + S P +++ +PE+LF+ ++K+ + E+S        + GD +    +  C+ +L+ ++D LN++++     D +  P  V D+ TW+++A T   TC++ +     P         E+ ++NSTE  SN+LAI++ + ++  +F++    RRLL     E    A RRLL  + +    D VVAKDGSG+Y TI  ALQ+V +KS+ R ++YVKKG+Y EN+ ++K  WNV++ GDG +++IVSG  N IDGTPTF+TATFAV GKGF+A+D+ F+NTAG SKHQAVA    +D + FY+C+   YQDTLY H+ RQFYREC I GT+DFIFGN+A V Q+C+I+PR+P+  Q NTITAQG+ DPN N+GI I +  I+P  +L    T+LGRPWK+FSTT+IM S +  F+   GW+ W  +   P +I Y EY+NTGPGA    RVKW G +  ++ +EA++FTV+ FI G  WLP   V F S L
Sbjct:    5 YGKVDEREHVRLEARRKTRKNIAIIAVSLVILAGIVIGAVFGTMAHKKS----PETVETNNNGDSISVSVKAVCDVTLHKEKCFETLGSAPNASSLNPEELFRYAVKITIAEVSKAINAFSSSLGDEKNNITMNACAELLDLTIDNLNNTLTSSSNGD-VTVPELVDDLRTWLSSAGTYQRTCVETLAPDMRPFG-------ESHLKNSTELTSNALAIITWLGKIADSFKL---RRRLLTTADVEVDFHAGRRLLQSTDLRKVADIVVAKDGSGKYRTIKRALQDVPEKSEKRTIIYVKKGVYFENVKVEKKMWNVIVVGDGESKSIVSGRLNVIDGTPTFKTATFAVFGKGFMARDMGFINTAGPSKHQAVALMVSADLTAFYRCTMNAYQDTLYVHAQRQFYRECTIIGTVDFIFGNSASVLQSCRILPRRPMKGQQNTITAQGRTDPNMNTGISIHRCNISPLGDLTDVMTFLGRPWKNFSTTVIMDSYLHGFIDRKGWLPWTGD-SAPDTIFYGEYKNTGPGASTKNRVKWKGLR-FLSTKEANRFTVKPFIDGGRWLPATKVPFRSGL 561          

BLAST of Vfaba.Hedin2.R1.4g055160.1 vs. Araport11
Match: AT5G53370.1 (| pectin methylesterase PCR fragment F | Chr5:21649683-21651530 REVERSE LENGTH=587 | 201606)

HSP 1 Score: 410.223 bits (1053), Expect = 1.437e-136
Identity = 224/579 (38.69%), Postives = 325/579 (56.13%), Query Frame = 0

