Vigst.05G166500.01
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Transcript Overview
Alignments
The following features are aligned
Analyses
This mRNA is derived from or has results from the following analyses Orthologs
Syntenic blocks Orthologs Relationships
This mRNA is a part of the following gene feature(s):
The following exon feature(s) are a part of this mRNA:
The following CDS feature(s) are a part of this mRNA:
The following polypeptide feature(s) derives from this mRNA:
Homology
BLAST of Vigst.05G166500.01 vs. ExPASy TrEMBL (2024)
Match: tr|A0A0R0GS00|A0A0R0GS00_SOYBN (Neutral ceramidase OS=Glycine max OX=3847 GN=100793262 PE=3 SV=1) HSP 1 Score: 1373.99 bits (3555), Expect = 0.000e+0 Identity = 648/746 (86.86%), Postives = 697/746 (93.43%), Query Frame = 0
Query: 9 MAENHVIVALGLLVCGWSWVQGTHGEYLIGVGSYDMTGPAADVNMMGYANMEQNTAGIHFRLRARTFIVGESPDGPRFVFVNLDACMASQLLTIKVLERLNSRFGKLYNEENVAISGTHTHGGPGGYMQYLVYSVTSLGFVKQSFDVIADAIEQSIIQAHNNLKPASIYLNIGEVKDGGINRSPSAYLQNPANERARYASNVDTQMTLLKFVDEESGESIGAFTWFATHGTSMTNKNKLITGDNKGVAARLFEDWFASQNTSSTTNSSVSDVGQLVKMAQSIKATRGKDCSKQASKASKVRKNDGSLFVAAFCQSNVGDVSPNVLGAFCIDTGKPCDFNHSSCNGNDQLCVGRGPGYPDEILSTKIIGERQFKSAVELFQSASEEVKGKIEYSHVYLNFTDIEVELESNKVVKTCPAALGPGFAAGTTDGPGAFGFQQGDLKINPFWKNLRDFLKEPSQYQVDCQNPKPVLLSTGEMFDPYAWAPAILPIQILRLGKLIILSVPGEFTTMAGRRLREAVKETLISSSNGEFNDETHVVIAGLTNTYSQYIATFEEYQHQRYEAASTLYGPHTLSAYIQEFKKLAQAMAEGKNITTKGPSPPDLSSVQISLLLGPFGDSPPAGIEFGDIKEDIAFPERGYFRKGDTPSATFWSSNPRYDLLTEGTFAAVERLQGERWIATYDDDDLSLFFKWKVDNSSMHGLATIEWEIPFGSVSGVYRLRHFGATRITITSPISYFTGASSAFAVQ 754
MA+N+VIVALGL+V W+W+QG HGEYLIGVGSYDMTGPAADVNMMGYAN QNTAGIHFRLRARTFIV ES GPRFVFVNLDA MASQLLTIKVLERLNSRFG LY EENVAISGTHTH GPGGY+QY+VYSVTSLGFVKQSFD IA+AIEQSIIQAHNNLKP SI++N G+VKD GINRSPSAYLQNPA+ERARY +NVDTQMTL++FVD SG++IGAF+WF THGTSM+N+NKLI+GDNKGVAARLFEDWFASQN S+ TNS+V D+GQL+K AQSIKATRGKDC K AS+ASKVRKNDGSLFV AFCQSNVGDVSPNVLGAFCID+GKPCDFN SSC+GNDQLCVGRGPGYPDEILSTKIIGERQFK+AV+LF+S SEE+ GKI+Y HVYLNFTDIEVEL+SNKVVKTCPAALGPGFAAGTTDGPG FGFQQGD KINPFWKN+RDFL +PSQYQVDCQNPKPVLLSTGEMF PY WAPAILPIQILRLGKLIILSVPGE TTMAGRRLREAVKETLISSSNGEF+DETHVVIAGLTNTYSQYIATFEEYQ QRYEAASTLYGPHTLSAYIQEFKKLAQAMAEG+NIT KGPSPPDLSSVQIS LL P G+SPP G++FGDIKED+AFP+RGYF KGDTPSATFWS+NPRYDLLTEGTFAAVERLQGERWI+ YDDDDLSLFFKWKVDNSS+HGLATIEWEIP +VSGVYRL+HFGATR TI SPI+YFTGASSAFAVQ
Sbjct: 1 MAQNYVIVALGLVVYAWTWMQGAHGEYLIGVGSYDMTGPAADVNMMGYANPLQNTAGIHFRLRARTFIVAESLQGPRFVFVNLDAGMASQLLTIKVLERLNSRFGNLYTEENVAISGTHTHAGPGGYLQYVVYSVTSLGFVKQSFDAIANAIEQSIIQAHNNLKPGSIFMNTGDVKDAGINRSPSAYLQNPADERARYPTNVDTQMTLMRFVDGASGKNIGAFSWFPTHGTSMSNQNKLISGDNKGVAARLFEDWFASQNNSTNTNSTVPDIGQLMKKAQSIKATRGKDCKKLASQASKVRKNDGSLFVGAFCQSNVGDVSPNVLGAFCIDSGKPCDFNRSSCHGNDQLCVGRGPGYPDEILSTKIIGERQFKTAVKLFESTSEELSGKIDYRHVYLNFTDIEVELDSNKVVKTCPAALGPGFAAGTTDGPGLFGFQQGDTKINPFWKNVRDFLTKPSQYQVDCQNPKPVLLSTGEMFYPYPWAPAILPIQILRLGKLIILSVPGELTTMAGRRLREAVKETLISSSNGEFDDETHVVIAGLTNTYSQYIATFEEYQQQRYEAASTLYGPHTLSAYIQEFKKLAQAMAEGENITIKGPSPPDLSSVQISFLLDPLGESPPKGVKFGDIKEDVAFPKRGYFTKGDTPSATFWSANPRYDLLTEGTFAAVERLQGERWISVYDDDDLSLFFKWKVDNSSLHGLATIEWEIPNDAVSGVYRLKHFGATRTTIISPINYFTGASSAFAVQ 746
BLAST of Vigst.05G166500.01 vs. ExPASy TrEMBL (2024)
Match: tr|A0A371GK45|A0A371GK45_MUCPR (Neutral ceramidase (Fragment) OS=Mucuna pruriens OX=157652 GN=NCER2 PE=3 SV=1) HSP 1 Score: 1372.45 bits (3551), Expect = 0.000e+0 Identity = 652/748 (87.17%), Postives = 697/748 (93.18%), Query Frame = 0
Query: 9 MAENHVIVALGLLVCGWSWVQGTHGEYLIGVGSYDMTGPAADVNMMGYANMEQNTAGIHFRLRARTFIVGESPDGPRFVFVNLDACMASQLLTIKVLERLNSRFGKLYNEENVAISGTHTHGGPGGYMQYLVYSVTSLGFVKQSFDVIADAIEQSIIQAHNNLKPASIYLNIGEVKDGGINRSPSAYLQNPANERARYASNVDTQMTLLKFVDEESGESIGAFTWFATHGTSMTNKNKLITGDNKGVAARLFEDWFASQNTSS--TTNSSVSDVGQLVKMAQSIKATRGKDCSKQASKASKVRKNDGSLFVAAFCQSNVGDVSPNVLGAFCIDTGKPCDFNHSSCNGNDQLCVGRGPGYPDEILSTKIIGERQFKSAVELFQSASEEVKGKIEYSHVYLNFTDIEVELESNKVVKTCPAALGPGFAAGTTDGPGAFGFQQGDLKINPFWKNLRDFLKEPSQYQVDCQNPKPVLLSTGEMFDPYAWAPAILPIQILRLGKLIILSVPGEFTTMAGRRLREAVKETLISSSNGEFNDETHVVIAGLTNTYSQYIATFEEYQHQRYEAASTLYGPHTLSAYIQEFKKLAQAMAEGKNITTKGPSPPDLSSVQISLLLGPFGDSPPAGIEFGDIKEDIAFPERGYFRKGDTPSATFWSSNPRYDLLTEGTFAAVERLQGERWIATYDDDDLSLFFKWKVDNSSMHGLATIEWEIPFGSVSGVYRLRHFGATRITITSPISYFTGASSAFAVQ 754
M +NHVIVAL L VCGWSW+QGTHGEYLIGVGSYDMTGPAADVNMMGYAN EQNTAGIHFRLRARTFIV E+ GPRFVFVNLDA MASQLLTIKVLERL SRFG LY EENVAISGTHTH GPGGY+QY+VYSVTSLGFVKQSFD IA+AIEQSIIQAHNNLKP S+++N G+VKD GINRSPSAYLQNPA ERARY +NVDTQM+LL+FVD SG++IGAF+WFATHGTSM+N+NKLI+G NKGVAARLFEDWFASQN SS TNS V D+GQLVK AQSIKAT GKDC++Q S+ASKVRKNDGSLFV AFCQSNVGDVSPNVLGAFCID+GKPCDFNHSSCNGNDQLCVGRGPGYPDEILSTK+IGERQFK+AVELF+SASEE+ GKI+Y HVYLNFTDIEVEL+SNKV+KTCPAALGPGFAAGTTDGPG FGFQQGD +INPFWKN+RDFLKEPSQYQVDCQNPKPVLLSTGEMFDPY WAPAILPIQILRLGKLIILSVPGEFTTMAGRRLREAVKETL+SSSNGEFN+ THVVIAGLTNTYSQYIATFEEYQHQRYEAASTLYGPHTLSAYIQEFKKLA AMA G+NIT +GPSPPDLSSVQISLL GPFGDSPP GI+FGDIKEDI+F E+GYF KGD PSATFWS+NPRYDLLTEGTFAAVERLQ ERWIA YDDDDLSLFFKWKVDNSS+H LATIEWEIP +VSGVYRL+HFGATRITI SPI+YFTGASSAFAVQ
Sbjct: 1 MGQNHVIVALSL-VCGWSWMQGTHGEYLIGVGSYDMTGPAADVNMMGYANFEQNTAGIHFRLRARTFIVAENLQGPRFVFVNLDAGMASQLLTIKVLERLTSRFGNLYTEENVAISGTHTHAGPGGYLQYVVYSVTSLGFVKQSFDAIANAIEQSIIQAHNNLKPGSVFMNRGDVKDAGINRSPSAYLQNPAEERARYPTNVDTQMSLLRFVDGASGKNIGAFSWFATHGTSMSNENKLISGGNKGVAARLFEDWFASQNKSSPSNTNSKVPDIGQLVKKAQSIKATGGKDCNQQTSQASKVRKNDGSLFVGAFCQSNVGDVSPNVLGAFCIDSGKPCDFNHSSCNGNDQLCVGRGPGYPDEILSTKMIGERQFKTAVELFESASEELAGKIDYRHVYLNFTDIEVELDSNKVIKTCPAALGPGFAAGTTDGPGVFGFQQGDTEINPFWKNVRDFLKEPSQYQVDCQNPKPVLLSTGEMFDPYPWAPAILPIQILRLGKLIILSVPGEFTTMAGRRLREAVKETLMSSSNGEFNNATHVVIAGLTNTYSQYIATFEEYQHQRYEAASTLYGPHTLSAYIQEFKKLALAMATGENITVQGPSPPDLSSVQISLLFGPFGDSPPEGIKFGDIKEDISFLEKGYFTKGDAPSATFWSANPRYDLLTEGTFAAVERLQEERWIAVYDDDDLSLFFKWKVDNSSLHALATIEWEIPNDAVSGVYRLKHFGATRITIISPITYFTGASSAFAVQ 747
BLAST of Vigst.05G166500.01 vs. ExPASy TrEMBL (2024)
Match: tr|A0A445HCL7|A0A445HCL7_GLYSO (Neutral ceramidase OS=Glycine soja OX=3848 GN=D0Y65_036012 PE=3 SV=1) HSP 1 Score: 1360.9 bits (3521), Expect = 0.000e+0 Identity = 647/757 (85.47%), Postives = 696/757 (91.94%), Query Frame = 0
Query: 9 MAENHVIVALGLLVCGWSWVQGTHGEYLIGVGSYDMTGPAADVNMMGYANMEQNTAGIHFRLRARTFIVGESPDGPRFVFVNLDACMASQLLTIKVLERLNSRFGKLYNEENVAISGTHTHGGPGGYMQYLVYSVTSLGFVKQSFDVIADAIEQSIIQAHNNLKPASIYLNIGEVKDGGINRSPSAYLQNPANERARYASNVDTQMTLLKFVDEESGESIGAFTWFATHGTSMTNKNKLITGDNKGVAARLFEDWFASQNTSSTTNSSVSDVGQLVKMAQSIKATRGKDCSKQASKASKVRKNDGSLFVAAFCQSNVGDVSPNVLGAFCIDTGKPCDFNHSSCNGNDQLCVGRGPG-----------YPDEILSTKIIGERQFKSAVELFQSASEEVKGKIEYSHVYLNFTDIEVELESNKVVKTCPAALGPGFAAGTTDGPGAFGFQQGDLKINPFWKNLRDFLKEPSQYQVDCQNPKPVLLSTGEMFDPYAWAPAILPIQILRLGKLIILSVPGEFTTMAGRRLREAVKETLISSSNGEFNDETHVVIAGLTNTYSQYIATFEEYQHQRYEAASTLYGPHTLSAYIQEFKKLAQAMAEGKNITTKGPSPPDLSSVQISLLLGPFGDSPPAGIEFGDIKEDIAFPERGYFRKGDTPSATFWSSNPRYDLLTEGTFAAVERLQGERWIATYDDDDLSLFFKWKVDNSSMHGLATIEWEIPFGSVSGVYRLRHFGATRITITSPISYFTGASSAFAVQ 754
MA+N+VIVALGL+V W+W+QG HGEYLIGVGSYDMTGPAADVNMMGYAN QNTAGIHFRLRARTFIV ES GPRFVFVNLDA MASQLLTIKVLERLNSRFG LY EENVAISGTHTH GPGGY+QY+VYSVTSLGFVKQSFD IA+AIEQSIIQAHNNLKP SI++N G+VKD GINRSPSAYLQNPA+ERARY +NVDTQMTL++FVD SG++IGAF+WF THGTSM+N+NKLI+GDNKGVAARLFEDWFASQN S+ TNS+V D+GQL+K AQSIKATRGKDC K AS+ASKVRKNDGSLFV AFCQSNVGDVSPNVLGAFCID+GKPCDFN SSC+GNDQLCVGRGPG YPDEILSTKIIGERQFK+AV+LF+S SEE+ GKI+Y HVYLNFTDIEVEL+SNKVVKTCPAALGPGFAAGTTDGPG FGFQQGD KINPFWKN+RDFL +PSQYQVDCQNPKPVLLSTGEMF PY WAPAILPIQILRLGKLIILSVPGE TTMAGRRLREAVKETL SSSNGEF+DETHVVIAGLTNTYSQYIATFEEYQ QRYEAASTLYGPHTLSAYIQEFKKLAQAMAEG+NIT KGPSPPDLSSVQIS LL P G+SPP G++FGDIKED+AFP+RGYF KGDTPSATFWS+NPRYDLLTEGTFAAVERLQGERWI+ YDDDDLSLFFKWKVDNSS+HGLATIEWEIP +VSGVYRL+HFGATR TI SPI+YFTGASSAFAVQ
Sbjct: 1 MAQNYVIVALGLVVYAWTWMQGAHGEYLIGVGSYDMTGPAADVNMMGYANPLQNTAGIHFRLRARTFIVAESLQGPRFVFVNLDAGMASQLLTIKVLERLNSRFGNLYTEENVAISGTHTHAGPGGYLQYVVYSVTSLGFVKQSFDAIANAIEQSIIQAHNNLKPGSIFMNTGDVKDAGINRSPSAYLQNPADERARYPTNVDTQMTLMRFVDGASGKNIGAFSWFPTHGTSMSNQNKLISGDNKGVAARLFEDWFASQNNSTNTNSTVPDIGQLMKKAQSIKATRGKDCKKLASQASKVRKNDGSLFVGAFCQSNVGDVSPNVLGAFCIDSGKPCDFNRSSCHGNDQLCVGRGPGKGRAYNFAWSRYPDEILSTKIIGERQFKTAVKLFESTSEELSGKIDYRHVYLNFTDIEVELDSNKVVKTCPAALGPGFAAGTTDGPGLFGFQQGDTKINPFWKNVRDFLTKPSQYQVDCQNPKPVLLSTGEMFYPYPWAPAILPIQILRLGKLIILSVPGELTTMAGRRLREAVKETLKSSSNGEFDDETHVVIAGLTNTYSQYIATFEEYQQQRYEAASTLYGPHTLSAYIQEFKKLAQAMAEGENITIKGPSPPDLSSVQISFLLDPLGESPPKGVKFGDIKEDVAFPKRGYFTKGDTPSATFWSANPRYDLLTEGTFAAVERLQGERWISVYDDDDLSLFFKWKVDNSSLHGLATIEWEIPNDAVSGVYRLKHFGATRTTIISPINYFTGASSAFAVQ 757
BLAST of Vigst.05G166500.01 vs. ExPASy TrEMBL (2024)
