Ca_04975, Ca_04975_v1.0_kabuli (mRNA) Cicer arietinum

BLAST of Ca_04975 vs. SwissProt
Match: RHIE_DICD3 (Rhamnogalacturonate lyase OS=Dickeya dadantii (strain 3937) GN=rhiE PE=1 SV=1)

HSP 1 Score: 115.546 bits (288), Expect = 2.324e-24
Identity = 143/592 (24.16%), Postives = 236/592 (39.86%), Query Frame = 1

Query: 1579 TLAELQVVMDNGILRVTLSNPEGIVTGIQYNGIDNLLEVLND-----ESNRGYWDLVWSSPTSTGTTGNFDVIKGSTFKVILEDEDQVELSFTRTWDASMEGKLVPLNIDKRFIMLSGCSGFYTYAIYEHLEDWPAFNLDETRIAFKLRKDKFHYMAMADNRQRNMPLPDDRVPP----RGQALAYPEAVLLVNPIEPELKGEVDDKYQYSCDNKDSQVHGWISMDPAVGFWLITPSNEFRSGGPVKQNLTSHVGPTTLAVFLSAHYSGQDLVPKFKAGEAWKKVFGPVFIYLNSPYDGGDPIKLWDDAKLQMLMEVQSWPYSFPESDDFPKWDERGYVSGRLLIKERCIDDDDYVSAKGAYVGLAPPGDVGSWQRECKNYQFWATADYDGYFSISNIHVGDYNVYAWVPG--YIGDYKYDVVISITEGCDIDIGDLVYEPLRDGPTLWEIGIPDRSAAEFYVPDPNPKYINKLYVNHPDKFRQYGIWERYAELYPDKDLIYTVGVSDYTKDWFFAQVTRKKDDNTYQGTTWQIKFKLDSVNRSSTYKLRVALASATYSELQVRVNDP----KANRPLFSSGLIGKDNSIAR 3309
            TL  +   +DNG+L+V     +G    + + G  NL+  L+      + NR ++   +S   +       +VIK        +  DQV L++         GKL    ++   IM    SG Y+Y +  +    P   + E R  ++   D      + ++ +R  PL  D +      + +    P+             G V  KY ++   ++S+   W  M    G W++  S E+ SG  +KQ L  H     L     +H+   D+V    A   ++K++GP  +Y+N     G+  +L  D   +   E  SWPY + +   +P+  +R  VSGRL               +  +  +       ++  +   Y F A  + DG FS+SN+  G+Y + A+  G   IG      V    EG    +G +  +  +  P  W IG  DR A EF                  DK RQY    R+    P  DL + +G S   KDW++AQ          Q  +W I F  ++      Y L +A+A+A+ + +    + P    K N  L ++     D SI R
Sbjct:   32 TLDGMNSTLDNGLLKVRFG-ADGSAKEV-WKGGTNLISRLSGAARDPDKNRSFYLDYYSGGVNEFVPERLEVIK--------QTPDQVHLAYID----DQNGKL---RLEYHLIMTRDVSGLYSYVVAANTGSAPV-TVSELRNVYRF--DATRLDTLFNSIRRGTPLLYDELEQLPKVQDETWRLPD-------------GSVYSKYDFAGYQRESRY--WGVMGNGYGAWMVPASGEYYSGDALKQELLVHQDAIILNYLTGSHFGTPDMV----AQPGFEKLYGPWLLYINQ----GNDRELVADVSRRAEHERASWPYRWLDDARYPR--QRATVSGRL-------------RTEAPHATVVLNSSAENFDIQTTGYLFSARTNRDGRFSLSNVPPGEYRLSAYADGGTQIGLLAQQTV--RVEGKKTRLGQI--DARQPAPLAWAIGQADRRADEF---------------RFGDKPRQY----RWQTEVP-ADLTFEIGKSRERKDWYYAQT---------QPGSWHILF--NTRTPEQPYTLNIAIAAASNNGMTTPASSPQLAVKLNGQLLTTLKYDNDKSIYR 530          

HSP 2 Score: 66.6254 bits (161), Expect = 1.238e-9
Identity = 64/209 (30.62%), Postives = 92/209 (44.02%), Query Frame = 1

Query: 1054 YQFWTRADENGNFTISNVRPGDYNLFAWVPGFVGDYNKFGDIMKIT---SGSYMELGQCIYKPPRDGPTLWEIGIPDRLAAEFYVPDPNPQHINKLFINHPDRFRQYGLWDRYSELYPDADLVYTIGVSDYRKDWFFAQVPRKREDNTHQGTTWQIKFELSGVIQGTTYTLRVALASATLAELQVVMDNGILRVTLSNPEGIVTGIQYN 1671
            Y F  R + +G F++SNV PG+Y L A+  G      + G + + T    G    LGQ   + P   P  W IG  DR A EF                  D+ RQY    R+    P ADL + IG S  RKDW++AQ          Q  +W I F      Q   YTL +A+A+A+   +     +  L V L+    ++T ++Y+
Sbjct:  354 YLFSARTNRDGRFSLSNVPPGEYRLSAYADG----GTQIGLLAQQTVRVEGKKTRLGQIDARQP--APLAWAIGQADRRADEF---------------RFGDKPRQY----RWQTEVP-ADLTFEIGKSRERKDWYYAQT---------QPGSWHILFNTRTPEQ--PYTLNIAIAAASNNGMTTPASSPQLAVKLNGQ--LLTTLKYD 523          

BLAST of Ca_04975 vs. SwissProt
Match: RGLB_EMENI (Rhamnogalacturonate lyase B OS=Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) GN=rglB PE=2 SV=2)

HSP 1 Score: 92.4337 bits (228), Expect = 2.107e-17
Identity = 134/606 (22.11%), Postives = 235/606 (38.78%), Query Frame = 1

Query: 1564 ALASATLAELQVVMDNGILRVTLSNPEGIVTGIQYNGIDNLLEVLNDESNRGYWDLVWSSPTSTGTTGNFDVIKGSTFKVILEDEDQVELSFTRTWDASMEGKLVPLN--IDKRFIMLSGCSGFYTYAIYEHLEDWPAF--NLDETRIAFKLRKDKFHYMAMADNRQRNMPLPDDRVPPRGQALAYPEAVLLVNPIEPELK----GEVDDKYQYSCDNKDSQVHGWI-----SMDPAVGFWLITPSNEFRSGGPVKQNLTSHVGPTTLAVFLSAHYSGQDLVPKFKAGEAWKKVFGPVFIYLNSPYDGGDPIKLWDDAKLQMLMEVQSWPYSFPESD-----DFPKWDERGYVSGRLLIKERCIDDDDYVSAKGAYV--GLAPPGDVGSWQRECKNYQFWATADYDGYFSISNIHVGDYNVYAWVPGYIGDYKYD-VVISITEGCDIDIGDLVYEPLRDGPTLWEIGIPDRSAAEF---YVPDPNPKYINKLYVNHPDKFRQY-GIWERYAELYPDKDLIYTVGVSDYTKDW---FFAQVTRKKDDNTYQGTT---WQIKFKLDS----VNRSSTYKLRVALASATYSELQV-RVNDPKANRPLFS 3273
            A  +AT  +  +V++N  LR T     G +  +  +G D L           Y D  + +P+   T G+ + +         E     + + T+     +     P      + + +  G +GF+ ++   +  +   F  NL E R  F+   D + ++  +D   +  PLP D      + +   +A   +N    +       E   KY +S   +D+ VHG       S     G WL+  + +   GGP+  +LT  V        +S H+   +  P    G  + + FGP F   N    GG    L +       +   SW   F +S       +    +RG V G + + +        ++  G Y     A P           ++Q+WA  D +G FSI  +  G Y +  +  G  GD+  D +V+   +   I      ++P   G  +W +G PD+S+ EF      DP        +  HP ++  Y G ++  ++     D  Y +G SD   D+    +A      D+   +  T   W+I+F L +      +++T  +++A A A      V   ++P AN PL S
Sbjct:   18 AALTATENDTSIVLENDRLRATFDKGRGSIIDLYLDGQDFLGPQSGSTGIGPYLDC-YCTPSGFYTAGSTNPVT--------ELVQGTDSTGTKYAGIILNDTYTPTGQEFQQYWFLRDGETGFHMFSRLAYYNETTPFLRNLQELRTLFRPNTDLWTHLTSSD--LQTAPLPSDEAI--AEQIVVQDATWRLNNTPDDAYYQQFSEYFTKYTFSNHWRDNDVHGLYADGSTSDGTTYGAWLVMNTKDTYYGGPLHSDLT--VDGIIYNYIVSNHHG--EGTPNITNG--FDRTFGPQFYLFN----GGGSSSLEELRDEARSLASPSWNADFYDSIAKHVIGYVPSSQRGSVKGTIKLPKNAKSPIAVLTVDGHYFQDNSAVPS----------SHQYWADIDKNGRFSIDRVVAGKYRLTVYADGIFGDFTRDGIVVKARKSTSI---KETWKPESAGTEIWRLGTPDKSSGEFRHGAARDPT-------HPRHPPEYLIYWGAYDWQSDFPGGID--YMIGESDPATDFNTVHWAVFGPTPDNPVAESNTTHDWRIRFDLSAKQLHARKTATLTIQLAGAKAASGNTDVYNASEPYANLPLRS 578          

HSP 2 Score: 70.4774 bits (171), Expect = 8.574e-11
Identity = 54/192 (28.12%), Postives = 86/192 (44.79%), Query Frame = 1

Query: 1048 ADYQFWTRADENGNFTISNVRPGDYNLFAWVPGFVGDYNKFGDIMKITSGSYMELGQCIYKPPRDGPTLWEIGIPDRLAAEF---YVPDP-NPQHINKLFINHPDRFRQYGLWDRYSELYPDADLVYTIGVSDYRKDW------FFAQVPRKREDNTHQGTTWQIKFELSG--VIQGTTYTLRVALASATLA 1587
            + +Q+W   D+NG F+I  V  G Y L  +  G  GD+ + G ++K    + ++     +KP   G  +W +G PD+ + EF      DP +P+H        P+    +G +D  S+     D  Y IG SD   D+       F   P      ++    W+I+F+LS   +    T TL + LA A  A
Sbjct:  379 SSHQYWADIDKNGRFSIDRVVAGKYRLTVYADGIFGDFTRDGIVVKARKSTSIKE---TWKPESAGTEIWRLGTPDKSSGEFRHGAARDPTHPRH-------PPEYLIYWGAYDWQSDFPGGID--YMIGESDPATDFNTVHWAVFGPTPDNPVAESNTTHDWRIRFDLSAKQLHARKTATLTIQLAGAKAA 558          

BLAST of Ca_04975 vs. SwissProt
Match: RGLB_ASPNC (Probable rhamnogalacturonate lyase B OS=Aspergillus niger (strain CBS 513.88 / FGSC A1513) GN=rglB PE=3 SV=1)

HSP 1 Score: 83.1889 bits (204), Expect = 1.278e-14
Identity = 132/593 (22.26%), Postives = 222/593 (37.44%), Query Frame = 1

Query: 1552 TLRVALASATLAELQ----VVMDNGILRVTLSNPEGIVTGIQYNGIDNLLEVLNDESNRGYWDLVW----------SSPTSTGTTGNFDVIKGSTFKVILEDEDQVELSFTRTWDASMEGKLVPLNIDKRFIMLSGCSGFYTYAIYEHLEDWPAF--NLDETRIAFKLRKDKFHYMAMADNRQRNMPLPDDRVPPRGQALAYPEAVLLVNPIEPELKGEVD--DKYQYSCDNKDSQVHGWI-----SMDPAVGFWLITPSNEFRSGGPVKQNLTSHVGPTTLAVFLSAHYSGQDLVPKFKAGEAWKKVFGPVFIYLNSPYDGGDPIKLWDDAKLQMLMEVQSWPYSFPESD-----DFPKWDERGYVSGRLLIKERCIDDDDYVSAKGAYVGLAPPGDVGSWQRECKNYQFWATADYDGYFSISNIHVGDYNVYAWVPGYIGDYKYDVVISITEGCDIDIGDLVYEPLRDGPTLWEIGIPDRSAAEF---YVPDPNPKYINKLYVNHPDKFRQYGIWERYAELYPDKDLIYTVGVSDYTKD-----WFFAQVTRKKDDNTYQGT-TWQIKFKL--DSVNRSSTYKLRVALASA 3213
            +L + L SATL   Q    + + N  L    S  +G +T +  +  D L     D     Y D             +SPT    TG      G+++  +L DE         T+  + +      +  + + +  G +G +T++   +  +   +  NL E R  F+   + + +++ ++   +  PLP  +       +          P +       D   KY +S   +D+ VHG       S     G WL+  + +   GGP+  +LT  V        +S H+   +  P    G  + + FGP + + N     G    L +       +   SW   F +S       +    +RG V G++ + +        ++  G Y        V S      +YQ+WA  D  G+FS+ ++  G Y +  +  G  GD+  D V  +  G    I +  +E    G  +W +G PD+S+ EF      DP        +  HP ++  Y     +   +PD  + YT+G SD   D     W     T       Y  T  W I F L  D +   S   L + LA A
Sbjct:   11 SLLLTLTSATLHTSQTNTTITLTNNRLTANFSKSQGRITDLYLDNQDLLGPQSGDTGVGPYLDCYCIPSGFYTPGSTSPTLQLFTGTDK--SGTSYAGVLMDE---------TYPPTGQ------HFQQYWFLRDGETGLHTFSRLAYYNETTPYLRNLQEFRTLFRPNTELWTHLSSSE--VQTAPLPSKKAVEEEVVVQDATWTFNNTPTDEYYVQFADYFTKYTFSNAWRDNSVHGMYADGSTSNGSTFGAWLVMNTKDTYYGGPLHSDLT--VDGIVYNYLVSNHHG--EGTPNITYG--FDRTFGPQYYHFNG--GKGSTASLQELKSDAETLADPSWNVDFYDSIAKHVVGYTPSSQRGSVQGKIKLPKGATRPIAVLTVDGQYF---QDNSVNS-----SSYQYWAEIDDSGHFSVDHVKEGPYRLTVYADGIFGDFVRDGV-QVKAGKKTTIQE-TWEAESAGTEIWRLGTPDKSSGEFRHGVARDPT-------HPLHPPEYLIYWGAYDWQSDFPD-GINYTIGTSDPATDLNTVHWSVFGPTPNDPRVEYDTTHDWTINFPLSEDDLAERSKATLTIQLAGA 558          

HSP 2 Score: 70.0922 bits (170), Expect = 1.120e-10
Identity = 55/192 (28.65%), Postives = 92/192 (47.92%), Query Frame = 1

Query: 1048 ADYQFWTRADENGNFTISNVRPGDYNLFAWVPGFVGDYNKFGDIMKITSGSYMELGQCIYKPPRDGPTLWEIGIPDRLAAEF-YVPDPNPQHINKLFINHPDRFRQYGLWDRYSELYPDADLVYTIGVSDYRKD-----WFF----AQVPRKREDNTHQGTTWQIKFELS--GVIQGTTYTLRVALASATLA 1587
            + YQ+W   D++G+F++ +V+ G Y L  +  G  GD+ + G  +++ +G    + Q  ++    G  +W +G PD+ + EF +    +P H     ++ P+    +G +D  S+ +PD  + YTIG SD   D     W         PR   D TH    W I F LS   + + +  TL + LA A  A
Sbjct:  382 SSYQYWAEIDDSGHFSVDHVKEGPYRLTVYADGIFGDFVRDG--VQVKAGKKTTI-QETWEAESAGTEIWRLGTPDKSSGEFRHGVARDPTHP----LHPPEYLIYWGAYDWQSD-FPDG-INYTIGTSDPATDLNTVHWSVFGPTPNDPRVEYDTTHD---WTINFPLSEDDLAERSKATLTIQLAGAKAA 561          

BLAST of Ca_04975 vs. SwissProt
Match: RGLB_ASPFN (Probable rhamnogalacturonate lyase B OS=Aspergillus flavus (strain ATCC 200026 / FGSC A1120 / NRRL 3357 / JCM 12722 / SRRC 167) GN=rglB PE=3 SV=1)

HSP 1 Score: 81.2629 bits (199), Expect = 4.857e-14
Identity = 133/604 (22.02%), Postives = 231/604 (38.25%), Query Frame = 1

Query: 1543 TTYTLRVALASATLAELQVVMDNGILRV-------TLSNPEGIVTGIQYNGIDNLLEVLNDESNRGYWDLVWSSPTSTGTTGNFD----VIKGSTFK------VILEDEDQVELSFTRTWDASMEGKLVPLNIDKRFIMLSGCSGFYTYAIYEHLEDWPAF--NLDETRIAFKLRKDKFHYMAMADNRQRNMPLPDDRVPPRGQALAYPEAVLLVNPIEPELK----GEVDDKYQYSCDNKDSQVHGWI-----SMDPAVGFWLITPSNEFRSGGPVKQNLTSHVGPTTLAVFLSAHYSGQDLVPKFKAGEAWKKVFGPVFIYLNSPYDGGDPIKLWDDAKLQMLMEVQSWPYSFPESD-----DFPKWDERGYVSGRLLIKERCIDDDDYVSAKGAYVGLAPPGDVGSWQRECKNYQFWATADYDGYFSISNIHVGDYNVYAWVPGYIGDYKYDVVISITEGCDIDIGDL-----VYEPLRDGPTLWEIGIPDRSAAEFYVPD-PNPKYINKLYVNHPDKFRQYGIWERYAELYPDKDLIYTVGVSDYTKD-----WFFAQVTRKKDDNTYQGT-TWQIKFKLD--SVNRSSTYKLRVALASA 3213
            T+  +  A AS   A L+V  DN  + +       T +  +G V+ +  +G D L  +  +     Y D  +  P+   T G+ D    V++G+         VIL D      ++T T               + + +  G +G +T++   +  +   F  NL E R  F+   + + ++  ++   +  PLP          +   +A    N    +       E   KY +S   +D+ VHG       S     G WL+  + +   GGP+  +LT  V        +S H+   +  P    G  + + FGP +   N     G    L D       +    W   F +S       +    +RG V G++ + +        ++  G Y              E  ++Q+WA    DG F + ++  G Y +  +  G  GD+ +D       G ++  G +      +E    G  +W +G PD+S+ EF   D P+P +     ++ P  F  +G ++ + + +P+  + YT+G SD   D     W     T    D  Y  T  W I F LD   + +  T  L + LA A
Sbjct:    5 TSLGVASACASVASAALKVTEDNSTITLANDRLTSTFAKDKGRVSELFLDGQDLLGPISGNTGVGPYLDC-YCIPSGFYTAGSTDPRLEVVQGTDSTGTKYAGVILND------TYTPTGQ----------QFQQYWFLRDGETGLHTFSRLAYYNETTPFLRNLQEFRTLFRPNTELWTHLTSSE--AQTAPLPSKEAI--ANEVVVQDATWRFNNTPNDAYYTQFSEYFTKYTFSNLWRDNSVHGLYADGTNSNGTTYGAWLVMNTKDTYYGGPLHSDLT--VDGIVYNYLVSNHHG--EGTPNITNG--FDRTFGPQYYLFNG--GKGSKSSLEDLRSEAETLADPGWNADFYDSIAKHVIGYAPSSKRGSVQGQVKLPKGSTRPIAILTVDGQYFQ--------DNSVEASSHQYWAEMGQDGTFQLDHVKEGKYRLTVFADGIFGDFVHD-------GVEVQAGKVTKVQETWEQESAGVEVWRLGTPDKSSGEFLHGDAPDPTH----PLHPPQHFIYWGAYD-WQQDFPN-GVNYTIGSSDPAVDFNTVHWSVYGPTPANPDVEYDTTHDWTINFSLDKKQLQQRKTATLTIQLAGA 558          