Query:   21 FKKKTRKRITIIIISSLILIAVIVAAVAGILIHKRNTSSNPSPNSLPNTDLNPATSLKSVCDATQYPHSCIAAISSLPESNTTDPEKLFKLSLKVAMDELSN-------LTRLKLKASGDTRLEKAIAVCSSVLEDSLDRLNDSMS--MIIADDKILSPAKVRDVETWITAALTDHDTCLDAVGEVNSPAARGVLTQIETAMRNSTEFASNSLAIVSKVIRLLSNFEIPSHHRRLL------EFPEWLGTAERRLLADSVN-MTVDAVVAKDGSGQYTTIGEALQNVKKKSDVRFVVYVKKGIYVE-NIDLDKNTWNVMIYGDGMTETIVSGSRNFIDGTPTFETATFAVKGKGFIAKDIQFVNTAGASKHQAVAFRSGSDQSVFYKCSFVGYQDTLYAHSNRQFYRECDITGTIDFIFGNAAVVFQNCKIMPRQPLSNQFNTITAQGKKDPNQNSGIVIQKSTITPYDNLVA-----PTYLGRPWKDFSTTLIMKSEIGSFLKPVGWISWVANVEPPSSILYAEYQNTGPGADVTQRVKWVGYKPAIADEEASKFTVESFIQGSEWLPNAAVQFESTL 577
             +KKT+ ++ +  ++ L++  V     AGI        +  S  + P     P  ++   C  + YP+ CI  +   P S T D  +L  +S    + + S        +T  ++      R+  A   C  +L+DS+D L  ++S  ++++ D+  S     DV TW+++A+T+HDTC D   E+       V  Q+  A+++ +E  SN LAI +  ++ LS   +  ++R+LL      E P WL   +R LL    + +  D  V+KDGSG + TI EA++   + S  RFV+YVK G Y E N+ + +   N+M  GDG  +T+++G ++  D   TF TATFA  G GFI +D+ F N AG +KHQAVA R G D +V Y+C+ +GYQD LY HSNRQF+REC+I GT+DFIFGNAAV+ Q+C I  R+P++ Q  TITAQ +KDPNQN+GI I    +    +L A     PTYLGRPWK +S  + M S++G  + P GW+ W        S+ Y EY N G G+ + QRVKW GY    +  EASKFTV  FI GS WLP+  V F S L
Sbjct:   26 LQKKTKTKLILFTLAVLVVGVVCFGIFAGI-------RAVDSGKTEPKLTRKPTQAISRTCSKSLYPNLCIDTLLDFPGSLTADENELIHISFNATLQKFSKALYTSSTITYTQMPP----RVRSAYDSCLELLDDSVDALTRALSSVVVVSGDESHS-----DVMTWLSSAMTNHDTCTDGFDEIEGQGGE-VKDQVIGAVKDLSEMVSNCLAIFAGKVKDLSGVPV-VNNRKLLGTEETEELPNWLKREDRELLGTPTSAIQADITVSKDGSGTFKTIAEAIKKAPEHSSRRFVIYVKAGRYEEENLKVGRKKTNLMFIGDGKGKTVITGGKSIADDLTTFHTATFAATGAGFIVRDMTFENYAGPAKHQAVALRVGGDHAVVYRCNIIGYQDALYVHSNRQFFRECEIYGTVDFIFGNAAVILQSCNIYARKPMAQQKITITAQNRKDPNQNTGISIHACKLLATPDLEASKGSYPTYLGRPWKLYSRVVYMMSDMGDHIDPRGWLEWNGPFA-LDSLYYGEYMNKGLGSGIGQRVKWPGYHVITSTVEASKFTVAQFISGSSWLPSTGVSFFSGL 585          

BLAST of Vfaba.Hedin2.R1.4g055160.1 vs. Araport11
Match: AT3G05610.1 (| Plant invertase/pectin methylesterase inhibitor superfamily | Chr3:1625876-1627976 REVERSE LENGTH=669 | 201606)

HSP 1 Score: 410.994 bits (1055), Expect = 7.674e-136
Identity = 224/583 (38.42%), Postives = 346/583 (59.35%), Query Frame = 0