Match: tr|A0A0B2S6C2|A0A0B2S6C2_GLYSO (Neutral ceramidase OS=Glycine soja OX=3848 GN=glysoja_039960 PE=3 SV=1) HSP 1 Score: 1343.18 bits (3475), Expect = 0.000e+0 Identity = 635/727 (87.35%), Postives = 680/727 (93.54%), Query Frame = 0
Query: 28 VQGTHGEYLIGVGSYDMTGPAADVNMMGYANMEQNTAGIHFRLRARTFIVGESPDGPRFVFVNLDACMASQLLTIKVLERLNSRFGKLYNEENVAISGTHTHGGPGGYMQYLVYSVTSLGFVKQSFDVIADAIEQSIIQAHNNLKPASIYLNIGEVKDGGINRSPSAYLQNPANERARYASNVDTQMTLLKFVDEESGESIGAFTWFATHGTSMTNKNKLITGDNKGVAARLFEDWFASQNTSSTTNSSVSDVGQLVKMAQSIKATRGKDCSKQASKASKVRKNDGSLFVAAFCQSNVGDVSPNVLGAFCIDTGKPCDFNHSSCNGNDQLCVGRGPGYPDEILSTKIIGERQFKSAVELFQSASEEVKGKIEYSHVYLNFTDIEVELESNKVVKTCPAALGPGFAAGTTDGPGAFGFQQGDLKINPFWKNLRDFLKEPSQYQVDCQNPKPVLLSTGEMFDPYAWAPAILPIQILRLGKLIILSVPGEFTTMAGRRLREAVKETLISSSNGEFNDETHVVIAGLTNTYSQYIATFEEYQHQRYEAASTLYGPHTLSAYIQEFKKLAQAMAEGKNITTKGPSPPDLSSVQISLLLGPFGDSPPAGIEFGDIKEDIAFPERGYFRKGDTPSATFWSSNPRYDLLTEGTFAAVERLQGERWIATYDDDDLSLFFKWKVDNSSMHGLATIEWEIPFGSVSGVYRLRHFGATRITITSPISYFTGASSAFAVQ 754
+QG HGEYLIGVGSYDMTGPAADVNMMGYAN QNTAGIHFRLRARTFIV ES GPRFVFVNLDA MASQLLTIKVLERLNSRFG LY EENVAISGTHTH GPGGY+QY+VYSVTSLGFVKQSFD IA+AIEQSIIQAHNNLKP SI++N G+VKD GINRSPSAYLQNPA+ERARY +NVDTQMTL++FVD SG++IGAF+WF THGTSM+N+NKLI+GDNKGVAARLFEDWFASQN S+ TNS+V D+GQL+K AQSIKATRGKDC K AS+ASKVRKNDGSLFV AFCQSNVGDVSPNVLGAFCID+GKPCDFN SSC+GNDQLCVGRGPGYPDEILSTKIIGERQFK+AV+LF+S SEE+ GKI+Y HVYLNFTDIEVEL+SNKVVKTCPAALGPGFAAGTTDGPG FGFQQGD KINPFWKN+RDFL +PSQYQVDCQNPKPVLLSTGEMF PY WAPAILPIQILRLGKLIILSVPGE TTMAGRRLREAVKETLISSSNGEF+DETHVVIAGLTNTYSQYIATFEEYQ QRYEAASTLYGPHTLSAYIQEFKKLAQAMAEG+NIT KGPSPPDLSSVQIS LL P G+SPP G++FGDIKED+AFP+RGYF KGDTPSATFWS+NPRYDLLTEGTFAAVERLQGERWI+ YDDDDLSLFFKWKVDNSS+HGLATIEWEIP +VSGVYRL+HFGATR TI SPI+YFTGASSAFAVQ
Sbjct: 1 MQGAHGEYLIGVGSYDMTGPAADVNMMGYANPLQNTAGIHFRLRARTFIVAESLQGPRFVFVNLDAGMASQLLTIKVLERLNSRFGNLYTEENVAISGTHTHAGPGGYLQYVVYSVTSLGFVKQSFDAIANAIEQSIIQAHNNLKPGSIFMNTGDVKDAGINRSPSAYLQNPADERARYPTNVDTQMTLMRFVDGASGKNIGAFSWFPTHGTSMSNQNKLISGDNKGVAARLFEDWFASQNNSTNTNSTVPDIGQLMKKAQSIKATRGKDCKKLASQASKVRKNDGSLFVGAFCQSNVGDVSPNVLGAFCIDSGKPCDFNRSSCHGNDQLCVGRGPGYPDEILSTKIIGERQFKTAVKLFESTSEELSGKIDYRHVYLNFTDIEVELDSNKVVKTCPAALGPGFAAGTTDGPGLFGFQQGDTKINPFWKNVRDFLTKPSQYQVDCQNPKPVLLSTGEMFYPYPWAPAILPIQILRLGKLIILSVPGELTTMAGRRLREAVKETLISSSNGEFDDETHVVIAGLTNTYSQYIATFEEYQQQRYEAASTLYGPHTLSAYIQEFKKLAQAMAEGENITIKGPSPPDLSSVQISFLLDPLGESPPKGVKFGDIKEDVAFPKRGYFTKGDTPSATFWSANPRYDLLTEGTFAAVERLQGERWISVYDDDDLSLFFKWKVDNSSLHGLATIEWEIPNDAVSGVYRLKHFGATRTTIISPINYFTGASSAFAVQ 727
BLAST of Vigst.05G166500.01 vs. ExPASy TrEMBL (2024)
Match: tr|V7BM04|V7BM04_PHAVU (Neutral ceramidase OS=Phaseolus vulgaris OX=3885 GN=PHAVU_006G010200g PE=3 SV=1) HSP 1 Score: 1340.48 bits (3468), Expect = 0.000e+0 Identity = 648/746 (86.86%), Postives = 679/746 (91.02%), Query Frame = 0
Query: 9 MAENHVIVALGLLVCGWSWVQGTHGEYLIGVGSYDMTGPAADVNMMGYANMEQNTAGIHFRLRARTFIVGESPDGPRFVFVNLDACMASQLLTIKVLERLNSRFGKLYNEENVAISGTHTHGGPGGYMQYLVYSVTSLGFVKQSFDVIADAIEQSIIQAHNNLKPASIYLNIGEVKDGGINRSPSAYLQNPANERARYASNVDTQMTLLKFVDEESGESIGAFTWFATHGTSMTNKNKLITGDNKGVAARLFEDWFASQNTSSTTNSSVSDVGQLVKMAQSIKATRGKDCSKQASKASKVRKNDGSLFVAAFCQSNVGDVSPNVLGAFCIDTGKPCDFNHSSCNGNDQLCVGRGPGYPDEILSTKIIGERQFKSAVELFQSASEEVKGKIEYSHVYLNFTDIEVELESNKVVKTCPAALGPGFAAGTTDGPGAFGFQQGDLKINPFWKNLRDFLKEPSQYQVDCQNPKPVLLSTGEMFDPYAWAPAILPIQILRLGKLIILSVPGEFTTMAGRRLREAVKETLISSSNGEFNDETHVVIAGLTNTYSQYIATFEEYQHQRYEAASTLYGPHTLSAYIQEFKKLAQAMAEGKNITTKGPSPPDLSSVQISLLLGPFGDSPPAGIEFGDIKEDIAFPERGYFRKGDTPSATFWSSNPRYDLLTEGTFAAVERLQGERWIATYDDDDLSLFFKWKVDNSSMHGLATIEWEIPFGSVSGVYRLRHFGATRITITSPISYFTGASSAFAVQ 754
M E+ VIVALGLLVC WSWV GT GEYLIGVGSYDM+GPAADVNMMGYAN+EQNTAGIHFRLRARTFIVGE +GPRFVFVNLDA MASQLLTIKVL+RLNSRFG LY EENVAISGTHTH GPGGY+QYLVYSVTSLGFVKQSFDVIADAIEQSIIQAHNNLKP SI +NIG+VKD GINRSPSAYLQNPA ERARY SNVDTQMTLL+FVD ESG+SIGAF WFATHGTSM+N+NKLI+GDNK D+GQLVK AQSI AT GKDC+KQA++ SKVRKNDGSLFV AFCQSNVGDVSPNVLGAFC DTGKPCDFNHSSCNGNDQLCVGRGPGYP+EILST +IG RQFKSAVELFQSASEE+ GKIEY HVYLNFTDIEVEL+ NKVVKTCPAALGPGFAAGTTDGPGAFGFQQGDLKINPFWKNLRDFLKEPSQYQVDCQN KPVLLS GEMFDPY WAPAILPIQILRLGKLIILSVPGEFTTMAGRRLREAVKETLISSSNGEFND+THVVIAGLTNTYSQYIATFEEYQHQRYEAASTLYGPHTLSAYIQEFKKLA+AMAEG+NIT KGPSPPDLSSVQ SLLLGPFGDS P G +FGDIKEDI FPERGYFRKGDTPSATFWS+NPRYDLLTEGTFAAVERLQGERWIA YDDDDL+LFFKWKVDNSS+HGLA +EWEIP +VSGVYRLRHFGATRITITSPI+YFTGASSAFAVQ
Sbjct: 1 MGESGVIVALGLLVCAWSWVGGTQGEYLIGVGSYDMSGPAADVNMMGYANLEQNTAGIHFRLRARTFIVGEGLEGPRFVFVNLDAGMASQLLTIKVLQRLNSRFGNLYTEENVAISGTHTHAGPGGYLQYLVYSVTSLGFVKQSFDVIADAIEQSIIQAHNNLKPGSILINIGDVKDAGINRSPSAYLQNPAKERARYPSNVDTQMTLLRFVDGESGKSIGAFNWFATHGTSMSNENKLISGDNK-------------------------DIGQLVKKAQSINATGGKDCNKQANQESKVRKNDGSLFVGAFCQSNVGDVSPNVLGAFCTDTGKPCDFNHSSCNGNDQLCVGRGPGYPNEILSTNMIGGRQFKSAVELFQSASEELSGKIEYLHVYLNFTDIEVELD-NKVVKTCPAALGPGFAAGTTDGPGAFGFQQGDLKINPFWKNLRDFLKEPSQYQVDCQNSKPVLLSIGEMFDPYPWAPAILPIQILRLGKLIILSVPGEFTTMAGRRLREAVKETLISSSNGEFNDKTHVVIAGLTNTYSQYIATFEEYQHQRYEAASTLYGPHTLSAYIQEFKKLAKAMAEGQNITIKGPSPPDLSSVQRSLLLGPFGDSLPEGTKFGDIKEDIDFPERGYFRKGDTPSATFWSANPRYDLLTEGTFAAVERLQGERWIAAYDDDDLTLFFKWKVDNSSLHGLAILEWEIPDAAVSGVYRLRHFGATRITITSPINYFTGASSAFAVQ 720
BLAST of Vigst.05G166500.01 vs. ExPASy TrEMBL (2024)
Match: tr|A0A151QQ37|A0A151QQ37_CAJCA (Neutral ceramidase OS=Cajanus cajan OX=3821 GN=KK1_046911 PE=3 SV=1) HSP 1 Score: 1310.43 bits (3390), Expect = 0.000e+0 Identity = 630/735 (85.71%), Postives = 675/735 (91.84%), Query Frame = 0
Query: 28 VQGTHGEYLIGVGSYDMTGPAADVNMMGYANMEQNTAGIHFRLRARTFIVGESPDGPRFVFVNLDACMASQLLTIKVLERLNSRFGKLYNEENVAISGTHTHGGPGGYMQYLVYSVTSLGFVKQSFDVIADAIEQSIIQAHNNLKPASIYLNIGEVKDGGINRSPSAYLQNPANERARYASNVDTQMTLLKFVDEESGESIGAFTWFATHGTSMTNKNKLITGDNKGVAARLFEDWFASQNTSSTTN--------SSVSDVGQLVKMAQSIKATRGKDCSKQASKASKVRKNDGSLFVAAFCQSNVGDVSPNVLGAFCIDTGKPCDFNHSSCNGNDQLCVGRGPGYPDEILSTKIIGERQFKSAVELFQSASEEVKGKIEYSHVYLNFTDIEVELESNKVVKTCPAALGPGFAAGTTDGPGAFGFQQGDLKINPFWKNLRDFLKEPSQYQVDCQNPKPVLLSTGEMFDPYAWAPAILPIQILRLGKLIILSVPGEFTTMAGRRLREAVKETLISSSNGEFNDETHVVIAGLTNTYSQYIATFEEYQHQRYEAASTLYGPHTLSAYIQEFKKLAQAMAEGKNITTKGPSPPDLSSVQISLLLGPFGDSPPAGIEFGDIKEDIAFPERGYFRKGDTPSATFWSSNPRYDLLTEGTFAAVERLQGERWIATYDDDDLSLFFKWKVDNSSMHGLATIEWEIPFGSVSGVYRLRHFGATRITITSPISYFTGASSAFAVQ 754
+QGTHGEYLIGVGSYDMTGPAADVNMMGYANMEQNTAGIHFRLRARTFIV ES GPRFVFVNLDA MASQLLTIKVLERL+SRFG LY E+NVAISGTHTH GPGGY+QY+VYSVTSLGFV+QSFD IA+AIEQSIIQAHNNLKP SI++NIG+VKD GINRSPSAYLQNPA ERARY +NVDTQMTLL+F+D SG+SIGAF+WFATHGTSM+ NKLI+GDNKGVAARLFEDWFASQN SS++N + D+ QLVK+A+SIK T GKDC+++AS+ASKVRKNDGSLFV AFCQSNVGDVSPNVLGAFCID GKPCDFNHSSCNGNDQLCVGRGPGYPDEILSTKIIGERQFK+AVELF+SAS E+ GKI+Y HVYLNFTDIEV+L+SNKVVKTCPAALGPGFAAGTTDGPGAFGFQQGD +INPFWK LRD LKEPSQYQVDCQNPK VLLSTGEMFDPY WAPAILPIQI+RLGKLIILSVPGEFTTMAGRRLREAVKETLISSSNGEFN+ETHVVIAGLTNTYSQYIATFEEY+ QRYEAASTLYGPHTLSAYIQEFKKLA+AM EG NI KGPSPPDLSSVQI LLLGPFGDSPP GI+FGDIKEDI+FP+ GYF KGDT ATFWS+NPRYDLLTEGTFAAVERLQGERWIA YDDDDLSLF KWKVDNSS GLATIEWEIP +VSGVYRL+HFGATR+TI SPISYF GASSAFAVQ
Sbjct: 1 MQGTHGEYLIGVGSYDMTGPAADVNMMGYANMEQNTAGIHFRLRARTFIVAESLQGPRFVFVNLDAGMASQLLTIKVLERLHSRFGNLYTEQNVAISGTHTHAGPGGYLQYVVYSVTSLGFVEQSFDAIANAIEQSIIQAHNNLKPGSIFMNIGDVKDAGINRSPSAYLQNPAEERARYPTNVDTQMTLLRFMDGASGKSIGAFSWFATHGTSMSRDNKLISGDNKGVAARLFEDWFASQNKSSSSNTNSTEPGIKNFRDIRQLVKIARSIKPTGGKDCNRKASQASKVRKNDGSLFVGAFCQSNVGDVSPNVLGAFCIDNGKPCDFNHSSCNGNDQLCVGRGPGYPDEILSTKIIGERQFKTAVELFESASGELVGKIDYRHVYLNFTDIEVKLDSNKVVKTCPAALGPGFAAGTTDGPGAFGFQQGDTEINPFWKGLRDLLKEPSQYQVDCQNPKQVLLSTGEMFDPYPWAPAILPIQIIRLGKLIILSVPGEFTTMAGRRLREAVKETLISSSNGEFNNETHVVIAGLTNTYSQYIATFEEYKQQRYEAASTLYGPHTLSAYIQEFKKLARAMVEGINIPLKGPSPPDLSSVQIKLLLGPFGDSPPEGIKFGDIKEDISFPKEGYFTKGDTSCATFWSANPRYDLLTEGTFAAVERLQGERWIAVYDDDDLSLFLKWKVDNSSYQGLATIEWEIPNEAVSGVYRLKHFGATRVTILSPISYFVGASSAFAVQ 735
BLAST of Vigst.05G166500.01 vs. ExPASy TrEMBL (2024)
Match: tr|A0A1S2YC70|A0A1S2YC70_CICAR (Neutral ceramidase OS=Cicer arietinum OX=3827 GN=LOC101513807 PE=3 SV=1) HSP 1 Score: 1291.95 bits (3342), Expect = 0.000e+0 Identity = 611/747 (81.79%), Postives = 672/747 (89.96%), Query Frame = 0
Query: 9 MAENHVIVALGLLVCGWSWVQGTHGEYLIGVGSYDMTGPAADVNMMGYANMEQNTAGIHFRLRARTFIVGESPDGPRFVFVNLDACMASQLLTIKVLERLNSRFGKLYNEENVAISGTHTHGGPGGYMQYLVYSVTSLGFVKQSFDVIADAIEQSIIQAHNNLKPASIYLNIGEVKDGGINRSPSAYLQNPANERARYASNVDTQMTLLKFVDEESGESIGAFTWFATHGTSMTNKNKLITGDNKGVAARLFEDWFASQ-NTSSTTNSSVSDVGQLVKMAQSIKATRGKDCSKQASKASKVRKNDGSLFVAAFCQSNVGDVSPNVLGAFCIDTGKPCDFNHSSCNGNDQLCVGRGPGYPDEILSTKIIGERQFKSAVELFQSASEEVKGKIEYSHVYLNFTDIEVELESNKVVKTCPAALGPGFAAGTTDGPGAFGFQQGDLKINPFWKNLRDFLKEPSQYQVDCQNPKPVLLSTGEMFDPYAWAPAILPIQILRLGKLIILSVPGEFTTMAGRRLREAVKETLISSSNGEFNDETHVVIAGLTNTYSQYIATFEEYQHQRYEAASTLYGPHTLSAYIQEFKKLAQAMAEGKNITTKGPSPPDLSSVQISLLLGPFGDSPPAGIEFGDIKEDIAFPERGYFRKGDTPSATFWSSNPRYDLLTEGTFAAVERLQGERWIATYDDDDLSLFFKWKVDNSSMHGLATIEWEIPFGSVSGVYRLRHFGATRITITSPISYFTGASSAFAVQ 754