HSP 2 Score: 64.6994 bits (156), Expect = 4.705e-9
Identity = 53/192 (27.60%), Postives = 90/192 (46.88%), Query Frame = 1

Query: 1048 ADYQFWTRADENGNFTISNVRPGDYNLFAWVPGFVGDYNKFGDIMKITSGSYMELGQCIYKPPRDGPTLWEIGIPDRLAAEFYVPD-PNPQHINKLFINHPDRFRQYGLWDRYSELYPDADLVYTIGVSDYRKDW------FFAQVPRKRE---DNTHQGTTWQIKFEL--SGVIQGTTYTLRVALASATLA 1587
            + +Q+W    ++G F + +V+ G Y L  +  G  GD+    D +++ +G   ++ Q  ++    G  +W +G PD+ + EF   D P+P H     ++ P  F  +G +D + + +P+  + YTIG SD   D+       +   P   +   D TH    W I F L    + Q  T TL + LA A  A
Sbjct:  382 SSHQYWAEMGQDGTFQLDHVKEGKYRLTVFADGIFGDF--VHDGVEVQAGKVTKV-QETWEQESAGVEVWRLGTPDKSSGEFLHGDAPDPTHP----LHPPQHFIYWGAYD-WQQDFPNG-VNYTIGSSDPAVDFNTVHWSVYGPTPANPDVEYDTTHD---WTINFSLDKKQLQQRKTATLTIQLAGAKTA 561          

BLAST of Ca_04975 vs. SwissProt
Match: RGLB_ASPTN (Probable rhamnogalacturonate lyase B OS=Aspergillus terreus (strain NIH 2624 / FGSC A1156) GN=rglB PE=3 SV=1)

HSP 1 Score: 78.9518 bits (193), Expect = 2.411e-13
Identity = 107/465 (23.01%), Postives = 178/465 (38.28%), Query Frame = 1

Query: 1900 FIMLSGCSGFYTYAIYEHLEDWPAF--NLDETRIAFKLRKDKFHYMAMADNRQRNMPLPDDRVPPRGQALAYPEAVLLVNPIEPELK----GEVDDKYQYSCDNKDSQVHGWI-----SMDPAVGFWLITPSNEFRSGGPVKQNLTSHVGPTTLAVFLSAHYSGQDLVPKFKAGEAWKKVFGPVFIYLNSPYDGGDPIKLWDDAKLQMLMEVQSWPYSFPESD-----DFPKWDERGYVSGRLLIKERCIDDDDYVSAKGAYVGLAPPGDVGSWQRECKNYQFWATADYDGYFSISNIHVGDYNVYAWVPGYIGDYKYDVVISITEGCDIDIGDLVYEPLRDGPTLWEIGIPDRSAAEF---YVPDPNPKYINKLYVNHPDKFRQYGIWERYAELYPDKDLIYTVGVSDYTKD-----WFFAQVTRKKDDNTYQGT-TWQIKFKL--DSVNRSSTYKLRVALASA 3213
            + +  G +G + ++   +  +   F  NL E R  F+   D + ++  ++   +  PLP D     G+ +   +A    N    +       E   KY +S   +D+ VHG       S     G WL+  + +   GGP+  +LT  V        +S H+   +  P    G  + + FGP F   N    GG    L +       +   SW   F +S       +    +RG V G++ +          ++  G Y              + ++YQ+W   D +G F + ++  G Y +  +  G  GDY  D V  +       I D  ++P   G  +W +G PD+S+ EF      DP        +  HP ++  Y     + + +P+  + YT+G SD   D     W     T    D  Y  T  W I F L    + +  T  L + LA A
Sbjct:  123 WFLRDGETGLHMFSRLAYYNETTPFLRNLQEFRTLFRPNTDLWTHLTSSE--LQTAPLPSDEAV--GKQVVVQDATWRFNNTPNDAYYTQFSEYFTKYTFSNAWRDNNVHGLYADGSTSNGTTFGAWLVMNTKDTYYGGPLHSDLT--VDGIVYNYIVSNHHG--EGTPNITNG--FDRTFGPQFYLFN----GGGSSSLNELRSEAESLADPSWNVEFYDSIAKHVVGYVPSSKRGSVQGQIKLPRGATRPIAILTVDGQYFQ--------DNSVDPRSYQYWVEMDANGKFQLDHVVEGKYRLTVYADGIFGDYVRDGV-QVRGRKTTRIND-SWQPESAGVEVWRLGTPDKSSGEFLHGVARDPT-------HPLHPPEYLIYWGAYDWQQDFPN-GVNYTIGSSDPATDFNTVHWSVFGPTPDNPDVEYDTTHDWAINFSLTKKQLQKRKTATLTIQLAGA 555          

HSP 2 Score: 62.003 bits (149), Expect = 3.049e-8
Identity = 53/190 (27.89%), Postives = 86/190 (45.26%), Query Frame = 1

Query: 1054 YQFWTRADENGNFTISNVRPGDYNLFAWVPGFVGDYNKFGDIMKITSGSYMELGQCIYKPPRDGPTLWEIGIPDRLAAEF-YVPDPNPQHINKLFINHPDRFRQYGLWDRYSELYPDADLVYTIGVSDYRKDW------FFAQVPRKRE---DNTHQGTTWQIKFELS--GVIQGTTYTLRVALASATLA 1587
            YQ+W   D NG F + +V  G Y L  +  G  GDY + G  +++       +    ++P   G  +W +G PD+ + EF +    +P H     ++ P+    +G +D + + +P+  + YTIG SD   D+       F   P   +   D TH    W I F L+   + +  T TL + LA A  A
Sbjct:  381 YQYWVEMDANGKFQLDHVVEGKYRLTVYADGIFGDYVRDG--VQVRGRKTTRINDS-WQPESAGVEVWRLGTPDKSSGEFLHGVARDPTHP----LHPPEYLIYWGAYD-WQQDFPNG-VNYTIGSSDPATDFNTVHWSVFGPTPDNPDVEYDTTHD---WAINFSLTKKQLQKRKTATLTIQLAGAKTA 558          

BLAST of Ca_04975 vs. SwissProt
Match: RGLB_ASPFU (Probable rhamnogalacturonate lyase B OS=Neosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100) GN=rglB PE=3 SV=1)

HSP 1 Score: 67.781 bits (164), Expect = 5.557e-10
Identity = 89/388 (22.94%), Postives = 146/388 (37.63%), Query Frame = 1

Query: 2170 KYQYSCDNKDSQVHGWI-----SMDPAVGFWLITPSNEFRSGGPVKQNLTSHVGPTTLAVFLSAHYSGQDLVPKFKAGEAWKKVFGPVFIYLNSPYDGGDPIKLWDDAKLQMLMEVQSWPYSFPESD-----DFPKWDERGYVSGRLLIKERCIDDDDYVSAKGAYV---GLAPPGDVGSWQRECKNYQFWATADYDGYFSISNIHVGDYNVYAWVPGYIGDYKYDVVISITEGCDIDIGDLVYEPLRDGPTLWEIGIPDRSAAEF---YVPDPNPKYINKLYVNHPDKFRQY-GIWERYAELYPDKDLIYTVGVSDYTKD-----WFFAQVTRKKDDNTYQGT-TWQIKFKLDSVNRSSTYKLRVALASATYSELQVRVNDPKANRP 3264
            KY +S   +D+ VHG       S     G WL+       + GP+  +LT  V        +S H+   +  P    G  + + FGP F   N     G    L D       +   SW   F +S       +    +RG V GR+ + +   +    ++  G Y     + P            +YQ+W   D  G F I  +  G Y +  +  G  GD+  D V ++  G    + +  ++    G  +W +G PD+S+ EF      DP        +  HP ++  Y G ++  ++    K + YT+G SD   D     W     T    +  Y  T  W+I F L      ++ K  + +  A         ++ KA+ P
Sbjct:  216 KYTFSNQWRDNDVHGLYGDGTNSNGSTYGAWLV-----MNTKGPLHSDLT--VDGIVYNYIVSNHHG--EGTPNITNG--FDRTFGPQFYLFNG--GKGSTSSLQDLRSEAAKLADPSWNAEFYDSIAKHVVGYVPSSKRGSVDGRIKLPKGASNPIAILTVDGQYFQDNSVVP-----------SSYQYWTDIDTSGRFRIDRVVEGKYRLTVYADGIFGDFVRDGV-TVRAGKTTTVKE-KWDAESAGKEIWRLGTPDKSSGEFRHGVARDPT-------HPLHPPEYLIYWGAYDWQSDF--PKGIDYTIGSSDPATDFNTVHWSVFGPTPDNPNVEYNTTHDWKINFSLTKKQLRNSKKATLTIQLAGAKTASGNTDEYKASEP 568          

HSP 2 Score: 58.5362 bits (140), Expect = 3.372e-7
Identity = 52/189 (27.51%), Postives = 82/189 (43.39%), Query Frame = 1

Query: 1048 ADYQFWTRADENGNFTISNVRPGDYNLFAWVPGFVGDYNKFGDIMKITSGSYMELGQCIYKPPRDGPTLWEIGIPDRLAAEF-YVPDPNPQHINKLFINHPDRFRQYGLWDRYSELYPDADLVYTIGVSDYRKDW----FFAQVPRKREDNTHQGTT--WQIKFELS--GVIQGTTYTLRVALASATLA 1587
            + YQ+WT  D +G F I  V  G Y L  +  G  GD+ + G  + + +G    + +  +     G  +W +G PD+ + EF +    +P H     ++ P+    +G +D  S+     D  YTIG SD   D+    +    P     N    TT  W+I F L+   +      TL + LA A  A
Sbjct:  377 SSYQYWTDIDTSGRFRIDRVVEGKYRLTVYADGIFGDFVRDG--VTVRAGKTTTVKE-KWDAESAGKEIWRLGTPDKSSGEFRHGVARDPTHP----LHPPEYLIYWGAYDWQSDFPKGID--YTIGSSDPATDFNTVHWSVFGPTPDNPNVEYNTTHDWKINFSLTKKQLRNSKKATLTIQLAGAKTA 556          

BLAST of Ca_04975 vs. SwissProt
Match: RGLB_ASPFC (Probable rhamnogalacturonate lyase B OS=Neosartorya fumigata (strain CEA10 / CBS 144.89 / FGSC A1163) GN=rglB PE=3 SV=1)

HSP 1 Score: 67.781 bits (164), Expect = 5.557e-10
Identity = 89/388 (22.94%), Postives = 146/388 (37.63%), Query Frame = 1

Query: 2170 KYQYSCDNKDSQVHGWI-----SMDPAVGFWLITPSNEFRSGGPVKQNLTSHVGPTTLAVFLSAHYSGQDLVPKFKAGEAWKKVFGPVFIYLNSPYDGGDPIKLWDDAKLQMLMEVQSWPYSFPESD-----DFPKWDERGYVSGRLLIKERCIDDDDYVSAKGAYV---GLAPPGDVGSWQRECKNYQFWATADYDGYFSISNIHVGDYNVYAWVPGYIGDYKYDVVISITEGCDIDIGDLVYEPLRDGPTLWEIGIPDRSAAEF---YVPDPNPKYINKLYVNHPDKFRQY-GIWERYAELYPDKDLIYTVGVSDYTKD-----WFFAQVTRKKDDNTYQGT-TWQIKFKLDSVNRSSTYKLRVALASATYSELQVRVNDPKANRP 3264
            KY +S   +D+ VHG       S     G WL+       + GP+  +LT  V        +S H+   +  P    G  + + FGP F   N     G    L D       +   SW   F +S       +    +RG V GR+ + +   +    ++  G Y     + P            +YQ+W   D  G F I  +  G Y +  +  G  GD+  D V ++  G    + +  ++    G  +W +G PD+S+ EF      DP        +  HP ++  Y G ++  ++    K + YT+G SD   D     W     T    +  Y  T  W+I F L      ++ K  + +  A         ++ KA+ P
Sbjct:  216 KYTFSNQWRDNDVHGLYGDGTNSNGSTYGAWLV-----MNTKGPLHSDLT--VDGIVYNYIVSNHHG--EGTPNITNG--FDRTFGPQFYLFNG--GKGSTSSLQDLRSEAAKLADPSWNAEFYDSIAKHVVGYVPSSKRGSVDGRIKLPKGASNPIAILTVDGQYFQDNSVVP-----------SSYQYWTDIDTSGRFRIDRVVEGKYRLTVYADGIFGDFVRDGV-TVRAGKTTTVKE-KWDAESAGKEIWRLGTPDKSSGEFRHGVARDPT-------HPLHPPEYLIYWGAYDWQSDF--PKGIDYTIGSSDPATDFNTVHWSVFGPTPDNPNVEYNTTHDWKINFSLTKKQLRNSKKATLTIQLAGAKTASGNTDEYKASEP 568          

HSP 2 Score: 58.5362 bits (140), Expect = 3.372e-7
Identity = 52/189 (27.51%), Postives = 82/189 (43.39%), Query Frame = 1

Query: 1048 ADYQFWTRADENGNFTISNVRPGDYNLFAWVPGFVGDYNKFGDIMKITSGSYMELGQCIYKPPRDGPTLWEIGIPDRLAAEF-YVPDPNPQHINKLFINHPDRFRQYGLWDRYSELYPDADLVYTIGVSDYRKDW----FFAQVPRKREDNTHQGTT--WQIKFELS--GVIQGTTYTLRVALASATLA 1587
            + YQ+WT  D +G F I  V  G Y L  +  G  GD+ + G  + + +G    + +  +     G  +W +G PD+ + EF +    +P H     ++ P+    +G +D  S+     D  YTIG SD   D+    +    P     N    TT  W+I F L+   +      TL + LA A  A
Sbjct:  377 SSYQYWTDIDTSGRFRIDRVVEGKYRLTVYADGIFGDFVRDG--VTVRAGKTTTVKE-KWDAESAGKEIWRLGTPDKSSGEFRHGVARDPTHP----LHPPEYLIYWGAYDWQSDFPKGID--YTIGSSDPATDFNTVHWSVFGPTPDNPNVEYNTTHDWKINFSLTKKQLRNSKKATLTIQLAGAKTA 556          

BLAST of Ca_04975 vs. SwissProt
Match: RGLB_NEOFI (Probable rhamnogalacturonate lyase B OS=Neosartorya fischeri (strain ATCC 1020 / DSM 3700 / FGSC A1164 / NRRL 181) GN=rglB PE=3 SV=1)

HSP 1 Score: 66.6254 bits (161), Expect = 1.238e-9
Identity = 90/373 (24.13%), Postives = 141/373 (37.80%), Query Frame = 1

Query: 2170 KYQYSCDNKDSQVHGWI-----SMDPAVGFWLITPSNEFRSGGPVKQNLTSHVGPTTLAVFLSAHYSGQDLVPKFKAGEAWKKVFGPVFIYLNSPYDGGDPIKLWDDAKLQMLMEVQSWPYSFPESD-----DFPKWDERGYVSGRLLIKERCIDDDDYVSAKGAYV---GLAPPGDVGSWQRECKNYQFWATADYDGYFSISNIHVGDYNVYAWVPGYIGDYKYDVVISITEGCDIDIGDLVYEPLRDGPTLWEIGIPDRSAAEF---YVPDPNPKYINKLYVNHPDKFRQY-GIWERYAELYPDKDLIYTVGVSDYTKD-----WFFAQVTRKKDDNTYQGT-TWQIKFKL--DSVNRSSTYKLRVALASA 3213
            KY +S   +D+ VHG       S     G WL+       + GP+  +LT  V        +S H+   +  P    G  + + FGP F   N     G    L D       +   SW   F +S       +    +RG V GR+ + +   +    ++  G Y     + P            +YQ+W   D  G F I  +  G Y +  +  G  GD+  D V ++  G    + +  ++    G  +W +G PD+S+ EF      DP        +  HP ++  Y G ++  ++L   K + Y +G SD   D     W     T    D  Y  T  W+I F L    +  S    L + LA A
Sbjct:  216 KYTFSNQWRDNDVHGLYGDGTNSNGSTYGAWLV-----MNTKGPLHSDLT--VDGIVYNYIVSNHHG--EGTPNITNG--FDRTFGPQFYLFNG--GKGSTSSLQDLRSEAAKLADPSWNAEFYDSIAKHVVGYVPSSKRGSVDGRVKLPKGATNPIAILTVDGQYFQDNSVVP-----------SSYQYWTDIDTSGKFRIDRVVEGKYRLTVYADGIFGDFVRDGV-TVKAGKTTTVKE-KWDAESAGKEVWRLGTPDKSSGEFRHGVARDPT-------HPLHPPEYLIYWGAYDWQSDL--PKGIDYRIGSSDPATDFNTVHWSVFGPTPDNPDVEYNTTHDWKINFSLTKKQLRNSKKATLTIQLAGA 553          

HSP 2 Score: 57.7658 bits (138), Expect = 5.751e-7
Identity = 50/189 (26.46%), Postives = 80/189 (42.33%), Query Frame = 1

Query: 1048 ADYQFWTRADENGNFTISNVRPGDYNLFAWVPGFVGDYNKFGDIMKITSGSYMELGQCIYKPPRDGPTLWEIGIPDRLAAEF-YVPDPNPQHINKLFINHPDRFRQYGLWDRYSELYPDADLVYTIGVSDYRKDW------FFAQVPRKREDNTHQGTTWQIKFELS--GVIQGTTYTLRVALASATLA 1587
            + YQ+WT  D +G F I  V  G Y L  +  G  GD+ + G  +K    + ++     +     G  +W +G PD+ + EF +    +P H     ++ P+    +G +D  S+L    D  Y IG SD   D+       F   P   +   +    W+I F L+   +      TL + LA A  A
Sbjct:  377 SSYQYWTDIDTSGKFRIDRVVEGKYRLTVYADGIFGDFVRDGVTVKAGKTTTVKEK---WDAESAGKEVWRLGTPDKSSGEFRHGVARDPTHP----LHPPEYLIYWGAYDWQSDLPKGID--YRIGSSDPATDFNTVHWSVFGPTPDNPDVEYNTTHDWKINFSLTKKQLRNSKKATLTIQLAGAKTA 556          

BLAST of Ca_04975 vs. SwissProt
Match: TRAD1_BOVIN (TRAF-type zinc finger domain-containing protein 1 OS=Bos taurus GN=TRAFD1 PE=2 SV=1)

HSP 1 Score: 61.2326 bits (147), Expect = 5.202e-8
Identity = 28/83 (33.73%), Postives = 40/83 (48.19%), Query Frame = 1

Query:   10 VSDQPTSICIHCDRPIPAANIDLHSVHCARNLEKCKVCGDMVPKQHAEDHYLSTHAPEVCGNRTELCQLCNKYVRLRERYNHE 258
            ++DQ T +C +C + IP  N  +H +HC RN+  C VC +  PK   E H  + H    C         CNK +  R+   HE
Sbjct:    5 LNDQDTRLCDNCKKEIPVFNFTIHEIHCQRNIGVCPVCKEPFPKCDMETHMATEHCQVTC--------KCNKKLEKRQLKKHE 79          