Query:   10 YGKVDELEQQAFKKKTRKRITIIIISSLILIAVIVAAVAGILIHKRNTSSNPSPNSLPNTDLNPAT-SLKSVCDATQYPHSCIAAISSLPESNTTDPEKLFKLSLKVAMDELSNLTRLK---LKASGDTRLEKAIAVCSSVLEDSLDRLNDSMSMIIADDKILSPAKVRDVETWITAALTDHDTCLDAVGEVNSPAARGVLTQIETAMRNSTEFASNSLAIVSKVIRLLSNFEIPS-HHRRLLE--FPEWLGTAERRLL---ADSVNMTVDAVVAKDGSGQYTTIGEALQNVKKKSDVRFVVYVKKGIYVENIDLDKNTWNVMIYGDGMTETIVSGSRNFIDGTPTFETATFAVKGKGFIAKDIQFVNTAGASKHQAVAFRSGSDQSVFYKCSFVGYQDTLYAHSNRQFYRECDITGTIDFIFGNAAVVFQNCKIMPRQPLSNQFNTITAQGKKDPNQNSGIVIQKSTITPYDNLVA-----PTYLGRPWKDFSTTLIMKSEIGSFLKPVGWISWVANVEPPSSILYAEYQNTGPGADVTQRVKWVGYKPAIADEEASKFTVESFIQGSEWLPNAAVQFESTL 577
            YG  DE        K ++R  +I ISS++LI+++VA   G+ ++K +  S          ++N +  ++K VC  T Y  +C   +    + NTTDP +L K +  V M ++++  +     ++   D+R   A+  C  +++ +LD L++S   +   +  L    + ++  W++AA++  +TCL+        A       ++ A++ + E   N LAI+S++   +   +IP  + RRLL   FP W+    R+LL   A   ++  D VVA+DGSGQY TI EALQ V KK +  FVV++K G+Y E + ++K   +++  GDG  +TI+SG++N+ DG  T+ TAT A+ G  FIAK+I F NTAGA KHQAVA R  SD+S+F+ C F GYQDTLY HS+RQF+R+C I+GTIDF+FG+AA VFQNC ++ R+PL NQ   ITA G+KDP +++G V Q  TI    + +A       YLGRPWK++S T+IM + I  F++P GW  W+ +     ++ Y+E QNTGPG+ +  RV W G K  +++E+  KFT   +IQG +W+P   V + + L
Sbjct:    3 YGYDDE-------SKRKRRYIVITISSVLLISMVVAVTVGVSLNKHDGDSKG------KAEVNASVKAVKDVCAPTDYRKTCEDTLIKNGK-NTTDPMELVKTAFNVTMKQITDAAKKSQTIMELQKDSRTRMALDQCKELMDYALDELSNSFEELGKFEFHLLDEALINLRIWLSAAISHEETCLEGFQGTQGNAGE----TMKKALKTAIELTHNGLAIISEMSNFVGQMQIPGLNSRRLLAEGFPSWVDQRGRKLLQAAAAYSDVKPDIVVAQDGSGQYKTINEALQFVPKKRNTTFVVHIKAGLYKEYVQVNKTMSHLVFIGDGPDKTIISGNKNYKDGITTYRTATVAIVGNYFIAKNIGFENTAGAIKHQAVAVRVQSDESIFFNCRFDGYQDTLYTHSHRQFFRDCTISGTIDFLFGDAAAVFQNCTLLVRKPLPNQACPITAHGRKDPRESTGFVFQGCTIAGEPDYLAVKETSKAYLGRPWKEYSRTIIMNTFIPDFVQPQGWQPWLGDFG-LKTLFYSEVQNTGPGSALANRVTWAGIK-TLSEEDILKFTPAQYIQGDDWIPGKGVPYTTGL 565          

BLAST of Vfaba.Hedin2.R1.4g055160.1 vs. Araport11
Match: AT5G53370.2 (| pectin methylesterase PCR fragment F | Chr5:21649683-21651638 REVERSE LENGTH=623 | 201606)

HSP 1 Score: 409.068 bits (1050), Expect = 1.009e-135
Identity = 225/579 (38.86%), Postives = 325/579 (56.13%), Query Frame = 0