MA++ +I GL C W+W+Q THGEYLIGVGSYDMTGPAADVNMMGYAN+EQNTAGIHFRLRARTFIV E+ GPRFVFVNLDA MASQLLTIK+LERL SRFG LY EENVAISGTHTH GPGGY+QY+VYSVTSLGFVKQSFD +A+A+EQSIIQAHNNL+P SI++N G+VK+ INRSPSAYL NPA ERA+Y +NVDTQMTLLKFVD SG+S G+F+WFATHGTSM+ NKLI+GDNKGVAARLFEDWF SQ N+SS TN + D+G+LV+ A+SIKAT GK+CS++ S+ SKVRKNDGSLFV AFCQSNVGDVSPNVLGAFC D+GKPCDFNHSSCNGNDQLCVGRGPGYP+EILSTKIIGERQFKSAVELF SASEE+ GKI+Y HVYLNFTDIEVEL+SNKVVKTCPAALGP FAAGTTDGPG FGFQQGD +I+PFWK++RDFLKEPSQYQVDCQNPKPVLLS+GEMFDPY WAPAILPIQILRLGKLIILSVPGEFTTMAGRRLREAVKETLIS+SNGEF++ THVVIAGLTNTYSQYIAT EEY QRYEAASTLYGPHTLSAYIQEFKKLAQAMA+G I GPSPPDL SVQ S LLGPFGDS P GI+FGD KEDIAFP+ GYFRKGD PSATFWS+NPRYDLLTEGTFAAVERLQ ERWI+ +DDDDLSL F WKVDNSS+HGLA IEWEIP ++SGVYRL+HFGA++IT S I+YFTGASSAFAVQ
Sbjct: 1 MAQSLIIAVFGL-ACIWTWMQNTHGEYLIGVGSYDMTGPAADVNMMGYANIEQNTAGIHFRLRARTFIVAENLQGPRFVFVNLDAGMASQLLTIKLLERLKSRFGDLYTEENVAISGTHTHAGPGGYLQYVVYSVTSLGFVKQSFDAVANAVEQSIIQAHNNLEPGSIFINTGDVKEASINRSPSAYLLNPAEERAKYPANVDTQMTLLKFVDGASGKSTGSFSWFATHGTSMSRDNKLISGDNKGVAARLFEDWFTSQINSSSNTNFTEPDIGELVRQARSIKATGGKECSQKTSQTSKVRKNDGSLFVGAFCQSNVGDVSPNVLGAFCTDSGKPCDFNHSSCNGNDQLCVGRGPGYPNEILSTKIIGERQFKSAVELFGSASEELTGKIDYRHVYLNFTDIEVELDSNKVVKTCPAALGPAFAAGTTDGPGVFGFQQGDTEISPFWKSVRDFLKEPSQYQVDCQNPKPVLLSSGEMFDPYPWAPAILPIQILRLGKLIILSVPGEFTTMAGRRLREAVKETLISNSNGEFDNATHVVIAGLTNTYSQYIATIEEYHQQRYEAASTLYGPHTLSAYIQEFKKLAQAMAKGDKINGNGPSPPDLLSVQKSFLLGPFGDSTPDGIKFGDTKEDIAFPQSGYFRKGDRPSATFWSANPRYDLLTEGTFAAVERLQSERWISVHDDDDLSLLFTWKVDNSSLHGLAIIEWEIPKDAISGVYRLKHFGASKITTVSSINYFTGASSAFAVQ 746
BLAST of Vigst.05G166500.01 vs. ExPASy TrEMBL (2024)
Match: tr|A0A9D4WUD0|A0A9D4WUD0_PEA (Neutral ceramidase OS=Pisum sativum OX=3888 GN=KIW84_053276 PE=3 SV=1) HSP 1 Score: 1280.77 bits (3313), Expect = 0.000e+0 Identity = 606/748 (81.02%), Postives = 672/748 (89.84%), Query Frame = 0
Query: 9 MAENHVIVALGLLVCGWSWVQGTHGEYLIGVGSYDMTGPAADVNMMGYANMEQNTAGIHFRLRARTFIVGESPDGPRFVFVNLDACMASQLLTIKVLERLNSRFGKLYNEENVAISGTHTHGGPGGYMQYLVYSVTSLGFVKQSFDVIADAIEQSIIQAHNNLKPASIYLNIGEVKDGGINRSPSAYLQNPANERARYASNVDTQMTLLKFVDEESGESIGAFTWFATHGTSMTNKNKLITGDNKGVAARLFEDWFASQNTS--STTNSSVSDVGQLVKMAQSIKATRGKDCSKQASKASKVRKNDGSLFVAAFCQSNVGDVSPNVLGAFCIDTGKPCDFNHSSCNGNDQLCVGRGPGYPDEILSTKIIGERQFKSAVELFQSASEEVKGKIEYSHVYLNFTDIEVELESNKVVKTCPAALGPGFAAGTTDGPGAFGFQQGDLKINPFWKNLRDFLKEPSQYQVDCQNPKPVLLSTGEMFDPYAWAPAILPIQILRLGKLIILSVPGEFTTMAGRRLREAVKETLISSSNGEFNDETHVVIAGLTNTYSQYIATFEEYQHQRYEAASTLYGPHTLSAYIQEFKKLAQAMAEGKNITTKGPSPPDLSSVQISLLLGPFGDSPPAGIEFGDIKEDIAFPERGYFRKGDTPSATFWSSNPRYDLLTEGTFAAVERLQGERWIATYDDDDLSLFFKWKVDNSSMHGLATIEWEIPFGSVSGVYRLRHFGATRITITSPISYFTGASSAFAVQ 754
MA+ +I L L C W W+Q T+GEYLIGVGSYDMTGPAADVNMMGYA++EQNTAGIHFRLRARTFIV ++ GPRFVFVNLDA MASQLLTIK+LERL SRFG LY EENVAISGTHTH GPGGY+QY+VYSVTSLGFVKQSFD IA+A+EQSI+QAHNNLKP S+++N G+VK+ INRSPSAYL NPA ER+RY SN+DTQMTLLKFVD SGES GAF+WFATHGTSM+ NKLI+GDNKGVAARLFEDWF SQN S +NS+ D+G+LVK A++IKAT GK C+++ S+ASK+RKNDGSLFV AFCQSNVGDVSPNVLGAFC D+GKPCDFNHSSCNGND LCVGRGPG+PDEILSTKIIG+RQFKSAVELF+SASEE+ GKI+Y HVYLNFTDIEVEL+SNKVVKTCPAALGP FAAGTTDGPG FGFQQGD +I+PFWKN+RDFLK+PSQYQVDCQNPKPVLLS+GEMFDPY WAPAILPIQILRLGKLIILSVPGEFTTMAGRRLREAVKETLIS+S+GEFN+ETHVVIAGLTNTYSQYIATFEEY QRYEAASTLYGPHTLSAYIQEFKKLAQAMA+G I GPSPPDL SVQ S LLGPFGDS P GI+FGD+KEDIAFP+ GYF KGD PSATFWS+NPRYDLLTEGTFA+VERLQ ERWI+ +DDDDLSL FKWKVDN+S HGLATIEWEIP ++SGVYRL+HFGA++ TI SP +YFTGAS+AFAVQ
Sbjct: 1 MAQIPIITILSFL-CLWIWMQTTNGEYLIGVGSYDMTGPAADVNMMGYASIEQNTAGIHFRLRARTFIVADNLQGPRFVFVNLDAGMASQLLTIKLLERLKSRFGNLYTEENVAISGTHTHAGPGGYLQYVVYSVTSLGFVKQSFDAIANAVEQSIVQAHNNLKPGSVFVNTGDVKEASINRSPSAYLLNPAEERSRYPSNIDTQMTLLKFVDGASGESKGAFSWFATHGTSMSKNNKLISGDNKGVAARLFEDWFTSQNRSLSPNSNSTEPDIGELVKKARTIKATGGKGCNQKTSQASKLRKNDGSLFVGAFCQSNVGDVSPNVLGAFCADSGKPCDFNHSSCNGNDLLCVGRGPGFPDEILSTKIIGDRQFKSAVELFESASEELTGKIDYRHVYLNFTDIEVELDSNKVVKTCPAALGPAFAAGTTDGPGVFGFQQGDTEISPFWKNVRDFLKKPSQYQVDCQNPKPVLLSSGEMFDPYPWAPAILPIQILRLGKLIILSVPGEFTTMAGRRLREAVKETLISNSDGEFNNETHVVIAGLTNTYSQYIATFEEYHQQRYEAASTLYGPHTLSAYIQEFKKLAQAMAKGAKINGNGPSPPDLLSVQKSFLLGPFGDSTPDGIKFGDMKEDIAFPQSGYFTKGDKPSATFWSANPRYDLLTEGTFASVERLQEERWISVHDDDDLSLLFKWKVDNTSFHGLATIEWEIPKDAISGVYRLKHFGASKTTIASPTNYFTGASNAFAVQ 747
BLAST of Vigst.05G166500.01 vs. ExPASy TrEMBL (2024)
Match: tr|G7J608|G7J608_MEDTR (Neutral ceramidase OS=Medicago truncatula OX=3880 GN=11438492 PE=3 SV=1) HSP 1 Score: 1271.14 bits (3288), Expect = 0.000e+0 Identity = 611/748 (81.68%), Postives = 672/748 (89.84%), Query Frame = 0
Query: 9 MAENHVIVALGLLVCGWSWVQGTHGEYLIGVGSYDMTGPAADVNMMGYANMEQNTAGIHFRLRARTFIVGESPDGPRFVFVNLDACMASQLLTIKVLERLNSRFGKLYNEENVAISGTHTHGGPGGYMQYLVYSVTSLGFVKQSFDVIADAIEQSIIQAHNNLKPASIYLNIGEVKDGGINRSPSAYLQNPANERARYASNVDTQMTLLKFVDEESGESIGAFTWFATHGTSMTNKNKLITGDNKGVAARLFEDWFASQNTSSTTNSSVS--DVGQLVKMAQSIKATRGKDCSKQASKASKVRKNDGSLFVAAFCQSNVGDVSPNVLGAFCIDTGKPCDFNHSSCNGNDQLCVGRGPGYPDEILSTKIIGERQFKSAVELFQSASEEVKGKIEYSHVYLNFTDIEVELESNKVVKTCPAALGPGFAAGTTDGPGAFGFQQGDLKINPFWKNLRDFLKEPSQYQVDCQNPKPVLLSTGEMFDPYAWAPAILPIQILRLGKLIILSVPGEFTTMAGRRLREAVKETLISSSNGEFNDETHVVIAGLTNTYSQYIATFEEYQHQRYEAASTLYGPHTLSAYIQEFKKLAQAMAEGKNITTKGPSPPDLSSVQISLLLGPFGDSPPAGIEFGDIKEDIAFPERGYFRKGDTPSATFWSSNPRYDLLTEGTFAAVERLQGERWIATYDDDDLSLFFKWKVDNSSMHGLATIEWEIPFGSVSGVYRLRHFGATRITITSPISYFTGASSAFAVQ 754
MA+ +I LGL VC WSW+Q T+GEYLIGVGSYDMTGPAADVNMMGYAN+EQNTAGIHFRLRARTFIV E+ GPRFVFVNLDA MASQLLTIK+LERL SRFG LY EENVAISG HTH GPGGY+QY+VYSVTSLGFV QSFD IA+A+EQSIIQAHNNLKP SI++N G+VK+ INRSPSAYL NPA ER+RY SNVDTQMTLLKFVD SG+S G+F+WFATHGTSM+N NKLI+GDNKGVAARLFEDWF SQN SS+ NS+ + D+G+LV++AQ IKAT GKDC++++S+ASKVRKNDGSLFV AFCQSNVGDVSPNVLGAFCID+GKPCDFNHSSCNGND LCVGRGPGYP+EILSTKIIGERQF+SAVELF SASEE+ GKI+Y HVYLNFT+IEVEL++ KVVKTCPAALGPGFAAGTTDGPG FGFQQGD +I+PFWKN+RDFLKEPSQYQVDCQNPKPVLLS+GEMFDPY WAPAILPIQILRLGKLIILSVPGEFTTMAGRRLREAVKETLIS+SNGEFN+ETHVVIAGLTNTYSQYIATFEEY QRYEAASTLYGPHTLSAYIQEF KLAQAMA+G I G SPPDL SVQ S LL PFGD+ P GI+ GDIKEDIAFP GYF KGD PSATFWS+NPRYDLLTEGT+A VERLQGERWI+ DDDDLSLFF+WKVDN+S HG A IEWEIP ++SGVYRL+HFGA++ TI SPI+YFTGASSAFAVQ
Sbjct: 1 MAQRPIITVLGL-VCFWSWMQSTYGEYLIGVGSYDMTGPAADVNMMGYANIEQNTAGIHFRLRARTFIVAENLQGPRFVFVNLDAGMASQLLTIKLLERLKSRFGNLYTEENVAISGIHTHAGPGGYLQYVVYSVTSLGFVTQSFDAIANAVEQSIIQAHNNLKPGSIFINTGDVKEASINRSPSAYLLNPAEERSRYPSNVDTQMTLLKFVDSASGKSKGSFSWFATHGTSMSNNNKLISGDNKGVAARLFEDWFTSQNKSSSPNSNSTELDIGELVQIAQLIKATGGKDCNEKSSQASKVRKNDGSLFVGAFCQSNVGDVSPNVLGAFCIDSGKPCDFNHSSCNGNDLLCVGRGPGYPNEILSTKIIGERQFRSAVELFGSASEELTGKIDYRHVYLNFTNIEVELDNKKVVKTCPAALGPGFAAGTTDGPGVFGFQQGDPEISPFWKNVRDFLKEPSQYQVDCQNPKPVLLSSGEMFDPYPWAPAILPIQILRLGKLIILSVPGEFTTMAGRRLREAVKETLISNSNGEFNNETHVVIAGLTNTYSQYIATFEEYHQQRYEAASTLYGPHTLSAYIQEFNKLAQAMAKGDKIYGNGTSPPDLLSVQKSFLLDPFGDTTPDGIKLGDIKEDIAFPGSGYFTKGDKPSATFWSANPRYDLLTEGTYAVVERLQGERWISVQDDDDLSLFFRWKVDNTSFHGFAAIEWEIPTDAISGVYRLKHFGASKKTIVSPINYFTGASSAFAVQ 747
BLAST of Vigst.05G166500.01 vs. ExPASy TrEMBL (2024)
Match: tr|A0A2K3NNV7|A0A2K3NNV7_TRIPR (Neutral ceramidase OS=Trifolium pratense OX=57577 GN=L195_g001148 PE=3 SV=1) HSP 1 Score: 1233.78 bits (3191), Expect = 0.000e+0 Identity = 590/727 (81.16%), Postives = 640/727 (88.03%), Query Frame = 0
Query: 28 VQGTHGEYLIGVGSYDMTGPAADVNMMGYANMEQNTAGIHFRLRARTFIVGESPDGPRFVFVNLDACMASQLLTIKVLERLNSRFGKLYNEENVAISGTHTHGGPGGYMQYLVYSVTSLGFVKQSFDVIADAIEQSIIQAHNNLKPASIYLNIGEVKDGGINRSPSAYLQNPANERARYASNVDTQMTLLKFVDEESGESIGAFTWFATHGTSMTNKNKLITGDNKGVAARLFEDWFASQNTSSTTNSSVSDVGQLVKMAQSIKATRGKDCSKQASKASKVRKNDGSLFVAAFCQSNVGDVSPNVLGAFCIDTGKPCDFNHSSCNGNDQLCVGRGPGYPDEILSTKIIGERQFKSAVELFQSASEEVKGKIEYSHVYLNFTDIEVELESNKVVKTCPAALGPGFAAGTTDGPGAFGFQQGDLKINPFWKNLRDFLKEPSQYQVDCQNPKPVLLSTGEMFDPYAWAPAILPIQILRLGKLIILSVPGEFTTMAGRRLREAVKETLISSSNGEFNDETHVVIAGLTNTYSQYIATFEEYQHQRYEAASTLYGPHTLSAYIQEFKKLAQAMAEGKNITTKGPSPPDLSSVQISLLLGPFGDSPPAGIEFGDIKEDIAFPERGYFRKGDTPSATFWSSNPRYDLLTEGTFAAVERLQGERWIATYDDDDLSLFFKWKVDNSSMHGLATIEWEIPFGSVSGVYRLRHFGATRITITSPISYFTGASSAFAVQ 754
+Q THGEYLIGVGSYDMTGPAADVNMMGYAN EQNTAGIHFRLRARTFIV E+ GPRFVFVNLDA MASQLLTIK+LERL SRFG LY EENVAISGTHTH GPGGY+QY+VYSVTSLGFVKQSFD IA+A+EQSIIQA+NNLKP SI++N G+VK+ INRSPSAYL NPA ER+RY SNVDTQMT LKFVD SG+S G+F+WFATHGTSM+ NKLI+GDNK D+G+LVK A+SIKAT GKDC++Q S+ASKVRKNDGSLFV AFCQSNVGDVSPNVLGAFCID+GKPCDFNHSSCNGND LCVGRGPGYPDEILSTKIIGERQF+SAVELF SASEE+ GKI+Y HVYLNFTDIEVEL+SNKVVKTCPAALGP FAAGTTDGPG FGFQQGD +I+PFWK++RDFLK+PSQYQVDCQNPKPVLLS+GEMFDPY WAPAILPIQILRLGKLIILSVPGEFTTMAGRRLREAVKETLIS+S+GEFN+ETHVVIAGLTNTYSQYIATFEEY QRYEAASTLYGPHTLSAYIQEF KLAQAMA+G I KG SPPDL SVQISLLLGPFGD P G +FGDI EDIAFPE GYF KGD PSATFWS+NPRYDLLTEGTFA+VERLQGERWI T+DDDDLSL FKWKVDNSS HG ATIEWEIP ++SG+YRL+HFGA++ +I SPI YFTGASSAFAVQ