BLAST of Ca_04975 vs. SwissProt
Match: TRAD1_MACFA (TRAF-type zinc finger domain-containing protein 1 OS=Macaca fascicularis GN=TRAFD1 PE=2 SV=1)

HSP 1 Score: 60.077 bits (144), Expect = 1.159e-7
Identity = 27/83 (32.53%), Postives = 38/83 (45.78%), Query Frame = 1

Query:   10 VSDQPTSICIHCDRPIPAANIDLHSVHCARNLEKCKVCGDMVPKQHAEDHYLSTHAPEVCGNRTELCQLCNKYVRLRERYNHE 258
            + DQ T +C +C + IP  N  +H +HC RN+  C +C +  PK   E H  + H    C         CNK +  R    HE
Sbjct:    5 LDDQETRLCDNCKKEIPVFNFTIHEIHCQRNIGMCPICKEPFPKSDMETHMAAEHCQVTC--------KCNKKLEKRLLKKHE 79          

BLAST of Ca_04975 vs. TrEMBL
Match: G7J4L9_MEDTR (Rhamnogalacturonate lyase OS=Medicago truncatula GN=MTR_3g111410 PE=4 SV=1)

HSP 1 Score: 1203.73 bits (3113), Expect = 0.000e+0
Identity = 577/635 (90.87%), Postives = 599/635 (94.33%), Query Frame = 1

Query: 1579 TLAELQVVMDNGILRVTLSNPEGIVTGIQYNGIDNLLEVLNDESNRGY---WDLVWSSPTSTGTTGNFDVIKGSTFKVILEDEDQVELSFTRTWDASMEGKLVPLNIDKRFIMLSGCSGFYTYAIYEHLEDWPAFNLDETRIAFKLRKDKFHYMAMADNRQRNMPLPDDRVPPRGQALAYPEAVLLVNPIEPELKGEVDDKYQYSCDNKDSQVHGWISMDPAVGFWLITPSNEFRSGGPVKQNLTSHVGPTTLAVFLSAHYSGQDLVPKFKAGEAWKKVFGPVFIYLNSPYDGGDPIKLWDDAKLQMLMEVQSWPYSFPESDDFPKWDERGYVSGRLLIKERCIDDDDYVSAKGAYVGLAPPGDVGSWQRECKNYQFWATADYDGYFSISNIHVGDYNVYAWVPGYIGDYKYDVVISITEGCDIDIGDLVYEPLRDGPTLWEIGIPDRSAAEFYVPDPNPKYINKLYVNHPDKFRQYGIWERYAELYPDKDLIYTVGVSDYTKDWFFAQVTRKKDDNTYQGTTWQIKFKLDSVNRSSTYKLRVALASATYSELQVRVNDPKANRPLFSSGLIGKDNSIARHGIHGLNWLYSVGISGNLLVEGDYNTIFLTQARGNGPFQAIMYDYIRLEGPAAIS 3474
            ++ +  VVMDNGILRVTLSNPEGIVTGIQY+ IDNLLEVLN+ESNRGY   WDLVWSSPTSTGTTG FDVIK +TFKVILEDEDQVELSFTRTWDAS+EGKLVPLNIDKRFIML GCSGFY+YAIYEHLEDWPAFNLDETRIAFKLRKDKFHYMAMADNRQRNMPLPDDRV PRGQALAYPEAVLLVNPIEPELKGEVDDKYQYSCDNKDSQVHGWI MDPAVGFWLITPSNEFRSGGPVKQNLTSHVGPTTLAVFLSAHYSG+DLVPKFKAGEAWKKVFGPVFIY+NSPYDG DPIKLWDDAKLQMLMEVQSWPY+FPESDDFPKWDERG V GRLL+KER I DDDY+SA  AYVGLA PG+VGSWQRECKNYQFWA AD DGYFSISNIHVGDYN YAWVPG+IGDYKYDVVISITEGCDIDIGDLVYEP RDGPTLWEIGIPDRSAAEFYVPDPNPKYINKLYVNHPDKFRQYGIWERYAELYPD DLIYT+GVSD+TKDWFFAQVTRKK+DNTYQGTTWQIKF LD+VNR  +YKLRVALASATYSELQVRVNDPK NRPLFSSGLIGKDNSIARHGIHGL WLY+V I G+LLVEGD NTIFLTQARGN PFQAIMYDYIRLEGPA  S
Sbjct:    9 SIQDHHVVMDNGILRVTLSNPEGIVTGIQYSDIDNLLEVLNEESNRGYTLYWDLVWSSPTSTGTTGKFDVIKATTFKVILEDEDQVELSFTRTWDASLEGKLVPLNIDKRFIMLRGCSGFYSYAIYEHLEDWPAFNLDETRIAFKLRKDKFHYMAMADNRQRNMPLPDDRVAPRGQALAYPEAVLLVNPIEPELKGEVDDKYQYSCDNKDSQVHGWICMDPAVGFWLITPSNEFRSGGPVKQNLTSHVGPTTLAVFLSAHYSGEDLVPKFKAGEAWKKVFGPVFIYVNSPYDGSDPIKLWDDAKLQMLMEVQSWPYNFPESDDFPKWDERGNVCGRLLVKERYI-DDDYLSANCAYVGLATPGEVGSWQRECKNYQFWAKADDDGYFSISNIHVGDYNFYAWVPGFIGDYKYDVVISITEGCDIDIGDLVYEPPRDGPTLWEIGIPDRSAAEFYVPDPNPKYINKLYVNHPDKFRQYGIWERYAELYPDNDLIYTIGVSDFTKDWFFAQVTRKKEDNTYQGTTWQIKFNLDNVNRKGSYKLRVALASATYSELQVRVNDPKTNRPLFSSGLIGKDNSIARHGIHGLYWLYNVNIPGSLLVEGDNNTIFLTQARGNIPFQAIMYDYIRLEGPATSS 642          

HSP 2 Score: 287.345 bits (734), Expect = 2.450e-74
Identity = 126/183 (68.85%), Postives = 157/183 (85.79%), Query Frame = 1

Query: 1051 DYQFWTRADENGNFTISNVRPGDYNLFAWVPGFVGDYNKFGDIMKITSGSYMELGQCIYKPPRDGPTLWEIGIPDRLAAEFYVPDPNPQHINKLFINHPDRFRQYGLWDRYSELYPDADLVYTIGVSDYRKDWFFAQVPRKREDNTHQGTTWQIKFELSGVIQGTTYTLRVALASATLAELQV 1599
            +YQFW +AD++G F+ISN+  GDYN +AWVPGF+GDY K+  ++ IT G  +++G  +Y+PPRDGPTLWEIGIPDR AAEFYVPDPNP++INKL++NHPD+FRQYG+W+RY+ELYPD DL+YTIGVSD+ KDWFFAQV RK+EDNT+QGTTWQIKF L  V +  +Y LRVALASAT +ELQV
Sbjct:  381 NYQFWAKADDDGYFSISNIHVGDYNFYAWVPGFIGDY-KYDVVISITEGCDIDIGDLVYEPPRDGPTLWEIGIPDRSAAEFYVPDPNPKYINKLYVNHPDKFRQYGIWERYAELYPDNDLIYTIGVSDFTKDWFFAQVTRKKEDNTYQGTTWQIKFNLDNVNRKGSYKLRVALASATYSELQV 562          

HSP 3 Score: 231.106 bits (588), Expect = 2.084e-57
Identity = 109/147 (74.15%), Postives = 123/147 (83.67%), Query Frame = 1

Query:  610 NRGFIMLRGSSGFYSYGIYEHLRGWPDFDLSETRITFKLRKDKFQYMAVADDRRRIMPFPEDRLPGRCQPLAYQEAVLLVNPKDPRLKGEVDDKYQYSCKNIDNRVHGWISFNPHVGFWQITPSDEFRSGGPLKQNLTSHVGPTTLA 1050
            ++ FIMLRG SGFYSY IYEHL  WP F+L ETRI FKLRKDKF YMA+AD+R+R MP P+DR+  R Q LAY EAVLLVNP +P LKGEVDDKYQYSC N D++VHGWI  +P VGFW ITPS+EFRSGGP+KQNLTSHVGPTTLA
Sbjct:  116 DKRFIMLRGCSGFYSYAIYEHLEDWPAFNLDETRIAFKLRKDKFHYMAMADNRQRNMPLPDDRVAPRGQALAYPEAVLLVNPIEPELKGEVDDKYQYSCDNKDSQVHGWICMDPAVGFWLITPSNEFRSGGPVKQNLTSHVGPTTLA 262          

HSP 4 Score: 69.707 bits (169), Expect = 8.029e-9
Identity = 33/52 (63.46%), Postives = 44/52 (84.62%), Query Frame = 1

Query:  472 LTLSPHSLVVMDNGIVQVTLSKPDGIVTGIKYNGVDNLLEVLNKEPNRGFIM 627
            L++  H  VVMDNGI++VTLS P+GIVTGI+Y+ +DNLLEVLN+E NRG+ +
Sbjct:    8 LSIQDHH-VVMDNGILRVTLSNPEGIVTGIQYSDIDNLLEVLNEESNRGYTL 58          

BLAST of Ca_04975 vs. TrEMBL
Match: I1K9M3_SOYBN (Uncharacterized protein OS=Glycine max PE=4 SV=1)

HSP 1 Score: 1186.02 bits (3067), Expect = 0.000e+0
Identity = 564/637 (88.54%), Postives = 602/637 (94.51%), Query Frame = 1

Query: 1579 TLAELQVVMDNGILRVTLSNPEGIVTGIQYNGIDNLLEVLNDESNRGYWDLVWSSPTSTGTTGNFDVIKGSTFKVILEDEDQVELSFTRTWDASMEGKLVPLNIDKRFIMLSGCSGFYTYAIYEHLEDWPAFNLDETRIAFKLRKDKFHYMAMADNRQRNMPLPDDRVPPRGQALAYPEAVLLVNPIEPELKGEVDDKYQYSCDNKDSQVHGWISMDPAVGFWLITPSNEFRSGGPVKQNLTSHVGPTTLAVFLSAHYSGQDLVPKFKAGEAWKKVFGPVFIYLNSPYDGGDPIKLWDDAKLQMLMEVQSWPYSFPESDDFPKWDERGYVSGRLLIKERCIDDDDYVSAKGAYVGLAPPGDVGSWQRECKNYQFWATADYDGYFSISNIHVGDYNVYAWVPGYIGDYKYDVVISITEGCDIDIGDLVYEPLRDGPTLWEIGIPDRSAAEFYVPDPNPKYINKLYVNHPDKFRQYGIWERYAELYPDKDLIYTVGVSDYTKDWFFAQVTRKKDDNTYQGTTWQIKFKLDSVNRSSTYKLRVALASATYSELQVRVNDPKANRPLFSSGLIGKDNSIARHGIHGLNWLYSVGISGNLLVEGDYNTIFLTQARGNGPFQAIMYDYIRLEGPAAISSSNKK 3489
            T+ +  VVM+NGIL+VTLSNP GIVTGIQYN IDNLLEVLNDESNRGYWDLVWSSPTSTGT+G FDVIKG+TFKV++E+EDQVELSFTRTWD S EGKLVPLNIDKRF+ML G SGFY+YAIYEHLE+WPAFNLDETRIAFKLRKDKFHYMAMADNRQRNMPLPDDR+PP+G+ALAYPEAVLLVNPIEPELKGEVDDKYQYSCDNKDSQVHGWI MDPAVGFWLITPSNEFRSGGP+KQNLTSHVGPTTLAVFLSAHYSG+DLVPKF +GEAWKKVFGPVFIYLNSPYDG DP+KLW+DAKLQM +EVQSWPYSFPES+DF KWD+RG VSGRLL++ER I +DDYVSAKGAYVGLAPPGDVGSWQRECKNYQFWA AD DGYFS+SNI  GDYNVYAWVPG+IGDYKYDVVI+ITEGCDID+GDLVYEP RDGPTLWEIGIPDRSAAEFYVPDPNPKYINKLYVNHPDKFRQYG+WERYAELYPDKDLIYT+GVSDYTKDWFFAQVTRKKDDNTYQGTTWQIKFKLDSVN+SS+YKLRVALASAT SELQVRVN+PKA RPLFSSGLIG+DNSIARHGIHGL WLY+VGI G  LVEGD NTIFLTQ RGNGPFQAIMYDYIRLE P + SS NKK
Sbjct:    9 TIQDNHVVMENGILKVTLSNPGGIVTGIQYNDIDNLLEVLNDESNRGYWDLVWSSPTSTGTSGTFDVIKGTTFKVVVENEDQVELSFTRTWDVSREGKLVPLNIDKRFVMLRGSSGFYSYAIYEHLEEWPAFNLDETRIAFKLRKDKFHYMAMADNRQRNMPLPDDRLPPKGKALAYPEAVLLVNPIEPELKGEVDDKYQYSCDNKDSQVHGWICMDPAVGFWLITPSNEFRSGGPLKQNLTSHVGPTTLAVFLSAHYSGEDLVPKFNSGEAWKKVFGPVFIYLNSPYDGTDPLKLWEDAKLQMSVEVQSWPYSFPESEDFAKWDDRGNVSGRLLVRERYI-NDDYVSAKGAYVGLAPPGDVGSWQRECKNYQFWARADDDGYFSVSNIRAGDYNVYAWVPGFIGDYKYDVVINITEGCDIDLGDLVYEPPRDGPTLWEIGIPDRSAAEFYVPDPNPKYINKLYVNHPDKFRQYGLWERYAELYPDKDLIYTIGVSDYTKDWFFAQVTRKKDDNTYQGTTWQIKFKLDSVNKSSSYKLRVALASATLSELQVRVNNPKAPRPLFSSGLIGRDNSIARHGIHGLYWLYNVGIPGTQLVEGD-NTIFLTQTRGNGPFQAIMYDYIRLEAPPS-SSFNKK 642          

HSP 2 Score: 299.286 bits (765), Expect = 6.231e-78
Identity = 131/187 (70.05%), Postives = 165/187 (88.24%), Query Frame = 1

Query: 1051 DYQFWTRADENGNFTISNVRPGDYNLFAWVPGFVGDYNKFGDIMKITSGSYMELGQCIYKPPRDGPTLWEIGIPDRLAAEFYVPDPNPQHINKLFINHPDRFRQYGLWDRYSELYPDADLVYTIGVSDYRKDWFFAQVPRKREDNTHQGTTWQIKFELSGVIQGTTYTLRVALASATLAELQVVMDN 1611
            +YQFW RAD++G F++SN+R GDYN++AWVPGF+GDY K+  ++ IT G  ++LG  +Y+PPRDGPTLWEIGIPDR AAEFYVPDPNP++INKL++NHPD+FRQYGLW+RY+ELYPD DL+YTIGVSDY KDWFFAQV RK++DNT+QGTTWQIKF+L  V + ++Y LRVALASATL+ELQV ++N
Sbjct:  378 NYQFWARADDDGYFSVSNIRAGDYNVYAWVPGFIGDY-KYDVVINITEGCDIDLGDLVYEPPRDGPTLWEIGIPDRSAAEFYVPDPNPKYINKLYVNHPDKFRQYGLWERYAELYPDKDLIYTIGVSDYTKDWFFAQVTRKKDDNTYQGTTWQIKFKLDSVNKSSSYKLRVALASATLSELQVRVNN 563          

HSP 3 Score: 243.047 bits (619), Expect = 5.299e-61
Identity = 134/245 (54.69%), Postives = 154/245 (62.86%), Query Frame = 1

Query:  496 VVMDNGIVQVTLSKPDGIVTGIKYNGVDNLLEVLNKEPNRGFIML-------RGSSGFY------------------------------------------------SYGIY-----EHLRGWPDFDLSETRITFKLRKDKFQYMAVADDRRRIMPFPEDRLPGRCQPLAYQEAVLLVNPKDPRLKGEVDDKYQYSCKNIDNRVHGWISFNPHVGFWQITPSDEFRSGGPLKQNLTSHVGPTTLA 1050
            VVM+NGI++VTLS P GIVTGI+YN +DNLLEVLN E NRG+  L        G+SG +                                                S G Y     EHL  WP F+L ETRI FKLRKDKF YMA+AD+R+R MP P+DRLP + + LAY EAVLLVNP +P LKGEVDDKYQYSC N D++VHGWI  +P VGFW ITPS+EFRSGGPLKQNLTSHVGPTTLA
Sbjct:   15 VVMENGILKVTLSNPGGIVTGIQYNDIDNLLEVLNDESNRGYWDLVWSSPTSTGTSGTFDVIKGTTFKVVVENEDQVELSFTRTWDVSREGKLVPLNIDKRFVMLRGSSGFYSYAIYEHLEEWPAFNLDETRIAFKLRKDKFHYMAMADNRQRNMPLPDDRLPPKGKALAYPEAVLLVNPIEPELKGEVDDKYQYSCDNKDSQVHGWICMDPAVGFWLITPSNEFRSGGPLKQNLTSHVGPTTLA 259          

BLAST of Ca_04975 vs. TrEMBL
Match: I1JV26_SOYBN (Uncharacterized protein OS=Glycine max PE=4 SV=1)

HSP 1 Score: 1180.24 bits (3052), Expect = 0.000e+0
Identity = 561/637 (88.07%), Postives = 601/637 (94.35%), Query Frame = 1