Query:   21 FKKKTRKRITIIIISSLILIAVIVAAVAGILIHKRNTSSNPSPNSLPNTDLNPATSLKSVCDATQYPHSCIAAISSLPESNTTDPEKLFKLSLKVAMDELSN-------LTRLKLKASGDTRLEKAIAVCSSVLEDSLDRLNDSMS--MIIADDKILSPAKVRDVETWITAALTDHDTCLDAVGEVNSPAARGVLTQIETAMRNSTEFASNSLAIVSKVIRLLSNFEIPSHHRRLL------EFPEWLGTAERRLLADSVN-MTVDAVVAKDGSGQYTTIGEALQNVKKKSDVRFVVYVKKGIYVE-NIDLDKNTWNVMIYGDGMTETIVSGSRNFIDGTPTFETATFAVKGKGFIAKDIQFVNTAGASKHQAVAFRSGSDQSVFYKCSFVGYQDTLYAHSNRQFYRECDITGTIDFIFGNAAVVFQNCKIMPRQPLSNQFNTITAQGKKDPNQNSGIVIQKSTITPYDNLVA-----PTYLGRPWKDFSTTLIMKSEIGSFLKPVGWISWVANVEPPSSILYAEYQNTGPGADVTQRVKWVGYKPAIADEEASKFTVESFIQGSEWLPNAAVQFESTL 577
             +KKT+ ++ +  ++ L++  V     AGI    R   S  +    P     P  ++   C  + YP+ CI  +   P S T D  +L  +S    + + S        +T  ++      R+  A   C  +L+DS+D L  ++S  ++++ D+  S     DV TW+++A+T+HDTC D   E+       V  Q+  A+++ +E  SN LAI +  ++ LS   +  ++R+LL      E P WL   +R LL    + +  D  V+KDGSG + TI EA++   + S  RFV+YVK G Y E N+ + +   N+M  GDG  +T+++G ++  D   TF TATFA  G GFI +D+ F N AG +KHQAVA R G D +V Y+C+ +GYQD LY HSNRQF+REC+I GT+DFIFGNAAV+ Q+C I  R+P++ Q  TITAQ +KDPNQN+GI I    +    +L A     PTYLGRPWK +S  + M S++G  + P GW+ W        S+ Y EY N G G+ + QRVKW GY    +  EASKFTV  FI GS WLP+  V F S L
Sbjct:   62 LQKKTKTKLILFTLAVLVVGVVCFGIFAGI----RAVDSGKTE---PKLTRKPTQAISRTCSKSLYPNLCIDTLLDFPGSLTADENELIHISFNATLQKFSKALYTSSTITYTQMPP----RVRSAYDSCLELLDDSVDALTRALSSVVVVSGDESHS-----DVMTWLSSAMTNHDTCTDGFDEIEGQGGE-VKDQVIGAVKDLSEMVSNCLAIFAGKVKDLSGVPV-VNNRKLLGTEETEELPNWLKREDRELLGTPTSAIQADITVSKDGSGTFKTIAEAIKKAPEHSSRRFVIYVKAGRYEEENLKVGRKKTNLMFIGDGKGKTVITGGKSIADDLTTFHTATFAATGAGFIVRDMTFENYAGPAKHQAVALRVGGDHAVVYRCNIIGYQDALYVHSNRQFFRECEIYGTVDFIFGNAAVILQSCNIYARKPMAQQKITITAQNRKDPNQNTGISIHACKLLATPDLEASKGSYPTYLGRPWKLYSRVVYMMSDMGDHIDPRGWLEWNGPFA-LDSLYYGEYMNKGLGSGIGQRVKWPGYHVITSTVEASKFTVAQFISGSSWLPSTGVSFFSGL 621          

BLAST of Vfaba.Hedin2.R1.4g055160.1 vs. Araport11
Match: AT3G49220.1 (| Plant invertase/pectin methylesterase inhibitor superfamily | Chr3:18249840-18253647 FORWARD LENGTH=598 | 201606)

HSP 1 Score: 408.297 bits (1048), Expect = 1.024e-135
Identity = 232/576 (40.28%), Postives = 330/576 (57.29%), Query Frame = 0