Sbjct: 1 MQSTHGEYLIGVGSYDMTGPAADVNMMGYANTEQNTAGIHFRLRARTFIVAENLQGPRFVFVNLDAGMASQLLTIKLLERLKSRFGNLYTEENVAISGTHTHAGPGGYLQYIVYSVTSLGFVKQSFDAIANAVEQSIIQANNNLKPGSIFINTGDVKEASINRSPSAYLLNPAEERSRYPSNVDTQMTFLKFVDGASGKSKGSFSWFATHGTSMSKNNKLISGDNK-------------------------DIGELVKKARSIKATGGKDCNQQTSQASKVRKNDGSLFVGAFCQSNVGDVSPNVLGAFCIDSGKPCDFNHSSCNGNDLLCVGRGPGYPDEILSTKIIGERQFRSAVELFGSASEELTGKIDYRHVYLNFTDIEVELDSNKVVKTCPAALGPAFAAGTTDGPGVFGFQQGDAEISPFWKSVRDFLKKPSQYQVDCQNPKPVLLSSGEMFDPYPWAPAILPIQILRLGKLIILSVPGEFTTMAGRRLREAVKETLISNSDGEFNNETHVVIAGLTNTYSQYIATFEEYYQQRYEAASTLYGPHTLSAYIQEFNKLAQAMAKGDKINEKGSSPPDLLSVQISLLLGPFGDPTPNGTKFGDINEDIAFPESGYFTKGDKPSATFWSANPRYDLLTEGTFASVERLQGERWIPTHDDDDLSLLFKWKVDNSSFHGFATIEWEIPKDAISGIYRLKHFGASKNSIASPIDYFTGASSAFAVQ 702
BLAST of Vigst.05G166500.01 vs. ExPASy Swiss-Prot (2024)
Match: sp|Q304B9|NCER2_ARATH (Neutral ceramidase 2 OS=Arabidopsis thaliana OX=3702 GN=NCER2 PE=3 SV=1) HSP 1 Score: 903.664 bits (2334), Expect = 0.000e+0 Identity = 438/718 (61.00%), Postives = 542/718 (75.49%), Query Frame = 0
Query: 35 YLIGVGSYDMTGPAADVNMMGYANMEQNTAGIHFRLRARTFIVGESPDGPRFVFVNLDACMASQLLTIKVLERLNSRFGKLYNEENVAISGTHTHGGPGGYMQYLVYSVTSLGFVKQSFDVIADAIEQSIIQAHNNLKPASIYLNIGEVKDGGINRSPSAYLQNPANERARYASNVDTQMTLLKFVDEESGESIGAFTWFATHGTSMTNKNKLITGDNKGVAARLFEDWF--------ASQNTSSTTNSSVSDVGQ----LVKMAQSIKATRGKDCSKQASKASKVRKNDGSLFVAAFCQSNVGDVSPNVLGAFCIDTGKPCDFNHSSCNGNDQLCVGRGPGYPDEILSTKIIGERQFKSAVELFQSASEEVKGKIEYSHVYLNFTDIEVELES----NKVVKTCPAALGPGFAAGTTDGPGAFGFQQGDLKINPFWKNLRDFLKEPSQYQVDCQNPKPVLLSTGEMFDPYAWAPAILPIQILRLGKLIILSVPGEFTTMAGRRLREAVKETLISSSNGEFNDETHVVIAGLTNTYSQYIATFEEYQHQRYEAASTLYGPHTLSAYIQEFKKLAQAMAEGKNITTKGPSPPDLSSVQISLLLGPFGDSPPAGIEFGDIKEDIAFPERGYFRKGDTPSATFWSSNPRYDLLTEGTFAAVERL-QGERWIATYDDDDLSLFFKW-KVDNSSMHGLATIEWEIPFGSVSGVYRLRHFGATR 734
YLIGVGSYD+TGPAADVNMMGYAN +Q +GIHFRLRAR FIV E P G R VFVNLDACMASQ++TIKVLERL +R+G+LY E+NVAISG HTH GPGGY+QY+ Y VTSLGFV+QSFDV+ + IEQSI+QAH +L+P S ++N G++ D G+NRSPS+YL NPA ER++Y +VD +MTL+KFVD + G + G+F WFATHGTSM+ N LI+GDNKG AAR EDWF +S+N ++ VSD + L+ +A + K++RG K ++VR FV+AFCQSN GDVSPN LG FCIDTG PCDFNHS+CNG ++LC GRGPGYPDE ST+IIGE+QFK AVELF A+E+++GKI Y H YL+F++++V + ++ VKTCPAA+G GFAAGTTDGPGAF F+QGD + N FW+ +R+ L+ P QV CQ PKP+LL TGEM +PY WAP+ILPIQILR+G+L+ILSVPGEFTTMAGRRLR+A+K LISS EF++ HVVIAGLTNTYSQYIATFEEY+ QRYE ASTLYG HTL+AYIQEFKKLA A+ G + +GP PPDL QISLL DS P G++FGD+K D+ P + FR+G +ATFWS PR DL+TEG+FA VE L +G +W YDDDD SL FKW + S ATIEW +P +V+GVYR+RH+GA++
Sbjct: 26 YLIGVGSYDITGPAADVNMMGYANSDQIASGIHFRLRARAFIVAE-PQGNRVVFVNLDACMASQIVTIKVLERLKARYGELYTEKNVAISGIHTHAGPGGYLQYVTYIVTSLGFVRQSFDVVVNGIEQSIVQAHESLRPGSAFVNKGDLLDAGVNRSPSSYLNNPAAERSKYKYDVDKEMTLVKFVDSQLGPT-GSFNWFATHGTSMSRTNSLISGDNKGAAARFMEDWFENGQKNSVSSRNIPRRVSTIVSDFSRNRDRLLDIAATYKSSRGHSVDKSLDVKTRVRNGSKRKFVSAFCQSNCGDVSPNTLGTFCIDTGLPCDFNHSTCNGQNELCYGRGPGYPDEFESTRIIGEKQFKMAVELFNKATEKLQGKIGYQHAYLDFSNLDVTVPKAGGGSETVKTCPAAMGFGFAAGTTDGPGAFDFKQGDDQGNVFWRLVRNVLRTPGPEQVQCQKPKPILLDTGEMKEPYDWAPSILPIQILRIGQLVILSVPGEFTTMAGRRLRDAIKSFLISSDPKEFSNNMHVVIAGLTNTYSQYIATFEEYEVQRYEGASTLYGRHTLTAYIQEFKKLATALVNGLTL-PRGPQPPDLLDKQISLLSPVVVDSTPLGVKFGDVKADV--PPKSTFRRGQQVNATFWSGCPRNDLMTEGSFAVVETLREGGKWAPVYDDDDFSLKFKWSRPAKLSSESQATIEWRVPESAVAGVYRIRHYGASK 738
BLAST of Vigst.05G166500.01 vs. ExPASy Swiss-Prot (2024)
Match: sp|Q0JL46|NCASE_ORYSJ (Neutral ceramidase OS=Oryza sativa subsp. japonica OX=39947 GN=Os01g0624000 PE=1 SV=1) HSP 1 Score: 883.634 bits (2282), Expect = 0.000e+0 Identity = 442/752 (58.78%), Postives = 555/752 (73.80%), Query Frame = 0
Query: 35 YLIGVGSYDMTGPAADVNMMGYANMEQNTAGIHFRLRARTFIVGESPDGPRFVFVNLDACMASQLLTIKVLERLNSRFGKLYNEENVAISGTHTHGGPGGYMQYLVYSVTSLGFVKQSFDVIADAIEQSIIQAHNNLKPASIYLNIGEVKDGGINRSPSAYLQNPANERARYASNVDTQMTLLKFVDEESGESIGAFTWFATHGTSMTNKNKLITGDNKGVAARLFEDW-----------------FASQNTSSTTNSSVSDV--------GQLVKMAQSIKATRGKDCSKQA-SKASKVRKNDGSLFVAAFCQSNVGDVSPNVLGAFCIDTGKPCDFNHSSCNGNDQLCVGRGPGYPDEILSTKIIGERQFKSAVELFQSASEEVKGKIEYSHVYLNFTDIEVELESN----KVVKTCPAALGPGFAAGTTDGPGAFGFQQGDLKINPFWKNLRDFLKEPSQYQVDCQNPKPVLLSTGEMFDPYAWAPAILPIQILRLGKLIILSVPGEFTTMAGRRLREAVKETLISSSNGEFNDETHVVIAGLTNTYSQYIATFEEYQHQRYEAASTLYGPHTLSAYIQEFKKLAQAMAEGKNITTKGPSPPDLSSVQISLLLGPFGDSPPAGIEFGDIKEDIAFPERGYFRKGDTPSATFWSSNPRYDLLTEGTFAAVERLQG-ERWIATYDDDDLSLFFKW-KVDNSSMHGLATIEWEIPFGSVSGVYRLRHFGATRITITSPISYFTGASSAFAVQ 754
YL+G+GS+D+TGPAADVNMMGYAN EQ +GIHFRL++R FIV E P+G R VFVN+DACMASQ++TIKVLERL +R+G LYNE NVAISG HTH GPGGY+QY+VY VTSLGFV+QSFDVI D IEQSI++AHNNL+P I++N G++ D G+NRSPSAYL NPA ER++Y NVD +MTL+KFVD+E G +G+F WFATHGTSM+ N LI+GDNKG AAR EDW S + +S VS + L+++A S +A+ G+ + + ++ + + + FV+AFCQSN GDVSPNVLG FCIDT PCDFNHS+CNG ++LC GRGPGYPDE ST++IG RQF A +LF SASEE++GKI+Y H YL+F+ +EV++ ++ + VKTCPAA+G FAAGTTDGPGAF F+QGD+K NPFWK +R+ LK P + QV+C +PKP+LL TGEM +PY WAPAILP+Q++R+G+L+IL VPGEFTTMAGRRLR+AVK T+++S N EF+ HVV+AGLTN+YSQYI TFEEYQ QRYE ASTLYGPHTLSAYIQEF+KLA AM K + T PPD+ QI LL G DS P G++FGD+ D+ P F KG T +ATF+S+ PR DLLT+GTFA VE+L G W+ YDDDD SL FKW + S AT+EW +P + +GVYRLRHFGA++ S + +FTG S AFAV+
Sbjct: 41 YLVGMGSFDITGPAADVNMMGYANTEQIASGIHFRLKSRAFIVAE-PNGKRVVFVNIDACMASQIVTIKVLERLKARYGDLYNENNVAISGIHTHAGPGGYLQYVVYIVTSLGFVRQSFDVIVDGIEQSIVEAHNNLRPGKIFVNKGDLLDAGVNRSPSAYLNNPAEERSKYEYNVDKEMTLIKFVDDELG-PVGSFNWFATHGTSMSRTNSLISGDNKGAAARFMEDWAEQMGLPKQSAHANSDDLRSLHKTSVLPRRVSTIIPEPNEITDDLIQLASSYEASGGRRLAGSSITRRIRSTQQNKPKFVSAFCQSNCGDVSPNVLGTFCIDTNLPCDFNHSTCNGKNELCYGRGPGYPDEFESTRVIGNRQFLKARDLFDSASEEIQGKIDYRHTYLDFSKLEVKVSTSAGGQQTVKTCPAAMGFAFAAGTTDGPGAFDFRQGDVKGNPFWKLVRNLLKTPGKDQVECHSPKPILLDTGEMKEPYDWAPAILPVQMIRIGQLVILCVPGEFTTMAGRRLRDAVK-TVLTSGNSEFDKNIHVVLAGLTNSYSQYITTFEEYQIQRYEGASTLYGPHTLSAYIQEFQKLAMAMIANKEVPTNF-QPPDMLDKQIGLLPGVVFDSTPLGVKFGDVNSDV--PGNSTFNKGSTVNATFYSACPRNDLLTDGTFALVEKLDGNNNWVPVYDDDDWSLRFKWSRPARLSSRSFATLEWTVPEDAAAGVYRLRHFGASKPMFGS-VRHFTGTSRAFAVR 785
BLAST of Vigst.05G166500.01 vs. ExPASy Swiss-Prot (2024)
Match: sp|F4HQM3|NCER1_ARATH (Neutral ceramidase 1 OS=Arabidopsis thaliana OX=3702 GN=NCER1 PE=2 SV=1) HSP 1 Score: 859.366 bits (2219), Expect = 0.000e+0 Identity = 437/753 (58.03%), Postives = 544/753 (72.24%), Query Frame = 0
Query: 31 THGEYLIGVGSYDMTGPAADVNMMGYANMEQNTAGIHFRLRARTFIVGESPDGPRFVFVNLDACMASQLLTIKVLERLNSRFGKLYNEENVAISGTHTHGGPGGYMQYLVYSVTSLGFVKQSFDVIADAIEQSIIQAHNNLKPASIYLNIGEVKDGGINRSPSAYLQNPANERARYASNVDTQMTLLKFVDEESGESIGAFTWFATHGTSMTNKNKLITGDNKGVAARLFEDWFASQNTSSTTNSS--VSDV----------------GQLVKMAQSIKATRGKDCSKQASKASKVR----KNDGSLFVAAFCQSNVGDVSPNVLGAFCIDTGKPCDFNHSSCNGNDQLCVGRGPGYPDEILSTKIIGERQFKSAVELFQSASEEVKGKIEYSHVYLNFTDIEVELES----NKVVKTCPAALGPGFAAGTTDGPGAFGFQQGDLKINPFWKNLRDFLKEPSQYQVDCQNPKPVLLSTGEMFDPYAWAPAILPIQILRLGKLIILSVPGEFTTMAGRRLREAVKETLISSSNGEFNDETHVVIAGLTNTYSQYIATFEEYQHQRYEAASTLYGPHTLSAYIQEFKKLAQAMAEGKNITTKGPSPPDLSSVQISLLLGPFGDSPPAGIEFGDIKEDIAFPERGYFRKGD-TPSATFWSSNPRYDLLTEGTFAAVERLQ--GERWIATYDDDDLSLFFKW-KVDNSSMHGLATIEWEIPFGSVSGVYRLRHFGATRITITSPISYFTGASSAFAV 753
+H EYLIG+GSYD+TGPAADVNMMGYANMEQ +GIHFRLRARTFIV E P G R VFVNLDACMASQ++ +KV+ERL +R+G LY E+NV ISG HTH GPGGY+QY+VY VTSLGFV+QSFD + D IE SIIQAH NL+P SI+LN GE+ D G+NRSPSAYL NP+ ER+++ NVD +MTLLKFVD++ G +G+F WFATHGTSM+ N LI+GDNKG A+R EDW+ QNT+ + S +SD +L+++A ++ GK ++ +S A +VR K D FV+AFCQ+N GDVSPNVLGAFC+DTG PCDFNHS+C G +++C GRGPGYPDE ST+IIGERQFK A+ELF ASE+++GK++Y HVY++F+ + V L ++VVKTCPAA+G FAAGTTDGPGAF F QGD K NPFW+ +R+ LK P + Q+DC PKP+LL TGEM PY WAP+IL +Q+LR+G+L ILSVPGEFTTMAGRRLR AVK L +S N + + E HVVIAGL N YSQY+ TFEEYQ QRYE ASTLYGPHTLS YIQEFKKL++++ + GP PPDL Q+S L D+ P+G FGD+ D+ P+ ++G+ + F S+ PR DLLTEGTF VERL+ + W YDDDDL L FKW + S AT+EW IP + GVYR+ HFGA + S + +FTG+SSAF V
Sbjct: 33 SHSEYLIGLGSYDITGPAADVNMMGYANMEQVASGIHFRLRARTFIVSE-PQGKRVVFVNLDACMASQIVKLKVIERLKARYGDLYTEQNVGISGIHTHAGPGGYLQYVVYIVTSLGFVRQSFDALVDGIENSIIQAHENLRPGSIFLNNGELLDAGVNRSPSAYLNNPSKERSKHKYNVDKEMTLLKFVDDQWG-PVGSFNWFATHGTSMSRTNSLISGDNKGAASRFMEDWY-EQNTAERSYSEEFISDEIPRRVSSLIENHQDSHHELLELASYFESQPGKPVTRISSSARRVRSALRKADKPGFVSAFCQTNCGDVSPNVLGAFCLDTGLPCDFNHSTCGGKNEMCYGRGPGYPDEFESTRIIGERQFKMALELFNKASEQLQGKVDYRHVYVDFSQLNVTLPKKDGKSEVVKTCPAAMGFAFAAGTTDGPGAFDFTQGDDKGNPFWRLVRNVLKTPDKKQIDCHYPKPILLDTGEMTKPYDWAPSILSLQVLRIGQLFILSVPGEFTTMAGRRLRYAVKTQLKNSGNKDLSGEIHVVIAGLANGYSQYVTTFEEYQVQRYEGASTLYGPHTLSGYIQEFKKLSKSLVLDMPV-QPGPQPPDLLDKQLSFLTPVMMDTTPSGDSFGDVISDV--PKNLSLKRGNGQVTVVFRSACPRNDLLTEGTFTLVERLEQKDKTWTPVYDDDDLCLRFKWSRHKKLSSRSQATVEWRIPESASPGVYRITHFGAAKKLFGS-VHHFTGSSSAFVV 778