Query: 1579 TLAELQVVMDNGILRVTLSNPEGIVTGIQYNGIDNLLEVLNDESNRGYWDLVWSSPTSTGTTGNFDVIKGSTFKVILEDEDQVELSFTRTWDASMEGKLVPLNIDKRFIMLSGCSGFYTYAIYEHLEDWPAFNLDETRIAFKLRKDKFHYMAMADNRQRNMPLPDDRVPPRGQALAYPEAVLLVNPIEPELKGEVDDKYQYSCDNKDSQVHGWISMDPAVGFWLITPSNEFRSGGPVKQNLTSHVGPTTLAVFLSAHYSGQDLVPKFKAGEAWKKVFGPVFIYLNSPYDGGDPIKLWDDAKLQMLMEVQSWPYSFPESDDFPKWDERGYVSGRLLIKERCIDDDDYVSAKGAYVGLAPPGDVGSWQRECKNYQFWATADYDGYFSISNIHVGDYNVYAWVPGYIGDYKYDVVISITEGCDIDIGDLVYEPLRDGPTLWEIGIPDRSAAEFYVPDPNPKYINKLYVNHPDKFRQYGIWERYAELYPDKDLIYTVGVSDYTKDWFFAQVTRKKDDNTYQGTTWQIKFKLDSVNRSSTYKLRVALASATYSELQVRVNDPKANRPLFSSGLIGKDNSIARHGIHGLNWLYSVGISGNLLVEGDYNTIFLTQARGNGPFQAIMYDYIRLEGPAAISSSNKK 3489
            T+ +  VVM+NG+L+VTLSNPEGIVTGIQYN IDNLLEVLNDESNRGYWDLVWSSPTSTGT+G FDVIKG+TFKV++E+EDQVELSFTRTWD S+EGKLVPLNIDKRF+ML G SGFY+YAIYEHLEDWPAFNLDETRIAFKLRKDKFHYMAMADNRQRNMPLPDDR+PP+G+ALAYPEAVLLVNPIEPELKGEVDDKYQYSCDNKDSQVHGWI MDPAVGFWLITPSNEFRSGGP+KQNLTSHVGPTTLAVFLSAHYSG+DLVPKF AGEAWKKVFGPVFIYLNSPYDG  P+KLW+DAKLQML+EVQSWPYSFPES++F KWDERG VSGRLL++ER I +DDYVSAKGAYVGLAPPGDVGSWQRECKNYQFWA AD DGYFSISNI  GDYNV+AWVPG+IGDYKYD+V+ ITEGCDID+GDLVYEP RDGPTLWEIGIPDRSAAEFYVPDPNPKYINKLY NHPDKFRQYG+WERYAELYPDKDLIYTVGVSDYTKDWFFAQVTRKKDDNTYQGTTWQIKFKLD+VN+SS+YKLRVALASA  SELQVRVN+PKA RPLFSSGLIG+DNSIARHGIHGL WLY+V + G  LVEGD NTIFLTQ RGNGPFQAIMYDYIRLEGP + SS NKK
Sbjct:    9 TIQDNHVVMENGMLKVTLSNPEGIVTGIQYNDIDNLLEVLNDESNRGYWDLVWSSPTSTGTSGTFDVIKGTTFKVMVENEDQVELSFTRTWDVSLEGKLVPLNIDKRFVMLRGSSGFYSYAIYEHLEDWPAFNLDETRIAFKLRKDKFHYMAMADNRQRNMPLPDDRLPPKGKALAYPEAVLLVNPIEPELKGEVDDKYQYSCDNKDSQVHGWICMDPAVGFWLITPSNEFRSGGPLKQNLTSHVGPTTLAVFLSAHYSGEDLVPKFYAGEAWKKVFGPVFIYLNSPYDGSHPLKLWEDAKLQMLVEVQSWPYSFPESENFAKWDERGNVSGRLLVRERYI-NDDYVSAKGAYVGLAPPGDVGSWQRECKNYQFWAKADDDGYFSISNIRAGDYNVFAWVPGFIGDYKYDIVMKITEGCDIDLGDLVYEPPRDGPTLWEIGIPDRSAAEFYVPDPNPKYINKLYFNHPDKFRQYGLWERYAELYPDKDLIYTVGVSDYTKDWFFAQVTRKKDDNTYQGTTWQIKFKLDNVNKSSSYKLRVALASAALSELQVRVNNPKAPRPLFSSGLIGRDNSIARHGIHGLYWLYNVDMPGTQLVEGD-NTIFLTQTRGNGPFQAIMYDYIRLEGPPS-SSFNKK 642          

HSP 2 Score: 299.671 bits (766), Expect = 4.771e-78
Identity = 132/187 (70.59%), Postives = 164/187 (87.70%), Query Frame = 1

Query: 1051 DYQFWTRADENGNFTISNVRPGDYNLFAWVPGFVGDYNKFGDIMKITSGSYMELGQCIYKPPRDGPTLWEIGIPDRLAAEFYVPDPNPQHINKLFINHPDRFRQYGLWDRYSELYPDADLVYTIGVSDYRKDWFFAQVPRKREDNTHQGTTWQIKFELSGVIQGTTYTLRVALASATLAELQVVMDN 1611
            +YQFW +AD++G F+ISN+R GDYN+FAWVPGF+GDY K+  +MKIT G  ++LG  +Y+PPRDGPTLWEIGIPDR AAEFYVPDPNP++INKL+ NHPD+FRQYGLW+RY+ELYPD DL+YT+GVSDY KDWFFAQV RK++DNT+QGTTWQIKF+L  V + ++Y LRVALASA L+ELQV ++N
Sbjct:  378 NYQFWAKADDDGYFSISNIRAGDYNVFAWVPGFIGDY-KYDIVMKITEGCDIDLGDLVYEPPRDGPTLWEIGIPDRSAAEFYVPDPNPKYINKLYFNHPDKFRQYGLWERYAELYPDKDLIYTVGVSDYTKDWFFAQVTRKKDDNTYQGTTWQIKFKLDNVNKSSSYKLRVALASAALSELQVRVNN 563          

HSP 3 Score: 243.432 bits (620), Expect = 4.057e-61
Identity = 133/245 (54.29%), Postives = 155/245 (63.27%), Query Frame = 1

Query:  496 VVMDNGIVQVTLSKPDGIVTGIKYNGVDNLLEVLNKEPNRGFIML-------RGSSGFY------------------------------------------------SYGIY-----EHLRGWPDFDLSETRITFKLRKDKFQYMAVADDRRRIMPFPEDRLPGRCQPLAYQEAVLLVNPKDPRLKGEVDDKYQYSCKNIDNRVHGWISFNPHVGFWQITPSDEFRSGGPLKQNLTSHVGPTTLA 1050
            VVM+NG+++VTLS P+GIVTGI+YN +DNLLEVLN E NRG+  L        G+SG +                                                S G Y     EHL  WP F+L ETRI FKLRKDKF YMA+AD+R+R MP P+DRLP + + LAY EAVLLVNP +P LKGEVDDKYQYSC N D++VHGWI  +P VGFW ITPS+EFRSGGPLKQNLTSHVGPTTLA
Sbjct:   15 VVMENGMLKVTLSNPEGIVTGIQYNDIDNLLEVLNDESNRGYWDLVWSSPTSTGTSGTFDVIKGTTFKVMVENEDQVELSFTRTWDVSLEGKLVPLNIDKRFVMLRGSSGFYSYAIYEHLEDWPAFNLDETRIAFKLRKDKFHYMAMADNRQRNMPLPDDRLPPKGKALAYPEAVLLVNPIEPELKGEVDDKYQYSCDNKDSQVHGWICMDPAVGFWLITPSNEFRSGGPLKQNLTSHVGPTTLA 259          

BLAST of Ca_04975 vs. TrEMBL
Match: B9SMY4_RICCO (Lyase, putative OS=Ricinus communis GN=RCOM_0482630 PE=4 SV=1)

HSP 1 Score: 1042.34 bits (2694), Expect = 0.000e+0
Identity = 493/632 (78.01%), Postives = 562/632 (88.92%), Query Frame = 1

Query: 1597 VVMDNGILRVTLSNPEGIVTGIQYNGIDNLLEVLNDESNRGYWDLVWSSPTSTGTTGNFDVIKGSTFKVILEDEDQVELSFTRTWDASMEGKLVPLNIDKRFIMLSGCSGFYTYAIYEHLEDWPAFNLDETRIAFKLRKDKFHYMAMADNRQRNMPLPDDRVPPRGQALAYPEAVLLVNPIEPELKGEVDDKYQYSCDNKDSQVHGWISMDPAVGFWLITPSNEFRSGGPVKQNLTSHVGPTTLAVFLSAHYSGQDLVPKFKAGEAWKKVFGPVFIYLNSPYDGGDPIKLWDDAKLQMLMEVQSWPYSFPESDDFPKWDERGYVSGRLLIKERCIDDDDYVSAKGAYVGLAPPGDVGSWQRECKNYQFWATADYDGYFSISNIHVGDYNVYAWVPGYIGDYKYDVVISITEGCDIDIGDLVYEPLRDGPTLWEIGIPDRSAAEFYVPDPNPKYINKLYVNHPDKFRQYGIWERYAELYPDKDLIYTVGVSDYTKDWFFAQVTRKKDDNTYQGTTWQIKFKLDSVNRSSTYKLRVALASATYSELQVRVNDPKANRPLFSSGLIGKDNSIARHGIHGLNWLYSVGISGNLLVEGDYNTIFLTQARGNGPFQAIMYDYIRLEGPAAISSSNKKI 3492
            V++DNGIL+VTLSNPEGIVTGIQYNGI NLLEVLNDESNRGYWDLVWS+P STGTTG FDVIKG++FKVI+E E+QVELSFTR+WD S+EGKL+PLNIDKRF+ML G SGFY+YAI+EHL++WP FNL E RIAFKLRK+KFHYMA+ADNRQR MPLPDDR+ PRGQALAYPEAVLLVNP+E E KGE+DDKYQYSC+NKD +VHGWI MDP+VGFW ITPSNEFRSGGPVKQNLTSHVGPTTL+VFLSAHYSG+DLVPKF AGEAWKKVFGPVF+YLNS   G DP+ LW+DAK Q  +EVQSWPY FP S+D+PK ++RG VSGRLL+K+R +  DDY+SA GAYVGLAP GDVGSWQRECK+YQFW+ AD +GYFSI++I  GDYN+YAWVPG+IGDY+ DV I+IT GCDI++ DLVYEP RDGPTLWEIGIPDRSAAEFY+PDPNP YINKLYVNHPD+FRQYG+W+RYAELYPD DL+YTVGVSDY KDWFFAQV RKKDDNTYQ TTWQIKFKLD+V+++  YKLRVA+ASAT +ELQVR+NDPKAN  +FSSG+IGKDNSIARHGIHGL WLY+V + G  LV+G  NT+FLTQ R + PFQ IMYDYIRLE P A S+ NK I
Sbjct:   15 VMLDNGILQVTLSNPEGIVTGIQYNGIGNLLEVLNDESNRGYWDLVWSTPGSTGTTGIFDVIKGTSFKVIVETEEQVELSFTRSWDPSLEGKLIPLNIDKRFVMLRGSSGFYSYAIFEHLKEWPGFNLAEARIAFKLRKEKFHYMAVADNRQRYMPLPDDRLSPRGQALAYPEAVLLVNPVESEFKGELDDKYQYSCENKDIRVHGWICMDPSVGFWQITPSNEFRSGGPVKQNLTSHVGPTTLSVFLSAHYSGEDLVPKFVAGEAWKKVFGPVFMYLNSVLVGDDPLSLWEDAKEQTEIEVQSWPYFFPASEDYPKSEQRGSVSGRLLVKDRFV-SDDYISANGAYVGLAPQGDVGSWQRECKDYQFWSKADENGYFSINSIRTGDYNLYAWVPGFIGDYRCDVAITITSGCDINMHDLVYEPPRDGPTLWEIGIPDRSAAEFYIPDPNPMYINKLYVNHPDRFRQYGLWDRYAELYPDGDLVYTVGVSDYRKDWFFAQVNRKKDDNTYQRTTWQIKFKLDNVDKNGIYKLRVAIASATVAELQVRINDPKAN-IIFSSGMIGKDNSIARHGIHGLYWLYNVDVPGVRLVQGG-NTVFLTQPRSSSPFQGIMYDYIRLEEPPA-SAPNKII 642          

HSP 2 Score: 291.197 bits (744), Expect = 1.697e-75
Identity = 137/218 (62.84%), Postives = 171/218 (78.44%), Query Frame = 1

Query: 1051 DYQFWTRADENGNFTISNVRPGDYNLFAWVPGFVGDYNKFGDIMKITSGSYMELGQCIYKPPRDGPTLWEIGIPDRLAAEFYVPDPNPQHINKLFINHPDRFRQYGLWDRYSELYPDADLVYTIGVSDYRKDWFFAQVPRKREDNTHQGTTWQIKFELSGVIQGTTYTLRVALASATLAELQVVMDNGILRVTLSNPE-GIVTGIQYNGIDNLLEVLN 1701
            DYQFW++ADENG F+I+++R GDYNL+AWVPGF+GDY +    + ITSG  + +   +Y+PPRDGPTLWEIGIPDR AAEFY+PDPNP +INKL++NHPDRFRQYGLWDRY+ELYPD DLVYT+GVSDYRKDWFFAQV RK++DNT+Q TTWQIKF+L  V +   Y LRVA+ASAT+AELQV +++    +  S+   G    I  +GI  L  + N
Sbjct:  378 DYQFWSKADENGYFSINSIRTGDYNLYAWVPGFIGDY-RCDVAITITSGCDINMHDLVYEPPRDGPTLWEIGIPDRSAAEFYIPDPNPMYINKLYVNHPDRFRQYGLWDRYAELYPDGDLVYTVGVSDYRKDWFFAQVNRKKDDNTYQRTTWQIKFKLDNVDKNGIYKLRVAIASATVAELQVRINDPKANIIFSSGMIGKDNSIARHGIHGLYWLYN 594          

HSP 3 Score: 240.35 bits (612), Expect = 3.435e-60
Identity = 130/245 (53.06%), Postives = 154/245 (62.86%), Query Frame = 1

Query:  496 VVMDNGIVQVTLSKPDGIVTGIKYNGVDNLLEVLNKEPNRGFIML-------RGSSGFY------------------------------------------------SYGIY-----EHLRGWPDFDLSETRITFKLRKDKFQYMAVADDRRRIMPFPEDRLPGRCQPLAYQEAVLLVNPKDPRLKGEVDDKYQYSCKNIDNRVHGWISFNPHVGFWQITPSDEFRSGGPLKQNLTSHVGPTTLA 1050
            V++DNGI+QVTLS P+GIVTGI+YNG+ NLLEVLN E NRG+  L        G++G +                                                S G Y     EHL+ WP F+L+E RI FKLRK+KF YMAVAD+R+R MP P+DRL  R Q LAY EAVLLVNP +   KGE+DDKYQYSC+N D RVHGWI  +P VGFWQITPS+EFRSGGP+KQNLTSHVGPTTL+
Sbjct:   15 VMLDNGILQVTLSNPEGIVTGIQYNGIGNLLEVLNDESNRGYWDLVWSTPGSTGTTGIFDVIKGTSFKVIVETEEQVELSFTRSWDPSLEGKLIPLNIDKRFVMLRGSSGFYSYAIFEHLKEWPGFNLAEARIAFKLRKEKFHYMAVADNRQRYMPLPDDRLSPRGQALAYPEAVLLVNPVESEFKGELDDKYQYSCENKDIRVHGWICMDPSVGFWQITPSNEFRSGGPVKQNLTSHVGPTTLS 259          

BLAST of Ca_04975 vs. TrEMBL
Match: M5XJZ7_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa026307mg PE=4 SV=1)

HSP 1 Score: 1041.18 bits (2691), Expect = 0.000e+0
Identity = 486/635 (76.54%), Postives = 559/635 (88.03%), Query Frame = 1

Query: 1582 LAELQVVMDNGILRVTLSNPEGIVTGIQYNGIDNLLEVLNDESNRGYWDLVWSSPTSTGTTGNFDVIKGSTFKVILEDEDQVELSFTRTWDASMEGKLVPLNIDKRFIMLSGCSGFYTYAIYEHLEDWPAFNLDETRIAFKLRKDKFHYMAMADNRQRNMPLPDDRVPPRGQALAYPEAVLLVNPIEPELKGEVDDKYQYSCDNKDSQVHGWISMDPAVGFWLITPSNEFRSGGPVKQNLTSHVGPTTLAVFLSAHYSGQDLVPKFKAGEAWKKVFGPVFIYLNSPYDGGDPIKLWDDAKLQMLMEVQSWPYSFPESDDFPKWDERGYVSGRLLIKERCIDDDDYVSAKGAYVGLAPPGDVGSWQRECKNYQFWATADYDGYFSISNIHVGDYNVYAWVPGYIGDYKYDVVISITEGCDIDIGDLVYEPLRDGPTLWEIGIPDRSAAEFYVPDPNPKYINKLYVNHPDKFRQYGIWERYAELYPDKDLIYTVGVSDYTKDWFFAQVTRKKDDNTYQGTTWQIKFKLDSVNRSSTYKLRVALASATYSELQVRVNDPKANRPLFSSGLIGKDNSIARHGIHGLNWLYSVGISGNLLVEGDYNTIFLTQARGNGPFQAIMYDYIRLEGPAAISSSNK 3486
            + +  V+MDNGI++VTLSNP+GIVTGI++NGIDNLLE+ N+E NRGYWDLVWSS  STGTTG FDVIKG++F+VI+E E+QVELSFTR WD S +GKLVPLNIDKRFIML G SGFY+YAIYEHL +WP F L ETRIAFKLRKDKFHYMAMADNRQRNMPLPDDR+P RGQALA+PEAVLLVNP+EPE KGEVDDKYQYSCDNKD++VHGWI  +P VGFW ITPSNEFRSGGP KQNLTSHVGPTTLA+FLSAHYSG+DLVPKF AGE WKKVFGPVF+Y NS Y+G DP+ LW+DAK QM+MEVQ WPYSFP S+DFPK  +RG VSGRLLI++R I  DDY+ A+GAYVGLAP GDVGSWQRECKNYQFW TAD +GYF I  I  GDYN+ A VPG+IGDY+Y+VV++IT GC++++GDLVYEP R+GPTLWEIGIPDRSAAEFYVPDPNPKYINKL+V+HPD+FRQYG+WERY++LYP+KDL+YTVGVSDY+KDWFFAQV RKKDDN YQGTTWQIKF LD+V++S  YKLR+A+ASAT SELQVRVNDPKA  PLFSSG+IGKDNSIARHGIHGL WL++V I  + ++EGD N I+LTQ R   PFQ IMYDYIRLEGP ++SS  +
Sbjct:   10 IQDRHVMMDNGIVQVTLSNPDGIVTGIRFNGIDNLLEIRNEEINRGYWDLVWSS-ASTGTTGIFDVIKGTSFRVIMESEEQVELSFTRAWDPSQQGKLVPLNIDKRFIMLRGSSGFYSYAIYEHLREWPGFELGETRIAFKLRKDKFHYMAMADNRQRNMPLPDDRLPSRGQALAFPEAVLLVNPVEPEFKGEVDDKYQYSCDNKDNKVHGWICTEPPVGFWQITPSNEFRSGGPFKQNLTSHVGPTTLAMFLSAHYSGEDLVPKFAAGEQWKKVFGPVFMYANSAYEGDDPLSLWEDAKSQMMMEVQCWPYSFPASEDFPKSHQRGSVSGRLLIRDRYI-SDDYIFAQGAYVGLAPQGDVGSWQRECKNYQFWTTADENGYFLIDGIRTGDYNLNATVPGFIGDYQYEVVLTITSGCEVEMGDLVYEPPRNGPTLWEIGIPDRSAAEFYVPDPNPKYINKLFVDHPDRFRQYGLWERYSDLYPEKDLVYTVGVSDYSKDWFFAQVNRKKDDNKYQGTTWQIKFNLDTVDQSGPYKLRLAIASATLSELQVRVNDPKAYPPLFSSGMIGKDNSIARHGIHGLYWLFNVDIPSSRIIEGD-NIIYLTQPRSTSPFQGIMYDYIRLEGPPSLSSKRE 641          

HSP 2 Score: 280.796 bits (717), Expect = 2.293e-72
Identity = 128/183 (69.95%), Postives = 154/183 (84.15%), Query Frame = 1

Query: 1051 DYQFWTRADENGNFTISNVRPGDYNLFAWVPGFVGDYNKFGDIMKITSGSYMELGQCIYKPPRDGPTLWEIGIPDRLAAEFYVPDPNPQHINKLFINHPDRFRQYGLWDRYSELYPDADLVYTIGVSDYRKDWFFAQVPRKREDNTHQGTTWQIKFELSGVIQGTTYTLRVALASATLAELQV 1599
            +YQFWT ADENG F I  +R GDYNL A VPGF+GDY ++  ++ ITSG  +E+G  +Y+PPR+GPTLWEIGIPDR AAEFYVPDPNP++INKLF++HPDRFRQYGLW+RYS+LYP+ DLVYT+GVSDY KDWFFAQV RK++DN +QGTTWQIKF L  V Q   Y LR+A+ASATL+ELQV
Sbjct:  377 NYQFWTTADENGYFLIDGIRTGDYNLNATVPGFIGDY-QYEVVLTITSGCEVEMGDLVYEPPRNGPTLWEIGIPDRSAAEFYVPDPNPKYINKLFVDHPDRFRQYGLWERYSDLYPEKDLVYTVGVSDYSKDWFFAQVNRKKDDNKYQGTTWQIKFNLDTVDQSGPYKLRLAIASATLSELQV 558          