Query:   23 KKTRKRI---TIIIISSLILIAVIVAAVAGILIHKRNTSSNPSPNSLPNTDLNPATSLKSVCDATQYPHSCIAAISSLPESNTTDPEK-LFKLSLKVAMDELSNLTRLKLKAS---GDTRLEKAIAVCSSVLEDSLDRLNDSMSMIIADDKILSPAKVRDVETWITAALTDHDTCLDAVGEVNSPAARGVLTQIETAMRNSTEFASNSLAIVSKVIRLLSNFEIPSHHRRLL-------EFPEWLGTAERRLLADSVN-MTVDAVVAKDGSGQYTTIGEALQNVKKKSDVRFVVYVKKGIYVEN-IDLDKNTWNVMIYGDGMTETIVSGSRNFIDGTPTFETATFAVKGKGFIAKDIQFVNTAGASKHQAVAFRSGSDQSVFYKCSFVGYQDTLYAHSNRQFYRECDITGTIDFIFGNAAVVFQNCKIMPRQPLSNQFNTITAQGKKDPNQNSGIVIQKSTITPYDNLVA-----PTYLGRPWKDFSTTLIMKSEIGSFLKPVGWISWVANVEPPSSILYAEYQNTGPGADVTQRVKWVGYKPAIADEEASKFTVESFIQGSEWLPNAAVQFESTL 577
            ++++K++   +I++  SLIL A I A V   L  K N        S+P     P+ ++   C+ T++P  C+ ++   P S      K L  +++ + +   S+        S      R   A   C  +L+DS+D L+ ++S +++     S AK +DV TW++AALT+HDTC +    V+     GV   +  A++N +E  SN LAI S          +P  +RRLL       +FP W+   ER +L   V+ +  D +V+KDG+G   TI EA++   + S  R ++YVK G Y EN + + +   N+M  GDG  +T++SG ++  D   TF TA+FA  G GFIA+DI F N AG +KHQAVA R G+D +V Y+C+ +GYQDTLY HSNRQF+RECDI GT+DFIFGNAAVV QNC I  R+P+  Q NTITAQ +KDPNQN+GI I  S +    +L A      TYLGRPWK FS T+ M S IG  +   GW+ W        ++ Y EY N+GPG+ + QRV W GY+   +  EA++FTV  FI GS WLP+  V F + L
Sbjct:   39 RRSKKKLVVSSIVLAISLILAAAIFAGVRSRL--KLN-------QSVPGLARKPSQAISKACELTRFPELCVDSLMDFPGSLAASSSKDLIHVTVNMTLHHFSHALYSSASLSFVDMPPRARSAYDSCVELLDDSVDALSRALSSVVS-----SSAKPQDVTTWLSAALTNHDTCTEGFDGVDDG---GVKDHMTAALQNLSELVSNCLAIFSASHDGDDFAGVPIQNRRLLGVEEREEKFPRWMRPKEREILEMPVSQIQADIIVSKDGNGTCKTISEAIKKAPQNSTRRIIIYVKAGRYEENNLKVGRKKINLMFVGDGKGKTVISGGKSIFDNITTFHTASFAATGAGFIARDITFENWAGPAKHQAVALRIGADHAVIYRCNIIGYQDTLYVHSNRQFFRECDIYGTVDFIFGNAAVVLQNCSIYARKPMDFQKNTITAQNRKDPNQNTGISIHASRVLAASDLQATNGSTQTYLGRPWKLFSRTVYMMSYIGGHVHTRGWLEWNTTFA-LDTLYYGEYLNSGPGSGLGQRVSWPGYRVINSTAEANRFTVAEFIYGSSWLPSTGVSFLAGL 596          

BLAST of Vfaba.Hedin2.R1.4g055160.1 vs. Araport11
Match: AT5G27870.1 (| Plant invertase/pectin methylesterase inhibitor superfamily | Chr5:9878991-9881806 REVERSE LENGTH=732 | 201606)

HSP 1 Score: 412.149 bits (1058), Expect = 1.778e-135
Identity = 225/576 (39.06%), Postives = 341/576 (59.20%), Query Frame = 0