BLAST of Vigst.05G166500.01 vs. ExPASy Swiss-Prot (2024)
Match: sp|F4KHQ8|NCER3_ARATH (Neutral ceramidase 3 OS=Arabidopsis thaliana OX=3702 GN=NCER3 PE=2 SV=1) HSP 1 Score: 835.484 bits (2157), Expect = 0.000e+0 Identity = 430/742 (57.95%), Postives = 522/742 (70.35%), Query Frame = 0
Query: 34 EYLIGVGSYDMTGPAADVNMMGYANMEQNTAGIHFRLRARTFIVGESPDGPRFVFVNLDACMASQLLTIKVLERLNSRFGKLYNEENVAISGTHTHGGPGGYMQYLVYSVTSLGFVKQSFDVIADAIEQSIIQAHNNLKPASIYLNIGEVKDGGINRSPSAYLQNPANERARYASNVDTQMTLLKFVDEESGESIGAFTWFATHGTSMTNKNKLITGDNKGVAARLFEDWFASQNTSSTTN--------SSVSDV--GQLVKMAQSIKATRGKDCSKQASKASKVRKN----DGSLFVAAFCQSNVGDVSPNVLGAFCIDTGKPCDFNHSSCNGNDQLCVGRGPGYPDEILSTKIIGERQFKSAVELFQSASEEVKGKIEYSHVYLNFTDIEVELE----SNKVVKTCPAALGPGFAAGTTDGPGAFGFQQGDLKINPFWKNLRDFLKEPSQYQVDCQNPKPVLLSTGEMFDPYAWAPAILPIQILRLGKLIILSVPGEFTTMAGRRLREAVKETLISSSNGEFNDETHVVIAGLTNTYSQYIATFEEYQHQRYEAASTLYGPHTLSAYIQEFKKLAQAMAEGKNITTKGPSPPDLSSVQISLLLGPFGDSPPAGIEFGDIKEDIAFPERGYFRKG-DTPSATFWSSNPRYDLLTEGTFAAVER-LQG-ERWIATYDDDDLSLFFKW-KVDNSSMHGLATIEWEIPFGSVSGVYRLRHFGATRITITSPISYFTGASSAFAV 753
+YL+G+GSYD+TGPAADVNMMGYANMEQ +G+HFRLRAR FIV E P R FVNLDA MASQL+TIKV+ERL R+G+LY EENVAISGTHTH GPGGY+QY++Y VTSLGFV QSF+ + D IEQSIIQAH NL+P SI +N GE+ D G+NRSPSAYL NPA+ER++Y +VD +MTL+KFVD++ W G AR+ EDWF +N + + S +SD L++MA S+ +T GK ++ +S A +VR D FV+AFCQ+N GDVSPNVLGAFCIDTG PC+FN S+C G ++ C GRGPGYPDE ST+IIGERQFK A +LF ASEE++GK++Y H Y++F+ +EV + ++VVKTCPAA+G GFAAGTTDGPGAF F+QGD + NPFW+ +R+ LK P++ QV CQ PKP+LL TGEM PY WAP+ILP+QILR+G+L+IL VPGEFTTMAGRRLR+AVK L SNG E VVIAGLTN+YSQYIATFEEYQ QRYE ASTLYGPHTLS YIQEFKKLA + + T GP PPDL QISLL D P G FGD+ D+ P FRKG D F S+NPR DL+TEGTFA VER L+G E W+ YDDDD L FKW + S ATIEW IP + GVYR+ HFG+ + I+S I +F+G+SSAF V
Sbjct: 28 DYLMGLGSYDITGPAADVNMMGYANMEQVASGVHFRLRARAFIVAE-PYKKRIAFVNLDAGMASQLVTIKVIERLKQRYGELYTEENVAISGTHTHAGPGGYLQYILYLVTSLGFVHQSFNALVDGIEQSIIQAHENLRPGSILINKGELLDAGVNRSPSAYLNNPAHERSKYEYDVDKEMTLVKFVDDQ---------W--------------------GPVARIMEDWFERENGCRSVDVESPRRVSSIISDPYDQDLMEMASSLLSTGGKTVTRMSSVARRVRSRFRHADKPRFVSAFCQTNCGDVSPNVLGAFCIDTGLPCEFNQSTCGGKNEQCYGRGPGYPDEFESTRIIGERQFKKAADLFTKASEEIQGKVDYRHAYVDFSQLEVTINGQNGGSEVVKTCPAAMGFGFAAGTTDGPGAFDFKQGDDQGNPFWRLVRNLLKNPTEEQVRCQRPKPILLDTGEMKQPYDWAPSILPVQILRIGQLVILCVPGEFTTMAGRRLRDAVKTVLKEGSNGR---EFSVVIAGLTNSYSQYIATFEEYQVQRYEGASTLYGPHTLSGYIQEFKKLANDLLSAQT-TDPGPQPPDLLHKQISLLTPVVADMTPIGTAFGDVTSDV--PRLSKFRKGADIVRVQFRSANPRNDLMTEGTFALVERWLEGRETWVPVYDDDDFCLRFKWSRPFKLSTQSTATIEWRIPETASPGVYRITHFGSAKTPISS-IHHFSGSSSAFVV 732
BLAST of Vigst.05G166500.01 vs. ExPASy Swiss-Prot (2024)
Match: sp|Q55G11|NCSEB_DICDI (Neutral ceramidase B OS=Dictyostelium discoideum OX=44689 GN=dcd2B PE=3 SV=1) HSP 1 Score: 571.237 bits (1471), Expect = 0.000e+0 Identity = 318/729 (43.62%), Postives = 439/729 (60.22%), Query Frame = 0
Query: 35 YLIGVGSYDMTGPAADVNMMGYANMEQNTAGIHFRLRARTFIVGESPDGPRFVFVNLDACMASQLLTIKVLERLNSRFG-KLYNEENVAISGTHTHGGPGGYMQYLVYSVTSLGFVKQSFDVIADAIEQSIIQAHNNLKPASIYLNIGEVKDGGINRSPSAYLQNPANERARYASNVDTQMTLLKFVDEESGESIGAFTWFATHGTSMTNKNKLITGDNKGVAARLFEDWFASQNTSSTTNSSVSDVGQLVKMAQSIKATRGKDCSKQASKASKVRKNDGSLFVAAFCQSNVGDVSPNVLGAFCIDTGKPCDFNHSSCNGNDQLCVGRGPGYPDEIL-STKIIGERQFKSAVELFQSASEEVKGKIEYSHVYLNFTDIEVELESNKVVK---TCPAALGPGFAAGTTDGPGAFGFQQGDLKI--NPFWKNLRDFLKEPSQYQVDCQNPKPVLLSTGEMFDPYAWAPAILPIQILRLGKLIILSVPGEFTTMAGRRLREAVKETLISSSNGEFNDETHVVIAGLTNTYSQYIATFEEYQHQRYEAASTLYGPHTLSAYIQEFKKLAQAMAEGKNITTKGPSPPDLSSVQISLLLGPFGDSPPAGIEFGDIKEDIAFPERGYFRKGDTPSATFWSSNPRYDLLTEGTFAAVERLQG-ERWIATYDDDDLSLFFKWKVDNSSMHGLATIEWEIPFGSVSGVYRLRHFG-ATRITITSPISYFTGASSAFAVQ 754
Y IG G YD+TG +A+VN+MGYAN Q AGIHFR RAR F+ +S +G R V+V+ D+CM Q + I V+E L FG +Y E NV +SGTHTH GP G+ QY +Y +TSLGF K++FD I + I Q+I++AH +++PA+++ GE+ + INRSP AY NP E+A Y SNVD MT+L+ +++ +G A ++FA H TSM N N LI+GDNKG A+ L+E KQ + K FVAAF QSN GDVSPN G C D G PCD+ S+CNG ++ C GPG ++ ST+IIG QF A+ELF +AS +V G ++Y H ++ FT++ VE N V TC A+G FAAGTTDGPGAF F Q D NPFW + DF+ +P+ Q+ CQ+PKP+LL G M +P W P ++PIQI+ +G++++++VPGEFTTM+GRRLR +V+E + GE + V+IAGL+NTYS YIATFEEYQ QRYE AST++GPHTL +Y+QEF KLAQ++ +G + GP+P +L+ + L D+ P +FG++ D+ + +T S F+ NPR D + E +F +V+ L G ++W DD D FKWK+ + L TIEW I + G YR+ H G A + +S ++ + G S F VQ
Sbjct: 46 YQIGAGIYDITGASAEVNLMGYANPLQVGAGIHFRQRARAFVFVDS-NGNRAVYVSTDSCMIFQEVKIHVVELLQDIFGPNVYTEANVLLSGTHTHSGPAGFSQYALYGITSLGFYKKNFDTICNGIVQAIVKAHKSVQPANMFTETGELWNTNINRSPFAYDNNPEEEKAMYDSNVDKNMTVLR-IEDMNGNPFAAISFFAVHCTSMNNTNHLISGDNKGYASYLWE--------------------------------------KQVNGPGTAGKGP---FVAAFGQSNEGDVSPNTRGPTCRD-GSPCDYKTSTCNGRNEECWSLGPGKDGDMFESTQIIGGNQFNKALELFNNASIQVSGPVQYRHSWVQFTNVSVEPPYNSGVDNATTCRGAMGYSFAAGTTDGPGAFNFVQSDNNTSGNPFWNFIGDFIAKPTPDQIRCQSPKPILLDVG-MVEPIPWVPDVMPIQIVTIGQIVLVAVPGEFTTMSGRRLRNSVREII-----GESIENPIVLIAGLSNTYSGYIATFEEYQVQRYEGASTVFGPHTLGSYMQEFGKLAQSIVDGTTVPA-GPTPRNLTGHTLFFLPPVIVDAAPDFDDFGEVSIDVNL----NYSVNETVSCVFYGGNPRNDFMIESSFLSVDLLTGTDQWTTVLDDGDWDTKFKWKMHDLGF-SLITIEWVIAPDTTPGTYRITHSGFAKKNPFSSNLTPYQGISRNFVVQ 718
BLAST of Vigst.05G166500.01 vs. ExPASy Swiss-Prot (2024)
Match: sp|Q9NR71|ASAH2_HUMAN (Neutral ceramidase OS=Homo sapiens OX=9606 GN=ASAH2 PE=1 SV=2) HSP 1 Score: 550.051 bits (1416), Expect = 0.000e+0 Identity = 319/737 (43.28%), Postives = 418/737 (56.72%), Query Frame = 0
Query: 35 YLIGVGSYDMTGPAADVNMMGYANMEQNTAGIHFRLRARTFIVGESPDGP-RFVFVNLDACMASQLLTIKVLERLNSRFGKLYNEENVAISGTHTHGGPGGYMQYLVYSVTSLGFVKQSFDVIADAIEQSIIQAHNNLKPASIYLNIGEVKDGGINRSPSAYLQNPANERARYASNVDTQMTLLKFVDEESGESIGAFTWFATHGTSMTNKNKLITGDNKGVAARLFEDWFASQNTSSTTNSSVSDVGQLVKMAQSIKATRGKDCSKQASKASKVRKNDGSL-----FVAAFCQSNVGDVSPNVLGAFCIDTGKPCDFNHSSCN-GNDQLCVGRGPGYPDEILSTKIIGERQFKSAVELFQSASEEVKGKIEYSHVYLNFTDIEVELESNKVVKTCPAALGPGFAAGTTDGPGAFGFQQGDLKINPFWKNLRD-FLKEPSQYQVDCQNPKPVLLSTGEMFDPYAWAPAILPIQILRLGKLIILSVPGEFTTMAGRRLREAVKETLISSSNGEFNDETHVVIAGLTNTYSQYIATFEEYQHQRYEAASTLYGPHTLSAYIQEFKKLAQAMAEGKNIT-TKGPSPPDLSSVQISLLLGPFGDSPPAGIEFGDIKEDIAFPERGYFRKGDTPSATFWSSNPRYDL--LTEGTFAAVERLQG--ERWIATYDDDDLSLFFKWKVDNSSMHGL--ATIEWEIPFGSVSGVYRLRHFGATR---ITITSPISYFTGASSAFAV 753
Y IGVG D TG AD+N+MGY QN GI RL +R FI+ E PDG R VFV++D M SQ L ++VL RL S++G LY +NV +SGTHTH GP GY QY V+ + S GF Q+F + I +SI AH N+KP I++N G V INRSP +YLQNP +ERARY+SN D +M +LK VD +G+ +G +WFA H SM N N L+ DN G A+ L E KN G L FVAAF SN+GDVSPN+LG CI+TG+ CD +S+C G +C+ +GPG D ST+IIG ++ A EL+ SAS+EV G + +H +++ TD+ V L S KTC ALG FAAGT DG G F QG + +PFW +RD L +PS+ +C PKP+LL TGE+ P+ W P I+ +QI+ LG L I ++PGEFTTM+GRRLREAV+ +S+G N VVI+GL N Y+ YI T+EEYQ QRYEAAST+YGPHTLSAYIQ F+ LA+A+A ++GP PP + I L+ D P G FGD+ + P + +R G+ F +NP+ + T TF VE+ + W +D F W + + GL AT+EW IP + G+YR+R+FG R I + I F G S AF V
Sbjct: 102 YHIGVGRADCTGQVADINLMGYGKSGQNAQGILTRLYSRAFIMAE-PDGSNRTVFVSIDIGMVSQRLRLEVLNRLQSKYGSLYRRDNVILSGTHTHSGPAGYFQYTVFVIASEGFSNQTFQHMVTGILKSIDIAHTNMKPGKIFINKGNVDGVQINRSPYSYLQNPQSERARYSSNTDKEMIVLKMVDL-NGDDLGLISWFAIHPVSMNNSNHLVNSDNVGYASYLLEQ----------------------------------------------EKNKGYLPGQGPFVAAFASSNLGDVSPNILGPRCINTGESCDNANSTCPIGGPSMCIAKGPGQ-DMFDSTQIIGRAMYQRAKELYASASQEVTGPLASAHQWVDMTDVTVWLNSTHASKTCKPALGYSFAAGTIDGVGGLNFTQGKTEGDPFWDTIRDQILGKPSEEIKECHKPKPILLHTGELSKPHPWHPDIVDVQIITLGSLAITAIPGEFTTMSGRRLREAVQAEF--ASHGMQN--MTVVISGLCNVYTHYITTYEEYQAQRYEAASTIYGPHTLSAYIQLFRNLAKAIATDTVANLSRGPEPPFFKQL-IVPLIPSIVDRAPKGRTFGDVLQ----PAKPEYRVGEVAEVIFVGANPKNSVQNQTHQTFLTVEKYEATSTSWQIVCNDASWETRFYW---HKGLLGLSNATVEWHIPDTAQPGIYRIRYFGHNRKQDILKPAVILSFEGTSPAFEV 777
BLAST of Vigst.05G166500.01 vs. ExPASy Swiss-Prot (2024)
Match: sp|Q91XT9|ASAH2_RAT (Neutral ceramidase OS=Rattus norvegicus OX=10116 GN=Asah2 PE=1 SV=1) HSP 1 Score: 544.273 bits (1401), Expect = 0.000e+0 Identity = 317/738 (42.95%), Postives = 423/738 (57.32%), Query Frame = 0
Query: 35 YLIGVGSYDMTGPAADVNMMGYANMEQNTAGIHFRLRARTFIVGESPDGP-RFVFVNLDACMASQLLTIKVLERLNSRFGKLYNEENVAISGTHTHGGPGGYMQYLVYSVTSLGFVKQSFDVIADAIEQSIIQAHNNLKPASIYLNIGEVKDGGINRSPSAYLQNPANERARYASNVDTQMTLLKFVDEESGESIGAFTWFATHGTSMTNKNKLITGDNKGVAARLFEDWFASQNTSSTTNSSVSDVGQLVKMAQSIKATRGKDCSKQASKASKVRKNDGSL-----FVAAFCQSNVGDVSPNVLGAFCIDTGKPCDFNHSSC-NGNDQLCVGRGPGYPDEILSTKIIGERQFKSAVELFQSASEEVKGKIEYSHVYLNFTDIEVELESNKVVKTCPAALGPGFAAGTTDGPGAFGFQQGDLKINPFWKNLRD-FLKEPSQYQVDCQNPKPVLLSTGEMFDPYAWAPAILPIQILRLGKLIILSVPGEFTTMAGRRLREAVKETLISSSNGEFNDETHVVIAGLTNTYSQYIATFEEYQHQRYEAASTLYGPHTLSAYIQEFKKLAQAMAEGK--NITTKGPSPPDLSSVQISLLLGPFGDSPPAGIEFGDIKEDIAFPERGYFRKGDTPSATFWSSNPR--YDLLTEGTFAAVERLQGE--RWIATYDDDDLSLFFKWKVDNSSMHGL--ATIEWEIPFGSVSGVYRLRHFGATR---ITITSPISYFTGASSAFAV 753