HSP 3 Score: 253.447 bits (646), Expect = 3.921e-64
Identity = 136/244 (55.74%), Postives = 155/244 (63.52%), Query Frame = 1

Query:  496 VVMDNGIVQVTLSKPDGIVTGIKYNGVDNLLEVLNKEPNRGFIML------RGSSGFY------------------------------------------------SYGIY-----EHLRGWPDFDLSETRITFKLRKDKFQYMAVADDRRRIMPFPEDRLPGRCQPLAYQEAVLLVNPKDPRLKGEVDDKYQYSCKNIDNRVHGWISFNPHVGFWQITPSDEFRSGGPLKQNLTSHVGPTTLA 1050
            V+MDNGIVQVTLS PDGIVTGI++NG+DNLLE+ N+E NRG+  L       G++G +                                                S G Y     EHLR WP F+L ETRI FKLRKDKF YMA+AD+R+R MP P+DRLP R Q LA+ EAVLLVNP +P  KGEVDDKYQYSC N DN+VHGWI   P VGFWQITPS+EFRSGGP KQNLTSHVGPTTLA
Sbjct:   15 VMMDNGIVQVTLSNPDGIVTGIRFNGIDNLLEIRNEEINRGYWDLVWSSASTGTTGIFDVIKGTSFRVIMESEEQVELSFTRAWDPSQQGKLVPLNIDKRFIMLRGSSGFYSYAIYEHLREWPGFELGETRIAFKLRKDKFHYMAMADNRQRNMPLPDDRLPSRGQALAFPEAVLLVNPVEPEFKGEVDDKYQYSCDNKDNKVHGWICTEPPVGFWQITPSNEFRSGGPFKQNLTSHVGPTTLA 258          

BLAST of Ca_04975 vs. TrEMBL
Match: B9GN76_POPTR (Predicted protein OS=Populus trichocarpa GN=POPTRDRAFT_551699 PE=4 SV=1)

HSP 1 Score: 1037.71 bits (2682), Expect = 0.000e+0
Identity = 491/627 (78.31%), Postives = 548/627 (87.40%), Query Frame = 1

Query: 1603 MDNGILRVTLSNPEGIVTGIQYNGIDNLLEVLNDESNRGYWDLVWSSPTSTGTTGNFDVIKGSTFKVILEDEDQVELSFTRTWDASMEGKLVPLNIDKRFIMLSGCSGFYTYAIYEHLEDWPAFNLDETRIAFKLRKDKFHYMAMADNRQRNMPLPDDRVPPRGQALAYPEAVLLVNPIEPELKGEVDDKYQYSCDNKDSQVHGWISMDPAVGFWLITPSNEFRSGGPVKQNLTSHVGPTTLAVFLSAHYSGQDLVPKFKAGEAWKKVFGPVFIYLNSPYDGGDPIKLWDDAKLQMLMEVQSWPYSFPESDDFPKWDERGYVSGRLLIKERCIDDDDYVSAKGAYVGLAPPGDVGSWQRECKNYQFWATADYDGYFSISNIHVGDYNVYAWVPGYIGDYKYDVVISITEGCDIDIGDLVYEPLRDGPTLWEIGIPDRSAAEFYVPDPNPKYINKLYVNHPDKFRQYGIWERYAELYPDKDLIYTVGVSDYTKDWFFAQVTRKKDDNTYQGTTWQIKFKLDSVNRSSTYKLRVALASATYSELQVRVNDPKANRPLFSSGLIGKDNSIARHGIHGLNWLYSVGISGNLLVEGDYNTIFLTQARGNGPFQAIMYDYIRLEGPAAISSSN 3483
            MDNGIL+VTLSNP+GIVTGI+Y+GIDNLLEV NDESNRGYWDLVW++    GTTG FDVIKG +FKVI+E E+QVE+SFTRTWD+S EGKLVPLNIDKRF+ML G SGFY+YAIYEHL++WP FNL ETRIAFKLRKDKFHYM  ADNRQR MPLPDDR+PPRGQ LAYPEAV LVNP+EP+ KGEVDDKYQYS +NKD++VHGWI MDPAVGFW ITPS+EFRSGGPVKQNLTSHVGPTTL +FLSAHYSG+DLVPK  AGEAWKKVFGPVF+Y NS  DG DP+ LW+DAKLQML+EVQSWPY FP S+D+ K D+RG VSGRLL+++R +  DDY  A GAYVGLA PGDVGSWQRECK+YQFW  AD  GYFSI+N+  GDYN+YAWVPG IGDY+YDV I+IT GCDI++GDLVYEP RDGPTLWEIGIPDRSA EFYVPDPNPKYINKLYVNHPD+FRQYG+WERYAELYPD DL+YTVGVSDY KDWF+AQVTRKKDDNTYQ  TWQIKF LD V+ +  YKLRVALASAT SELQVR+NDPKA +PLFSSGLIGKDNSIARHGIHGL WLY + + G  LVEGD N +FLTQ R   PFQ IMYDYIRLEGP + SS++
Sbjct:    1 MDNGILQVTLSNPDGIVTGIRYSGIDNLLEVQNDESNRGYWDLVWNT---AGTTGIFDVIKGRSFKVIVETEEQVEVSFTRTWDSSQEGKLVPLNIDKRFVMLRGSSGFYSYAIYEHLKEWPGFNLGETRIAFKLRKDKFHYMIAADNRQRYMPLPDDRLPPRGQPLAYPEAVQLVNPVEPDFKGEVDDKYQYSIENKDNKVHGWICMDPAVGFWQITPSDEFRSGGPVKQNLTSHVGPTTLVMFLSAHYSGEDLVPKIGAGEAWKKVFGPVFMYFNSVMDGDDPLSLWEDAKLQMLIEVQSWPYGFPASEDYQKSDQRGNVSGRLLVRDRFV-SDDYTPANGAYVGLALPGDVGSWQRECKDYQFWNRADEGGYFSINNVRTGDYNLYAWVPGVIGDYRYDVSITITSGCDIEMGDLVYEPPRDGPTLWEIGIPDRSAEEFYVPDPNPKYINKLYVNHPDRFRQYGLWERYAELYPDGDLVYTVGVSDYRKDWFYAQVTRKKDDNTYQRATWQIKFNLDKVDHNGIYKLRVALASATVSELQVRINDPKA-KPLFSSGLIGKDNSIARHGIHGLYWLYGIDVPGARLVEGD-NAVFLTQPRSISPFQGIMYDYIRLEGPPSSSSNS 621          

HSP 2 Score: 287.73 bits (735), Expect = 1.876e-74
Identity = 130/183 (71.04%), Postives = 154/183 (84.15%), Query Frame = 1

Query: 1051 DYQFWTRADENGNFTISNVRPGDYNLFAWVPGFVGDYNKFGDIMKITSGSYMELGQCIYKPPRDGPTLWEIGIPDRLAAEFYVPDPNPQHINKLFINHPDRFRQYGLWDRYSELYPDADLVYTIGVSDYRKDWFFAQVPRKREDNTHQGTTWQIKFELSGVIQGTTYTLRVALASATLAELQV 1599
            DYQFW RADE G F+I+NVR GDYNL+AWVPG +GDY ++   + ITSG  +E+G  +Y+PPRDGPTLWEIGIPDR A EFYVPDPNP++INKL++NHPDRFRQYGLW+RY+ELYPD DLVYT+GVSDYRKDWF+AQV RK++DNT+Q  TWQIKF L  V     Y LRVALASAT++ELQV
Sbjct:  359 DYQFWNRADEGGYFSINNVRTGDYNLYAWVPGVIGDY-RYDVSITITSGCDIEMGDLVYEPPRDGPTLWEIGIPDRSAEEFYVPDPNPKYINKLYVNHPDRFRQYGLWERYAELYPDGDLVYTVGVSDYRKDWFYAQVTRKKDDNTYQRATWQIKFNLDKVDHNGIYKLRVALASATVSELQV 540          

HSP 3 Score: 249.21 bits (635), Expect = 7.394e-63
Identity = 141/284 (49.65%), Postives = 166/284 (58.45%), Query Frame = 1

Query:  502 MDNGIVQVTLSKPDGIVTGIKYNGVDNLLEVLNKEPNRGFIML----RGSSGFY------------------------------------------------SYGIY-----EHLRGWPDFDLSETRITFKLRKDKFQYMAVADDRRRIMPFPEDRLPGRCQPLAYQEAVLLVNPKDPRLKGEVDDKYQYSCKNIDNRVHGWISFNPHVGFWQITPSDEFRSGGPLKQNLTSHVGPTTLADYQFWTRADENGNFTISNVRPGDYNLFAWVPGFVGDYNKFGDIM 1182
            MDNGI+QVTLS PDGIVTGI+Y+G+DNLLEV N E NRG+  L     G++G +                                                S G Y     EHL+ WP F+L ETRI FKLRKDKF YM  AD+R+R MP P+DRLP R QPLAY EAV LVNP +P  KGEVDDKYQYS +N DN+VHGWI  +P VGFWQITPSDEFRSGGP+KQNLTSHVGPTTL     +  A  +G   +  +  G+    AW   F   +  F  +M
Sbjct:    1 MDNGILQVTLSNPDGIVTGIRYSGIDNLLEVQNDESNRGYWDLVWNTAGTTGIFDVIKGRSFKVIVETEEQVEVSFTRTWDSSQEGKLVPLNIDKRFVMLRGSSGFYSYAIYEHLKEWPGFNLGETRIAFKLRKDKFHYMIAADNRQRYMPLPDDRLPPRGQPLAYPEAVQLVNPVEPDFKGEVDDKYQYSIENKDNKVHGWICMDPAVGFWQITPSDEFRSGGPVKQNLTSHVGPTTLV---MFLSAHYSGEDLVPKIGAGE----AWKKVFGPVFMYFNSVM 277          

BLAST of Ca_04975 vs. TrEMBL
Match: F6GZJ7_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VIT_18s0001g07850 PE=4 SV=1)

HSP 1 Score: 1022.31 bits (2642), Expect = 0.000e+0
Identity = 483/637 (75.82%), Postives = 553/637 (86.81%), Query Frame = 1

Query: 1582 LAELQVVMDNGILRVTLSNPEGIVTGIQYNGIDNLLEVLNDESNRGYWDLVWSSPTSTGTTGNFDVIKGSTFKVILEDEDQVELSFTRTWDASMEGKLVPLNIDKRFIMLSGCSGFYTYAIYEHLEDWPAFNLDETRIAFKLRKDKFHYMAMADNRQRNMPLPDDRVPPRGQALAYPEAVLLVNPIEPELKGEVDDKYQYSCDNKDSQVHGWISMDPAVGFWLITPSNEFRSGGPVKQNLTSHVGPTTLAVFLSAHYSGQDLVPKFKAGEAWKKVFGPVFIYLNSPYDGGDPIKLWDDAKLQMLMEVQSWPYSFPESDDFPKWDERGYVSGRLLIKERCIDDDDYVSAKGAYVGLAPPGDVGSWQRECKNYQFWATADYDGYFSISNIHVGDYNVYAWVPGYIGDYKYDVVISIT-EGCDIDIGDLVYEPLRDGPTLWEIGIPDRSAAEFYVPDPNPKYINKLYVNHPDKFRQYGIWERYAELYPDKDLIYTVGVSDYTKDWFFAQVTRKKDDNTYQGTTWQIKFKLDSVNRSSTYKLRVALASATYSELQVRVNDPKANRPLFSSGLIGKDNSIARHGIHGLNWLYSVGISGNLLVEGDYNTIFLTQARGNGPFQAIMYDYIRLEGPAAISSSNKK 3489
            + +  VVMDNGIL+VTLSNP GIVTGI+Y+ IDNLLEVLNDE+NRGYWDLVWS P   G+ G FDVIKG+ F+VI+E+E+QVE+SFTR WD S+EGKLVPLNIDKRFIML G SGFY+YAIYEHL++WPAF L ETRIAFKLRKDKFHYMAMADNRQR MPLPDDR+P RGQ LAYPEAVLLVNP+EPE KGEVDDKYQYSCDNKD++VHGW+ ++P VGFW ITPS+EFR+GGPVKQNLTSHVGPTTLA+F SAHY+G+ LVPKF   E WKKVFGPVFIY+NS +  GDP  LW+DAK QM +EVQSWPYSFP S+DFP  D+RG VSGRLL+++  I  DDY+ A  AYVGLAPPGDVGSWQRECK+YQFW  AD  GYF I++I  GDYN+YAWVPG+IGDYK D VI+IT  GCD+ +GDL+YEP RDGPTLWEIGIPDRSAAEFY PDPNP Y+NKL++ HPD+FRQYG+WERYAELYPD DL+YT+GVSDYTKDWFFAQVTRKKD+NTYQGTTWQIKF LD+V++  TYKLRVA+ASAT SELQVRVNDPKAN P F+SGLIG+DN+IARHGIHGL WLYSV + GNLLVEG+ NTIFLTQ R   PFQ I+YDYIRLEGP + +S++ K
Sbjct:   10 IQDRHVVMDNGILQVTLSNPGGIVTGIRYSAIDNLLEVLNDETNRGYWDLVWSVP---GSAGIFDVIKGTNFRVIVENEEQVEISFTRMWDPSLEGKLVPLNIDKRFIMLRGSSGFYSYAIYEHLKEWPAFTLAETRIAFKLRKDKFHYMAMADNRQRFMPLPDDRLPGRGQPLAYPEAVLLVNPVEPEFKGEVDDKYQYSCDNKDNRVHGWVCLEPPVGFWQITPSDEFRTGGPVKQNLTSHVGPTTLAMFHSAHYAGEPLVPKFGPDEPWKKVFGPVFIYVNSVFGDGDPFWLWEDAKEQMTIEVQSWPYSFPASEDFPSSDQRGNVSGRLLVQDGYI-SDDYILASCAYVGLAPPGDVGSWQRECKDYQFWTQADLGGYFCINDIRPGDYNLYAWVPGFIGDYKLDAVITITPAGCDVFVGDLLYEPPRDGPTLWEIGIPDRSAAEFYSPDPNPTYVNKLFIGHPDRFRQYGLWERYAELYPDGDLVYTIGVSDYTKDWFFAQVTRKKDNNTYQGTTWQIKFTLDTVDQIGTYKLRVAIASATLSELQVRVNDPKANPPHFTSGLIGRDNTIARHGIHGLYWLYSVNVLGNLLVEGE-NTIFLTQPRSTSPFQGILYDYIRLEGPPSPNSNSSK 641          

HSP 2 Score: 283.493 bits (724), Expect = 3.538e-73
Identity = 133/204 (65.20%), Postives = 160/204 (78.43%), Query Frame = 1

Query: 1051 DYQFWTRADENGNFTISNVRPGDYNLFAWVPGFVGDYNKFGDIMKITSGSYMELGQCIYKPPRDGPTLWEIGIPDRLAAEFYVPDPNPQHINKLFINHPDRFRQYGLWDRYSELYPDADLVYTIGVSDYRKDWFFAQVPRKREDNTHQGTTWQIKFELSGVIQGTTYTLRVALASATLAELQVVMDNGILRVTLSNPEGIVTGI 1662
            DYQFWT+AD  G F I+++RPGDYNL+AWVPGF+GDY     I    +G  + +G  +Y+PPRDGPTLWEIGIPDR AAEFY PDPNP ++NKLFI HPDRFRQYGLW+RY+ELYPD DLVYTIGVSDY KDWFFAQV RK+++NT+QGTTWQIKF L  V Q  TY LRVA+ASATL+ELQV +++       +NP    +G+
Sbjct:  375 DYQFWTQADLGGYFCINDIRPGDYNLYAWVPGFIGDYKLDAVITITPAGCDVFVGDLLYEPPRDGPTLWEIGIPDRSAAEFYSPDPNPTYVNKLFIGHPDRFRQYGLWERYAELYPDGDLVYTIGVSDYTKDWFFAQVTRKKDNNTYQGTTWQIKFTLDTVDQIGTYKLRVAIASATLSELQVRVND-----PKANPPHFTSGL 573          

HSP 3 Score: 267.7 bits (683), Expect = 2.009e-68
Identity = 140/242 (57.85%), Postives = 157/242 (64.88%), Query Frame = 1

Query:  496 VVMDNGIVQVTLSKPDGIVTGIKYNGVDNLLEVLNKEPNRGFIML----RGSSGFY-----------------------------------------------------SYGIYEHLRGWPDFDLSETRITFKLRKDKFQYMAVADDRRRIMPFPEDRLPGRCQPLAYQEAVLLVNPKDPRLKGEVDDKYQYSCKNIDNRVHGWISFNPHVGFWQITPSDEFRSGGPLKQNLTSHVGPTTLA 1050
            VVMDNGI+QVTLS P GIVTGI+Y+ +DNLLEVLN E NRG+  L     GS+G +                                                     SY IYEHL+ WP F L+ETRI FKLRKDKF YMA+AD+R+R MP P+DRLPGR QPLAY EAVLLVNP +P  KGEVDDKYQYSC N DNRVHGW+   P VGFWQITPSDEFR+GGP+KQNLTSHVGPTTLA
Sbjct:   15 VVMDNGILQVTLSNPGGIVTGIRYSAIDNLLEVLNDETNRGYWDLVWSVPGSAGIFDVIKGTNFRVIVENEEQVEISFTRMWDPSLEGKLVPLNIDKRFIMLRGSSGFYSYAIYEHLKEWPAFTLAETRIAFKLRKDKFHYMAMADNRQRFMPLPDDRLPGRGQPLAYPEAVLLVNPVEPEFKGEVDDKYQYSCDNKDNRVHGWVCLEPPVGFWQITPSDEFRTGGPVKQNLTSHVGPTTLA 256          

BLAST of Ca_04975 vs. TrEMBL
Match: M0S0W3_MUSAM (Uncharacterized protein OS=Musa acuminata subsp. malaccensis PE=4 SV=1)

HSP 1 Score: 1011.91 bits (2615), Expect = 0.000e+0
Identity = 560/1132 (49.47%), Postives = 706/1132 (62.37%), Query Frame = 1