Query:   10 YGKVDELEQQAFKKKTRKRITIIIISSLILIAVIVAAVAGILIHKRNTSSNPSPNSLPNTDLNPATSLKSVCDATQYPHSCIAAISSLPESNTTDPEKLFKLSLKVAMDELSNL---TRLKLKASGDTRLEKAIAVCSSVLEDSLDRLNDSMSMIIADDKILSPAKVRDVETWITAALTDHDTCLDAVGEVNSPAARGVLTQIETAMRNSTEFASNSLAIVSKVIRLLSNFEIPS-HHRRLL--EFPEWLGTAERRLL-ADSVNMTVDAVVAKDGSGQYTTIGEALQNVKKKSDVRFVVYVKKGIYVENIDLDKNTWNVMIYGDGMTETIVSGSRNFIDGTPTFETATFAVKGKGFIAKDIQFVNTAGASKHQAVAFRSGSDQSVFYKCSFVGYQDTLYAHSNRQFYRECDITGTIDFIFGNAAVVFQNCKIMPRQPLSNQFNTITAQGKKDPNQNSGIVIQKSTITPYDNLVA-----PTYLGRPWKDFSTTLIMKSEIGSFLKPVGWISWVANVEPPSSILYAEYQNTGPGADVTQRVKWVGYKPAIADEEASKFTVESFIQGSEWLPNAAVQF 573
            YG  DE        K +KR  II ISS++LI+++VA   G+ ++K + + +    +          ++K VC  T Y  +C   +      +T+DP +L K +    M ++S++   ++  ++   D R + A+  C  +++ ++  L+ S   +   +       +  +  W++A ++   TCLD        A       I+ A++ + +   N LA+V+++   L   +IP  + RRLL  EFP W+    RRLL A    +  D VVA+DGSGQY TI EAL  V KK +  FVV++K+GIY E + ++++  +++  GDG  +T++SGS+++ DG  T++TAT A+ G  FIAK+I F NTAGA KHQAVA R  +D+S+FY C F GYQDTLYAHS+RQFYR+C I+GTIDF+FG+AA VFQNC ++ R+PL NQ   ITA G+KDP +++G V+Q  TI    + +A      TYLGRPWK++S T+IM + I  F+ P GW  W+      +++ Y+E QNTGPGA +T+RV W G K  ++DEE  KFT   +IQG  W+P   V +
Sbjct:    3 YGYDDE------DAKRKKRYVIISISSVLLISMVVAVTIGVSVNKSDNAGDEEITT-------SVKAIKDVCAPTDYKETCEDTLRK-DAKDTSDPLELVKTAFNATMKQISDVAKKSQTMIELQKDPRAKMALDQCKELMDYAIGELSKSFEELGKFEFHKVDEALVKLRIWLSATISHEQTCLDGFQGTQGNAGE----TIKKALKTAVQLTHNGLAMVTEMSNYLGQMQIPEMNSRRLLSQEFPSWMDARARRLLNAPMSEVKPDIVVAQDGSGQYKTINEALNFVPKKKNTTFVVHIKEGIYKEYVQVNRSMTHLVFIGDGPDKTVISGSKSYKDGITTYKTATVAIVGDHFIAKNIAFENTAGAIKHQAVAIRVLADESIFYNCKFDGYQDTLYAHSHRQFYRDCTISGTIDFLFGDAAAVFQNCTLLVRKPLLNQACPITAHGRKDPRESTGFVLQGCTIVGEPDYLAVKEQSKTYLGRPWKEYSRTIIMNTFIPDFVPPEGWQPWLGEFG-LNTLFYSEVQNTGPGAAITKRVTWPGIK-KLSDEEILKFTPAQYIQGDAWIPGKGVPY 558          

BLAST of Vfaba.Hedin2.R1.4g055160.1 vs. Araport11
Match: AT3G49220.3 (| Plant invertase/pectin methylesterase inhibitor superfamily | Chr3:18249705-18253647 FORWARD LENGTH=643 | 201606)