Y IGVG D TG +D+N+MGY QN G+ RL +R FI+ + PDG R FV+++ CM SQ L ++VL+RL S++G LY +NV +S THTH GP G+ QY +Y + S GF ++F I I +SI AH NLKP + +N G V + INRSPS+YLQNP +ERARY+S+ D +M +LK VD +GE +G +WFA H SM N N L+ DN G AA LFE KN G L FVA F SN+GDVSPN+LG C++TG+ CD + S+C +G +C+ GPG D ST IIG ++ A EL SAS+EV G + +H ++N TD+ V+L + VKTC AALG FAAGT DG QG + N FW LRD L +PS+ ++CQ PKP+L+ TGE+ P+ W P I+ IQI+ LG L I ++PGEFTTM+GRRLREAVK+ D T VVIAGL+N Y+ YI T+EEYQ QRYEAAST+YGPHTLSAYIQ F+ LA+A+A N+++ GP PP ++ I L+ D P G +FGD+ + P + +R G+ F +NP+ + T TF VE+ + W ++D F W + + GL ATI W IP ++ GVYR+R+FG R + + I F G SS F +
Sbjct: 83 YYIGVGRADCTGQVSDINLMGYGKNGQNAQGLLTRLFSRAFILAD-PDGSNRMAFVSVELCMISQRLRLEVLKRLQSKYGSLYRRDNVILSATHTHSGPAGFFQYTLYILASEGFSNRTFQYIVSGIVKSIDIAHTNLKPGKVLINKGNVANVQINRSPSSYLQNPPSERARYSSDTDKEMVVLKLVDL-NGEDLGLISWFAVHPVSMNNSNHLVNSDNMGYAAYLFEQ----------------------------------------------EKNRGYLPGQGPFVAGFASSNLGDVSPNILGPHCVNTGESCDNDKSTCPSGGPSMCMASGPGQ-DMFESTHIIGRVIYQKAKELHASASQEVTGPVLTAHQWVNMTDVSVQLNATHTVKTCKAALGYSFAAGTIDGVSGLNITQGTTEGNLFWDTLRDQLLGKPSEEIIECQKPKPILIHTGELTKPHPWQPDIVDIQIVTLGSLAIAAIPGEFTTMSGRRLREAVKKEFALYG---MKDMT-VVIAGLSNVYTHYITTYEEYQAQRYEAASTIYGPHTLSAYIQLFRALAKAIATDTVANMSS-GPEPPFFKNL-IGSLIPNIADRAPIGKQFGDVLQ----PAKPEYRVGEVVEVVFVGANPKNSAENQTHQTFLTVEKYEDSVANWQIMHNDASWETRFYW---HKGVLGLSNATIHWHIPDTALPGVYRIRYFGHNRKQELLKPAVILAFEGISSPFEI 758
BLAST of Vigst.05G166500.01 vs. ExPASy Swiss-Prot (2024)
Match: sp|Q9JHE3|ASAH2_MOUSE (Neutral ceramidase OS=Mus musculus OX=10090 GN=Asah2 PE=1 SV=1) HSP 1 Score: 543.117 bits (1398), Expect = 0.000e+0 Identity = 313/738 (42.41%), Postives = 419/738 (56.78%), Query Frame = 0
Query: 35 YLIGVGSYDMTGPAADVNMMGYANMEQNTAGIHFRLRARTFIVGESPDGP-RFVFVNLDACMASQLLTIKVLERLNSRFGKLYNEENVAISGTHTHGGPGGYMQYLVYSVTSLGFVKQSFDVIADAIEQSIIQAHNNLKPASIYLNIGEVKDGGINRSPSAYLQNPANERARYASNVDTQMTLLKFVDEESGESIGAFTWFATHGTSMTNKNKLITGDNKGVAARLFEDWFASQNTSSTTNSSVSDVGQLVKMAQSIKATRGKDCSKQASKASKVRKNDGSL-----FVAAFCQSNVGDVSPNVLGAFCIDTGKPCDFNHSSC-NGNDQLCVGRGPGYPDEILSTKIIGERQFKSAVELFQSASEEVKGKIEYSHVYLNFTDIEVELESNKVVKTCPAALGPGFAAGTTDGPGAFGFQQGDLKINPFWKNLRD-FLKEPSQYQVDCQNPKPVLLSTGEMFDPYAWAPAILPIQILRLGKLIILSVPGEFTTMAGRRLREAVKETLISSSNGEFNDETHVVIAGLTNTYSQYIATFEEYQHQRYEAASTLYGPHTLSAYIQEFKKLAQAMAEGK--NITTKGPSPPDLSSVQISLLLGPFGDSPPAGIEFGDIKEDIAFPERGYFRKGDTPSATFWSSNPR--YDLLTEGTFAAVERLQGE--RWIATYDDDDLSLFFKWKVDNSSMHGL--ATIEWEIPFGSVSGVYRLRHFGATR---ITITSPISYFTGASSAFAV 753
Y IGVG D TG +D+N+MGY QN G+ RL +R FI+ + PDG R FV+++ CM SQ L ++VL+RL S++G LY +NV +S HTH GP G+ QY +Y + S GF ++F I I +SI AH NLKP I++N G V + INRSPS+YL NP +ERARY+SN D +M +LK VD +GE +G +WFA H SM N N + DN G AA LFE KN G L FVA F SN+GDVSPN+LG C++TG+ CD + S+C NG +C+ GPG D ST IIG ++ A EL+ SAS+EV G + +H ++N TD+ V+L + VKTC ALG FAAGT DG QG + +PFW LRD L +PS+ V+CQ PKP+LL +GE+ P+ W P I+ +QI+ +G L I ++PGE TTM+GRR REA+K+ D T VVIAGL+N Y+ YI T+EEYQ QRYEAAST+YGPHTLSAYIQ F+ LA+A+A N+++ GP PP ++ I+ L+ D P G FGD+ + P + +R G+ F +NP+ + T TF VE+ + W Y+D F W + + GL ATI W IP + G+YR+R+FG R + + I F G SS F V
Sbjct: 78 YYIGVGRADCTGQVSDINLMGYGKNGQNARGLLTRLFSRAFILAD-PDGSNRMAFVSVELCMISQRLRLEVLKRLESKYGSLYRRDNVILSAIHTHSGPAGFFQYTLYILASEGFSNRTFQYIVSGIMKSIDIAHTNLKPGKIFINKGNVANVQINRSPSSYLLNPQSERARYSSNTDKEMLVLKLVDL-NGEDLGLISWFAIHPVSMNNSNHFVNSDNMGYAAYLFEQ----------------------------------------------EKNKGYLPGQGPFVAGFASSNLGDVSPNILGPHCVNTGESCDNDKSTCPNGGPSMCMASGPGQ-DMFESTHIIGRIIYQKAKELYASASQEVTGPVLAAHQWVNMTDVSVQLNATHTVKTCKPALGYSFAAGTIDGVSGLNITQGTTEGDPFWDTLRDQLLGKPSEEIVECQKPKPILLHSGELTIPHPWQPDIVDVQIVTVGSLAIAAIPGELTTMSGRRFREAIKKEFALYG---MKDMT-VVIAGLSNVYTHYITTYEEYQAQRYEAASTIYGPHTLSAYIQLFRDLAKAIATDTVANMSS-GPEPPFFKNL-IASLIPNIADRAPIGKHFGDVLQ----PAKPEYRVGEVVEVIFVGANPKNSAENQTHQTFLTVEKYEDSVADWQIMYNDASWETRFYW---HKGILGLSNATIYWHIPDTAYPGIYRIRYFGHNRKQELLKPAVILAFEGISSPFEV 753
BLAST of Vigst.05G166500.01 vs. ExPASy Swiss-Prot (2024)
Match: sp|Q5W7F1|ASAH2_DANRE (Neutral ceramidase OS=Danio rerio OX=7955 GN=asah2 PE=1 SV=1) HSP 1 Score: 542.347 bits (1396), Expect = 0.000e+0 Identity = 302/728 (41.48%), Postives = 412/728 (56.59%), Query Frame = 0
Query: 35 YLIGVGSYDMTGPAADVNMMGYANMEQNTAGIHFRLRARTFIVGESPDGPRFVFVNLDACMASQLLTIKVLERLNSRFGKLYNEENVAISGTHTHGGPGGYMQYLVYSVTSLGFVKQSFDVIADAIEQSIIQAHNNLKPASIYLNIGEVKDGGINRSPSAYLQNPANERARYASNVDTQMTLLKFVDEESGESIGAFTWFATHGTSMTNKNKLITGDNKGVAARLFEDWFASQNTSSTTNSSVSDVGQLVKMAQSIKATRGKDCSKQASKASKVRKNDGSLFVAAFCQSNVGDVSPNVLGAFCIDTGKPCDFNHSSCN-GNDQLCVGRGPGYPDEILSTKIIGERQFKSAVELFQSASEEVKGKIEYSHVYLNFTDIEVELESNKVVKTCPAALGPGFAAGTTDGPGAFGFQQGDLKINPFWKNLRD-FLKEPSQYQVDCQNPKPVLLSTGEMFDPYAWAPAILPIQILRLGKLIILSVPGEFTTMAGRRLREAVKETLISSSNGEFNDETHVVIAGLTNTYSQYIATFEEYQHQRYEAASTLYGPHTLSAYIQEFKKLAQAMAEGK-NITTKGPSPPDLSSVQI-SLLLGPFGDSPPAGIEFGDIKEDIAFPERGYFRKGDTPSATFWSSNPRYD-LLTEGTFAAVERLQGE--RWIATYDDDDLSLFFKWKVDNSSMHGLATIEWEIPFGSVSGVYRLRHFGATRITI--TSPISYFTGASSAFAV 753
YLIGVG D TGP AD+ MMGYAN +Q G+H RL +R FIV + R VFV D M SQ L ++V + L ++G LY ++NV +SGTHTH G GGY QY ++ +TS G++K S I I +SI AH NL+P I++N G+V D NRSP +Y+ NP ER RY N D Q+ +LKF D + G+ IG +WFA H SM N++++ DN G A+ +FE ++G L + FVA F SN+GD SPN+ G C++TG CD+ +SSC G + C+ GPG D ST+IIGE FK A EL+ SA +E+ G + +H ++N TD V+ S +TC ALG FAAGTTDG G F F QGD + +PFW +RD L PS C PKP+L STGEM P W PAI+ +QI+ +G L +++VPGEFTTM+GRR+REAVK L E VV+AGL N Y+ YI T+EEYQ QRYE AST++GPHTLSAYIQ ++ LA+A+A G KGP PP ++ + + P D P G FGD+ +++ ++ G+ S TF S NPR+ + + T VER + W ++D F W + + A +EW IP + +G Y++++FG + T T+ I+ + G S+AF V
Sbjct: 61 YLIGVGRADCTGPVADLPMMGYANTDQTARGLHTRLFSRAFIVDDG--NKRVVFVTSDIGMVSQRLRLEVFQALKEKYGDLYRQDNVVLSGTHTHSGVGGYFQYTLFMITSKGYIKPSIQAIVSGIVKSIDIAHRNLRPGRIFINKGQVADSNFNRSPHSYMNNPEEERNRYEFNTDKQIVVLKFTDLD-GDGIGLLSWFAVHPVSMNYTNRMVSSDNLGYASYIFE--------------QEKNIGFL--------------------------PGEKGPFVAGFSSSNLGDSSPNIRGPVCVNTGLKCDYINSSCPVGGKKACIAFGPG-EDMFESTRIIGENMFKIAKELYGSAKQELHGPVYGAHQWVNMTDETVQFNSTHTGRTCKPALGHSFAAGTTDGGGEFNFLQGDTEGDPFWDGIRDAVLGPPSNETKACHQPKPILFSTGEMDSPLPWHPAIVDVQIITIGSLAVVAVPGEFTTMSGRRIREAVKREL---EVKEPFTNAEVVVAGLCNIYTHYITTYEEYQIQRYEGASTIFGPHTLSAYIQRYRGLAKAIAHGTIGELPKGPEPPFFDEDKLFNQVRDPVADVAPVGTTFGDVLQEV----NPVYKVGEIASVTFVSGNPRHSGDIRDTTLVTVERFHNDTGSWEIIHNDASWETRFHW-IKGLAGRSQAKVEWHIPQTAQAGTYQIQYFGHYKQTTENTTVITPYVGTSAAFKV 736
BLAST of Vigst.05G166500.01 vs. ExPASy Swiss-Prot (2024)
Match: sp|Q54BK2|NCSEA_DICDI (Neutral ceramidase A OS=Dictyostelium discoideum OX=44689 GN=dcd2A PE=1 SV=1) HSP 1 Score: 537.339 bits (1383), Expect = 0.000e+0 Identity = 305/728 (41.90%), Postives = 423/728 (58.10%), Query Frame = 0
Query: 35 YLIGVGSYDMTGPAADVNMMGYANMEQNTAGIHFRLRARTFIVGESPDGPRFVFVNLDACMASQLLTIKVLERLNSRFG-KLYNEENVAISGTHTHGGPGGYMQYLVYSVTSLGFVKQSFDVIADAIEQSIIQAHNNLKPASIYLNIGEVKDGGINRSPSAYLQNPANERARYASNVDTQMTLLKFVDEESGESIGAFTWFATHGTSMTNKNKLITGDNKGVAARLFEDWFASQNTSSTTNSSVSDVGQLVKMAQSIKATRGKDCSKQASKASKVRKNDGSLFVAAFCQSNVGDVSPNVLGAFCIDTGKPCDFNHSSCNGNDQLCVGRGPGYPDEIL-STKIIGERQFKSAVELFQSASEEVKGKIEYSHVYLNFTDIEVELESNKVVK---TCPAALGPGFAAGTTDGPGAFGFQQGD-LKINPFWKNLRDFLKEPSQYQVDCQNPKPVLLSTGEMFDPYAWAPAILPIQILRLGKLIILSVPGEFTTMAGRRLREAVKETLISSSNGEFNDETHVVIAGLTNTYSQYIATFEEYQHQRYEAASTLYGPHTLSAYIQEFKKLAQAMAEGKNITTKGPSPPDLSSVQISLLLGPFGDSPPAGIEFGDIKEDIAFPERGYFRKGDTPSATFWSSNPRYDLLTEGTFAAVERLQGE-RWIATYDDDDLSLFFKWKVDNSSMHGLATIEWEIPFGSVSGVYRLRHFG-ATRITITSPISYFTGASSAFAVQ 754
Y IG G YD+TGP A+ NMMGYA Q T GIHFR RAR F+ +S +G R V+V+ D+CM Q + I+V++ L FG LY +NV +SGTHTH GP G+ +Y +Y +T+LGF K++FD I D I Q+I++AH +++PA + GE+ + INRSP AY NP E+A Y +NVD MT+++ +++ SG A ++F H TSM N N LI+GDNKG A+ L+E Q SS+ G F+AAF QSN GDVSPN G C D GKPCD+ S+CNG + C GPG ++ ST+IIG QF A+ELF +A+ +V GKI+Y H + FT++ VE N V+ TC A+G FA GTTDGPGAF F QGD NPFW + + +P+ Q CQ PKP+L+ G M +P W P ++P+QI+ LG++++++VPGEFTTM+GRRLR V+E + G+ + V+IAGL NTYS YIATFEE+Q QRYE AST++GPHTL AY QEF LAQ++ +G G P ++S L D P +FGDI D++ + T + F+ +N R + +TE +F V++LQ +W +D D WK+ + L T++W I + G YR+ H G A + + ++++ G SS F VQ
Sbjct: 40 YQIGTGIYDITGPGAETNMMGYAMPGQITGGIHFRQRARAFVFIDS-EGNRAVYVSTDSCMIFQEVKIQVIQDLQEIFGPTLYTHDNVLLSGTHTHSGPAGFSEYALYGITALGFYKKNFDTICDGIVQAIVKAHKSVQPARMLTQQGELWNSNINRSPYAYDNNPEEEKAMYDANVDKNMTVIR-IEDMSGNPFAAISFFGVHCTSMNNTNHLISGDNKGYASYLWEKHANGQ-------SSLPGTGP---------------------------------FIAAFGQSNEGDVSPNTRGPTCRD-GKPCDYKTSTCNGKVEECWALGPGTDGDMFESTQIIGGNQFNKALELFNNATIQVSGKIQYRHTWKPFTNVSVEAPYNSGVEGATTCRGAMGYSFAGGTTDGPGAFNFIQGDNSTTNPFWNFIGGIIAKPTPQQTACQAPKPILIDVG-MVEPIPWVPDVMPLQIITLGQIVLVAVPGEFTTMSGRRLRNTVREII-----GQSIENPIVLIAGLANTYSGYIATFEEFQVQRYEGASTVFGPHTLGAYQQEFANLAQSIVDGSQ-ADPGTFPRNMSGHTPFFLPPVIVDVAPKFDDFGDIYTDVSTTTP--YSINQTVTVIFYGANLRNNFMTESSFLTVDQLQSNGQWTTILNDGDWDTKLYWKMHDLGF-SLITVDWTISPITQPGTYRITHSGYAKKNPFSDNLTFYQGISSNFNVQ 714