Query:  481 SPHSLVVMDNGIVQVTLSKPDG-----------------------------------------IVTGIKYNGVD---NLLEVLNK---EPNRG-----------FIMLRGSSGFYSYGIYEHLRGWPDFDLSETRITFKLRKDKFQYMAVADDRRRIMPFPEDRLPGRCQPLAYQEAVLLVNPKDPRLKGEVDDKYQYSCKNIDNRVHGWISFNPHVGFWQITPSDEFRSGGPLKQNLTSHVGPTTLADYQFWTRADENGNFTISNVRPGDYNLFAWVPGFV--------GDYNKFGDIMKITSGSYMELGQCIYKPP--RDGPTLWEIG-IPDRLAAEFYVPDPNPQHINKLFINHPDRFRQYGLWDRYS---ELYPDADLVYTIGVSDYRKDWFFAQVPRKREDNTHQGTTWQIKFELSGVIQGTTYTLRVALASATLAELQVVMDNGILRVTLSNPEGIV---TGIQYNGI-------------------DNLLEVLNDES---------NRGYWDLVWSSPTSTGT-------------------TGNFDVI--------------KGSTFKVILEDEDQVELSFTRTWDASMEGKLVPLNIDKRFIMLSGCSGFYTYAIYEHLEDWPAFNLDETRIAFKLRKDKFHYMAMADNRQRNMPLPDDRVPPRGQALAYPEAVLLVNPIEPELKGEVDDKYQYSCDNKDSQVHGWISMDPAVGFWLITPSNEFRSGGPVKQNLTSHVGPTTLAVFLSAHYSGQDLVPKFKAGEAWKKVFGPVFIYLNSPYDGGDPIKLWDDAKLQMLMEVQSWPYSFPESDDFPKWDERGYVSGRLLIKERCIDDDDYVSAKGAYVGLAPPGDVGSWQRECKNYQFWATADYDGYFSISNIHVGDYNVYAWVPGYIGDYKYDVVISITE-GCDIDIGDLVYEPLRDGPTLWEIGIPDRSAAEFYVPDPNPKYINKLYVNHPDKFRQYGIWERYAELYPDKDLIYTVGVSDYTKDWFFAQVTRKKDDNTYQGTTWQIKFKLDSVNRSSTYKLRVALASATYSELQVRVNDPKANRPLFSSGLIGKDNSIARHGIHGLNWLYSVGISGNLLVEGDYNTIFLTQARGNGPFQAIMYDYIRLEGPA 3465
            SP   VV+DNGIVQ+TLSKP G                                         I+ G ++  V    N +EV  +   +P+ G           F+MLRGSSGFY+Y IYEHL+GWPDF+L ETR+ FKLRKD F YMA+AD+R+RIMP PEDR  GR + L Y EAV L NP +  L+GEVDDKYQYSC N D+ VHGWIS +P VGFW ITPS+EF+SGGP+KQ+LTSH GPT+LA    +  +   G+  +  VR G+Y    + P F+         D     +  K    + +E G   YK P  +D     + G +  RL       + +  + N  F+       + G W R S   + +  ADL      +        A + RK    ++Q TTWQIKF L+ V +  TY LR+ALASA L+E+QV  +N I++       G++     I  +GI                   DN++ +    S         + G   L  S P  + T                    G +D++               G+ F V  +D + VE+SF   WD S  GK VPLNIDKRF+ML G SGFYTYAIYEHL+ WP FNL ETR+ FKLRKDKFH+MA+ADNRQR MP+PDDR   R   L YPEAV L++P    L+GEVDDKYQYS +NKDS VHGWIS DP+VGFW+ITPSNEF+SGGPV+Q+LTSHVGPT L++F+S+HYSG D VPKF+ GE WKKVFGPVFIYLNS  D  DP  LW+DA+ QM +EV+ WPY FP S+DF K ++RG VSGRLL+ ++ I   +Y++   A+VGLA PG+ GSWQRE K YQFW  AD +G F I N+  G YN+YAWVPG+IGDYK  + I++T  G  I++G LVY P RDGPTLWEIGIPDRSAAEF+VPDP+PK+ N+LYV+H D+FRQYG+WERYA+LYPD DLIYT+GVSDY KDWF+AQVTRK    ++Q TTWQI+F+LDSV+ +  YKLRVALA+A  SELQVR N+P+     F++ LIG+DNSIAR GIHGL WL+SV +  + L+EGD N IFLTQ R   PFQ +MYDYIR+E PA
Sbjct:  607 SPFQGVVLDNGIVQLTLSKPGGSITGVKYHGLDNVMEVKNWEDGRGYWDLVWNPSDRDPGIFQIILGTEFKVVQQDANQVEVSFRTQWDPSHGGKLVPLNIDKRFVMLRGSSGFYTYAIYEHLQGWPDFNLGETRVAFKLRKDMFHYMAIADNRQRIMPMPEDREAGRSRRLGYAEAVRLTNPINADLRGEVDDKYQYSCDNKDSMVHGWISSDPSVGFWVITPSNEFKSGGPVKQDLTSHAGPTSLA---IFVSSHYTGDDIVPRVRNGEYWKKVFGPVFIYLNSASTKSDPKLLWEDAK--KQARVEEGSWPYKFPASKDFQKSEQRGSVSGRLLVVDEPANNDSVNGNAAFVGLASP-GEAGSWQRDSKGYQFWERADLTCLTSSA--------ATLSRKHGQASYQATTWQIKFPLNNVHRNGTYKLRLALASAALSEIQVRFNNPIVQQPAHFTTGLIGRDNSIARHGIHGLYWLFSIDVDSSWLLEGDNIIFLTQTRSVSPFQGVLLDNGIVQLTLSKPGGSITGVRYHGLDNVNEVKEKEDGRGYWDLVWNPSDRDSGIFQKILGTEFDVAQQDANHVEVSFRTKWDPSHRGKFVPLNIDKRFVMLRGSSGFYTYAIYEHLQGWPDFNLGETRVVFKLRKDKFHHMAIADNRQRIMPMPDDRRDGRSHKLGYPEAVRLIDPTNAALRGEVDDKYQYSSENKDSLVHGWISSDPSVGFWVITPSNEFKSGGPVRQDLTSHVGPTALSMFVSSHYSGVDTVPKFRNGEYWKKVFGPVFIYLNSAPDKSDPKLLWEDAQKQMQVEVEKWPYEFPASEDFHKSEQRGSVSGRLLVLDKHI-SKEYINGNAAFVGLASPGEAGSWQRESKGYQFWVKADVNGNFFIKNVRTGVYNLYAWVPGFIGDYKSSLNITVTSAGNHINLGSLVYNPPRDGPTLWEIGIPDRSAAEFFVPDPDPKHTNRLYVDHSDRFRQYGLWERYADLYPDGDLIYTIGVSDYRKDWFYAQVTRKDGQGSHQATTWQIRFRLDSVHPNGPYKLRVALAAAHLSELQVRFNNPRVQPAHFTTRLIGRDNSIARQGIHGLYWLFSVDVESSWLLEGD-NIIFLTQTRCQSPFQGVMYDYIRMEAPA 1722          

HSP 2 Score: 809.675 bits (2090), Expect = 0.000e+0
Identity = 387/619 (62.52%), Postives = 475/619 (76.74%), Query Frame = 1

Query: 1588 ELQVVMDNGILRVTLSNPEGIVTGIQYNGIDNLLEVLNDESNRGYWDLVWSSPTSTGTTGNFDVIKGSTFKVILEDEDQVELSFTRTWDASMEGKLVPLNIDKRFIMLSGCSGFYTYAIYEHLEDWPAFNLDETRIAFKLRKDKFHYMAMADNRQRNMPLPDDRVPPRGQALAYPEAVLLVNPIEPELKGEVDDKYQYSCDNKDSQVHGWISMDPAVGFWLITPSNEFRSGGPVKQNLTSHVGPTTLAVFLSAHYSGQDLVPKFKAGEAWKKVFGPVFIYLNSPYDGGDPIKLWDDAKLQMLMEVQSWPYSFPESDDFPKWDERGYVSGRLLIKERCIDDDDYVSAKGAYVGLAPPGDVGSWQRECKNYQFWATADYDGYFSISNIHVGDYNVYAWVPGYIGDYKYDVVISITE-GCDIDIGDLVYEPLRDGPTLWEIGIPDRSAAEFYVPDPNPKYINKLYVNHPDKFRQYGIWERYAELYPDKDLIYTVGVSDYTKDWFFAQVTRKKDDNTYQGTTWQIKFKLDSVNRSSTYKLRVALASATYSELQVRVNDPKANRPLFSSGLIGKDNSIARHGIHGLNWLYSVGISGNLLVEGDYNTIFLTQARGNGPFQAIMYD 3441
            +LQVV+DNGI+R+TLS P G +TG++Y+G+DN++EV  +E   GYWD+VW    S   +G FD+I G+ F V+ +D +QVE+SF   W+ S  GKLVPLN+DKRF+ML G SGFYTYAIYEHL+ WP FNL ETR+AFKLRKD+FHYMA+ADNRQR MP+P+DR P R Q L Y EAV L NPI   L+GEVDDKYQYS +NKDS VHGWIS DP+VGFW+ITPS EF+SGGPVKQ+LTSHVGPT LA F+S+HY+G D++P+ + GE WKKVFGPVFIYLNS     DP  LW+DAK Q  +E  SWPY FP S DF K ++RG VSGRL + +    +  YV+   A+VGLA PG+ GSWQRE K YQFW  AD  G F I N+  G YN+YAWVPG+IGDYK  V I++T  G  I++G+L Y P RDGPTLWEIGIPDRSAAEF+VPDP P YIN+LYVNHPD+FRQYG+WERY +LYPD DL+YTVG+S+Y KDWF+A VTRK    +YQ TTWQIKF+L++V RS TYKLR+ALASA +SE+QV +N    +  L +  LIG+DNSIARHGIHGL WL+S+ +  + L++GD N IFLTQ R   PFQ ++ D
Sbjct:    2 DLQVVLDNGIIRLTLSKPGGSITGVKYHGLDNVMEV-KEEDGGGYWDVVWHP--SDRHSGIFDIILGTEFDVVQQDANQVEVSFRTQWNPSHGGKLVPLNVDKRFVMLRGSSGFYTYAIYEHLQGWPDFNLAETRVAFKLRKDRFHYMAIADNRQRIMPMPEDRQPGRSQKLDYSEAVRLTNPINAALRGEVDDKYQYSIENKDSMVHGWISSDPSVGFWVITPSIEFKSGGPVKQDLTSHVGPTALATFVSSHYAGDDILPRVRNGEYWKKVFGPVFIYLNSASTKSDPKLLWEDAKKQARVEEGSWPYKFPASKDFQKREQRGSVSGRLFVVDEPA-NKHYVNGNAAFVGLASPGEAGSWQRESKGYQFWVRADAKGMFFIKNVRTGVYNLYAWVPGFIGDYKSSVNITVTSAGNHINLGNLEYRPPRDGPTLWEIGIPDRSAAEFFVPDPYPCYINRLYVNHPDRFRQYGLWERYTDLYPDHDLMYTVGISNYKKDWFYAHVTRKHGQASYQATTWQIKFRLNNVRRSGTYKLRLALASAVFSEIQVCLNPRFLHLFLLALELIGRDNSIARHGIHGLYWLFSIDVDSSWLLKGD-NIIFLTQTRSESPFQGVVLD 615          

HSP 3 Score: 692.575 bits (1786), Expect = 0.000e+0
Identity = 338/619 (54.60%), Postives = 424/619 (68.50%), Query Frame = 1

Query: 1054 YQFWTRADENGNFTISNVRPGDYNLFAWVPGFVGDYNKFGDIMKITSGSYMELGQCIYKPPRDGPTLWEIGIPDRLAAEFYVPDPNPQHINKLFINHPDRFRQYGLWDRYSELYPDADLVYTIGVSDYRKDWFFAQVPRK---------------REDNTHQGTTWQIKFELSGV----------------------------------IQGTTYTLRVALASATLAELQ----------------VVMDNGILRVTLSNPEGIVTGIQYNGIDNLLEVLNDESNRGYWDLVWSSPTSTGTTGNFDVIKGSTFKVILEDEDQVELSFTRTWDASMEGKLVPLNIDKRFIMLSGCSGFYTYAIYEHLEDWPAFNLDETRIAFKLRKDKFHYMAMADNRQRNMPLPDDRVPPRGQALAYPEAVLLVNPIEPELKGEVDDKYQYSCDNKDSQVHGWISMDPAVGFWLITPSNEFRSGGPVKQNLTSHVGPTTLAVFLSAHYSGQDLVPKFKAGEAWKKVFGPVFIYLNSPYDGGDPIKLWDDAKLQMLMEVQSWPYSFPESDDFPKWDERGYVSGRLLIKERCIDDDDYVSAKGAYVGLAPPGDVGSWQRECKNYQFWATAD 2715
            YQFW RAD  G F I NVR G YNL+AWVPGF+GDY    +I   ++G+++ LG   Y+PPRDGPTLWEIGIPDR AAEF+VPDP P +IN+L++NHPDRFRQYGLW+RY++LYPD DL+YT+G+S+Y+KDWF+A V RK               R +N  +  T++++  L+                                    I G  +   + + S+ L +                  VV+DNGI+++TLS P G +TG++Y+G+DN++EV N E  RGYWDLVW+   S    G F +I G+ FKV+ +D +QVE+SF   WD S  GKLVPLNIDKRF+ML G SGFYTYAIYEHL+ WP FNL ETR+AFKLRKD FHYMA+ADNRQR MP+P+DR   R + L Y EAV L NPI  +L+GEVDDKYQYSCDNKDS VHGWIS DP+VGFW+ITPSNEF+SGGPVKQ+LTSH GPT+LA+F+S+HY+G D+VP+ + GE WKKVFGPVFIYLNS     DP  LW+DAK Q  +E  SWPY FP S DF K ++RG VSGRLL+ +   ++D  V+   A+VGLA PG+ GSWQR+ K YQFW  AD
Sbjct:  366 YQFWVRADAKGMFFIKNVRTGVYNLYAWVPGFIGDYKSSVNITVTSAGNHINLGNLEYRPPRDGPTLWEIGIPDRSAAEFFVPDPYPCYINRLYVNHPDRFRQYGLWERYTDLYPDHDLMYTVGISNYKKDWFYAHVTRKHGQASYQATTWQIKFRLNNVRRSGTYKLRLALASAVFSEIQVCLNPRFLHLFLLALELIGRDNSIARHGIHGLYWLFSIDVDSSWLLKGDNIIFLTQTRSESPFQGVVLDNGIVQLTLSKPGGSITGVKYHGLDNVMEVKNWEDGRGYWDLVWNP--SDRDPGIFQIILGTEFKVVQQDANQVEVSFRTQWDPSHGGKLVPLNIDKRFVMLRGSSGFYTYAIYEHLQGWPDFNLGETRVAFKLRKDMFHYMAIADNRQRIMPMPEDREAGRSRRLGYAEAVRLTNPINADLRGEVDDKYQYSCDNKDSMVHGWISSDPSVGFWVITPSNEFKSGGPVKQDLTSHAGPTSLAIFVSSHYTGDDIVPRVRNGEYWKKVFGPVFIYLNSASTKSDPKLLWEDAKKQARVEEGSWPYKFPASKDFQKSEQRGSVSGRLLVVDEPANNDS-VNGNAAFVGLASPGEAGSWQRDSKGYQFWERAD 981          

HSP 4 Score: 243.047 bits (619), Expect = 5.299e-61
Identity = 130/276 (47.10%), Postives = 171/276 (61.96%), Query Frame = 1

Query:  496 VVMDNGIVQVTLSKPDGIVTGIKY----------------------------NGVDNLL-----EVLNKEPN------------------------RGFIMLRGSSGFYSYGIYEHLRGWPDFDLSETRITFKLRKDKFQYMAVADDRRRIMPFPEDRLPGRCQPLAYQEAVLLVNPKDPRLKGEVDDKYQYSCKNIDNRVHGWISFNPHVGFWQITPSDEFRSGGPLKQNLTSHVGPTTLADYQFWTRADENGNFTISNVRPGDYNLFAWVPGFV 1152
            VV+DNGI+++TLSKP G +TG+KY                            +G+ +++     +V+ ++ N                        + F+MLRGSSGFY+Y IYEHL+GWPDF+L+ETR+ FKLRKD+F YMA+AD+R+RIMP PEDR PGR Q L Y EAV L NP +  L+GEVDDKYQYS +N D+ VHGWIS +P VGFW ITPS EF+SGGP+KQ+LTSHVGPT LA +     +   G+  +  VR G+Y    + P F+
Sbjct:    5 VVLDNGIIRLTLSKPGGSITGVKYHGLDNVMEVKEEDGGGYWDVVWHPSDRHSGIFDIILGTEFDVVQQDANQVEVSFRTQWNPSHGGKLVPLNVDKRFVMLRGSSGFYTYAIYEHLQGWPDFNLAETRVAFKLRKDRFHYMAIADNRQRIMPMPEDRQPGRSQKLDYSEAVRLTNPINAALRGEVDDKYQYSIENKDSMVHGWISSDPSVGFWVITPSIEFKSGGPVKQDLTSHVGPTALATF---VSSHYAGDDILPRVRNGEYWKKVFGPVFI 277          

HSP 5 Score: 173.326 bits (438), Expect = 5.156e-40
Identity = 104/285 (36.49%), Postives = 155/285 (54.39%), Query Frame = 1

Query:  475 TLSPHSLVVMDNGIVQVTLSKPDGIVTGIKYNGVDNLLEVLNKEPNRGFIMLRGSSGFYSYGIYEHLRGWPDFDLSE-----TRITFKLRKD---KFQYMAVADDRR---------------------------------------------------RIMPFPEDRLPGRCQPLAYQEAVLLVNPKDPRLKGEVDDKYQYSCKNIDNRVHGWISFNPHVGFWQITPSDEFRSGGPLKQNLTSHVGPTTLADYQFWTRADENGNFTISNVRPGDYNLFAWVPGFV 1152
            ++SP   V++DNGIVQ+TLSKP G +TG++Y+G+DN+ EV  KE  RG+  L  +      GI++ + G  +FD+++       ++F+ + D   + +++ +  D+R                                                   RIMP P+DR  GR   L Y EAV L++P +  L+GEVDDKYQYS +N D+ VHGWIS +P VGFW ITPS+EF+SGGP++Q+LTSHVGPT L+    +  +  +G  T+   R G+Y    + P F+
Sbjct: 1096 SVSPFQGVLLDNGIVQLTLSKPGGSITGVRYHGLDNVNEVKEKEDGRGYWDLVWNPSDRDSGIFQKILG-TEFDVAQQDANHVEVSFRTKWDPSHRGKFVPLNIDKRFVMLRGSSGFYTYAIYEHLQGWPDFNLGETRVVFKLRKDKFHHMAIADNRQRIMPMPDDRRDGRSHKLGYPEAVRLIDPTNAALRGEVDDKYQYSSENKDSLVHGWISSDPSVGFWVITPSNEFKSGGPVRQDLTSHVGPTALS---MFVSSHYSGVDTVPKFRNGEYWKKVFGPVFI 1376          

BLAST of Ca_04975 vs. TrEMBL
Match: K7KU46_SOYBN (Uncharacterized protein OS=Glycine max PE=4 SV=1)

HSP 1 Score: 978.008 bits (2527), Expect = 0.000e+0
Identity = 455/510 (89.22%), Postives = 487/510 (95.49%), Query Frame = 1