HSP 1 Score: 408.297 bits (1048), Expect = 4.050e-135
Identity = 232/576 (40.28%), Postives = 330/576 (57.29%), Query Frame = 0

Query:   23 KKTRKRI---TIIIISSLILIAVIVAAVAGILIHKRNTSSNPSPNSLPNTDLNPATSLKSVCDATQYPHSCIAAISSLPESNTTDPEK-LFKLSLKVAMDELSNLTRLKLKAS---GDTRLEKAIAVCSSVLEDSLDRLNDSMSMIIADDKILSPAKVRDVETWITAALTDHDTCLDAVGEVNSPAARGVLTQIETAMRNSTEFASNSLAIVSKVIRLLSNFEIPSHHRRLL-------EFPEWLGTAERRLLADSVN-MTVDAVVAKDGSGQYTTIGEALQNVKKKSDVRFVVYVKKGIYVEN-IDLDKNTWNVMIYGDGMTETIVSGSRNFIDGTPTFETATFAVKGKGFIAKDIQFVNTAGASKHQAVAFRSGSDQSVFYKCSFVGYQDTLYAHSNRQFYRECDITGTIDFIFGNAAVVFQNCKIMPRQPLSNQFNTITAQGKKDPNQNSGIVIQKSTITPYDNLVA-----PTYLGRPWKDFSTTLIMKSEIGSFLKPVGWISWVANVEPPSSILYAEYQNTGPGADVTQRVKWVGYKPAIADEEASKFTVESFIQGSEWLPNAAVQFESTL 577
            ++++K++   +I++  SLIL A I A V   L  K N        S+P     P+ ++   C+ T++P  C+ ++   P S      K L  +++ + +   S+        S      R   A   C  +L+DS+D L+ ++S +++     S AK +DV TW++AALT+HDTC +    V+     GV   +  A++N +E  SN LAI S          +P  +RRLL       +FP W+   ER +L   V+ +  D +V+KDG+G   TI EA++   + S  R ++YVK G Y EN + + +   N+M  GDG  +T++SG ++  D   TF TA+FA  G GFIA+DI F N AG +KHQAVA R G+D +V Y+C+ +GYQDTLY HSNRQF+RECDI GT+DFIFGNAAVV QNC I  R+P+  Q NTITAQ +KDPNQN+GI I  S +    +L A      TYLGRPWK FS T+ M S IG  +   GW+ W        ++ Y EY N+GPG+ + QRV W GY+   +  EA++FTV  FI GS WLP+  V F + L
Sbjct:   84 RRSKKKLVVSSIVLAISLILAAAIFAGVRSRL--KLN-------QSVPGLARKPSQAISKACELTRFPELCVDSLMDFPGSLAASSSKDLIHVTVNMTLHHFSHALYSSASLSFVDMPPRARSAYDSCVELLDDSVDALSRALSSVVS-----SSAKPQDVTTWLSAALTNHDTCTEGFDGVDDG---GVKDHMTAALQNLSELVSNCLAIFSASHDGDDFAGVPIQNRRLLGVEEREEKFPRWMRPKEREILEMPVSQIQADIIVSKDGNGTCKTISEAIKKAPQNSTRRIIIYVKAGRYEENNLKVGRKKINLMFVGDGKGKTVISGGKSIFDNITTFHTASFAATGAGFIARDITFENWAGPAKHQAVALRIGADHAVIYRCNIIGYQDTLYVHSNRQFFRECDIYGTVDFIFGNAAVVLQNCSIYARKPMDFQKNTITAQNRKDPNQNTGISIHASRVLAASDLQATNGSTQTYLGRPWKLFSRTVYMMSYIGGHVHTRGWLEWNTTFA-LDTLYYGEYLNSGPGSGLGQRVSWPGYRVINSTAEANRFTVAEFIYGSSWLPSTGVSFLAGL 641