BLAST of Vigst.05G166500.01 vs. Araport11
Match: | (Symbols: AtNCER2 | neutral ceramidase 2 | chr2:15906862-15909867 FORWARD LENGTH=757) HSP 1 Score: 904.049 bits (2335), Expect = 0.000e+0 Identity = 438/718 (61.00%), Postives = 542/718 (75.49%), Query Frame = 0
Query: 35 YLIGVGSYDMTGPAADVNMMGYANMEQNTAGIHFRLRARTFIVGESPDGPRFVFVNLDACMASQLLTIKVLERLNSRFGKLYNEENVAISGTHTHGGPGGYMQYLVYSVTSLGFVKQSFDVIADAIEQSIIQAHNNLKPASIYLNIGEVKDGGINRSPSAYLQNPANERARYASNVDTQMTLLKFVDEESGESIGAFTWFATHGTSMTNKNKLITGDNKGVAARLFEDWF--------ASQNTSSTTNSSVSDVGQ----LVKMAQSIKATRGKDCSKQASKASKVRKNDGSLFVAAFCQSNVGDVSPNVLGAFCIDTGKPCDFNHSSCNGNDQLCVGRGPGYPDEILSTKIIGERQFKSAVELFQSASEEVKGKIEYSHVYLNFTDIEVELES----NKVVKTCPAALGPGFAAGTTDGPGAFGFQQGDLKINPFWKNLRDFLKEPSQYQVDCQNPKPVLLSTGEMFDPYAWAPAILPIQILRLGKLIILSVPGEFTTMAGRRLREAVKETLISSSNGEFNDETHVVIAGLTNTYSQYIATFEEYQHQRYEAASTLYGPHTLSAYIQEFKKLAQAMAEGKNITTKGPSPPDLSSVQISLLLGPFGDSPPAGIEFGDIKEDIAFPERGYFRKGDTPSATFWSSNPRYDLLTEGTFAAVERL-QGERWIATYDDDDLSLFFKW-KVDNSSMHGLATIEWEIPFGSVSGVYRLRHFGATR 734
YLIGVGSYD+TGPAADVNMMGYAN +Q +GIHFRLRAR FIV E P G R VFVNLDACMASQ++TIKVLERL +R+G+LY E+NVAISG HTH GPGGY+QY+ Y VTSLGFV+QSFDV+ + IEQSI+QAH +L+P S ++N G++ D G+NRSPS+YL NPA ER++Y +VD +MTL+KFVD + G + G+F WFATHGTSM+ N LI+GDNKG AAR EDWF +S+N ++ VSD + L+ +A + K++RG K ++VR FV+AFCQSN GDVSPN LG FCIDTG PCDFNHS+CNG ++LC GRGPGYPDE ST+IIGE+QFK AVELF A+E+++GKI Y H YL+F++++V + ++ VKTCPAA+G GFAAGTTDGPGAF F+QGD + N FW+ +R+ L+ P QV CQ PKP+LL TGEM +PY WAP+ILPIQILR+G+L+ILSVPGEFTTMAGRRLR+A+K LISS EF++ HVVIAGLTNTYSQYIATFEEY+ QRYE ASTLYG HTL+AYIQEFKKLA A+ G + +GP PPDL QISLL DS P G++FGD+K D+ P + FR+G +ATFWS PR DL+TEG+FA VE L +G +W YDDDD SL FKW + S ATIEW +P +V+GVYR+RH+GA++
Sbjct: 26 YLIGVGSYDITGPAADVNMMGYANSDQIASGIHFRLRARAFIVAE-PQGNRVVFVNLDACMASQIVTIKVLERLKARYGELYTEKNVAISGIHTHAGPGGYLQYVTYIVTSLGFVRQSFDVVVNGIEQSIVQAHESLRPGSAFVNKGDLLDAGVNRSPSSYLNNPAAERSKYKYDVDKEMTLVKFVDSQLGPT-GSFNWFATHGTSMSRTNSLISGDNKGAAARFMEDWFENGQKNSVSSRNIPRRVSTIVSDFSRNQSRLLDIAATYKSSRGHSVDKSLDVKTRVRNGSKRKFVSAFCQSNCGDVSPNTLGTFCIDTGLPCDFNHSTCNGQNELCYGRGPGYPDEFESTRIIGEKQFKMAVELFNKATEKLQGKIGYQHAYLDFSNLDVTVPKAGGGSETVKTCPAAMGFGFAAGTTDGPGAFDFKQGDDQGNVFWRLVRNVLRTPGPEQVQCQKPKPILLDTGEMKEPYDWAPSILPIQILRIGQLVILSVPGEFTTMAGRRLRDAIKSFLISSDPKEFSNNMHVVIAGLTNTYSQYIATFEEYEVQRYEGASTLYGRHTLTAYIQEFKKLATALVNGLTL-PRGPQPPDLLDKQISLLSPVVVDSTPLGVKFGDVKADV--PPKSTFRRGQQVNATFWSGCPRNDLMTEGSFAVVETLREGGKWAPVYDDDDFSLKFKWSRPAKLSSESQATIEWRVPESAVAGVYRIRHYGASK 738
BLAST of Vigst.05G166500.01 vs. Araport11
Match: | (Symbols: AtNCER2 | neutral ceramidase 2 | chr2:15906862-15909867 FORWARD LENGTH=757) HSP 1 Score: 903.664 bits (2334), Expect = 0.000e+0 Identity = 438/718 (61.00%), Postives = 542/718 (75.49%), Query Frame = 0
Query: 35 YLIGVGSYDMTGPAADVNMMGYANMEQNTAGIHFRLRARTFIVGESPDGPRFVFVNLDACMASQLLTIKVLERLNSRFGKLYNEENVAISGTHTHGGPGGYMQYLVYSVTSLGFVKQSFDVIADAIEQSIIQAHNNLKPASIYLNIGEVKDGGINRSPSAYLQNPANERARYASNVDTQMTLLKFVDEESGESIGAFTWFATHGTSMTNKNKLITGDNKGVAARLFEDWF--------ASQNTSSTTNSSVSDVGQ----LVKMAQSIKATRGKDCSKQASKASKVRKNDGSLFVAAFCQSNVGDVSPNVLGAFCIDTGKPCDFNHSSCNGNDQLCVGRGPGYPDEILSTKIIGERQFKSAVELFQSASEEVKGKIEYSHVYLNFTDIEVELES----NKVVKTCPAALGPGFAAGTTDGPGAFGFQQGDLKINPFWKNLRDFLKEPSQYQVDCQNPKPVLLSTGEMFDPYAWAPAILPIQILRLGKLIILSVPGEFTTMAGRRLREAVKETLISSSNGEFNDETHVVIAGLTNTYSQYIATFEEYQHQRYEAASTLYGPHTLSAYIQEFKKLAQAMAEGKNITTKGPSPPDLSSVQISLLLGPFGDSPPAGIEFGDIKEDIAFPERGYFRKGDTPSATFWSSNPRYDLLTEGTFAAVERL-QGERWIATYDDDDLSLFFKW-KVDNSSMHGLATIEWEIPFGSVSGVYRLRHFGATR 734
YLIGVGSYD+TGPAADVNMMGYAN +Q +GIHFRLRAR FIV E P G R VFVNLDACMASQ++TIKVLERL +R+G+LY E+NVAISG HTH GPGGY+QY+ Y VTSLGFV+QSFDV+ + IEQSI+QAH +L+P S ++N G++ D G+NRSPS+YL NPA ER++Y +VD +MTL+KFVD + G + G+F WFATHGTSM+ N LI+GDNKG AAR EDWF +S+N ++ VSD + L+ +A + K++RG K ++VR FV+AFCQSN GDVSPN LG FCIDTG PCDFNHS+CNG ++LC GRGPGYPDE ST+IIGE+QFK AVELF A+E+++GKI Y H YL+F++++V + ++ VKTCPAA+G GFAAGTTDGPGAF F+QGD + N FW+ +R+ L+ P QV CQ PKP+LL TGEM +PY WAP+ILPIQILR+G+L+ILSVPGEFTTMAGRRLR+A+K LISS EF++ HVVIAGLTNTYSQYIATFEEY+ QRYE ASTLYG HTL+AYIQEFKKLA A+ G + +GP PPDL QISLL DS P G++FGD+K D+ P + FR+G +ATFWS PR DL+TEG+FA VE L +G +W YDDDD SL FKW + S ATIEW +P +V+GVYR+RH+GA++
Sbjct: 26 YLIGVGSYDITGPAADVNMMGYANSDQIASGIHFRLRARAFIVAE-PQGNRVVFVNLDACMASQIVTIKVLERLKARYGELYTEKNVAISGIHTHAGPGGYLQYVTYIVTSLGFVRQSFDVVVNGIEQSIVQAHESLRPGSAFVNKGDLLDAGVNRSPSSYLNNPAAERSKYKYDVDKEMTLVKFVDSQLGPT-GSFNWFATHGTSMSRTNSLISGDNKGAAARFMEDWFENGQKNSVSSRNIPRRVSTIVSDFSRNRDRLLDIAATYKSSRGHSVDKSLDVKTRVRNGSKRKFVSAFCQSNCGDVSPNTLGTFCIDTGLPCDFNHSTCNGQNELCYGRGPGYPDEFESTRIIGEKQFKMAVELFNKATEKLQGKIGYQHAYLDFSNLDVTVPKAGGGSETVKTCPAAMGFGFAAGTTDGPGAFDFKQGDDQGNVFWRLVRNVLRTPGPEQVQCQKPKPILLDTGEMKEPYDWAPSILPIQILRIGQLVILSVPGEFTTMAGRRLRDAIKSFLISSDPKEFSNNMHVVIAGLTNTYSQYIATFEEYEVQRYEGASTLYGRHTLTAYIQEFKKLATALVNGLTL-PRGPQPPDLLDKQISLLSPVVVDSTPLGVKFGDVKADV--PPKSTFRRGQQVNATFWSGCPRNDLMTEGSFAVVETLREGGKWAPVYDDDDFSLKFKWSRPAKLSSESQATIEWRVPESAVAGVYRIRHYGASK 738
BLAST of Vigst.05G166500.01 vs. Araport11
Match: | (Symbols: AtNCER2 | neutral ceramidase 2 | chr2:15906862-15909867 FORWARD LENGTH=792) HSP 1 Score: 890.567 bits (2300), Expect = 0.000e+0 Identity = 438/753 (58.17%), Postives = 542/753 (71.98%), Query Frame = 0
Query: 35 YLIGVGSYDMTGPAADVNMMGYANMEQNTAGIHFRLRARTFIVGESPDGPRFVFVNLDACMASQLLTIKVLERLNSRFGKLYNEENVAISGTHTHGGPGGYMQYLVYSVTSLGFVKQSFDVIADAIEQSIIQAHNNLKPASIYLNIGEVKDGGINRSPSAYLQNPANERARYASNVDTQMTLLKFVDEESGESIGAFTWFATHGTSMTNKNKLITGDNKGVAARLFEDWF--------ASQNTSSTTNSSVSDVGQ---------------------------------------LVKMAQSIKATRGKDCSKQASKASKVRKNDGSLFVAAFCQSNVGDVSPNVLGAFCIDTGKPCDFNHSSCNGNDQLCVGRGPGYPDEILSTKIIGERQFKSAVELFQSASEEVKGKIEYSHVYLNFTDIEVELES----NKVVKTCPAALGPGFAAGTTDGPGAFGFQQGDLKINPFWKNLRDFLKEPSQYQVDCQNPKPVLLSTGEMFDPYAWAPAILPIQILRLGKLIILSVPGEFTTMAGRRLREAVKETLISSSNGEFNDETHVVIAGLTNTYSQYIATFEEYQHQRYEAASTLYGPHTLSAYIQEFKKLAQAMAEGKNITTKGPSPPDLSSVQISLLLGPFGDSPPAGIEFGDIKEDIAFPERGYFRKGDTPSATFWSSNPRYDLLTEGTFAAVERL-QGERWIATYDDDDLSLFFKW-KVDNSSMHGLATIEWEIPFGSVSGVYRLRHFGATR 734
YLIGVGSYD+TGPAADVNMMGYAN +Q +GIHFRLRAR FIV E P G R VFVNLDACMASQ++TIKVLERL +R+G+LY E+NVAISG HTH GPGGY+QY+ Y VTSLGFV+QSFDV+ + IEQSI+QAH +L+P S ++N G++ D G+NRSPS+YL NPA ER++Y +VD +MTL+KFVD + G + G+F WFATHGTSM+ N LI+GDNKG AAR EDWF +S+N ++ VSD + L+ +A + K++RG K ++VR FV+AFCQSN GDVSPN LG FCIDTG PCDFNHS+CNG ++LC GRGPGYPDE ST+IIGE+QFK AVELF A+E+++GKI Y H YL+F++++V + ++ VKTCPAA+G GFAAGTTDGPGAF F+QGD + N FW+ +R+ L+ P QV CQ PKP+LL TGEM +PY WAP+ILPIQILR+G+L+ILSVPGEFTTMAGRRLR+A+K LISS EF++ HVVIAGLTNTYSQYIATFEEY+ QRYE ASTLYG HTL+AYIQEFKKLA A+ G + +GP PPDL QISLL DS P G++FGD+K D+ P + FR+G +ATFWS PR DL+TEG+FA VE L +G +W YDDDD SL FKW + S ATIEW +P +V+GVYR+RH+GA++
Sbjct: 26 YLIGVGSYDITGPAADVNMMGYANSDQIASGIHFRLRARAFIVAE-PQGNRVVFVNLDACMASQIVTIKVLERLKARYGELYTEKNVAISGIHTHAGPGGYLQYVTYIVTSLGFVRQSFDVVVNGIEQSIVQAHESLRPGSAFVNKGDLLDAGVNRSPSSYLNNPAAERSKYKYDVDKEMTLVKFVDSQLGPT-GSFNWFATHGTSMSRTNSLISGDNKGAAARFMEDWFENGQKNSVSSRNIPRRVSTIVSDFSRNRESYPFLELPNVAILYIAVAERLMWKVMVLFYFVSESRLLDIAATYKSSRGHSVDKSLDVKTRVRNGSKRKFVSAFCQSNCGDVSPNTLGTFCIDTGLPCDFNHSTCNGQNELCYGRGPGYPDEFESTRIIGEKQFKMAVELFNKATEKLQGKIGYQHAYLDFSNLDVTVPKAGGGSETVKTCPAAMGFGFAAGTTDGPGAFDFKQGDDQGNVFWRLVRNVLRTPGPEQVQCQKPKPILLDTGEMKEPYDWAPSILPIQILRIGQLVILSVPGEFTTMAGRRLRDAIKSFLISSDPKEFSNNMHVVIAGLTNTYSQYIATFEEYEVQRYEGASTLYGRHTLTAYIQEFKKLATALVNGLTL-PRGPQPPDLLDKQISLLSPVVVDSTPLGVKFGDVKADV--PPKSTFRRGQQVNATFWSGCPRNDLMTEGSFAVVETLREGGKWAPVYDDDDFSLKFKWSRPAKLSSESQATIEWRVPESAVAGVYRIRHYGASK 773
BLAST of Vigst.05G166500.01 vs. Araport11
Match: | (Symbols: AtNCER1 | neutral ceramidase 1 | chr1:2264829-2268309 REVERSE LENGTH=780) HSP 1 Score: 859.751 bits (2220), Expect = 0.000e+0 Identity = 437/753 (58.03%), Postives = 544/753 (72.24%), Query Frame = 0
Query: 31 THGEYLIGVGSYDMTGPAADVNMMGYANMEQNTAGIHFRLRARTFIVGESPDGPRFVFVNLDACMASQLLTIKVLERLNSRFGKLYNEENVAISGTHTHGGPGGYMQYLVYSVTSLGFVKQSFDVIADAIEQSIIQAHNNLKPASIYLNIGEVKDGGINRSPSAYLQNPANERARYASNVDTQMTLLKFVDEESGESIGAFTWFATHGTSMTNKNKLITGDNKGVAARLFEDWFASQNTSSTTNSS--VSDV----------------GQLVKMAQSIKATRGKDCSKQASKASKVR----KNDGSLFVAAFCQSNVGDVSPNVLGAFCIDTGKPCDFNHSSCNGNDQLCVGRGPGYPDEILSTKIIGERQFKSAVELFQSASEEVKGKIEYSHVYLNFTDIEVELES----NKVVKTCPAALGPGFAAGTTDGPGAFGFQQGDLKINPFWKNLRDFLKEPSQYQVDCQNPKPVLLSTGEMFDPYAWAPAILPIQILRLGKLIILSVPGEFTTMAGRRLREAVKETLISSSNGEFNDETHVVIAGLTNTYSQYIATFEEYQHQRYEAASTLYGPHTLSAYIQEFKKLAQAMAEGKNITTKGPSPPDLSSVQISLLLGPFGDSPPAGIEFGDIKEDIAFPERGYFRKGD-TPSATFWSSNPRYDLLTEGTFAAVERLQ--GERWIATYDDDDLSLFFKW-KVDNSSMHGLATIEWEIPFGSVSGVYRLRHFGATRITITSPISYFTGASSAFAV 753