Query: 1579 TLAELQVVMDNGILRVTLSNPEGIVTGIQYNGIDNLLEVLNDESNRGYWDLVWSSPTSTGTTGNFDVIKGSTFKVILEDEDQVELSFTRTWDASMEGKLVPLNIDKRFIMLSGCSGFYTYAIYEHLEDWPAFNLDETRIAFKLRKDKFHYMAMADNRQRNMPLPDDRVPPRGQALAYPEAVLLVNPIEPELKGEVDDKYQYSCDNKDSQVHGWISMDPAVGFWLITPSNEFRSGGPVKQNLTSHVGPTTLAVFLSAHYSGQDLVPKFKAGEAWKKVFGPVFIYLNSPYDGGDPIKLWDDAKLQMLMEVQSWPYSFPESDDFPKWDERGYVSGRLLIKERCIDDDDYVSAKGAYVGLAPPGDVGSWQRECKNYQFWATADYDGYFSISNIHVGDYNVYAWVPGYIGDYKYDVVISITEGCDIDIGDLVYEPLRDGPTLWEIGIPDRSAAEFYVPDPNPKYINKLYVNHPDKFRQYGIWERYAELYPDKDLIYTVGVSDYTKDWFFAQVTRK 3108
            T+ +  VVM+NGIL+VTLSNP GIVTGIQYN IDNLLEVLNDESNRGYWDLVWSSPTSTGT+G FDVIKG+TFKV++E+EDQVELSFTRTWD S EGKLVPLNIDKRF+ML G SGFY+YAIYEHLE+WPAFNLDETRIAFKLRKDKFHYMAMADNRQRNMPLPDDR+PP+G+ALAYPEAVLLVNPIEPELKGEVDDKYQYSCDNKDSQVHGWI MDPAVGFWLITPSNEFRSGGP+KQNLTSHVGPTTLAVFLSAHYSG+DLVPKF +GEAWKKVFGPVFIYLNSPYDG DP+KLW+DAKLQM +EVQSWPYSFPES+DF KWD+RG VSGRLL++ER I +DDYVSAKGAYVGLAPPGDVGSWQRECKNYQFWA AD DGYFS+SNI  GDYNVYAWVPG+IGDYKYDVVI+ITEGCDID+GDLVYEP RDGPTLWEIGIPDRSAAEFYVPDPNPKYINKLYVNHPDKFRQYG+WERYAELYPDKDLIYT+GVSDYTKDWFFAQVTRK
Sbjct:    9 TIQDNHVVMENGILKVTLSNPGGIVTGIQYNDIDNLLEVLNDESNRGYWDLVWSSPTSTGTSGTFDVIKGTTFKVVVENEDQVELSFTRTWDVSREGKLVPLNIDKRFVMLRGSSGFYSYAIYEHLEEWPAFNLDETRIAFKLRKDKFHYMAMADNRQRNMPLPDDRLPPKGKALAYPEAVLLVNPIEPELKGEVDDKYQYSCDNKDSQVHGWICMDPAVGFWLITPSNEFRSGGPLKQNLTSHVGPTTLAVFLSAHYSGEDLVPKFNSGEAWKKVFGPVFIYLNSPYDGTDPLKLWEDAKLQMSVEVQSWPYSFPESEDFAKWDDRGNVSGRLLVRERYI-NDDYVSAKGAYVGLAPPGDVGSWQRECKNYQFWARADDDGYFSVSNIRAGDYNVYAWVPGFIGDYKYDVVINITEGCDIDLGDLVYEPPRDGPTLWEIGIPDRSAAEFYVPDPNPKYINKLYVNHPDKFRQYGLWERYAELYPDKDLIYTIGVSDYTKDWFFAQVTRK 517          

HSP 2 Score: 38.1206 bits (87), Expect = 0.000e+0
Identity = 17/19 (89.47%), Postives = 18/19 (94.74%), Query Frame = 2

Query: 3107 RKMIIHIREPHGKSSLSLI 3163
            RKMI HI+EPHGKSSLSLI
Sbjct:  516 RKMITHIKEPHGKSSLSLI 534          

HSP 3 Score: 243.047 bits (619), Expect = 5.299e-61
Identity = 134/245 (54.69%), Postives = 154/245 (62.86%), Query Frame = 1

Query:  496 VVMDNGIVQVTLSKPDGIVTGIKYNGVDNLLEVLNKEPNRGFIML-------RGSSGFY------------------------------------------------SYGIY-----EHLRGWPDFDLSETRITFKLRKDKFQYMAVADDRRRIMPFPEDRLPGRCQPLAYQEAVLLVNPKDPRLKGEVDDKYQYSCKNIDNRVHGWISFNPHVGFWQITPSDEFRSGGPLKQNLTSHVGPTTLA 1050
            VVM+NGI++VTLS P GIVTGI+YN +DNLLEVLN E NRG+  L        G+SG +                                                S G Y     EHL  WP F+L ETRI FKLRKDKF YMA+AD+R+R MP P+DRLP + + LAY EAVLLVNP +P LKGEVDDKYQYSC N D++VHGWI  +P VGFW ITPS+EFRSGGPLKQNLTSHVGPTTLA
Sbjct:   15 VVMENGILKVTLSNPGGIVTGIQYNDIDNLLEVLNDESNRGYWDLVWSSPTSTGTSGTFDVIKGTTFKVVVENEDQVELSFTRTWDVSREGKLVPLNIDKRFVMLRGSSGFYSYAIYEHLEEWPAFNLDETRIAFKLRKDKFHYMAMADNRQRNMPLPDDRLPPKGKALAYPEAVLLVNPIEPELKGEVDDKYQYSCDNKDSQVHGWICMDPAVGFWLITPSNEFRSGGPLKQNLTSHVGPTTLA 259          

HSP 4 Score: 239.195 bits (609), Expect = 7.652e-60
Identity = 101/141 (71.63%), Postives = 125/141 (88.65%), Query Frame = 1

Query: 1051 DYQFWTRADENGNFTISNVRPGDYNLFAWVPGFVGDYNKFGDIMKITSGSYMELGQCIYKPPRDGPTLWEIGIPDRLAAEFYVPDPNPQHINKLFINHPDRFRQYGLWDRYSELYPDADLVYTIGVSDYRKDWFFAQVPRK 1473
            +YQFW RAD++G F++SN+R GDYN++AWVPGF+GDY K+  ++ IT G  ++LG  +Y+PPRDGPTLWEIGIPDR AAEFYVPDPNP++INKL++NHPD+FRQYGLW+RY+ELYPD DL+YTIGVSDY KDWFFAQV RK
Sbjct:  378 NYQFWARADDDGYFSVSNIRAGDYNVYAWVPGFIGDY-KYDVVINITEGCDIDLGDLVYEPPRDGPTLWEIGIPDRSAAEFYVPDPNPKYINKLYVNHPDKFRQYGLWERYAELYPDKDLIYTIGVSDYTKDWFFAQVTRK 517          

BLAST of Ca_04975 vs. TrEMBL
Match: K4BUA2_SOLLC (Uncharacterized protein OS=Solanum lycopersicum GN=Solyc04g076640.1 PE=4 SV=1)

HSP 1 Score: 990.719 bits (2560), Expect = 0.000e+0
Identity = 458/623 (73.52%), Postives = 538/623 (86.36%), Query Frame = 1

Query: 1597 VVMDNGILRVTLSNPEGIVTGIQYNGIDNLLEVLNDESNRGYWDLVWSSPTSTGTTGNFDVIKGSTFKVILEDEDQVELSFTRTWDASMEGKLVPLNIDKRFIMLSGCSGFYTYAIYEHLEDWPAFNLDETRIAFKLRKDKFHYMAMADNRQRNMPLPDDRVPPRGQALAYPEAVLLVNPIEPELKGEVDDKYQYSCDNKDSQVHGWISMDPAVGFWLITPSNEFRSGGPVKQNLTSHVGPTTLAVFLSAHYSGQDLVPKFKAGEAWKKVFGPVFIYLNSPYDGGDPIKLWDDAKLQMLMEVQSWPYSFPESDDFPKWDERGYVSGRLLIKERCIDDDDYVSAKGAYVGLAPPGDVGSWQRECKNYQFWATADYDGYFSISNIHVGDYNVYAWVPGYIGDYKYDVVISITEGCDIDIGDLVYEPLRDGPTLWEIGIPDRSAAEFYVPDPNPKYINKLYVNHPDKFRQYGIWERYAELYPDKDLIYTVGVSDYTKDWFFAQVTRKKDDNTYQGTTWQIKFKLDSVNRSSTYKLRVALASATYSELQVRVND-PKANRPLFSSGLIGKDNSIARHGIHGLNWLYSVGISGNLLVEGDYNTIFLTQARGNGPFQAIMYDYIRLEGP 3462
            VVMDNGIL+VTLS P+GIVTGI YNG+DNLLEVLNDE+NRGYWD+VWSS  +TGTTG F+ +  +T+KVILE +DQ+ELSFTRTWD S++ KL+PLNIDKRFIML G SGFY+YAIYEHLE WP FNLDETRIAFKLRKDKFHYMAMADNRQR MPLPDDR+P RGQ LAYPEAVLLVNPIEPE KGEVDDKYQYSC++KD +VHGWI MDP++GFW+I PS+EFRSGGP+KQNLTSHVGPT L+VFLSAHY+G+DLVPKF  GE WKKVFGPVFIYLNS   G DP+ LW+DAK QMLMEVQSWPYSFP S+DFP   +RG +SGRLL+ +R   DD  +SA GA++GLAPPG+ GSWQRECK+YQFW  AD +G + ISNI  GDYN+YA+VPG+IGDYKYD+ I+IT GC I++ DLV+EP R+GPTLWEIGIPDRSA EFY+PDP+ KY+NKL++NHPD+FRQYG+WERYA+LYP+ D+++T+G SDY KDWFF QVTRK D+ TYQGTTWQIKFKLD+V+++  Y LR+ALASA  +ELQVR+ND    N PLFSSG+IGKDN+IARHGIHGL WL++V + G +LVEG+ NTI+LTQA    PFQ IMYDYIRLEGP
Sbjct:   16 VVMDNGILQVTLSVPDGIVTGITYNGVDNLLEVLNDETNRGYWDVVWSSTGTTGTTGTFERLICTTYKVILETDDQIELSFTRTWDVSLQDKLIPLNIDKRFIMLKGSSGFYSYAIYEHLEGWPGFNLDETRIAFKLRKDKFHYMAMADNRQRYMPLPDDRLPDRGQPLAYPEAVLLVNPIEPEFKGEVDDKYQYSCEDKDLKVHGWICMDPSLGFWIIIPSDEFRSGGPLKQNLTSHVGPTALSVFLSAHYAGEDLVPKFGQGEPWKKVFGPVFIYLNSVTSGEDPLTLWEDAKKQMLMEVQSWPYSFPASEDFPSSSQRGNISGRLLVLDRYFKDDK-ISASGAFIGLAPPGEAGSWQRECKDYQFWTRADEEGNYVISNIRAGDYNLYAFVPGFIGDYKYDINITITSGCSIEMDDLVFEPPRNGPTLWEIGIPDRSAREFYIPDPDQKYVNKLFINHPDRFRQYGLWERYADLYPNGDIVFTIGESDYKKDWFFVQVTRKTDEKTYQGTTWQIKFKLDNVDQNEIYTLRLALASAAQAELQVRINDNSSTNTPLFSSGVIGKDNAIARHGIHGLYWLFNVNLQGTILVEGE-NTIYLTQANATSPFQGIMYDYIRLEGP 636          

HSP 2 Score: 277.33 bits (708), Expect = 2.535e-71
Identity = 133/220 (60.45%), Postives = 166/220 (75.45%), Query Frame = 1

Query: 1051 DYQFWTRADENGNFTISNVRPGDYNLFAWVPGFVGDYNKFGDIMKITSGSYMELGQCIYKPPRDGPTLWEIGIPDRLAAEFYVPDPNPQHINKLFINHPDRFRQYGLWDRYSELYPDADLVYTIGVSDYRKDWFFAQVPRKREDNTHQGTTWQIKFELSGVIQGTTYTLRVALASATLAELQV-VMDNGILRVTL--SNPEGIVTGIQYNGIDNLLEVLN 1701
            DYQFWTRADE GN+ ISN+R GDYNL+A+VPGF+GDY K+   + ITSG  +E+   +++PPR+GPTLWEIGIPDR A EFY+PDP+ +++NKLFINHPDRFRQYGLW+RY++LYP+ D+V+TIG SDY+KDWFF QV RK ++ T+QGTTWQIKF+L  V Q   YTLR+ALASA  AELQV + DN      L  S   G    I  +GI  L  + N
Sbjct:  379 DYQFWTRADEEGNYVISNIRAGDYNLYAFVPGFIGDY-KYDINITITSGCSIEMDDLVFEPPRNGPTLWEIGIPDRSAREFYIPDPDQKYVNKLFINHPDRFRQYGLWERYADLYPNGDIVFTIGESDYKKDWFFVQVTRKTDEKTYQGTTWQIKFKLDNVDQNEIYTLRLALASAAQAELQVRINDNSSTNTPLFSSGVIGKDNAIARHGIHGLYWLFN 597          

HSP 3 Score: 236.113 bits (601), Expect = 6.478e-59
Identity = 109/147 (74.15%), Postives = 124/147 (84.35%), Query Frame = 1

Query:  610 NRGFIMLRGSSGFYSYGIYEHLRGWPDFDLSETRITFKLRKDKFQYMAVADDRRRIMPFPEDRLPGRCQPLAYQEAVLLVNPKDPRLKGEVDDKYQYSCKNIDNRVHGWISFNPHVGFWQITPSDEFRSGGPLKQNLTSHVGPTTLA 1050
            ++ FIML+GSSGFYSY IYEHL GWP F+L ETRI FKLRKDKF YMA+AD+R+R MP P+DRLP R QPLAY EAVLLVNP +P  KGEVDDKYQYSC++ D +VHGWI  +P +GFW I PSDEFRSGGPLKQNLTSHVGPT L+
Sbjct:  114 DKRFIMLKGSSGFYSYAIYEHLEGWPGFNLDETRIAFKLRKDKFHYMAMADNRQRYMPLPDDRLPDRGQPLAYPEAVLLVNPIEPEFKGEVDDKYQYSCEDKDLKVHGWICMDPSLGFWIIIPSDEFRSGGPLKQNLTSHVGPTALS 260          

HSP 4 Score: 74.7146 bits (182), Expect = 2.496e-10
Identity = 36/42 (85.71%), Postives = 38/42 (90.48%), Query Frame = 1

Query:  496 VVMDNGIVQVTLSKPDGIVTGIKYNGVDNLLEVLNKEPNRGF 621
            VVMDNGI+QVTLS PDGIVTGI YNGVDNLLEVLN E NRG+
Sbjct:   16 VVMDNGILQVTLSVPDGIVTGITYNGVDNLLEVLNDETNRGY 57          

BLAST of Ca_04975 vs. DB:Swiss
Match: RHIE_DICD3 (Rhamnogalacturonate lyase OS=Dickeya dadantii (strain 3937) OX=198628 GN=rhiE PE=1 SV=1)

HSP 1 Score: 115.161 bits (287), Expect = 5.654e-25
Identity = 149/593 (25.13%), Postives = 240/593 (40.47%), Query Frame = 1

Query: 1579 TLAELQVVMDNGILRVTLSNPEGIVTGIQYNGIDNLLEVLND-----ESNRGYWDLVWSSPTSTGTTGNFDVIKGSTFKVILEDEDQVELSFTRTWDASMEGKLVPLNIDKRFIMLSGCSGFYTYAIYEHLEDWPAFNLDETRIAFKLRKDKFHYMAMADNRQRNMPLPDDRVPPRGQALAYPEAVLLVNPIEPEL-KGEVDDKYQYSCDNKDSQVHGWISMDPAVGFWLITPSNEFRSGGPVKQNLTSHVGPTTLAVFLSAHYSGQDLVPKFKAGEAWKKVFGPVFIYLNSPYDGGDPIKLWDDAKLQMLMEVQSWPYSFPESDDFPKWDERGYVSGRLLIKERCIDDDDYVSAKGAYVGLAPPGDVGSWQRECKNYQFWATADYDGYFSISNIHVGDYNVYAWVPG--YIGDYKYDVVISITEGCDIDIGDLVYEPLRDGPTLWEIGIPDRSAAEFYVPDPNPKYINKLYVNHPDKFRQYGIWERYAELYPDKDLIYTVGVSDYTKDWFFAQVTRKKDDNTYQGTTWQIKFKLDSVNRSSTYKLRVALASATYSELQVRVNDP----KANRPLFSSGLIGKDNSIARHGIH 3321
            TL  +   +DNG+L+V     +G    + + G  NL+  L+      + NR ++   +S        G  +       +VI +  DQV L++         GKL    ++   IM    SG Y+Y +  +    P   + E R  ++   D      + ++ +R  PL  D +          E +  V      L  G V  KY ++   ++S+   W  M    G W++  S E+ SG  +KQ L  H     L     +H+   D+V    A   ++K++GP  +Y+N     G+  +L  D   +   E  SWPY + +   +P+  +R  VSGRL  +           A  A V L    +  ++  +   Y F A  + DG FS+SN+  G+Y + A+  G   IG      V    EG    +G +  +  +  P  W IG  DR A EF                  DK RQY    R+    P  DL + +G S   KDW++AQ          Q  +W I F  ++      Y L +A+A+A+ + +    + P    K N  L ++     D SI R  + 
Sbjct:   32 TLDGMNSTLDNGLLKVRF-GADGSAKEV-WKGGTNLISRLSGAARDPDKNRSFYLDYYS--------GGVNEFVPERLEVIKQTPDQVHLAYI----DDQNGKL---RLEYHLIMTRDVSGLYSYVVAANTGSAP-VTVSELRNVYRF--DATRLDTLFNSIRRGTPLLYDEL----------EQLPKVQDETWRLPDGSVYSKYDFAGYQRESRY--WGVMGNGYGAWMVPASGEYYSGDALKQELLVHQDAIILNYLTGSHFGTPDMV----AQPGFEKLYGPWLLYINQ----GNDRELVADVSRRAEHERASWPYRWLDDARYPR--QRATVSGRLRTE-----------APHATVVLNSSAE--NFDIQTTGYLFSARTNRDGRFSLSNVPPGEYRLSAYADGGTQIGLLAQQTV--RVEGKKTRLGQI--DARQPAPLAWAIGQADRRADEF---------------RFGDKPRQY----RWQTEVP-ADLTFEIGKSRERKDWYYAQT---------QPGSWHILF--NTRTPEQPYTLNIAIAAASNNGMTTPASSPQLAVKLNGQLLTTLKYDNDKSIYRGAMQ 534          

HSP 2 Score: 67.3958 bits (163), Expect = 5.868e-10
Identity = 64/209 (30.62%), Postives = 92/209 (44.02%), Query Frame = 1

Query: 1054 YQFWTRADENGNFTISNVRPGDYNLFAWVPGFVGDYNKFGDIMKIT---SGSYMELGQCIYKPPRDGPTLWEIGIPDRLAAEFYVPDPNPQHINKLFINHPDRFRQYGLWDRYSELYPDADLVYTIGVSDYRKDWFFAQVPRKREDNTHQGTTWQIKFELSGVIQGTTYTLRVALASATLAELQVVMDNGILRVTLSNPEGIVTGIQYN 1671
            Y F  R + +G F++SNV PG+Y L A+  G      + G + + T    G    LGQ   + P   P  W IG  DR A EF                  D+ RQY    R+    P ADL + IG S  RKDW++AQ          Q  +W I F      Q   YTL +A+A+A+   +     +  L V L+    ++T ++Y+
Sbjct:  354 YLFSARTNRDGRFSLSNVPPGEYRLSAYADGGT----QIGLLAQQTVRVEGKKTRLGQIDARQP--APLAWAIGQADRRADEF---------------RFGDKPRQY----RWQTEVP-ADLTFEIGKSRERKDWYYAQT---------QPGSWHILFNTRTPEQ--PYTLNIAIAAASNNGMTTPASSPQLAVKLNGQ--LLTTLKYD 523          

