Development and Characterization of Microsatellite Markers from Chromosome 1-Specific DNA Libraries of Vicia faba

Publication Overview
TitleDevelopment and Characterization of Microsatellite Markers from Chromosome 1-Specific DNA Libraries of Vicia faba
AuthorsPozarkova D, Koblizkova A, Roman B, Torres AM, Lucretti S, Lysak M, Dolezel J, Macas J
TypeJournal Article
Journal NameBiologia Plantarum
Volume45
Issue3
Year2002
Page(s)337-345
CitationPozarkova D, Koblizkova A, Roman B, Torres AM, Lucretti S, Lysak M, Dolezel J, Macas J. Development and Characterization of Microsatellite Markers from Chromosome 1-Specific DNA Libraries of Vicia faba. 2002; 45(3):337-345.

Abstract

An integrated approach has been developed for targeted retrieval of microsatellite markers from selected regions of the field bean (Vicia faba L.) genome. The procedure is based on a combination of advanced physical and genetic mapping techniques and includes the following steps: 1) flow-sorting of metaphase chromosomes, 2) construction of microsatellite-enriched chromosome-specific DNA libraries, 3) isolation of polymorphic microsatellite sequences from the libraries, 4) testing chromosome specificity of the microsatellites using polymerase chain reaction with purified fractions of individual chromosome types, and 5) integration of chromosome-specific markers into a genetic map. Several strategies for isolation of microsatellite clones were tested, including direct screening of non-enriched libraries with single or mixed probes and screening of the libraries after one or two rounds of enrichment. Finally, the usefulness of this approach was demonstrated by the retrieval of novel markers from a selected portion of the largest field been chromosome (No. 1).
Features
This publication contains information about 28 features:
Feature NameUniquenameType
GA3GA3genetic_marker
GA4GA4genetic_marker
GA6GA6genetic_marker
GAI1GAI1genetic_marker
GAII12GAII12genetic_marker
GAII3GAII3genetic_marker
GAII30GAII30genetic_marker
GAII5GAII5genetic_marker
GAII54GAII54genetic_marker
GAII59GAII59genetic_marker
GAII67GAII67genetic_marker
GAII68GAII68genetic_marker
GAII8GAII8genetic_marker
GATA11GATA11genetic_marker
GATA2GATA2genetic_marker
GATA3GATA3genetic_marker
GATA5GATA5genetic_marker
JF1-AAC2JF1-AAC2genetic_marker
JF1-AAC3JF1-AAC3genetic_marker
JF1-AG2JF1-AG2genetic_marker
JF1-AG3JF1-AG3genetic_marker
OPA10OPA10genetic_marker
OPN01OPN01genetic_marker
PRX-2PRX-2genetic_marker
SM11SM11genetic_marker

Pages

Featuremaps
This publication contains information about 1 maps:
Map Name
faba-Vf6xVf27-F2
Properties
Additional details for this publication include:
Property NameValue
URLhttp://link.springer.com/article/10.1023/A:1016253214182