+H EYLIG+GSYD+TGPAADVNMMGYANMEQ +GIHFRLRARTFIV E P G R VFVNLDACMASQ++ +KV+ERL +R+G LY E+NV ISG HTH GPGGY+QY+VY VTSLGFV+QSFD + D IE SIIQAH NL+P SI+LN GE+ D G+NRSPSAYL NP+ ER+++ NVD +MTLLKFVD++ G +G+F WFATHGTSM+ N LI+GDNKG A+R EDW+ QNT+ + S +SD +L+++A ++ GK ++ +S A +VR K D FV+AFCQ+N GDVSPNVLGAFC+DTG PCDFNHS+C G +++C GRGPGYPDE ST+IIGERQFK A+ELF ASE+++GK++Y HVY++F+ + V L ++VVKTCPAA+G FAAGTTDGPGAF F QGD K NPFW+ +R+ LK P + Q+DC PKP+LL TGEM PY WAP+IL +Q+LR+G+L ILSVPGEFTTMAGRRLR AVK L +S N + + E HVVIAGL N YSQY+ TFEEYQ QRYE ASTLYGPHTLS YIQEFKKL++++ + GP PPDL Q+S L D+ P+G FGD+ D+ P+ ++G+ + F S+ PR DLLTEGTF VERL+ + W YDDDDL L FKW + S AT+EW IP + GVYR+ HFGA + S + +FTG+SSAF V
Sbjct: 34 SHSEYLIGLGSYDITGPAADVNMMGYANMEQVASGIHFRLRARTFIVSE-PQGKRVVFVNLDACMASQIVKLKVIERLKARYGDLYTEQNVGISGIHTHAGPGGYLQYVVYIVTSLGFVRQSFDALVDGIENSIIQAHENLRPGSIFLNNGELLDAGVNRSPSAYLNNPSKERSKHKYNVDKEMTLLKFVDDQWG-PVGSFNWFATHGTSMSRTNSLISGDNKGAASRFMEDWY-EQNTAERSYSEEFISDEIPRRVSSLIENHQDSHHELLELASYFESQPGKPVTRISSSARRVRSALRKADKPGFVSAFCQTNCGDVSPNVLGAFCLDTGLPCDFNHSTCGGKNEMCYGRGPGYPDEFESTRIIGERQFKMALELFNKASEQLQGKVDYRHVYVDFSQLNVTLPKKDGKSEVVKTCPAAMGFAFAAGTTDGPGAFDFTQGDDKGNPFWRLVRNVLKTPDKKQIDCHYPKPILLDTGEMTKPYDWAPSILSLQVLRIGQLFILSVPGEFTTMAGRRLRYAVKTQLKNSGNKDLSGEIHVVIAGLANGYSQYVTTFEEYQVQRYEGASTLYGPHTLSGYIQEFKKLSKSLVLDMPV-QPGPQPPDLLDKQLSFLTPVMMDTTPSGDSFGDVISDV--PKNLSLKRGNGQVTVVFRSACPRNDLLTEGTFTLVERLEQKDKTWTPVYDDDDLCLRFKWSRHKKLSSRSQATVEWRIPESASPGVYRITHFGAAKKLFGS-VHHFTGSSSAFVV 779
BLAST of Vigst.05G166500.01 vs. Araport11
Match: | (Symbols: AtNCER1 | neutral ceramidase 1 | chr1:2264829-2268306 REVERSE LENGTH=779) HSP 1 Score: 859.366 bits (2219), Expect = 0.000e+0 Identity = 437/753 (58.03%), Postives = 544/753 (72.24%), Query Frame = 0
Query: 31 THGEYLIGVGSYDMTGPAADVNMMGYANMEQNTAGIHFRLRARTFIVGESPDGPRFVFVNLDACMASQLLTIKVLERLNSRFGKLYNEENVAISGTHTHGGPGGYMQYLVYSVTSLGFVKQSFDVIADAIEQSIIQAHNNLKPASIYLNIGEVKDGGINRSPSAYLQNPANERARYASNVDTQMTLLKFVDEESGESIGAFTWFATHGTSMTNKNKLITGDNKGVAARLFEDWFASQNTSSTTNSS--VSDV----------------GQLVKMAQSIKATRGKDCSKQASKASKVR----KNDGSLFVAAFCQSNVGDVSPNVLGAFCIDTGKPCDFNHSSCNGNDQLCVGRGPGYPDEILSTKIIGERQFKSAVELFQSASEEVKGKIEYSHVYLNFTDIEVELES----NKVVKTCPAALGPGFAAGTTDGPGAFGFQQGDLKINPFWKNLRDFLKEPSQYQVDCQNPKPVLLSTGEMFDPYAWAPAILPIQILRLGKLIILSVPGEFTTMAGRRLREAVKETLISSSNGEFNDETHVVIAGLTNTYSQYIATFEEYQHQRYEAASTLYGPHTLSAYIQEFKKLAQAMAEGKNITTKGPSPPDLSSVQISLLLGPFGDSPPAGIEFGDIKEDIAFPERGYFRKGD-TPSATFWSSNPRYDLLTEGTFAAVERLQ--GERWIATYDDDDLSLFFKW-KVDNSSMHGLATIEWEIPFGSVSGVYRLRHFGATRITITSPISYFTGASSAFAV 753
+H EYLIG+GSYD+TGPAADVNMMGYANMEQ +GIHFRLRARTFIV E P G R VFVNLDACMASQ++ +KV+ERL +R+G LY E+NV ISG HTH GPGGY+QY+VY VTSLGFV+QSFD + D IE SIIQAH NL+P SI+LN GE+ D G+NRSPSAYL NP+ ER+++ NVD +MTLLKFVD++ G +G+F WFATHGTSM+ N LI+GDNKG A+R EDW+ QNT+ + S +SD +L+++A ++ GK ++ +S A +VR K D FV+AFCQ+N GDVSPNVLGAFC+DTG PCDFNHS+C G +++C GRGPGYPDE ST+IIGERQFK A+ELF ASE+++GK++Y HVY++F+ + V L ++VVKTCPAA+G FAAGTTDGPGAF F QGD K NPFW+ +R+ LK P + Q+DC PKP+LL TGEM PY WAP+IL +Q+LR+G+L ILSVPGEFTTMAGRRLR AVK L +S N + + E HVVIAGL N YSQY+ TFEEYQ QRYE ASTLYGPHTLS YIQEFKKL++++ + GP PPDL Q+S L D+ P+G FGD+ D+ P+ ++G+ + F S+ PR DLLTEGTF VERL+ + W YDDDDL L FKW + S AT+EW IP + GVYR+ HFGA + S + +FTG+SSAF V
Sbjct: 33 SHSEYLIGLGSYDITGPAADVNMMGYANMEQVASGIHFRLRARTFIVSE-PQGKRVVFVNLDACMASQIVKLKVIERLKARYGDLYTEQNVGISGIHTHAGPGGYLQYVVYIVTSLGFVRQSFDALVDGIENSIIQAHENLRPGSIFLNNGELLDAGVNRSPSAYLNNPSKERSKHKYNVDKEMTLLKFVDDQWG-PVGSFNWFATHGTSMSRTNSLISGDNKGAASRFMEDWY-EQNTAERSYSEEFISDEIPRRVSSLIENHQDSHHELLELASYFESQPGKPVTRISSSARRVRSALRKADKPGFVSAFCQTNCGDVSPNVLGAFCLDTGLPCDFNHSTCGGKNEMCYGRGPGYPDEFESTRIIGERQFKMALELFNKASEQLQGKVDYRHVYVDFSQLNVTLPKKDGKSEVVKTCPAAMGFAFAAGTTDGPGAFDFTQGDDKGNPFWRLVRNVLKTPDKKQIDCHYPKPILLDTGEMTKPYDWAPSILSLQVLRIGQLFILSVPGEFTTMAGRRLRYAVKTQLKNSGNKDLSGEIHVVIAGLANGYSQYVTTFEEYQVQRYEGASTLYGPHTLSGYIQEFKKLSKSLVLDMPV-QPGPQPPDLLDKQLSFLTPVMMDTTPSGDSFGDVISDV--PKNLSLKRGNGQVTVVFRSACPRNDLLTEGTFTLVERLEQKDKTWTPVYDDDDLCLRFKWSRHKKLSSRSQATVEWRIPESASPGVYRITHFGAAKKLFGS-VHHFTGSSSAFVV 778
BLAST of Vigst.05G166500.01 vs. Araport11
Match: | (Symbols: AtNCER3 | Neutral Ceramidase3 | chr5:23811449-23814395 REVERSE LENGTH=733) HSP 1 Score: 835.484 bits (2157), Expect = 0.000e+0 Identity = 430/742 (57.95%), Postives = 522/742 (70.35%), Query Frame = 0
Query: 34 EYLIGVGSYDMTGPAADVNMMGYANMEQNTAGIHFRLRARTFIVGESPDGPRFVFVNLDACMASQLLTIKVLERLNSRFGKLYNEENVAISGTHTHGGPGGYMQYLVYSVTSLGFVKQSFDVIADAIEQSIIQAHNNLKPASIYLNIGEVKDGGINRSPSAYLQNPANERARYASNVDTQMTLLKFVDEESGESIGAFTWFATHGTSMTNKNKLITGDNKGVAARLFEDWFASQNTSSTTN--------SSVSDV--GQLVKMAQSIKATRGKDCSKQASKASKVRKN----DGSLFVAAFCQSNVGDVSPNVLGAFCIDTGKPCDFNHSSCNGNDQLCVGRGPGYPDEILSTKIIGERQFKSAVELFQSASEEVKGKIEYSHVYLNFTDIEVELE----SNKVVKTCPAALGPGFAAGTTDGPGAFGFQQGDLKINPFWKNLRDFLKEPSQYQVDCQNPKPVLLSTGEMFDPYAWAPAILPIQILRLGKLIILSVPGEFTTMAGRRLREAVKETLISSSNGEFNDETHVVIAGLTNTYSQYIATFEEYQHQRYEAASTLYGPHTLSAYIQEFKKLAQAMAEGKNITTKGPSPPDLSSVQISLLLGPFGDSPPAGIEFGDIKEDIAFPERGYFRKG-DTPSATFWSSNPRYDLLTEGTFAAVER-LQG-ERWIATYDDDDLSLFFKW-KVDNSSMHGLATIEWEIPFGSVSGVYRLRHFGATRITITSPISYFTGASSAFAV 753
+YL+G+GSYD+TGPAADVNMMGYANMEQ +G+HFRLRAR FIV E P R FVNLDA MASQL+TIKV+ERL R+G+LY EENVAISGTHTH GPGGY+QY++Y VTSLGFV QSF+ + D IEQSIIQAH NL+P SI +N GE+ D G+NRSPSAYL NPA+ER++Y +VD +MTL+KFVD++ W G AR+ EDWF +N + + S +SD L++MA S+ +T GK ++ +S A +VR D FV+AFCQ+N GDVSPNVLGAFCIDTG PC+FN S+C G ++ C GRGPGYPDE ST+IIGERQFK A +LF ASEE++GK++Y H Y++F+ +EV + ++VVKTCPAA+G GFAAGTTDGPGAF F+QGD + NPFW+ +R+ LK P++ QV CQ PKP+LL TGEM PY WAP+ILP+QILR+G+L+IL VPGEFTTMAGRRLR+AVK L SNG E VVIAGLTN+YSQYIATFEEYQ QRYE ASTLYGPHTLS YIQEFKKLA + + T GP PPDL QISLL D P G FGD+ D+ P FRKG D F S+NPR DL+TEGTFA VER L+G E W+ YDDDD L FKW + S ATIEW IP + GVYR+ HFG+ + I+S I +F+G+SSAF V
Sbjct: 28 DYLMGLGSYDITGPAADVNMMGYANMEQVASGVHFRLRARAFIVAE-PYKKRIAFVNLDAGMASQLVTIKVIERLKQRYGELYTEENVAISGTHTHAGPGGYLQYILYLVTSLGFVHQSFNALVDGIEQSIIQAHENLRPGSILINKGELLDAGVNRSPSAYLNNPAHERSKYEYDVDKEMTLVKFVDDQ---------W--------------------GPVARIMEDWFERENGCRSVDVESPRRVSSIISDPYDQDLMEMASSLLSTGGKTVTRMSSVARRVRSRFRHADKPRFVSAFCQTNCGDVSPNVLGAFCIDTGLPCEFNQSTCGGKNEQCYGRGPGYPDEFESTRIIGERQFKKAADLFTKASEEIQGKVDYRHAYVDFSQLEVTINGQNGGSEVVKTCPAAMGFGFAAGTTDGPGAFDFKQGDDQGNPFWRLVRNLLKNPTEEQVRCQRPKPILLDTGEMKQPYDWAPSILPVQILRIGQLVILCVPGEFTTMAGRRLRDAVKTVLKEGSNGR---EFSVVIAGLTNSYSQYIATFEEYQVQRYEGASTLYGPHTLSGYIQEFKKLANDLLSAQT-TDPGPQPPDLLHKQISLLTPVVADMTPIGTAFGDVTSDV--PRLSKFRKGADIVRVQFRSANPRNDLMTEGTFALVERWLEGRETWVPVYDDDDFCLRFKWSRPFKLSTQSTATIEWRIPETASPGVYRITHFGSAKTPISS-IHHFSGSSSAFVV 732 The following BLAST results are available for this feature:
BLAST of Vigst.05G166500.01 vs. ExPASy TrEMBL (2024)
Analysis Date: 2024-08-17 (Blastp of Vigna stipulacea acc. JP252948 v1.0 proteins vs UniProt TrEMBL) Total hits: 10
BLAST of Vigst.05G166500.01 vs. ExPASy Swiss-Prot (2024)
Analysis Date: 2024-08-17 (Blastp of Vigna stipulacea acc. JP252948 v1.0 proteins vs UniProt Swissprot) Total hits: 10
BLAST of Vigst.05G166500.01 vs. Araport11
Analysis Date: 2024-08-17 (Blastp of Vigna stipulacea acc. JP252948 v1.0 proteins vs arabidopsis Araport11) Total hits: 6
InterPro
Analysis Name: InterProScan Analysis for Vigna stipulacea acc. JP252948 genome v1.0
Date Performed: 2024-08-17
Sequences
The
following sequences are available for this feature:
mRNA sequence >drVigStip.JP252948.2.0.Vigst.05G166500.01 ID=drVigStip.JP252948.2.0.Vigst.05G166500.01; Name=Vigst.05G166500.01; organism=Vigna stipulacea; type=mRNA; length=2265bpback to top protein sequence of drVigStip.JP252948.2.0.Vigst.05G166500.01 >drVigStip.JP252948.2.0.Vigst.05G166500.01 ID=drVigStip.JP252948.2.0.Vigst.05G166500.01; Name=drVigStip.JP252948.2.0.Vigst.05G166500.01; organism=Vigna stipulacea; type=polypeptide; length=755bpback to top mRNA from alignment at Chr05:17020188..17038880- Legend: polypeptideexonCDS Hold the cursor over a type above to highlight its positions in the sequence below.>drVigStip.JP252948.2.0.Vigst.05G166500.01 ID=drVigStip.JP252948.2.0.Vigst.05G166500.01; Name=Vigst.05G166500.01; organism=Vigna stipulacea; type=mRNA; length=18693bp; location=Sequence derived from: Chr05:17020188..17038880- (Vigna stipulaceaback to top Coding sequence (CDS) from alignment at Chr05:17020188..17038880- >drVigStip.JP252948.2.0.Vigst.05G166500.01 ID=drVigStip.JP252948.2.0.Vigst.05G166500.01; Name=Vigst.05G166500.01; organism=Vigna stipulacea; type=CDS; length=2265bp; location=Sequence derived from: Chr05:17020188..17038880- (Vigna stipulaceaback to top Annotated Terms
The
following terms have been associated with
this mRNA:
|