BLAST of Ca_04975 vs. DB:Swiss
Match: RGLB_EMENI (Rhamnogalacturonate lyase B OS=Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) OX=227321 GN=rglB PE=2 SV=2)

HSP 1 Score: 80.4925 bits (197), Expect = 5.048e-14
Identity = 131/602 (21.76%), Postives = 234/602 (38.87%), Query Frame = 1

Query: 1573 SATLAELQVVMDNGILRVTLSNPEGIVTGIQYNGIDNLLEVLNDESNRGYWDLVWSSPTSTGTTGNFDVIKGSTFKVILEDEDQVELSFTRTWDASMEGKLVPL--NIDKRFIMLSGCSGFYTYAIYEHLEDWPAF--NLDETRIAFKLRKDKFHYMAMADNRQRNMPLPDDRVPPRGQALAYPEAVLLVNPIEPELK----GEVDDKYQYSCDNKDSQVHGW-----ISMDPAVGFWLITPSNEFRSGGPVKQNLTSHVGPTTLAVFLSAHYSGQDLVPKFKAGEAWKKVFGPVFIYLNSPYDGGDPIKLWDDAKLQMLMEVQSWPYSFPESD-----DFPKWDERGYVSGRLLIKERCIDDDDYVSAKGAYV--GLAPPGDVGSWQRECKNYQFWATADYDGYFSISNIHVGDYNVYAWVPGYIGDYKYD-VVISITEGCDIDIGDLVYEPLRDGPTLWEIGIPDRSAAEF---YVPDPNPKYINKLYVNHPDKFRQYGIWERYAELYPDKDLIYTVGVSDYTKDW---FFAQVTRKKDDNTYQGTT---WQIKFKLDS----VNRSSTYKLRVALASATYSELQV-RVNDPKANRPLFS 3273
            +AT  +  +V++N  LR T     G +  +  +G D  L   +  +  G +   + +P+   T G+ + +         E     + + T+     +     P      + + +  G +GF+ ++   +  +   F  NL E R  F+   D + ++  +D   +  PLP D      + +   +A   +N    +       E   KY +S   +D+ VHG       S     G WL+  + +   GGP+  +LT  V        +S H+   +  P    G  + + FGP F   N    GG    L +       +   SW   F +S       +    +RG V G + + +        ++  G Y     A P           ++Q+WA  D +G FSI  +  G Y +  +  G  GD+  D +V+   +   I      ++P   G  +W +G PD+S+ EF      DP        +  HP ++  Y     +   +P   + Y +G SD   D+    +A      D+   +  T   W+I+F L +      +++T  +++A A A      V   ++P AN PL S
Sbjct:   21 TATENDTSIVLENDRLRATFDKGRGSIIDLYLDGQD-FLGPQSGSTGIGPYLDCYCTPSGFYTAGSTNPVT--------ELVQGTDSTGTKYAGIILNDTYTPTGQEFQQYWFLRDGETGFHMFSRLAYYNETTPFLRNLQELRTLFRPNTDLWTHLTSSD--LQTAPLPSDEAI--AEQIVVQDATWRLNNTPDDAYYQQFSEYFTKYTFSNHWRDNDVHGLYADGSTSDGTTYGAWLVMNTKDTYYGGPLHSDLT--VDGIIYNYIVSNHHG--EGTPNITNG--FDRTFGPQFYLFN----GGGSSSLEELRDEARSLASPSWNADFYDSIAKHVIGYVPSSQRGSVKGTIKLPKNAKSPIAVLTVDGHYFQDNSAVP----------SSHQYWADIDKNGRFSIDRVVAGKYRLTVYADGIFGDFTRDGIVVKARKSTSI---KETWKPESAGTEIWRLGTPDKSSGEFRHGAARDPT-------HPRHPPEYLIYWGAYDWQSDFP-GGIDYMIGESDPATDFNTVHWAVFGPTPDNPVAESNTTHDWRIRFDLSAKQLHARKTATLTIQLAGAKAASGNTDVYNASEPYANLPLRS 578          

HSP 2 Score: 65.4698 bits (158), Expect = 2.074e-9
Identity = 54/191 (28.27%), Postives = 85/191 (44.50%), Query Frame = 1

Query: 1048 ADYQFWTRADENGNFTISNVRPGDYNLFAWVPGFVGDYNKFGDIMKITSGSYMELGQCIYKPPRDGPTLWEIGIPDRLAAEF-YVPDPNPQHINKLFINHPDRFRQY-GLWDRYSELYPDADLVYTIGVSDYRKDW------FFAQVPRKREDNTHQGTTWQIKFELSG--VIQGTTYTLRVALASATLAE 1590
            + +Q+W   D+NG F+I  V  G Y L  +  G  GD+ + G ++K    + +   +  +KP   G  +W +G PD+ + EF +    +P H       HP  +  Y G +D  S+     D  Y IG SD   D+       F   P      ++    W+I+F+LS   +    T TL + LA A  A 
Sbjct:  379 SSHQYWADIDKNGRFSIDRVVAGKYRLTVYADGIFGDFTRDGIVVKARKSTSI---KETWKPESAGTEIWRLGTPDKSSGEFRHGAARDPTH-----PRHPPEYLIYWGAYDWQSDFPGGID--YMIGESDPATDFNTVHWAVFGPTPDNPVAESNTTHDWRIRFDLSAKQLHARKTATLTIQLAGAKAAS 559          

BLAST of Ca_04975 vs. DB:Swiss
Match: RGLB_ASPFN (Probable rhamnogalacturonate lyase B OS=Aspergillus flavus (strain ATCC 200026 / FGSC A1120 / NRRL 3357 / JCM 12722 / SRRC 167) OX=332952 GN=rglB PE=3 SV=1)

HSP 1 Score: 70.8626 bits (172), Expect = 5.758e-11
Identity = 110/491 (22.40%), Postives = 189/491 (38.49%), Query Frame = 1

Query: 1900 FIMLSGCSGFYTYAIYEHLEDWPAF--NLDETRIAFKLRKDKFHYMAMADNRQRNMPLPDDRVPPRGQALAYPEAVLLVNPIEPELK----GEVDDKYQYSCDNKDSQVHGWI-----SMDPAVGFWLITPSNEFRSGGPVKQNLTSHVGPTTLAVFLSAHYSGQDLVPKFKAGEAWKKVFGPVFIYLNSPYDGGDPIKLWDDAKLQMLMEVQSWPYSFPESD-----DFPKWDERGYVSGRLLIKERCIDDDDYVSAKGAYVGLAPPGDVGSWQRECKNYQFWATADYDGYFSISNIHVGDYNVYAWVPGYIGDYKYDVVISITEGCDIDIGDL-----VYEPLRDGPTLWEIGIPDRSAAEFYVPD-PNPKYINKLYVNHPDKFRQYGIWERYAELYPDKDLIYTVGVSDYTKD-----WFFAQVTRKKDDNTYQGTT-WQIKFKLD--SVNRSSTYKLRVALASATYSELQVRV---NDPKANRPLFS 3273
            + +  G +G +T++   +  +   F  NL E R  F+   + + ++  ++   +  PLP          +   +A    N    +       E   KY +S   +D+ VHG       S     G WL+  + +   GGP+  +LT  V        +S H+   +  P    G  + + FGP +   N     G    L D       +    W   F +S       +    +RG V G++ + +        ++  G Y              E  ++Q+WA    DG F + ++  G Y +  +  G  GD+ +D       G ++  G +      +E    G  +W +G PD+S+ EF   D P+P +     ++ P  F  +G ++ + + +P+  + YT+G SD   D     W     T    D  Y  T  W I F LD   + +  T  L + LA A  +     V    +P AN  L S
Sbjct:  124 WFLRDGETGLHTFSRLAYYNETTPFLRNLQEFRTLFRPNTELWTHLTSSE--AQTAPLPSKEA--IANEVVVQDATWRFNNTPNDAYYTQFSEYFTKYTFSNLWRDNSVHGLYADGTNSNGTTYGAWLVMNTKDTYYGGPLHSDLT--VDGIVYNYLVSNHHG--EGTPNITNG--FDRTFGPQYYLFNG--GKGSKSSLEDLRSEAETLADPGWNADFYDSIAKHVIGYAPSSKRGSVQGQVKLPKGSTRPIAILTVDGQYFQ--------DNSVEASSHQYWAEMGQDGTFQLDHVKEGKYRLTVFADGIFGDFVHD-------GVEVQAGKVTKVQETWEQESAGVEVWRLGTPDKSSGEFLHGDAPDPTHP----LHPPQHFIYWGAYD-WQQDFPNG-VNYTIGSSDPAVDFNTVHWSVYGPTPANPDVEYDTTHDWTINFSLDKKQLQQRKTATLTIQLAGAKTAAGNTDVYNATEPYANLALES 581          

HSP 2 Score: 61.2326 bits (147), Expect = 5.408e-8
Identity = 53/192 (27.60%), Postives = 90/192 (46.88%), Query Frame = 1

Query: 1048 ADYQFWTRADENGNFTISNVRPGDYNLFAWVPGFVGDYNKFGDIMKITSGSYMELGQCIYKPPRDGPTLWEIGIPDRLAAEFYVPD-PNPQHINKLFINHPDRFRQYGLWDRYSELYPDADLVYTIGVSDYRKDW------FFAQVPRKRE---DNTHQGTTWQIKFEL--SGVIQGTTYTLRVALASATLA 1587
            + +Q+W    ++G F + +V+ G Y L  +  G  GD+    D +++ +G   ++ Q  ++    G  +W +G PD+ + EF   D P+P H     ++ P  F  +G +D + + +P+  + YTIG SD   D+       +   P   +   D TH    W I F L    + Q  T TL + LA A  A
Sbjct:  382 SSHQYWAEMGQDGTFQLDHVKEGKYRLTVFADGIFGDF--VHDGVEVQAGKVTKV-QETWEQESAGVEVWRLGTPDKSSGEFLHGDAPDPTHP----LHPPQHFIYWGAYD-WQQDFPNG-VNYTIGSSDPAVDFNTVHWSVYGPTPANPDVEYDTTHD---WTINFSLDKKQLQQRKTATLTIQLAGAKTA 561          

BLAST of Ca_04975 vs. DB:Swiss
Match: RGLB_ASPTN (Probable rhamnogalacturonate lyase B OS=Aspergillus terreus (strain NIH 2624 / FGSC A1156) OX=341663 GN=rglB PE=3 SV=1)

HSP 1 Score: 70.4774 bits (171), Expect = 7.108e-11
Identity = 109/477 (22.85%), Postives = 180/477 (37.74%), Query Frame = 1

Query: 1900 FIMLSGCSGFYTYAIYEHLEDWPAF--NLDETRIAFKLRKDKFHYMAMADNRQRNMPLPDDRVPPRGQALAYPEAVLLVNPIEPELK----GEVDDKYQYSCDNKDSQVHGW-----ISMDPAVGFWLITPSNEFRSGGPVKQNLTSHVGPTTLAVFLSAHYSGQDLVPKFKAGEAWKKVFGPVFIYLNSPYDGGDPIKLWDDAKLQMLMEVQSWPYSFPESD-----DFPKWDERGYVSGRLLIKERCIDDDDYVSAKGAYVGLAPPGDVGSWQRECKNYQFWATADYDGYFSISNIHVGDYNVYAWVPGYIGDYKYDVVISITEGCDIDIGDLVYEPLRDGPTLWEIGIPDRSAAEFY---VPDPNPKYINKLYVNHPDKFRQYGIWERYAELYPDKDLIYTVGVSDYTKD-----WFFAQVTRKKDDNTYQGTT-WQIKFKL--DSVNRSSTYKLRVALASATYSELQVRVNDP 3249
            + +  G +G + ++   +  +   F  NL E R  F+   D + ++  ++   +  PLP D     G+ +   +A    N    +       E   KY +S   +D+ VHG       S     G WL+  + +   GGP+  +LT  V        +S H+   +  P    G  + + FGP F   N    GG    L +       +   SW   F +S       +    +RG V G++ +          ++  G Y              + ++YQ+W   D +G F + ++  G Y +  +  G  GDY  D V  +       I D  ++P   G  +W +G PD+S+ EF      DP           HP ++  Y     + + +P+  + YT+G SD   D     W     T    D  Y  T  W I F L    + +  T  L + LA A  +     V  P
Sbjct:  123 WFLRDGETGLHMFSRLAYYNETTPFLRNLQEFRTLFRPNTDLWTHLTSSE--LQTAPLPSDEA--VGKQVVVQDATWRFNNTPNDAYYTQFSEYFTKYTFSNAWRDNNVHGLYADGSTSNGTTFGAWLVMNTKDTYYGGPLHSDLT--VDGIVYNYIVSNHHG--EGTPNITNG--FDRTFGPQFYLFN----GGGSSSLNELRSEAESLADPSWNVEFYDSIAKHVVGYVPSSKRGSVQGQIKLPRGATRPIAILTVDGQYFQ--------DNSVDPRSYQYWVEMDANGKFQLDHVVEGKYRLTVYADGIFGDYVRDGV-QVRGRKTTRIND-SWQPESAGVEVWRLGTPDKSSGEFLHGVARDPTHPL-------HPPEYLIYWGAYDWQQDFPNG-VNYTIGSSDPATDFNTVHWSVFGPTPDNPDVEYDTTHDWAINFSLTKKQLQKRKTATLTIQLAGAKTASGNTDVYKP 567          

HSP 2 Score: 57.3806 bits (137), Expect = 7.689e-7
Identity = 54/191 (28.27%), Postives = 85/191 (44.50%), Query Frame = 1

Query: 1054 YQFWTRADENGNFTISNVRPGDYNLFAWVPGFVGDYNKFGDIMKITSGSYMELGQCIYKPPRDGPTLWEIGIPDRLAAEF-YVPDPNPQHINKLFINHPDRFRQYGLWDRYSELYPDADLVYTIGVSDYRKDW------FFAQVPRKRE---DNTHQGTTWQIKFELSG--VIQGTTYTLRVALASATLAE 1590
            YQ+W   D NG F + +V  G Y L  +  G  GDY + G  ++   G         ++P   G  +W +G PD+ + EF +    +P H     ++ P+    +G +D + + +P+  + YTIG SD   D+       F   P   +   D TH    W I F L+   + +  T TL + LA A  A 
Sbjct:  381 YQYWVEMDANGKFQLDHVVEGKYRLTVYADGIFGDYVRDGVQVR---GRKTTRINDSWQPESAGVEVWRLGTPDKSSGEFLHGVARDPTHP----LHPPEYLIYWGAYD-WQQDFPNG-VNYTIGSSDPATDFNTVHWSVFGPTPDNPDVEYDTTHD---WAINFSLTKKQLQKRKTATLTIQLAGAKTAS 559          

BLAST of Ca_04975 vs. DB:Swiss
Match: RGLB_ASPNC (Probable rhamnogalacturonate lyase B OS=Aspergillus niger (strain CBS 513.88 / FGSC A1513) OX=425011 GN=rglB PE=3 SV=1)

HSP 1 Score: 69.3218 bits (168), Expect = 1.383e-10
Identity = 106/471 (22.51%), Postives = 182/471 (38.64%), Query Frame = 1

Query: 1885 NIDKRFIMLSGCSGFYTYAIYEHLEDWPAF--NLDETRIAFKLRKDKFHYMAMADNRQRNMPLPDDRVPPRGQALAYPEAVLLVNPIEPELKGEVD--DKYQYSCDNKDSQVHGW-----ISMDPAVGFWLITPSNEFRSGGPVKQNLTSHVGPTTLAVFLSAHYSGQDLVPKFKAGEAWKKVFGPVFIYLNSPYDGGDPIKLWDDAKLQMLMEVQSWPYSFPESD-----DFPKWDERGYVSGRLLIKERCIDDDDYVSAKGAYVGLAPPGDVGSWQRECKNYQFWATADYDGYFSISNIHVGDYNVYAWVPGYIGDYKYDVVISITEGCDIDIGDLVYEPLRDGPTLWEIGIPDRSAAEF---YVPDPNPKYINKLYVNHPDKFRQYGIWERYAELYPDKDLIYTVGVSDYTKD-----WFFAQVT----RKKDDNTYQGTTWQIKFKL--DSVNRSSTYKLRVALASA 3213
            +  + + +  G +G +T++   +  +   +  NL E R  F+   + + +++ ++   +  PLP  +       +          P +       D   KY +S   +D+ VHG       S     G WL+  + +   GGP+  +LT  V        +S H+   +  P    G  + + FGP + + N     G    L +       +   SW   F +S       +    +RG V G++ + +        ++  G Y        V S      +YQ+WA  D  G+FS+ ++  G Y +  +  G  GD+  D V  +  G    I +  +E    G  +W +G PD+S+ EF      DP        +  HP ++  Y     +   +PD  + YT+G SD   D     W     T    R + D T+    W I F L  D +   S   L + LA A
Sbjct:  119 HFQQYWFLRDGETGLHTFSRLAYYNETTPYLRNLQEFRTLFRPNTELWTHLSSSE--VQTAPLPSKKAVEEEVVVQDATWTFNNTPTDEYYVQFADYFTKYTFSNAWRDNSVHGMYADGSTSNGSTFGAWLVMNTKDTYYGGPLHSDLT--VDGIVYNYLVSNHHG--EGTPNITYG--FDRTFGPQYYHFNG--GKGSTASLQELKSDAETLADPSWNVDFYDSIAKHVVGYTPSSQRGSVQGKIKLPKGATRPIAVLTVDGQYFQ---DNSVNS-----SSYQYWAEIDDSGHFSVDHVKEGPYRLTVYADGIFGDFVRDGV-QVKAGKKTTIQE-TWEAESAGTEIWRLGTPDKSSGEFRHGVARDPT-------HPLHPPEYLIYWGAYDWQSDFPDG-INYTIGTSDPATDLNTVHWSVFGPTPNDPRVEYDTTHD---WTINFPLSEDDLAERSKATLTIQLAGA 558          

HSP 2 Score: 64.3142 bits (155), Expect = 5.448e-9
Identity = 56/192 (29.17%), Postives = 93/192 (48.44%), Query Frame = 1

Query: 1048 ADYQFWTRADENGNFTISNVRPGDYNLFAWVPGFVGDYNKFGDIMKITSGSYMELGQCIYKPPRDGPTLWEIGIPDRLAAEF-YVPDPNPQHINKLFINHPDRFRQYGLWDRYSELYPDADLVYTIGVSDYRKD-----W-FFAQV---PRKREDNTHQGTTWQIKFELS--GVIQGTTYTLRVALASATLA 1587
            + YQ+W   D++G+F++ +V+ G Y L  +  G  GD+ +  D +++ +G    + Q  ++    G  +W +G PD+ + EF +    +P H     ++ P+    +G +D  S+ +PD  + YTIG SD   D     W  F      PR   D TH    W I F LS   + + +  TL + LA A  A
Sbjct:  382 SSYQYWAEIDDSGHFSVDHVKEGPYRLTVYADGIFGDFVR--DGVQVKAGKKTTI-QETWEAESAGTEIWRLGTPDKSSGEFRHGVARDPTHP----LHPPEYLIYWGAYDWQSD-FPDG-INYTIGTSDPATDLNTVHWSVFGPTPNDPRVEYDTTHD---WTINFPLSEDDLAERSKATLTIQLAGAKAA 561