Isolation and characterization of sequence-tagged microsatellite markers in chickpea (Cicer arietinum L.)
Publication Overview
Abstract In this study we report the isolation of microsatellite sequences and their conversion
to sequence-tagged microsatellite sites (STMS) markers in chickpea (
Cicer arietinum
L.).
Thirteen putative recombinants isolated from a chickpea genomic library were sequenced,
and used to design 10 STMS primer pairs. These were utilized to analyse the genetic
polymorphism in 15
C. arietinum
varieties and two wild varieties,
C. echinospermum
and
C. reticulatum
. All the primer pairs amplified polymorphic loci ranging from four to seven
alleles per locus. The observed heterozygosity ranged from 0 to 0.6667. Most of the STMS
markers also amplified corresponding loci in the wild relatives suggesting conservation
of these markers in the genus. Hence, these polymorphic markers will be useful for the
evaluation of genetic diversity and molecular mapping in chickpea.
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